A cooling device and a gas collection method for collecting soil respiration gas

Abstract

This invention discloses a cooling device and method for collecting soil respiration gases. The device includes a motor, a water pump, a breathing ring, a water pipe, a water bucket, a switch, an external power cord, a water inlet, and a water outlet. The motor and water pump are assembled together in a sheet metal assembly box. The motor is fixed to the cover on the top surface of the sheet metal assembly box, and the water pump is fixed to the base of the sheet metal assembly box. The motor and water pump are connected by an electric wire. The switch and external power cord are installed at the rear of the sheet metal assembly box. A water inlet is set on the side of the sheet metal assembly box near the water pump, and a water outlet is set at the front of the sheet metal assembly box. The water outlet is connected to the breathing ring through a water pipe, and the breathing ring is connected to the water bucket through a water pipe. Water in the water bucket is connected to the water inlet of the sheet metal assembly box through a water pipe. Ice blocks are placed in the water bucket. This invention solves the problem that the temperature in the breathing chamber gradually increases with the increase of the sealing time, thereby affecting the rate of soil respiration and carbon emissions.

Description

Technical Field

[0001] This invention relates to the field of soil respiration gas collection, and in particular to a cooling device and a method for collecting soil respiration gases. Background Technology

[0002] In forest ecosystems, soil respiration is the process of CO2 exchange between soil and the atmosphere, a crucial component of the carbon cycle and carbon transport, and its dynamic changes significantly impact carbon cycle research. Therefore, collecting and measuring CO2 released during soil respiration is particularly important and critical for studying ecosystem carbon cycles. However, collecting and measuring CO2 released during soil respiration is often difficult. Previous studies have frequently used NaOH solution for collection, but this method often encounters problems. Since the NaOH solution is placed in a sealed cylindrical respiration chamber, the temperature inside the chamber gradually increases with the duration of closure, affecting the soil respiration rate and thus CO2 emission and collection. Therefore, cooling the cylindrical respiration chamber is essential during the collection of CO2 released through soil respiration.

[0003] Based on this, the present invention adds a water circulation device to the outside of the cylindrical ring, which continuously supplies cooling water to the breathing chamber to cool it down, effectively controlling the temperature of the breathing chamber and ensuring that the CO2 released by soil respiration is not affected by the temperature rise. Summary of the Invention

[0004] To address the problems existing in the prior art, the present invention provides a cooling device and a method for collecting soil respiration gases, which solves the problem that the temperature in the respiration chamber gradually increases with the increase of the sealing time, thereby affecting the rate of soil respiration and thus affecting the release of CO2.

[0005] The specific technical solution of the present invention is as follows:

[0006] A cooling device for collecting soil respiration gases includes a motor, a water pump, a breathing ring, a water pipe, a water tank, a switch, an external power cord, a water inlet, and a water outlet.

[0007] The motor and water pump are assembled together in a sheet metal assembly box. The motor is fixed to the cover on the top of the sheet metal assembly box, and the water pump is fixed to the base of the sheet metal assembly box. The motor and water pump are connected by wires. The switch and external power cord are installed at the back of the sheet metal assembly box. A water inlet is set on the side of the sheet metal assembly box near the water pump, and a water outlet is set at the front of the sheet metal assembly box. The water outlet is connected to a breather ring through a water pipe. The breather ring is connected to a water bucket through a water pipe. The water bucket is connected to the water inlet of the sheet metal assembly box through a water pipe. Ice is placed in the water bucket.

[0008] Preferably, the motor and the water pump are connected by an electric wire to supply power to the water pump.

[0009] Preferably, the water outlet includes a first water outlet, a second water outlet, a third water outlet, a fourth water outlet, and a fifth water outlet;

[0010] The breathing rings include the first breathing ring, the second breathing ring, the third breathing ring, the fourth breathing ring, the fifth breathing ring, the sixth breathing ring, and the seventh breathing ring;

[0011] The fifth outlet is connected to the first breathing ring, the first breathing ring is connected to the second breathing ring, the second breathing ring is connected to the third breathing ring, and the third breathing ring is connected to the water bucket.

[0012] The fourth outlet connects to the fourth breathing ring, the fourth breathing ring connects to the fifth breathing ring, and the fifth breathing ring connects to the water bucket.

[0013] The third outlet connects to the sixth breathing ring, the sixth breathing ring connects to the seventh breathing ring, and the seventh breathing ring connects to the water bucket.

[0014] The second and first water outlets should be plugged when not in use.

[0015] Preferably, the first breathing ring includes an inner tube, an outer tube, a breathing ring inlet and a breathing ring outlet, a beaker, and a rubber cap;

[0016] A beaker is placed inside an inner tube, which is placed inside an outer tube. The inner and outer tubes are spaced 1 cm apart, with their tops flush. The bottom of the inner tube extends 3-5 cm beyond the outer tube. The outer and inner tubes are sealed with a circular ring, and the joint is sealed with sealant. The space between the outer and inner tubes is for water circulation. The inlet of the breathing ring is located on one side of the outer tube, near its top surface, and the outlet of the breathing ring is located on the other side of the outer tube, below the height of the inlet. A rubber cap is placed on the top of the first breathing ring to seal it when collecting soil respiration gases. NaOH solution is placed inside the beaker.

[0017] The first, second, third, fourth, fifth, sixth, and seventh respiratory rings have the same structure.

[0018] Both the inner and outer tubes are PVC pipes; the outer tube has a diameter 2cm larger than the inner tube.

[0019] Preferably, the water pipe is a high-temperature resistant silicone hose, which connects the water inlet, water pump, water outlet and breather ring to allow water to circulate throughout the device.

[0020] Preferably, the water bucket is used to hold water, one end of the water outlet of the breathing ring is connected to a water pipe, and the water pipe of the water outlet is placed in the water bucket to collect the circulating water; the water inlet is connected to a water pipe, and the water pipe is placed in the water bucket to supply water to the circulation device.

[0021] Preferably, a method for collecting respiration gases from soil using a cooling device includes the following steps:

[0022] Step S1: According to the sampling requirements, set up the breathing ring and insert the breathing ring into the soil of the sampling area. The insertion depth is the length of the inner tube that is longer than the outer tube.

[0023] Step S2: Place a small beaker containing NaOH solution in each breathing ring, cover it with a rubber cap to seal it, and connect the water pipe to the inlet and outlet of each breathing ring to make the entire water circulation connected.

[0024] Step S3: Fill the bucket with water, put the other end of the water pipe inserted into the water pump inlet into the bucket, and put the last end of the breathing ring outlet into the bucket. This satisfies the water intake and ensures that the water at the outlet is not wasted, so that the water supply can continue to be circulated.

[0025] Step S4: Use a thermometer to measure the temperature of the water in the bucket and determine whether the water temperature is the same as the ambient air temperature. If they are the same, proceed to step S5; if they are not the same, add ice cubes to cool the water in the bucket.

[0026] Step S5: After all preparations are complete, plug in the power, turn on the switch, and the water pump will start pumping water to circulate the entire water system and ensure that the temperature inside the breathing ring does not rise as the placement time increases.

[0027] Step S6: After collecting the gas according to the sampling time designed in the experiment, turn off the power, open the rubber cap on the breathing ring, and take out the beaker for subsequent measurement of the amount of CO2 in soil respiration.

[0028] The beneficial effects of the device and method for collecting soil respiration gases of the present invention are as follows:

[0029] 1. This invention can effectively control the temperature inside the breathing ring, ensuring that the soil temperature is basically the same as the air temperature, so that the gas released by the soil respiration will not be affected by the temperature rise.

[0030] 2. This invention can connect multiple breathing rings simultaneously and cool them down at the same time.

[0031] 3. This invention allows for the addition of appropriate ice cubes based on weather changes, facilitating control of the inlet water temperature. Simultaneously, by monitoring the outlet water temperature, changes in the breathing chamber temperature can be monitored, allowing for adjustments to the amount of ice in the bucket.

[0032] 4. In this invention, the water inlet pipe and the water outlet pipe are placed in the same water bucket, allowing the water in the bucket to be reused and the water supply to be circulated, thus saving water. Attached Figure Description

[0033] Figure 1 Diagram of an apparatus for collecting respiration gases from soil.

[0034] Figure 2 This is a structural diagram of the first respiratory ring.

[0035] Attached diagram labels: 1-Motor, 2-Water pump, 3-Breathing ring, 4-Water pipe, 5-Water bucket, 6-Switch, 7-External power cord, 8-Inlet, 9-Outlet, 3-1-First breathing ring, 3-2-Second breathing ring, 3-3-Third breathing ring, 3-4-Fourth breathing ring, 3-5-Fifth breathing ring, 3-6-Sixth breathing ring, 3-7-Seventh breathing ring, 9-1-First outlet, 9-2-Second outlet, 9-3-Third outlet, 9-4-Fourth outlet, 9-5-Fifth outlet, 3-1-1-Inner tube, 3-1-2-Outer tube, 3-1-3-Breathing ring inlet, 3-1-4-Breathing ring outlet, 3-1-5-Beaker, 3-1-6-Rubber lid, 10-Ice cube. Detailed Implementation

[0036] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0037] The specific embodiments of the present invention are described below to enable those skilled in the art to understand the present invention. However, it should be understood that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, various changes are obvious as long as they are within the spirit and scope of the present invention as defined and determined by the appended claims. All inventions utilizing the concept of the present invention are protected.

[0038] like Figure 1 As shown, a cooling device for collecting soil respiration gases includes a motor 1, a water pump 2, a breathing ring 3, a water pipe 4, a water bucket 5, a switch 6, an external power cord 7, a water inlet 8, a water outlet 9, and ice blocks 10.

[0039] Motor 1 and water pump 2 are assembled together in a sheet metal assembly box. Motor 1 is fixed to the cover on the top of the sheet metal assembly box, and water pump 2 is fixed to the base of the sheet metal assembly box. Motor 1 and water pump 2 are connected by wires. Switch 6 and external power cord 7 are installed at the back of the sheet metal assembly box. A water inlet 8 is set on the side of the sheet metal assembly box near the water pump. A water outlet 9 is set at the front of the sheet metal assembly box. The water outlet 9 is connected to the breathing ring 3 through a water pipe 4. The breathing ring 3 is connected to the water bucket through a water pipe 4. The water bucket is connected to the water inlet 8 of the sheet metal assembly box through a water pipe 4. Ice cubes 10 are placed in the water bucket 5.

[0040] In this embodiment, the motor 1 and the water pump 2 are connected by an electric wire to supply power to the water pump 2.

[0041] The water outlets in this implementation plan include the first water outlet 9-1, the second water outlet 9-2, the third water outlet 9-3, the fourth water outlet 9-4, and the fifth water outlet 9-5;

[0042] The breathing ring 3 includes a first breathing ring 3-1, a second breathing ring 3-2, a third breathing ring 3-3, a fourth breathing ring 3-4, a fifth breathing ring 3-5, a sixth breathing ring 3-6, and a seventh breathing ring 3-7;

[0043] The fifth outlet 9-5 is connected to the first breathing ring 3-1, the first breathing ring 3-1 is connected to the second breathing ring 3-2, the second breathing ring 3-2 is connected to the third breathing ring 3-3, and the third breathing ring 3-3 is connected to the water bucket 5;

[0044] The fourth outlet 9-4 is connected to the fourth breathing ring 3-4, the fourth breathing ring 3-4 is connected to the fifth breathing ring 3-5, and the fifth breathing ring 3-5 is connected to the water tank 5;

[0045] The third outlet 9-3 connects to the sixth breathing ring 3-6, the sixth breathing ring 3-6 connects to the seventh breathing ring 3-7, and the seventh breathing ring 3-7 connects to the water bucket 5.

[0046] The first breathing ring 3-1 in this embodiment includes an inner tube 3-1-1, an outer tube 3-1-2, a breathing ring inlet 3-1-3 and a breathing ring outlet 3-1-4, a beaker 3-1-5 and a rubber cap 3-1-6;

[0047] Beaker 3-1-5 is placed inside inner tube 3-1-1, which is placed inside outer tube 3-1-2. There is a 1cm gap between inner tube 3-1-1 and outer tube 3-1-2. The tops of inner tube 3-1-1 and outer tube 3-1-2 are flush, while the bottom of inner tube 3-1-1 extends 3-5cm beyond outer tube 3-1-2. A circular ring seals the connection between outer tube 3-1-2 and inner tube 3-1-1, and the joint is sealed with sealant. The space between the inner tube 3-1-1 and the outer tube 3-1-2 is for water circulation. The inlet 3-1-3 of the breathing ring is located on one side of the outer tube 3-1-2 and close to the top surface of the outer tube 3-1-2. The outlet 3-1-4 of the breathing ring is located on the other side of the outer tube 3-1-2 and below the inlet 3-1-3 of the breathing ring. The rubber cap 3-1-6 is located at the top of the first breathing ring 3-1 and is used to cover and seal the area when collecting soil breathing gases. NaOH solution is placed inside the beaker.

[0048] The first breathing ring 3-1, the second breathing ring 3-2, the third breathing ring 3-3, the fourth breathing ring 3-4, the fifth breathing ring 3-5, the sixth breathing ring 3-6, and the seventh breathing ring 3-7 have the same structure.

[0049] In this implementation scheme, both the inner tube 3-1-1 and the outer tube 3-1-2 are PVC pipes; the diameter of the outer tube 3-1-2 is 2cm larger than that of the inner tube.

[0050] In this embodiment, the water pipe 4 is a high-temperature resistant silicone hose, which connects the water inlet 8, water pump 2, water outlet 9 and breather ring 3 to allow water to circulate throughout the device.

[0051] In this embodiment, the water bucket 5 is used to hold water. One end of the breathing ring outlet 3-1-9 is connected to the water pipe 4, which is placed in the water bucket 5 to collect the circulating water. The water inlet 8 is connected to the water pipe 4, which is placed in the water bucket to supply water to the circulation device.

[0052] When implemented, this embodiment includes a cooling device for collecting soil breathing gases, comprising a motor 1, a water pump 2, a breathing ring 3, a water pipe 4, a water bucket 5, a switch 6, an external power cord 7, a water inlet 8, a water outlet 9, and ice blocks 10.

[0053] Motor 1 and water pump 2 are assembled together in a sheet metal assembly box. Motor 1 is fixed to the cover on top of the assembly box, and water pump 2 is fixed to the base of the assembly box. An electrical wire connects motor 1 and water pump 2 to supply power to water pump 2. A switch and external power cord are installed near the motor. Holes are drilled near the water pump, forming one water inlet 8 and five water outlets 9. The number of outlets is not limited to five; it can be set to six, seven, etc., as needed. The breathing rings are not limited to the first breathing ring 3-1, second breathing ring 3-2, third breathing ring 3-3, fourth breathing ring 3-4, fifth breathing ring 3-5, sixth breathing ring 3-6, and seventh breathing ring 3-7; additional breathing rings can be added as needed.

[0054] The breathing ring consists of two cylindrical PVC pipes, including an inner pipe 3-1-1 and an outer pipe 3-1-2. The diameter of the outer pipe 3-1-2 is 2cm larger than that of the inner pipe. The inner pipe 3-1-1 is 3-5cm longer than the outer pipe 3-1-2. This part is inserted into the soil when collecting gas. The outer pipe 3-1-2 is fitted over the inner pipe, with a 1cm gap between the two pipes. The tops of the two pipes are flush. The bottom of the inner pipe 3-1-1 is 3-5cm longer than that of the outer pipe 3-1-2. The outer pipe 3-1-2 and the inner pipe 3-1-1 are sealed with a circular ring, and the joint is sealed with sealant. The space between the two pipes is the water circulation space. The outer pipe 3-1-2 has two holes of different heights. The higher one is the breathing ring inlet 3-1-3, and the lower one is the breathing ring outlet 3-1-4. The top of the breathing ring is equipped with a rubber cap 3-1-6, which is used to seal the soil when collecting breathing gas.

[0055] Water pipe 4 is a high-temperature resistant silicone hose that connects to each water inlet 8, water pump 2, water outlet 9, and breather ring 3, allowing water to circulate throughout the device.

[0056] The bucket 5 is filled with water, and one end of the water inlet 8 and water pipe 4 is placed in the bucket 5 to supply water to the circulation device. In addition, one end of the breathing ring outlet 3-1-4 is also placed in the bucket to collect the circulating water. At the same time, ice cubes are placed in the bucket to keep the water temperature consistent with the ambient temperature.

[0057] A method for collecting respiration gases from soil using a cooling device includes the following steps:

[0058] Step S1: According to the sampling requirements, set up the breathing ring and insert the breathing ring into the soil of the sampling area. The insertion depth is the length of the inner tube that is longer than the outer tube.

[0059] Step S2: Place a small beaker containing NaOH solution in each breathing ring, cover it with a rubber cap to seal it, and connect the water pipe to the inlet and outlet of each breathing ring to make the entire water circulation connected.

[0060] Step S3: Fill the bucket with water, put the other end of the water pipe inserted into the water pump inlet into the bucket, and put the last end of the breathing ring outlet into the bucket. This satisfies the water intake and ensures that the water at the outlet is not wasted, so that the water supply can continue to be circulated.

[0061] Step S4: Use a thermometer to measure the temperature of the water in the bucket and determine whether the water temperature is the same as the ambient air temperature. If they are the same, proceed to step S5; if they are not the same, add ice cubes to cool the water in the bucket.

[0062] Step S5: After all preparations are complete, plug in the power, turn on the switch, and the water pump will start pumping water to circulate the entire water system and ensure that the temperature inside the breathing ring does not rise as the placement time increases.

[0063] Step S6: After collecting the gas according to the sampling time designed in the experiment, turn off the power, open the rubber cap on the breathing ring, and take out the beaker for subsequent measurement of the amount of CO2 in soil respiration.

[0064] In this implementation plan, breathing rings are deployed according to sampling requirements. The breathing rings are inserted into the soil of the sampling area to a depth equal to the length of the inner tube exceeding the outer tube. A small beaker containing NaOH solution is placed inside each breathing ring, and the rings are sealed with rubber caps. Water pipes are connected to the various inlets and outlets, connecting the entire water circulation system. A bucket is filled with water, and the other end of the water pipe inserted into the water pump inlet is placed into the bucket. The final outlet end of the breathing ring is also placed into the bucket. This satisfies the water intake requirements for water circulation while ensuring no water is wasted at the outlet, thus supplying water for circulation. Continue using the device to achieve water conservation; measure the water temperature in the bucket with a thermometer, and determine whether to add ice to cool the water based on whether the water temperature matches the ambient temperature; after all preparations are complete, plug in the power, turn on the switch, and the water pump will start pumping water to circulate the entire water system, ensuring that the temperature inside the breathing ring does not rise with the increase in storage time; after collecting the gas according to the sampling time designed in the experiment, turn off the power, open the lid on the breathing ring, and take out the small beaker for subsequent measurement of the amount of CO2 in soil respiration.

Claims

1. A cooling device for collecting respiration gases from soil, characterized in that, Includes motor (1), water pump (2), breathing ring (3), water pipe (4), water bucket (5), switch (6), external power cord (7), water inlet (8), water outlet (9) and ice (10); The motor (1) and the water pump (2) are assembled together in a sheet metal assembly box. The motor (1) is fixed on the cover on the top surface of the sheet metal assembly box, and the water pump (2) is fixed on the base of the sheet metal assembly box. The motor (1) and the water pump (2) are connected by wires. The switch (6) and the external power cord (7) are installed at the back of the sheet metal assembly box. An inlet (8) is set on the side of the sheet metal assembly box and near the water pump. An outlet (9) is set at the front of the sheet metal assembly box. The outlet (9) is connected to the breathing ring (3) through the water pipe (4). The breathing ring (3) is connected to the water bucket (5) through the water pipe (4). The water bucket (5) is connected to the inlet (8) of the sheet metal assembly box through the water pipe (4). The ice block (10) is placed in the water bucket (5). The water outlets include a first water outlet (9-1), a second water outlet (9-2), a third water outlet (9-3), a fourth water outlet (9-4), and a fifth water outlet (9-5). The breathing ring (3) includes a first breathing ring (3-1), a second breathing ring (3-2), a third breathing ring (3-3), a fourth breathing ring (3-4), a fifth breathing ring (3-5), a sixth breathing ring (3-6), and a seventh breathing ring (3-7). The fifth outlet (9-5) is connected to the first breathing ring (3-1), the first breathing ring (3-1) is connected to the second breathing ring (3-2), the second breathing ring (3-2) is connected to the third breathing ring (3-3), and the third breathing ring (3-3) is connected to the water bucket (5). The fourth outlet (9-4) is connected to the fourth breathing ring (3-4), the fourth breathing ring (3-4) is connected to the fifth breathing ring (3-5), and the fifth breathing ring (3-5) is connected to the water bucket (5). The third outlet (9-3) is connected to the sixth breathing ring (3-6), the sixth breathing ring (3-6) is connected to the seventh breathing ring (3-7), and the seventh breathing ring (3-7) is connected to the water bucket (5). The second outlet (9-2) and the first outlet (9-1) are plugged when not in use; The first breathing ring (3-1) includes an inner tube (3-1-1), an outer tube (3-1-2), a breathing ring inlet (3-1-3), a breathing ring outlet (3-1-4), a beaker (3-1-5), and a rubber cap (3-1-6). The beaker (3-1-5) is disposed inside the inner tube (3-1-1), which is disposed inside the outer tube (3-1-2). The inner tube (3-1-1) and the outer tube (3-1-2) are spaced 1 cm apart. The top ends of the inner tube (3-1-1) and the outer tube (3-1-2) are flush, and the bottom end of the inner tube (3-1-1) extends 3-5 cm beyond the outer tube (3-1-2). cm, the outer tube (3-1-2) and the inner tube (3-1-1) are sealed with a circular ring, and the joint is sealed with sealant. The space between the outer tube (3-1-2) and the inner tube (3-1-1) is a water circulation space. The breathing ring inlet (3-1-3) is located on one side of the outer tube (3-1-2) and close to the top surface of the outer tube (3-1-2). The breathing ring outlet (3-1-4) is located on the other side of the outer tube (3-1-2) and is lower than the height of the breathing ring inlet (3-1-3). The rubber cap (3-1-6) is located at the top of the first breathing ring (3-1) and is used to cover and seal when collecting soil breathing gas. NaOH solution is placed in the beaker. The first breathing ring (3-1), the second breathing ring (3-2), the third breathing ring (3-3), the fourth breathing ring (3-4), the fifth breathing ring (3-5), the sixth breathing ring (3-6), and the seventh breathing ring (3-7) have the same structure.

2. The cooling device for collecting soil respiration gases according to claim 1, characterized in that, The motor (1) and the water pump (2) are connected by wires for supplying power to the water pump (2).

3. The cooling device for collecting soil respiration gases according to claim 1, characterized in that, Both the inner tube (3-1-1) and the outer tube (3-1-2) are PVC pipes; the diameter of the outer tube (3-1-2) is 2 cm larger than that of the inner tube.

4. A cooling device for collecting soil respiration gases according to claim 1, characterized in that, The water pipe (4) is a silicone hose that connects the water bucket (5), the water inlet (8), the water pump (2), the water outlet (9), and the breathing ring (3) to make the water flow through the entire device.

5. The cooling device for collecting soil respiration gases according to claim 1, characterized in that, The water bucket (5) is used to hold water. One end of the water outlet (3-1-4) of the breathing ring is connected to the water pipe (4). The water pipe (4) of the water outlet is placed in the water bucket (5) to collect the circulating water. The water inlet (8) is connected to the water pipe (4). The other end of the water pipe (4) is placed in the water bucket to supply water to the circulation device.

6. A method for collecting respiration gases from soil using a cooling device, characterized in that, The apparatus of claim 1 comprises the following steps: Step S1: According to the sampling requirements, set up the breathing ring and insert the breathing ring into the soil of the sampling area. The insertion depth is the length of the inner tube that is longer than the outer tube. Step S2: Place a small beaker containing NaOH solution in each breathing ring, cover it with a rubber cap to seal it, and connect the water pipe to the inlet and outlet of each breathing ring to make the entire water circulation connected. Step S3: Fill the bucket with water, put the other end of the water pipe inserted into the water pump inlet into the bucket, and put the last end of the breathing ring outlet into the bucket. This satisfies the water intake and ensures that the water at the outlet is not wasted, so that the water supply can continue to be circulated. Step S4: Use a thermometer to measure the temperature of the water in the bucket and determine whether the water temperature is the same as the ambient air temperature. If they are the same, proceed to step S5; if they are not the same, add ice cubes to cool the water in the bucket. Step S5: After all preparations are complete, plug in the power, turn on the switch, and the water pump will start pumping water to circulate the entire water system and ensure that the temperature inside the breathing ring does not rise as the placement time increases. Step S6: After collecting the gas according to the sampling time designed in the experiment, turn off the power, open the rubber cap on the breathing ring, and take out the beaker for subsequent measurement of the amount of CO2 in soil respiration.

Images