Pre-packaged blister, detection assembly and detection device

Abstract

The application relates to a pre-packaged liquid capsule, a detection assembly and a detection device, which comprise a capsule body with a hollow interior to form a sealed containing cavity, at least one weak part formed on the wall surface of the capsule body, and reagents sealed in the containing cavity; when the pre-packaged liquid capsule is extruded by external force, the weak part can be broken to release the reagents in the containing cavity. The pre-packaged liquid capsule, the detection assembly and the detection device provided by the application can greatly solve the problem of complex manufacturing process of the pre-packaged liquid capsule and the breaking part or the easy-tearing part, and effectively improve the problem of excessively large volume of the micro-fluidic chip and the POCT detection device.

Description

Technical Field

[0001] This application relates to the field of microfluidic chip reagent pre-packaging technology, and in particular to a pre-packaged liquid capsule, a detection component, and a detection device. Background Technology

[0002] Microfluidic chips are microanalytical systems that integrate sample pretreatment, mixing, reaction, separation, and detection into one or more chips. They are increasingly used in disease detection, food safety, environmental monitoring, and drug screening. Early microfluidic chips involved manually adding reagents to the chip, a method that was complex and prone to error. In recent years, reagent pre-encapsulation technology has been gradually applied to microfluidic chips.

[0003] However, current reagent pre-encapsulation technologies are generally complex in structure and require microfluidic chips with sufficiently large cavities to accommodate the pre-encapsulated liquid sacs. When there are many pre-encapsulated liquid sacs and the required filling volume is large, the microfluidic chip needs to be enlarged or thickened to meet the testing requirements, which affects the miniaturization design of the chip and leads to excessively large point-of-care testing (POCT) devices. Summary of the Invention

[0004] Therefore, it is necessary to provide a pre-packaged liquid capsule, a detection component, and a detection device to address the issue of excessive volume.

[0005] A first aspect of this application provides a pre-packaged liquid capsule, comprising: a capsule body having an interior hollow to form a sealed containment cavity, wherein at least one weak portion is formed on the wall surface of the capsule body; and a reagent sealed in the containment cavity; wherein when the pre-packaged liquid capsule is subjected to external pressure, the weak portion can rupture to release the reagent in the containment cavity.

[0006] In one embodiment, the weak portion is a scorching groove formed by laser ablation; or, the weak portion is a tool scratch; or, the weak portion is a shaped groove.

[0007] In one embodiment, the weak part is shaped as a circle, rhombus, straight line, cross, or radiating star.

[0008] In one embodiment, the depth of the weak portion is S, and the wall thickness of the capsule is M, satisfying: 0.1*M≤S≤0.9*M.

[0009] In one embodiment, the external force is between 0N and 20N, and when the pre-packaged liquid bladder is subjected to the external force, the weak portion can remain in its original state; and / or, the external force is greater than 20N, and when the pre-packaged liquid bladder is subjected to the external force, the weak portion can rupture to release the reagent in the containment cavity.

[0010] In one embodiment, the capsule is made of one of plastic, aluminum foil, or aluminum-plastic composite material.

[0011] In one embodiment, the capsule includes a first housing and a second housing, the first housing and the second housing overlapping each other to define the receiving cavity;

[0012] The weak point is located in the second housing; the side of the first housing opposite to the receiving cavity is used to withstand external pressure.

[0013] In one embodiment, the second housing has a connection end for connecting to the outside, the connection end being located on the side of the second housing away from the receiving cavity and next to the weak part.

[0014] In one embodiment, the first shell is hemispherical, cylindrical, or square; and / or the second shell is flat or arc-shaped.

[0015] A second aspect of this application provides a detection component, including a microfluidic chip and the aforementioned pre-encapsulated liquid bladder, wherein the pre-encapsulated liquid bladder is fixed on the microfluidic chip;

[0016] When the pre-packaged liquid capsule is squeezed by an external force, the weak part can break open to release the reagent in the containment cavity, and the reagent enters the microfluidic chip.

[0017] In one embodiment, the microfluidic chip has a sample inlet, a mixing chamber, and a connecting groove. The connecting groove connects the sample inlet and the mixing chamber, and forms a liquid inlet in the sample inlet. The pre-encapsulated liquid bladder is fixed to the sample inlet, and the weak part is located above the liquid inlet.

[0018] A third aspect of this application provides a detection apparatus, including the detection components described above.

[0019] The beneficial effects are:

[0020] The pre-packaged liquid capsule, detection component, and detection device of this application embodiment, by setting a capsule and reagent, the capsule is hollow to form a sealed containment cavity, and at least one weak point is formed on the wall of the capsule; the reagent is sealed in the containment cavity; when the pre-packaged liquid capsule is squeezed by external force, the weak point can break to release the reagent in the containment cavity; thus, there is no need to set a separate puncture part to puncture the pre-packaged liquid capsule, but instead the reagent is released from the weak point by breaking at a fixed point, which not only simplifies the microfluidic chip structure, but also makes the microfluidic chip structure design more flexible, and further improves the chip detection performance; moreover, the weak point is simple to manufacture and is very easy to realize in terms of process, and the size of the rupture opening and the force required for the weak point to break can be flexibly adjusted according to the actual application requirements; furthermore, the pre-packaged liquid capsule adheres to the microfluidic chip, and the microfluidic chip does not need to provide a large cavity when the weak point breaks to release the liquid, which is conducive to the miniaturization design of the microfluidic chip, and thus effectively reduces the size of the POCT device. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of a pre-packaged liquid capsule provided in some embodiments of this application.

[0022] Figure 2 This is a schematic diagram of the structure of a pre-packaged liquid capsule provided in some embodiments of this application.

[0023] Figure 3 The diagram shows the internal structure of a pre-packaged liquid bladder provided in some embodiments of this application. In the diagram, the arrows indicate the direction of the applied external force.

[0024] Figure 4 This is a schematic diagram of the internal structure of a pre-packaged liquid capsule provided in some embodiments of this application, wherein a weak part is broken to release the reagent in the containment cavity. In the figure, the arrows indicate the direction of the breakage of the weak part.

[0025] Figure 5 This is a schematic diagram of the structure of the detection component provided in some embodiments of this application.

[0026] Figure 6 This is a schematic diagram illustrating the interaction between a microfluidic chip and a pre-packaged liquid capsule provided in some embodiments of this application. Detailed Implementation

[0027] The embodiments of the technical solution of this application will now be described in detail with reference to the accompanying drawings. These embodiments are only used to more clearly illustrate the technical solution of this application and are therefore merely examples, and should not be used to limit the scope of protection of this application.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms “comprising” and “having”, and any variations thereof, in the specification, claims, and foregoing description of the drawings are intended to cover non-exclusive inclusion.

[0029] In the description of the embodiments of this application, if the technical terms such as "first" and "second" appear, these terms are used only for descriptive purposes to distinguish different objects, and should not be construed as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features.

[0030] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0031] In the description of the embodiments in this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, and B existing alone. Additionally, the character " / " in this document generally indicates that the preceding and following related objects have an "or" relationship.

[0032] In the description of the embodiments of this application, if the term "multiple" appears, "multiple" means at least two (including two), such as two, three, etc., unless otherwise explicitly specified. Similarly, if the term "multiple sets" appears, "multiple sets" refers to two or more sets (including two sets), and if the term "multiple pieces" appears, "multiple pieces" refers to two or more pieces (including two pieces).

[0033] In the description of the embodiments of this application, if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0034] In the description of the embodiments of this application, unless otherwise explicitly specified and limited, the technical terms "installation," "connection," "joining," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this application according to the specific circumstances.

[0035] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0036] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0037] The first aspect of this application provides a pre-packaged liquid capsule 100 for use in a microfluidic chip.

[0038] See Figures 1 to 4 As shown, the pre-packaged liquid capsule 100 includes a capsule body 10 and a reagent 20. The capsule body 10 is hollow to form a sealed receiving cavity 11, and at least one weak point 12 is formed on the wall surface of the capsule body 10. The capsule body 10 can be made of a homogeneous material; in some embodiments, the capsule body 10 can be made of plastic material for storing sample components or auxiliary components used for immediate testing and analysis; in other embodiments, the capsule body 10 can also be made of aluminum foil or aluminum-plastic composite material. This application does not limit the scope of the embodiments in this study.

[0039] Reagent 20 is sealed in the receiving cavity 11. Reagent 20 can be an organic solution or an inorganic solution. The organic solution can be one or more of alcohol solvents, hydrocarbon solvents, ester solvents, and ether solvents, and the inorganic solution can be one or more of pure water, acidic solvents, and alkaline solvents. The volume of reagent 20 is from 10 μL to 2000 μL; the amount and composition of reagent 20 can be specifically set according to different test items, and this application embodiment does not limit this. A quantitative amount of reagent 20 can be introduced into the microfluidic chip 200 (mentioned below) for corresponding detection when needed, which is convenient and fast, avoids manually adding the reaction reagent to the microfluidic chip 200, simplifies the operation method, and has small error.

[0040] When the pre-packaged liquid sac 100 is squeezed by external force, the weak part 12 can break open to release the reagent 20 in the containment cavity 11; thus, there is no need to set a separate puncture part to puncture the pre-packaged liquid sac 100, which greatly solves the problem of complex manufacturing process of the pre-packaged liquid sac 100 and the fracture or easily torn part, and enables the detection component using microfluidic chip 200 to be further miniaturized, effectively reducing the size of POCT device.

[0041] Furthermore, by using the method of releasing reagent 20 from the weak point 12, the structure of the microfluidic chip 200 is not only simplified, but the structural design of the microfluidic chip 200 is also made more flexible, further improving the chip's detection performance. Moreover, the weak point is simple to fabricate and very easy to implement in terms of process. The size of the rupture opening and the force required for the weak point to rupture can be flexibly adjusted according to actual application requirements. In addition, the pre-encapsulated liquid capsule adheres to the microfluidic chip, and the microfluidic chip does not need to provide a large cavity when the weak point ruptures to release the liquid, which is conducive to the miniaturization design of the microfluidic chip 200 and thus effectively reduces the size of the POCT device.

[0042] The weak part 12 can be formed in various ways.

[0043] In some embodiments, the weak portion 12 is a scorching groove formed by laser ablation. Specifically, a laser marking machine can be used to ablate the wall surface of the capsule 10 to a certain extent, thereby forming a scorching groove that is weaker in strength. In other embodiments, the weak portion 12 is a tool scratch, that is, the surface of the capsule 10 can be damaged to a certain extent by cutting with a knife, thereby forming a tool scratch area that is weaker in strength. Furthermore, in other embodiments, the weak portion 12 is a molding groove. That is, the surface of the capsule 10 can be damaged to a certain extent by using a mold or other structural means, thereby forming a tool scratch area that is weaker in strength. The embodiments of this application do not limit the formation method of the weak portion 12.

[0044] Optionally, the shape of the weak point 12 can be one of the following: circular, rhomboid, straight, cross-shaped, or radial star-shaped; the specific shape can be adjusted and selected according to the depth it withstands during fracture.

[0045] In some possible embodiments, see Figures 1 to 4 As shown, the depth of the weak part 12 is S, and the wall thickness of the capsule 10 is M, satisfying: 0.1*M≤S≤0.9*M. This ensures that the strength of the weak part 12 is lower than that of the walls of other areas of the capsule 10. When subjected to a certain external force F, all parts of the receiving cavity 11 experience equal compressive force. When the external force F reaches a certain limit value, the weak part 12 can rupture before other structures of the capsule 10, thereby releasing the reagent 20 from the receiving cavity 11.

[0046] Taking a wall thickness M of 50 μm for the capsule 10 as an example, the depth S of the weak part 12 can be 15 μm, 18 μm, 20 μm, 24 μm, 26 μm, 30 μm, 32 μm, 35 μm, 38 μm, or 40 μm. Preferably, the depth S of the weak part 12 can be 35 ± 5 μm.

[0047] It should be noted that the limit value of this external force F is usually related to the material, thickness and shape of the weak part 12. Through design, the limit value of this external force F can be made lower than the value of the compression force exerted on the bladder 10 by the POCT device. In other words, if the POCT device applies a force of 30N-100N by pressing the compression block against the capsule 10, it is necessary to ensure that when the pre-packaged liquid capsule 100 is subjected to a pressure of 30N-100N, the weak part 12 ruptures to release the liquid. This strength requirement can be achieved by setting the material, thickness, and shape of the weak part 12 on the capsule 10 so that the weak part 12 can withstand an external force F of 20N. Thus, when the external force F is between 0N and 20N, the pre-packaged liquid capsule 100 withstands the external force compression, the weak part 12 can remain in its original shape, and the capsule 10 maintains the seal on the reagent 20. However, when the external force is greater than 20N, the pre-packaged liquid capsule 100 withstands the external force compression, and the weak part 12 can rupture to release the reagent 20 in the containment cavity 11.

[0048] Optionally, the groove depth S and groove width of the weak part 12 can be scanned using a measuring instrument to ensure that the weak part 12 meets the design values ​​and prevent errors.

[0049] In some possible embodiments, see Figures 1 to 4 As shown, the capsule 10 includes a first shell 13 and a second shell 14, which overlap each other to define a receiving cavity 11.

[0050] The first shell 13 can be a hollow structure with one open end, and the second shell 14 can be a plate-like structure. The first shell 13 covers the open side of the second shell 14 so that the first shell 13 and the second shell 14 together define a receiving cavity 11 for containing the reagent 20. Alternatively, both the first shell 13 and the second shell 14 can be hollow structures with one open side, with the open side of the first shell 13 covering the open side of the second shell 14. Of course, the capsule 10 formed by the first shell 13 and the second shell 14 can be of various shapes, such as a simple three-dimensional structure like a cuboid or cylinder, a hemisphere, or a sphere, or a complex three-dimensional structure composed of simple three-dimensional structures like cuboids, cylinders, hemispheres, or spheres. The embodiments of this application are not limited in this respect.

[0051] A weak point 12 is provided on the second housing 14; the side of the first housing 13 facing away from the receiving cavity 11 is used to withstand external pressure. When the first housing 13 is squeezed by an external force F, it is transmitted to the receiving cavity 11, and the reagent 20 provides equal squeezing force to all walls of the capsule 10. The weak point 12 on the second housing 14 can break open to release the reagent 20 in the receiving cavity 11. In this way, it can be ensured that the area of ​​the capsule 10 being squeezed does not coincide with the area where the liquid is released, and that the reagent 20 can flow completely into the microfluidic chip 200 (mentioned below) for corresponding detection, which is convenient and fast, and avoids reagent 20 residue in the squeezed area.

[0052] In some possible embodiments, see Figures 1 to 4 As shown, the first shell 13 can be designed as a hemispherical shell, cylindrical shell, or square shell with an opening on one side; the second shell 14 can be designed as a flat plate or an arc-shaped plate. The first shell 13 covers the opening side of the second shell 14 so that the first shell 13 and the second shell 14 together define a receiving cavity 11 for containing the reagent 20. In addition, the second shell 14 can also be hemispherical, cylindrical, or square shell, depending on the design, and this application does not limit it in this regard.

[0053] The first housing 13 and the second housing 14 can typically be made of the same material, i.e., aluminum foil, aluminum-plastic composite, or plastic. The first housing 13 and the second housing 14 can be connected and sealed at the joint using a heat-sealing process to ensure that the reagent 20 is sealed within the receiving cavity 11 without leakage. Alternatively, the first housing 13 and the second housing 14 can also be made of other different materials and sealed together using processes such as bonding, laser processing, heat sealing, or ultrasonic welding.

[0054] In some possible embodiments, see Figure 3 and Figure 4 As shown, the direction of the external force F is from the outside to the inside of the cavity 11, and the direction of the fracture of the weak part 12 is from the cavity 11 to the outside.

[0055] Specifically, in combination Figure 3 and Figure 4 When the pre-packaged liquid capsule 100 is squeezed by an external force F pointing into the receiving cavity 11, the external force F is transmitted inward to the reagent 20 in the receiving cavity 11. The reagent 20 provides equal squeezing force to all the walls of the capsule 10. The weak part 12 on the second shell 14 can be cracked from the receiving cavity 11 in the direction pointing outward, that is, cracked from the inside out to release the reagent 20 in the receiving cavity 11.

[0056] In some possible embodiments, see Figures 1 to 4 As shown, a connection end 15 for connection with the outside is formed on the second housing 14. The connection end 15 is located on the side of the second housing 14 away from the receiving cavity 11 and next to the weak part 12. The second housing 14 can be glued to the external sample inlet 210 (mentioned below) by double-sided adhesive or mechanical fixation.

[0057] The connecting end 15 can be a ring-shaped rigid structure surrounding the weak part 12; the connecting end 15 can also be multiple connecting blocks distributed circumferentially along the second shell 14, subject to the design.

[0058] The weak point 12 can usually be located at the geometric center of the second housing 14; it can also be located to the side of the geometric center of the second housing 14, depending on the design.

[0059] A second aspect of this application provides a detection component.

[0060] See Figures 1 to 6 As shown, the detection component includes a microfluidic chip 200 and the aforementioned pre-encapsulated liquid sac 100, with the pre-encapsulated liquid sac 100 fixed to the microfluidic chip 200. Specifically, the pre-encapsulated liquid sac 100 can be fixed to the microfluidic chip 200 by adhesive bonding. When the pre-encapsulated liquid sac 100 is subjected to external pressure, the weak part 12 can rupture to release the reagent 20 in the receiving cavity 11. The reagent 20 enters the microfluidic chip 200, thereby performing the corresponding detection. This method is convenient and quick, avoiding the need to manually add the reaction reagent to the microfluidic chip 200, simplifying the operation method and reducing errors.

[0061] Optionally, a pre-packaged liquid capsule 100 can be assembled on a microfluidic chip 200.

[0062] Optionally, multiple pre-packaged liquid sacs 100 can be assembled on a microfluidic chip 200, and the multiple pre-packaged liquid sacs 100 can be arranged circumferentially on the microfluidic chip 200.

[0063] In some possible embodiments, the microfluidic chip 200 has a sample inlet 210, a mixing chamber 220, and a connecting groove 230. The connecting groove 230 connects the sample inlet 210 and the mixing chamber 220 respectively, and the connecting groove 230 forms a liquid inlet 231 in the bottom of the sample inlet 210. The connecting end 15 of the pre-encapsulated liquid bladder 100 can be fixed to the sample inlet 210 by means of double-sided adhesive, and the weak part 12 is located above the liquid inlet 231.

[0064] Thus, the pre-packaged liquid sac 100 can be pressed using the pressing head on the detection device (mentioned below). When the first housing 13 is squeezed by external force, the weak part 12 on the second housing 14 can be broken to release the reagent 20 in the receiving cavity 11. The reagent 20 enters the sample inlet 210 of the microfluidic chip 200 from the receiving cavity 11, and enters the mixing cavity 220 from the sample inlet 210 through the connecting groove 230. Under the action of centrifugal force, the released reagent 20 enters the reaction cavity (not shown) and participates in the reaction of the reagent tray (not shown) to mix with other reagents or samples, thereby performing the corresponding detection. It is convenient, fast and has small detection error.

[0065] A third aspect of this application provides a detection apparatus, which includes the detection components described above.

[0066] In addition, the detection device may include a pressing head (not shown) that can press the pre-encapsulated liquid sac 100 on the detection component to release the reagent 20 for detection. Those skilled in the art will understand that the detection device also includes components that may be present to complete the detection. For example, centrifuges, heaters, etc., which will not be listed here.

[0067] The detection device in this application embodiment can be a POCT detection device.

[0068] Specifically, the assembly and testing process is as follows:

[0069] S10. The reagent 20 is pre-packaged in the capsule 10. The volume of the pre-packaged reagent 20 is 1 mL. The material used for the capsule 10 is either aluminum foil or aluminum-plastic composite material.

[0070] S20. A weak portion 12 is formed on the second housing 14 of the pre-encapsulated liquid bladder 100. The depth of the weak portion 12 is 30±5 μm.

[0071] S30. The pre-packaged liquid capsule 100 and the microfluidic chip 200 are aligned and assembled using a positioning fixture. Double-sided adhesive can be used to bond the connecting end 15 to fix the pre-packaged liquid capsule 100 and the microfluidic chip 200.

[0072] S40. Place the assembled microfluidic chip in the detection device and press it for 4 seconds. The pressing value can be 50 N. Release reagent 20 into the mixing chamber 220.

[0073] S50, the detection device generates a centrifugal speed of 2700 rpm, causing the released reagent 20 to enter the equal volume structure for separation. The equal volume of reagent 20 enters the reaction chamber to participate in the reaction, thereby completing the detection.

[0074] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0075] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A pre-packaged liquid capsule characterized in that, The pre-packaged liquid capsule is used in a micro-fluidic chip, and comprises: a capsule body (10) which is hollow inside to form a sealed containing cavity (11), and at least one weak part (12) is formed on the wall of the capsule body (10); and a reagent (20) which is sealed in the containing cavity (11); when the pre-packaged liquid capsule is extruded by an external force, the weak part (12) can be broken to release the reagent (20) in the containing cavity (11); the capsule body (10) comprises a first shell (13) and a second shell (14), the first shell (13) and the second shell (14) are mutually covered to define the containing cavity (11); the weak part (12) is arranged on the second shell (14), and the side of the first shell (13) which is away from the containing cavity (11) is used to bear the extrusion of the external force; a connecting end (15) is formed on the second shell (14) for external connection, the connecting end (15) is located on the side of the second shell (14) which is away from the containing cavity (11) and is beside the weak part (12).

2. The pre-packaged liquid capsule of claim 1, wherein, the weak part (12) is a laser-burned groove formed by laser burning; or, the weak part (12) is a cutter scratch; or, the weak part (12) is a shaped groove.

3. The pre-packaged liquid capsule of claim 1, wherein, the shape of the weak part (12) is one of a circle, a diamond, a straight line, a cross or a radial star.

4. The pre-packaged liquid capsule of claim 1, wherein, the depth of the weak part (12) is S, the wall thickness of the capsule body (10) is M, and 0.1*M≤S≤0.9*M is satisfied.

5. The pre-packaged liquid capsule of any one of claims 1 to 4, wherein, the external force is 0N-20N, when the pre-packaged liquid capsule is extruded by the external force, the weak part (12) can remain original; and / or, the external force is greater than 20N, when the pre-packaged liquid capsule is extruded by the external force, the weak part (12) can be broken to release the reagent (20) in the containing cavity (11).

6. The pre-packaged liquid capsule of any one of claims 1 to 4, wherein, the capsule body (10) is made of one of plastic, aluminum foil or aluminum-plastic composite material.

7. The pre-packaged liquid capsule of claim 1, wherein, the first shell (13) is in a shape of a semi-spherical shell, a cylindrical shell or a square shell; and / or, the second shell (14) is in a shape of a flat plate or an arc-shaped plate.

8. A detection assembly characterized by, a micro-fluidic chip (200) and the pre-packaged liquid capsule (100) according to any one of claims 1 to 7 are comprised, and the pre-packaged liquid capsule (100) is fixed on the micro-fluidic chip (200); when the pre-packaged liquid capsule (100) is extruded by an external force, the weak part (12) can be broken to release the reagent (20) in the containing cavity (11), and the reagent (20) enters the micro-fluidic chip (200).

9. The detection assembly of claim 8, wherein, a sample inlet groove (210), a mixing cavity (220) and a connecting groove (230) are formed on the micro-fluidic chip (200), the connecting groove (230) connects the sample inlet groove (210) and the mixing cavity (220) respectively, the connecting groove (230) forms a liquid inlet (231) in the sample inlet groove (210), the pre-packaged liquid capsule (100) is fixed on the sample inlet groove (210), and the weak part (12) is located above the liquid inlet (231).

10. A detection device, characterized in that The detection assembly as claimed in claim 8 or 9.

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