A temporary storage device for bovine reovirus RT-PCR test

Abstract

The present invention belongs to the field of virus storage technology, and in particular, a temporary storage device for bovine reovirus RT-PCR testing, comprising a storage tank temporarily used to store bovine reovirus samples and a reagent tube containing the bovine reovirus samples, wherein the interior of the storage tank is provided with a driving cavity and an action cavity from bottom to top, respectively, the inner bottom wall of the action cavity is in sliding contact with a support base plate, a support column is fixedly sleeved on the surface at the center of the support base plate, a rotating mechanism is provided on the outer surface of the support column, the rotating mechanism is connected to a shaking mechanism, and a height adjustment mechanism is provided inside the driving cavity. The temporary storage device for bovine reovirus RT-PCR testing drives the driving bevel gear to rotate around the center of the support column when the driving ring rotates, and because the driving bevel gear is meshed with the outer surface of the linkage bevel gear, the driving bevel gear can drive the linkage shaft to rotate when the driving bevel gear rotates, thereby driving the cam rod to rotate and realizing the up and down movement of the traction rod.

Description

Technical Field

[0001] The present invention relates to the technical field of virus storage, in particular to a temporary storage device for bovine reovirus RT-PCR test. Background Art

[0002] RT-PCR is a technology that combines reverse transcription (RT) of RNA and polymerase chain amplification (PCR) of cDNA. It is a commonly used experimental technique for detecting bovine reovirus. For temporary storage devices for bovine reovirus RT-PCR tests, a common option is to use cold chain transportation and storage equipment to ensure the stability and safety of samples.

[0003] When conducting RT-PCR tests on bovine reovirus, the virus cannot be used up all at once. Therefore, unused viruses need to be temporarily stored. The commonly used storage method is to stack the reagent tubes containing the virus in a storage cabinet, and the storage cabinet is maintained at a specified temperature by an air conditioning device. This storage method is a static storage of the vaccine. After a certain period of storage, the virus sample in the reagent tube will precipitate, which will cause experimental errors when the experiment is taken out. At the same time, the reagent tube is temporarily stored in a low-temperature environment. However, if the low-temperature environment is maintained in the storage tank for a long time and the virus sample is then temporarily stored therein, it will cause a waste of resources. Therefore, the present invention solves the shortcomings of the above-mentioned technical problems. Summary of the Invention

[0004] Based on the above-mentioned existing technical problems, the present invention proposes a temporary storage device for bovine reovirus RT-PCR test.

[0005] The present invention proposes a temporary storage device for bovine reovirus RT-PCR test, comprising a storage tank temporarily used to store bovine reovirus samples and a reagent tube containing the bovine reovirus samples, a sealing cover being provided on top of the storage tank, a driving cavity and an action cavity being respectively provided inside the storage tank from bottom to top, the inner bottom wall of the action cavity being in sliding contact with a supporting base plate, a support column being fixedly sleeved on the surface at the center of the circle of the support base plate, a rotating mechanism being provided on the outer surface of the support column, the rotating mechanism being connected to a shaking mechanism, and a height adjustment mechanism being provided inside the driving cavity.

[0006] The rotating mechanism rotates 360° inside the action cavity, so that the reagent tube stored in the action cavity maintains a circumferential rotation around the center of the support column.

[0007] The shaking mechanism drives the reagent tube to rotate circumferentially, so that the reagent tube shakes up and down while rotating.

[0008] The height adjustment device adjusts the height of the supporting base plate, thereby lifting the reagent tube out of the action cavity and taking the reagent tube.

[0009] Preferably, the rotating mechanism includes a rotating bearing fixedly connected to the upper surface of the support base plate, the upper surface of the inner ring of the rotating bearing is fixedly connected to a ring-shaped connecting base plate, and the outer surface of the upper end of the support column is rotatably sleeved with a rotating ring through a limiting ring.

[0010] Through the above technical solution, the reagent tubes are distributed in a circular array inside the action cavity. In order to make them rotate circumferentially and avoid the precipitation of the bovine reovirus samples in the reagent tubes, they are made to rotate circumferentially at a uniform speed in the action cavity. The centrifugal force can prevent the virus samples in the reagent tubes from static precipitation. Therefore, the connecting base plate and the rotating ring rotate synchronously, so that the reagent tubes between the two are linked. The limit ring can limit the position of the rotating ring. At the same time, the rotating bearing can keep the connecting base plate rotating on the surface of the non-rotating support base plate.

[0011] Preferably, the rotation mechanism also includes connecting plates distributed in a circular array and fixedly connected to the outer surface of the rotating ring, the opposite side surfaces of adjacent connecting plates are fixedly connected with mounting plates, the side surface of the connecting plate away from the support column is fixedly connected with a connecting ring, the outer surfaces of the connecting ring and the support base plate are both rotatably sleeved with circular rings, the outer surfaces of the two circular rings are both distributed in a circular array and fixedly connected with guide blocks, and the inner wall of the action cavity is provided with a guide groove that is slidably engaged with the outer surface of the guide block.

[0012] Through the above technical solution, in order to install the shaking mechanism, the shaking mechanism is installed on the mounting plate connected to the connecting plate, so that when the mounting plate rotates, the shaking mechanism installed on the upper surface of the mounting plate controls the shaking of the reagent tube, and in order to guide the reagent tube when adjusting the height, and at the same time enable the rotating mechanism and the shaking mechanism to move stably, when the connecting ring rotates with the rotating ring, the connecting ring rotates on the inner surface of the circular ring, and when the support base and the rotating ring connected by the support column are adjusted in height, the guide block and the guide groove can cooperate to realize the position limitation of the circular ring.

[0013] Preferably, the rotation mechanism also includes a ring-shaped drive ring fixedly connected to the mounting plate and the lower surface of the connecting plate, the inner surface of the drive ring is fixedly connected to an inner rack, the upper surface of the support base plate close to the support column is fixedly connected to a reduction motor, the outer surface of the output shaft of the reduction motor is fixedly connected to a rotating rod through a coupling, the upper surface of the rotating rod is fixedly connected to a driving gear, the outer surface of the driving gear is meshed with the surface of the inner rack, the side surface of the support column close to the rotating rod is fixedly connected to a T-shaped tube, and the inner surface of the T-shaped tube is movably connected to the outer surface of the rotating rod.

[0014] Through the above technical solution, in order to drive the rotating ring to rotate on the outer surface of the support column and realize the rotation adjustment of the reagent tube, the reduction motor installed on the support base plate drives the rotating rod to rotate, so that the driving gear on its outer surface can drive the driving ring with the inner rack to rotate, so that the driving ring drives the rotating ring to realize circumferential rotation through the connecting plate and the mounting plate, thereby driving the reagent tubes distributed in the circular array to rotate around the center of the circle. In order to realize force support for the rotating rod extending to the bottom of the connecting ring, the T-shaped tube is movably connected to the middle outer surface of the rotating rod to ensure the stability and normal operation of the rotating mechanism.

[0015] Preferably, the rocking mechanism includes a supporting side plate fixedly mounted on the upper surface of the corresponding mounting plate, the middle surface of the supporting side plate is rotatably connected to a linkage shaft, and the outer surface of the linkage shaft at one end away from the support column is fixedly connected to a cam rod.

[0016] Through the above technical solution, in order to shake the rotating reagent tube up and down to avoid the precipitation of the virus sample stored therein, the linkage shaft installed on the supporting side plate is rotated 360°, which can drive the cam rod to rotate synchronously, so that the unconnected end of the cam rod can pull the hinged reagent tube to achieve up and down shaking.

[0017] Preferably, a driving bevel gear is fixedly sleeved on the outer surface of one end of the linkage shaft close to the support column, a linkage bevel gear is fixedly sleeved on the outer surface of the upper end of the support column, the outer surfaces of multiple driving bevel gears are meshed with the outer surface of the linkage bevel gear, and the side surface of the unconnected end of the cam rod is rotatably connected to a T-shaped traction rod.

[0018] Through the above technical solution, in order to make multiple cam rods rotate synchronously, the driving ring drives the driving bevel gear to rotate around the center of the support column when it rotates, and because the driving bevel gear is engaged with the outer surface of the linkage bevel gear, the linkage shaft can be driven to rotate when the driving bevel gear rotates, thereby driving the cam rod to rotate and realizing the up and down movement of the traction rod.

[0019] Preferably, the upper surface of the connecting base plate is fixedly connected with telescopic rods distributed in a circular array, the outer surface of the telescopic rods is fixedly sleeved with a buffer spring, and the opposite side surfaces of the telescopic rods and the traction rod are fixedly connected with a clamping cylinder.

[0020] Through the above technical solution, in order to support and buffer the up and down movement of the traction rod, a telescopic rod is set on the lower surface of the clamping tube at the lower end, so that it can be extended and retracted as the traction rod moves up and down, and the telescopic buffering of the telescopic rod is achieved through the buffer spring. In order to temporarily place and store the reagent tube, two clamping tubes are set to clamp and fix the upper and lower end outer surfaces of the reagent tube.

[0021] Preferably, the shaking mechanism further comprises vacuum suction cups distributed in a circular array and fixedly connected to the inner wall of the clamping cylinder, an air pipe is installed on the outer surface of the clamping cylinder, and a miniature ultrasonic generator is fixedly installed on the outer surface of the clamping cylinder.

[0022] Through the above technical solution, in order to quickly clamp the placed reagent tube, the vacuum suction cup generates negative pressure through the air pipe, and then the reagent tube placed in the clamping cylinder can be quickly clamped. In order to improve the mixing of the virus sample in the reagent tube, a miniature ultrasonic generator is provided. The miniature ultrasonic generator is a small-sized, low-power ultrasonic generator that converts electrical energy into mechanical energy to generate high-frequency vibrations and transmits the vibrations to the object being processed through the medium, so that the vibrations can be transmitted to the virus sample in the reagent tube through the vacuum suction cup.

[0023] Preferably, the outer surfaces of the upper and lower clamping cylinders are fixedly connected with spiral-shaped evaporative cooling belts, a battery is installed on the outer surface of one of the clamping cylinders, the power leads of the evaporative cooling belt are connected to the positive and negative poles of the battery, and the outer surface of the storage tank is distributed in a circular array with ventilation holes.

[0024] Through the above technical solution, in order to ensure the activity of the virus sample, the reagent tube needs to be temporarily stored in a low-temperature environment. However, if the low-temperature environment is maintained for a long time in the storage tank and the virus sample is temporarily stored therein, it will cause a waste of resources and generate a lot of heat energy. Therefore, in order to keep the inside of the temporarily stored reagent tube at a low temperature, an evaporative refrigeration belt is provided. The evaporative refrigeration belt is a belt-shaped device with an evaporative refrigeration principle, which is used to achieve a refrigeration effect. It is usually composed of a heat-conducting material and a structure containing a refrigerant inside. The working principle of the evaporative refrigeration belt is to use the heat absorption property of the liquid when it evaporates for cooling. Inside the refrigeration belt, there is a refrigerant, which is usually a volatile liquid such as ammonia or acetone. When the refrigeration belt is exposed to the air, the refrigerant will begin to evaporate and absorb the surrounding heat. This process will cause the surface temperature of the refrigeration belt to drop, thereby achieving the effect of lowering the surrounding temperature. The battery can drive the evaporative refrigeration belt to operate, and the vent can expose the refrigeration belt to the air.

[0025] Preferably, the height adjustment mechanism includes a rotating screw rotatably connected to the inner bottom wall of the driving cavity through a bearing, the outer surface of the rotating screw extends to the outer surface of the action cavity and is sleeved with the internal thread of the support column, the outer surface of the lower end of the rotating screw is fixedly sleeved with a driven gear, the inner bottom wall of the driving cavity is fixedly connected to a rotating motor, the outer surface of the output shaft of the reduction motor is fixedly connected to a driving gear through a coupling, and the outer surface of the driving gear is meshed with the outer surface of the driven gear.

[0026] Through the above technical solution, in order to facilitate the use of reagent tubes at any time, the support column drives the rotating mechanism and the shaking mechanism to rise when taking the reagent tube, so that the driving gear is driven to rotate by the rotating motor, and the driven gear engaged therewith drives the rotating screw to rotate, so that the support column with a threaded sleeve on the outer surface can be raised, and the reagent tube can be extended out of the action cavity, so that it can be conveniently taken.

[0027] The beneficial effects of the present invention are:

[0028] 1. By setting up a rotating mechanism, the reagent tubes containing virus samples temporarily stored can be rotated at a uniform speed. During the adjustment process, the reduction motor drives the rotating rod to rotate, so that the driving gear on its outer surface drives the driving ring with an internal rack to rotate, so that the driving ring drives the rotating ring to achieve circumferential rotation through the connecting plate and the mounting plate. Therefore, the reagent tubes distributed in the ring array can be driven to rotate around the center of the circle, thereby avoiding precipitation of the reagent tubes due to static storage.

[0029] 2. By setting up a shaking mechanism, the rotating reagent tube can be shaken up and down. During the adjustment process, the driving ring drives the driving bevel gear to rotate around the center of the support column. Because the driving bevel gear is engaged with the outer surface of the linkage bevel gear, the driving bevel gear can drive the linkage shaft to rotate when it rotates, thereby driving the cam rod to rotate and realize the up and down movement of the traction rod. The evaporation cooling belt during shaking can achieve the effect of lowering the ambient temperature of the reagent tube, thereby facilitating the stability of virus storage and keeping the virus sample mixed when it is taken out at any time.

[0030] 3. By setting up a height adjustment mechanism, temporarily stored reagent tubes can be easily taken out at any time. During the adjustment process, the driving gear is driven by the rotating motor to rotate, so that the driven gear engaged therewith drives the rotating screw to rotate, thereby causing the support column with a threaded sleeve on the outer surface to rise, so that the reagent tube extends out of the action cavity, making it easy to take it out. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0032] Figure 2 A three-dimensional diagram of the support column structure of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0033] Figure 3 A three-dimensional diagram of a circular structure of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0034] Figure 4 This is a three-dimensional diagram of the connection base structure of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0035] Figure 5 A three-dimensional diagram of the telescopic rod structure of a temporary storage device for bovine reovirus RT-PCR testing proposed by the present invention;

[0036] Figure 6 This is a three-dimensional diagram of the height adjustment mechanism of a temporary storage device for bovine reovirus RT-PCR testing proposed by the present invention;

[0037] Figure 7 A three-dimensional diagram of the evaporative cooling belt structure of a temporary storage device for bovine reovirus RT-PCR testing proposed by the present invention;

[0038] Figure 8 A three-dimensional diagram of the structure of the linked bevel gears of a temporary storage device for bovine reovirus RT-PCR testing proposed by the present invention;

[0039] Figure 9 A three-dimensional diagram of the drive ring structure of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0040] Figure 10 A three-dimensional diagram of the structure of a clamping cylinder of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention;

[0041] Figure 11 This is a three-dimensional diagram of the vacuum suction cup structure of a temporary storage device for bovine reovirus RT-PCR test proposed by the present invention.

[0042] Figure: 1, storage tank; 11, sealing cover; 12, driving chamber; 13, action chamber; 14, supporting base plate; 15, supporting column; 2, reagent tube; 3, rotating mechanism; 31, rotating bearing; 32, connecting base plate; 33, rotating ring; 34, connecting plate; 35, mounting plate; 36, connecting ring; 37, circular ring; 38, guide block; 39, guide groove; 40, driving ring; 41, inner rack; 42, reduction motor; 43, rotating rod; 44, driving gear; 45, T-shaped tube ; 5. Rocking mechanism; 51. Support side plate; 52. Linkage shaft; 53. Cam rod; 54. Driving bevel gear; 55. Linkage bevel gear; 56. Traction rod; 57. Telescopic rod; 58. Buffer spring; 59. Clamping cylinder; 60. Vacuum suction cup; 61. Trachea; 62. Micro ultrasonic generator; 63. Evaporative cooling belt; 64. Battery; 65. Vent; 7. Height adjustment mechanism; 71. Rotating screw; 72. Driven gear; 73. Driving gear; 74. Rotating motor. DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0044] Reference Figures 1-11 A temporary storage device for bovine reovirus RT-PCR test includes a storage tank 1 for temporarily storing bovine reovirus samples and a reagent tube 2 containing the bovine reovirus samples. A sealing cover 11 is provided on the top of the storage tank 1. A driving cavity 12 and an action cavity 13 are respectively provided inside the storage tank 1 from bottom to top. The inner bottom wall of the action cavity 13 is in sliding contact with a supporting base plate 14. A support column 15 is fixedly sleeved on the surface at the center of the support base plate 14. A rotating mechanism 3 is provided on the outer surface of the support column 15. The rotating mechanism 3 is connected to a shaking mechanism 5. A height adjustment mechanism 7 is provided inside the driving cavity 12.

[0045] The rotating mechanism 3 rotates 360° inside the action cavity 13 , so that the reagent tube 2 stored in the action cavity 13 keeps rotating circumferentially around the center of the support column 15 .

[0046] The reagent tubes 2 are distributed in a circular array inside the action cavity 13. In order to make them rotate circumferentially and avoid the precipitation of the bovine reovirus sample in the reagent tube 2, they are made to rotate circumferentially at a uniform speed in the action cavity 13. The centrifugal force can prevent the virus sample in the reagent tube 2 from settling. Therefore, the rotating mechanism 3 includes a rotating bearing 31 fixedly connected to the upper surface of the support base plate 14. The upper surface of the inner ring of the rotating bearing 31 is fixedly connected to a ring-shaped connecting base plate 32. The outer surface of the upper end of the support column 15 is rotatably sleeved with a rotating ring 33 through a limiting ring. The connecting base plate 32 and the rotating ring 33 rotate synchronously, so that the reagent tubes 2 between the two are linked. The limiting ring can limit the position of the rotating ring 33. At the same time, the rotating bearing 31 can keep the connecting base plate 32 rotating on the upper surface of the non-rotating support base plate 14.

[0047] In order to install the shaking mechanism 5, the rotating mechanism 3 also includes connecting plates 34 distributed in an annular array and fixedly connected to the outer surface of the rotating ring 33. The opposite side surfaces of adjacent connecting plates 34 are fixedly connected with mounting plates 35. The side surface of the connecting plate 34 away from the support column 15 is fixedly connected with a connecting ring 36. The outer surfaces of the connecting ring 36 and the supporting base plate 14 are both rotatably sleeved with a circular ring 37. The shaking mechanism 5 is installed on the mounting plate 35 so that when the mounting plate 35 rotates, the shaking mechanism 5 installed on its upper surface controls the shaking of the reagent tube 2, and in order to perform In order to provide guidance when adjusting the height and enable the rotating mechanism 3 and the shaking mechanism 5 to move stably, the outer surfaces of the two rings 37 are distributed in a circular array and are fixedly connected with guide blocks 38. The inner wall of the action cavity 13 is provided with a guide groove 39 that is slidably engaged with the outer surface of the guide block 38. When the connecting ring 36 rotates with the rotating ring 33, the connecting ring 36 rotates on the inner surface of the ring 37. When the support base and the rotating ring 33 connected by the support column 15 are adjusted in height, the guide block 38 and the guide groove 39 can be coordinated to limit the position of the ring 37.

[0048] In order to drive the rotating ring 33 to rotate on the outer surface of the support column 15 to realize the rotation adjustment of the reagent tube 2, the rotating mechanism 3 also includes a ring-shaped driving ring 40 fixedly connected to the lower surface of the mounting plate 35 and the connecting plate 34, the inner surface of the driving ring 40 is fixedly connected to the inner rack 41, the upper surface of the support base 14 close to the support column 15 is fixedly connected to the reduction motor 42, the outer surface of the output shaft of the reduction motor 42 is fixedly connected to the rotating rod 43 through a coupling, the upper surface of the rotating rod 43 is fixedly connected to the driving gear 44, the outer surface of the driving gear 44 is meshed with the surface of the inner rack 41, and the reduction motor 42 installed on the support base 14 drives the rotating rod 43 to rotate. The drive ring 40 with the inner rack 41 can rotate, so that the drive gear 44 on its outer surface can drive the drive ring 40 with the inner rack 41 to rotate, so that the drive ring 40 drives the rotating ring 33 to realize circumferential rotation through the connecting plate 34 and the mounting plate 35, thereby driving the reagent tubes 2 distributed in the annular array to rotate around the center of the circle. In order to realize force support for the rotating rod 43 extending to the bottom of the connecting ring 36, a T-shaped tube 45 is fixedly connected to the side surface of the support column 15 close to the rotating rod 43, and the inner surface of the T-shaped tube 45 is movably connected to the outer surface of the rotating rod 43. The T-shaped tube 45 is movably connected to the middle outer surface of the rotating rod 43 to ensure the stability and normal operation of the rotating mechanism 3.

[0049] By providing a rotating mechanism 3, the reagent tubes 2 containing virus samples temporarily stored can be rotated at a uniform speed. During the adjustment process, the reduction motor 42 drives the rotating rod 43 to rotate, so that the driving gear 44 on its outer surface can drive the driving ring 40 with the inner rack 41 to rotate, so that the driving ring 40 drives the rotating ring 33 to achieve circumferential rotation through the connecting plate 34 and the mounting plate 35, thereby driving the reagent tubes 2 distributed in the annular array to rotate around the center of the circle, thereby avoiding precipitation of the reagent tubes 2 due to static storage.

[0050] The shaking mechanism 5 drives the reagent tube 2 to rotate in the circumferential direction, so that the reagent tube 2 shakes up and down while rotating.

[0051] In order to shake the rotating reagent tube 2 up and down to avoid the precipitation of the virus sample stored therein, the shaking mechanism 5 includes a supporting side plate 51 fixedly installed on the upper surface of the corresponding mounting plate 35. The middle surface of the supporting side plate 51 is rotatably connected to a linkage shaft 52. The outer surface of the end of the linkage shaft 52 away from the support column 15 is fixedly connected to a cam rod 53. The linkage shaft 52 installed on the supporting side plate 51 rotates 360°, which can drive the cam rod 53 to rotate synchronously, so that the unconnected end of the cam rod 53 can pull the hinged reagent tube 2 to achieve up and down shaking.

[0052] In order to make multiple cam rods 53 rotate synchronously, a driving bevel gear 54 is fixedly sleeved on the outer surface of one end of the linkage shaft 52 close to the support column 15, and a linkage bevel gear 55 is fixedly sleeved on the outer surface of the upper end of the support column 15. The outer surfaces of multiple driving bevel gears 54 are meshed with the outer surface of the linkage bevel gear 55. The side surface of the unconnected end of the cam rod 53 is rotatably connected with a T-shaped traction rod 56. When the drive ring 40 rotates, it drives the driving bevel gear 54 to rotate around the center of the support column 15. Because the driving bevel gear 54 is meshed with the outer surface of the linkage bevel gear 55, the driving bevel gear 54 can drive the linkage shaft 52 to rotate when it rotates, thereby driving the cam rod 53 to rotate and realizing the up and down movement of the traction rod 56.

[0053] In order to support and buffer the up and down movement of the traction rod 56, the upper surface of the connecting base plate 32 is fixedly connected with telescopic rods 57 in a circular array, and the outer surface of the telescopic rod 57 is fixedly sleeved with a buffer spring 58. The surfaces on the opposite sides of the telescopic rod 57 and the traction rod 56 are fixedly connected with a clamping tube 59. The telescopic rod 57 is set on the lower surface of the clamping tube 59 at the lower end, so that it can be extended and retracted as the traction rod 56 moves up and down, and the expansion and contraction buffering of the telescopic rod 57 is realized by the buffer spring 58. The two clamping tubes 59 clamp and fix the upper and lower end outer surfaces of the reagent tube 2.

[0054] In order to quickly clamp the placed reagent tube 2, the shaking mechanism 5 also includes a vacuum suction cup 60 distributed in a circular array and fixedly connected to the inner wall of the clamping tube 59. The outer surface of the clamping tube 59 is installed with an air pipe 61, and the vacuum suction cup 60 is used to generate negative pressure through the air pipe 61, and then the reagent tube 2 placed in the clamping tube 59 can be quickly clamped. In order to improve the mixing of the virus sample in the reagent tube 2, a miniature ultrasonic generator 62 is fixedly installed on the outer surface of the clamping tube 59. The miniature ultrasonic generator 62 is a small-sized, low-power ultrasonic generator that converts electrical energy into mechanical energy to generate high-frequency vibrations and transmits the vibrations to the object being processed through the medium, so that the vibrations can be transmitted to the virus sample in the reagent tube 2 through the vacuum suction cup 60.

[0055] In order to ensure the activity of the virus sample, the reagent tube 2 needs to be temporarily stored in a low-temperature environment. However, if the storage tank 1 maintains a low-temperature environment for a long time and then temporarily stores the virus sample, it will cause a waste of resources and generate more heat energy. In order to keep the interior of the temporarily stored reagent tube 2 at a low temperature, the outer surfaces of the upper and lower clamping cylinders 59 are fixedly connected with a spiral evaporative cooling belt 63, and a battery 64 is installed on the outer surface of one of the clamping cylinders 59. The power lead of the evaporative cooling belt 63 is connected to the positive and negative electrodes of the battery 64. The outer surface of the storage tank 1 is distributed in a ring array with vent holes 65, and the evaporative cooling belt 63 is connected to the positive and negative electrodes of the battery 64. Belt 63 is a belt-shaped device with an evaporative refrigeration principle, which is used to achieve a cooling effect. It is usually composed of a heat-conducting material and a structure containing a refrigerant inside. The working principle of the evaporative refrigeration belt 63 is to use the heat absorption property of the liquid when it evaporates to perform cooling. Inside the refrigeration belt, there is a refrigerant, which is usually a volatile liquid such as ammonia or acetone. When the refrigeration belt is exposed to the air, the refrigerant will begin to evaporate and absorb the surrounding heat. This process will cause the surface temperature of the refrigeration belt to drop, thereby achieving the effect of lowering the ambient temperature. The battery 64 can drive the evaporative refrigeration belt 63 to operate, and the vent 65 can expose the refrigeration belt to the air.

[0056] By setting up the shaking mechanism 5, the rotating reagent tube 2 can be shaken up and down. During the adjustment process, the driving ring 40 drives the driving bevel gear 54 to rotate around the center of the support column 15. Because the driving bevel gear 54 is engaged with the outer surface of the linkage bevel gear 55, the driving bevel gear 54 can drive the linkage shaft 52 to rotate when it rotates, thereby driving the cam rod 53 to rotate and realizing the up and down movement of the traction rod 56. When shaking, the evaporation refrigeration belt 63 achieves the effect of lowering the ambient temperature of the reagent tube 2, thereby facilitating the stability of virus storage and keeping the virus sample mixed when it is taken out at any time.

[0057] The height adjustment device adjusts the height of the supporting base plate 14 , thereby lifting the reagent tube 2 out of the action cavity 13 and taking the reagent tube 2 .

[0058] The outer surface of the rotating screw 71 extends to the outer surface of the action cavity 13 and is sleeved with the internal thread of the support column 15. The outer surface of the lower end of the rotating screw 71 is fixedly sleeved with a driven gear 72. The inner bottom wall of the drive cavity 12 is fixedly connected to a rotating motor 74. The outer surface of the output shaft of the reduction motor 42 is fixedly connected to a driving gear 73 through a coupling. The outer surface of the driving gear 73 is meshed with the outer surface of the driven gear 72. The driving gear 73 is driven by the rotating motor to rotate, so that the meshed driven gear 72 drives the rotating screw 71 to rotate, thereby making the support column 15 with the outer surface threaded sleeve rise, so that the reagent tube 2 extends out of the action cavity 13, so that it can be convenient to take it.

[0059] By providing a height adjustment mechanism 7, the temporarily stored reagent tubes 2 can be easily accessed at any time. During the adjustment process, the driving gear 73 is driven to rotate by the rotating motor 74, so that the driven gear 72 engaged therewith drives the rotating screw 71 to rotate, thereby causing the support column 15 with a threaded sleeve on the outer surface to rise, so that the reagent tube 2 extends out of the action cavity 13, making it convenient to take it out.

[0060] Working principle: Figures 1-11 As shown, in a specific embodiment of the present invention, the rotary motor 74 inside the driving cavity 12 is driven to operate, so that its output shaft drives the driving gear 73 to rotate, so that the driven gear 72 engaged therewith drives the rotating screw 71 to rotate, thereby causing the support column 15 with the outer surface threaded sleeve to rise, so that the clamping cylinder 59 is exposed from the operating cavity 13;

[0061] Then, the reagent tube 2 containing the virus sample is sequentially passed through the evaporative cooling belt 63 and inserted into the clamping cylinder 59. After the vacuum suction cup 60 generates negative pressure through the air pipe 61, the reagent tube 2 placed in the clamping cylinder 59 can be quickly clamped. Then, the support column 15 drives the reagent tube 2 to return to its original position, so that the reagent tube 2 is inside the action cavity 13, and the sealing cover 11 is closed to seal the storage tank 1.

[0062] During the temporary storage of the reagent tube 2 containing the virus sample, the reduction motor 42 mounted on the support base 14 drives the rotating rod 43 in the T-shaped tube 45 to rotate, thereby causing the driving gear 44 on its outer surface to drive the driving ring 40 with the inner rack 41 to rotate, so that the driving ring 40 drives the rotating ring 33 to achieve circumferential rotation through the connecting plate 34 and the mounting plate 35. At the same time, the connecting ring 36 rotates on the inner surface of the circular ring 37, so that the reagent tube 2 can be rotated inside the action cavity 13;

[0063] When the drive ring 40 rotates, it drives the driving bevel gear 54 to rotate around the center of the support column 15. Because the driving bevel gear 54 is meshed with the outer surface of the linkage bevel gear 55, the driving bevel gear 54 can drive the linkage shaft 52 to rotate when the driving bevel gear 54 rotates, thereby driving the cam rod 53 to rotate and realize the up and down movement of the traction rod 56. At the same time, the telescopic rod 57 is extended and retracted as the traction rod 56 moves up and down, and the extension and retraction of the telescopic rod 57 is buffered by the buffer spring 58.

[0064] When the reagent tube 2 is shaken up and down, the micro ultrasonic generator 62 converts electrical energy into mechanical energy to generate high-frequency vibration, and the vibration can be transmitted to the virus sample in the reagent tube 2 through the vacuum suction cup 60, thereby preventing the generation of virus precipitation. At the same time, when the reagent tube 2 is shaken, the refrigeration belt 63 evaporates to achieve the effect of lowering the ambient temperature of the reagent tube 2, thereby facilitating the stability of virus storage and keeping the virus sample mixed when it is taken out at any time.

[0065] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A temporary storage device for a bovine reovirus RT-PCR test, comprising a storage tank (1) for temporarily storing a bovine reovirus sample and a reagent tube (2) containing the bovine reovirus sample, characterized in that: A sealing cover (11) is provided above the storage tank (1), and a driving cavity (12) and an action cavity (13) are respectively provided inside the storage tank (1) from bottom to top. The inner bottom wall of the action cavity (13) is in sliding contact with a support base plate (14), and a support column (15) is fixedly sleeved on the surface at the center of the circle of the support base plate (14). The outer surface of the upper end of the support column (15) is rotatably sleeved with a rotating ring (33) through a limiting ring. A rotating mechanism (3) is provided on the outer surface of the support column (15), and the rotating mechanism (3) is connected to a shaking mechanism (5). A height adjustment mechanism (7) is provided inside the driving cavity (12); The rotating mechanism (3) rotates 360° inside the action cavity (13), thereby enabling the reagent tube (2) stored in the action cavity (13) to maintain a circumferential rotation around the center of the support column (15); The rotating mechanism (3) further comprises connecting plates (34) which are fixedly connected to the outer surface of the rotating ring (33) in an annular array, a mounting plate (35) is fixedly connected to the surface of the opposite side of the adjacent connecting plates (34), a connecting ring (36) is fixedly connected to the surface of the connecting plate (34) away from the support column (15), the outer surfaces of the connecting ring (36) and the supporting base plate (14) are both rotatably sleeved with circular rings (37), the outer surfaces of the two circular rings (37) are both fixedly connected to guide blocks (38) in an annular array, and the inner wall of the action cavity (13) is provided with a guide groove (39) which is slidably engaged with the outer surface of the guide block (38); The rotating mechanism (3) further comprises a ring-shaped driving ring (40) fixedly connected to the lower surface of the mounting plate (35) and the connecting plate (34), the inner surface of the driving ring (40) being fixedly connected to an inner rack (41), the upper surface of the support base plate (14) close to the support column (15) being fixedly connected to a reduction motor (42), the outer surface of the output shaft of the reduction motor (42) being fixedly connected to a rotating rod (43) via a coupling, the upper surface of the rotating rod (43) being fixedly connected to a driving gear (44), the outer surface of the driving gear (44) being meshed with the surface of the inner rack (41), the side surface of the support column (15) close to the rotating rod (43) being fixedly connected to a T-shaped tube (45), the inner surface of the T-shaped tube (45) being movably sleeved with the outer surface of the rotating rod (43); The shaking mechanism (5) drives the reagent tube (2) to rotate in the circumferential direction, so that the reagent tube (2) shakes up and down while rotating; The rocking mechanism (5) comprises a supporting side plate (51) fixedly mounted on the upper surface of the corresponding mounting plate (35); a linkage shaft (52) is rotatably connected to the middle surface of the supporting side plate (51); and a cam rod (53) is fixedly connected to the outer surface of one end of the linkage shaft (52) away from the supporting column (15); The height adjustment mechanism (7) adjusts the height of the support base plate (14), thereby lifting the reagent tube (2) out of the action cavity (13) and taking the reagent tube (2).

2. A temporary storage device for bovine reovirus RT-PCR test according to claim 1, characterized in that: The rotating mechanism (3) comprises a rotating bearing (31) fixedly connected to the upper surface of the supporting base plate (14), and a ring-shaped connecting base plate (32) is fixedly connected to the upper surface of the inner ring of the rotating bearing (31).

3. A temporary storage device for bovine reovirus RT-PCR test according to claim 2, characterized in that: A driving bevel gear (54) is fixedly sleeved on the outer surface of one end of the linkage shaft (52) close to the support column (15), and a linkage bevel gear (55) is fixedly sleeved on the outer surface of the upper end of the support column (15). The outer surfaces of the plurality of driving bevel gears (54) are meshed with the outer surfaces of the linkage bevel gears (55), and a T-shaped traction rod (56) is rotatably connected to the surface of one side of the unconnected end of the cam rod (53).

4. A temporary storage device for bovine reovirus RT-PCR test according to claim 3, characterized in that: Telescopic rods (57) are fixedly connected to the upper surface of the connecting base plate (32) in a circular array. Buffer springs (58) are fixedly sleeved on the outer surfaces of the telescopic rods (57). Clamping cylinders (59) are fixedly connected to the surfaces of the opposite sides of the telescopic rods (57) and the traction rod (56).

5. A temporary storage device for bovine reovirus RT-PCR test according to claim 4, characterized in that: The shaking mechanism (5) further includes vacuum suction cups (60) distributed in an annular array and fixedly connected to the inner wall of the clamping cylinder (59), an air pipe (61) is installed on the outer surface of the clamping cylinder (59), and a micro ultrasonic generator (62) is fixedly installed on the outer surface of the clamping cylinder (59).

6. The temporary storage device for bovine reovirus RT-PCR test according to claim 5, characterized in that: The outer surfaces of the upper and lower clamping cylinders (59) are fixedly connected with spiral evaporative cooling belts (63), the outer surface of one of the clamping cylinders (59) is installed with a battery (64), and the power lead of the evaporative cooling belt (63) is connected to the positive and negative electrodes of the battery (64). The outer surface of the storage tank (1) is distributed in a circular array and is provided with ventilation holes (65) extending therethrough.

7. The temporary storage device for bovine reovirus RT-PCR test according to claim 1, characterized in that: The height adjustment mechanism (7) includes a rotating screw (71) rotatably connected to the inner bottom wall of the driving cavity (12) through a bearing, the outer surface of the rotating screw (71) extends to the outer surface of the action cavity (13) and is sleeved with the internal thread of the support column (15), the outer surface of the lower end of the rotating screw (71) is fixedly sleeved with a driven gear (72), the inner bottom wall of the driving cavity (12) is fixedly connected to a rotating motor (74), the outer surface of the output shaft of the rotating motor (74) is fixedly connected to a driving gear (73) through a coupling, and the outer surface of the driving gear (73) is meshed with the outer surface of the driven gear (72).

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