A detachable electrode support and electrophoresis device
By designing the disassembled electrode bracket, the high replacement cost and complex operation problems of electrode brackets in the electrophoresis device are solved, and the rapid replacement and simplified operation of the brackets and wires are achieved.
Patent Information
- Application Number
- CN202410124425.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-29
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2044-01-29
AI Technical Summary
In existing electrophoretic devices, damage to the electrode bracket requires replacement of the entire bracket or rewinding the wire, which is costly and complicated to operate.
A disassembleable electrode bracket is designed, including a support frame body, a disassembled positive electrode assembly and a negative electrode assembly. The exposed section lengths of the positive electrode wire and the negative electrode wire are adapted to form a uniform electric field, and each part is replaced independently without wrapping.
The replacement process of the electrode bracket is simplified, the replacement cost and operation difficulty are reduced, and the rapid replacement of the bracket and wire is achieved.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of electrophoresis tanks for biological protein gels, and in particular relates to a detachable electrode support and an electrophoresis device. Background Art
[0002] In molecular biology experiments, the use of gel electrophoresis to detect experimental results is an indispensable experimental method, and in the experiment, the electrophoresis device is an indispensable experimental tool.
[0003] The existing electrophoresis device mainly includes a tank cover, an electrophoresis tank and an electrode holder. The electrode holder is mainly composed of a support frame and positive and negative electrode assemblies. In the prior art, the support frame and the positive and negative electrode assemblies are all integrated, a wiring groove is opened on the support frame, and then the positive and negative electrode wires are wound. When the positive and negative electrode wires or the support frame are damaged, it is often necessary to replace the entire electrode holder or rewind the positive and negative electrode wires. The positive and negative electrode wires are generally platinum wires that are easy to break and difficult to wind. If the entire holder is replaced, the cost will be relatively high. Summary of the invention
[0004] On one hand, the present invention provides a detachable electrode bracket to solve the above-mentioned problems that the electrode bracket is damaged, the positive and negative electrode wires are not easy to be entangled or the cost of replacing the entire electrode bracket is high. On the other hand, the present invention also provides an electrophoresis device.
[0005] In order to achieve the above object, the present invention adopts the following technical solution:
[0006] A detachable electrode bracket, comprising:
[0007] The support frame body has an open first cavity, and a mounting position is provided on the support frame body;
[0008] A positive electrode assembly, which can be detachably arranged on the mounting position of the support frame body, the positive electrode assembly is located outside the first cavity of the support frame body, the positive electrode assembly includes a positive electrode bracket, a positive electrode terminal arranged on the positive electrode bracket, and a positive electrode wire arranged on the positive electrode bracket, the positive electrode wire includes a first insulating section and a first exposed section, and one end of the first insulating section of the positive electrode wire is connected to the positive electrode terminal;
[0009] A negative electrode assembly can be removably arranged on the mounting position of the support frame body, wherein the negative electrode assembly is partially located in the first cavity of the support frame body, and the negative electrode assembly is independently arranged relative to the positive electrode assembly, wherein the negative electrode assembly comprises a negative electrode support, a negative electrode terminal arranged on the negative electrode support, and a negative electrode wire arranged on the negative electrode support, wherein one end of the negative electrode wire is connected to the negative electrode terminal, and the negative electrode wire comprises a second insulating section and a second exposed section, wherein the second exposed section of the negative electrode wire is adapted in length to the first exposed section of the positive electrode wire and corresponds in position, and when powered on, the first exposed section of the positive electrode wire and the second exposed section of the negative electrode wire can form a uniform electric field.
[0010] In some embodiments, the mounting position includes a first mounting groove adapted to the positive electrode bracket and a second mounting groove adapted to the negative electrode bracket, the positive electrode bracket is arranged on the first mounting groove, and the negative electrode bracket is arranged on the second mounting groove.
[0011] In some embodiments, the thickness of the positive electrode support is less than or equal to the depth of the first mounting groove, and the thickness of the negative electrode support is less than or equal to the depth of the second mounting groove.
[0012] In some embodiments, the second mounting groove is arranged on one side of the first cavity and is connected to the first cavity. When the negative electrode bracket is installed on the second mounting groove, the negative electrode bracket is partially placed in the first cavity, and the negative electrode bracket abuts against the inner wall of the first cavity.
[0013] In some embodiments, a first wiring groove is provided on the positive electrode bracket, and the first insulating section of the positive electrode wire is arranged in the first wiring groove.
[0014] In some embodiments, a first fixing structure is provided on a side of the first wiring groove away from the positive electrode terminal, and a first exposed section of the positive electrode wire is fixedly disposed on the positive electrode bracket through the first fixing structure.
[0015] In some embodiments, the positive electrode wire and the positive electrode bracket are integrally arranged.
[0016] In some embodiments, a second wiring groove is provided on the negative electrode support, and the second insulating section of the negative electrode wire is arranged in the second wiring groove.
[0017] In some embodiments, a second fixing structure is provided on a side of the second wiring groove away from the negative electrode terminal, and the second exposed section of the negative electrode wire is fixedly disposed on the negative electrode bracket through the second fixing structure.
[0018] In some embodiments, the negative electrode lead and the negative electrode support are integrally arranged.
[0019] In some embodiments, the first exposed section of the positive electrode wire is disposed horizontally, and the second exposed section of the negative electrode wire is disposed parallel to the first exposed section of the positive electrode wire.
[0020] In some embodiments, the distance between the second exposed section of the negative electrode wire and the bottom wall of the support frame body is half the depth of the first cavity of the support frame body.
[0021] In some embodiments, a sealing groove is provided on the support frame body, and a sealing strip is provided on the sealing groove.
[0022] In some embodiments, the present invention also provides an electrophoresis device, including an electrophoresis tank, having a second cavity and a second notch connecting the second cavity to the outside; as described in the above embodiments, the detachable electrode bracket is arranged in the second cavity.
[0023] Compared with the prior art, the present invention has the following beneficial effects:
[0024] The present application arranges the traditional integrated electrode support into a detachable support frame body, a positive electrode assembly and a negative electrode assembly, wherein the positive electrode support of the positive electrode assembly can be detachably arranged on the support frame body, and the negative electrode support of the negative electrode assembly can be detachably arranged on the support frame body, thereby enabling replacement of any one of the support frame body, the positive electrode assembly and the negative electrode assembly, without the need to wind the positive and negative electrode wires or replace the entire electrode support, thereby reducing the difficulty of replacement, simplifying the operation and reducing the replacement cost. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of assembling a detachable electrode bracket of the present invention;
[0026] Figure 2 It is a schematic diagram of a first three-dimensional structure of a support frame body of a detachable electrode support of the present invention;
[0027] Figure 3 It is a schematic diagram of a second three-dimensional structure of a support frame body of a detachable electrode support of the present invention;
[0028] Figure 4 It is a schematic diagram of the three-dimensional structure of a positive electrode support of a detachable electrode support of the present invention;
[0029] Figure 5 A top view of a positive electrode support of a detachable electrode support of the present invention;
[0030] Figure 6It is a schematic diagram of the three-dimensional structure of a negative electrode support of a detachable electrode support of the present invention;
[0031] Figure 7 A top view of a negative electrode support of a detachable electrode support of the present invention;
[0032] Figure 8 It is a schematic diagram of the three-dimensional structure of the electrophoresis tank of the electrophoresis device of the present invention;
[0033] Fig. 9 This is a schematic diagram of the structure of the assembled electrode bracket of the present invention installed in the electrophoresis tank; DETAILED DESCRIPTION
[0034] The present application is further described in detail below in conjunction with specific drawings. In the description of this embodiment, unless otherwise specified, the terms "left", "right", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, which are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the present application must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present application.
[0035] like Figure 1-3 As shown in the figure, a detachable electrode support provided by the present invention mainly includes a support frame body 100, a positive electrode assembly 200 and a negative electrode assembly 300. The support frame body 100 has an open first cavity 101, and a mounting position is provided on the support frame body 100 for detachable installation of the positive electrode assembly 200 and the negative electrode assembly 300. In this embodiment, as shown in FIG. Figure 2As shown, the electrode support is a structure for placing a single rubber plate, one side wall of the electrode support is a sealing structure, and the other side is open, and a corresponding sealing groove 102 is provided. The positive electrode assembly 200 is detachably arranged on the support frame body 100, and the positive electrode assembly 200 is located outside the first cavity 101 of the support frame body 100. The positive electrode assembly 200 includes a positive electrode support 202, a positive electrode terminal 201 arranged on the positive electrode support 202, and a positive electrode wire arranged on the positive electrode support 202. The positive electrode wire is composed of a first insulating section and a first exposed section. One end of the first insulating section of the positive electrode wire is electrically connected to the positive electrode terminal 201, and the first exposed section of the positive electrode wire is fixed to the outside of the positive electrode support 202; the negative electrode assembly 300 is detachably arranged on the support frame body 100, and the negative electrode support 302 is located in the first cavity 101 of the support frame body 100 In the embodiment, the negative electrode assembly 300 is relatively independently arranged relative to the positive electrode assembly 200, that is, the negative electrode assembly 300 is not electrically connected with the positive electrode assembly 200, and the negative electrode assembly 300 includes a negative electrode bracket 302, a negative electrode terminal 301 arranged on the negative electrode bracket 302, and a negative electrode wire arranged on the negative electrode bracket 302, the negative electrode wire is composed of a second insulating section and a second exposed section, one end of the second insulating section of the negative electrode wire is electrically connected to the negative electrode terminal 301, the second exposed section of the negative electrode wire matches the first exposed section of the positive electrode wire in length and has a corresponding installation position, and in the energized state, the first exposed section of the positive electrode wire and the second exposed section of the negative electrode wire can form a uniform electric field to meet experimental needs. In this embodiment, the positive electrode wire and the negative electrode wire are both platinum wires. In order to save experimental costs, the first insulating section of the positive electrode wire and the second insulating section of the negative electrode wire can be other metal conductors that can be energized, such as copper or silver, and the first exposed section of the positive electrode wire and the second exposed section of the negative electrode wire are both platinum wires.
[0036] In the present application, by disassembling the traditional one-piece electrode bracket into a support bracket body 100, a positive electrode assembly 200 and a negative electrode assembly 300 that can be detachably connected, when a component of the electrode bracket is damaged, there is no need to wind and assemble the platinum wire or replace the entire electrode bracket, thereby simplifying the component replacement process. Experimenters do not need to return the product to the factory for repair, but can disassemble and replace the corresponding components, thereby reducing experimental costs.
[0037] In one embodiment, Figure 2As shown, the mounting position includes a first mounting groove 104 and a second mounting groove 105 provided on the support frame body 100, the first mounting groove 104 is adapted to the positive electrode bracket 202, and the second mounting groove 105 is adapted to the negative electrode bracket 302. The positive electrode bracket 202 is mounted on the first mounting groove 102, and the negative electrode bracket 302 is mounted on the second mounting groove 105. Specifically, in the present embodiment, the positive electrode bracket 202 is a Z-shaped structure, which can be bridged from the upper end of the support frame body 100 to the lower end of the support frame body 100, so that the positive electrode wire can reach the outer position of the lower end of the support frame body 100, and the first mounting groove 104 is also a Z-shaped structure, and its size is adapted to the size of the positive electrode bracket 202. The positive electrode bracket 202 is snapped onto the first mounting groove 104, and a plurality of first through holes 2022 are provided on the positive electrode bracket 202, and a plurality of corresponding first threaded holes 106 are provided on the support frame body 100, which are screwed in place. The positive electrode bracket 202 is fixed to the support frame body 100; the second mounting groove 105 is a straight-line structure, spanning the two side walls of the support frame body 100, so that the second exposed section of the negative electrode wire can extend from one side wall of the first cavity 101 to the other opposite side wall, so that the prefabricated glue on the glue board is in the electric field coating, a plurality of second through holes 3022 are provided on the negative electrode bracket 302, and corresponding second threaded holes 107 are provided on the support frame body 100, and the negative electrode bracket 302 is locked and fixed to the support frame body 100 by screws. In this embodiment, in order to facilitate the direct installation of the negative electrode wire in the first cavity 101, the second mounting groove 105 is extended to the inner wall of the first cavity 101 and connected to the first cavity 101.
[0038] Furthermore, if Figure 1 and Figure 5 As shown, in order to facilitate the assembly of the entire electrode bracket, the thickness A of the positive electrode bracket 202 is less than or equal to the depth of the first mounting groove 104. That is, when the positive electrode bracket 202 is installed on the first mounting groove 104, the positive electrode bracket 202 will not protrude relative to the side of the support frame body 100. In this embodiment, as a preferred assembly method, the thickness of the positive electrode bracket 202 is consistent with the depth of the first mounting groove 104, and the positive electrode bracket 202 can be limited, supported and fixed when the electrode bracket is installed, and no complicated notches are required to install the electrode bracket. Similarly, Figure 1 and Figure 7 As shown, the thickness B of the negative electrode support 302 is also consistent with the depth of the second mounting groove 105, and the principle is the same as that of the positive electrode support 202, which will not be described in detail here.
[0039] Furthermore, since part of the negative electrode bracket 302 needs to be installed in the first cavity 101, in order to make the structure of the negative electrode bracket 302 installation more stable, when part of the negative electrode bracket 302 is placed in the first cavity 101, the negative electrode bracket 302 located in the first cavity 101 abuts against the inner wall of the first cavity 101 of the support frame body 100. By abutting the negative electrode bracket 302 against the inner wall of the first cavity 101, positioning can be directly performed to prevent the negative electrode bracket 302 from moving relative to the support frame body 100.
[0040] In one embodiment, in order to facilitate the installation of the positive electrode wire, the positive electrode wire is wound around the positive electrode bracket 202, as shown in FIG. Figure 4 As shown, a first wiring groove 2021 is provided on the positive electrode support 202, and the first wiring groove 2021 is distributed along the axial direction of the positive electrode support 202, and the first wiring groove 2021 is provided along the side of the positive electrode support 202 close to the support frame body 100, and the first insulating section of the positive electrode wire is located in the first wiring groove 2021. When the positive electrode support 202 is installed on the support frame body 100, the support frame body 100 covers the first wiring groove 2021, and further insulates the positive electrode wire. Furthermore, in order to fix the first exposed section of the positive electrode wire, a first fixing structure 2023 is provided on the side of the first wiring groove 2021 away from the positive electrode terminal 201, and the first exposed section of the positive electrode wire is fixed to the outside of the support frame body 100 through the first fixing structure 2023. Specifically, in this embodiment, the first fixing structure 2023 is two first fixing holes, and the two first fixing holes are set at a preset distance. When the first exposed section of the positive electrode wire is fixed on it, the prefabricated glue of the rubber plate can be wrapped inside to meet the test needs. Optionally, the first fixing hole can also be set in the form of a fixing column. It can be known that the shape and structure of the first fixing structure 2023 are not limited by the present invention. In order to further simplify the assembly process, the positive electrode wire and the positive bracket 202 are set as an integrated structure, that is, the first insulating section of the positive electrode wire is embedded in the positive bracket 202, and at the same time, the protection of the first insulating section of the positive electrode wire can be improved.
[0041] In one embodiment, similarly, in order to facilitate the installation of the negative electrode wire, it is wound around the negative electrode bracket 302, such as Figure 6As shown, a second wiring groove 3021 is provided on the negative electrode support 302, and the second insulating section of the negative electrode wire is provided in the second wiring groove 3021. Specifically, the second wiring groove 3021 is provided on the side of the negative electrode support 302 close to the support frame body 100. When the negative electrode support 302 is installed on the support frame body 100, the support frame body 100 covers the second wiring groove 3021, and further insulates the negative electrode wire. Furthermore, a second fixing structure 3023 is provided on the side of the second wiring groove 3021 away from the negative electrode terminal 301, and the second exposed section of the negative electrode wire is fixedly provided on the negative electrode support 302 through the second fixing structure 3023. By providing the second fixing structure 3023, the second exposed section of the negative electrode wire is fixed, so that the second exposed section of the negative electrode wire and the first exposed section of the positive electrode wire are correspondingly provided. After power is turned on, the first exposed section of the positive electrode wire and the second exposed section of the negative electrode wire can form a uniform and stable electric field. In this embodiment, the second fixing structure 3023 is two second fixing holes arranged at intervals. Optionally, the second fixing structure 3023 can also be in the form of fixing columns arranged at intervals. Further, the negative electrode wire and the negative electrode bracket 302 can also be arranged as an integrated structure, that is, the second insulating section of the negative electrode wire is embedded in the negative electrode bracket 302, which simplifies the replacement of accessories and further improves the protection of the second insulating section of the negative electrode wire, thereby increasing its service life.
[0042] In one embodiment, again referring to Figure 1 In order to ensure a relatively stable and balanced electric field, the first exposed section of the positive wire is arranged horizontally, and the second exposed section of the negative wire is arranged parallel to the first exposed section of the positive wire. Furthermore, in order to achieve a better effect of the running gel test, since the running gel test is sensitive to temperature, it is necessary to avoid the influence of temperature concentration on the running gel result. It is found through experiments that the distance between the second exposed section of the negative wire and the bottom wall of the support frame body 100 is half the depth of the first cavity 101 of the support frame body 100. In this embodiment, the positive wire is arranged close to the outer bottom wall of the support frame body 100.
[0043] In one embodiment, a sealing groove is provided on the support frame body 100, and a sealing rubber strip is provided on the sealing groove 102. Specifically, in this embodiment, it is a glue running experiment of prefabricated glue on a single glue plate, so the sealing groove 102 is only provided on one side of the support frame body 100, and an integral side wall is provided on the other side of the support frame body 100 for sealing. Optionally, when two glue plates are provided on both sides, such as Figure 2As shown, the side wall of the support frame body 100 close to the second installation groove 105 is removed, and the sealing groove 102 and the sealing strip are set at the same time, so that the glue running experiment of the two rubber sheets can be realized. Furthermore, the sealing strip can be detachably set on the sealing groove 102, wherein different step structures are pre-set on the side of the opening of the sealing strip close to the support frame body 100 to match the hand-filled glue and prefabricated glue of different sizes commonly used in the market. The hand-filled glue and prefabricated glue are both available in two sizes of 80mm*100* and 100mm*100mm. When the rubber sheet is equipped with hand-filled glue or prefabricated glue of different sizes, different boss structures will be formed on the rubber sheet. In order to ensure the sealing performance of the rubber sheet equipped with two different sizes of hand-filled glue or prefabricated glue, by pre-setting the corresponding step structure on the sealing strip, the sealing strips with step structures of different heights are selectively installed according to the experimental requirements.
[0044] In one embodiment, Figure 8-9 As shown, the present invention further provides an electrophoresis device, including an electrophoresis tank 400, provided with a second cavity 401 and a second notch connecting the second cavity 401 with the outside, wherein a first positioning groove 402 is provided on the electrophoresis tank 400; as described in the above-mentioned various embodiments, the electrode holder is arranged in the second cavity 401, wherein a first positioning boss 108 is provided on the support frame body 100, and the first positioning boss 108 is adapted to the first positioning groove 402, and when the electrode holder is arranged on the electrophoresis tank 400, the upper end surface of the electrode holder is not higher than the upper end surface of the electrophoresis tank 400. When the electrode holder is installed in the second cavity 401, a preset spacing 403 is provided between the electrode holder and the inner wall of the electrophoresis tank 400 for the installation of the rubber plate.
[0045] The above is only a preferred embodiment of the present invention. It should be noted that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications should also be considered as the protection scope of the present invention.
Claims
1. A detachable electrode holder, characterized in that: include: The support frame body has an open first cavity, and a mounting position is provided on the support frame body; A positive electrode assembly, which can be detachably arranged on the mounting position of the support frame body, the positive electrode assembly is located outside the first cavity of the support frame body, the positive electrode assembly includes a positive electrode bracket, a positive electrode terminal arranged on the positive electrode bracket, and a positive electrode wire arranged on the positive electrode bracket, the positive electrode wire includes a first insulating section and a first exposed section, and one end of the first insulating section of the positive electrode wire is connected to the positive electrode terminal; A negative electrode assembly, which can be detachably arranged on the mounting position of the support frame body, the negative electrode assembly is independently arranged relative to the positive electrode assembly, the negative electrode assembly comprises a negative electrode bracket, a negative electrode terminal arranged on the negative electrode bracket, and a negative electrode wire arranged on the negative electrode bracket, one end of the negative electrode wire is connected to the negative electrode terminal, the negative electrode wire comprises a second insulating section and a second exposed section, wherein the second exposed section of the negative electrode wire is adapted to the length of the first exposed section of the positive electrode wire, the first exposed section of the positive electrode wire is arranged horizontally, and the second exposed section of the negative electrode wire is arranged parallel to the first exposed section of the positive electrode wire, and in the power-on state, the first exposed section of the positive electrode wire and the second exposed section of the negative electrode wire can form a uniform electric field; Wherein, the mounting position includes a first mounting groove adapted to the positive electrode bracket and a second mounting groove adapted to the negative electrode bracket, the positive electrode bracket is arranged on the first mounting groove, the negative electrode bracket is arranged on the second mounting groove, the thickness of the positive electrode bracket is less than or equal to the depth of the first mounting groove, and the thickness of the negative electrode bracket is less than or equal to the depth of the second mounting groove; The second mounting groove is arranged near one side of the first cavity and communicates with the first cavity. When the negative electrode support is mounted on the second mounting groove, the negative electrode support is partially placed in the first cavity, and the negative electrode support abuts against the inner wall of the first cavity.
2. The detachable electrode holder according to claim 1, characterized in that: The positive electrode bracket is provided with a first wiring groove, and the first insulating section of the positive electrode wire is arranged in the first wiring groove.
3. The detachable electrode holder according to claim 2, characterized in that: A first fixing structure is provided on a side of the first wiring groove away from the positive electrode terminal, and a first exposed section of the positive electrode wire is fixedly arranged on the positive electrode bracket through the first fixing structure.
4. The detachable electrode holder according to claim 1, characterized in that: The positive electrode wire and the positive electrode bracket are arranged in one piece.
5. The detachable electrode holder according to claim 1, characterized in that: The negative electrode support is provided with a second wiring groove, and the second insulating section of the negative electrode wire is arranged in the second wiring groove.
6. The detachable electrode holder according to claim 5, characterized in that: A second fixing structure is provided on a side of the second wiring groove away from the negative electrode terminal, and the second exposed section of the negative electrode wire is fixedly arranged on the negative electrode bracket through the second fixing structure.
7. The detachable electrode holder according to claim 1, characterized in that: The negative electrode wire and the negative electrode bracket are arranged in one piece.
8. The detachable electrode holder according to claim 1, characterized in that: The distance between the second exposed section of the negative electrode wire and the bottom wall of the support frame body is half the depth of the first cavity of the support frame body.
9. The detachable electrode holder according to claim 1, characterized in that: The support frame body is provided with a sealing groove, and the sealing groove is provided with a sealing rubber strip.
10. An electrophoresis device, characterized in that include: An electrophoresis tank, provided with a second cavity and a second notch connecting the second cavity with the outside; The detachable electrode holder according to any one of claims 1 to 9, wherein the electrode holder is disposed in the second cavity.