Composition containing dietary fiber and preparation method thereof
By adding components such as probiotics and L-carnitine to dietary fiber foods, we can regulate the intestinal flora and develop a dietary fiber composition that can achieve healthy, safe, nutritious and continuous weight loss effects.
Patent Information
- Application Number
- CN202410324057.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-21
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2044-03-21
AI Technical Summary
It is difficult to achieve both health, safety, nutrition and continuous weight loss effects on existing dietary fiber foods.
Develop a composition containing dietary fiber, which can regulate the diversity of intestinal bacteria, enhance metabolism, reduce blood lipid levels and achieve weight loss effects through the synergistic effect of probiotics, dietary fiber, L-carnitine and other components.
The composition is rich in a variety of nutrients, can enrich the diversity of intestinal flora, enhance metabolic levels, prevent diseases, regulate blood lipid levels, and has a good weight loss effect.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of food, and in particular to a composition containing dietary fiber and a preparation method thereof. Background Art
[0002] Dietary fiber is a polysaccharide that is not digested and absorbed in the human mouth, stomach and small intestine. China's national food safety standard "GB / Z 21922-2008 Basic Terminology of Food Nutrients" defines dietary fiber as: polymers of carbohydrates naturally present, extracted or synthesized in plants, with a degree of polymerization DP ≥ 3, which cannot be digested and absorbed by the human small intestine and have health significance to the human body. Dietary fiber can play a role by changing the properties of gastrointestinal contents and changing the absorption mode of other nutrients and chemicals. It has a certain regulatory effect in lowering blood sugar, lowering blood lipids, fighting cancer, improving intestinal flora, and improving constipation.
[0003] Short-term intake of too little or no dietary fiber can cause constipation; long-term intake of too little fiber will increase the risk of cardiovascular disease, intestinal disease, and type 2 diabetes. Long-term low-fiber diets, especially for adolescents (less than 15g / d), will increase the risk of chronic diseases several times after the age of 40.
[0004] Obesity increases the risk of developing a variety of chronic diseases and promotes the development of these diseases, including type 2 diabetes, hypertension, non-alcoholic fatty liver disease, cardiovascular disease, mental illness, tumors, etc. Losing weight by eating dietary fiber is still a popular way of losing weight, and various healthy and nutritious meals are constantly being developed.
[0005] Chinese patent CN110292168A discloses a meal replacement composition and its preparation method and application. The raw materials of the meal replacement composition include chicken breast paste and edible fungus-derived chitosan oligosaccharide. The mass ratio of the edible fungus-derived chitosan oligosaccharide to the chicken breast paste is ≥0.03:1. The meal replacement composition may further include auxiliary materials such as shredded chicken, egg white, dietary fiber and seasoning. The composition mainly achieves the purpose of weight loss by enhancing satiety, reversibly reducing gastric void capacity, prolonging gastric emptying and reducing food intake. However, using the composition as a meal replacement for weight loss may lead to malnutrition due to the lack of other nutrients required by the human body.
[0006] Chinese patent application CN112006277A discloses a composition with a weight loss effect, a preparation method thereof, and a health product, including nutritional ingredients, a first functional ingredient, a second functional ingredient, and an energy supplement ingredient, wherein the nutritional ingredients include soy protein isolate and skim milk powder, the first functional ingredient includes resistant dextrin, kelp dietary fiber, psyllium husk powder, inulin, and chia seeds; the second functional ingredient includes kelp extract, and the energy supplement ingredient includes jujube powder and black sesame. The composition provides a sense of fullness and promotes fat decomposition to achieve a weight loss effect. However, the composition achieves a slow weight loss effect.
[0007] Chinese patent CN112915208A discloses a composition and application with the function of improving the diversity of personalized intestinal flora. The composition includes spleen-strengthening and qi-replenishing ingredients, dampness-removing and blood-activating ingredients, and intestinal bacteria energy substrates. Through the bidirectional regulatory effect on the intestinal flora, the composition improves and maintains the diversity of personalized intestinal flora, and plays a therapeutic role in metabolic or disease. However, due to the large differences in dietary habits among different people in my country, the flora in different regions of the human body is quite different, so it may only play a regulatory role for some people, and microorganisms will develop resistance, and the effect may weaken over time.
[0008] Therefore, it is still necessary to develop dietary fiber foods that are healthy, safe, nutritious, and have good sustained weight loss effects. Summary of the invention
[0009] The purpose of the present invention is to provide a composition containing dietary fiber and a preparation method thereof. The composition contains a variety of nutrients required by the human body, and through the synergistic effect of various components such as probiotics, dietary fiber, L-carnitine, etc., the diversity of intestinal flora is regulated, metabolism is enhanced, blood lipid levels are reduced, and a weight-loss effect is exerted.
[0010] To achieve the above object, the present invention provides a composition containing dietary fiber, comprising the following components in parts by weight:
[0011] 55-85 parts of dietary fiber, 0.1-7 parts of probiotics, 1-9 parts of red ginseng extract, 0.01-2 parts of phytosterols, 0.01-0.2 parts of L-carnitine, 0.05-0.8 parts of multivitamins and 0.05-0.1 parts of mineral elements;
[0012] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, and the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is (1-3):(1-2):(0.5-1):(0.5-1):(0.5-1);
[0013] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, wherein the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is (5-20):(3-15):(1-5), and the viable bacteria concentration of the probiotics is 6×10 8 CFU / g-1×10 11 CFU / g.
[0014] The dietary fiber includes water-soluble dietary fiber or water-insoluble dietary fiber.
[0015] Furthermore, the method for extracting laver fiber comprises the following steps:
[0016] S1, wash the laver, simmer at a high pressure of 0.2-0.8 MPa for 1-3 min, cool, filter, and mash;
[0017] S2, dispersing the crushed laver crumbs with 5-10 times of water, adding 0.1-1.0‰ of protease, enzymolyzing in a water bath at 25-37° C. for 6-24 hours, centrifuging, and immersing the centrifugal residue in ethanol water for 2-3 times, each time for 5-10 minutes, wherein the mass ratio of ethanol to water is (1-2):(1-2), to obtain laver residue;
[0018] S3, dispersing the laver residues in S2 with 5-10 times of water, adding 0.1-1.0‰ of cellulase, enzymolyzing in a water bath at 25-37°C for 12-24h, filtering, freeze-drying, and grinding into powder to obtain the laver fiber.
[0019] Furthermore, the composition also contains 25-40 parts of whey protein, 10-20 parts of fish maw oligopeptide and 20-40 parts of soy protein.
[0020] Preferably, the complex cellulose contains at least two of vitamin A, vitamin C, vitamin D, vitamin E, vitamin K1, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, pantothenic acid and biotin.
[0021] More preferably, the complex cellulose contains at least vitamin C, vitamin E and pantothenic acid.
[0022] More preferably, the mass ratio of vitamin C, vitamin E and pantothenic acid is (1-2):(1-2):(1-2).
[0023] Preferably, the mineral elements include one or more of iron, zinc, calcium, magnesium and selenium.
[0024] A method for preparing a composition containing dietary fiber comprises the following steps: weighing various raw materials according to a formula, and mixing them evenly to obtain the composition.
[0025] A nutritional preparation comprises the above-mentioned composition containing dietary fiber and auxiliary materials.
[0026] The invention discloses an application of a composition containing dietary fiber in preparing fat-reducing food or health-care product.
[0027] The structure of the body's intestinal flora is greatly affected by dietary factors. People who choose different dietary combinations have different intestinal flora numbers and structures. Through reasonable dietary combinations, increasing dietary fiber intake and enhancing the activity of the beneficial intestinal flora, health can be improved and disease prevention can be achieved with half the effort.
[0028] Adding soluble dietary fiber will decompose it by intestinal microorganisms. The fructan in its decomposition product is a proliferation factor for beneficial intestinal bacteria and can effectively promote the proliferation of bifidobacteria. The polydextrose produced by the decomposition can improve the intestinal microecological environment, promote the growth of beneficial bacteria such as lactobacillus and bifidobacterium, and inhibit the growth of pathogenic bacteria. The short-chain fatty acids produced by fermentation have a regulating effect on the intestinal pH value, can inhibit the formation of amines, phenolic compounds, etc., and antagonize the growth of pathogenic bacteria such as Escherichia coli and Staphylococcus aureus.
[0029] The present invention has the beneficial effects:
[0030] The dietary fiber-containing composition provided by the present invention is rich in various nutritional elements, such as dietary fiber, probiotics, protein, red ginseng extract, phytosterols, L-carnitine, complex vitamins and mineral elements, etc., which can supplement various required nutrients for the human body, enhance immunity, enrich the diversity of intestinal flora, enhance metabolic level, prevent diseases, regulate blood lipid levels, and have a good weight loss effect.
[0031] The dietary fiber provided by the present invention comprises laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, which are extracted from various types of fibers from seafood, cereals, grains and fruits, respectively, and dietary fibers from different sources may have different metabolic and physiological effects due to their different physicochemical properties. The probiotics provided by the present invention comprise bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, which indirectly shape the intestinal flora and change the diversity of the intestinal flora by regulating the composition of the probiotics, which can activate beneficial bacteria in the body, inhibit harmful bacteria, and affect the metabolism and immune system of the host.
[0032] The experiment of the present invention proves that the components such as dietary fiber, probiotics, red ginseng extract, phytosterols, L-carnitine and the like can work in a coordinated manner to jointly enhance the metabolic regulation effect, reduce blood lipid levels, inhibit fat and body weight gain, and have a good weight loss effect. DETAILED DESCRIPTION
[0033] The specific implementation methods of the technical solution of this specification are further described below through specific examples. These examples are for the purpose of describing the technical solution in detail, rather than for limiting the technical solution. Based on the examples in this specification, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this specification.
[0034] In the specification of the present invention and the following examples, conventional instruments and equipment in the art are used. The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. The various raw materials used in the following examples are conventional commercial products unless otherwise specified, and their specifications are conventional specifications in the art.
[0035] Example 1
[0036] A composition containing dietary fiber, comprising the following components in parts by weight:
[0037] 70 parts of dietary fiber, 0.5 parts of probiotics, 1 part of red ginseng extract, 1 part of phytosterols, 0.05 parts of L-carnitine, 0.03 parts of vitamin C, 0.02 parts of vitamin E, 0.02 parts of pantothenic acid, 0.02 parts of iron, 0.02 parts of zinc, 0.03 parts of calcium and 0.02 parts of magnesium.
[0038] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, and the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is 3:2:1:1:1.
[0039] The method for extracting laver fiber comprises the following steps:
[0040] S1, wash the laver, simmer at a high pressure of 0.5 MPa for 2 min, cool, filter, and mash;
[0041] S2, dispersing the crushed laver crumbs with 10 times of water, adding 0.5‰ protease, enzymolyzing in a 35°C water bath for 8 hours, centrifuging, and immersing the centrifugal residue in ethanol water for 3 times, each time for 10 minutes, wherein the mass ratio of ethanol to water is 2:1, to obtain laver residue;
[0042] S3, dispersing the laver residues in S2 with 10 times of water, adding 0.5‰ of cellulase, enzymolyzing in a water bath at 35°C for 12 hours, filtering, freeze-drying, and grinding into powder to obtain the laver fiber.
[0043] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is 15:10:3, and the viable count of the probiotics is 1×1010 CFU / g.
[0044] Weigh all the raw materials according to the formula, mix them evenly, and you have the product.
[0045] Example 2
[0046] A composition containing dietary fiber, comprising the following components in parts by weight:
[0047] 55 parts of dietary fiber, 0.3 parts of probiotics, 1 part of red ginseng extract, 0.5 parts of phytosterols, 0.03 parts of L-carnitine, 0.02 parts of vitamin C, 0.02 parts of vitamin E, 0.02 parts of pantothenic acid, 0.02 parts of iron, 0.02 parts of zinc, 0.03 parts of calcium and 0.01 parts of magnesium.
[0048] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is 2:2:0.8:1:1, and the preparation method of the laver fiber is the same as that of Example 1.
[0049] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, wherein the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is 15:15:5, and the viable count of the probiotics is 5×10 10 CFU / g.
[0050] Weigh all the raw materials according to the formula, mix them evenly, and you have the product.
[0051] Example 3
[0052] A composition containing dietary fiber, comprising the following components in parts by weight:
[0053] 85 parts of dietary fiber, 1 part of probiotics, 1.5 parts of red ginseng extract, 0.05 parts of phytosterols, 0.05 parts of L-carnitine, 0.03 parts of vitamin C, 0.02 parts of vitamin E, 0.02 parts of pantothenic acid, 0.02 parts of iron, 0.02 parts of zinc, 0.03 parts of calcium and 0.02 parts of magnesium.
[0054] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is 1:1:1:1:1, and the preparation method of the laver fiber is the same as that of Example 1.
[0055] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, wherein the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is 15:10:5, and the viable count of the probiotics is 9×108 CFU / g.
[0056] Weigh all the raw materials according to the formula, mix them evenly, and you have the product.
[0057] Example 4
[0058] A composition containing dietary fiber, comprising the following components in parts by weight:
[0059] 40 parts of whey protein, 10 parts of fish maw oligopeptide, 25 parts of soy protein, 75 parts of dietary fiber, 3 parts of probiotics, 5 parts of red ginseng extract, 0.4 parts of phytosterols, 0.07 parts of L-carnitine, 0.03 parts of vitamin C, 0.02 parts of vitamin E, 0.02 parts of pantothenic acid, 0.02 parts of iron, 0.02 parts of zinc, 0.03 parts of calcium and 0.02 parts of magnesium.
[0060] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is 3:2:0.5:0.5:1, and the preparation method of the laver fiber is the same as that of Example 1.
[0061] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is 5:3:5, and the viable count of the probiotics is 1×10 11 CFU / g.
[0062] Weigh all the raw materials according to the formula, mix them evenly, and you have the product.
[0063] Example 5
[0064] A composition containing dietary fiber, comprising the following components in parts by weight:
[0065] 30 parts of whey protein, 15 parts of fish maw oligopeptides, 40 parts of soy protein, 85 parts of dietary fiber, 1 part of probiotics, 2 parts of red ginseng extract, 0.05 parts of phytosterols, 0.02 parts of L-carnitine, 0.03 parts of vitamin C, 0.02 parts of vitamin E, 0.02 parts of pantothenic acid, 0.02 parts of iron, 0.02 parts of zinc, 0.03 parts of calcium and 0.02 parts of magnesium.
[0066] The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is 2:2:1:1:0.5, and the preparation method of the laver fiber is the same as that of Example 1.
[0067] The probiotics are a mixture of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus, wherein the mass ratio of bifidobacterium longum, lactobacillus rhamnosus and lactobacillus acidophilus is 10:10:3, and the viable count of the probiotics is 2×10 9 CFU / g.
[0068] Weigh all the raw materials according to the formula, mix them evenly, and you have the product.
[0069] Comparative Example 1
[0070] The difference from Example 1 is that dietary fiber is not contained.
[0071] Comparative Example 2
[0072] The difference from Example 1 is that no probiotics are contained.
[0073] Comparative Example 3
[0074] The difference from Example 1 is that red ginseng extract, L-carnitine and phytosterols are not contained.
[0075] Comparative Example 4
[0076] The difference from Example 1 is that the dietary fiber is oat fiber.
[0077] Comparative Example 5
[0078] The difference from Example 1 is that the dietary fiber is oat fiber and does not contain probiotics.
[0079] Comparative Example 6
[0080] The difference from Example 1 is that the dietary fiber is oat fiber, the probiotic is Lactobacillus longum, and the viable cell count concentration is 1×10 5 CFU / g.
[0081] Comparative Example 7
[0082] The difference from Example 1 is that the dietary fiber is soybean fiber.
[0083] Comparative Example 8
[0084] The difference from Example 1 is that laver fiber is not contained.
[0085] Test Example 1
[0086] 1.1 Experimental design and methods
[0087] 130 SPF SD rats, 6-7 weeks old, weighing 80-90g, were randomly divided into two groups after adaptive feeding of basic feed for 7 days: normal group (10 rats) and high-fat modeling group (120 rats), with half males and half females in each group. The normal group was fed with basic feed (93.5% rice, 5% fish meal, 1% cereal flour, 0.5 salt), and the high-fat modeling group was fed with nutritious high-fat feed (80% basic feed + 15% lard + 2% cholesterol + 3% egg yolk). The rats were weighed once a week for 10 weeks. When the obesity degree is greater than 20%, it is considered obese, indicating that the obesity model has been successfully established.
[0088] After the modeling was completed, 110 rats with successful modeling were selected and randomly divided into 10 groups, 10 in each group. The groups were divided into: model group (normal saline water 6.0 mL / kg / day), group A (composition of embodiment 1, 6 g / kg / day), group B (composition of embodiment 4, 6 g / kg / day), group C (composition of comparative example 1, 6 g / kg / day), group D (composition of comparative example 2, 6 g / kg / day), group E (composition of comparative example 3, 6 g / kg / day), group F (composition of comparative example 4, 6 g / kg / day), group G (composition of comparative example 5, 6 g / kg / day), group H (composition of comparative example 6, 6 g / kg / day), group I (composition of comparative example 7, 6 g / kg / day), group J (composition of comparative example 8, 6 g / kg / day), and food and water were randomly provided. At the same time, the above-mentioned normal group was used as a blank control group, and the blank control group was intragastrically gavaged with normal saline (6.0 mL / kg / day) every day for 10 consecutive weeks.
[0089] After 10 weeks, all mice were fasted overnight, weighed, and then killed by cervical dislocation to collect blood samples. The mice were dissected and the weights of the liver, heart, kidney, spleen, epididymal fat, abdominal fat, and perirenal fat were measured.
[0090] 1.2 Test indicators
[0091] The measured indicators include weight gain, fat weight, and blood lipid levels (TC, TG, LDL-C, HDL-C). The blood biochemical index levels were measured according to the kit instructions.
[0092] 1.3 Experimental Results
[0093] 1.3.1 General growth conditions
[0094] During the experiment, the rats in the control group were active, had a normal diet, and had neat fur. The rats in the high-fat model group were lethargic and had yellowish fur. After 10 weeks, the animals showed varying degrees of lethargy, decreased activity, and messy fur. Throughout the experiment, the weight of the rats in the high-fat model group was always higher than that of the rats in other groups. At the end of the experiment, all rats in each group survived.
[0095] 1.3.2 Weight gain and fat weight
[0096] From the test results in Table 1, it can be seen that the body weight and fat weight of mice in the blank control group were significantly lower than those in the model group, and there was a significant difference (p<0.01); compared with the model group, the body weight and fat weight of mice in each feeding group of the nutritional composition group were reduced to varying degrees (p<0.05 or p<0.01).
[0097] Table 1 Effects of the composition on body weight and fat weight of rats (mean ± SD, n = 10)
[0098]
[0099]
[0100] Note: # indicates that compared with the blank control group, the difference is significant p<0.05, and the difference is extremely significant p<0.01; * indicates that compared with the model group, the difference is significant p<0.05, and the difference is extremely significant p<0.01.
[0101] The weight gain and fat weight of rats fed with the nutritional compositions containing dietary fiber of Examples 1 and 4 were significantly lower than those fed with the compositions of Comparative Examples 1-7, and had better weight loss effects. Comparative Examples 1-7 are the control groups of Example 1: Compared with Example 1, the composition of Comparative Example 1 does not contain dietary fiber, and probiotics and other components play a certain weight loss effect together, and the weight gain and fat weight of rats are lower than those of the modeling group, but the weight loss effect is worse than that of Example 1; Compared with Example 1, the composition of Comparative Example 2 does not contain probiotics, and dietary fiber and other components play a certain weight loss effect together, and the weight gain and fat weight of rats are lower than those of the modeling group, but the weight loss effect is worse than that of Example 1; Compared with Example 1, Comparative Example 3 does not contain red ginseng extract, L-carnitine and phytosterols, and dietary fiber and probiotics play a good weight loss effect together, and the weight gain and fat weight of rats are lower than those of the modeling group, but the weight loss effect is worse than that of Example 1; Compared with Example 1, the ingredients of dietary fiber in Comparative Examples 4-8 are different, and the weight loss effect is significantly worse. It can be seen that the components of the dietary fiber-containing nutritional composition provided by the present invention can coordinately enhance the weight loss effect on rats, especially dietary fiber, probiotics, red ginseng extract, phytosterols, and L-carnitine coordinately inhibit the increase of weight and fat in rats. Therefore, the dietary fiber component provided by the present invention has a better weight loss effect.
[0102] 1.3.3 Serum lipid biochemical indicators (TC, TG, LDL-C, HDL-C)
[0103] According to the instructions of the kit, the levels of low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), thyroglobulin (TG) and total cholesterol (TC) in rats were detected.
[0104] Table 2 Effects of the composition on various serum biochemical indices of rats (mean ± SD, n = 10)
[0105] Group TC(mmol / L) TG (mmol / L) LDL-C (mmol / L) HDL-C (mmol / L) Blank control group 1.46±0.13 0.47±0.03 0.26±0.04 0.92±0.10 Model Group <![CDATA[3.10±0.57 ## ]]> <![CDATA[1.49±0.09 ## ]]> <![CDATA[0.99±0.11 # ]]> <![CDATA[0.51±0.06 # ]]> Group A (Example 1) <![CDATA[1.79±0.13 * ]]> <![CDATA[0.86±0.04 * ]]> <![CDATA[0.37±0.06 * ]]> 0.88±0.07 Group B (Example 4) <![CDATA[1.65±0.13 * ]]> <![CDATA[0.75±0.035 * ]]> <![CDATA[0.32±0.04 * ]]> <![CDATA[0.93±0.06 * ]]> Group C (Comparative Example 1) 2.13±0.19 1.09±0.08 0.84±0.04 0.69±0.08 Group D (Comparative Example 2) 2.20±0.12 1.13±0.06 0.80±0.07 0.61±0.05 Group E (Comparative Example 3) 2.56±0.25 1.29±0.10 0.89±0.05 0.59±0.02 Group F (Comparative Example 4) <![CDATA[1.98±0.19 * ]]> 0.88±0.07 0.72±0.04 0.73±0.03 Group G (Comparative Example 5) 2.77±0.18 1.32±0.06 0.91±0.06 0.58±0.05 Group H (Comparative Example 6) 2.62±0.20 1.30±0.03 0.86±0.05 0.65±0.07 Group I (Comparative Example 7) 2.05±0.15 0.97±0.09 0.78±0.04 0.70±0.03 Group J (Comparative Example 7) 2.09±0.13 1.05±0.07 0.87±0.03 0.61±0.04
[0106] Note: # indicates that compared with the blank control group, the difference is significant p<0.05, and the difference is extremely significant p<0.01; * indicates that compared with the model group, the difference is significant p<0.05, and the difference is extremely significant p<0.01.
[0107] The results are shown in Table 2. Compared with the blank control group, the TC, TG and LDL-C contents in the serum of the modeling group rats increased significantly, and the HDL-C content decreased significantly (p<0.05). Compared with the modeling group, the TC, TG and LDL-C contents in the serum of the rats fed with the nutritional composition containing dietary fiber of Example 1 and Example 4 decreased significantly (p<0.05), while the HDL-C content increased (p<0.05). The TC, TG and LDL-C contents in the serum of the rats fed with the composition of Comparative Examples 1-8 decreased to varying degrees, but the degree of decrease was not as good as that of the rats fed with the nutritional composition of Example 1 and Example 4, and the HDL-C content increased slightly, but the degree of increase was not as good as that of the rats fed with the nutritional composition of Example 1 and Example 4. It can be seen from the comparison of Example 1 with Comparative Examples 1-3 that the dietary fiber, probiotics, red ginseng extract, phytosterols and L-carnitine in the composition coordinately enhance the regulation of blood lipid content. It can be seen from Comparative Examples 5-8 that the dietary fiber composed of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber has a better effect of regulating blood lipids. It can be seen that the nutritional composition containing dietary fiber provided by the present invention has a more significant ability to regulate the blood lipid level of rats.
[0108] Test Example 2
[0109] 1.1 Experimental design and methods
[0110] 70 healthy female volunteers aged 30-45 years, weighing 63-70kg, with a body fat percentage of 30-32%, who are not pregnant or lactating, were selected as research subjects, without contraindications or other physical diseases. Exclusion criteria: Psychiatric patients with serious diseases of the heart, liver, kidney and hematopoietic system; patients with malignant tumors and immune diseases; patients with other primary intestinal diseases; those who take items related to the test function in a short period of time, which may affect the judgment of the results; patients who cannot cooperate to complete this experiment.
[0111] 70 volunteers were randomly divided into a control group and an observation group, a total of 7 groups, 10 people in each group: the volunteers of the observation groups AH consumed the nutritional compositions containing dietary fiber of Examples 1, 2, 3, 5 and the compositions of Comparative Examples 1-2 in the morning and evening, 25 g / meal each time, and had three normal meals.
[0112] After 30 days, the volunteers' weight and body fat percentage were calculated and measured with a body fat scale before getting up and eating. After getting up for 30 days without eating, venous blood was drawn to measure triglyceride TG and total cholesterol TC levels.
[0113] 1.2 Test indicators
[0114] Body weight, body fat percentage, triglyceride TG and total cholesterol TC levels were measured.
[0115] 1.3 Experimental Results
[0116] 1.3.1 Weight and body fat percentage
[0117] Table 3 Weight and body fat percentage test levels
[0118]
[0119] As shown in Table 3, after the volunteers in the AD group consumed the nutritional compositions containing dietary fiber of Examples 1, 2, 3, and 5, their weight decreased by 2.89-3.38 kg and their body fat percentage decreased by 2.06-2.78% after 30 days; the volunteers in the E and F groups consumed the compositions of Comparative Examples 1 and 2, and their weight decreased by 1.48 kg and 1.51 kg, respectively, and their body fat percentage decreased by 0.71% and 1.02%, respectively, after 30 days; while the weight and body fat percentage of the volunteers in the control group increased. It can be seen that the dietary fiber provided by the present invention has a more obvious weight loss effect.
[0120] 1.3.2 Blood lipid levels
[0121] Dyslipidemia is one of the common metabolic markers in obese patients, and blood lipid levels can reflect the systemic lipid metabolism status.
[0122] Table 4 TG and TC levels in blood
[0123]
[0124] * indicates that compared with Example 1, the difference is significant p<0.05, and the difference is extremely significant p<0.01.
[0125] As shown in Table 4, the AD group is the test results of the levels of triglyceride TG and total cholesterol TC in the volunteers after 30 days after consuming the nutritional compositions containing dietary fiber of Examples 1, 2, 3, and 5, and the EF group is the test results of the levels of triglyceride TG and total cholesterol TC in the volunteers after 30 days after taking the compositions of Comparative Examples 1-2. It can be seen that the TG and TC in the AD group decreased significantly, which is better than the improvement degree of the E group and the F group, and there is a significant difference. It can be seen that the nutritional composition containing dietary fiber of the present invention can regulate the blood lipid level of the human body.
[0126] In summary, the dietary fiber composition provided by the present invention can regulate blood lipid levels, improve metabolic capacity, inhibit the increase of body weight and fat, and has a good weight loss effect. At the same time, the dietary fiber composition of the present invention is rich in multiple nutrients, supplements multiple nutritional elements of the human body, enhances immunity, and can be eaten as food or health care products.
[0127] Although the embodiments of the present description have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present description, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A composition containing dietary fiber, characterized in that The invention comprises the following components in parts by weight: 55-85 parts of dietary fiber, 0.1-7 parts of probiotics, 1-9 parts of red ginseng extract, 0.01-2 parts of phytosterols, 0.01-0.2 parts of L-carnitine, 0.05-0.8 parts of multivitamins and 0.05-0.1 parts of mineral elements; The dietary fiber is a mixture of laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber, and the mass ratio of the laver fiber, oat fiber, strawberry fiber, chickpea fiber and black rice fiber is (1-3):(1-2):(0.5-1):(0.5-1):(0.5-1); the probiotics are a mixture of Bifidobacterium longum, Lactobacillus rhamnosus and Lactobacillus acidophilus, and the mass ratio of the Bifidobacterium longum, Lactobacillus rhamnosus and Lactobacillus acidophilus is (5-20):(3-15):(1-5), and the viable bacteria concentration of the probiotics is 6×10 8 CFU / g-1×10 11 CFU / g; The method for extracting laver fiber comprises the following steps: S1, washing the laver, stewing it at a high pressure of 0.2-0.8MPa for 1-3min, cooling, filtering and crushing it; S2, dispersing the crushed laver crumbs with 5-10 times of water, adding 0.1-1.0‰ of protease, enzymolyzing it in a water bath at 25-37℃ for 6-24h, centrifuging, and immersing the centrifugal filter residue in ethanol water for 2-3 times, each time for 5-10min, wherein the mass ratio of ethanol to water is (1-2): (1-2 ) to obtain laver residue; S3, disperse S2 laver residue with 5-10 times water, add 0.1-1.0‰ cellulase, enzymolyze in a water bath of 25-37°C for 12-24h, filter, freeze-dry, and grind into powder to obtain the laver fiber; the complex vitamin contains vitamin C, vitamin E and pantothenic acid; the mass ratio of vitamin C, vitamin E and pantothenic acid is (1-2):(1-2):(1-2); the mineral elements include one or more of iron, zinc, calcium, magnesium and selenium.
2. The dietary fiber-containing composition according to claim 1, characterized in that The composition also comprises 25-40 parts of whey protein, 10-20 parts of fish maw oligopeptide and 20-40 parts of soy protein.
3. The method for preparing the composition containing dietary fiber according to any one of claims 1 to 2, characterized in that: The method comprises the following steps: weighing various raw materials according to the formula, and mixing them evenly to obtain the product.
4. A nutritional preparation, characterized in that The invention comprises the dietary fiber-containing composition according to any one of claims 1 to 2 and auxiliary materials.
5. Use of the dietary fiber-containing composition according to any one of claims 1 to 2 in the preparation of fat-reducing foods or health products.
Citation Information
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