Preparation method of Lianzhen Tonglin preparation
By employing dynamic extraction and β-cyclodextrin inclusion technology, the problem of volatile oil loss during the preparation process was solved, thereby improving the efficacy and quality of the Liancan Tonglin preparation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHINEWAY PHARMA GRP LTD
- Filing Date
- 2024-03-25
- Publication Date
- 2026-05-08
AI Technical Summary
In the preparation of Lian Shen Tong Lin preparations, the mixture of volatile oil extracted by steam distillation and water is prone to loss due to sticking to the wall during the cooling and separation process, which affects the efficacy of the finished drug.
A dynamic extraction method combined with β-cyclodextrin inclusion technology was used to extract volatile oils from Atractylodes macrocephala and Acorus tatarinowii using a 35-40 vol% ethanol aqueous solution. The volatile oil powder was then prepared by centrifugal emulsification and spray drying to avoid the loss of volatile oils. At the same time, appropriate ethanol concentration and temperature were selected for reflux extraction of the medicinal materials to ensure the extraction of effective components.
It improves the utilization rate of volatile oils and the efficacy of products, simplifies the process, and enhances the therapeutic effect of formulations.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of pharmaceutical preparation technology, and in particular to a method for preparing a Lian Shen Tong Lin preparation. Background Technology
[0002] Lian Shen Tong Lin preparation is composed of 13 traditional Chinese medicines: Coptis chinensis, Sophora flavescens, Dianthus superbus, Akebia trifoliata, Polygonum aviculare, Gardenia jasminoides, Rheum palmatum, Salvia miltiorrhiza, Dioscorea hypoglauca, Poria cocos, Atractylodes macrocephala, Acorus tatarinowii, and Glycyrrhiza uralensis. It has the effects of clearing heat and removing dampness, promoting urination and relieving strangury. It is used as an adjunct treatment for non-gonococcal urethritis, specifically for those diagnosed by traditional Chinese medicine as having damp-heat accumulation in the lower jiao, with symptoms such as frequent urination, urgency, painful urination, urethral redness, swelling and itching, urethral discharge, red tongue with yellow and greasy coating, and a soft and rapid pulse. This formula has shown significant efficacy and is safe and reliable in clinical use.
[0003] In the preparation of Lian Shen Tong Lin preparations, the volatile oils of Atractylodes macrocephala and Acorus tatarinowii are generally extracted by steam distillation. The volatile oils are then encapsulated with β-cyclodextrin to obtain volatile oil inclusion complexes. Other herbs are extracted, concentrated, dried, and pulverized to obtain fine powder of the extract. Finally, the volatile oil inclusion complexes are mixed with the fine powder of the extract to obtain the final product. However, this method requires extracting the volatile oils before encapsulation. During the subsequent cooling and separation process, the mixture of volatile oils and water extracted by steam distillation may suffer from volatile oil adhesion to the walls, leading to volatile oil loss and affecting the volatile oil content in the finished drug, thus impacting its efficacy. Summary of the Invention
[0004] To address the above problems, this invention provides a method for preparing a Lian Shen Tong Lin preparation.
[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:
[0006] A method for preparing a Lian Shen Tong Lin preparation, the method comprising the following steps:
[0007] Take Atractylodes macrocephala and Acorus tatarinowii powder, add 35~40 vol% ethanol aqueous solution and 0.006~0.008 times the weight of β-cyclodextrin, extract dynamically, filter, and obtain volatile oil liquid and Atractylodes macrocephala and Acorus tatarinowii residue;
[0008] The volatile oil solution was concentrated under reduced pressure, centrifuged and emulsified through a colloid mill, then a binder was added, mixed well, and dried to obtain volatile oil paste powder.
[0009] Take the residue of Atractylodes macrocephala and Acorus tatarinowii, add 55-60 vol% ethanol aqueous solution, reflux for extraction, cool to room temperature for solid-liquid separation, recover ethanol from the obtained liquid until there is no alcohol odor, concentrate under reduced pressure, dry and pulverize to obtain Atractylodes macrocephala and Acorus tatarinowii powder.
[0010] Take eight herbs: Aristolochia debilis, Polygonum aviculare, Gardenia jasminoides, Rheum palmatum, Salvia miltiorrhiza, Dioscorea hypoglauca, Poria cocos, and Glycyrrhiza uralensis. Add 55-60 vol% ethanol aqueous solution, reflux to extract, cool to room temperature and filter, recover ethanol until no alcohol taste, concentrate under reduced pressure, dry and pulverize to obtain the powder of the eight herbs.
[0011] Take three herbs: Coptis chinensis, Sophora flavescens, and Dianthus superbus. Add 80-90 vol% ethanol aqueous solution, reflux to extract, filter, recover ethanol until no alcohol taste remains, concentrate under reduced pressure, dry, and pulverize to obtain powder of the three herbs.
[0012] Take volatile oil powder, Atractylodes macrocephala and Acorus tatarinowii powder, eight kinds of medicinal powder and three kinds of medicinal powder and mix them evenly to obtain Lian Shen Tong Lin San (a traditional Chinese medicine formula);
[0013] The Lian Shen Tong Lin powder is one type of the Lian Shen Tong Lin preparation.
[0014] Furthermore, the dynamic extraction temperature is 55~60℃ and the time is 2~3h.
[0015] Furthermore, the vacuum concentration temperature is 55~60℃ and the vacuum degree is -0.08~-0.10MPa.
[0016] Furthermore, after vacuum concentration, the relative density of the concentrated solution is 1.05~1.08.
[0017] Furthermore, the weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to 35-40 vol% ethanol aqueous solution is 1:4-6.
[0018] Furthermore, the weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to the binder is 1:0.0008~0.001.
[0019] Furthermore, the weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to 55-60 vol% ethanol aqueous solution is 1:5-6;
[0020] The weight ratio of the eight herbs to a 55-60 vol% ethanol aqueous solution is 1:5-6.
[0021] Furthermore, the weight ratio of the three herbs to an 80-90 vol% ethanol aqueous solution is 1:5-6.
[0022] Furthermore, the weight ratio of Atractylodes macrocephala, Acorus tatarinowii, Akebia trifoliata, Polygonum aviculare, Gardenia jasminoides, Rheum palmatum, Salvia miltiorrhiza, Dioscorea hypoglauca, Poria cocos, Glycyrrhiza uralensis, Coptis chinensis, Sophora flavescens, and Dianthus superbus is 70:30:90:90:60:80:80:80:90:30:120:90:90.
[0023] Furthermore, the Lian Shen Tong Lin powder is granulated with excipients to obtain Lian Shen Tong Lin granules.
[0024] Lian Shen Tong Lin Granules can be compressed into tablets or capsules.
[0025] The beneficial effects of the preparation method of the Liancan Tonglin preparation of the present invention are as follows:
[0026] This invention utilizes β-cyclodextrin to simultaneously extract and encapsulate the volatile oils from Atractylodes macrocephala and Acorus tatarinowii during the dynamic extraction process. This avoids losses caused by adhesion to equipment during the extraction and separation of volatile oils, improves the utilization rate of volatile oils, and thus enhances the efficacy of the product.
[0027] This invention effectively extracts the active ingredients from the residues of Atractylodes macrocephala and Acorus tatarinowii by selecting appropriate solvents, thereby improving the efficacy of the product.
[0028] This invention utilizes a suitable solvent to extract eight medicinal herbs, including Aristolochia debilis, Polygonum aviculare, and Gardenia jasminoides, thereby effectively extracting their active ingredients and improving the efficacy of the product.
[0029] This invention extracts the active ingredients from three medicinal herbs—Coptis chinensis, Sophora flavescens, and Dianthus superbus—by selecting a suitable solvent, thereby improving the efficacy of the product. Furthermore, the entire extraction process does not require alcohol precipitation, simplifying the process. Detailed Implementation
[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
[0031] Example 1: A method for preparing a ginseng-based laxative.
[0032] This embodiment describes a method for preparing a Lian Shen Tong Lin preparation. The specific preparation process includes the following steps:
[0033] S1. Take 70 kg of Atractylodes macrocephala and 30 kg of Acorus tatarinowii, crush them, and pass them through a 300-400 mesh sieve (in this embodiment, pass them through a 325 mesh sieve) to obtain Atractylodes macrocephala and Acorus tatarinowii powder;
[0034] S2. In a dynamic extraction tank, add 100 kg of Atractylodes macrocephala and Acorus tatarinowii powder, 500 kg of 35 vol% ethanol aqueous solution and 0.7 kg of β-cyclodextrin, and extract dynamically at 60℃ for 2.5 h. After centrifugation and filtration, the resulting extract yields volatile oil liquid and residues of Atractylodes macrocephala and Acorus tatarinowii.
[0035] S3. The volatile oil solution is concentrated under reduced pressure to a relative density of 1.06 (i.e., the relative density of the concentrated solution after concentration is 1.06). The temperature of the reduced pressure concentration is 60℃ and the vacuum degree is -0.09MPa. The solution is then passed through a 100~500 mesh sieve (in this embodiment, it is passed through a 200 mesh sieve) to obtain the volatile oil concentrate.
[0036] S4. The volatile oil concentrate was centrifuged and emulsified using a colloid mill at a speed of 3210 r / min and an emulsification fineness of 30 μm. Then, 0.1 kg of binder (dextrin was used as the binder in this embodiment) was added, stirred and mixed at room temperature, and spray dried to obtain 1.962 kg of volatile oil paste powder (equivalent to 1.162 kg of volatile oil), labeled as G1.
[0037] It should be noted that spray drying was used as the drying method for the volatile oil powder in this embodiment, but it is not intended to limit the drying method of the present invention. Other conventional drying methods can also be used, including but not limited to vacuum drying, spray drying and freeze drying, which will not be described in detail here.
[0038] S5. Take the residue of Atractylodes macrocephala and Acorus tatarinowii, add 500 kg of 60 vol% ethanol aqueous solution, reflux and extract twice, cool to room temperature and centrifuge to separate solid and liquid, filter the obtained liquid, combine the filtrates, no alcohol precipitation is required, recover the ethanol until there is no alcohol taste, concentrate under reduced pressure, dry and pulverize to obtain Atractylodes macrocephala and Acorus tatarinowii powder.
[0039] S6. Take 90kg of Akebia trifoliata, 90kg of Polygonum aviculare, 60kg of Gardenia jasminoides, 80kg of Rheum palmatum, 80kg of Salvia miltiorrhiza, 80kg of Dioscorea hypoglauca, 90kg of Poria cocos and 30kg of Glycyrrhiza uralensis, add 3000kg of 60vol% ethanol aqueous solution, reflux and extract twice, cool to room temperature and filter, combine the filtrates, no alcohol precipitation is required, recover the ethanol until there is no alcohol odor, concentrate under reduced pressure, dry and pulverize to obtain the powder of the eight herbs;
[0040] S7. Take 120 kg of Coptis chinensis, 90 kg of Sophora flavescens and 90 kg of Dianthus superbus, add 1800 kg of 85 vol% ethanol aqueous solution, reflux and extract 3 times, filter, recover ethanol until there is no alcohol taste, concentrate under reduced pressure, dry and pulverize to obtain the powder of the three herbs.
[0041] S8. Take the volatile oil powder, Atractylodes macrocephala and Acorus tatarinowii powder, eight medicinal powders and three medicinal powders and mix them evenly to obtain Lian Shen Tong Lin San (a traditional Chinese medicine formula).
[0042] Add an appropriate amount of excipients (starch is used as the excipient in this example) to Lian Shen Tong Lin powder, mix well, use 85% ethanol as a wetting agent, granulate to obtain Lian Shen Tong Lin granules, labeled as K1.
[0043] Lian Shen Tong Lin Granules can be compressed into tablets or capsules.
[0044] Examples 2-5: Preparation methods of Lian Shen Tong Lin preparations
[0045] Examples 2-5 are preparation methods of a Lian Shen Tong Lin preparation. Their steps are basically the same as those in Example 1, except for the different amounts of raw materials and process parameters. See Table 1 for details.
[0046] Table 1. Summary of process parameters in Examples 2-5
[0047] ,
[0048]
[0049] The contents of other parts of Examples 2 to 5 are the same as those of Example 1, and will not be repeated here.
[0050] The type of adhesive may be at least one of dextrin, polyvinylpyrrolidone K30, polyvinylpyrrolidone (PVP) and sodium carboxymethyl starch, and is not limited to the above embodiments, nor is it limited to the proportions in the above embodiments.
[0051] Comparative Examples 1-13
[0052] Comparative Examples 1-13 are comparative experiments on the preparation process of the Lian Shen Tong Lin preparation in Example 1, using the same batch of Chinese medicinal materials as in Example 1, with other process parameters and dosages remaining unchanged. The only difference is:
[0053] In step S2 of Comparative Example 1, a 10 vol% ethanol aqueous solution was used as the solvent for dynamic extraction, and 1.531 kg of volatile oil powder (equivalent to 0.731 kg of volatile oil) was finally obtained, which was labeled as DG1. The Liancan Tonglin granules prepared were labeled as DK1.
[0054] In step S2 of Comparative Example 2, the temperature for dynamic extraction was 25℃, and 1.625 kg of volatile oil powder (equivalent to 0.825 kg of volatile oil) was finally obtained, labeled as DG2. The Liancan Tonglin granules prepared were labeled as DK2.
[0055] In step S2 of Comparative Example 3, the temperature for dynamic extraction was 90℃, and 1.697 kg of volatile oil powder (equivalent to 0.897 kg of volatile oil) was finally obtained, labeled as DG3. The Liancan Tonglin granules prepared were labeled as DK3.
[0056] In step S3 of Comparative Example 4, the temperature for vacuum concentration was 80°C, and 1.517 kg of volatile oil powder (equivalent to 0.717 kg of volatile oil) was finally obtained, labeled as DG4. The Liancan Tonglin granules prepared from this powder were labeled as DK4.
[0057] The relative density of the concentrate obtained in step S3 of Comparative Example 5 was 1.21, and 1.867 kg of volatile oil powder (equivalent to 1.067 kg of volatile oil) was finally obtained, which was labeled as DG5. The Liancan Tonglin granules prepared were labeled as DK5.
[0058] In step S2 of Comparative Example 6, the amount of β-cyclodextrin used was 0.3 kg, and 0.979 kg of volatile oil powder (equivalent to 0.579 kg of volatile oil) was finally obtained, which was labeled as DG6. The Liancantonglin granules prepared were labeled as DK6.
[0059] The preparation process of the volatile oil powder in Comparative Example 7 is as follows:
[0060] S1. Take 70 kg of Atractylodes macrocephala and 30 kg of Acorus tatarinowii, crush them, and pass them through a 325-mesh sieve to obtain Atractylodes macrocephala and Acorus tatarinowii powder;
[0061] S2. In a dynamic extraction tank, add 100 kg of Atractylodes macrocephala and Acorus tatarinowii powder, add 500 kg of 35 vol% ethanol aqueous solution, and extract dynamically at 60℃ for 2.5 h. After centrifugation and filtration, the resulting extract yields volatile oil liquid and Atractylodes macrocephala and Acorus tatarinowii residue.
[0062] S3. The volatile oil solution is concentrated under reduced pressure to a relative density of 1.06 (i.e., the relative density of the concentrated solution after concentration is 1.06). The temperature of the reduced pressure concentration is 60℃ and the vacuum degree is -0.09MPa. The solution is then passed through a 200-mesh sieve to obtain the volatile oil concentrate.
[0063] S4. Add 0.7 kg of β-cyclodextrin to the concentrated volatile oil solution, and centrifuge emulsify it through a colloid mill at a speed of 3210 r / min and an emulsion fineness of 30 μm. Then add 0.1 kg of dextrin, stir and mix at room temperature, and spray dry to obtain 1.662 kg of volatile oil paste powder (equivalent to 0.862 kg of volatile oil), labeled as DG7.
[0064] Continue to prepare Liancantonglin granules according to the method in Example 1, and label them as DK7.
[0065] In step S4 of Comparative Example 8, the amount of dextrin added was 0.05 kg, and 1.687 kg of volatile oil powder (equivalent to 0.937 kg of volatile oil) was finally obtained, which was labeled as DG8. The Liancantonglin granules prepared were labeled as DK8.
[0066] The preparation process of the volatile oil powder in Comparative Example 9 is as follows:
[0067] S1. Take 70 kg of Atractylodes macrocephala and 30 kg of Acorus tatarinowii, crush them, and pass them through a 325-mesh sieve to obtain Atractylodes macrocephala and Acorus tatarinowii powder;
[0068] S2. Take 100 kg of Atractylodes macrocephala and Acorus tatarinowii powder, and extract the volatile oil by steam distillation until all the oil is extracted, to obtain the volatile oil and the residue of Atractylodes macrocephala and Acorus tatarinowii.
[0069] S3. Take 0.7 kg of β-cyclodextrin and 0.1 kg of dextrin, stir and mix well, then add volatile oil, stir and mix well, then add 65 kg of water, stir again to encapsulate, and then spray dry to obtain 1.565 kg of volatile oil paste powder (equivalent to 0.765 kg of volatile oil), labeled as DG9;
[0070] The Liancantonglin granules were prepared using the method described in Example 1 and labeled as DK9.
[0071] In steps S5 and S6 of Comparative Example 10, 30 vol% ethanol aqueous solution was used as solvent for reflux extraction, and the resulting Liancantonglin granules were labeled as DK10.
[0072] In steps S5 and S6 of Comparative Example 11, 80 vol% ethanol aqueous solution was used as solvent for reflux extraction, and the resulting Liancantonglin granules were labeled as DK11.
[0073] In step S7 of Comparative Example 12, 60 vol% ethanol aqueous solution was used as solvent for reflux extraction, and the resulting Liancantonglin granules were labeled as DK12.
[0074] In step S7 of Comparative Example 13, 95 vol% ethanol aqueous solution was used as solvent for reflux extraction, and the resulting Liancantonglin granules were labeled as DK13.
[0075] As can be seen from Comparative Examples 1 to 9, changing the process parameters or procedures for encapsulating volatile oils will lead to varying degrees of loss of volatile oils; in particular, Comparative Example 9 (i.e., the prior art) shows that extracting volatile oils first and then encapsulating them will obviously lead to a loss of volatile oils, which may be due to problems such as sticking to the walls during the separation of volatile oils.
[0076] Experimental Example 1
[0077] In this embodiment, the following animal experiments were conducted on the Liancan Tonglin granules K1 and DK1~DK13 prepared in Example 1 and Comparative Examples 1~13, respectively.
[0078] I. Effects on diuretic capacity in rats
[0079] Rats weighing 220±20g were placed in metabolic cages and pre-administered with 37℃ warm water via gavage at a dose of 2.5mL / 100g body weight. Animals whose urine output within 2 hours reached more than 40% of the administered volume were eligible for the diuresis test. A total of 160 qualified male and female rats were selected. The 160 rats were randomly divided into 16 groups according to their body weight, with 10 rats in each group. The groups were: blank control group, Example 1 group (administered with Lian Shen Tong Lin Granules K1 prepared in Example 1 via gavage), Comparative Examples 1 to 13 groups (administered with Lian Shen Tong Lin Granules DK1 to DK13 prepared in Comparative Examples 1 to 13 via gavage), and Sanjin Tablets group.
[0080] Blank control group: The same volume of tap water was administered by gavage daily;
[0081] Example 1 and Comparative Examples 1 to 13: 0.60 g / kg of the corresponding granules were administered by gavage (equivalent to 10 times the recommended clinical dose).
[0082] Sanjin tablet group: 0.85g of Sanjin tablet powder / kg administered orally;
[0083] Rats in each experimental group were administered 1.0 mL of the drug via gavage once daily for 14 consecutive days.
[0084] Observation and recording: The urine output of each group was compared, and the results are shown in Table 2.
[0085] Table 2 Summary of the effects on urine output in rats
[0086]
[0087] As shown in Table 2, the urine output of the group in Example 1 of the present invention was significantly higher than that of the blank control group, comparative examples 1 to 13 and the Sanjin tablet group. This shows that the Lianshen Tonglin granules prepared in the present invention can significantly increase the urine output of rats compared with the prior art. In other words, the efficacy of the Lianshen Tonglin granules prepared in the present invention is better than that of the prior art.
[0088] II. Effects of xylene on mouse ear inflammation and edema
[0089] One hundred and sixty Kunming mice, half male and half female, with a weight range of 19±2g, were randomly divided into 16 groups of 10 mice each according to their weight. The groups were: blank control group, Example 1 group (gavaged with Lian Shen Tong Lin granules K1 prepared in Example 1), Comparative Examples 1 to 13 groups (gavaged with Lian Shen Tong Lin granules DK1 to DK13 prepared in Comparative Examples 1 to 13 respectively), and Sanjin tablets group.
[0090] Blank control group: The same volume of tap water was administered by gavage daily;
[0091] Example 1 and Comparative Examples 1 to 13: 0.60 g / kg of the corresponding granules were administered by gavage (equivalent to 10 times the recommended clinical dose).
[0092] Sanjin Tablets Group: 0.85g of Sanjin Tablet powder per kg administered by gavage.
[0093] Each group of drugs was diluted with water to the corresponding concentration and administered by gavage once daily, 0.5 ml each time, for 7 consecutive days. Thirty minutes after administration on the 7th day, 50 μL of xylene was evenly applied to the inner and outer sides of the left ear of each mouse (the right ear was left untreated as a control). One hour later, the mice were euthanized by cervical dislocation, and ear pieces were punched out from the same location on both ears using a 9 mm diameter punch. The weight of the ear pieces was measured. The difference in weight between the two ear pieces was used as the degree of swelling, and the inhibition rate of this invention on xylene-induced ear inflammation and edema in mice was calculated. The results are shown in Table 3.
[0094] Table 3. Summary of the effects of p-xylene on mouse ear inflammation and edema.
[0095]
[0096] The data above show that, compared with the blank control group, comparative examples 1 to 13 and the Sanjin tablet group, the Lian Shen Tong Lin granules K1 in Example 1 of this invention can significantly reduce the swelling of xylene-induced ear membrane inflammation edema in mice and significantly improve the inhibition rate of swelling, further demonstrating that the efficacy of the Lian Shen Tong Lin granules prepared in this invention is superior to the prior art.
[0097] In this experiment, the swelling inhibition rate of Comparative Example 10 was significantly reduced. This may be because the use of 30 vol% ethanol aqueous solution as a solvent in step S5 would cause the sensitizing components to dissolve in the solvent during the extraction process. However, after reflux extraction, no alcohol precipitation was performed, which resulted in the presence of sensitizing components in the prepared Liancan Tonglin Granules DK10. As a result, the prepared Liancan Tonglin Granules DK10, while having an anti-inflammatory effect, also triggered an allergic reaction.
[0098] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.
Claims
1. A method for preparing a Lian Shen Tong Lin preparation, characterized in that, The preparation method of the Liancan Tonglin preparation includes the following steps: Take Atractylodes macrocephala and Acorus tatarinowii powder, add 35~40 vol% ethanol aqueous solution and 0.006~0.008 times the weight of β-cyclodextrin, extract dynamically at 55~60℃ for 2~3 h, filter, and obtain volatile oil liquid and Atractylodes macrocephala and Acorus tatarinowii residue; The volatile oil solution was concentrated under reduced pressure at 55~60℃ and -0.08~-0.10MPa. The relative density of the concentrated solution was 1.05~1.
08. The solution was centrifugally emulsified by a colloid mill, then a binder was added, mixed, and dried to obtain volatile oil powder. Take the residue of Atractylodes macrocephala and Acorus tatarinowii, add 55-60 vol% ethanol aqueous solution, reflux for extraction, cool to room temperature for solid-liquid separation, recover ethanol from the obtained liquid until there is no alcohol odor, concentrate under reduced pressure, dry and pulverize to obtain Atractylodes macrocephala and Acorus tatarinowii powder; the weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to binder is 1:0.0008-0.001; Take eight herbs: Aristolochia debilis, Polygonum aviculare, Gardenia jasminoides, Rheum palmatum, Salvia miltiorrhiza, Dioscorea hypoglauca, Poria cocos, and Glycyrrhiza uralensis. Add 55-60 vol% ethanol aqueous solution, reflux to extract, cool to room temperature and filter, recover ethanol until no alcohol taste, concentrate under reduced pressure, dry and pulverize to obtain the powder of the eight herbs. Take three herbs: Coptis chinensis, Sophora flavescens, and Dianthus superbus. Add 80-90 vol% ethanol aqueous solution, reflux to extract, filter, recover ethanol until no alcohol taste remains, concentrate under reduced pressure, dry, and pulverize to obtain powder of the three herbs. Take volatile oil powder, Atractylodes macrocephala and Acorus tatarinowii powder, eight kinds of medicinal powder and three kinds of medicinal powder and mix them evenly to obtain Lian Shen Tong Lin San (a traditional Chinese medicine formula); The Lian Shen Tong Lin powder is one type of the Lian Shen Tong Lin preparation. The weight ratio of Atractylodes macrocephala, Acorus tatarinowii, Akebia trifoliata, Polygonum aviculare, Gardenia jasminoides, Rheum palmatum, Salvia miltiorrhiza, Dioscorea hypoglauca, Poria cocos, Glycyrrhiza uralensis, Coptis chinensis, Sophora flavescens, and Dianthus superbus is 70:30:90:90:60:80:80:80:90:30:120:90:
90.
2. The preparation method of the Liancan Tonglin preparation according to claim 1, characterized in that, The weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to 35-40 vol% ethanol aqueous solution is 1:4-6.
3. The preparation method of the Liancan Tonglin preparation according to claim 1, characterized in that, The weight ratio of Atractylodes macrocephala and Acorus tatarinowii powder to 55-60 vol% ethanol aqueous solution is 1:5-6; The weight ratio of the eight herbs to a 55-60 vol% ethanol aqueous solution is 1:5-6.
4. The preparation method of the Liancan Tonglin preparation according to claim 1, characterized in that, The weight ratio of the three herbs to an 80-90 vol% ethanol aqueous solution is 1:5-6.
5. The method for preparing the Liancan Tonglin preparation according to claim 1, characterized in that, Add excipients to Lian Shen Tong Lin powder, granulate to obtain Lian Shen Tong Lin granules; Lian Shen Tong Lin Granules can be compressed into tablets or capsules.