Application of ferulic acid in promoting the transformation of muscle fiber types in Mongolian horses

Abstract

The present invention provides the application of ferulic acid in promoting the transformation of Mongolian horse muscle fiber types. It belongs to the technical field of feed additives. The present invention discloses for the first time the effect of ferulic acid on the transformation of Mongolian horse skeletal muscle fiber types, and applies it in the culture process of Mongolian horse myofibroblasts. The study found that after adding ferulic acid, at the gene level, ferulic acid can significantly inhibit the expression of slow muscle genes and promote the expression of fast muscle genes, proving that ferulic acid has the effect of promoting the transformation of Mongolian horse skeletal muscle fibers from slow muscles to fast muscles, filling the gap in horse feed on muscle fiber type transformation.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and more specifically to the application of ferulic acid in promoting the transformation of Mongolian horse muscle fiber types. Background Art

[0002] The Mongolian horse is one of the oldest horse breeds in the world and is known for its excellent endurance performance. Horse racing is very popular in the field of international horse sports. In competitive equestrianism and horse racing, the speed and endurance of horses are key competitive factors. By studying how to enhance the speed or endurance of horses, the competitiveness of horses in competitions can be improved. One of the determining factors of running speed and endurance is the proportion of skeletal muscle types. When the proportion of fast muscles is higher, the speed is faster, and when the proportion of slow muscles is higher, the endurance is stronger. However, the proportion of skeletal muscle fiber types is not static. Various factors can cause the skeletal muscle fiber types to transform, thereby adjusting the endurance and speed of horses. The proportion of muscle fiber types in animals not only affects the weight gain of animals, but also has a certain impact on the meat quality of livestock, the movement speed and endurance of animals.

[0003] In recent years, more and more countries have begun to ban the use of antibiotics, and more and more natural additives have come into everyone's sight. Most additives that promote fast muscle growth are hormones or drugs, which are excitatory to animals and have serious side effects.

[0004] Therefore, how to find a natural additive that promotes the transformation of Mongolian horse muscle fibers is a technical problem that technicians in this field urgently need to solve. Summary of the invention

[0005] In view of this, the present invention proposes the use of ferulic acid in promoting the transformation of Mongolian horse muscle fiber types. It is the first time that ferulic acid is used in Mongolian horses, which effectively promotes the transformation of Mongolian horse skeletal muscle fiber types and improves the running speed and endurance of Mongolian horses.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] Application of ferulic acid in promoting muscle fiber type transformation in Mongolian horses.

[0008] Another object of the present invention is to provide: use of ferulic acid in the preparation of additives and or feeds for promoting the transformation of Mongolian horse muscle fiber types.

[0009] Another object of the present invention is to provide: a product for promoting the transformation of Mongolian horse muscle fiber types, wherein the product comprises ferulic acid.

[0010] Preferably, the product also includes feed and / or additives, but is not limited to feed and / or additives.

[0011] Another object of the present invention is to provide: a method for promoting the transformation of Mongolian horse muscle fiber types, by feeding ferulic acid during the Mongolian horse breeding process.

[0012] It can be seen from the above technical solution that compared with the prior art, the present invention has the following beneficial effects:

[0013] (1) The present invention discloses for the first time the effect of ferulic acid on the transformation of Mongolian horse skeletal muscle fiber types, and applies it to the culture process of Mongolian horse myofibroblasts. The study found that after adding ferulic acid, ferulic acid inhibited the expression of slow muscle genes and promoted the expression of fast muscle genes, proving that ferulic acid can promote the transformation of Mongolian horse skeletal muscle fibers from slow muscle to fast muscle, thereby improving the running speed and endurance of Mongolian horses.

[0014] (2) Ferulic acid, as an additive, is non-excitatory and highly palatable. It can replace traditional antibiotics as a healthier, non-toxic and harmless natural additive, allowing herders to adjust the proportion of skeletal muscle fiber types in horses in the most convenient and quickest way, filling the gap in the conversion of muscle fiber types in horse feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0016] Figure 1 The following are: microscopic pictures of Example 1;

[0017] Figure 2 The CCK8 method in Example 1 was used to test the effects of different concentrations of ferulic acid on myoblast proliferation;

[0018] Figure 3 In Example 2, the expression of fast-twitch and slow-twitch muscle fiber marker genes was observed using immunofluorescence method. DETAILED DESCRIPTION

[0019] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] Example 1

[0021] In vitro culture of Mongolian horse myoblasts

[0022] The skeletal muscle of Mongolian horses was collected and the blood was washed with physiological saline. The tissue was cut into minced meat, 0.25% EDTA was added, and the enzymatic digestion method was used to digest the skeletal muscle until the tissue was no longer visible. The skeletal muscle was filtered using a 100um cell filter, and the filtered liquid was centrifuged at 1500rpm for 5 minutes to obtain a cell precipitate. The culture medium was added and mixed and inoculated into a culture dish for culture. The culture was taken, fixed with 4% paraformaldehyde for 15 minutes, blocked with BSA liquid for 1 hour, and washed with PSB; incubated with myoblast-specific protein Desmin antibody, washed with PBS; incubated with DAPI for 15 minutes, washed with PBS; incubated with the same species secondary antibody for 1 hour, washed with PBS, and sealed, photographed under a microscope, and tested with myoblast-specific protein Desmin to prove that the Mongolian horse myoblast culture was successful ( Figure 1 ).

[0023] Different concentrations of ferulic acid were added to myoblasts, and the CCK8 method was used to test the effects of different concentrations of ferulic acid on myoblast proliferation and determine the optimal concentration of ferulic acid. After CCK8 detection, it was found that the best effect on myoblasts was achieved when 500 ng / mL ferulic acid was added ( Figure 2 ).

[0024] Example 2

[0025] Verification of the effect of ferulic acid on the transformation of skeletal muscle fiber types in Mongolian horses

[0026] Immunofluorescence was used to observe the expression of fast-twitch fiber marker genes PGK1, ACTN3, TNNI2, and SOX6 and slow-twitch fiber marker genes TNNT1, ATP2A2, and MYH7B, and to preliminarily verify the effect of ferulic acid on myoblast fiber type. Figure 3 ).

[0027] Subsequent studies found that ferulic acid affects the TCA cycle by binding to PDK1 (pyruvate dehydrogenase kinase 1), thereby promoting the conversion of slow muscles to fast muscles in Mongolian horses.

[0028] Result analysis: Ferulic acid can significantly reduce the expression of slow muscle genes and significantly increase the expression of fast muscle genes, which further proves that ferulic acid can promote the transformation of Mongolian horse slow muscle to fast muscle.

[0029] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0030] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Application of ferulic acid in promoting the transformation of Mongolian horse muscle fibers from slow twitch to fast twitch for the purpose of non-disease diagnosis and treatment.

2. Application of ferulic acid in the preparation of additives and / or feeds for promoting the transformation of Mongolian horse muscle fibers from slow muscles to fast muscles.

3. A method for promoting the transformation of Mongolian horse muscle fibers from slow muscle to fast muscle for the purpose of non-disease diagnosis and treatment, characterized in that: Ferulic acid was fed to Mongolian horses during their breeding process.

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