A Pediococcus acidilactici strain A21186 with anti-aging and anti-stress functions and its application

Abstract

The present invention provides a lactic acid Pediococcus strain A21186 with anti-aging and anti-stress functions and its application, belonging to the field of microbial technology. The present invention isolates lactic acid Pediococcus strain A21186 from feces of healthy and long-lived elderly people, has excellent tolerance in artificial gastrointestinal fluid and bile salts, and at the same time, the strain A21186 can improve the health index of nematodes, enhance the movement ability of nematodes, delay the influence of aging on their movement to a certain extent, and enhance the resistance of nematodes to external stimuli, and can be used as a potential probiotic for the preparation of medicines with anti-aging and / or antioxidant properties.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to a Pediococcus acidilactici strain A21186 with anti-aging and anti-stress functions and an application thereof. Background Art

[0002] Aging is a complex physiological phenomenon, a physiological process of behavioral defects and functional disorders that occur with age. During the aging process, the adaptability of organisms to the external environment gradually decreases. At present, aging and aging-related diseases have become one of the focuses of research. The intestinal flora plays an important role in metabolic and immune regulation. Studies have shown that the intestinal microbiome changes with age and changes in age-related diseases. At the same time, it also regulates age-related damage to the host body. Therefore, screening and isolating probiotic strains with anti-aging and antioxidant effects from intestinal microorganisms is of great significance to the development of the probiotic industry.

[0003] Probiotics are active microorganisms that contribute to the health of the host after taking an appropriate amount. They have functions such as immune regulation, promoting nutrient absorption and resisting the growth and reproduction of harmful bacteria. Common probiotics include Bifidobacterium, Lactobacillus and Lactaseibacillus. Pediococcus acidilactici belongs to the genus Pediococcus and is mainly found in fermented plant materials and pickled vegetables. It is a group of microorganisms that live in the body and are beneficial to the health of the host. It has important functions in inhibiting fat accumulation and antagonizing pathogenic microorganisms, but there are few reports on its anti-aging and anti-stress effects. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a novel strain of Pediococcus acidilactici, which has the effects of delaying aging and / or enhancing anti-stress ability.

[0005] The invention provides a Pediococcus acidilactici strain A21186, whose deposit number is GDMCC No:63976.

[0006] The invention provides a microbial agent with the functions of delaying aging and / or resisting stress, comprising the Pediococcus acidilactici strain A21186 and auxiliary materials.

[0007] Preferably, the effective viable count of the lactic acid Pediococcus strain A21186 is (1-100)×10 7 CFU / mL.

[0008] The present invention provides a method for culturing the Pediococcus acidilactici strain A21186, comprising the following steps:

[0009] The activated Pediococcus acidilactici strain A21186 was inoculated into MRS medium for culture.

[0010] Preferably, the culture temperature is 36-38°C;

[0011] The culture time is 45 to 50 hours.

[0012] Preferably, the inoculation amount is 3% to 5%.

[0013] The present invention provides use of the Pediococcus acidilactici strain A21186, the microbial agent or the Pediococcus acidilactici strain A21186 obtained by the culture method in preparing products that delay aging and / or resist stress.

[0014] Preferably, the delaying of aging includes extending life span and enhancing athletic ability.

[0015] Preferably, the anti-stress ability includes anti-oxidative stress or anti-heat stress ability.

[0016] Preferably, the product is a medicine in oral dosage form.

[0017] The present invention provides a Pediococcus acidilactici strain A21186, which has a deposit number of GDMCC No: 63976. The strain A21186 is separated from the feces of healthy and long-lived elderly people, has excellent tolerance in artificial gastrointestinal fluid and bile salts, and is expected to pass through the intestine and colonize in the intestine. The present invention uses Caenorhabditis elegans as a model organism to evaluate the biological function of the strain A21186. The results show that the present invention screens out Pediococcus acidilactici A21186, which can improve the health indicators of nematodes, enhance the motility of nematodes, delay the influence of aging on its movement to a certain extent, and enhance the resistance of nematodes to external stimuli. It can be used as a potential probiotic in the fields of medicines. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The colony morphology (a) and Gram staining (b) of Pediococcus acidilactici A21186 in Example 1;

[0019] Figure 2 is the phylogenetic tree of Pediococcus acidilactici A21186 and A21017;

[0020] Figure 3 The viable bacterial count (1g CFU / mL) of A21186 at different bile salt concentrations in Example 2;

[0021] Figure 4 The comparison results of the effects of Pediococcus acidilactici A21186 and A21017 on the life span of nematodes in Example 3;

[0022] Figure 5 The results of the effect of the Pediococcus acidilactici A21186 strain on the movement index of nematodes in Example 4;

[0023] Figure 6 The results of the effect of the Pediococcus acidilactici A21186 strain on heat stress in nematodes in Example 5;

[0024] Figure 7 These are the results of the effect of Pediococcus acidilactici A21186 strain on H2O2-induced oxidative stress in Example 6.

[0025] Biomaterial Deposit Information

[0026] Pediococcus acidilactici strain A21186 is deposited in Guangdong Microbiological Culture Collection Center, abbreviated as GDMCC, with the address at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Institute of Microbiology, the deposit date is November 1, 2023, and the deposit number is GDMCC No: 63976. DETAILED DESCRIPTION

[0027] The invention provides a Pediococcus acidilactici strain A21186, whose preservation number is GDMCC No:63976.

[0028] In the present invention, strain A21186 is separated from the feces of healthy and long-lived elderly people, and its morphological characteristics on MRS culture medium are as follows: colony morphological characteristics: small colonies with white protrusions, round opaque, smooth surface and neat edges. The bacterial morphology of the Pediococcus acidilactici A21186 obtained after separation and purification is observed and the cell morphology is observed after Gram staining. The Gram staining of the strain is purple, and the cells are spherical and non-motile. At the same time, the present invention also carries out molecular identification based on 16S rDNA, and the 16SrDNA sequence of strain A21186 is compared in the NCBI database. The result shows that the gene sequence of strain A21186 is the highest with Pediococcus acidilactici, which is 99.93%. Therefore, based on the results of morphological identification and molecular identification, strain A21186 is identified as Pediococcus acidilactici.

[0029] In the present invention, the strain A21186 was tested for its resistance to artificial gastrointestinal fluid and bile salts. The results showed that after the strain A21186 was treated with simulated gastric fluid for 3 hours, the logarithmic value of live bacteria reached 8, and the survival rate of the strain was 220.00%, indicating that the strain A21186 could continue to grow in the simulated gastric fluid; after being treated with simulated intestinal fluid for 8 hours, the survival rate of the strain was 96.98%, and the logarithmic value of live bacteria reached above 7, indicating that the strain A21186 had a strong survival ability in the simulated gastrointestinal fluid, and had obvious advantages over other lactic acid Pediococcus strains screened in the same batch, and was expected to colonize in the intestine.

[0030] The invention provides a method for culturing the Pediococcus acidilactici strain A21186, comprising the following steps: inoculating the activated Pediococcus acidilactici strain A21186 into an MRS culture medium for culturing.

[0031] In the present invention, the culture temperature is preferably 36-38°C, more preferably 37°C. The culture time is preferably 45-50h, more preferably 48h. The inoculation amount of the inoculation is preferably 3%-5%, more preferably 4%. The activation method of the lactic acid Pediococcus strain A21186 is preferably to inoculate the lactic acid Pediococcus strain A21186 into MRS solid culture medium and culture at 37°C for 24h. The culture is preferably cultured under anaerobic conditions.

[0032] The invention provides a microbial agent with the functions of delaying aging and / or resisting stress, comprising the Pediococcus acidilactici strain A21186 and auxiliary materials.

[0033] In the present invention, the preparation method of the microbial agent is preferably to expand the culture of the lactic acid Pediococcus strain A21186, collect the bacterial liquid and mix it with glycerol to obtain a microbial agent aqueous solution; or separate the bacteria from the collected bacterial liquid, mix the bacteria and a lyophilization protective agent, and freeze-dry to obtain a lyophilized powder of the microbial agent. The added volume percentage of the glycerol is 40% to 60%, and more preferably 50%. The present invention does not impose any special restrictions on the type of the lyophilization protective agent, and the type of lyophilization protective agent known in the art can be used, such as one or more of sucrose, trehalose, gelatin, and lactoprotein. When the microbial agent is a microbial agent aqueous solution, the effective viable count of the lactic acid Pediococcus strain A21186 is preferably (1 to 100)×10 7 CFU / mL, more preferably 5 to 80×10 7 CFU / mL, the most preferred is 10×10 7 When the microbial agent is a lyophilized powder of the microbial agent, the effective viable count of the lactic acid Pediococcus strain A21186 is preferably (1 to 100)×10 7 CFU / g, more preferably 5 to 80×10 7CFU / g, the most preferred is 10×10 7 CFU / g.

[0034] The present invention provides use of the Pediococcus acidilactici strain A21186, the microbial agent or the Pediococcus acidilactici strain A21186 obtained by the culture method in preparing products that delay aging and / or resist stress.

[0035] In the present invention, the delay of aging preferably includes extending life span and / or enhancing athletic ability. The anti-stress ability preferably includes anti-oxidative stress or anti-heat stress ability.

[0036] In one embodiment of the present invention, the effect of strain A21186 in delaying aging was evaluated using the model organism Caenorhabditis elegans as the object. Caenorhabditis elegans has the characteristics of simple individual structure, short life cycle, easy cultivation, and low experimental cost. The experiment shows that strain A21186 and lactic acid Pediococcus strain A21017 screened in the same batch can prolong the life span of Caenorhabditis elegans. Compared with the control group (OP50), the median life span of nematodes fed with strains A21186 and A21017 was extended by 16.13% and 9.68%, respectively. The present invention also evaluates the effect of strain A21186 on the motor ability of nematodes by measuring the number of body bending times and head swinging frequency of Caenorhabditis elegans. The results show that as the life span of nematodes increases, the corresponding body bending frequency and head swinging frequency of nematodes are reduced. Compared with the control group (OP50), the body bending frequency of nematodes fed with A21186 within 30s on the 10th and 15th days increased by 31.74% and 54.63%, respectively. The results showed that strain A21186 can enhance the movement ability of nematodes, improve their muscle function, and alleviate the impact of aging on the movement of nematodes to a certain extent.

[0037] In another embodiment of the present invention, the effect of strain A21186 in stress resistance was evaluated with the model organism Caenorhabditis elegans as the object. The present invention evaluates its ability to resist oxidative stress by measuring the survival curve and survival rate of nematodes in the presence of hydrogen peroxide. The results show that strain A21186 can effectively reduce damage under oxidative stress and enhance resistance to external stimuli; while nematodes fed with strain A21017 have almost no resistance to 100mM H2O2. In addition, the present invention also studies the effect of strain A21186 on nematode resistance to heat stress. The nematodes are cultured at 37°C and their survival curves and survival rates are measured. The results show that compared with the control group (OP50), the survival rate of nematodes fed with A21186 to heat stress is increased by more than 33.33%, while the life span of nematodes fed with strain A21017 in a 37°C environment is increased by more than 16.67% compared with the control group. This indicates that strain A21186 has excellent ability to improve nematodes' resistance to heat stress.

[0038] In the present invention, in view of the excellent tolerance of the strain A21186 to artificial gastrointestinal fluid and bile salts and the potential intestinal colonization ability, the product is preferably an oral dosage form of medicine. The present invention has no particular limitation on the preparation method of the product, and the preparation method of the microorganism-containing product known in the art can be used.

[0039] The following is a detailed description of the anti-aging and anti-stress Pediococcus acidilactici strain A21186 and its application provided by the present invention in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0040] Example 1

[0041] Screening and identification of Pediococcus acidilactici A21186

[0042] 1. Strain Isolation and Purification

[0043] The stool samples of centenarians in Guangxi were diluted 10-fold. -3 ~10 -6 0.1 mL of each sample dilution was spread on MRS solid medium and cultured in an anaerobic workstation at 37°C for 48 h. The target strain was selected according to the colony morphology of Pediococcus, and the strain was purified by plate streaking. The growth morphology of Pediococcus acidilactici A21186 on MRS plate is as follows: Figure 1 As shown in (a). Colony morphology: small colonies with white protrusions, round opaque, smooth surface and neat edges. The bacterial morphology of the isolated and purified Pediococcus acidilactici A21186 was observed, as well as the cell morphology after Gram staining. Figure 1 Middle (b) shows that the strain is Gram-stained purple, and the cells are spherical and non-motile.

[0044] The MRS solid culture medium comprises: 10 g of peptone, 5 g of beef extract powder, 4 g of yeast extract powder, 20 g of glucose, 2 g of dipotassium hydrogen phosphate, 2 g of triammonium citrate, 5 g of sodium acetate, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate, 15 g of agar, and 1 g of Tween-80. After adding 1 L of deionized water, the pH value is adjusted to 6.2±0.2, and sterilized at 121° C. for 15 min.

[0045] 2. Strain 16S rDNA

[0046]

[0047] Figure 2 shown.

[0048] Pediococcus acidilactici A21017 is a Pediococcus acidilactici strain screened in the same batch of experiments, and is used as a control strain in the specific examples.

[0049] Example 2

[0050] Test on resistance of Pediococcus acidilactici to artificial gastrointestinal fluid and bile salt

[0051] 1. Experiment on resistance of Pediococcus acidilactici to artificial gastric juice

[0052] The activated strains (A21186 and A21017) were cultured overnight and the bacterial solution was diluted to an OD 600 The value is about 1, and 10% of the total amount is added to artificial gastric juice (pH 3.0), and cultured at 37°C with shaking. The artificial gastric juice after 0h, 1h and 3h of culture is taken for plate count of viable bacteria. The 0h is used as the control, and the survival rate is calculated according to formula (1).

[0053] Survival rate of artificial gastric juice resistance (%) = N t / N0*100% formula (1)

[0054] Where N t N represents the number of viable bacteria after culture for th, and N0 represents the number of viable bacteria at 0h.

[0055] 2. Experiment on resistance of Pediococcus acidilactici to artificial intestinal fluid

[0056] The artificial gastric juice after 3 hours of culture was added to the artificial intestinal juice (pH 8.0) at a rate of 10%, and cultured at 37°C with shaking. The artificial intestinal juice at 0 hours, 2 hours, 4 hours, 6 hours and 8 hours was taken for plate viable bacteria count. The survival rate was calculated according to formula (2) with 0 hours as the control.

[0057] Survival rate against artificial intestinal fluid (%) = N t / N0*100% formula (2)

[0058] Where N t N represents the number of viable bacteria after culture for th, and N0 represents the number of viable bacteria at 0h.

[0059] The results are shown in Table 1.

[0060] Table 1 Tolerance of A21186 and A21017 in simulated artificial gastrointestinal fluid

[0061]

[0062] As shown in Table 1, after 3 hours of treatment with simulated gastric juice, the live bacteria logarithm value of strain A21186 reached 8, and the survival rate of the strain was 220.00%, indicating that strain A21186 can continue to grow in simulated gastric juice; after 8 hours of treatment with simulated intestinal juice, the survival rate of the strain was 96.98%, and the live bacteria logarithm value reached more than 7, indicating that strain A21186 has strong survival ability in simulated gastrointestinal juice. Compared with the control strain A21017, under the same experimental conditions, the live bacteria count of strain A21186 is larger, indicating that strain A21186 is more tolerant than A21017 in simulated artificial gastrointestinal juice.

[0063] 3. Bile salt tolerance test of Pediococcus acidilactici

[0064] The activated strains (A21186 and A21017) were cultured overnight, centrifuged at 10000 r / min for 10 min, washed three times with PBS, diluted to an OD600 value of 1, and inoculated into MRS liquid culture medium containing 0.1%, 0.2% and 0.3% ox bile salts at 10% concentration, cultured at 37°C with shaking, and the bacterial liquids at 0h, 1h, 2h, 3h and 4h were taken for plate count of live bacteria. The 0h was used as a control, and the survival rate was calculated according to formula (3).

[0065] Bile salt tolerance survival rate (%) = N t / N0×100% formula (3)

[0066] Where N t N represents the number of viable bacteria after culture for th, and N0 represents the number of viable bacteria at 0h.

[0067] Results Figure 3 and Table 2.

[0068] Table 2 Tolerance of A21186 to bile salts

[0069]

[0070] Depend on Figure 3 As shown in Table 2, after being treated with 0.1%, 0.2% and 0.3% ox bile salt solution for 4 hours, the survival rates of strain A21186 were 135.89%, 68.33% and 23.17%, respectively, indicating that strain A21186 has good tolerance to bile salts. In this experiment, after three independent repeated experiments, it was found that strain A21017 had very poor tolerance in MRS liquid culture medium with 0.1%, 0.2% and 0.3% ox bile salt. There were two cases in which no colonies were found in the counting plate after being cultured in MRS liquid culture medium with 0.2% and 0.3% ox bile salt. Therefore, no specific relevant data are listed.

[0071] In summary, Pediococcus acidilactici A21186 has stronger tolerance to simulated artificial gastrointestinal fluid and bile salts than A21017, can effectively resist the influence of gastrointestinal fluid and bile salts, has the potential to maintain a high survival rate while passing through the human gastrointestinal tract, and is expected to colonize in the intestine.

[0072] Example 3

[0073] Effects of strains on lifespan of nematodes

[0074] 1. Strain activation and preparation of NGM plates

[0075] The activated strains (A21186 and A21017) and the control strain (Escherichia coli OP50) were inoculated into MRS liquid culture medium and LB liquid culture medium at 1% respectively, and cultured in a shaking incubator at 37°C overnight. 100 μL of the cultured bacterial liquid was spread on NGM culture medium respectively.

[0076] 2. Synchronization of Nematodes

[0077] Use M9 buffer to suspend the Caenorhabditis elegans adults in the NGM plate, transfer the suspension to about 3.5 mL of the centrifuge tube, add lysis buffer (0.5 mL of 5M NaOH and 1 mL of 5% sodium hypochlorite solution), immediately vortex and lyse for 6 minutes, centrifuge at 3500r / min for 1 minute, discard the supernatant, wash repeatedly with M9 buffer 4 times, discard the supernatant, transfer to NGM culture medium, and culture at 25°C overnight to obtain L1 larvae; rinse the L1 nematodes with M9 buffer and collect them in EP tubes, centrifuge at 3500r / min for 1 minute to remove the supernatant, transfer the nematodes to the NGM plate containing OP50, and culture at 25°C for 28 to 30 hours to obtain L4 nematodes.

[0078] 3. Nematode lifespan experiment

[0079] The L4 stage nematodes were transferred to NGM medium containing OP50, A21186 and A21017, 3 plates per group, 50 nematodes per plate, and cultured at 20°C. The day when the nematodes were picked was recorded as day 0. The plates were changed every 2 days and the number of deaths was counted until all nematodes died. Nematodes that died abnormally were not counted. Survival curves were calculated using GraphPad Prism 5.

[0080] Among them, the strains screened were Pediococcus acidilactici A21186 and A21017. Feeding the strains A21186 and A21017 prolonged the lifespan of nematodes. Figure 4 The median lifespans of nematodes fed with strains OP50, A21186, and A21017 were 15.5 days, 18 days, and 17 days, respectively. Compared with the control, the median lifespans of nematodes fed with strains A21186 and A21017 were extended by 16.13% and 9.68%, respectively.

[0081] Example 4

[0082] Effects of strains on nematode motility

[0083] 1. Body bending measurement

[0084] The L4 nematodes were transferred to NGM plates containing OP50 and A21186, with 30 nematodes per group. The day of picking the nematodes was recorded as day 0, and the nematodes were cultured at 20°C for 5, 10, and 15 days. The nematodes were transferred one by one to a blank NGM plate, and after adapting for 30 seconds, the number of sinusoidal curves generated within 30 seconds was counted under a microscope. The results are shown in Figure 5 Middle (A).

[0085] 2. Head shaking frequency measurement

[0086] As with the body bending experiment, the nematodes were cultured at 20°C for 5, 10, and 15 days, and then transferred one by one to NGM plates containing M9 buffer. After acclimation for 30 seconds, the frequency of head swings of the nematodes within 30 seconds was counted one by one. One swing of the head from one side to the other and then back to the original position was counted. The results are shown in Figure 5 (B).

[0087] Depend on Figure 5 It can be seen that the body bending frequency and head swinging frequency of nematodes in the control group and A21186 group decreased with the increase of lifespan. Compared with the control group, the nematodes fed with strain A21186 had stronger locomotion ability under the same time. Compared with the control group, the body bending frequency of nematodes fed with A21186 within 30 seconds increased by 31.74% and 54.63% on the 10th and 15th days, respectively, indicating that strain A21186 can enhance the locomotion ability of nematodes, improve their muscle function, and alleviate the impact of aging on nematode locomotion to a certain extent.

[0088] Example 5

[0089] Effects of strains on nematode resistance to heat stress

[0090] The L4 stage nematodes were transferred to NGM plates coated with OP50, A21186 and A21017 strains and cultured at 20°C for 5 days. The plates were transferred to a 37°C incubator. This time was recorded as 0h (survival rate was 100%), and the number of nematode deaths was recorded every 1h to draw a survival curve. The experiment was repeated three times independently, and the results are shown in Tables 3 and Figure 6 shown.

[0091] As shown in Table 3, the average lifespan of nematodes fed with OP50, strain A21186 and A21017 at 37°C was 6h, 8h and 7h respectively. Compared with the control group (nematodes fed with OP50), the survival rate of nematodes fed with A21186 to heat stress increased by more than 33.33%, indicating that feeding strain A21186 can improve the ability of nematodes to resist heat stress. The lifespan of nematodes fed with strain A21017 in a 37°C environment increased by more than 16.67% compared with the control group. It can be seen that both lactic acid bacteria A21186 and A21017 can improve the resistance of nematodes to 37°C stressors.

[0092] Table 3 Effects of A21186 and A21017 on nematode resistance to heat stress

[0093]

[0094]

[0095] Example 6

[0096] Effects of strains on nematode resistance to oxidative stress

[0097] Take 300uL 100 mM H2O2 solution and evenly spread it on a blank NGM plate. After the plate is dry, add 20uL of OP50, A21186 and A21017 bacterial solution respectively. Transfer the L4 nematodes to the NGM plates coated with OP50, A21186 and A21017 strains and culture them at 20℃ for 5 days. Expose the nematodes to the NGM plates containing H2O2 and bacterial solution, record the number of nematode deaths every 1h, and draw a survival curve. The results are shown in Figure 7 The experiment was repeated 3 times, and the results are shown in Table 4.

[0098] The average life span of nematodes fed with OP50 and A21186 in 100mM H2O2 was 5h and 6h respectively. Compared with the control group, the survival rate of nematodes fed with A21186 to 100mM H2O2 oxidative stress increased by 20%, indicating that strain A21186 can effectively reduce damage under oxidative stress and enhance resistance to external stimuli. Nematodes fed with strain A21017 had almost no resistance to 100mM H2O2.

[0099] Table 4 Effects of A21186 and A21017 on oxidative stress in nematodes

[0100]

[0101] It can be seen from the results of the above examples that A21186 has excellent life extension and stress resistance capabilities, and A21186 has stronger tolerance in the simulated gastrointestinal environment, can survive, and has the potential to colonize in the gastrointestinal tract of the body and exert its effects for a long time.

[0102] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Pediococcus acidilactici strain A21186, characterized in that: The deposit number is GDMCC No:63976.

2. A microbial agent having the function of delaying aging and / or resisting stress, characterized in that: The invention comprises the Pediococcus acidilactici strain A21186 as claimed in claim 1 and auxiliary materials.

3. The microbial agent according to claim 2, characterized in that: The effective viable count of the lactic acid Pediococcus strain A21186 is (1-100)×10 7 CFU / mL.

4. A method for culturing the Pediococcus acidilactici strain A21186 according to claim 1, characterized in that: The following steps are involved: The activated Pediococcus acidilactici strain A21186 was inoculated into MRS medium for culture.

5. The culture method according to claim 4, characterized in that: The culture temperature is 36-38°C; The culture time is 45 to 50 hours.

6. The culture method according to claim 4, characterized in that: The inoculation amount of the inoculation is 3% to 5%.

7. Use of the Pediococcus acidilactici strain A21186 according to claim 1, the microbial agent according to claim 2 or 3, or the Pediococcus acidilactici strain A21186 obtained by the culture method according to claim 4 or 5 in the preparation of medicines for delaying aging and / or resisting stress.

8. The use according to claim 7, characterized in that: The delayed aging includes extending lifespan and / or enhancing athletic ability.

9. The use according to claim 7, characterized in that: The anti-stress ability includes anti-oxidative stress or anti-heat stress ability.

Images