A multi-effect plant antibacterial and antiviral preparation

Abstract

The invention provides a multi-effect plant antibacterial and antiviral preparation, which belongs to the technical field of antiviral drug preparation technology. The components of the multi-effect plant antibacterial and antiviral preparation include 0.5-1.8% of emblica extract, 0.5-1.2% of lysozyme, 0.2-0.8% of honeysuckle extract, 0.3-0.8% of scutellaria extract, 0.2-1.2% of egg yolk immunoglobulin, 0.2-0.8% of vitamin C, 3.0-6.0% of xylitol, 1.0-5.0% of glycerol, 0.3-1.0% of poloxamer, and the balance is deionized water. The invention can provide a safe, efficient, good-tasting plant antibacterial and antiviral oral antibacterial liquid which is effective against bacteria and viruses, and is a product which can be used for daily oral health care and auxiliary treatment of oral and upper respiratory tract infections.

Description

Technical Field

[0001] The invention relates to the technical field of oral antibacterial and antiviral pharmaceutical preparations, in particular to a multi-effect plant antibacterial and antiviral preparation. Background Art

[0002] The oral cavity is one of the main ways for bacteria, viruses and other pathogenic microorganisms to enter the human body. Once bacteria, viruses and other pathogenic microorganisms enter the oral cavity, they can cause oral diseases such as caries, gingivitis, and oral ulcers; they may also cause upper respiratory tract infections and digestive tract infections such as pharyngitis, and may also cause systemic diseases; therefore, oral antibacterial and antiviral protection is particularly important.

[0003] At present, oral care products or medicines on the market are mainly bactericidal and anti-inflammatory with single effects. They basically do not have antibacterial and antiviral effects or their antibacterial and antiviral effects are weak.

[0004] Therefore, the present application proposes a multi-effect plant antibacterial and antiviral preparation. Summary of the invention

[0005] In order to overcome the problems in the background technology, the purpose of the present invention is to provide a multi-effect plant antibacterial and antiviral preparation to solve the problem that oral care products or drugs on the market are mainly bactericidal and anti-inflammatory with single effects.

[0006] To achieve the above object, the present invention is implemented through the following technical solutions:

[0007] A multi-effect plant antibacterial and antiviral preparation comprises the following components in percentage by weight: 0.5%-1.8% of emblica extract, 0.5%-1.2% of lysozyme, 0.2%-0.8% of honeysuckle extract, 0.3%-0.8% of scutellaria extract, 0.2%-1.2% of egg yolk immunoglobulin, 0.2%-0.8% of vitamin C, 3.0%-6.0% of xylitol, 1.0%-5.0% of glycerol, 0.3%-1.0% of poloxamer, and the balance is deionized water.

[0008] Furthermore, the multi-effect plant antibacterial and antiviral preparation comprises the following components in weight percentage: 1.0% of emblica extract, 1.0% of lysozyme, 0.5% of honeysuckle extract, 0.5% of scutellaria extract, 0.5% of egg yolk immunoglobulin, 0.5% of vitamin C, 5.0% of xylitol, 3.0% of glycerol, 0.5% of poloxamer, and 87.5% of deionized water.

[0009] The present application also provides a method for preparing a multi-effect plant antibacterial and antiviral preparation, comprising:

[0010] S1: Weigh the extract of Phyllanthus emblica, the extract of Honeysuckle, the extract of Scutellaria baicalensis, and deionized water according to weight percentage, and add them into a liquid preparation tank;

[0011] S2: Heat and stir the liquid preparation tank to 80°C to dissolve the material;

[0012] S3: Filter the fully dissolved material once, with a filtration accuracy of ≤5um;

[0013] S4: After the filtrate is cooled to 50° C., lysozyme, egg yolk immunoglobulin, vitamin C, xylitol, glycerol, and poloxamer are added according to weight percentage and stirred to dissolve;

[0014] S5: The dissolved material is filtered twice with a filtration accuracy of ≤1.5um, and then filled and produced after inspection.

[0015] Beneficial effects of the present invention:

[0016] (1) The Chinese herbal extracts such as Phyllanthus emblica and lysozyme and yolk immunoglobulin in the present invention have a synergistic effect on antibacterial and antiviral effects. The extracts of Phyllanthus emblica are acidic. The preparation can adjust the pH of the oral cavity to form a suitable acidic environment, maintain the stable activity of the components, and has good antibacterial and antiviral dual effects;

[0017] (2) The multi-effect plant antibacterial and antiviral preparation proposed in this application can adhere to the oral cavity after contacting the human mucosa, and has a long-lasting effect, which can inhibit bacteria and viruses for a long time and maintain oral health;

[0018] (3) The multi-effect plant antibacterial and antiviral preparation proposed in the present application is prepared with plant ingredients of honeysuckle, scutellaria baicalensis, and emblica fruit extracts. It has a non-irritating taste and mild effect, and can enhance the user experience of long-term use. DETAILED DESCRIPTION

[0019] In order to make the objectives, technical solutions and beneficial effects of the present invention clearer, the preferred embodiments of the present invention are described in detail below to facilitate understanding by technicians.

[0020] Example 1

[0021] A multi-effect plant antibacterial and antiviral preparation comprising the following components in percentage by weight:

[0022] Emblica officinalis extract 1.0%, lysozyme 1.0%, honeysuckle extract 0.5%, scutellaria baicalensis extract 0.5%, egg yolk immunoglobulin 0.5%, vitamin C 0.5%, xylitol 5.0%, glycerol 3.0%, poloxamer 0.5%, deionized water 87.5%.

[0023] A multi-effect plant antibacterial and antiviral preparation, comprising the following preparation method:

[0024] S1: Weigh 1.0% of Phyllanthus emblica extract, 0.5% of Honeysuckle extract, 0.5% of Scutellaria baicalensis extract, and deionized water according to weight percentage, and add them into a liquid preparation tank;

[0025] S2: Heat and stir the liquid preparation tank to 80°C to dissolve the material;

[0026] S3: Filter the fully dissolved material once with a filtration accuracy of 5um;

[0027] S4: After the filtrate is cooled to 50° C., 1.0% lysozyme, 0.5% egg yolk immunoglobulin, 0.5% vitamin C, 5.0% xylitol, 3.0% glycerol, and 0.5% poloxamer are weighed and added according to weight percentage, and stirred to dissolve;

[0028] S5: The dissolved material is filtered twice with a filtration accuracy of 1.5um, and then filled and produced after inspection.

[0029] Example 2

[0030] A multi-effect plant antibacterial and antiviral preparation comprising the following components in percentage by weight:

[0031] Emblica officinalis extract 0.5%, lysozyme 0.5%, honeysuckle extract 0.2%, scutellaria baicalensis extract 0.3%, egg yolk immunoglobulin 0.2%, vitamin C 0.2%, xylitol 3.0%, glycerol 1.0%, poloxamer 0.3%, and the balance is deionized water.

[0032] A multi-effect plant antibacterial and antiviral preparation, comprising the following preparation method:

[0033] S1: Weigh 0.5% of Phyllanthus emblica extract, 0.2% of Honeysuckle extract, 0.3% of Scutellaria baicalensis extract and deionized water according to weight percentage, and add them into a liquid preparation tank;

[0034] S2: Heat and stir the liquid preparation tank to 80°C to dissolve the material;

[0035] S3: Filter the fully dissolved material once with a filtration accuracy of 5um;

[0036] S4: After the filtrate is cooled to 50°C, 0.5% lysozyme, 0.2% egg yolk immunoglobulin, 0.2% vitamin C, 3.0% xylitol, 1.0% glycerol, and 0.3% poloxamer are weighed and added according to weight percentage, and stirred to dissolve;

[0037] S5: The dissolved material is filtered twice with a filtration accuracy of 1.5um, and then filled and produced after inspection.

[0038] Example 3

[0039] A multi-effect plant antibacterial and antiviral preparation comprising the following components in percentage by weight:

[0040] Emblica officinalis extract 1.8%, lysozyme 1.2%, honeysuckle extract 0.8%, scutellaria baicalensis extract 0.8%, egg yolk immunoglobulin 1.2%, vitamin C 0.8%, xylitol 6.0%, glycerol 5.0%, poloxamer 1.0%, and the balance is deionized water.

[0041] A multi-effect plant antibacterial and antiviral preparation, comprising the following preparation method:

[0042] S1: Weigh 1.8% of Phyllanthus emblica extract, 0.8% of Honeysuckle extract, 0.8% of Scutellaria baicalensis extract, and deionized water according to weight percentage, and add them into a liquid preparation tank;

[0043] S2: Heat and stir the liquid preparation tank to 80°C to dissolve the material;

[0044] S3: Filter the fully dissolved material once with a filtration accuracy of 5um;

[0045] S4: After the filtrate is cooled to 50° C., 1.2% lysozyme, 1.2% egg yolk immunoglobulin, 0.8% vitamin C, 6.0% xylitol, 5.0% glycerol, and 1.0% poloxamer are weighed and added according to weight percentage, and stirred to dissolve;

[0046] S5: The dissolved material is filtered twice with a filtration accuracy of 1.5um, and then filled and produced after inspection.

[0047] Comparative Example 1:

[0048] The difference between Comparative Example 1 and Example 1 is that the weighed amount of the emblica extract is 0.4%.

[0049] Comparative Example 2:

[0050] The difference between Comparative Example 2 and Example 1 is that the weighed amount of the emblica extract is 3%.

[0051] Comparative Example 3:

[0052] The difference between Comparative Example 3 and Example 1 is that the weighed amount of the emblica extract is 4%.

[0053] Experimental Example 1:

[0054] The multi-effect plant antibacterial and antiviral preparation obtained in Example 1 was subjected to an antibacterial ring performance test;

[0055] 1. Equipment

[0056] 1. Test sample: multi-effect plant antibacterial and antiviral preparation;

[0057] 2. Test strains: Escherichia coli (8099), Staphylococcus aureus (ATCC 6538), Candida albicans (ATCC10231), all of which were tested on the 4th generation fresh slant cultures. The above strains were provided by Guangdong Food Microbiological Safety Engineering Technology Research and Development Center.

[0058] 3. Instruments and equipment: KC-SP-YQ-116 vernier caliper, KC-SP-YQ-178 electric constant temperature incubator, KC-SP-YQ-586 biological safety cabinet, KC-SP-YQ-207 pressure steam sterilizer.

[0059] 2. Experimental Methods

[0060] 1. Inspection basis: Technical Specifications for Disinfection (2002 edition) 2.1.8.2.

[0061] 2. Test conditions: temperature 21%~22℃, relative humidity 51%~52%.

[0062] 3. Sample preparation: stock solution.

[0063] 4. Inoculation concentration: 5×10 5 CFU / mL~5×10 6 CFU / mL.

[0064] 3. Experimental Data

[0065] The antibacterial ring data of the multi-effect plant antibacterial and antiviral preparation against Escherichia coli, Staphylococcus aureus, and Candida albicans are shown in Table 1, Table 2, and Table 3 respectively:

[0066] Table 1 Antibacterial ring diameter of sample solution against Escherichia coli

[0067]

[0068] Table 2 Antibacterial ring diameter of sample solution against Staphylococcus aureus

[0069]

[0070] Table 3 Antibacterial ring diameter of sample solution against Candida albicans

[0071]

[0072] 4. Experimental conclusion:

[0073] Under experimental conditions, according to the average inhibition ring diameter data of the multi-effect plant antibacterial and antiviral preparations against Escherichia coli, Staphylococcus aureus, and Candida albicans, the multi-effect plant antibacterial and antiviral preparations had an antibacterial effect.

[0074] Experimental Example 2:

[0075] The multi-effect plant antibacterial and antiviral preparation obtained in Comparative Example 1 was subjected to an antibacterial ring performance test; the test sample was the multi-effect plant antibacterial and antiviral preparation stock solution prepared in Comparative Example 1, and the experimental equipment and experimental methods were the same as those in Experimental Example 1.

[0076] Experimental data

[0077] The antibacterial ring data of the multi-effect plant antibacterial and antiviral preparation against Escherichia coli, Staphylococcus aureus, and Candida albicans are shown in Table 4, Table 5, and Table 6 respectively:

[0078] Table 4 Antibacterial ring diameter of sample solution against Escherichia coli

[0079]

[0080] Table 5 Antibacterial ring diameter of sample solution against Staphylococcus aureus

[0081]

[0082] Table 6 Antibacterial ring diameter of sample solution against Candida albicans

[0083]

[0084] Experimental Example 3:

[0085] The multi-effect plant antibacterial and antiviral preparation obtained in Comparative Example 2 was subjected to an antibacterial ring performance test; the test sample was the multi-effect plant antibacterial and antiviral preparation stock solution prepared in Comparative Example 2, and the experimental equipment and experimental methods were the same as those in Experimental Example 1.

[0086] Experimental data

[0087] The antibacterial ring data of the multi-effect plant antibacterial and antiviral preparation against Escherichia coli, Staphylococcus aureus, and Candida albicans are shown in Table 7, Table 8, and Table 9, respectively:

[0088] Table 7 Antibacterial ring diameter of sample solution against Escherichia coli

[0089]

[0090] Table 8 Antibacterial ring diameter of sample solution against Staphylococcus aureus

[0091]

[0092] Table 9 Antibacterial ring diameter of sample stock solution against Candida albicans

[0093]

[0094] Experimental Example 4:

[0095] The multi-effect plant antibacterial and antiviral preparation obtained in Comparative Example 3 was subjected to an antibacterial ring performance test; the test sample was the stock solution of the multi-effect plant antibacterial and antiviral preparation prepared in Comparative Example 3, and the experimental equipment and experimental methods were the same as those in Experimental Example 1.

[0096] Experimental data

[0097] The antibacterial ring data of the multi-effect plant antibacterial and antiviral preparation against Escherichia coli, Staphylococcus aureus, and Candida albicans are shown in Table 10, Table 11, and Table 12, respectively:

[0098] Table 10 Antibacterial ring diameter of sample solution against Escherichia coli

[0099]

[0100] Table 11 Antibacterial ring diameter of sample solution against Staphylococcus aureus

[0101]

[0102] Table 12 Antibacterial ring diameter of sample solution against Candida albicans

[0103]

[0104] Conclusion: The samples prepared in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 of the present invention all have antibacterial effects, and the antibacterial and antiviral effects of Experimental Example 1 are the best;

[0105] Compared with Example 1, Comparative Example 1 has a low content of Phyllanthus emblica extract and has little effect on the pH of the oral environment. Lysozyme and yolk immunoglobulin fail to achieve optimal stable activity within the pH of the oral environment, and their retention in the oral cavity and antibacterial and antiviral effects are not as good as those in Example 1.

[0106] Compared with Example 1, Comparative Examples 2 and 3 have high content of Phyllanthus emblica extract, which has a great impact on the pH of the oral environment, making the pH of the oral environment acidic, and lysozyme and yolk immunoglobulin fail to achieve optimal stable activity, and the antibacterial and antiviral effects are not as good as those of Example 1.

[0107] Experimental Example 5

[0108] Testing the virus killing effect of multi-effect plant antibacterial and antiviral preparations

[0109] 1. Test equipment

[0110] 1. Experimental virus strain: poliovirus type 1 vaccine strain;

[0111] 2. Host name: Ve58 cells;

[0112] 3. Equipment: cell culture flask, 96-well culture plate, constant temperature water bath, carbon dioxide incubator, laminar flow clean bench, low-temperature refrigerator (-20℃, -80℃), liquid nitrogen tank, inverted microscope, centrifuge, adjustable pipette and matching disposable plastic tips.

[0113] 2. Test method: "Disinfection Technical Specifications" 2002 Edition - 2.1.1.10.7 Poliovirus Inactivation Test

[0114] 3. Test samples: Samples of Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 correspond to test group 1, test group 2, test group 3 and test group 4, respectively; deionized water is used as control group 1, control group 2, control group 3 and control group 4.

[0115] 4. Experimental Principle

[0116] 1. Use the cell infection method to measure the amount of poliomyelitis in the samples before and after the sample is exposed (or between the experimental group and the control group), use the cell lesion as the judgment index, determine the infection titer of the virus in each group, and calculate the inactivation rate of poliomyelitis by the disinfectant;

[0117] 2. The test group estimates the killing or inactivation dose of the tested sample on other microorganisms and sets the appropriate concentration and action time group;

[0118] 3. The control group used deionized water instead of samples, and added poliomyelitis suspension for testing and culture according to the prescribed steps of the experimental group to observe whether the poliomyelitis grows well. At the same time, a complete culture medium without poliomyelitis was used as a negative control to observe whether the culture medium used was contaminated and whether the cells grew well.

[0119] 5. Test data: as shown in Table 13

[0120] Table 13 Virus inactivation test of sample stock solution

[0121]

[0122] Conclusion: The samples of Example 1, Comparative Examples 1, 2 and 3 of the present invention all have good antibacterial and antiviral effects. There is no obvious difference in the virus killing effects of the four groups of samples, and the antibacterial effect of the sample of Example 1 is the best.

[0123] Animal Experimentation:

[0124] The multi-effect plant antibacterial and antiviral preparation prepared in Example 1 was subjected to an acute eye irritation test;

[0125] 1. Experimental Materials and Animals

[0126] 1. Test samples

[0127] Name: Multi-effect plant antibacterial and antiviral preparation, Properties: Liquid

[0128] 2. Animals

[0129] Name: New Zealand Rabbit

[0130] Level: General

[0131] Number and gender: 3, male

[0132] Weight: 2.0kg~2.5kg

[0133] Breeding unit: Jinan Xilingjiao Breeding and Breeding Center

[0134] Production license number: SCXK (Lu) 2020 0004

[0135] Quality certificate number: No.370822211100008622

[0136] 3. Rearing environment

[0137] Temperature: 16℃~26℃

[0138] Relative humidity: 40%-70%

[0139] 4. Feed

[0140] Name: Rabbit Feed

[0141] Manufacturer: Jinan Xilingjiao Breeding and Breeding Center

[0142] Production license number: SCXK (Lu) 2018 0010

[0143] Quality certificate number: No.3708242100002451

[0144] 2. Methods

[0145] 1. Inspection basis: Technical Specifications for Disinfection (2002 edition) 2.3.4 Acute eye irritation test.

[0146] 2. Sample preparation: Multi-effect plant antibacterial and antiviral preparation solution.

[0147] 3. Test methods:

[0148] 3.1 Preparation of experimental animals: New Zealand rabbits were pre-raised in the animal room of this laboratory for 3 days to adapt to the environment. Within 24 hours before the experiment, both eyes of each New Zealand rabbit were examined and no abnormalities were found, so they could be used for the experiment.

[0149] 3.2 Poisoning method: Use the left and right side self-comparison method, take 0.1mL of the test substance solution and drop it into the conjunctival sac of the left eye, close the eye passively for 4s, and rinse with sodium chloride injection after about 30s. The right eye is given 0.1mL of sodium chloride injection in the same way as the negative control group.

[0150] 3.3 Observe the damage and recovery of the conjunctiva, iris and cornea of ​​the New Zealand rabbits with naked eyes at 1h, 24h, 48h and 72h after eye drops and record them. If no irritation reaction occurs within 72h, the test is terminated. Score the acute irritation reaction of the cornea, iris and conjunctiva of the New Zealand rabbits according to the scoring criteria for acute eye irritation reaction of rabbits in Table 2-13 in 2.3.4 of the Technical Specifications for Disinfection (2002 Edition), and calculate the "average score" of corneal damage, iris damage, conjunctival congestion and conjunctival edema for each animal at three different observation times (24h, 48h and 72h) (i.e. the sum of the scores of each animal at 24h, 48h and 72h divided by the number of observations 3). The average scores and recovery time of corneal, iris and conjunctival congestion and edema of the animals are used to determine the irritation intensity of the test substance to the eyes according to the eye irritation reaction grading standards in Table 2-14 and Table 2-15 in 2.3.4 of the "Technical Specifications for Disinfection" (2002 edition).

[0151] 3. Experimental Results

[0152] The "average scores" of corneal damage, iris damage, conjunctival congestion and conjunctival edema were measured 24h, 48h and 72h after the removal of the test substance. Under the experimental conditions, the type of damage caused by the multi-effect plant antibacterial and antiviral preparation to New Zealand rabbit eyes was non-irritating, as shown in Table 14.

[0153] Table 14 New Zealand rabbit eye irritation response score

[0154]

[0155] Note: The average score refers to the sum of the 24h, 48h, and 72h scores divided by the number of observation time periods 3

[0156] Heavy Metal Detection:

[0157] The multi-effect plant antibacterial and antiviral preparation prepared in Example 1 was tested for heavy metals (mercury, lead, arsenic);

[0158] 1. Testing equipment

[0159] 1. Test sample: Multi-effect plant antibacterial and antiviral preparation concentrate.

[0160] 2. Instruments and equipment: KC-SP-YQ-209 inductively coupled plasma mass spectrometer (iCAP RQ), KC-SP-YQ-051 electronic balance (AE224C).

[0161] 3. Reagent name and grade: Nitric acid UP grade, 30% hydrogen peroxide ultra-pure, hydrochloric acid ultra-pure.

[0162] 4. The concentrations of lead and arsenic standard solutions are both 100 μg / mL, and the concentration of mercury standard solution is 1000 μg / mL.

[0163] 2. Methods

[0164] 1. Inspection basis: "Technical Specifications for Safety of Cosmetics" (2015 edition), Chapter 4, 1.6.

[0165] 2. Test conditions: temperature 19°C, relative humidity 40%.

[0166] 3. Sample processing: stock solution.

[0167] 3. Results

[0168] The lead and mercury in the original solution of the multi-effect plant antibacterial and antiviral preparation were not detected, and the arsenic content was <0.0033 mg / kg (minimum quantitative concentration). The results are shown in Table 15.

[0169] Table 15 Results of lead, mercury and arsenic determination

[0170]

[0171] Microbiological index detection: The multi-effect plant antibacterial and antiviral preparation prepared in Example 1 was subjected to microbiological index detection;

[0172] 1. Equipment

[0173] 1. Test sample: Multi-effect plant antibacterial and antiviral preparation concentrate.

[0174] 2. Instruments and equipment: KC-SP-YQ-594 clean workbench, KC-SP-YQ-180 electric constant temperature incubator, KC-SPYQ-082 mold incubator.

[0175] 2. Methods

[0176] 1. Inspection basis: Appendix B of GB 15979-2002 Hygiene Standard for Disposable Sanitary Products.

[0177] 2. Test conditions: temperature 21°C, relative humidity 52%.

[0178] 3. Sample processing: stock solution.

[0179] 3. Results

[0180] The total bacterial colony count and fungal colony count of the multi-effect plant antibacterial and antiviral preparation stock solution were both <1 CFU / mL.

[0181] Coliform bacteria, Staphylococcus aureus, Pseudomonas aeruginosa, and hemolytic streptococci were not detected. The results are shown in Table 16.

[0182] Table 16 Microbiological index determination results

[0183]

Claims

1. A multi-effect plant antibacterial and antiviral preparation, characterized in that: Contains the following components in weight percentage: Emblica extract 0.5%-1.8%; Lysozyme 0.5%-1.2%; Honeysuckle extract 0.2%-0.8%; Scutellaria baicalensis extract 0.3%-0.8%; Yolk immunoglobulin 0.2%-1.2%; Vitamin C 0.2%-0.8%; Xylitol 3.0%-6.0%; Glycerin 1.0%-5.0%; Poloxamer 0.3%-1.0%; The balance was deionized water.

2. The multi-effect plant antibacterial and antiviral preparation according to claim 1, characterized in that: Contains the following components in weight percentage: Emblica officinalis extract 1.0%; Lysozyme 1.0%; Honeysuckle extract 0.5%; Scutellaria baicalensis extract 0.5%; Yolk immunoglobulin 0.5%; Vitamin C 0.5%; Xylitol 5.0%; Glycerol 3.0%; Poloxamer 0.5%; Deionized water 87.5%.

3. The method for preparing the multi-effect plant antibacterial and antiviral preparation according to claim 1, characterized in that: S1: Weigh the emblica extract, honeysuckle extract, scutellaria extract and deionized water according to weight percentage, and add them into a liquid preparation tank; S2: Heat and stir the liquid preparation tank to 80°C to dissolve the material; S3: Filter the fully dissolved material once, with a filtration accuracy of ≤5um; S4: After the filtrate is cooled to 50° C., lysozyme, egg yolk immunoglobulin, vitamin C, xylitol, glycerol, and poloxamer are weighed and added according to weight percentage, and stirred to dissolve; S5: The dissolved material is filtered twice with a filtration accuracy of ≤1.5um, and then filled and produced after inspection.

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