An antibacterial and anti-inflammatory high-tenacity nylon suture and a method for preparing the same

Abstract

The present application relates to the field of high polymer material, and particularly relates to a preparation method of an antibacterial and anti-inflammatory high-toughness nylon suture. The specific steps are as follows: nylon single fiber is pretreated to obtain high-toughness nylon single fiber; the high-toughness nylon single fiber is sequentially soaked in an aldehyde-derivatized phenylboronic acid solution and a polyphenol solution, and then is rinsed to obtain anti-inflammatory high-toughness nylon single fiber; the anti-inflammatory high-toughness nylon single fiber is woven into nylon suture, and then is soaked in an aqueous solution of cationic high-molecular polymer and silver ion-containing compound, and is cleaned and dried to obtain antibacterial and anti-inflammatory high-toughness nylon suture. In the present application, the aldehyde-derivatized phenylboronic acid is used to modify the material, so that the phenol is better fixed on the nylon suture; the polyphenol not only has hydrogen bond interaction with the cationic high-molecular compound polymer, but also makes the silver ion reduced to nano-silver and uniformly deposited on the nylon suture. The present application overcomes the problem of single function of the existing nylon suture, and the prepared nylon suture has excellent long-acting antibacterial property and high-efficiency anti-inflammatory property.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of high polymer materials, and particularly relates to an antibacterial and anti-inflammatory high-toughness nylon suture and a preparation method thereof. BACKGROUND

[0002] In the modern medical field, when a patient has a local damage or a larger wound, the wound often needs to be sutured to avoid infection. Surgical sutures are one of the key surgical materials, which are used to connect tissues and wound tear edges to promote wound healing. However, since surgical wounds are easily attacked by bacteria and inflammation, it is necessary to develop surgical sutures with antibacterial and anti-inflammatory properties to reduce the risk of infection and improve the success rate of surgery.

[0003] Nylon, also known as polyamide, is a kind of high polymer compound containing amide bonds in the main chain. Due to its excellent mechanical properties, biocompatibility and easy handling, it is often used as a medical suture and widely used in the field of medical materials. However, the traditional nylon suture in the prior art has a single function and can only be used to suture a cracked wound. Therefore, antibacterial nylon materials are developed in large quantities, mainly by adding natural antibacterial agents, inorganic antibacterial agents or organic antibacterial agents with antibacterial properties. Antibacterial sutures can be prepared by directly dipping or coating nylon or adding antibacterial materials to the chemical fiber. However, the traditional antibacterial nylon material has difficulty in maintaining antibacterial effect during production and use, and has a single function, which limits its application in surgery.

[0004] In order to meet the requirements of scientific research and medical field for antibacterial, anti-inflammatory and high-toughness surgical sutures, and further ensure the high quality in existing uses, it is necessary to comprehensively improve it and develop a high-toughness nylon suture with good antibacterial and anti-inflammatory properties. The new type of nylon suture prepared in this aspect has a wide clinical application prospect, and has important practical significance in improving the effect of surgical operation, promoting wound healing and reducing the risk of infection. SUMMARY

[0005] In order to overcome the shortcomings of the prior art, one of the purposes of the present application is to provide a preparation method of an antibacterial and anti-inflammatory high-toughness nylon suture, which has the characteristics of simple operation and mild reaction conditions.

[0006] The second purpose of the present application is to provide an antibacterial and anti-inflammatory high-toughness nylon suture obtained by the above preparation method. The prepared nylon suture has good toughness and excellent antibacterial and anti-inflammatory effects, and can avoid postoperative infection.

[0007] One of the purposes of the present application is achieved by adopting the following technical solutions:

[0008] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0009] (1) Pre-treat nylon monofibers to obtain high-toughness nylon monofibers;

[0010] (2) After the high-toughness nylon monofiber in step (1) is modified by aldehyde-modified phenylboronic acid aqueous solution, it is soaked in polyphenol aqueous solution and rinsed with deionized water to obtain anti-inflammatory high-toughness nylon monofiber.

[0011] (3) The high-toughness nylon monofiber obtained in step (2) is woven into a high-toughness nylon suture, soaked in an aqueous solution of cationic polymer compound and silver ion compound, washed and dried to obtain an antibacterial and anti-inflammatory high-toughness nylon suture.

[0012] Further, in step (2), the aldehyde-modified phenylboronic acid is at least one of 2-aldehyde phenylboronic acid, 3-aldehyde phenylboronic acid, and 4-aldehyde phenylboronic acid.

[0013] Further, in step (2), the polyphenol is at least one of tannic acid, epigallocatechin gallate, gallic acid, and catechin.

[0014] Further, in step (3), the cationic polymeric compound is at least one of polyethyleneimine hydrochloride, polyhexyltrimethylammonium bromide, quaternized chitosan, and polyethyleneimine, and the silver-containing compound is silver chloride or silver nitrate.

[0015] Furthermore, the mass ratio of the aldehyde-modified phenylboronic acid to the high-toughness nylon monofiber is 1:10-1:100, wherein the concentration of the aldehyde-modified phenylboronic acid aqueous solution is 1g / L-10g / L, and the modification time is 6-12h; the mass ratio of the polyphenol to the high-toughness nylon monofiber is 1:20-1:200, wherein the concentration of the polyphenol aqueous solution is 0.5g / L-5g / L, and the soaking time is 1-3h.

[0016] Further, in step (3), the mass ratio of the cationic polymer compound to the anti-inflammatory high-toughness nylon suture is 1:1000-1:5000, the mass ratio of the silver ion-containing compound to the anti-inflammatory high-toughness nylon suture is 1:1000-1:5000, wherein the concentration of the cationic polymer compound in water is 0.002wt%-0.1wt%, the concentration of the silver ion-containing compound in water is 0.002wt%-0.05wt%, the soaking temperature is 20-50℃, the soaking time is 4-8h, and the fineness of the anti-inflammatory high-toughness nylon suture is 0.05mm-0.5mm.

[0017] Further, the preparation steps of the high-toughness nylon monofiber in step (1) are as follows: the nylon monofiber is rinsed with water and acetone respectively to remove impurities and dust on the surface, and then soaked in anhydrous ethanol solution of Lewis acid. After soaking, it is rinsed clean.

[0018] Further, in step (1), the Lewis acid is at least one of calcium chloride, aluminum chloride, zinc chloride, and boron fluoride, and the concentration of the Lewis acid in the anhydrous ethanol solution is 0.5wt%-10wt%.

[0019] Furthermore, the nylon monofiber is an amino-terminated nylon 66 monofiber obtained by meltblowing, with a fineness of 0.5-10 dtex, an immersion temperature of 40-70℃, and a immersion time of 4-8 hours.

[0020] The second objective of this invention is achieved by the following technical solution:

[0021] An antibacterial, anti-inflammatory, and high-toughness nylon suture was prepared by the above method.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] 1. This invention provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon sutures. By modifying amino-terminated nylon 66 monofibrils with aldehyde-based phenylboronic acid, and using aldehyde-based phenylboronic acid as a bridge, polyphenolic compounds are introduced onto the surface of the nylon monofibrils, thereby improving their surface activity. Furthermore, the introduction of polyphenolic groups not only allows for hydrogen bond interactions with cationic polymers but also enables redox reactions with silver ions, allowing for the uniform deposition of an antibacterial coating containing nano-silver on the surface of the nylon suture.

[0024] 2. This invention also provides an antibacterial, anti-inflammatory, and high-toughness nylon suture prepared by the above-described method. The antibacterial, anti-inflammatory, and high-toughness nylon suture prepared by this invention exhibits long-lasting antibacterial properties. It continuously releases nano-silver particles on the surface of the nylon suture, forming an antibacterial protective layer that effectively inhibits the growth of various pathogenic bacteria, including drug-resistant strains, thus possessing multiple antibacterial properties. Simultaneously, the anti-inflammatory effect of silver ions can reduce the inflammatory response of tissues surrounding the wound, promoting the speed and quality of wound healing. Therefore, the nylon suture prepared by this invention achieves both long-lasting antibacterial and good anti-inflammatory effects. The antibacterial, anti-inflammatory, and high-toughness nylon suture obtained by this invention combines high toughness, long-lasting antibacterial, and anti-inflammatory effects. When used as a surgical suture, it can significantly reduce the risk of wound infection, promote wound healing, and improve the success rate of surgery. Attached Figure Description

[0025] Figure 1 The concentration of TNF-α in the supernatant of the samples obtained in Examples 1-5 and Comparative Examples 1-8 of this invention after co-culturing with macrophages for 3 days is shown. Detailed Implementation

[0026] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0027] Example 1

[0028] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0029] (1) A amino-terminated nylon 66 monofibrils with a fineness of 0.5 dtex were prepared by melt-blowing. They were cleaned with water and acetone to remove impurities and dust from the surface. Then they were soaked in an anhydrous ethanol solution of calcium chloride with a mass concentration of 0.5 wt% for 8 hours at 40°C. After washing with deionized water, high-toughness nylon monofibrils were obtained.

[0030] (2) Dissolve 10g of 2-aldehyde phenylboronic acid in 1L of deionized water to prepare a 2-aldehyde phenylboronic acid solution. Take 100g of the high-toughness nylon monofiber obtained in step (1) and soak it in the above solution. After 6 hours, wash it clean. Dissolve 5g of tannic acid in 1L of deionized water to prepare a polyphenol solution. Take the cleaned high-toughness nylon monofiber and soak it in the above polyphenol solution. After 3 hours, wash it clean to obtain anti-inflammatory high-toughness nylon monofiber.

[0031] (3) The above-obtained anti-inflammatory and high-toughness nylon monofibers are woven into anti-inflammatory and high-toughness nylon sutures with a fineness of 0.05 mm. 100 g of the sutures are then soaked in a 1 L mixed aqueous solution containing 0.1 wt% polyethyleneimine hydrochloride and 0.002 wt% silver chloride. After soaking at 50 °C for 4 h, the free cationic polymer compounds and silver nanoparticles are washed away. After drying, the antibacterial and anti-inflammatory high-toughness nylon sutures are obtained.

[0032] Example 2

[0033] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0034] (1) A amino-terminated nylon 66 monofibrils with a fineness of 5 fen tex were prepared by melt-blowing method. They were cleaned with water and acetone to remove impurities and dust from the surface. Then they were soaked in anhydrous ethanol solution of aluminum chloride with a mass concentration of 5 wt% at 70°C for 4 h. After soaking, they were washed with deionized water to obtain high-toughness nylon monofibrils.

[0035] (2) Dissolve 1g of 3-aldehyde phenylboronic acid in 1L of deionized water to prepare a 3-aldehyde phenylboronic acid solution. Take 100g of the high-toughness nylon monofibrate obtained in step (1) and soak it in the above solution. After 12 hours, wash it clean. Dissolve 0.5g of epigallocatechin gallate in 1L of deionized water to prepare a polyphenol solution. Take the cleaned high-toughness nylon monofibrate and soak it in the above polyphenol solution. After 3 hours, wash it clean to obtain anti-inflammatory high-toughness nylon monofibrate.

[0036] (3) The anti-inflammatory and high-toughness nylon monofibrils obtained above are woven into anti-inflammatory and high-toughness nylon sutures with a fineness of 0.2 mm. 100 g of the sutures are then soaked in a 1 L mixed aqueous solution containing 0.002 wt% polyhexadecyltrimethylammonium bromide and 0.01 wt% silver chloride. After soaking at 20 °C for 8 h, the free cationic polymer compounds and silver nanoparticles are washed away. After drying, the antibacterial and anti-inflammatory high-toughness nylon sutures are obtained.

[0037] Example 3

[0038] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0039] (1) A amino-terminated nylon 66 monofibrils with a fineness of 10 dtex were prepared by melt-blowing method. They were cleaned with water and acetone to remove impurities and dust from the surface. Then they were soaked in an anhydrous ethanol solution of zinc chloride with a mass concentration of 10 wt% for 4 h at 70 °C. After being washed with deionized water, high-toughness nylon monofibrils were obtained.

[0040] (2) Dissolve 5g of 4-aldehyde phenylboronic acid in 1L of deionized water to prepare a 4-aldehyde phenylboronic acid solution. Take 100g of the high-toughness nylon monofiber obtained in step (1) and soak it in the above solution. After 8 hours, wash it clean. Dissolve 2.5g of gallic acid in 1L of deionized water to prepare a polyphenol solution. Take the cleaned high-toughness nylon monofiber and soak it in the above polyphenol solution. After 1 hour, wash it clean to obtain anti-inflammatory high-toughness nylon monofiber.

[0041] (3) The anti-inflammatory and high-toughness nylon monofibrils obtained above are woven into anti-inflammatory and high-toughness nylon sutures with a fineness of 0.5 mm. 100 g of the sutures are then soaked in a 1 L mixed aqueous solution containing 0.005 wt% quaternized chitosan and 0.005 wt% silver chloride. After soaking at 40 °C for 6 h, the free cationic polymer compounds and silver nanoparticles are washed away. After drying, the antibacterial and anti-inflammatory high-toughness nylon sutures are obtained.

[0042] Example 4

[0043] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0044] (1) A amino-terminated nylon 66 monofibrils with a fineness of 5 fen tex were prepared by melt-blowing method. They were cleaned with water and acetone to remove impurities and dust from the surface. Then they were soaked in an anhydrous ethanol solution of boron trifluoride with a mass concentration of 5 wt% for 6 h at 50 °C. After being washed with deionized water, high-toughness nylon monofibrils were obtained.

[0045] (2) Dissolve 2.5g of 4-aldehyde phenylboronic acid and 2.5g of 2-aldehyde phenylboronic acid in 1L of deionized water to prepare a mixed solution of aldehyde phenylboronic acid. Take 100g of the high-toughness nylon monofiber obtained in step (1) and soak it in the above solution. After 12 hours, wash it clean. Dissolve 1g of catechin in 1L of deionized water to prepare a polyphenol solution. Take the cleaned high-toughness nylon monofiber and soak it in the above polyphenol solution. After 3 hours, wash it clean to obtain anti-inflammatory high-toughness nylon monofiber.

[0046] (3) The above-obtained anti-inflammatory and high-toughness nylon monofibers are woven into anti-inflammatory and high-toughness nylon sutures with a fineness of 0.1 mm. 100 g of the sutures are then soaked in a mixed aqueous solution containing 0.005 wt% polyethyleneimine and 0.005 wt% silver chloride. After soaking at 50 °C for 6 h, the free cationic polymer compounds and silver nanoparticles are washed away. After drying, the antibacterial, anti-inflammatory, and high-toughness nylon sutures are obtained.

[0047] Example 5

[0048] A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture includes the following steps:

[0049] (1) A amino-terminated nylon 66 monofibrils with a fineness of 5 fen tex were prepared by melt-blowing method. They were cleaned with water and acetone to remove impurities and dust from the surface. Then, they were soaked in an anhydrous ethanol solution of calcium chloride and aluminum chloride with a mass concentration of 2.5 wt% and 2.5 wt% respectively. After soaking at 70°C for 6 h, they were washed with deionized water to obtain high-toughness nylon monofibrils.

[0050] (2) Prepare an aldehyde-phenylboronic acid mixed solution by dissolving 5g of 2-aldehyde-phenylboronic acid and 2.5g of 3-aldehyde-phenylboronic acid in 1L of deionized water. Take 100g of the high-toughness nylon monofiber obtained in step (1) and soak it in the above solution. After 6 hours, wash it clean. Prepare a polyphenol solution by dissolving 2.5g of tannic acid and 2.5g of catechin in 1L of deionized water. Soak the cleaned high-toughness nylon monofiber in the above polyphenol solution. After 3 hours, wash it clean to obtain anti-inflammatory high-toughness nylon monofiber.

[0051] (3) The anti-inflammatory and high-toughness nylon monofibers obtained above are woven into anti-inflammatory and high-toughness nylon sutures with a fineness of 0.5 mm. 100 g of the sutures are soaked in a mixed aqueous solution containing 0.005 wt% quaternized chitosan, 0.005 wt% polyethyleneimine, and 0.005 wt% silver chloride. After soaking at 50 °C for 6 h, the free cationic polymer compounds and silver nanoparticles are washed away. After drying, the antibacterial and anti-inflammatory high-toughness nylon sutures are obtained.

[0052] Comparative Example 1

[0053] Comparative Example 1 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon sutures. The difference between Comparative Example 1 and Example 2 is that the step of soaking in 0.5 wt% calcium chloride in anhydrous ethanol solution in step (1) is omitted. Otherwise, it is the same as Example 1.

[0054] Comparative Example 2

[0055] Comparative Example 2 provides a method for preparing antibacterial and anti-inflammatory high-toughness nylon sutures. The difference from Example 1 is that the operation in step (2) is omitted. That is, after the high-toughness nylon monofiber obtained in step (1) is woven into a high-toughness nylon suture with a fineness of 0.05 mm, 100 g of the above high-toughness nylon suture is soaked in a mixed aqueous solution containing 0.01 wt% polyethyleneimine hydrochloride and 0.002 wt% silver chloride. After reacting at 50°C for 4 h, the free cationic polymer compounds and silver nanoparticles are washed off, and then dried.

[0056] Comparative Example 3

[0057] Comparative Example 3 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon sutures. The difference from Example 1 is that the anti-inflammatory and high-toughness nylon monofibers obtained in step (2) are woven into nylon sutures with a fineness of 0.05 mm, and then placed in deionized water, soaked at 50°C for 4 hours, and then dried.

[0058] Comparative Example 4

[0059] Comparative Example 4 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon sutures. The difference from Example 1 is that the soaking treatment of 2-aldehyde phenylboronic acid is omitted. Instead, 5g of tannic acid is dissolved in 1L of deionized water to prepare a polyphenol solution. 100g of the high-toughness nylon monofiber obtained in step (1) is soaked in the above polyphenol solution. After 3 hours, it is cleaned. The subsequent operations are the same as in Example 1.

[0060] Comparative Example 5

[0061] Comparative Example 5 provides a method for preparing antibacterial and anti-inflammatory high-toughness nylon sutures. The difference from Example 1 is that step (2) is as follows: First, the high-toughness nylon monofibers obtained in step (1) are braided into 0.5mm high-toughness nylon sutures. Then, 100g of the high-toughness nylon sutures are soaked in 1L of a mixed aqueous solution of 0.05wt% polyethyleneimine hydrochloride and 0.05wt% silver chloride. After reacting at 50℃ for 6h, the free cationic polymer compounds and nano-silver particles are washed away, and the sutures are dried to obtain antibacterial high-toughness nylon sutures.

[0062] (3) Dissolve 10g of 2-aldehyde phenylboronic acid in 1L of deionized water to prepare a 2-aldehyde phenylboronic acid solution. Take 100g of the antibacterial high-toughness nylon suture obtained in step (2) and soak it in the above solution. After 6 hours, clean it. Dissolve 5g of tannic acid in 1L of deionized water to prepare a polyphenol solution. Take the cleaned high-toughness nylon monofiber and soak it in the polyphenol solution. After 3 hours, clean it to obtain the nylon suture.

[0063] Comparative Example 6

[0064] Comparative Example 6 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon monofiber. The difference between this method and Example 1 is that tannic acid is replaced with 6-guanidinoacetic acid, while the rest is the same as Example 1.

[0065] Comparative Example 7

[0066] Comparative Example 7 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon monofiber. The difference from Example 1 is that polyethyleneimine hydrochloride is omitted, and the mass of silver chloride in the solution is adjusted to the sum of the masses of polyethyleneimine hydrochloride and silver chloride in Example 1. Everything else is the same as Example 1.

[0067] Comparative Example 8

[0068] Comparative Example 8 provides a method for preparing antibacterial, anti-inflammatory, and high-toughness nylon monofiber. The difference from Example 1 is that silver chloride is omitted, and the mass of polyethyleneimine hydrochloride in the solution is adjusted to the sum of the masses of polyethyleneimine hydrochloride and silver chloride in Example 1. Everything else is the same as Example 1.

[0069] Experimental Example 1

[0070] 1. Mechanical property testing

[0071] Three 10cm long monofilament fibers were taken from the antibacterial, anti-inflammatory, and high-toughness nylon sutures of Examples 1-5 and Comparative Examples 1-8, respectively. One end of the nylon monofilament was fixed to a clamp on one side, ensuring that the fiber would not be damaged when clamped. Then, the other end of the nylon fiber was connected to a force gauge, ensuring that the connection was firm and that there was no bending between the fiber and the force gauge. The force gauge was started and stretched at a rate of 2mm / min. The stretching curve was recorded, and the elongation at break of the monofilament was calculated. The results are shown in Table 1.

[0072] Table 1

[0073] Test sample Elongation at break / % Example 1 18.54±2.38 Example 2 19.49±3.21 Example 3 17.37±1.39 Example 4 21.37±4.96 Example 5 20.09±2.98 Comparative Example 1 12.97±1.59 Comparative Example 2 18.77±2.13 Comparative Example 3 19.01±2.11 Comparative Example 4 18.98±3.12 Comparative Example 5 17.68±1.25 Comparative Example 6 20.33±3.56 Comparative Example 7 19.31±2.73 Comparative Example 8 18.15±1.96

[0074] As shown in Table 1, the nylon sutures prepared in the embodiments and comparative examples of the present invention all exhibit good elongation at break after being soaked in anhydrous ethanol solution of Lewis acid. Among them, Comparative Example 1, which was not treated in this way, has an elongation at break of only 12.97±1.59%, indicating that soaking the nylon sutures in ethanol solution of Lewis acid can effectively improve their elongation at break.

[0075] 2. Antibacterial test

[0076] Take 1g of the nylon sutures obtained in Examples 1-5 and Comparative Examples 1-8 respectively and place them in sterile conical flasks. Then add 50mL of 0.03mol / L phosphate buffer and 5mL of 10 8 A bacterial suspension of Staphylococcus aureus or Escherichia coli at CFU / mL was incubated at 37°C for 12 h and 24 h after the liquid had seeped into the culture medium, and then colony counts were performed.

[0077] The formula for calculating the antibacterial rate is: X = (AB) / A × 100%, where X is the antibacterial rate, A is the average number of colonies before shaking the sample, and B is the average number of colonies after shaking the sample. The antibacterial rates of the nylon sutures prepared in Examples 1-5 and Comparative Examples 1-8 against Staphylococcus aureus and Escherichia coli are shown in Tables 2 and 3, respectively.

[0078] Table 2

[0079]

[0080]

[0081] Table 3

[0082] Test sample 12h 24h Example 1 83％ 95％ Example 2 86％ 96％ Example 3 90％ 99％ Example 4 88％ 98％ Example 5 91％ 100％ Comparative Example 1 84％ 92％ Comparative Example 2 0％ 0％ Comparative Example 3 0％ 0％ Comparative Example 4 0％ 0％ Comparative Example 5 15％ 3％ Comparative Example 6 0％ 0％ Comparative Example 7 37％ 19％ Comparative Example 8 26％ 11％

[0083] As shown in Tables 2 and 3, the antibacterial and anti-inflammatory surgical sutures prepared by the present invention have good antibacterial properties. The nylon sutures prepared in Examples 1-5 all have good antibacterial effects. After 24 hours of antibacterial performance testing, it can be seen that the antibacterial rate has been significantly improved, and the antibacterial rate of each sample is above 90%, which proves that the nylon sutures prepared in Examples 1-5 of the present invention have good long-lasting antibacterial properties.

[0084] Comparative Example 2 omitted the soaking treatment with 2-aldehyde phenylboronic acid and polyphenol solution, and Comparative Example 4 omitted the soaking treatment with 2-aldehyde phenylboronic acid solution. Neither of these nylon sutures had polyphenol fixation on their surface, and after 24 hours of treatment, their antibacterial rate was 0%, failing to achieve a long-lasting antibacterial effect. Comparative Example 3 omitted the soaking with polyethyleneimine hydrochloride and silver chloride, directly soaking the nylon sutures in 2-aldehyde phenylboronic acid solution and tannic acid solution. Comparative Example 5 first soaked the nylon sutures in polyethyleneimine hydrochloride and silver chloride solution, then soaked them in 2-aldehyde phenylboronic acid and tannic acid solution. Comparative Example 6 replaced the polyphenol with 6-guanidinoacetic acid solution to obtain the nylon sutures. The results showed that its antibacterial rate was far lower than that of the antibacterial, anti-inflammatory, and high-toughness nylon sutures obtained in Examples 1-5. Therefore, the above experimental results indicate that the simultaneous action of aldehyde-modified phenylboronic acid and polyphenol, as well as the order of addition of aldehyde-modified phenylboronic acid, significantly affect the long-lasting antibacterial properties of nylon sutures. It is evident that the modification treatment of aldehyde-modified phenylboronic acid and the immersion treatment of polyphenols are prerequisites for the nylon sutures to obtain long-lasting antibacterial properties.

[0085] Comparative Example 7 omitted the soaking treatment with polyethyleneimine hydrochloride and increased the amount of silver chloride. Comparative Example 8 omitted the soaking treatment with silver chloride and increased the amount of polyethyleneimine hydrochloride. As shown in Tables 2 and 3, after 24 hours of antibacterial treatment, the antibacterial rate of the nylon sutures obtained in Comparative Examples 7 and 8 was only about 30%, and their antibacterial effect was far inferior to that of Examples 1-5.

[0086] Therefore, it can be seen that polyethyleneimine hydrochloride and silver chloride solution can work together to enhance the antibacterial properties of nylon sutures. In summary, under the premise of simultaneous application of aldehyde-modified phenylboronic acid and polyphenol aqueous solution, the combined effect of cationic polymers and silver-containing particles can give the prepared nylon sutures excellent multiple antibacterial properties.

[0087] 3. Anti-inflammatory effect

[0088] Prepare mouse-derived macrophages and then induce them to the M1 phenotype (inflammatory phenotype) at a concentration of 2 × 10⁻⁶. 5The sutures were laid in 24-well plates at a density equal to the number of wells. Then, 0.05g of sutures prepared in Examples 1-5 and Comparative Examples 1-8 were sterilized and cut into 0.5cm segments. These segments were placed in Transwell permeable cell culture chambers and then in the well plates. After 3 days of culture, the concentration of TNF-α in the supernatant was measured using an ELISA kit. The results are shown below. Figure 1 .

[0089] TNF-α is a typical inflammation-related factor. As can be seen from the figure, the concentrations of TNF-α in Examples 1-5 are relatively low, indicating that the prepared nylon sutures have better anti-inflammatory effects. Therefore, the nylon sutures prepared in Examples 1-5 have better anti-inflammatory properties.

[0090] In summary, the antibacterial, anti-inflammatory, and high-toughness nylon suture provided in this application utilizes aldehyde-modified phenylboronic acid as a bridging agent during its preparation process to fix polyphenols onto the surface of nylon monofibrils. This allows for hydrogen bonding interactions between the polyphenol groups and the amino groups in the cationic polymer compound, as well as redox interactions between the polyphenols and silver ions. These interactions form a uniform nano-silver antibacterial coating on the surface of the nylon suture, continuously releasing nano-silver particles to form an antibacterial protective layer. This effectively kills and inhibits the growth of various pathogenic bacteria, including drug-resistant strains, exhibiting multiple antibacterial properties. Simultaneously, the anti-inflammatory effect of silver ions can reduce the inflammatory response of tissues surrounding the wound, promoting the speed and quality of wound healing. Therefore, the nylon suture prepared by this invention has better long-lasting antibacterial and highly effective anti-inflammatory effects.

[0091] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture, characterized in that, Includes the following steps: (1) Pre-treat nylon monofibers to obtain high-toughness nylon monofibers; (2) After the high-toughness nylon monofiber in step (1) is modified by aldehyde-modified phenylboronic acid aqueous solution, it is soaked in polyphenol aqueous solution and rinsed with deionized water to obtain anti-inflammatory high-toughness nylon monofiber. (3) The anti-inflammatory and high-toughness nylon monofiber obtained in step (2) is woven into anti-inflammatory and high-toughness nylon suture, soaked in an aqueous solution of cationic polymer compound and silver ion compound, washed and dried to obtain antibacterial and anti-inflammatory high-toughness nylon suture; The nylon monofiber is an amino-terminated nylon 66 monofiber obtained by meltblowing, with a fineness of 0.5-10 dtex.

2. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, In step (2), the aldehyde-modified phenylboronic acid is at least one of 2-aldehyde phenylboronic acid, 3-aldehyde phenylboronic acid, and 4-aldehyde phenylboronic acid.

3. The method for preparing an antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, In step (2), the polyphenol is at least one of tannic acid, epigallocatechin gallate, gallic acid, and catechin.

4. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, In step (3), the cationic polymer compound is at least one of polyethyleneimine hydrochloride, quaternized chitosan, and polyethyleneimine, and the silver ion-containing compound is silver chloride or silver nitrate.

5. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, The mass ratio of aldehyde-modified phenylboronic acid to high-toughness nylon monofibrate is 1:10-1:100, wherein the concentration of the aqueous solution of aldehyde-modified phenylboronic acid is 1g / L-10g / L, and the modification time is 6-12h. The mass ratio of polyphenol to high-toughness nylon monofibrate is 1:20-1:200, wherein the concentration of the aqueous solution of polyphenol is 0.5g / L-5g / L, and the soaking time is 1-3h.

6. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, In step (3), the mass ratio of the cationic polymer compound to the anti-inflammatory high-toughness nylon suture is 1:1000-1:5000, the mass ratio of the silver ion-containing compound to the anti-inflammatory high-toughness nylon suture is 1:1000-1:5000, the concentration of the cationic polymer compound in water is 0.002wt%-0.1wt%, the concentration of the silver ion-containing compound in water is 0.002wt%-0.05wt%, the soaking temperature is 20-50℃, the soaking time is 4-8h, and the fineness of the anti-inflammatory high-toughness nylon suture is 0.05mm-0.5mm.

7. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 1, characterized in that, The preparation steps of high-toughness nylon monofiber in step (1) are as follows: rinse the nylon monofiber with water and acetone respectively to remove impurities and dust on the surface, then soak it in anhydrous ethanol solution of Lewis acid, and rinse it clean after soaking.

8. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 7, characterized in that, In step (1), the Lewis acid is at least one of calcium chloride, aluminum chloride, zinc chloride, and boron fluoride, and the concentration of the Lewis acid in the anhydrous ethanol solution is 0.5wt%-10wt%.

9. The method for preparing the antibacterial, anti-inflammatory, and high-toughness nylon suture according to claim 7, characterized in that, Soaking temperature is 40-70℃, time is 4-8 hours.

10. The antibacterial, anti-inflammatory, and high-toughness nylon suture obtained by the preparation method according to any one of claims 1-9.

Images