Application of red rice seed coat active extract in the preparation of drugs for the prevention and treatment of acute alcoholic liver disease

Abstract

The present invention discloses the use of an active extract of red rice seed coat in the preparation of a drug for preventing and treating acute alcoholic liver disease, wherein the drug is a drug for improving the antioxidant capacity of liver tissue and reducing the generation of free radicals; relieving the pathological damage caused by the infiltration and exudation of inflammatory cells in liver tissue, and improving the liver index and liver function. The active extract of red rice seed coat of the present invention can restore the weight of mice and reduce drunken behavior at a lower dose; improve the antioxidant capacity of liver tissue and reduce the generation of free radicals; relieve the pathological damage caused by the infiltration and exudation of inflammatory cells in liver tissue, and improve the liver index and liver function; and the raw material of the present invention comes from the natural product red rice seed coat, which is not only convenient to collect, but also highly safe.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, and in particular to application of an active extract of red rice seed coat in preparing a medicine for preventing and treating acute alcoholic liver disease. Background Art

[0002] Alcoholic liver disease (ALD) is a disease caused by long-term heavy drinking. It can progress to alcoholic hepatitis, alcoholic liver fibrosis and cirrhosis. Severe alcoholism can induce extensive liver cell necrosis, and finally even alcoholic cirrhosis and liver cancer. The pathogenesis of alcoholic liver disease is not fully understood. It is currently believed that its pathogenesis is mainly related to oxidative stress and lipid peroxidation, cytokine and mitochondrial dysfunction, among which oxidative stress plays a key role in the occurrence and development of acute alcoholic liver injury. The ideal model for studying acute alcoholic liver injury is the animal acute liver injury model. At present, the treatment of alcoholic liver injury is still unsatisfactory, and there is no effective therapeutic drug in clinical practice. In order to prevent or treat the process of acute alcoholic liver injury, it is urgent to find a safe and effective drug for preventing or treating acute alcoholic liver injury from natural products.

[0003] Rice is one of the main food sources for humans. In addition to starch, rice also contains a variety of nutrients, including protein, vitamins, dietary fiber, secondary metabolites such as flavonoids, unsaturated fatty acids, minerals, etc. The active ingredients of red rice seed coat are "natural, nutritious, and multifunctional", and they also have pharmacological effects and health functions such as treatment and prevention for a variety of human diseases. It is reported that red rice seed coat is rich in pigments such as anthocyanins, triterpenes, aromatic oils, flavonoids and other ingredients, and its extracts have antioxidant, anti-fatigue and antiviral activities, and the active extracts of red rice seed coat are safe and have no mutagenic effects. However, there are no patent literature reports on the research on the preventive effect of active extracts of red rice seed coat on acute alcoholic liver injury at home and abroad. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a new use of the active extract of red rice seed coat in medicine, and the active extract of red rice seed coat is used in the preparation of a new drug for preventing and / or treating acute alcoholic liver injury.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] The first object of the present invention is to provide the use of an active extract of red rice seed coat in the preparation of a drug for preventing and treating acute alcoholic liver injury, wherein the drug is a drug for restoring the weight of mice, alleviating drunken behavior; improving the antioxidant capacity of liver tissue and reducing the generation of free radicals; alleviating pathological damage caused by inflammatory cell infiltration and exudation of liver tissue, and improving liver index and liver function.

[0007] Furthermore, the active extracts of red rice seed coat are amino acids and their derivatives, terpenes, lignans and coumarins, organic acids, quinones, benzene and its derivatives, nucleotides and their derivatives, tannins, alkaloids, lipids, phenolic acids and flavonoids; the amino acids and their derivatives are 209, the alkaloids are 173, the lipids are 198, the phenolic acids are 271, the flavonoids are 323, the terpenes are 92, the lignans and coumarins are 63, the organic acids are 121, the quinones are 16, the benzene and its derivatives are 8, the nucleotides and their derivatives are 66, and the tannins are 5.

[0008] In the present invention, the concentration of the active extract of red rice seed coat in the drug for preventing and treating acute alcoholic liver injury is preferably 200 mg / kg to 600 mg / kg per day per animal. The present invention has no special requirements for the dosage form of the drug, the excipients of the drug and the preparation method of the drug, and those familiar to those skilled in the art can be used. The new drug for preventing and / or treating acute alcoholic liver injury of the present invention uses red rice seed coat extract as an active ingredient, and is used to prepare a drug for preventing and treating acute alcoholic liver injury. One or more excipients acceptable to the pharmaceutical preparation may also be added, or it may be compounded with other active ingredients to exert a synergistic effect against acute alcoholic liver injury.

[0009] The present invention has conducted a series of studies on the prevention and treatment of acute alcoholic liver injury with the active extract of red rice seed coat. It has been confirmed that the active extract of red rice seed coat can significantly restore the weight of mice and reduce drunken behavior; it can significantly improve the antioxidant capacity of liver tissue and reduce the generation of free radicals; it can effectively alleviate the pathological damage caused by inflammatory cell infiltration and exudation of liver tissue, and improve liver index and liver function; in general, it effectively improves the liver damage and liver function of mice with ethanol-induced acute alcoholic liver injury model, and the effect on the prevention or treatment of acute alcoholic liver injury is almost equivalent to that of the blank control group. The active extract of red rice seed coat provided by the present invention has a significant effect on the prevention and treatment of acute alcoholic liver injury, and red rice crops are widely distributed, highly safe, low-priced, and the raw materials are easily available; the active extract of red rice seed coat has a good development and application prospect in the preparation of drugs for the prevention and treatment of acute alcoholic liver injury.

[0010] In summary, compared with the prior art, the advantages of the present invention are:

[0011] 1. The present invention has discovered new medical uses for the active extract of red rice seed coat.

[0012] 2. The present invention has found through experiments that the active extract of red rice seed coat can effectively restore the weight of mice at a lower dose, reduce drunken behavior; improve the antioxidant capacity of liver tissue and reduce the generation of free radicals; alleviate the pathological damage caused by inflammatory cell infiltration and exudation of liver tissue, and improve liver index and liver function; the raw materials of the present invention come from natural products, which are easy to collect and have high safety.

[0013] 3. The red rice seed coat active extract of the present invention has a simple extraction process and low cost, and is suitable for industrial production and market promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a liquid quality test result diagram of the red rice seed coat active extract prepared in Example 1 of the present invention;

[0015] Figure 2 This is a curve diagram showing the effect of the active extract of red rice seed coat on the body weight of model mice;

[0016] Figure 3 A bar graph showing the effect of the active extract of red rice seed coat on the postural reflex of model mice;

[0017] Figure 4 The bar graph is a graph showing the effect of the active extract of red rice seed coat on the liver index of model mice;

[0018] Figure 5 The bar graph is a graph showing the effect of the active extract of red rice seed coat on the liver function of model mice;

[0019] Figure 6 A bar graph showing the effect of the active extract of red rice seed coat on oxidative stress-related indicators in liver tissue of model mice;

[0020] Figure 7 This is a HE staining image of the mouse liver tissue pathological section of the present invention. DETAILED DESCRIPTION

[0021] The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without carrying out creative work are within the scope of protection of the present invention. The techniques, methods and equipment known to ordinary technicians in the relevant fields may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be regarded as part of the authorization specification. In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0022] Example 1

[0023] Preparation of the active ingredient extract of red rice seed coat in this example:

[0024] Red rice was purchased from Pu'er, Yunnan. The red rice was crushed and passed through an 80-mesh sieve to obtain red rice powder. 75g of red rice powder was weighed, 1200mL of extractant (the volume ratio of a mixed solution of 0.5% citric acid and 70% ethanol was 3:17) was added, and the mass volume ratio of red rice powder to extractant was 1g:16mL. After fully mixing, ultrasonic was performed at 53°C and 135W power for 30-35min, and extracted 2-3 times. The extracts were combined and centrifuged at a speed of 4500r / min for 10min, and the supernatant was collected, then filtered, and then concentrated by rotary evaporation. The extract was rotary evaporated and the extract was recovered until an aqueous phase was obtained; the aqueous phase was further rotary evaporated to obtain a concentrated solution; the concentrated solution was pre-frozen in a -20°C refrigerator, and then freeze-dried with a freeze dryer to obtain the active ingredient of red rice seed coat (freeze-dried powder).

[0025] The main chemical components of the active ingredient extract of red rice seed coat were qualitatively analyzed by UPLC-MS / MS technology. The analysis results are shown in Figure 1 shown.

[0026] Depend on Figure 1 It can be seen that the active ingredients of red rice seed coat are rich in amino acids and their derivatives, terpenes, lignans and coumarins, alkaloids, lipids, phenolic acids and flavonoids, etc. There are 209 amino acids and their derivatives, 173 alkaloids, 198 lipids, 271 phenolic acids, 323 flavonoids, 92 terpenes, 63 lignans and coumarins, 121 organic acids, 16 quinones, 8 benzene and its derivatives, 66 nucleotides and their derivatives, and 5 tannins.

[0027] The invention provides application of red rice seed coat active extract in preparing a medicine for acute alcoholic liver injury.

[0028] The present invention also provides the use of the active extract of red rice seed coat in preparing medicine for restoring body weight and alleviating drunken behavior.

[0029] The present invention also provides the use of the active extract of red rice seed coat in the preparation of a medicine for improving the antioxidant capacity of liver tissue and reducing the generation of free radicals.

[0030] The present invention also provides the use of the active extract of red rice seed coat in preparing a medicine for alleviating the pathological damage caused by the infiltration and exudation of inflammatory cells in liver tissue and improving liver index and liver function.

[0031] Example 2

[0032] This example is an evaluation of the activity of red rice seed coat active extract in preventing and treating acute alcoholic liver injury:

[0033] 2.1 Establishment of mouse model of acute alcoholic liver injury and experimental process

[0034] C57BL / 6J male mice (8 weeks old, weighing about 20g) were used as the research subjects, with 10 mice in each group. The mice were orally given 45% (v / v) ethanol once by gavage, with a gavage volume of 10mL / kg·BW (equivalent to a dose of 4.48g / kg·d of ethanol) for 14 consecutive days to construct a mouse acute liver injury model. Four hours after the final administration, all mice received 60% ethanol by gavage, except for the normal group and the negative control group, which were given an equal amount of distilled water. The loss and recovery of the righting reflex time caused by ethanol were recorded. The red rice seed coat active ingredient (lyophilized powder) obtained by gavage in Example 1 was used to evaluate its preventive and therapeutic effects on mice with ethanol-induced acute alcoholic liver injury model; the mice were adapted to a standard environment for 7 days after purchase, and the mice were fed with standard feed. The specific experimental process and grouping are as follows:

[0035] Blank control group: The mice were given an equal volume of distilled water by gavage for 14 consecutive days and were free to drink distilled water;

[0036] Negative control group: The mice were intragastrically administered with an equal volume of solvent (10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Sal ine) containing the active ingredients of red rice seed coat for 14 consecutive days, and were allowed to drink distilled water freely.

[0037] Model group: an equal volume of 45% (v / v) ethanol was administered by gavage for 14 consecutive days, and 4 hours after the final administration, all mice received an equal volume of 60% ethanol by gavage;

[0038] Low-dose group of red rice seed coat active ingredient: each mouse was given 200 mg / kg of red rice seed coat active ingredient per day by gavage for 21 consecutive days, and an equal volume of 45% (v / v) ethanol was given by gavage for 14 consecutive days starting from the 8th day. Four hours after the final administration, all mice received an equal volume of 60% ethanol by gavage;

[0039] High-dose group of red rice seed coat active ingredient: each mouse was given 600 mg / kg of red rice seed coat active ingredient per day by gavage for 21 consecutive days, and an equal volume of 45% (v / v) ethanol was given by gavage for 14 consecutive days starting from the 8th day. Four hours after the final administration, all mice received an equal volume of 60% ethanol by gavage;

[0040] Positive control group: Each mouse was given 50 mg / kg of red rice seed coat active ingredient by gavage for 21 consecutive days, and an equal volume of 45% (v / v) ethanol was given by gavage for 14 consecutive days starting from the 8th day. 4 hours after the final administration, all mice received an equal volume of 60% ethanol by gavage.

[0041] 2.2 Detection of the effects of red rice seed coat active extract on body weight, postural reflexes and liver index in mice with acute alcoholic liver injury model:

[0042] 2.2.1 The weight of each group of mice was recorded every day during the experiment. After the modeling was completed, the mice in each group were taken out and laid flat on a flat surface, and they were given time to correct themselves (up to 30 seconds). Criteria for judging the drunken state: After drinking, the mice were placed with their backs facing down and their limbs facing up to observe whether the righting reflex disappeared. If it disappeared and lasted for 30 seconds, it was judged as a drunken state. Determination of sober state: After the mice entered the drunken state, the righting reflex was observed to recover during the observation period. If the turning over time was shorter than 30 seconds, it was judged as a sober state.

[0043] The mice were dissected and the liver tissues were taken out and weighed to obtain the liver index.

[0044] 2.2.2 Experimental results:

[0045] The weight changes of mice can reflect the growth and development of mice. By analyzing the weight changes of mice, the effect of red rice seed coat extract on the growth and development of mice with acute alcoholic liver injury can be evaluated. The duration of the righting reflex can reflect the drunken state of mice. The later the righting reflex is lost, the better the liver function, and the earlier the righting reflex is restored, the better the liver function. The liver index also reflects the damage of the liver. The larger the liver index, the more serious the enlargement of the liver.

[0046] The present invention evaluates the effect of red rice seed coat active extract on acute alcoholic liver injury model mice by measuring the weight change, righting reflex time and liver index of each treated mouse. Figure 2 , Figure 3 , Figure 4 ,in Figure 2 represents the changes in mouse body weight; Figure 3 Indicates the righting reflex of mice; Figure 4 Indicates the liver index of mice. Control is the blank control group; NC is the negative control group; Model is the ethanol-induced model group; RRAL is the low-dose gavage group (200 mg / kg); RRAH is the high-dose gavage group (600 mg / kg); SILY is the positive control group (silymarin 50 mg / kg).

[0047] Depend on Figure 2 It can be seen that the mice given the active extract of red rice seed coat recovered significantly more body weight than the model mice and the effect was similar to that of the positive drug group.

[0048] Depend on Figure 3 It can be seen that the mice given the active extract of red rice seed coat lost their righting reflex later and recovered earlier than those in the model group, which indicates that the drunkenness behavior of mice given the active extract of red rice seed coat was significantly reduced compared with the model group.

[0049] Depend on Figure 4 It can be seen that the liver index of mice given the active extract of red rice seed coat was significantly improved compared with the model group.

[0050] 2.3 Detection of the effects of active ingredients on plasma liver function indicators in model mice:

[0051] 2.3.1 Take blood from the eyeballs of mice in each group using anticoagulant tubes, centrifuge at 3000 rpm for 15 min at 4°C, take the supernatant, and use the corresponding kit to detect the expression of AST and ALT in the plasma of mice in each group.

[0052] 2.3.2 Experimental Results

[0053] AST (aspartate aminotransferase) and ALT (alanine aminotransferase) are sensitive indicators of liver damage. In most cases, the degree of increase in ALT and AST is consistent with the degree of liver cell damage. They are currently the most commonly used indicators for liver function tests. Acute or chronic alcohol administration can cause severe liver oxidative stress, which will lead to cell apoptosis through a variety of mechanisms, including excessive free radicals.

[0054] The present invention uses a corresponding kit to detect the expression of ALT and AST in the plasma of each group of mice to detect the effect of the active extract of red rice seed coat on the liver function of mice with acute alcoholic liver injury. Figure 5 .in Figure 5 The changes in liver function of mice of the present invention are shown in FIG. Figure 5 A represents the ALT content in mouse plasma; Figure 5 B represents the content of AST in mouse plasma; Control is the blank control group; NC is the negative control group; Model is the ethanol-induced model group; RRAL is the low-dose gavage group (200 mg / kg); RRAH is the high-dose gavage group (600 mg / kg); SILY is the positive control group (silymarin 50 mg / kg).

[0055] The experimental results are as follows Figure 3 As shown. Figure 3 It can be seen that the active extract of red rice seed coat significantly inhibited the levels of ALT and AST in the plasma of model mice, indicating that the active extract of red rice seed coat can significantly improve the liver function of mice with acute alcoholic liver injury model.

[0056] 2.4 Detection of the expression of active ingredients on oxidative stress-related indicators in the liver tissue of model mice:

[0057] 2.4.1 Take the liver tissue of each group of mice by aseptic technique, rinse with sterile pre-cooled PBS, take 100 mg of liver tissue and add 900 μl of sterile pre-cooled PBS to homogenize at 4°C, centrifuge to obtain the supernatant, and use the corresponding kit to detect the expression of SOD and ROS in the liver tissue of each group of mice.

[0058] 2.4.2 Experimental Results

[0059] SOD is superoxide dismutase, a metalloproteinase, which can specifically remove harmful free radicals in the body to eliminate the damage caused by free radicals oxidizing certain components in the body. Diseases such as oxygen poisoning, acute inflammation, edema, autoimmune diseases, radiation sickness, etc. are all related to the toxicity of active oxygen.

[0060] Reactive oxygen species (ROS) are products of normal aerobic metabolism in the body. They are a general term for a class of substances that are composed of oxygen, contain oxygen and are active in nature. As a natural byproduct of oxygen metabolism, ROS is at a low level in the body. As a "redox messenger", it participates in intracellular signal transmission and regulation, and plays an important role in the cell cycle, gene expression and maintenance of the body's internal environment homeostasis. However, when the body is stimulated, such as ultraviolet rays, radiation, hypoxia, heat exposure, etc., the level of ROS will increase sharply, exceeding the body's own clearance and processing capacity, the body's oxidation-antioxidation effect will be unbalanced, and oxidative stress will occur, leading to DNA damage, lipid peroxidation, changes in protein structure and function, cell membrane damage, and ultimately causing cell death in the body. In addition, damage to these macromolecules is also related to the pathogenesis of cancer, aging, inflammation and a variety of human diseases (neurodegenerative diseases, cardiovascular diseases and diabetes). Therefore, the level of ROS is an important marker of cellular oxidative damage caused by normal physiological functions of cells and environmental factors.

[0061] The present invention evaluates the effect of red rice seed coat active extract on mice with acute alcoholic liver injury by detecting the expression of SOD and ROS in the liver of each group of mice using the corresponding kit. Figure 6 .in Figure 6 A represents the expression level of SOD in mouse liver; Figure 6 B represents the ROS expression level in mouse liver tissue; Control is the blank control group; NC is the negative control group; Model is the ethanol-induced model group; RRAL is the low-dose gavage group (200 mg / kg); RRAH is the high-dose gavage group (600 mg / kg); SILY is the positive control group (silymarin 50 mg / kg).

[0062] Depend on Figure 6 The results showed that the active extract of red rice seed coat significantly increased the expression of SOD and significantly reduced the level of ROS in the liver tissue of model mice, indicating that the active extract of red rice seed coat can significantly improve the antioxidant capacity of liver tissue and reduce the generation of free radicals.

[0063] 2.5 Detection of the effect of active ingredients on pathological damage of liver tissue in model mice:

[0064] 2.5.1 Take the liver tissues of mice in each group aseptically, rinse them with sterile pre-cooled PBS, fix them and make paraffin sections. After HE staining, observe the changes of tissue structure under a microscope.

[0065] Preparation of paraffin sections:

[0066] (1) Sampling: Fresh liver tissue was fixed with 10% formalin solution for more than 24 hours. The liver tissue was taken out of the fixative and the target area was trimmed with a scalpel in a fume hood. The trimmed tissue and the corresponding label were placed in a dehydration box;

[0067] (2) Dehydration and wax dipping: Place the dehydration box in a dehydrator and dehydrate with graded alcohols in sequence. 75% alcohol for 4 hours, 85% alcohol for 2 hours, 90% alcohol for 2 hours, 95% alcohol for 1 hour, anhydrous ethanol I for 30 minutes, anhydrous ethanol II for 30 minutes, alcohol benzene for 5-10 minutes, xylene I for 5-10 minutes, xylene II for 5-10 minutes, paraffin I melted at 65°C for 1 hour, paraffin II melted at 65°C for 1 hour, paraffin III melted at 65°C for 1 hour;

[0068] (3) Embedding: Embed the wax-soaked tissue in an embedding machine. First, place the melted wax into the embedding frame. Before the wax solidifies, take the tissue out of the dehydration box and place it in the embedding frame according to the requirements of the embedding surface and attach the corresponding label. Cool in a -20℃ freezer. After the wax solidifies, take the wax block out of the embedding frame and trim the wax block;

[0069] (4) Sectioning: Place the trimmed wax block on a paraffin slicer and slice it to a thickness of 4 μm. Float the slices on 40°C warm water on the slide spreader to flatten the tissue. Pick up the tissue with a glass slide and bake the slices in a 60°C oven. After the wax is dried and melted, take them out and store them at room temperature for later use.

[0070] HE staining method is as follows:

[0071] (1) Dewaxing of paraffin sections: Place the sections in xylene I for 20 min, xylene II for 20 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, and 75% alcohol for 5 min, and then wash with tap water;

[0072] (2) Hematoxylin staining: Stain the sections with hematoxylin solution for 3-5 min, wash with tap water, differentiate with differentiation solution, wash with tap water, blue with bluing solution, and rinse with running water;

[0073] (3) Eosin staining: The sections were dehydrated in 85% and 95% graded alcohol for 5 min each, and then stained in eosin solution for 5 min.

[0074] (4) Dehydration and sealing: The sections were placed in anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, anhydrous ethanol III for 5 min, xylene I for 5 min, and xylene II for 5 min to make them transparent, and then sealed with neutral gum;

[0075] (5) Microscope examination, image acquisition and analysis.

[0076] 2.5.2 Experimental Results

[0077] In this experiment, paraffin sections were stained with HE to observe the liver tissue structure of mice. NC was the negative control group; Model was the ethanol-induced model group; RRAL was the low-dose oral gavage group (200 mg / kg); RRAH was the high-dose oral gavage group (600 mg / kg); SILY was the positive control group (silymarin 50 mg / kg). The results are shown in Figure 7 .

[0078] Depend on Figure 7 It can be seen that the active extract of red rice seed coat can significantly reduce the infiltration of inflammatory cells and exudation in the liver of model mice, and maintain the integrity of the liver lobules. This shows that the active extract of red rice seed coat can significantly improve the pathological damage of the liver in model mice.

[0079] In summary, the active extract of red rice seed coat can significantly restore the body weight of model mice and reduce drunken behavior; significantly improve the antioxidant capacity of liver tissue and reduce free radical generation; significantly alleviate the pathological damage caused by inflammatory cell infiltration and exudation of liver tissue, and significantly improve liver index and liver function. The active extract of red rice seed coat provided by the present invention has a significant effect on the prevention and treatment of acute alcoholic liver injury, and the raw materials come from natural products, are highly safe, low cost, and are suitable for industrial production and market promotion applications.

[0080] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0081] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and intent of the present invention.

Claims

1. The use of red rice seed coat active extract in the preparation of a drug for preventing and treating acute alcoholic liver disease, characterized in that: The drug can improve the antioxidant capacity of liver tissue and reduce the generation of free radicals; alleviate the pathological damage caused by inflammatory cell infiltration and exudation of liver tissue, and improve liver index and liver function; The preparation method of the red rice seed coat active extract is as follows: crushing red rice and passing it through an 80-mesh sieve to obtain a powder containing red rice seed coat; weighing 75g of the powder containing red rice seed coat, adding 1200mL of an extractant, wherein the mass volume ratio of the powder containing red rice seed coat to the extractant is 1g:16mL, fully mixing, ultrasonicating for 30-35min at 53°C and 135W power, extracting 2-3 times, combining the extracts and centrifuging at a speed of 4500r / min for 10min, collecting the supernatant, and then filtering; concentrating the supernatant by rotary evaporation, rotary evaporating the extract, and recovering the extractant until an aqueous phase is obtained; continuing to rotary evaporate and concentrate the aqueous phase to obtain a concentrated solution; pre-freezing the concentrated solution in a -20°C refrigerator, and then freeze-drying it with a freeze dryer to obtain the red rice seed coat active component extract; The extractant is a mixed solution of 0.5% citric acid and 70% ethanol in a volume ratio of 3:17.

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