A PCL-BPNSs-PDA@Mn3O4 nanofiber membrane and a preparation method thereof

Abstract

The application provides a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane and a preparation method thereof, PDA is wrapped on the surfaces of BPNSs and Mn3O4, the cytotoxicity of Mn3O4 can be effectively reduced, the prepared BPNSs-PDA@Mn3O4 has the characteristics of the three materials, and meanwhile, the BPNSs-PDA@Mn3O4 is introduced into PCL, and the electrostatic spinning method is used to prepare the PCL-BPNSs-PDA@Mn3O4 nanofiber membrane with the ability of promoting wound healing.

Description

Technical Field

[0001] This invention relates to nanofiber membranes, and more particularly to a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane and its preparation method. Background Technology

[0002] Wound healing is a highly complex process involving a series of dynamic events, including hemostasis, inflammation, cell proliferation, angiogenesis and granulation tissue formation, and skin epithelialization. It is also susceptible to various factors, with infection being one of the most common causes of delayed healing in different types of wounds. Nanomaterials can effectively promote wound healing, photothermal-induced cell function regulation aids in tissue repair and regeneration, and photothermal antibacterial therapy can generate reactive oxygen species that react with the surrounding environment to damage target microorganisms.

[0003] For chronically infected skin wounds, the drug resistance of pathogens and the difficulty of intravenous or oral medications effectively reaching the wound site result in poor treatment outcomes for these wounds. Therefore, wound dressings have become an effective adjunct to promote skin tissue repair and healing. While traditional dressings such as gauze can keep the wound area dry and absorb exudate, they cannot create a moist environment for tissue repair and reconstruction. Researchers have been studying various advanced antibacterial materials, including some heavy metal ions and natural extracts; however, the cytotoxicity of these substances limits their practical application. Nanofiber membranes prepared using electrospinning technology can make more effective contact with the wound, providing a favorable healing environment.

[0004] Electrospun raw materials possess excellent biocompatibility and biodegradability, making them suitable for use as extracellular scaffolds and carriers, promoting cell and tissue growth. Examples include polycaprolactone (PCL), polyvinyl alcohol (PVA), polylactic acid-co-glycolic acid (PLGA), and polylactic acid (PLLA). Among these, PCL exhibits superior biocompatibility, organic polymer compatibility, and biodegradability. Furthermore, electrospun nanofibers possess excellent properties such as large specific surface area and porosity. Their morphology and structure, along with nanofiber biomembranes similar to the extracellular matrix, provide a matrix for cell adhesion, proliferation, and growth. Current research primarily focuses on improving the formulation of electrospun nanomembranes to achieve better biocompatibility and mechanical properties.

[0005] Mn3O4 nanoparticles possess the function of antioxidant enzymes, and their preparation method is simple. The synthesized nanoparticles exhibit good biostability, biodegradability, and low cost. However, previous studies have found that Mn3O4 has poor biocompatibility, strong cytotoxicity, and a small particle size that makes it prone to aggregation, severely limiting its application as a nanozyme in disease treatment. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a method for preparing PCL-BPNSs-PDA@Mn3O4 nanofiber membrane. By coating PDA on the surface of BPNSs and Mn3O4, the cytotoxicity of Mn3O4 can be effectively reduced, so that the prepared BPNSs-PDA@Mn3O4 has the characteristics of the three materials. At the same time, BPNSs-PDA@Mn3O4 is introduced into PCL, and the PCL-BPNSs-PDA@Mn3O4 nanofiber membrane with wound healing ability is prepared by electrospinning.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0008] A method for preparing a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane includes the following steps:

[0009] S1. Disperse black phosphorus powder in N-methylpyrrolidone, place it in an ice bath under light-protected conditions, and sonicate it for 8-12 hours using an ultrasonic cell disruptor. Then, centrifuge for 8-12 minutes to obtain the supernatant. Centrifuge the supernatant for 10 minutes to obtain the precipitate. Wash the precipitate three times with anhydrous ethanol to remove N-methylpyrrolidone and obtain a black phosphorus dispersion. Vacuum dry the black phosphorus dispersion to constant weight to obtain black phosphorus nanosheets (BPNSs).

[0010] S2. Add the BPNSs obtained in step S1 to ultrapure water and sonicate for 3-5 minutes to obtain a uniformly dispersed BPNSs suspension. Add ammonia and anhydrous ethanol to the BPNSs suspension and stir magnetically for 25-35 minutes to obtain mixture one.

[0011] S3. Dissolve dopamine hydrochloride in ultrapure water to obtain a dopamine solution, add the dopamine solution dropwise to the first mixture obtained in step S2, and stir for 20-24 hours to obtain the second mixture;

[0012] S4. Dissolve manganese nitrate in ultrapure water to obtain manganese nitrate solution, add manganese nitrate solution dropwise to mixture two obtained in step S3, and stir magnetically for 6-24 hours to obtain mixture three;

[0013] S5. Dissolve ascorbic acid in ultrapure water to obtain ascorbic acid solution. Add the ascorbic acid solution dropwise to the mixture obtained in step S4. Stir for 6-24 hours and centrifuge for 10 minutes. Discard the supernatant and resuspend the precipitate. Centrifuge 2-4 times in the same way to obtain the precipitate. Dry the precipitate under vacuum to constant weight to obtain BPNSs-PDA@Mn3O4.

[0014] S6. Add the BPNSs-PDA@Mn3O4 obtained in step S5 to hexafluoroisopropanol, stir for 10-15 h, sonicate for 25-35 min, add PCL aqueous solution, stir for 10-15 h, sonicate for 25-35 min to obtain electrospinning solution, place the electrospinning solution on the syringe of the electrospinning machine to perform electrospinning to obtain fiber membrane, place the fiber membrane in a vacuum drying oven to dry to constant weight to obtain PCL-BPNSs-PDA@Mn3O4 nanofiber membrane.

[0015] Furthermore, in step S1 of the present invention, the ratio of black phosphorus powder to N-methylpyrrolidone is 10 mg: 1 mL; the ultrasonic power of the ultrasonic cell disruptor is 200 W; the centrifugation speed during the first centrifugation is 3000 r / min; and the centrifugation speed during the second centrifugation is 12000 r / min.

[0016] Furthermore, in step S2 of the present invention, the mass concentration of ammonia is 20%, and the ratio of BPNSs, ultrapure water, ammonia, and anhydrous ethanol is (35-45) mg: 18 mL: 150 μL: 8 mL.

[0017] Furthermore, in step S3 of the present invention, the ratio of dopamine hydrochloride to ultrapure water is 100 mg: 1 mL, and the mass ratio of dopamine hydrochloride to BPNSs is (49-51): 4.

[0018] Furthermore, in step S4 of this invention, the ratio of manganese nitrate to ultrapure water is 10 mg:1 mL, and the mass ratio of manganese nitrate to dopamine hydrochloride is 1:10.

[0019] Furthermore, in step S5 of the present invention, the ratio of ascorbic acid to ultrapure water is 10 mg: 1 mL, and the mass ratio of ascorbic acid to dopamine hydrochloride is 1: 10.

[0020] Furthermore, in step S5 of the present invention, ultrapure water is used for resuspension, and the centrifugation speed is 4000 r / min.

[0021] Furthermore, in step S6 of the present invention, the mass concentration of the PCL aqueous solution is 8%, and the ratio of BPNSs-PDA@Mn3O4, hexafluoroisopropanol, and PCL aqueous solution is 10mg:1mL:100mg; the stirring speed during stirring is 300r / min; and the ultrasonic power during ultrasonic treatment is 120W.

[0022] Furthermore, in step S6 of the present invention, the receiving speed of the electrospinning machine is 100 r / min, the voltage is 15 kV, the spraying speed is 0.6 mL / h, the distance between the nozzle and the collector is 10 cm, and the environmental conditions are a temperature of 25°C and a humidity of 30-50%.

[0023] Another technical problem to be solved by the present invention is to provide a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane prepared by the above preparation method.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1) This invention encapsulates PDA on the surface of BPNSs and Mn3O4, which can effectively reduce the cytotoxicity of Mn3O4, so that the prepared BPNSs-PDA@Mn3O4 has the characteristics of BPNSs, PDA and Mn3O4. At the same time, BPNSs-PDA@Mn3O4 is introduced into PCL, and PCL-BPNSs-PDA@Mn3O4 nanofiber membrane with wound healing ability is prepared by electrospinning.

[0026] 2) The black phosphorus nanosheets (BPNSs) used in this invention have good cell differentiation induction properties, mechanical properties, biocompatibility and low cytotoxicity, thus effectively improving the mechanical properties, cell compatibility and wound healing ability of PCL-BPNSs-PDA@Mn3O4 nanofiber membrane.

[0027] 3) The dopamine-coated PDA used in this invention has strong adhesion ability and can form a uniform, dense and controllable film on the surface of BPNSs and Mn3O4, which enhances the adhesion of the material and improves its surface properties. At the same time, it has good biocompatibility, which reduces inflammatory response and cytotoxicity when the PCL-BPNSs-PDA@Mn3O4 nanofiber membrane comes into contact with organisms.

[0028] 4) The manganese tetroxide (Mn3O4), dopamine coating, and black phosphorus nanosheets (BPNSs) in the PCL-BPNSs-PDA@Mn3O4 nanofiber membrane of the present invention all have strong photothermal properties. The aromatic ring and phenolic groups in the dopamine molecule can absorb light energy and convert it into heat energy. Mn3O4 can absorb light in the wavelength range of visible light and near-infrared light (NIR) to produce a photothermal effect. Therefore, the PCL-BPNSs-PDA@Mn3O4 nanofiber membrane has potential application value in photothermal therapy and photothermal conversion. Attached Figure Description

[0029] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, do not constitute an undue limitation of the invention. In the drawings:

[0030] Figure 1 This is a diagram showing the results of a cell scratch experiment in Example 1 of the present invention. Detailed Implementation

[0031] The present invention will now be described in detail with reference to specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0032] Example 1

[0033] PCL-BPNSs-PDA@Mn3O4 nanofiber membranes were prepared according to the following steps:

[0034] S1. Black phosphorus powder was dispersed in N-methylpyrrolidone at a ratio of 10 mg: 1 mL. The mixture was placed in an ice bath under light-protected conditions and sonicated at 200 W for 8 hours using an ultrasonic cell disruptor. Then, the mixture was centrifuged at 3000 r / min for 8 min to obtain the supernatant. The supernatant was then centrifuged at 12000 r / min for 10 min to obtain the precipitate. The precipitate was washed three times with anhydrous ethanol to remove N-methylpyrrolidone and obtain a black phosphorus dispersion. The black phosphorus dispersion was then vacuum dried to constant weight to obtain black phosphorus nanosheets (BPNSs).

[0035] S2. Add the BPNSs obtained in step S1 to ultrapure water and sonicate for 3 min to obtain a uniformly dispersed BPNSs suspension. Add 20% ammonia and anhydrous ethanol to the BPNSs suspension and stir magnetically for 25 min to obtain mixture one. The ratio of BPNSs, ultrapure water, ammonia and anhydrous ethanol is 40 mg: 18 mL: 150 μL: 8 mL.

[0036] S3. Dissolve dopamine hydrochloride in ultrapure water to obtain a dopamine solution. The ratio of dopamine hydrochloride to ultrapure water is 100 mg: 1 mL. Add the dopamine solution dropwise to the first mixture obtained in step S2. The mass ratio of dopamine hydrochloride to BPNSs is 50: 4. Stir for 24 h to obtain the second mixture.

[0037] S4. Dissolve manganese nitrate in ultrapure water to obtain manganese nitrate solution. The ratio of manganese nitrate to ultrapure water is 10 mg: 1 mL. Add the manganese nitrate solution dropwise to the mixture obtained in step S3. The mass ratio of manganese nitrate to dopamine hydrochloride is 1:10. Stir magnetically for 6 hours to obtain mixture three.

[0038] S5. Dissolve ascorbic acid in ultrapure water to obtain an ascorbic acid solution. The ratio of ascorbic acid to ultrapure water is 10 mg: 1 mL. Add the ascorbic acid solution dropwise to the mixture obtained in step S4. The mass ratio of ascorbic acid to dopamine hydrochloride is 1:10. After stirring for 6 hours, centrifuge for 10 minutes. Discard the supernatant and resuspend the precipitate in ultrapure water. Centrifuge twice in the same way to obtain the precipitate. The centrifugation speed is 4000 r / min. Vacuum dry the precipitate to constant weight to obtain BPNSs-PDA@Mn3O4.

[0039] S6. Add the BPNSs-PDA@Mn3O4 obtained in step S5 to hexafluoroisopropanol, stir at 300 r / min for 12 h, then sonicate at 120 W for 30 min, and then add an 8% (w / w) PCL aqueous solution. The ratio of BPNSs-PDA@Mn3O4, hexafluoroisopropanol, and PCL aqueous solution is 10 mg: 1 mL: 100 mg. Stir at 300 r / min for 12 h, then sonicate at 120 W. An electrospinning solution was obtained after 30 minutes. The electrospinning solution was placed on the syringe of an electrospinning machine for electrospinning to obtain a fiber membrane. The fiber membrane was then dried in a vacuum drying oven to constant weight to obtain a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane. The receiving speed of the electrospinning machine was 100 r / min, the voltage was 15 kV, the spraying speed was 0.6 mL / h, the distance between the nozzle and the collector was 10 cm, and the environmental conditions were a temperature of 25℃ and a humidity of 30-50%.

[0040] Example 2

[0041] PCL-BPNSs-PDA@Mn3O4 nanofiber membranes were prepared according to the following steps:

[0042] S1. Black phosphorus powder was dispersed in N-methylpyrrolidone at a ratio of 10 mg: 1 mL. The mixture was placed in an ice bath under light-protected conditions and sonicated at 200 W ultrasonic power for 10 hours using an ultrasonic cell disruptor. Then, the mixture was centrifuged for 10 min at 3000 r / min to obtain the supernatant. The supernatant was then centrifuged for 10 min at 12000 r / min to obtain the precipitate. The precipitate was washed three times with anhydrous ethanol to remove N-methylpyrrolidone and obtain a black phosphorus dispersion. The black phosphorus dispersion was then vacuum dried to constant weight to obtain black phosphorus nanosheets (BPNSs).

[0043] S2. Add the BPNSs obtained in step S1 to ultrapure water and sonicate for 4 min to obtain a uniformly dispersed BPNSs suspension. Add 20% ammonia and anhydrous ethanol to the BPNSs suspension and stir magnetically for 30 min to obtain mixture one. The ratio of BPNSs, ultrapure water, ammonia and anhydrous ethanol is 35 mg: 18 mL: 150 μL: 8 mL.

[0044] S3. Dissolve dopamine hydrochloride in ultrapure water to obtain a dopamine solution. The ratio of dopamine hydrochloride to ultrapure water is 100 mg: 1 mL. Add the dopamine solution dropwise to the first mixture obtained in step S2. The mass ratio of dopamine hydrochloride to BPNSs is 49: 4. Stir for 20 h to obtain the second mixture.

[0045] S4. Dissolve manganese nitrate in ultrapure water to obtain manganese nitrate solution. The ratio of manganese nitrate to ultrapure water is 10 mg: 1 mL. Add the manganese nitrate solution dropwise to the mixture obtained in step S3. The mass ratio of manganese nitrate to dopamine hydrochloride is 1:10. Stir magnetically for 12 h to obtain mixture three.

[0046] S5. Dissolve ascorbic acid in ultrapure water to obtain an ascorbic acid solution. The ratio of ascorbic acid to ultrapure water is 10 mg: 1 mL. Add the ascorbic acid solution dropwise to the mixture obtained in step S4. The mass ratio of ascorbic acid to dopamine hydrochloride is 1:10. After stirring for 12 h, centrifuge for 10 min. Discard the supernatant and resuspend the precipitate in ultrapure water. Centrifuge three times in the same way to obtain the precipitate. The centrifugation speed is 4000 r / min. Vacuum dry the precipitate to constant weight to obtain BPNSs-PDA@Mn3O4.

[0047] S6. Add the BPNSs-PDA@Mn3O4 obtained in step S5 to hexafluoroisopropanol, stir at 300 r / min for 10 h, then sonicate at 120 W for 25 min, and then add an 8% (w / w) PCL aqueous solution. The ratio of BPNSs-PDA@Mn3O4, hexafluoroisopropanol, and PCL aqueous solution is 10 mg: 1 mL: 100 mg. Stir at 300 r / min for 10 h, then sonicate at 120 W. An electrospinning solution was obtained after 25 minutes. The electrospinning solution was placed on the syringe of an electrospinning machine for electrospinning to obtain a fiber membrane. The fiber membrane was then dried in a vacuum drying oven to constant weight to obtain a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane. The receiving speed of the electrospinning machine was 100 r / min, the voltage was 15 kV, the spraying speed was 0.6 mL / h, the distance between the nozzle and the collector was 10 cm, and the environmental conditions were a temperature of 25℃ and a humidity of 30-50%.

[0048] Example 3

[0049] PCL-BPNSs-PDA@Mn3O4 nanofiber membranes were prepared according to the following steps:

[0050] S1. Black phosphorus powder was dispersed in N-methylpyrrolidone at a ratio of 10 mg: 1 mL. The mixture was placed in an ice bath under light-protected conditions and sonicated at 200 W for 12 hours using an ultrasonic cell disruptor. Then, the mixture was centrifuged at 3000 r / min for 12 min to obtain the supernatant. The supernatant was then centrifuged at 12000 r / min for 10 min to obtain the precipitate. The precipitate was washed three times with anhydrous ethanol to remove N-methylpyrrolidone and obtain a black phosphorus dispersion. The black phosphorus dispersion was then vacuum dried to constant weight to obtain black phosphorus nanosheets (BPNSs).

[0051] S2. Add the BPNSs obtained in step S1 to ultrapure water and sonicate for 5 min to obtain a uniformly dispersed BPNSs suspension. Add 20% ammonia and anhydrous ethanol to the BPNSs suspension and stir magnetically for 35 min to obtain mixture one. The ratio of BPNSs, ultrapure water, ammonia and anhydrous ethanol is 45 mg: 18 mL: 150 μL: 8 mL.

[0052] S3. Dissolve dopamine hydrochloride in ultrapure water to obtain a dopamine solution. The ratio of dopamine hydrochloride to ultrapure water is 100 mg: 1 mL. Add the dopamine solution dropwise to the first mixture obtained in step S2. The mass ratio of dopamine hydrochloride to BPNSs is 51: 4. Stir for 21 h to obtain the second mixture.

[0053] S4. Dissolve manganese nitrate in ultrapure water to obtain manganese nitrate solution. The ratio of manganese nitrate to ultrapure water is 10 mg: 1 mL. Add the manganese nitrate solution dropwise to the mixture obtained in step S3. The mass ratio of manganese nitrate to dopamine hydrochloride is 1:10. Stir magnetically for 24 h to obtain mixture three.

[0054] S5. Dissolve ascorbic acid in ultrapure water to obtain an ascorbic acid solution. The ratio of ascorbic acid to ultrapure water is 10 mg: 1 mL. Add the ascorbic acid solution dropwise to the mixture obtained in step S4. The mass ratio of ascorbic acid to dopamine hydrochloride is 1:10. After stirring for 24 h, centrifuge for 10 min. Discard the supernatant and resuspend the precipitate in ultrapure water. Centrifuge 4 times in the same way to obtain the precipitate. The centrifugation speed is 4000 r / min. Vacuum dry the precipitate to constant weight to obtain BPNSs-PDA@Mn3O4.

[0055] S6. Add the BPNSs-PDA@Mn3O4 obtained in step S5 to hexafluoroisopropanol, stir at 300 r / min for 15 h, then sonicate at 120 W for 35 min, and then add an 8% (w / w) PCL aqueous solution. The ratio of BPNSs-PDA@Mn3O4, hexafluoroisopropanol, and PCL aqueous solution is 10 mg: 1 mL: 100 mg. Stir at 300 r / min for 15 h, then sonicate at 120 W. An electrospinning solution was obtained after 35 minutes. The electrospinning solution was placed on the syringe of an electrospinning machine for electrospinning to obtain a fiber membrane. The fiber membrane was then dried in a vacuum drying oven to constant weight to obtain a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane. The receiving speed of the electrospinning machine was 100 r / min, the voltage was 15 kV, the spraying speed was 0.6 mL / h, the distance between the nozzle and the collector was 10 cm, and the environmental conditions were 25℃ and 30-50% humidity.

[0056] Comparative Example: The difference from Example 1 is that steps S2-6 are not included, and BPNSs that are not encapsulated by PDA and do not contain Mn3O4 are obtained.

[0057] Experimental example: Cell scratch test

[0058] 5×10 per hole 5 L929 cells were seeded into 6-well plates at a predetermined cell density. After 24 hours, the cells adhered to the plates. Once the plates were confluent, a sterile 20 μL pipette tip was used to create cell scratches on the bottom of the plates perpendicular to the wells. The old culture medium was removed, and the plates were washed three times with sterile PBS to remove the scratched cells, making the remaining spaces clearly visible to the naked eye. The culture medium was then replaced with serum-free medium, and two groups were prepared: one group was treated with BPNSs, and the other group was treated with BPNSs-PDA@Mn3O4 prepared in Example 1. Observations and photographs were taken under a microscope at 0, 24, and 48 hours. Figure 1 As shown, the wound healing distance between scratches in cells treated with BPNSs-PDA@Mn3O4 is better than that in the control group, indicating that BPNSs-PDA@Mn3O4 can promote the migration ability of L929 cells. It also indirectly shows that the PCL-BPNSs-PDA@Mn3O4 prepared in this invention has the ability to promote wound healing.

[0059] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A method for preparing a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane, characterized in that: Includes the following steps: S1. Disperse black phosphorus powder in N-methylpyrrolidone, place in an ice bath under light-protected conditions, and sonicate using an ultrasonic cell disruptor for 8-12 hours. Then, centrifuge for 8-12 minutes to obtain the supernatant. Centrifuge the supernatant for 10 minutes to obtain the precipitate. Wash the precipitate three times with anhydrous ethanol to remove N-methylpyrrolidone to obtain a black phosphorus dispersion. Vacuum dry the black phosphorus dispersion to constant weight to obtain black phosphorus nanosheets (BPNSs). The ratio of black phosphorus powder to N-methylpyrrolidone is 10 mg: 1 mL. The ultrasonic power of the ultrasonic cell disruptor is 200 W. The centrifugation speed for the first centrifugation is 3000 r / min. The centrifugation speed for the second centrifugation is 12000 r / min. S2. Add the BPNSs obtained in step S1 to ultrapure water and sonicate for 3-5 minutes to obtain a uniformly dispersed BPNSs suspension. Add ammonia and anhydrous ethanol to the BPNSs suspension and stir magnetically for 25-35 minutes to obtain mixture one. The mass concentration of ammonia is 20%, and the ratio of BPNSs, ultrapure water, ammonia, and anhydrous ethanol is (35-45) mg: 18 mL: 150 μL: 8 mL. S3. Dissolve dopamine hydrochloride in ultrapure water to obtain a dopamine solution. Add the dopamine solution dropwise to the first mixture obtained in step S2 and stir for 20-24 hours to obtain the second mixture. The ratio of dopamine hydrochloride to ultrapure water is 100 mg: 1 mL, and the mass ratio of dopamine hydrochloride to BPNSs is (49-51):

4. S4. Dissolve manganese nitrate in ultrapure water to obtain manganese nitrate solution. Add manganese nitrate solution dropwise to mixture two obtained in step S3 and stir magnetically for 6-24 hours to obtain mixture three. The ratio of manganese nitrate to ultrapure water is 10 mg: 1 mL, and the mass ratio of manganese nitrate to dopamine hydrochloride is 1:

10. S5. Dissolve ascorbic acid in ultrapure water to obtain an ascorbic acid solution. Add the ascorbic acid solution dropwise to the mixture obtained in step S4. Stir for 6-24 hours, centrifuge for 10 minutes, discard the supernatant, and resuspend the precipitate. Centrifuge 2-4 times in the same way to obtain the precipitate. Dry the precipitate under vacuum to constant weight to obtain BPNSs-PDA@Mn3O4. The ratio of ascorbic acid to ultrapure water is 10 mg: 1 mL, and the mass ratio of ascorbic acid to dopamine hydrochloride is 1:

10. S6. Add the BPNSs-PDA@Mn3O4 obtained in step S5 to hexafluoroisopropanol, stir for 10-15 h, sonicate for 25-35 min, add PCL aqueous solution, stir for 10-15 h, sonicate for 25-35 min to obtain electrospinning solution, place the electrospinning solution on the syringe of an electrospinning machine for electrospinning to obtain fiber membrane, place the fiber membrane in a vacuum drying oven to dry to constant weight to obtain PCL-BPNSs-PDA@Mn3O4 nanofiber membrane; the mass concentration of PCL aqueous solution is 8%, the ratio of BPNSs-PDA@Mn3O4, hexafluoroisopropanol, and PCL aqueous solution is 10 mg: 1 mL: 100 mg; the stirring speed is 300 r / min; the ultrasonic power is 120 W during ultrasonic treatment.

2. The method for preparing a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane according to claim 1, characterized in that: In step S5, ultrapure water is used for resuspension, and the centrifugation speed is 4000 r / min.

3. The method for preparing a PCL-BPNSs-PDA@Mn3O4 nanofiber membrane according to claim 1, characterized in that: In step S6, the receiving speed of the electrospinning machine is 100 r / min, the voltage is 15 kV, the spraying speed is 0.6 mL / h, the distance between the nozzle and the collector is 10 cm, and the ambient temperature is 25℃ and the humidity is 30-50%.

4. The PCL-BPNSs-PDA@Mn3O4 nanofiber membrane prepared by the preparation method according to any one of claims 1 to 3.

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