A delivery system and method for packed bed carriers in column chromatography

Abstract

The application relates to a conveying system of a packing carrier in column chromatography, which comprises a storage device for storing the packing carrier, a first conveying device, a plurality of chromatographic columns, a second conveying device, a drying device and a gas-solid separation device. The feeding port of the first conveying device is connected with the first discharging port of the storage device through a pipeline. The plurality of chromatographic columns are connected in parallel through pipelines. The first feeding port of each chromatographic column is connected with the discharging port of the first conveying device through a pipeline. The feeding port of the second conveying device is connected with the first discharging port of each chromatographic column through a pipeline. The feeding port of the drying device is connected with the discharging port of the second conveying device through a pipeline. The discharging port of the drying device is connected with the feeding port of the storage device through a pipeline. The feeding port of the gas-solid separation device is connected with the second discharging port of the storage device through a pipeline. The first discharging port of the gas-solid separation device is connected with the third feeding port of each chromatographic column through a pipeline. The conveying system and the conveying method can reduce physical labor and realize full-closed operation.

Description

Technical Field

[0001] This invention relates to the field of column chromatography technology, and in particular to a transport system and method for transporting a packing medium in column chromatography. Background Technology

[0002] As an adsorbent material in column chromatography separation and purification, the current recycling methods for packing carriers mostly involve manually operated equipment, such as single silica gel columns, series combined columns, or parallel combined columns. This involves heavy physical labor such as handling and loading / unloading. Moreover, the non-fully enclosed operation of the above-mentioned equipment cannot meet occupational health requirements, causing occupational injuries to operators and harming the production environment.

[0003] Therefore, there is an urgent need for a transport system and method for the packing carrier in column chromatography. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a transport system and method for packing carriers in column chromatography.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] A first aspect of the present invention is to provide a delivery system for a packed carrier in column chromatography, comprising:

[0007] Storage device for storing the filling carrier;

[0008] A first conveying device, wherein the inlet of the first conveying device is connected to the first outlet pipe of the storage device;

[0009] A plurality of chromatography columns are connected in parallel via pipelines. The first inlet of each chromatography column is connected to the outlet pipeline of the first conveying device, the second inlet of each chromatography column is connected to the outlet pipeline of the upstream section, and the second outlet of each chromatography column is connected to the inlet pipeline of the downstream section.

[0010] The second conveying device has its inlet connected to the first outlet pipe of each of the chromatography columns.

[0011] A drying device, wherein the inlet of the drying device is connected to the outlet pipe of the second conveying device, and the outlet of the drying device is connected to the inlet pipe of the storage device;

[0012] The gas-solid separation device includes an inlet connected to the second outlet pipe of the storage device, a first outlet connected to the third inlet pipe of each chromatography column, and a second outlet connected to the inlet pipe of the waste section.

[0013] Preferably, the filling carrier includes at least one of silica gel, activated carbon, or porous polymer microspheres.

[0014] Preferably, the elution solution comprises at least one of ethanol, petroleum ether, or n-hexane.

[0015] Preferably, both the first conveying device and the second conveying device are auger conveyors.

[0016] Preferably, the drying device is a ribbon dryer.

[0017] Preferably, the gas-solid separation device is a cyclone separator.

[0018] A second aspect of the present invention is to provide a method for transporting a packed carrier in column chromatography, employing the transport system described above, comprising the following steps:

[0019] S1. The packing carrier is transported from the storage device to the chromatography column via the first conveying device;

[0020] S2, The upstream section supplies raw materials and elution solution to the chromatography column;

[0021] After washing,

[0022] The chromatography column delivers the eluted product to the downstream section;

[0023] S3. The loading carrier is transported from the chromatography column to the drying device via the second conveying device; simultaneously, the gas-solid separation device supplies inert gas to the chromatography column.

[0024] S4. The dried loading carrier is transported from the drying device to the storage device by the pressure provided by the inert gas; at this time, the small-sized loading carrier is carried by the inert gas to the gas-solid separation device.

[0025] S5. Remove the small-sized packing carrier from the gas-solid separation device.

[0026] The present invention adopts the above technical solution and has the following technical effects compared with the prior art:

[0027] The conveying system and conveying method of the present invention can realize automatic control of the recycling of the filling carrier, which greatly reduces physical labor. The fully enclosed operation can meet occupational health requirements and avoid harm to employees and the environment. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the conveying system in this invention;

[0029] Figure 2 This is a schematic diagram of the conveying method in this invention;

[0030] The reference numerals in the figures include:

[0031] Storage device 1; chromatography column 2; drying device 3; gas-solid separation device 4; first conveying device 51; second conveying device 52. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0034] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the scope of the invention.

[0035] Example 1

[0036] like Figure 1 As shown, this embodiment provides a transport system for packing carriers in column chromatography, comprising:

[0037] Storage device 1, the storage device 1 being used to store the filling carrier;

[0038] The first conveying device 51 is connected to the first discharge port of the storage device 1 via a pipeline.

[0039] A plurality of chromatography columns 2 are connected in parallel via pipelines. The first inlet of each chromatography column 2 is connected to the outlet pipeline of the first conveying device 51, the second inlet of each chromatography column 2 is connected to the outlet pipeline of the upstream section, and the second outlet of each chromatography column 2 is connected to the inlet pipeline of the downstream section.

[0040] The second conveying device 52 is connected to the first outlet pipeline of each of the chromatography columns 2.

[0041] The drying device 3 has its inlet connected to the outlet pipe of the second conveying device 52, and its outlet connected to the inlet pipe of the storage device 1.

[0042] And a gas-solid separation device 4, wherein the inlet of the gas-solid separation device 4 is connected to the second outlet pipeline of the storage device 1, the first outlet of the gas-solid separation device 4 is connected to the third inlet pipeline of each of the chromatography columns 2, and the second outlet of the gas-solid separation device 4 is connected to the inlet pipeline of the waste section.

[0043] In a preferred embodiment, the filling carrier includes at least one of silica gel, activated carbon, or porous polymer microspheres.

[0044] In a preferred embodiment, the elution solution includes at least one of ethanol, petroleum ether, or n-hexane.

[0045] In a preferred embodiment, both the first conveying device 51 and the second conveying device 52 are screw conveyors.

[0046] In a preferred embodiment, the drying device 3 is a ribbon dryer.

[0047] In a preferred embodiment, the gas-solid separation device 4 is a cyclone separator.

[0048] Example 2

[0049] This embodiment provides a method for transporting packed carriers in column chromatography, using the transport system described in Embodiment 1, and the steps include:

[0050] S1. The silica gel is transported from the storage device 1 to the chromatography column 2 by the first screw conveyor 51;

[0051] S2, The upstream section supplies raw materials and n-hexane to the chromatography column 2;

[0052] After washing,

[0053] The chromatography column 2 delivers the eluted product to the downstream section;

[0054] S3. The silica gel is conveyed from the chromatography column 2 to the ribbon dryer 3 by the second screw conveyor 52; at the same time, the cyclone separator 4 delivers nitrogen gas to the chromatography column 2 to pressurize the silica gel, thereby improving the conveying efficiency of the silica gel and preventing the silica gel from adhering to the inner wall of the pipeline, thus preventing pipeline blockage.

[0055] S4. The dried silica gel is conveyed from the ribbon dryer 3 to the storage device 1 by the pressure provided by the nitrogen gas; at this time, the silica gel with small particle size is carried by the nitrogen gas to the cyclone separator 4.

[0056] Optionally, S5, the silica gel with small particle size is periodically removed from the cyclone separator 4.

[0057] Example 3

[0058] This embodiment provides a method for transporting packed carriers in column chromatography, using the transport system described in Embodiment 1, and the steps include:

[0059] S1. Activated carbon is transported from the storage device 1 to the chromatography column 2 by the first screw conveyor 51;

[0060] S2, The upstream section supplies raw materials and ethanol to the chromatography column 2;

[0061] After washing,

[0062] The chromatography column 2 delivers the eluted product to the downstream section;

[0063] S3. The activated carbon is conveyed from the chromatography column 2 to the ribbon dryer 3 by the second screw conveyor 52; at the same time, the cyclone separator 4 delivers nitrogen gas to the chromatography column 2 to pressurize the activated carbon, thereby improving the conveying efficiency of the activated carbon and preventing the activated carbon from adhering to the inner wall of the pipeline, thus preventing pipeline blockage.

[0064] S4. The dried activated carbon is conveyed from the screw dryer 3 to the storage device 1 by the pressure provided by the nitrogen gas; at this time, the activated carbon with small particle size is carried by the nitrogen gas to the cyclone separator 4.

[0065] Optionally, S5, the activated carbon with small particle size is periodically removed from the cyclone separator 4.

[0066] Example 4

[0067] This embodiment provides a method for transporting packed carriers in column chromatography, using the transport system described in Embodiment 1, and the steps include:

[0068] S1. The polymer porous microspheres (i.e. macroporous resin) are transported from the storage device 1 to the chromatography column 2 by the first screw conveyor 51.

[0069] S2, The upstream section supplies raw materials and n-hexane to the chromatography column 2;

[0070] After washing,

[0071] The chromatography column 2 delivers the eluted product to the downstream section;

[0072] S3. The polymer porous microspheres are conveyed from the chromatography column 2 to the ribbon dryer 3 by the second screw conveyor 52; at the same time, the cyclone separator 4 delivers nitrogen gas to the chromatography column 2 to pressurize the polymer porous microspheres, thereby improving the conveying efficiency of the polymer porous microspheres and preventing the polymer porous microspheres from adhering to the inner wall of the pipeline, thus preventing pipeline blockage.

[0073] S4. The dried polymer porous microspheres are transported from the ribbon dryer 3 to the storage device 1 by the pressure provided by the nitrogen gas; at this time, the tiny polymer porous microspheres are carried by the nitrogen gas to the cyclone separator 4.

[0074] Optionally, S5, the small-sized porous polymer microspheres are periodically removed from the cyclone separator 4.

[0075] In summary, the conveying system and conveying method of the present invention can realize automatic control of the recycling of the filling carrier, greatly reducing physical labor, and the fully enclosed operation can meet occupational health requirements while avoiding harm to employees and the environment.

[0076] The above description is merely a preferred embodiment of the present invention and does not limit the implementation and protection scope of the present invention. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present invention should be included within the protection scope of the present invention.

Claims

1. A delivery system for a packed carrier in column chromatography, characterized in that, include: Storage device (1), the storage device (1) being used to store the filling carrier; The first conveying device (51) has its inlet connected to the first outlet pipe of the storage device (1). A plurality of chromatography columns (2) are connected in parallel by pipelines. The first inlet of each chromatography column (2) is connected to the outlet pipeline of the first conveying device (51). The second inlet of each chromatography column (2) is connected to the outlet pipeline of the upstream section. The upstream section conveys raw materials and elution solution to the chromatography column (2). The second outlet of each chromatography column (2) is connected to the inlet pipeline of the downstream section. The chromatography column (2) conveys eluted products to the downstream section. The inlet of the second conveying device (52) is connected to the first outlet pipeline of each of the chromatography columns (2); The drying device (3) has its inlet connected to the outlet pipe of the second conveying device (52) and its outlet connected to the inlet pipe of the storage device (1). And a gas-solid separation device (4), the inlet of the gas-solid separation device (4) is connected to the second outlet pipe of the storage device (1), the first outlet of the gas-solid separation device (4) is connected to the third inlet pipe of each of the chromatography columns (2), the gas-solid separation device (4) supplies inert gas to the chromatography column (2), and the second outlet of the gas-solid separation device (4) is connected to the inlet pipe of the waste section.

2. The conveying system according to claim 1, characterized in that, The filling carrier includes at least one of silica gel, activated carbon, or porous polymer microspheres.

3. The conveying system according to claim 1, characterized in that, The elution solution includes at least one of ethanol, petroleum ether, or n-hexane.

4. The conveying system according to claim 1, characterized in that, Both the first conveying device (51) and the second conveying device (52) are screw conveyors.

5. The conveying system according to claim 1, characterized in that, The drying device (3) is a ribbon dryer.

6. The conveying system according to claim 1, characterized in that, The gas-solid separation device (4) is a cyclone separator.

7. A method for transporting a packed carrier in column chromatography, employing the transport system as described in any one of claims 1-6, characterized in that the steps include... include: S1. The loading carrier is transported from the storage device (1) to the chromatography column (2) via the first conveying device (51). S2, The upstream section supplies raw materials and elution solution to the chromatography column (2); After washing, The chromatography column (2) delivers the eluted product to the downstream section; S3. The loading carrier is transported from the chromatography column (2) to the drying device (3) through the second conveying device (52); at the same time, the gas-solid separation device (4) delivers inert gas to the chromatography column (2); S4. The dried loading carrier is transported from the drying device (3) to the storage device (1) by the pressure provided by the inert gas; at this time, the small-sized loading carrier is carried by the inert gas to the gas-solid separation device (4). S5. Remove the small-sized packing carrier from the gas-solid separation device (4).

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