A feed for growing water buffaloes and a method for preparing the same

Abstract

The present application relates to the technical field of animal feed. The present application provides a kind of growing period buffalo feed and its preparation method, the feed includes the following weight parts of component: total mixed silage feed 60-90 parts, fermented wu citrus peel 8-12 parts, fermented passion fruit peel 12-18 parts.Wu citrus peel residue and passion fruit peel residue of the present application are fermented after containing bacillus circulans, fructophilic lactic acid bacteria, sharp's bacteria complex lactic acid bacteria and complex enzyme containing laccase, cellulase, pectinase, glucose oxidase, and mixed with total mixed silage fermented by complex lactic acid bacteria, the obtained feed can promote the growth of growing period buffalo, and has obvious inhibitory effect on diarrhea of growing period buffalo.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of animal feed, in particular to a kind of growing period buffalo feed and preparation method thereof. BACKGROUND

[0002] Growing cattle generally refers to 7-18 months old replacement cattle, and growing cattle is generally divided into two stages, the first stage is 6-12 months old, and the second stage is 13-18 months old. The cows in these two stages must be correctly fed according to their age growth characteristics and required nutrition to achieve the goal of early breeding and reproduction. The management of growing cattle directly affects the reproduction of cows and future production, so how to do a good job in the feeding management of growing cattle is an important key to raising good cattle in a farm.

[0003] At present, there are problems such as low utilization rate of forage, unscientific collocation, slow weight gain of cattle and the like in buffalo breeding. Low nutritional level of daily ration will affect the growth performance of beef cattle, and high nutritional level of daily ration will easily lead to that nutrients cannot be completely absorbed and are discharged out of the body with feces or urine, and pollute the ecological environment.

[0004] In the face of rising feed costs, selecting local characteristic unconventional feed resources as buffalo feed can achieve cost reduction and efficiency increase. However, agricultural and sideline products often have characteristics such as low digestibility, high water content and easy spoilage, difficulty in storage, strong seasonality and the like. Guangxi is a major fruit producing area, and there are a large amount of fruit residue by-products to be treated locally. Direct disposal as waste will cause a lot of pollution and resource waste, and use as buffalo feed can reduce the cost of buffalo breeding. How to use fruit residue by-products to prepare a feed suitable for growing period buffalo is a technical problem to be solved by the present application. SUMMARY

[0005] The present application aims to provide a kind of growing period buffalo feed and preparation method thereof, can promote the growth of growing period buffalo, reduce diarrhea.

[0006] In order to achieve the above-mentioned application purpose, the present application provides the following technical scheme:

[0007] The present application provides a kind of growing period buffalo feed, comprising the following components by weight fraction: total mixed silage feed 60-90 parts, fermented Wohwan peel 8-12 parts, fermented passion fruit peel 12-18 parts.

[0008] As preferred, the preparation method of the total mixed silage feed is:

[0009] (1) the corn straw, peanut vine is crushed and mixed with concentrate evenly, and the mixture 1 is obtained;

[0010] (2) adding compound lactic acid bacteria to the mixture 1, and obtaining total mixed silage feed after ensiling.

[0011] Preferably, the mass ratio of the corn stalks, peanut vines and concentrate in step (1) is 20-30:20-30:20-30;

[0012] The concentrate is composed of the following components in mass fraction: corn flour 55-65 parts, soybean meal 20-30 parts, wheat bran 9-13 parts, sodium chloride 0.5-1.5 parts, calcium hydrogen phosphate 1.0-1.4 parts, calcium carbonate 0.2-0.6 parts, selenium yeast 0.01-0.06 parts, vitamin E 0.01-0.06 parts, mold inhibitor 0.2-0.5 parts, antioxidant 0.01-0.04 parts, premix 0.5-1.5 parts;

[0013] The premix contains, per kg of premix, vitamin E 2000-4000 IU, vitamin D 120000-180000 IU, vitamin A 450000-550000 IU, Cu 0.8-1.8 g, Fe 3.0-5.0 g, Mn 2.0-4.0 g, I 60-100 mg, Zn 4.0-8.0 g, Co 60-100 mg, Se 30-70 mg.

[0014] Preferably, the concentrate is further added with anamaria extract, and the addition ratio of the anamaria extract is 0.5-1.5 parts per 1000 parts of concentrate.

[0015] Preferably, the mass ratio of the mixture 1 to the compound lactic acid bacteria in step (2) is 1000:1-3, and the ensiling time is 20-40 days.

[0016] Preferably, the preparation method of the fermented citrus peel is as follows: the compound lactic acid bacteria and the compound enzyme are added to the citrus peel residue, the mixture is uniformly mixed and then stacked to obtain the fermented citrus peel.

[0017] The preparation method of the fermented guava peel is as follows: the compound lactic acid bacteria and the compound enzyme are added to the guava peel residue, the mixture is uniformly mixed and then stacked to obtain the fermented guava peel.

[0018] Preferably, the mass ratio of the citrus peel residue, the compound lactic acid bacteria and the compound enzyme is 1000:0.5-1.5:0.5-1.5.

[0019] The mass ratio of the guava peel residue, the compound lactic acid bacteria and the compound enzyme is 1000:0.5-1.5:0.5-1.5.

[0020] Preferably, the compound lactic acid bacteria comprises the following components in mass fraction: 2-4 parts of Lysinibacillus fusiformis, 3-5 parts of fructophilic lactic acid bacteria, 2-4 parts of Sharpea.

[0021] The effective viable cell number of each component in the complex lactic acid bacteria is as follows: 1.30*10 10 ~1.40*10 10 cfu / g, fructophilic lactic acid bacteria 1.35*10 10 ~1.45*10 10 cfu / g, Sharpe's bacteria 1.35*10 10 ~1.45*10 10 cfu / g.

[0022] As preferred, the complex enzyme comprises the following components in mass fraction: 2-4 parts of laccase, 7-9 parts of cellulase, 6-8 parts of pectinase, 1-3 parts of glucose oxidase;

[0023] The effective activity of each component in the complex enzyme is as follows: laccase 800-1200 U / g, cellulase 48000-52000 U / g, pectinase 48000-52000 U / g, glucose oxidase 998000-102000 U / g.

[0024] The application also provides a preparation method of the growing period buffalo feed, and the steps are as follows: mixing the total mixed silage feed, fermented Wampee peel and fermented passion fruit peel to obtain the growing period buffalo feed.

[0025] The application has the following beneficial effects:

[0026] The application provides a growing period buffalo feed and a preparation method thereof, and the feed comprises the following components in weight fraction: 60-90 parts of total mixed silage feed, 8-12 parts of fermented Wampee peel and 12-18 parts of fermented passion fruit peel. The Wampee peel residue and the passion fruit peel residue are fermented by complex lactic acid bacteria containing Leuconostoc dextranicum, fructophilic lactic acid bacteria and Sharpe's bacteria and complex enzyme containing laccase, cellulase, pectinase and glucose oxidase, and are mixed with the total mixed silage feed fermented by the complex lactic acid bacteria, so that the synergistic effect between the bacteria and the enzymes is achieved, the lignin degradation rate is improved, the feed energy and the feed digestibility are improved, the use amount of concentrated feed is greatly saved, and the cost of the livestock and poultry industry is greatly reduced. The obtained feed can promote the growth of the growing period buffalo, improve the antioxidant capacity of the growing period buffalo, and obviously inhibit the diarrhea of the growing period buffalo. DETAILED DESCRIPTION

[0027] The application provides a growing period buffalo feed, which comprises the following components in weight fraction: 60-90 parts of total mixed silage feed, 8-12 parts of fermented Wampee peel and 12-18 parts of fermented passion fruit peel.

[0028] In this invention, the total mixed silage is preferably 70-80 parts, and more preferably 75 parts.

[0029] In this invention, the fermented Wogan peel is preferably 9 to 11 parts, and more preferably 10 parts.

[0030] In this invention, the fermented passion fruit peel is preferably 14 to 16 parts, and more preferably 15 parts.

[0031] In this invention, the method for preparing the total mixed silage is as follows:

[0032] (1) Crush corn stalks and peanut vines and mix them evenly with refined materials to obtain mixture 1;

[0033] (2) Add compound lactic acid bacteria to the mixture 1, and after ensiling, obtain a total mixed silage.

[0034] In this invention, the particle size of the crushed corn stalks and peanut vines is preferably 1-4 mm, more preferably 2-3 mm, and even more preferably 2 mm.

[0035] In this invention, the preferred mass ratio of corn stalks, peanut vines and concentrate in step (1) is 20~30:20~30:20~30, more preferably 23~26:23~26:23~26, and even more preferably 24~25:24~25:24~25.

[0036] In this invention, the concentrate is composed of the following components in parts by weight: 55-65 parts corn flour, 20-30 parts soybean meal, 9-13 parts wheat bran, 0.5-1.5 parts sodium chloride, 1.0-1.4 parts dicalcium phosphate, 0.2-0.6 parts calcium carbonate, 0.01-0.06 parts yeast selenium, 0.01-0.06 parts vitamin E, 0.2-0.5 parts mycotoxin binder, 0.01-0.04 parts antioxidant, and 0.5-1.5 parts premix.

[0037] In this invention, the corn flour is preferably 58-62 parts, more preferably 59-61 parts, and even more preferably 60 parts.

[0038] In this invention, the soybean meal is preferably 23-27 parts, more preferably 24-26 parts, and even more preferably 25 parts.

[0039] In this invention, the wheat bran is preferably 10 to 12 parts, and more preferably 11 parts.

[0040] In this invention, the sodium chloride is preferably 0.8 to 1.2 parts, and more preferably 1 part.

[0041] In the present application, the calcium hydrogen phosphate is preferably 1.1 to 1.3 parts, and further preferably 1.2 parts.

[0042] In the present application, the calcium carbonate is preferably 0.3 to 0.5 parts, and further preferably 0.4 parts.

[0043] In the present application, the selenium yeast is preferably 0.03 to 0.05 parts, and further preferably 0.04 parts.

[0044] In the present application, the vitamin E is preferably 0.03 to 0.05 parts, and further preferably 0.04 parts.

[0045] In the present application, the mold inhibitor is preferably 0.03 to 0.04 parts, and further preferably 0.03 parts.

[0046] In the present application, the antioxidant is preferably 0.02 to 0.03 parts, and further preferably 0.03 parts.

[0047] In the present application, the premix is preferably 0.8 to 1.2 parts, and further preferably 1.0 parts.

[0048] In the present application, the premix contains, per 1 kg of the premix, 2000 to 4000 IU of vitamin E, 120000 to 180000 IU of vitamin D1, 450000 to 550000 IU of vitamin A, 0.8 to 1.8 g of Cu, 3.0 to 5.0 g of Fe, 2.0 to 4.0 g of Mn, 60 to 100 mg of I, 4.0 to 8.0 g of Zn, 60 to 100 mg of Co, and 30 to 70 mg of Se.

[0049] In the present application, the content of the vitamin E is preferably 2500 to 3500 IU, and further preferably 3000 IU.

[0050] In the present application, the content of the vitamin D is preferably 130000 to 170000 IU, and further preferably 140000 to 160000 IU, and still further preferably 150000 IU.

[0051] In the present application, the content of the vitamin A is preferably 480000 to 520000 IU, and further preferably 490000 to 510000 IU, and still further preferably 500000 IU.

[0052] In the present application, the content of the Cu is preferably 1.0 to 1.6 g, and further preferably 1.2 to 1.4 g, and still further preferably 1.3 g.

[0053] In the present application, the Fe content is preferably 3.5-4.5 g, and further preferably 4.0 g.

[0054] In the present application, the Mn content is preferably 2.5-3.5 g, and further preferably 3.0 g.

[0055] In the present application, the I content is preferably 70-90 mg, and further preferably 80 mg.

[0056] In the present application, the Zn content is preferably 5.0-7.0 g, and further preferably 6.0 g.

[0057] In the present application, the Co content is preferably 70-90 mg, and further preferably 80 mg.

[0058] In the present application, the Se content is preferably 40-60 mg, and further preferably 50 mg.

[0059] In the present application, the concentrate is also added with anamni extract, and the addition ratio of the anamni extract is 0.5-1.5 parts of anamni extract per 1000 parts of concentrate, preferably 0.8-1.2 parts of anamni extract per 1000 parts of concentrate, and further preferably 1 part of anamni extract per 1000 parts of concentrate.

[0060] In the present application, the mass ratio of the mixture 1 to the compound lactic acid bacteria in step (2) is 1000:1-3, preferably 1000:1.5-2.5, and further preferably 1000:2, and the ensiling time is 20-40 days, preferably 25-35 days, and further preferably 30 days.

[0061] In the present application, the preparation method of the fermented wampee peel is as follows: adding compound lactic acid bacteria and compound enzyme into wampee peel residue, mixing uniformly, and stacking to obtain fermented wampee peel.

[0062] In the present application, the stacking time of the wampee peel residue, compound lactic acid bacteria, and compound enzyme is preferably 10-30 days, further preferably 15-25 days, and still further preferably 20 days.

[0063] In the present application, the preparation method of the fermented guava peel is as follows: adding compound lactic acid bacteria and compound enzyme into guava peel residue, mixing uniformly, and stacking to obtain fermented guava peel.

[0064] In the present application, the stacking time of the guava peel residue, compound lactic acid bacteria, and compound enzyme is preferably 10-30 days, further preferably 15-25 days, and still further preferably 20 days.

[0065] In the present application, the mass ratio of the orange peel residue, the composite lactic acid bacteria and the composite enzyme is 1000:0.5-1.5:0.5-1.5, preferably 1000:0.8-1.2:0.8-1.2, and further preferably 1000:1:1.

[0066] In the present application, the mass ratio of the orange peel residue, the composite lactic acid bacteria and the composite enzyme is 1000:0.5-1.5:0.5-1.5, preferably 1000:0.8-1.2:0.8-1.2, and further preferably 1000:1:1.

[0067] In the present application, the composite lactic acid bacteria comprises the following components in the mass fraction: 2-4 parts of Lactobacillus mucosus, 3-5 parts of Fructobacillus sp., and 2-4 parts of Weisella sp.

[0068] In the present application, the Lactobacillus mucosus is preferably 2.5-3.5 parts, and further preferably 3.0 parts.

[0069] In the present application, the Fructobacillus sp. is preferably 3.5-4.5 parts, and further preferably 4.0 parts.

[0070] In the present application, the Weisella sp. is preferably 2.5-3.5 parts, and further preferably 3.0 parts.

[0071] In the present application, the effective viable cell number of each component in the composite lactic acid bacteria is as follows: 1.30×10 10 ~1.40×10 10 cfu / g for Lactobacillus mucosus, 1.35×10 10 ~1.45×10 10 cfu / g for Fructobacillus sp., and 1.35×10 10 ~1.45×10 10 cfu / g for Weisella sp.

[0072] In the present application, the effective viable cell number of the Lactobacillus mucosus is preferably 1.34×10 10 ~1.36×10 10 cfu / g, and further preferably 1.35×10 10 cfu / g

[0073] In the present application, the effective viable cell number of the Fructobacillus sp. is preferably 1.38×10 10 ~1.42×10 10 cfu / g, and further preferably 1.40×10 10 cfu / g.

[0074] In the present application, the effective viable cell number of the Weisella sp. is preferably 1.38×10 101.42x10 10 1.40x10 10 cfu / g, further preferably 1.40x10

[0075] In the present application, the complex enzyme comprises the following components in mass fraction: 2-4 parts of laccase, 7-9 parts of cellulase, 6-8 parts of pectinase, 1-3 parts of glucose oxidase.

[0076] In the present application, the laccase is preferably 2.5-3.5 parts, further preferably 3.0 parts.

[0077] In the present application, the cellulase is preferably 7.5-8.5 parts, further preferably 8.0 parts.

[0078] In the present application, the pectinase is preferably 6.2-7.8 parts, further preferably 7.0 parts.

[0079] In the present application, the glucose oxidase is preferably 1.5-2.5 parts, further preferably 2.0 parts.

[0080] In the present application, the effective activity of each component in the complex enzyme is as follows: laccase 800-1200 U / g, cellulase 48000-52000 U / g, pectinase 48000-52000 U / g, glucose oxidase 998000-102000 U / g.

[0081] In the present application, the effective activity of the laccase is preferably 900-1100 U / g, further preferably 1000 U / g.

[0082] In the present application, the effective activity of the cellulase is preferably 49000-51000 U / g, further preferably 50000 U / g.

[0083] In the present application, the effective activity of the pectinase is preferably 49000-51000 U / g, further preferably 50000 U / g.

[0084] In the present application, the effective activity of the glucose oxidase is preferably 999000-101000 U / g, further preferably 100000 U / g.

[0085] The present application also provides a preparation method of the growing period buffalo feed, comprising the following steps: mixing the total mixed silage feed, fermented orange peel and fermented passion fruit peel to obtain the growing period buffalo feed.

[0086] The technical solutions provided by the present application will be described in detail below in conjunction with the embodiments, but they should not be understood as limiting the scope of protection of the present application.

[0087] Example 1 Preparation of feed for growing water buffalo

[0088] I. Raw material composition of the feed

[0089] Two kinds of feed were respectively configured, 1) basic feed; 2) test feed, the specific formulations of the two kinds of feed are shown in Table 1 and Table 2.

[0090] Among them, the premix in Table 2 contains: 3000 IU of vitamin E, 150 000 IU of vitamin D, 500 000 IU of vitamin A, 1.3 g of Cu, 4.0 g of Fe, 3.0 g of Mn, 80 mg of I, 6.0 g of Zn, 80 mg of Co, 50 mg of Se per kg.

[0091] In the 2) test feed, 1 g of oenanthe javanica extract was also added per kg of concentrate.

[0092] Table 1 Composition and nutrient level of the feed (dry matter basis)

[0093]

[0094] Table 2 Concentrate formula in the feed formula (dry matter basis)

[0095]

[0096] II. Preparation of each component in the test feed

[0097] In the test feed, the preparation steps of the total mixed silage feed, fermented wampee peel and fermented passion fruit peel are as follows:

[0098] 1) Preparation of total mixed silage feed

[0099] Corn straw and peanut vine were crushed to about 2 cm, corn straw: peanut vine: fattening cattle concentrate was mixed uniformly at a ratio of 1:1:1 of dry matter, 2 kg / ton of compound lactic acid bacteria was added, and ensiled for 30 days to obtain the total mixed silage feed.

[0100] 2) Preparation of fermented wampee peel

[0101] The wampee peel residue was added with 1 kg / ton of compound lactic acid bacteria and 1 kg / ton of compound enzyme, and then mixed uniformly and stacked for 10 days.

[0102] 3) Preparation of fermented passion fruit peel

[0103] The wampee peel residue was added with 1 kg / ton of compound lactic acid bacteria and 1 kg / ton of compound enzyme, and then mixed uniformly and stacked for 10 days.

[0104] The composite enzyme involved is composed of: laccase (1000 U / g) 15%, cellulase (50000 U / g) 40%, pectinase (50000 U / g) 35%, glucose oxidase (100000 U / g) 10%

[0105] The composite lactic acid bacteria involved is composed of: Leclercia adecarum Lachnobacterium (1.35 x 10 10 cfu / g) 30%, fructophilic lactic acid bacteria Fructobacillus (1.39 x 10 10 cfu / g) 40%, Sharpea vallontia Sharpea (1.38 x 10 10 cfu / g) 30%, the Leclercia adecarum, fructophilic lactic acid bacteria, Sharpea vallontia are all purchased from BioVector NTCC strain preservation center.

[0106] After mixing according to the dry matter ratio of 75:10:15 of the total mixed silage: fermented Wuchang orange peel: fermented passion fruit peel, further mixing uniformly by using a feed mixer, the test feed is obtained.

[0107] III. Preparation of the basal feed

[0108] Corn stalks and peanut vines are crushed to about 2 cm, corn stalks, peanut vines, and fattening cattle concentrate are mixed according to the proportions (dry matter) recorded in Table 1, and further mixed uniformly by using a feed mixer, and the basal feed is obtained.

[0109] Example 2 Influence of different feeds on the growth performance of growing buffalo

[0110] I. Experimental design

[0111] Select 60 heads of 12-month-old growing buffalo with close body weight (about 280 kg), average and randomly divide into 2 treatments, feed the two feeds in Example 1, the basal feed and the test feed. In each treatment, 30 buffalo are randomly divided into 3 groups, 10 heads each, recorded as 3 replicates, continuously fed for 8 weeks, including 1 week of pre-feeding and 7 weeks of test period.

[0112] II. Feeding management

[0113] During the test period, the feeds are fed once a day, free feeding and drinking water, and ensuring that there is leftover feed after each buffalo feeds. Weighing is carried out in the morning before feeding at the 0th week (at the beginning of the test), the 1st week and the 8th week of the test, and the amount of leftover feed is collected and weighed every day, and the weighing is carried out at 8:00 the night before, and the weight is measured at 8:00 the next morning on an empty stomach, and the daily weight gain, feed intake and feed conversion ratio are calculated through the obtained data after the test is completed.

[0114] III. Feed sample collection and detection

[0115] The feed of each group of test cattle was sampled once a week after being mixed evenly. The feed sample was dried at 65°C for 72 h, crushed, and then detected for moisture, protein, fiber, energy, ash, and other nutrient indicators. The nitrogen content was detected by the Kjeldahl method, the energy was determined by an oxygen bomb calorimeter, and the fiber was detected by an ANKOM A2000I fiber detector.

[0116] IV. Fecal sample collection and detection

[0117] The feces were collected by the endogenous indicator method. The collected feces were weighed, stirred evenly, and then about 1% of the total amount was taken as a sample. The sample was dried at 65°C for 72 h, crushed, and then detected for moisture, fiber, ash, and other nutrient indicators. Another sample was taken for protein content detection, and 10% sulfuric acid was added to it in a geometric progression to detect the protein content.

[0118] Apparent digestibility of a certain nutrient (%) = (total amount of ingested feed x content of the nutrient in the feed - excretion amount x content of the nutrient in the feces) / (total amount of ingested feed x content of the nutrient in the feed) x 100

[0119] The effects of different feeds on the growth performance of growing buffalo are shown in Table 3.

[0120] Table 3 Effects of different feeds on the growth performance of growing buffalo

[0121]

[0122] Note: The right shoulder mark of the data indicates that there is a significant difference between the same row data ( P <0.05), and no shoulder mark indicates that there is no significant difference between the same row data ( P >0.05).

[0123] As shown in Table 3, the test feed significantly increased the daily weight gain and dry matter intake of buffalo ( P <0.05), and the difference in feed conversion rate between the two feeds was not significant ( P >0.05). Compared with the basic feed, the test feed significantly improved the digestibility of crude protein, neutral detergent fiber, and acid detergent fiber ( P <0.05).

[0124] Example 3 Diarrhea detection

[0125] During the test period described in Example 2, the diarrhea condition of each buffalo was recorded, and was divided into no diarrhea, mild diarrhea, moderate diarrhea, and severe diarrhea. The average diarrhea time of growing buffalo was calculated.

[0126] The diarrhea judgment criteria are as follows:

[0127] No diarrhea: buffalo feces are normal, without obvious thinning or watery stool;

[0128] Mild diarrhea: buffalo feces become slightly thin, but there are no obvious symptoms of diarrhea, and the feeding, water source and activity are slightly affected;

[0129] Moderate diarrhea: buffalo feces are obviously thin, the number of loose stools per day increases, and obvious symptoms of diarrhea such as decreased appetite, poor mental state, etc. appear;

[0130] Severe diarrhea: buffalo feces are very thin, may have blood or other abnormal substances, frequent diarrhea per day, and buffalo shows obvious weakness, dehydration, digestive problems and other serious complications.

[0131] Table 4 Influence of different feeds on diarrhea of growing buffalo

[0132]

[0133] As can be seen from Table 4, compared with the basic feed feeding, the experimental feed can significantly reduce the diarrhea degree and diarrhea time of the growing buffalo, and improve the diarrhea problem of the growing buffalo.

[0134] Example 4 Antioxidant detection

[0135] During the test described in Example 2, 15 mL of blood was collected from the tail vein of the buffalo at the beginning of the test and before morning feeding on the last day of the test, and the serum was separated by low-temperature centrifuge at 3000 r / min for 15 min at 4°C, and stored at -80°C for detection. The superoxide dismutase (SOD) activity detection kit D799593 was used to determine the superoxide dismutase (SOD) activity in the serum, the glutathione peroxidase (GPX) activity detection kit D799617 was used to determine the glutathione peroxidase (GSH-Px) activity, the malondialdehyde (MDA) content detection kit D799761 was used to determine the malondialdehyde (MDA) content, and the total antioxidant capacity detection kit D799275 was used to determine the total antioxidant capacity (T-AOC). The above kits were purchased from Shengong Bioengineering (Shanghai) Co., Ltd., and the determination results are shown in Table 5.

[0136] Table 5 Influence of different feeds on serum antioxidant indexes of growing buffalo

[0137]

[0138] Note: The right shoulder mark on the data indicates that there is a significant difference between the same row data (P<0.05). P <0.05).

[0139] Table 5 shows that after 8 weeks of the experiment, there were no significant differences in serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) levels in the growing buffalo fed the basal diet compared to the start of the experiment. After 8 weeks of feeding the experimental diet, compared to the control group fed the basal diet, serum levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) were all significantly increased. P< 0.05); malondialdehyde (MDA) content was significantly reduced ( P< The value of 0.05 indicates that feeding the experimental feed significantly improved the antioxidant capacity of growing buffalo.

[0140] As can be seen from the above embodiments, the present invention provides a feed for growing buffalo and its preparation method. The feed for growing buffalo comprises 60-90 parts of total mixed silage, 8-12 parts of fermented Wogan orange peel, and 12-18 parts of fermented passion fruit peel. The Wogan orange peel residue and passion fruit peel residue are fermented with a complex of lactic acid bacteria containing Trichoderma, fructose-loving lactic acid bacteria, and Sharpe, and a complex of enzymes containing laccase, cellulase, pectinase, and glucose oxidase. After fermentation, they are mixed with the total mixed silage fermented with the complex lactic acid bacteria. The resulting feed can promote the growth of growing buffalo, improve the antioxidant capacity of the buffalo, and reduce the occurrence of buffalo diarrhea.

[0141] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A feed for growing water buffalo characterized by, The feed comprises the following components in parts by weight: 60-90 parts of total mixed silage, 8-12 parts of fermented Wohwan peel, and 12-18 parts of fermented passion fruit peel; The preparation method of the total mixed silage is as follows: (1) crushing corn stalks and peanut vines, and uniformly mixing the crushed corn stalks and peanut vines with concentrates to obtain a mixture 1; (2) adding compound lactic acid bacteria to the mixture 1, and obtaining total mixed silage after ensiling; In step (2), the mass ratio of the mixture 1 to the compound lactic acid bacteria is 1000:1-3, and the ensiling time is 20-40 days; The preparation method of the fermented Wohwan peel is as follows: adding compound lactic acid bacteria and compound enzymes to Wohwan peel residue, uniformly mixing, and stacking to obtain fermented Wohwan peel; The mass ratio of the Wohwan peel residue, the compound lactic acid bacteria, and the compound enzymes is 1000:0.5-1.5:0.5-1.5; The preparation method of the fermented passion fruit peel is as follows: adding compound lactic acid bacteria and compound enzymes to passion fruit peel residue, uniformly mixing, and stacking to obtain fermented passion fruit peel; The mass ratio of the passion fruit peel residue, the compound lactic acid bacteria, and the compound enzymes is 1000:0.5-1.5:0.5-1.5; The complex lactic acid bacteria comprises the following components in mass fraction: 2-4 parts of Lachnobacterium, 3-5 parts of Fructobacillus, 2-4 parts of Sharpea Sharpea ; The effective viable cell number of each component in the complex lactic acid bacteria is as follows: Lachnospira 1.30 x 10 10 ~1.40 x 10 10 cfu / g, Fructophilic lactic acid bacteria 1.35 x 10 10 ~1.45 x 10 10 cfu / g, Sharpea 1.35 x 10 10 ~1.45 x 10 10 cfu / g; The compound enzymes comprise the following components in parts by mass: 2-4 parts of laccase, 7-9 parts of cellulase, 6-8 parts of pectinase, and 1-3 parts of glucose oxidase; The effective activities of the components in the compound enzymes are as follows: laccase 800-1200 U / g, cellulase 48000-52000 U / g, pectinase 48000-52000 U / g, and glucose oxidase 998000-102000 U / g.

2. The feed for growing water buffalo according to claim 1, characterized by, In step (1), the mass ratio of the corn stalks, the peanut vines, and the concentrates is 20-30:20-30:20-30; The concentrates are composed of the following components in parts by mass: 55-65 parts of corn flour, 20-30 parts of soybean meal, 9-13 parts of wheat bran, 0.5-1.5 parts of sodium chloride, 1.0-1.4 parts of calcium hydrogen phosphate, 0.2-0.6 parts of calcium carbonate, 0.01-0.06 parts of selenium yeast, 0.01-0.06 parts of vitamin E, 0.2-0.5 parts of mold inhibitor, 0.01-0.04 parts of antioxidant, and 0.5-1.5 parts of premix; In the premix, 1 kg of the premix contains 2000-4000 IU of vitamin E, 120000-180000 IU of vitamin D, 450000-550000 IU of vitamin A, 0.8-1.8 g of Cu, 3.0-5.0 g of Fe, 2.0-4.0 g of Mn, 60-100 mg of I, 4.0-8.0 g of Zn, 60-100 mg of Co, and 30-70 mg of Se.

3. The feed for growing water buffalo according to claim 2, characterized by that, The concentrates further comprise anamni extract, and the addition ratio of the anamni extract is 0.5-1.5 parts of the anamni extract per 1000 parts of the concentrates.

4. The method for preparing the feed for buffalo in growing period according to any one of claims 1 to 3, characterized in that, The steps are as follows: mixing the total mixed silage, the fermented Wohwan peel, and the fermented passion fruit peel to obtain the feed for growing water buffaloes.

Images