Kit for the determination of immunoglobulin G by latex immunoturbidimetry

Abstract

The invention discloses a kit for latex immunoturbidimetric determination of urine immunoglobulin G, and relates to the field of biological detection technology. The kit includes R1 reagent and R2 reagent; the R1 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 1-10g / L bovine serum albumin, 10-50g / L reaction enhancer, 0.1-10mL / L preservative, 100-300mM NaCl, 2-6g / L surfactant; the R2 reagent includes 10-200mM tris(hydroxymethyl)aminomethane, 100-300mM NaCl, 1-5g / L antibody-coupled latex particles, 1-5g / L glycerol, 5-10g / L methyl-β-cyclodextrin, 1-10g / L bovine serum albumin, 2-6g / L surfactant and 0.1-10mL / L preservative. The kit of the present invention has good repeatability and stability.

Description

Technical Field

[0001] The invention relates to the technical field of biological detection, in particular to a kit for determining urine immunoglobulin G by latex immunoturbidimetry. Background Art

[0002] Immunoglobulin is a kind of globulin in the human body. It has the activity and structure of antibodies. It is a type of protein produced by lymphocytes under the stimulation of antigens. It has immune and physiological regulatory effects. Immunoglobulin mainly exists in plasma and is also commonly found in other body fluids and tissues. Immunoglobulin is divided into five categories, namely IgG, IgA, IgD, IgM and IgE. They have similar basic structures and are composed of two identical heavy chains (H chains) with a relative molecular mass of about 60,000-80,000 and two identical light chains (H chains) with a relative molecular mass of about 20,000-30,000. They have a certain symmetry, and the peptide chains are connected by disulfide bonds to form a tetrapeptide chain structure.

[0003] Immunoglobulin G (IgG), the molecular weight of IgG is about 150kDa, and it is composed of four polypeptide chains, including two identical heavy chains and two identical light chains, which are connected by disulfide bonds. IgG accounts for about 70-80% of the total immunoglobulin. IgG can be divided into four subclasses: IgG1, IgG2, IgG3, and IgG4. The classification is based on the different antigenicity of the main chain of the molecule and the location and number of disulfide bonds. IgG molecules can be divided into Fab fragments and Fc fragments. The Fab fragment includes the antigen binding site, and the amino acid sequence of the Fc fragment is basically unchanged, which is called the constant region. Normal urine contains very small amounts of protein, which is filtered out by the normal glomerular capillary wall and most of it is reabsorbed by the glomerulus. The most notable feature of IgG is that it can specifically recognize antigens. Antigens can induce activated B lymphocytes to produce IgG antibodies, and IgG can specifically bind to them to form antigen-antibody complexes. When the glomerulus is diseased, immunoglobulin G may be present in the urine due to increased permeability or damage and rupture of the glomerular membrane.

[0004] Chinese patent CN113156136A discloses a kit for detecting immunoglobulin G in urine, the kit comprising reagent R1 and reagent R2; the reagent R1 comprises the following components: Tris base, polyethylene glycol, polyvinyl pyrrolidone, sodium chloride, surfactant S9, EDTA-Na 2and sodium benzoate; the reagent R2 comprises the following components: tris base, sheep anti-human immunoglobulin G polyclonal antibody, animal serum, sodium benzoate, trehalose, EDTANa2 and Tween. The invented kit has high sensitivity, wide linear range and good repeatability, and can accurately detect low-value samples; at the same time, the kit of the application also has good stability, the reagent can be stably stored for 14 months, the filtration step before the use of the reagent is omitted, the operation steps are simplified and the accuracy of the test results is improved.

[0005] Chinese patent CN105929171A discloses a high-performance human urine immunoglobulin G detection kit, which includes reagent 2, which is a latex particle solution labeled with rabbit anti-human immunoglobulin G polyclonal antibody; wherein the latex particle size ranges from 130nm to 300nm, and the amount of antibody in each liter of reagent 2 is 10ml to 20ml. This invention can replace imported reagents, save patient expenses, and improve detection efficiency.

[0006] Currently, there is still a lack of a detection kit for immunoglobulin G in urine based on latex immunoturbidimetry with high detection accuracy and stability. Summary of the invention

[0007] The invention aims to provide an immunoglobulin G detection kit based on latex immunoturbidimetry, the kit has high stability and accuracy, and can be used for the detection of immunoglobulin G in urine.

[0008] To achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows:

[0009] In one aspect, the present invention provides a detection kit for immunoglobulin G, the kit comprising reagent R1 and reagent R2;

[0010] The R1 reagent includes 10-200 mM tris(hydroxymethyl)aminomethane, 1-10 g / L bovine serum albumin, 10-50 g / L reaction enhancer, 0.1-10 mL / L preservative, 100-300 mM NaCl, and 2-6 g / L surfactant;

[0011] The R2 reagent includes 10-200 mM tris(hydroxymethyl)aminomethane, 100-300 mM NaCl, 1-5 g / L antibody-coupled latex particles, 1-5 g / L glycerol, 5-10 g / L methyl-β-cyclodextrin, 1-10 g / L bovine serum albumin, 2-6 g / L surfactant and 0.1-10 mL / L preservative.

[0012] Preferably, the R1 reagent comprises 100-200 mM tris(hydroxymethyl)aminomethane, 2-8 g / L bovine serum albumin, 10-40 g / L reaction enhancer, 1-5 mL / L preservative, 100-200 mM NaCl, and 4-6 g / L surfactant;

[0013] The R2 reagent includes 100-200 mM tris(hydroxymethyl)aminomethane, 100-200 mM NaCl, 1-4 g / L antibody-coupled latex particles, 1-3 g / L glycerol, 5-8 g / L methyl-β-cyclodextrin, 2-8 g / L bovine serum albumin, 4-6 g / L surfactant and 1-5 mL / L preservative.

[0014] Preferably, the reaction enhancer is selected from one or more of PEG2000, PEG4000, PEG6000, PEG8000, PEG12000, and PEG20000; further, the reaction enhancer is PEG4000.

[0015] Preferably, the antibody is a monoclonal antibody or a polyclonal antibody.

[0016] Furthermore, the antibody is goat anti-human immunoglobulin polyclonal antibody, rabbit anti-human immunoglobulin polyclonal antibody, mouse anti-human immunoglobulin polyclonal antibody.

[0017] Furthermore, the antibody is a sheep anti-human immunoglobulin polyclonal antibody.

[0018] Preferably, the latex particles are polystyrene latex particles with a particle size of 200-500 nm;

[0019] According to some embodiments of the present invention, the surface of the latex particles is modified with functional groups;

[0020] Furthermore, the functional group is selected from carboxyl, amino, hydroxyl, aldehyde, hydrazide or chloromethyl.

[0021] Furthermore, the latex particles are carboxylated polystyrene latex particles.

[0022] Preferably, the mass ratio of the antibody to the latex particles is 1:(10-30).

[0023] Furthermore, the antibody-coupled latex particles are goat anti-human immunoglobulin G antibody latex particles.

[0024] Preferably, the preservative is selected from one or more of sodium azide, phenol, Proclin 300, p-hydroxybenzoic acid, 2-chloroacetamide, sodium dehydroacetate, sodium methyl Nepal gold ester, sodium p-hydroxyglycinate, ethyl 4-hydroxybenzoate, 5-bromo-5-nitro-1,3-dioxane, and methylisothiazolinone.

[0025] More preferably, the preservative is Proclin300.

[0026] Preferably, the surfactant is selected from one or more of Tween 20, Tween 80, Triton 100, polyoxyethyl lauryl ether, alkylphenol polyoxyethylene ether, and sodium lauryl sulfate.

[0027] Furthermore, the surfactant is a combination of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate.

[0028] Furthermore, the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is (1-3): (0.1-1): (1-5).

[0029] Furthermore, the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is (2-3): (0.5-1): (2-4).

[0030] Most preferably, the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:3.

[0031] Preferably, the kit further comprises standard substances and / or quality control substances.

[0032] Furthermore, the standard substance includes immunoglobulin G, bovine serum albumin and preservatives at different concentrations.

[0033] Furthermore, the quality control product includes immunoglobulin G, bovine serum albumin and a preservative.

[0034] Preferably, the pH of the reagent R1 and the reagent R2 is 6.0-8.0.

[0035] Preferably, the dominant wavelength of detection is 570 nm.

[0036] Preferably, the method using the immunoglobulin G detection kit comprises the following steps:

[0037] (1) The absorbance change value of the calibration substance is △A (A 校准 -A 空白 ) / min as the ordinate, and the corresponding concentration C calibration as the abscissa to draw a calibration curve;

[0038] (2) According to the sample's △A(A 测定 -A 空白 ) / min and find the corresponding sample concentration on the calibration curve.

[0039] In another aspect, the present invention provides use of the immunoglobulin G detection kit in detecting immunoglobulin G in urine.

[0040] According to some embodiments of the present invention, the method for detecting immunoglobulin G comprises the following steps:

[0041] (1) Draw a standard curve: Take the absorbance change value of the calibrator △A(A 校准 -A 空白 ) / min is the ordinate, and the corresponding concentration C 校准 Plot the calibration curve for the abscissa;

[0042] (2) Mix reagent R1 and the sample to be tested, incubate at 37°C for 5 min, add reagent R2, and immediately read the absorbance value A1. Read the absorbance value A2 after 5 min, and calculate the absorbance change △A=A2-A1;

[0043] (3) According to the sample's △A(A 测定 -A 空白 ) / min and find the corresponding sample concentration on the calibration curve.

[0044] The detection principle of the kit of the present invention is that the immunoglobulin G in the sample combines with the sheep anti-human immunoglobulin G antibody latex particles to form an antigen-antibody complex, which produces a certain turbidity. The turbidity level is proportional to the antigen content when a certain antibody is present. The content of immunoglobulin G in the sample can be calculated by measuring the turbidity change (absorbance change) at a specific wavelength.

[0045] The beneficial effects of the present invention are:

[0046] The invention provides a detection kit for immunoglobulin G based on latex immunoturbidimetry. The kit has strong stability, good detection effect after long-term storage, high detection accuracy and good repeatability, can be used for the detection of immunoglobulin G in urine, and has broad application prospects. DETAILED DESCRIPTION

[0047] In order to make the technical means, creative features, purpose and efficacy of the present invention easy to understand, the present invention is further explained in conjunction with specific embodiments below, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. In the following embodiments, unless otherwise specified, the operating methods used are conventional operating methods, the equipment used are conventional equipment, and the equipment materials used in each embodiment are the same.

[0048] Example 1 Preparation of Immunoglobulin G Detection Kit

[0049] 1. Preparation of sheep anti-human immunoglobulin G antibody latex particles

[0050] (1) adding polystyrene latex microspheres with a particle size of 250 nm into MES buffer (pH 6.5, purchased from Shanghai Kanglang Biotechnology Co., Ltd.) for dilution to obtain a solution with a mass concentration of 2% of polystyrene latex microspheres;

[0051] (2) Dissolve goat anti-human immunoglobulin G antibody in MES buffer (pH 6.5) to a final concentration of 1 mg / ml;

[0052] (3) Add EDAC (0.5 mg / ml) to the polystyrene latex microsphere solution, mix well, activate and react at 45°C for 30 min, wash away EDAC with MES buffer to obtain activated polystyrene latex microspheres, add goat anti-human immunoglobulin G antibody solution (mass ratio of antibody to latex particles is 1:20), mix well, incubate at 37°C for 2 h, add blocking solution (2% Tween 20, 1% BSA and MES buffer pH 6.5) and react at room temperature for 3 h;

[0053] (4) Centrifuge at 4°C (14,000 rpm, 40 min), wash 3-4 times with MES buffer to remove unbound antibodies, and obtain goat anti-human immunoglobulin G antibody latex particles.

[0054] 2. Preparation of R1 and R2 reagents

[0055] R1 reagent: 100mM tris(hydroxymethyl)aminomethane, 5g / L bovine serum albumin, 20g / L PEG4000, 1mL / L Proclin300, 150mM NaCl, 5.5g / L surfactant;

[0056] R2 reagent: 100 mM tris(hydroxymethyl)aminomethane, 150 mM NaCl, 3 g / L goat anti-human immunoglobulin G antibody latex particles, 2 g / L glycerol, 6 g / L methyl-β-cyclodextrin, 5 g / L bovine serum albumin, 5.5 g / L surfactant and 1 mL / L Proclin300.

[0057] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:3.

[0058] Example 2

[0059] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0060] 2. Preparation of R1 and R2 reagents

[0061] R1 reagent: 100mM tris(hydroxymethyl)aminomethane, 5g / L bovine serum albumin, 20g / L PEG4000, 1mL / L Proclin300, 150mM NaCl, 4.9g / L surfactant;

[0062] R2 reagent: 150 mM tris(hydroxymethyl)aminomethane, 200 mM NaCl, 4 g / L goat anti-human immunoglobulin G antibody latex particles, 3 g / L glycerol, 8 g / L methyl-β-cyclodextrin, 6 g / L bovine serum albumin, 4.9 g / L surfactant and 2 mL / L Proclin300.

[0063] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:1:4.

[0064] Example 3

[0065] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0066] 2. Preparation of R1 and R2 reagents

[0067] R1 reagent: 150mM tris(hydroxymethyl)aminomethane, 8g / L bovine serum albumin, 40g / L PEG4000, 5mL / L Proclin300, 200mM NaCl, 5.5g / L surfactant;

[0068] R2 reagent: 150 mM tris(hydroxymethyl)aminomethane, 200 mM NaCl, 4 g / L goat anti-human immunoglobulin G antibody latex particles, 4 g / L glycerol, 8 g / L methyl-β-cyclodextrin, 8 g / L bovine serum albumin, 5.5 g / L surfactant and 5 mL / L Proclin300.

[0069] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:3.

[0070] Example 4

[0071] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0072] 2. Preparation of R1 and R2 reagents

[0073] R1 reagent: 200mM tris(hydroxymethyl)aminomethane, 10g / L bovine serum albumin, 40g / L PEG4000, 10mL / L Proclin300, 300mM NaCl, 6g / L surfactant;

[0074] R2 reagent: 200 mM tris(hydroxymethyl)aminomethane, 200 mM NaCl, 5 g / L goat anti-human immunoglobulin G antibody latex particles, 5 g / L glycerol, 10 g / L methyl-β-cyclodextrin, 10 g / L bovine serum albumin, 6 g / L surfactant and 10 mL / L Proclin300.

[0075] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 3:1:2.

[0076] Comparative Example 1

[0077] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0078] 2. Preparation of R1 and R2 reagents

[0079] The preparation of R1 reagent is the same as in Example 1.

[0080] Preparation of R2 reagent:

[0081] R2 reagent: 100 mM tris(hydroxymethyl)aminomethane, 150 mM NaCl, 3 g / L goat anti-human immunoglobulin G antibody latex particles, 2 g / L sucrose, 6 g / L sorbitol, 5 g / L bovine serum albumin, 5.5 g / L surfactant and 1 mL / L Proclin300.

[0082] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:3.

[0083] Comparative Example 2

[0084] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0085] 2. Preparation of R1 and R2 reagents

[0086] The preparation of R1 reagent is the same as that of Example 1. The difference between R2 reagent and Example 1 is only that the surfactant is different, and the surfactant of Comparative Example 2 is Tween 20, Span 80 and Triton 100; the mass ratio of Tween 20, Span 80 and Triton 100 is 2:0.5:3.

[0087] Comparative Example 3

[0088] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0089] 2. Preparation of R1 and R2 reagents

[0090] The preparation of R1 reagent is the same as in Example 1.

[0091] The difference between the R2 reagent and Example 1 is only that the mass ratio of the surfactants is different. The surfactants in Comparative Example 3 are Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 4:1:6.

[0092] Comparative Example 4

[0093] 1. The preparation of sheep anti-human immunoglobulin G antibody latex particles is the same as in Example 1.

[0094] 2. Preparation of R1 and R2 reagents

[0095] The preparation of R1 reagent is the same as in Example 1.

[0096] R2 reagent: 210 mM tris(hydroxymethyl)aminomethane, 310 mM NaCl, 6 g / L goat anti-human immunoglobulin G antibody latex particles, 6 g / L glycerol, 12 g / L methyl-β-cyclodextrin, 10 g / L bovine serum albumin, 6.6 g / L surfactant and 12 mL / L Proclin300.

[0097] The surfactant is Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; the mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:3.

[0098] Experimental Example 1 Determination of Immunoglobulin G

[0099] Testing instruments: Applicable to Hitachi 7600 / 7180 / 3500 / 008AS, Mindray BS2000, Dirui CS600 / CS2000, Abbott C8000 / C16000, Toshiba TBA-2000FR / TBA-FX8, Beckman AU680 / AU5800, Siemens ADVIA 2400 / XPT / CH930 / DimensionEXL, Roche C501 / C701 / C702. The testing instrument used in this experiment is Hitachi 7600.

[0100] 1. Test samples

[0101] (1) Urine samples can be stored at 2-8°C for 3 days. If the urine is turbid, it should be centrifuged before testing and the supernatant should be collected.

[0102] (2) Bilirubin ≤250 mg / L, ascorbic acid ≤400 mg / L, and creatinine ≤5 g / L in the sample will not significantly interfere with the test results. Confirmation is required when the limits are exceeded.

[0103] 2. Detection method

[0104] (1) Basic parameters

[0105] 1) Reaction type: endpoint method;

[0106] 2) Temperature: 37°C;

[0107] 3) Dominant wavelength: 570nm;

[0108] 4) Sub-wavelength: / ;

[0109] 5) Sample volume: 2 μl;

[0110] 6) Reagent R1 volume: 150 μl;

[0111] 7) Reagent R2 volume: 50 μl;

[0112] 8) Reaction direction: ascending reaction.

[0113] (2) Determination

[0114] 1) Mix reagent R1 and the sample to be tested;

[0115] 2) Incubate the mixed solution at 37°C for 5 min;

[0116] 3) Add reagent R2, read the absorbance value A1 immediately, read the absorbance value A2 after 5 minutes, and calculate the absorbance change △A=A2-A1.

[0117] (3) Calibration:

[0118] 1) It is recommended to use the matching calibrator for calibration. The calibrator is liquid and can be used directly. The amount of calibrator should be consistent with the sample amount.

[0119] 2) Calibration method: This product adopts multi-point calibration.

[0120] 3) Calibration frequency: after reagent batch number is changed; according to the needs of measurement results.

[0121] 4. Quality Control

[0122] 1) The quality control product is a liquid and can be used directly. The amount of quality control product used is the same as the sample amount.

[0123] 2) Use control substances (quality control products) to monitor the operating procedures. If the quality control results are under control, it is considered that the operating procedures are correct and the results are accurate and reliable; if the quality control results are out of the allowable range, corrective measures should be taken.

[0124] 5. Calculation

[0125] The absorbance change of the calibration material is △A(A 校准 -A 空白 ) / min is the ordinate, and its corresponding concentration C 校准 Draw a calibration curve as the horizontal axis. 测定 -A 空白 ) / min and find the corresponding sample concentration on the calibration curve.

[0126] 6. Performance testing of the kit

[0127] (1) Linear range

[0128] Experimental steps:

[0129] (1) The immunoglobulin G standard (200 mg / L) was diluted with normal saline. The theoretical concentrations after dilution were 200 mg / L, 100 mg / L, 50 mg / L, 25 mg / L, 12.5 mg / L, 6.25 mg / L, 3.125 mg / L and 0 mg / L. Each concentration was measured 3 times and the average value (y i ), with dilution concentration (x i ) is the independent variable, with y i The linear regression equation was obtained for the dependent variable. The correlation coefficient r was calculated according to the following formula, and the results are shown in Table 1 below.

[0130]

[0131] Where: r is the correlation coefficient, x i is the dilution ratio, y i is the mean value of the results of each sample, is the mean dilution ratio, is the total mean of the sample measurement results.

[0132] Experimental results:

[0133] Table 1.

[0134]

[0135]

[0136] It can be seen from the above table that the linear correlation coefficients of the test kits prepared in Examples 1-4 are all greater than 0.999, while the linear correlation coefficients of the test kits prepared in Comparative Examples 1-4 are all lower than 0.990.

[0137] (2) Repeatability

[0138] Detection method: The urine sample was tested using the detection kit of Examples 1-4 (the sample contained 20 mg / L immunoglobulin G), repeated 5 times, and the test results are shown in the following table. CV = SD / M × 100%, SD is the standard deviation of the 5 test results, and Mean is the average of the 5 test results.

[0139] Table 2.

[0140]

[0141] It can be seen from the above table that the detection kits prepared in Examples 1-4 of the present invention have good repeatability, and their coefficient of variation CV values ​​are all lower than 2%. The CV value of the detection kit prepared in Example 1 is lower than 1%, while the coefficient of variation of the detection kits prepared in Comparative Examples 1-4 is greater than 5%, which is poorer in repeatability than that of Examples 1-4.

[0142] (3) Stability

[0143] Under storage conditions of 37°C, the prepared test kit was used to test the quality control products (the concentrations of immunoglobulin G were 150 mg / L, 100 mg / L and 50 mg / L, respectively) in October, March, June and September. Each sample was tested 5 times and the average value was taken. The test results are shown in the following table.

[0144] Table 3.

[0145]

[0146] Table 4.

[0147]

[0148] Table 5.

[0149]

[0150]

[0151] Table 6.

[0152]

[0153] It can be seen from the above table that the relative deviations of the detection kits prepared in Examples 1-4 of the present invention from the labeled values ​​were less than 5% when stored for 9 months, indicating that the kits of the present invention have good long-term stability.

[0154] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A detection kit for immunoglobulin G, characterized in that: The kit comprises R1 reagent and R2 reagent; The R1 reagent includes 10-200 mM tris(hydroxymethyl)aminomethane, 1-10 g / L bovine serum albumin, 10-50 g / L reaction enhancer, 0.1-10 mL / L preservative, 100-300 mM NaCl, and 2-6 g / L surfactant; The R2 reagent includes 10-200 mM tris(hydroxymethyl)aminomethane, 100-300 mM NaCl, 1-5 g / L antibody-coupled latex particles, 1-5 g / L glycerol, 5-10 g / L methyl-β-cyclodextrin, 1-10 g / L bovine serum albumin, 2-6 g / L surfactant and 0.1-10 mL / L preservative; The surfactant is a combination of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate; The mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is (2-3): (0.5-1): (2-4).

2. The detection kit according to claim 1, characterized in that The reaction enhancer is selected from one or more of PEG2000, PEG4000, PEG6000, PEG8000, PEG12000 and PEG20000.

3. The detection kit according to claim 1, characterized in that The preservative is selected from one or more of sodium azide, phenol, Proclin 300, p-hydroxybenzoic acid, 2-chloroacetamide, sodium dehydroacetate, sodium methyl Nepal gold ester, sodium p-hydroxyglycinate, ethyl 4-hydroxybenzoate, 5-bromo-5-nitro-1,3-dioxane, and methylisothiazolinone.

4. The detection kit according to claim 1, characterized in that The mass ratio of Triton 100, polyoxyethyl lauryl ether and sodium lauryl sulfate is 2:0.5:

3.

5. The detection kit according to claim 1, characterized in that The kit also includes standard products and / or quality control products.

6. Use of the detection kit according to any one of claims 1 to 5 in the preparation of a product for detecting immunoglobulin G in urine.

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