A cell sorting device

Abstract

The present invention provides a cell sorting device, including a frame, a sorting component and a plurality of clamping mechanisms. The sorting component includes a sorting column and a liquid holding bottle arranged up and down. The open end of the liquid holding bottle can be sealed and connected to the liquid outlet end of the sorting column. The clamping mechanism has a magnetic field, and the clamping mechanism is arranged on the frame. The clamping mechanism is used to clamp the sorting component, and can be kept on the frame or rotated in a vertical plane. The present invention clamps the sorting component by the clamping mechanism to rotate, so that the solution moves back and forth between the sorting column and the liquid holding bottle in the sorting component, so that the target cells in the solution pass through the iron beads in the sorting column for multiple times, thereby increasing the contact between the target cells and the iron beads, thereby increasing the amount of target cells at the sorting location and improving the sorting efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of biological equipment, and in particular to a cell sorting device. Background Art

[0002] Cell sorting mainly refers to a technique that uses the characteristics of cells to separate a specific cell subpopulation from a mixed cell sample. Cell sorting is usually divided into two categories: one is density gradient centrifugation based on the physical properties of cells, i.e. flow cytometry; the other is based on immune recognition characteristics, including fluorescence-activated cell sorting and magnetic-activated cell sorting, i.e. magnetic bead sorting.

[0003] Among them, magnetic bead sorting is based on the target cell surface antigen binding to magnetic beads, which are adsorbed and retained in the magnetic field when passing through the magnetic field, thereby achieving the separation of target cells. Magnetic bead sorting is widely used because of its simple operation and low cost. Miltenyi magnetic bead sorter is a more common magnetic bead sorter on the market. It has a bracket with multiple working positions on the bracket. The working position of the sorter can fix the sorting column, which is filled with iron beads. There are permanent magnets on the working positions. The magnetic field of the permanent magnet is amplified by the iron beads in the sorting column. The target cell surface antigen in the solution combines with the magnetic beads and passes through the iron beads and is retained on the iron beads by the magnetic field.

[0004] However, the flow path of the solution in the sorting column is relatively short. When the cells in the solution pass through the iron beads in the sorting column, the target cells close to the iron beads are easily magnetically attracted to the iron beads through the magnetic beads on them, while the target cells far away from the outer wall of the iron beads are easily lost along with the solution and cannot be fully magnetically attracted by the iron beads, resulting in a reduction in the number of sorting and a decrease in sorting efficiency. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a cell sorting device, which can solve or at least alleviate one or more of the above-mentioned problems and other problems in the prior art.

[0006] The present invention provides a cell sorting device, comprising:

[0007] frame;

[0008] The sorting assembly includes a sorting column and a liquid holding bottle arranged vertically; and

[0009] A plurality of clamping mechanisms, having magnetic fields, are arranged on the frame and used to clamp the sorting assembly, and can be kept on the frame and can also be rotated in a vertical plane;

[0010] The sorting column has a liquid inlet end and a liquid outlet end. The sorting column is filled with iron beads. The liquid inlet end of the sorting column can be closed. One end of the liquid receiving bottle is open, and the open end of the liquid receiving bottle can be sealed and connected to the liquid outlet end of the sorting column.

[0011] Preferably, it also includes:

[0012] A motor, arranged on the frame;

[0013] A connecting member, rotatably disposed on the frame and connected to the output shaft of the motor; and

[0014] A plurality of drive rings, coaxial with the output shaft of the motor and connected to the connecting member;

[0015] The clamping mechanism corresponds to the driving ring one by one; the clamping mechanism can be kept on the frame and not move; the clamping mechanism can be driven by the corresponding driving ring to rotate.

[0016] Preferably, the frame comprises a plurality of pairs of mounting rings sleeved outside the driving ring; the plurality of pairs of mounting rings correspond to the plurality of driving rings one by one; the two mounting rings in each pair of mounting rings are symmetrically arranged; an annular first slide groove is provided on the opposite side of each pair of mounting rings; a plurality of evenly distributed first holes are provided on the side wall of the driving ring;

[0017] The clamping mechanism comprises:

[0018] a first connecting block, located between each pair of the mounting rings;

[0019] Two arc-shaped sliding blocks are respectively connected to two ends of the first connecting block and slidably matched with the first sliding groove on the same side;

[0020] a connecting rod, slidably disposed on the first connecting block and slidably inserted into the first hole on the corresponding driving ring; and

[0021] The clamping component is used for clamping the sorting component.

[0022] Preferably, a second annular slide groove is provided on the opposite side of each pair of mounting rings; the second slide groove is located outside the first slide groove; the second slide groove includes:

[0023] Arc segment;

[0024] an inclined section having a deeper end and a shallower end, wherein the shallower end is connected to one end of the arc section, the depth of the shallower end is greater than the depth of the arc section, and the distance between the deeper end and the axis of the mounting ring is greater than the distance between the shallower end and the axis of the mounting ring; and

[0025] a horizontal section extending in the horizontal direction, having a deeper end and a shallower end, wherein the deeper end is connected to the deeper end of the inclined section, and the depth of the deeper end is greater than the depth of the deeper end of the inclined section, and the shallower end is connected to the other end of the arc section, and the depth of the shallower end is less than the depth of the arc section;

[0026] The clamping mechanism also includes:

[0027] a second connecting block, slidably disposed on the first connecting block, movable along the radial direction of the driving ring, and capable of driving the connecting rod to move; and

[0028] Two limiting rods are slidably arranged on both sides of the second connecting block, and ends of the two limiting rods can elastically abut against the second sliding groove on the same side.

[0029] Preferably, a second hole is formed on a surface of the first connection block facing away from the driving ring; a coaxial and penetrating third hole is formed at the bottom of the second hole; a fourth hole is formed on a surface of the second connection block facing the first connection block; one end of the connecting rod is located in the fourth hole, and the other end of the connecting rod passes through the second hole and the third hole and then faces the driving ring;

[0030] The clamping mechanism also includes:

[0031] a limit block connected to one end of the connecting rod, located in the fourth hole, and slidably matched with the fourth hole along the axial direction;

[0032] a first spring, sleeved outside the connecting rod and located in the second hole, with one end of the first spring abutting against the bottom of the second hole and the other end of the first spring abutting against the limit block; and

[0033] A second spring is located in the fourth hole, one end of the second spring abuts against the limit block, and the other end of the second spring abuts against the bottom of the fourth hole;

[0034] The first spring and the second spring are respectively located on two sides of the limiting block.

[0035] Preferably, the clamping assembly includes a clamping block; the clamping block is connected to the end of the second connecting block facing away from the first connecting block; the clamping block is provided with a first funnel hole and a second funnel hole symmetrically arranged up and down; the first funnel hole and the second funnel hole are connected by a connecting hole; the first funnel hole is used to place a sorting column; the second funnel hole is used to place a liquid receiving bottle.

[0036] Preferably, the clamping assembly further comprises two clamping members arranged symmetrically with each other; both of the two clamping members are connected to the first connecting block; and the ends of the two clamping members away from the first connecting block can respectively abut against the liquid inlet end of the sorting column and the closed end of the liquid receiving bottle.

[0037] Preferably, third sliding grooves are provided on the side surfaces of the second connecting block corresponding to the two clamping members; and the clamping members are slidably matched with the third sliding grooves on the same side.

[0038] Preferably, fifth holes are respectively opened on the side surfaces of the second connecting block facing the two mounting rings; the opening of the fifth hole is provided with a limiting ring that contracts inwardly; one end of the limiting rod abuts against the second sliding groove on the same side, and the other end of the limiting rod passes through the limiting ring and is connected with a stopper; the stopper abuts against the limiting ring; a third spring is provided in the fifth hole; one end of the third spring abuts against the stopper, and the other end of the third spring abuts against the bottom of the fifth hole.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] In the technology of the present invention, the sorting component is clamped by a clamping mechanism and rotated, so that the solution moves back and forth between the sorting column and the liquid holding bottle in the sorting component, so that the target cells in the solution pass through the iron beads in the sorting column multiple times, thereby increasing the contact between the target cells and the iron beads, thereby increasing the amount of target cells at the sorting point and improving the sorting efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0042] Figure 1 is a three-dimensional diagram of a cell sorting device in one embodiment of the present invention;

[0043] Figure 2 for Figure 1 A three-dimensional diagram of the sorting assembly;

[0044] Figure 3 for Figure 1 Another stereogram of (retaining only a pair of mounting rings and a clamping mechanism);

[0045] Figure 4 for Figure 3 Another stereogram of (retaining only one mounting ring);

[0046] Figure 5 for Figure 4 A perspective view of the middle mounting ring;

[0047] Figure 6 for Figure 5 An enlarged schematic diagram of point C;

[0048] Figure 7 for Figure 4 A three-dimensional diagram of the coordination between the clamping mechanism and the sorting assembly;

[0049] Figure 8 for Figure 7 BB surface cross-sectional view;

[0050] Fig. 9 for Figure 3 AA surface view;

[0051] Fig.10 for Fig. 9 Enlarged schematic diagram of point D.

[0052] Reference numerals:

[0053] 10. frame; 11. mounting ring; 12. first slide groove; 13. second slide groove; 131. arc segment; 132. inclined segment; 133. horizontal segment;

[0054] 20. Sorting assembly; 21. Sorting column; 211. Liquid inlet end; 212. Liquid outlet end; 22. Liquid receiving bottle; 221. Open end;

[0055] 30. Clamping mechanism; 31. First connecting block; 311. Second hole; 312. Third hole; 32. Arc-shaped slider; 33. Connecting rod; 34. Clamping assembly; 341. Clamping block; 342. First funnel hole; 343. Second funnel hole; 344. Connecting hole; 345. Clamping member; 35. Second connecting block; 351. Fourth hole; 352. Third slide groove; 353. Fifth hole; 354. Limiting ring; 355. Stopper; 356. Third spring; 36. Limiting rod; 37. Limiting block; 38. First spring; 39. Second spring;

[0056] 40. Motor; 41. Connector; 42. Drive ring; 421. First hole. DETAILED DESCRIPTION

[0057] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0058] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0059] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0060] In addition, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, the meaning of "plurality" is more than two, unless otherwise clearly and specifically defined.

[0061] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0063] See also Figures 1 to 10 This embodiment provides a cell sorting device, including a frame 10, a sorting component 20 and a plurality of clamping mechanisms 30.

[0064] The sorting assembly 20 includes a sorting column 21 and a liquid receiving bottle 22 arranged in a vertical manner. The sorting column 21 has a liquid inlet end 211 and a liquid outlet end 212, with reference to the structure of the sorting column 21 in the prior art. The sorting column 21 is filled with iron beads. The liquid inlet end 211 of the sorting column 21 can be closed, and the liquid inlet end 211 of the sorting column 21 can be closed by screwing a cover. One end of the liquid receiving bottle 22 is open (the other end is a closed end), and the open end 221 of the liquid receiving bottle 22 can be sealed and connected to the liquid outlet end 212 of the sorting column 21. Specifically, the appearance of the liquid receiving bottle 22 is substantially the same as that of the sorting column 21, except that there is no iron bead in the liquid receiving bottle 22, and the bottle mouth of the liquid receiving bottle 22 can be sleeved on the liquid outlet end 212 of the sorting column 21. The bottle mouth of the liquid receiving bottle 22 can be made of rubber material, so that the bottle mouth of the liquid receiving bottle 22 is connected to the liquid outlet end 212 of the sorting column 21 and forms a seal therewith.

[0065] The clamping mechanism 30 has a magnetic field. The clamping mechanism 30 is arranged on the frame 10. The clamping mechanism 30 is used to clamp the sorting component 20. It can be kept on the frame 10 or rotated in the vertical plane. Specifically, the vertical plane is perpendicular to the horizontal plane. The center lines of the sorting column 21 and the liquid holding bottle 22 are collinear, and the center line is recorded as S1. When the clamping mechanism 30 clamps the sorting component 20 and rotates, the trajectory is circular, and the tangent line of the position where the clamping mechanism 30 is located when rotating is recorded as S2, and S1 and S2 are parallel to each other.

[0066] In this embodiment, Figure 3When the clamping mechanism 30 remains stationary on the frame 10, the sorting assembly 20 can be fixedly mounted on the clamping mechanism 30, and the solution is injected through the liquid inlet end 211 of the sorting column 21, and then the liquid inlet end 211 of the sorting column 21 is closed. Then the clamping mechanism 30 rotates counterclockwise on the frame 10, driving the sorting assembly 20 to rotate. During the counterclockwise rotation of the sorting assembly 20 by 360 degrees, the upper and lower positions of the sorting column 21 and the liquid bottle 22 in the sorting assembly 20 change, so that the solution flows from the sorting column 21 into the liquid bottle 22, and then flows back from the liquid bottle 22 into the sorting column 21, and then flows from the sorting column 21 into the liquid bottle 22 again. The solution passes through the iron beads in the sorting column 21 many times. Under the magnetic field of the clamping mechanism 30, the iron beads magnetically attract the target cells in the solution many times, which can increase the number of target cells sorted and improve the sorting efficiency. In addition, the iron beads are filled into the sorting column 21, and the solution flows through the gaps between the iron beads. The target cells close to the outer wall of the iron beads are easily magnetically attracted, while the target cells wrapped in the middle of the solution are not easily magnetically attracted by the iron beads. During the 360-degree rotation of the sorting component 20, not only the upper and lower positions of the sorting column 21 and the liquid holding bottle 22 are changed many times, but also the sorting column 21 is tilted many times, and the tilt angle changes all the time. This causes the solution to change its flow direction when flowing through the iron beads in the sorting column 21, and the target cells in the solution are fully in contact with the iron beads, so that more target cells can be magnetically attracted by the iron beads, further increasing the amount of sorted target cells.

[0067] Then, after the clamping mechanism 30 remains stationary, the liquid holding bottle 22 is removed from the sorting column 21, and a new liquid holding bottle 22 is connected. Then, the sorting component 20 is removed from the clamping mechanism 30, the liquid inlet end 211 of the sorting column 21 is opened, and buffer is added to elute the target cells into the new liquid holding bottle 22.

[0068] In one embodiment, the cell sorting device further includes a motor 40 , a connector 41 and a plurality of drive rings 42 .

[0069] The motor 40 is arranged on the frame 10. Specifically, the motor 40 is fixed on the frame 10 by bolt connection or welding. The connecting member 41 is fixedly connected to the output shaft of the motor 40, and the connecting member 41 is rotatably arranged on the frame 10. Specifically, both ends of the connecting member 41 are connected to the frame 10 through bearings, and a plurality of driving rings 42 are fixedly connected to the connecting member 41 at the same time. The driving ring 42 is coaxial with the output shaft of the motor 40, and the plurality of driving rings 42 are located in the middle of the connecting member 41. The clamping mechanism 30 corresponds to the driving ring 42 one by one. The clamping mechanism 30 can be kept on the frame 10 and not move. The clamping mechanism 30 can be driven by the corresponding driving ring 42 to rotate.

[0070] In this embodiment, the clamping mechanism 30 and the driving ring 42 are disengageable. When the clamping mechanism 30 and the driving ring 42 are connected, the driving ring 42 can drive the clamping mechanism 30 to rotate around the axis of the driving ring 42, thereby driving the sorting assembly 20 to rotate.

[0071] In one embodiment, the frame 10 includes a plurality of pairs of mounting rings 11 sleeved outside the driving ring 42. The plurality of pairs of mounting rings 11 correspond to the plurality of driving rings 42 one by one. The two mounting rings 11 in each pair of mounting rings 11 are symmetrically arranged. An annular first slide groove 12 is provided on the opposite side of each pair of mounting rings 11. A plurality of evenly distributed first holes 421 are provided on the side wall of the driving ring 42, and the axial direction of the first holes 421 is the same as the radial direction of the driving ring 42.

[0072] The clamping mechanism 30 includes a first connecting block 31 , two arc-shaped sliding blocks 32 , a connecting rod 33 and a clamping assembly 34 .

[0073] The first connection block 31 is located between each pair of mounting rings 11. Two arc-shaped sliders 32 are respectively connected to the two ends of the first connection block 31, and the arc-shaped slider 32 is slidably matched with the first slide groove 12 on the same side. The connecting rod 33 is slidably arranged on the first connection block 31, and the connecting rod 33 can be slidably inserted into the first hole 421 on the corresponding driving ring 42. The clamping assembly 34 is used to clamp the sorting assembly 20.

[0074] In this embodiment, the clamping mechanism 30 can remain stationary on the mounting ring 11. When the clamping mechanism 30 needs to be rotated, the connecting rod 33 is inserted into the first hole 421 on the driving ring 42. The rotation of the driving ring 42 drives the first connecting block 31 to rotate, thereby driving the entire clamping mechanism 30 to rotate.

[0075] In one embodiment, an annular second slide groove 13 is formed on the opposite side of each pair of mounting rings 11 . The second slide groove 13 is located outside the first slide groove 12 .

[0076] The second sliding groove 13 includes an arc segment 131 , an inclined segment 132 and a horizontal segment 133 .

[0077] The arc segment 131 is connected to the inclined segment 132 , one end of the inclined segment 132 away from the arc segment 131 is connected to the horizontal segment 133 , and one end of the horizontal segment 133 away from the inclined segment 132 is connected to the arc segment 131 . The three are annular as a whole and together constitute the second slide groove 13 .

[0078] The inclined section 132 has a deeper end and a shallower end, wherein the shallower end is connected to one end of the arc section 131 and the depth of the shallower end is greater than the depth of the arc section 131 , and the distance between the deeper end and the axis of the mounting ring 11 is greater than the distance between the shallower end and the axis of the mounting ring 11 .

[0079] The horizontal section 133 extends in the horizontal direction, and at the same time extends in the radial direction of the drive ring 42. The horizontal section 133 has a deeper end and a shallower end, wherein the deeper end is connected to the deeper end of the inclined section 132, and the depth of the deeper end is greater than the depth of the deeper end of the inclined section 132, and the shallower end is connected to the other end of the arc section 131, and the depth of the shallower end is less than the depth of the arc section 131.

[0080] The clamping mechanism 30 further includes a second connecting block 35 and two limiting rods 36 .

[0081] The second connection block 35 is slidably disposed on the first connection block 31. The second connection block 35 can move along the radial direction of the drive ring 42. The second connection block 35 can drive the connection rod 33 to move. Specifically, the second connection block 35 can be fixedly connected to the connection rod 33 to drive the connection rod 33 to move, or can indirectly drive the connection rod 33 to move through other transmission methods. Two limit rods 36 are slidably disposed on both sides of the second connection block 35, and the ends of the limit rods 36 can elastically abut against the second slide groove 13 on the same side. Specifically, the limit rod 36 can be elastically retractable, and its end abuts against the second slide groove 13.

[0082] In this embodiment, the second connecting block 35 drives the connecting rod 33 to move and then inserts into the first hole 421 on the driving ring 42, so that when the driving ring 42 rotates, the first connecting ring can be driven to rotate along the first sliding groove 12 through the connecting rod 33. At the same time, when the second connecting block 35 drives the connecting rod 33 to move, the limiting rods 36 on both sides of the second connecting block 35 are moved from the deeper end ( Figure 6 The right end of the horizontal section 133 passes through the shallow end ( Figure 6 When the driving ring 42 rotates, the first connecting block 31 rotates accordingly. Since the second connecting block 35 is slidably connected to the first connecting block, the second connecting block 35 also rotates along with the first connecting block 31. The ends of the limiting rods 36 on both sides of the second connecting block 35 slide in the circular arc segment 131.

[0083] Until the second connecting block 35 rotates nearly 360 degrees, the ends of the limiting rods 36 on both sides of the second connecting block 35 slide into the deeper end of the inclined section 132 ( Figure 6), the limiting rod 36 drives the second connecting block 35 away from the driving ring 42, so that the second connecting block 35 drives the connecting rod 33 to be gradually pulled out from the first hole 421 on the driving ring 42, and then when the end of the limiting rod 36 passes through the shallower end of the inclined section 132 and slides into the deeper end of the horizontal section 133, the connecting rod 33 is completely pulled out from the first hole 421. Even if the driving ring 42 continues to rotate, the connecting rod 33 and the driving ring 42 have been separated at this time, and the second connecting block 35 is stopped in place by the limiting rod 36. At this time, the entire clamping mechanism 30 is in a horizontal state, and the sorting component 20 is in a vertical state. The operator can operate the sorting component 20, and the clamping mechanism 30 remains in place, realizing the parking of the clamping mechanism. Due to the setting of the inclined section 132, the clamping mechanism 30 can be separated from the driving ring 42 by itself, that is, after rotating one circle, the clamping mechanism 30 can be parked at the position where the horizontal section 133 is located by itself. The other clamping mechanisms 30 that have not rotated a full circle can still operate. When the operator operates the current clamping mechanism 30, other clamping mechanisms 30 are not affected, thereby improving efficiency.

[0084] In one embodiment, a second hole 311 is formed on a surface of the first connection block 31 facing away from the drive ring 42. A coaxial and penetrating third hole 312 is formed at the bottom of the second hole 311. A fourth hole 351 is formed on a surface of the second connection block 35 facing the first connection block 31. One end of the connection rod 33 is located in the fourth hole 351, and the other end of the connection rod 33 passes through the second hole 311 and the third hole 312 and then faces the drive ring 42.

[0085] The clamping mechanism 30 further includes a limit block 37 , a first spring 38 and a second spring 39 .

[0086] The stopper 37 is connected to one end of the connecting rod 33. The stopper 37 is located in the fourth hole 351 and slidably cooperates with the fourth hole 351 along the axial direction. The first spring 38 is sleeved outside the connecting rod 33. The first spring 38 is located in the second hole 311. One end of the first spring 38 abuts against the bottom of the second hole 311, and the other end of the first spring 38 abuts against the stopper 37. The second spring 39 is located in the fourth hole 351. One end of the second spring 39 abuts against the stopper 37, and the other end of the second spring 39 abuts against the bottom of the fourth hole 351. The first spring 38 and the second spring 39 are respectively located on both sides of the stopper 37.

[0087] In this embodiment, the second connection block 35 can move close to the first connection block 31, and in the process of approaching, the second spring 39 compresses and drives the limit block 37 to move toward the driving ring 42, so that the limit block 37 compresses the first spring 38, and the limit block 37 moves to drive the connecting rod 33 to elastically abut against the outer wall of the driving ring 42, and the limit rods 36 on both sides of the second connection block 35 enter the arc segment 131 from the deeper end of the horizontal segment 133 through its shallower end. Since the depth of the arc segment 131 is greater than the shallower end of the horizontal segment 133, the end of the limit rod 36 cannot retreat to the deeper end of the horizontal segment 133, so that the second connection block 35 has a driving force on the limit block 37 through the second spring 39, and then the limit block 37 drives the connecting rod 33 to abut against the outer wall of the driving ring 42. The driving ring 42 is in a state of uniform rotation. When the driving ring 42 rotates to the right position and the first hole 421 approaches the connecting rod 33, the connecting rod 33 can be inserted into the first hole 421 by itself. When the operator is operating, he only needs to place the sorting component 20 on the clamping component 34, push the second connecting block 35 into place, and then the connecting rod 33 will be automatically inserted into the first hole 421 and can be driven by the driving ring 42, and then through the cooperation of the limiting rod 36 on the second connecting block 35 and the inclined section 132, the second connecting block 35 will automatically move away from the driving ring 42, and the connecting rod 33 will automatically disengage from the driving ring 42. During the whole process, you only need to push the second connecting block 35 below, which is more convenient.

[0088] In one embodiment, the clamping assembly 34 includes a clamping block 341. The clamping block 341 is connected to an end of the second connecting block 35 away from the first connecting block 31. The clamping block 341 is provided with a first funnel hole 342 and a second funnel hole 343 symmetrically arranged up and down. The first funnel hole 342 and the second funnel hole 343 are connected by setting a connecting hole 344. The first funnel hole 342 is used to place the sorting column 21. The second funnel hole 343 is used to place the liquid bottle 22. Specifically, the shape of the sorting column 21 matches the first funnel hole 342, and the liquid bottle 22 is arranged in the same manner as the second funnel hole 343. The size of the liquid outlet end 212 of the sorting column 21 is smaller than its liquid inlet end 211, and the size of the open end 221 of the liquid receiving bottle 22 is smaller than its closed end. When the liquid outlet end 212 of the sorting column 21 passes through the connecting hole 344 and is connected to the open end 221 of the liquid receiving bottle 22, there is a certain connection strength between the sorting column 21 and the liquid receiving bottle 22, and they will not be easily separated. The connecting block drives the sorting assembly 20 to rotate during the rotation process. In addition, the clamping block 341 is internally provided with a permanent magnet to provide a magnetic field.

[0089] In one embodiment, the clamping assembly 34 further includes two symmetrically arranged clamping members 345. Both clamping members 345 are connected to the first connection block 31. The ends of the two clamping members 345 away from the first connection block 31 can respectively abut against the liquid inlet end 211 of the separation column 21 and the closed end of the liquid receiving bottle 22.

[0090] After the sorting assembly 20 is installed on the clamping block 341, in the process of pushing the clamping block 341 to drive the second connecting block 35 close to the first connecting block 31, the liquid inlet end 211 of the sorting column 21 moves to the inner side of one clamping piece 345 and abuts against it, and the closed end of the liquid bottle 22 moves to the inner side of another clamping piece 345 and abuts against it. Thus, the sorting assembly 20 will not be separated during the rotation process. When the rotation is completed, when the second connecting block 35 moves away from the first connecting block 31, it drives the clamping block 341 to move together, and the sorting column 21 and the liquid bottle 22 automatically separate from the clamping piece 345 on the same side.

[0091] In one embodiment, third slide grooves 352 are provided on the side surfaces of the second connection block 35 corresponding to the two clamping members 345. The clamping member 345 is slidably matched with the third slide groove 352 on the same side. Specifically, the overall shape of the clamping member 345 is similar to a "Z" character, and a portion of the clamping member 345 is slidably matched with the third slide groove 352. The third slide groove 352 is open at one end, so that the clamping member 345 can slide in and out of the third slide groove 352. Through the sliding match between the clamping member 345 and the third slide groove 352, the second connection block and the first connection block 31 can maintain a stable sliding match, thereby preventing the second connection block 35 from rotating, so that the end of the limit rod 36 on the second connection block 35 can be stably located in the corresponding second slide groove 13.

[0092] In one embodiment, fifth holes 353 are respectively provided on the side surfaces of the second connecting block 35 facing the two mounting rings 11. The opening of the fifth hole 353 is provided with a limit ring 354 that contracts inward. One end of the limit rod 36 abuts against the second slide groove 13 on the same side, and the other end of the limit rod 36 passes through the limit ring 354 and is connected to a stopper 355. The stopper 355 abuts against the limit ring 354. A third spring 356 is provided in the fifth hole 353. One end of the third spring 356 abuts against the stopper 355, and the other end of the third spring 356 abuts against the bottom of the fifth hole 353. In addition, the limit rod 36 is slidably matched with the inner wall of the limit ring 354, and the stopper 355 is slidably matched with the inner wall of the fifth hole 353.

[0093] In the description of the present invention, a large number of specific details are described. However, it is understood that embodiments of the present invention can be practiced without these specific details. In some instances, well-known methods, structures and techniques are not shown in detail so as not to obscure the understanding of this description.

[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A cell sorting device, characterized in that: include: Rack (10); A sorting assembly (20) comprising a sorting column (21) and a liquid receiving bottle (22) arranged vertically; as well as A plurality of clamping mechanisms (30) having a magnetic field, arranged on the frame (10) and used to clamp the sorting assembly (20), and capable of being retained on the frame (10) and also capable of being rotated in a vertical plane; The sorting column (21) has a liquid inlet end (211) and a liquid outlet end (212). The sorting column (21) is filled with iron beads, and the iron beads are retained in the sorting column (21). The liquid inlet end (211) of the sorting column (21) can be closed; one end of the liquid receiving bottle (22) is open, and the open end (221) of the liquid receiving bottle (22) can be sealed and connected to the liquid outlet end (212) of the sorting column (21).

2. A cell sorting device as claimed in claim 1, characterized in that: Also includes: A motor (40) is arranged on the frame (10); a connecting member (41) rotatably disposed on the frame (10) and connected to an output shaft of the motor (40); and A plurality of drive rings (42), coaxial with the output shaft of the motor (40) and connected to the connecting member (41); The clamping mechanism (30) corresponds to the driving ring (42) in a one-to-one manner; the clamping mechanism (30) can be kept stationary on the frame (10); and the clamping mechanism (30) can be driven by the corresponding driving ring (42) to rotate.

3. A cell sorting device as claimed in claim 2, characterized in that: The frame (10) comprises a plurality of pairs of mounting rings (11) sleeved outside the driving ring (42); the plurality of pairs of mounting rings (11) correspond one-to-one to the plurality of driving rings (42); the two mounting rings (11) in each pair of mounting rings (11) are symmetrically arranged with each other; an annular first sliding groove (12) is provided on the opposite side of each pair of mounting rings (11); and a plurality of evenly distributed first holes (421) are provided on the side wall of the driving ring (42); The clamping mechanism (30) comprises: A first connecting block (31) located between each pair of mounting rings (11); Two arc-shaped sliding blocks (32), respectively connected to two ends of the first connecting block (31) and slidably matched with the first sliding groove (12) on the same side; a connecting rod (33) slidably disposed on the first connecting block (31) and slidably inserted into the first hole (421) on the corresponding driving ring (42); and The clamping assembly (34) is used to clamp the sorting assembly (20).

4. A cell sorting device as claimed in claim 3, characterized in that: An annular second slide groove (13) is also provided on the opposite side of each pair of mounting rings (11); the second slide groove (13) is located outside the first slide groove (12); the second slide groove (13) comprises: Arc segment (131); an inclined section (132) having a deeper end and a shallower end, the shallower end of which is connected to one end of the arc section (131), the depth of the shallower end of which is greater than the depth of the arc section (131), and the distance between the deeper end of which and the axis of the mounting ring (11) is greater than the distance between the shallower end of which and the axis of the mounting ring (11); and a horizontal section (133) extending in a horizontal direction, having a deeper end and a shallower end, wherein the deeper end is connected to the deeper end of the inclined section (132), and the depth of the deeper end is greater than the depth of the deeper end of the inclined section (132), and the shallower end is connected to the other end of the circular arc section (131), and the depth of the shallower end is less than the depth of the circular arc section (131); The clamping mechanism (30) further comprises: A second connecting block (35) is slidably disposed on the first connecting block (31), and is movable along the radial direction of the driving ring (42), thereby driving the connecting rod (33) to move; and Two limiting rods (36) are slidably arranged on both sides of the second connecting block (35), and ends thereof can elastically abut against the second sliding groove (13) on the same side.

5. A cell sorting device as claimed in claim 4, characterized in that: A second hole (311) is provided on a surface of the first connection block (31) facing away from the drive ring (42); a coaxial and penetrating third hole (312) is provided at the bottom of the second hole (311); a fourth hole (351) is provided on a surface of the second connection block (35) facing the first connection block (31); one end of the connection rod (33) is located in the fourth hole (351), and the other end of the connection rod (33) passes through the second hole (311) and the third hole (312) and then faces the drive ring (42); The clamping mechanism (30) further comprises: A limit block (37) connected to one end of the connecting rod (33), located in the fourth hole (351) and slidably engaged with the fourth hole (351) along the axial direction; a first spring (38) sleeved outside the connecting rod (33) and located in the second hole (311), with one end of the first spring abutting against the bottom of the second hole (311) and the other end of the first spring abutting against the limit block (37); and A second spring (39) is located in the fourth hole (351), one end of the second spring abuts against the limit block (37) and the other end of the second spring abuts against the bottom of the fourth hole (351); The first spring (38) and the second spring (39) are respectively located on two sides of the limiting block (37).

6. A cell sorting device as claimed in claim 5, characterized in that: The clamping assembly (34) comprises a clamping block (341); the clamping block (341) is connected to an end of the second connecting block (35) facing away from the first connecting block (31); the clamping block (341) is provided with a first funnel hole (342) and a second funnel hole (343) symmetrically arranged up and down; the first funnel hole (342) and the second funnel hole (343) are connected by a connecting hole (344); the first funnel hole (342) is used to place a sorting column (21); and the second funnel hole (343) is used to place a liquid receiving bottle (22).

7. A cell sorting device as claimed in claim 6, characterized in that: The clamping assembly (34) further comprises two clamping members (345) arranged symmetrically with each other; the two clamping members (345) are both connected to the first connecting block (31); and the ends of the two clamping members (345) away from the first connecting block (31) can respectively abut against the liquid inlet end (211) of the separation column (21) and the closed end of the liquid receiving bottle (22).

8. A cell sorting device as claimed in claim 7, characterized in that: A third sliding groove (352) is provided on the side surfaces of the second connecting block (35) corresponding to the two clamping members (345); the clamping members (345) are slidably matched with the third sliding groove (352) on the same side.

9. A cell sorting device as claimed in claim 8, characterized in that: The second connecting block (35) is provided with a fifth hole (353) on the side facing the two mounting rings (11); the opening of the fifth hole (353) is provided with a limiting ring (354) that contracts inwardly; one end of the limiting rod (36) abuts against the second sliding groove (13) on the same side, and the other end of the limiting rod (36) passes through the limiting ring (354) and is connected to a stopper (355); the stopper (355) abuts against the limiting ring (354); a third spring (356) is provided in the fifth hole (353); one end of the third spring (356) abuts against the stopper (355), and the other end of the third spring (356) abuts against the bottom of the fifth hole (353).

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