A microbial composite fertilizer and fermentation medium thereof

Abstract

The present invention relates to a microbial composite fertilizer and a fermentation medium thereof, and belongs to the field of microbial technology. The microbial composite fertilizer of the present invention comprises 8-10g of a microbial agent and 40-60g of a non-microbial raw material. The microbial agent is produced from a fermentation medium containing fermented bone residue powder, mulberry linterinary polypeptide, broken yeast powder, carotene, wheat bran powder, chicory polysaccharide, calcium carbonate, and dipotassium hydrogen phosphate. The microbial fertilizer of the present invention adopts an effective combination of microbial agents and inorganic fertilizers, which can fix nitrogen in the air in the soil through nitrogen-fixing organisms, activate the insoluble phosphorus and potassium elements in the soil into a state that can be absorbed and utilized by plants, and improve the physical and chemical properties of the soil to a certain extent; at the same time, the use of the fertilizer can improve the physical properties of the soil, improve the aggregate structure in the soil, increase the voids in the soil, and improve the permeability and water permeability of the soil.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms and relates to a microbial composite fertilizer and a fermentation culture medium thereof. Background Art

[0002] Soil compaction refers to the phenomenon in which the soil surface, lacking organic matter and having a poor structure, is damaged and dispersed by external factors such as irrigation or rainfall. After drying, the soil surface becomes hardened due to cohesive forces. It is an important indicator of soil fertility. Soil aggregate structure is a key indicator of soil fertility. Destruction of the soil aggregate structure reduces the soil's ability to retain water and fertilizer, as well as its permeability, resulting in soil compaction. When soil is compacted, a lack of oxygen can reduce the vitality of agricultural product roots, preventing them from developing normally. This weakens root cell respiration, and nutrients such as nitrogen, which are mostly present in an ionic state, consume energy generated by cellular metabolism during absorption. This weakened respiration leads to insufficient energy supply, impacting nutrient absorption. This reduced root absorption capacity can lead to nutrient deficiencies in agricultural products.

[0003] The main reasons for soil compaction are: 1. The long-term use of traditional irrigation methods such as flooding or hose irrigation has destroyed the soil's aggregate structure and squeezed out the air in the soil. 2. The serious lack of organic fertilizer and the reduced amount of straw returned to the field have resulted in insufficient replenishment of organic matter in the soil. The reduced amount of straw returned to the field has caused the soil's organic matter content to be low and the structure to deteriorate, affecting the activity of microorganisms, thereby affecting the formation of soil aggregate structure, causing the soil's acidity or alkalinity to be too high or too low, leading to soil compaction. 3. The long-term single application of chemical fertilizers has resulted in a serious lack of farmyard manure, a decrease in soil organic matter, and the inability to replenish humus in a timely manner, causing soil compaction and cracking: (1) Excessive application of nitrogen fertilizer: For every 1 part of nitrogen supply to microorganisms, the corresponding carbon consumption increases by 25 parts. The consumed carbon comes from soil organic matter. The low organic matter content affects the activity of microorganisms, thereby affecting the formation of soil aggregate structure and leading to soil compaction. (2) Excessive application of phosphate fertilizer: The phosphate ions in phosphate fertilizer combine with cations such as calcium and magnesium in the soil to form insoluble phosphates, which not only wastes phosphate fertilizer but also destroys the soil aggregate structure, causing soil compaction. (3) Excessive application of potassium fertilizer: The potassium ions in potassium fertilizer have a particularly strong substitution ability and can replace the multivalent cations that form the soil aggregate structure. However, the monovalent potassium ions do not have a bridging effect. The bond bridges of the soil aggregate structure are destroyed, thus destroying the aggregate structure and causing soil compaction.

[0004] Microbial fertilizers are particularly effective in improving soil fertility and the quality of agricultural products. Once applied to the soil, they rapidly multiply and form a vibrant community of beneficial bacteria, fundamentally improving the ecological structure of the soil and the surrounding root systems. This loosens and allows for the absorption of nitrogen from the air, creating an "ecological nitrogen fertilizer," which activates ineffective, dead phosphorus and potassium deposited in the soil for absorption by plant roots. The beneficial microorganisms in microbial fertilizers produce sugars that, combined with plant mucus, mineral embryos, and organic colloids, improve soil aggregate structure, effectively breaking down soil compaction and promoting aggregate formation. They also improve soil aeration and promote the production of organic matter, humic acid, and humus.

[0005] Although microbial fertilizers have a long history of application in agricultural production, there are still many unresolved problems in their practical application. Although many microbial fertilizers show good results in the early stages of research and development, they often become unstable or lose their effectiveness when used on a large scale in the field. The main reasons are: (1) During the reproduction and subculture process, the strains' potassium, phosphorus, and nitrogen fixation properties weaken or even disappear. (2) Faced with complex soil and climatic environments, microorganisms have weak coping capabilities and cannot colonize and propagate well on the roots of crops. (3) The effective period of live bacteria is short, and the number of effective bacteria gradually decreases as the storage time increases. Summary of the Invention

[0006] The main purpose of the present invention is to provide a new type of microbial composite fertilizer, which can effectively improve soil compaction and improve soil fertilizer efficiency. At the same time, a fermentation culture medium for producing the microbial composite fertilizer is provided. The culture medium can obtain a highly active functional microbial flora with strong adaptability, can quickly adapt to and activate soils in different environments, enhance soil organic matter content, form a granular structure, and improve soil permeability and water and fertilizer retention properties.

[0007] The present invention adopts the following technical solutions to achieve the above-mentioned purpose:

[0008] A microbial agent contains 330-350 million cfu / g of Bacillus gelatinosa, 450-500 million cfu / g of Bacillus circulans, 150-200 million cfu / g of Bacillus megaterium, 80-120 million cfu / g of Penicillium, and 50-80 million cfu / g of Streptomyces roseo.

[0009] The present invention provides a fermentation culture medium for producing the above-mentioned microbial agent. The fermentation culture medium contains the following components: 15-18 g / L of fermented bone residue powder, 8-10 g / L of mulberry ignia polypeptide, 20-25 g / L of cracked yeast powder, 3-5 g / L of carotene, 15-20 g / L of wheat bran powder, 5-8 g / L of chicory polysaccharide, 3-6 g / L of calcium carbonate, and 0.5-0.8 g / L of dipotassium hydrogen phosphate, and the pH is adjusted to 6.5-7.5.

[0010] The present invention provides a method for preparing the fermented bone residue powder in the fermentation medium, which specifically comprises the following steps:

[0011] The waste bones are crushed and added with water of equal weight to prepare a suspension, 0.5-0.8% of the weight of the bones by Saccharomyces boulardii and 0.1-0.12% of acetic acid bacteria are added, and the suspension is fermented at 30-35° C. for 1-1.5 days. After the fermentation is completed, the suspension is sterilized at high temperature and the fermentation product is dried to obtain the fermented bone residue powder.

[0012] Furthermore, in the fermented bone residue powder preparation method, the activity of Saccharomyces boulardii is 20 billion cfu / g, and the activity of acetic acid bacteria is 3 billion cfu / g.

[0013] The present invention provides a method for preparing the above-mentioned microbial agent, which comprises the following steps:

[0014] Step A: activating Bacillus jelly, Bacillus circulans, Bacillus megaterium, Penicillium, and Streptomyces roseus, respectively, and culturing them in a seed culture medium until the OD600 reaches 0.4-0.6 to obtain a seed solution;

[0015] Step B: inoculating the seed liquid into the above fermentation medium respectively, fermenting for 1-1.5 days, and freeze-drying and crushing the fermentation product to obtain the microbial agent.

[0016] Furthermore, the inoculation amount of the gelatinous Bacillus seed solution is 3-5 mL / L, the inoculation amount of the circulans Bacillus seed solution is 4-8 mL / L, the inoculation amount of the megaterium seed solution is 1.5-3 mL / L, the inoculation amount of the Penicillium seed solution is 0.5-0.8 mL / L, and the inoculation amount of the rose-colored Streptomyces seed solution is 0.5-1 mL / L.

[0017] The invention provides a microbial composite fertilizer, which contains 8-10g of the above-mentioned microbial agent and 40-60g of non-microbial raw materials in grams; wherein the non-microbial raw materials contain 10-15g of sodium alginate, 15-20g of polyethylene glycol, 10-15g of bagasse powder and 5-10g of attapulgite.

[0018] The present invention provides a method for preparing the above-mentioned microbial composite fertilizer, which comprises the following steps:

[0019] Step 1: adding molten sodium alginate to polyethylene glycol, mixing and drying to obtain a mixture for later use;

[0020] Step 2: Add water to the mixture obtained in step 1 and stir until it is in a gel state, then add the microbial raw materials, mix evenly, dry, add bagasse powder and attapulgite, and mix evenly to obtain the microbial compound fertilizer.

[0021] The present invention has the following beneficial effects:

[0022] 1. The microbial fertilizer of the present invention adopts an effective combination of microbial agents and inorganic fertilizers, which can fix nitrogen in the air in the soil through nitrogen-fixing organisms, activate the insoluble phosphorus and potassium elements in the soil into a state that can be absorbed and utilized by plants, and improve the physical and chemical properties of the soil to a certain extent; at the same time, the use of the microbial fertilizer can improve the physical properties of the soil, improve the aggregate structure in the soil, increase the voids in the soil, improve the permeability and water permeability of the soil, and at the same time increase the nutrients in the soil, increase trace elements, improve the nutrient structure of the soil, and improve crop yield and quality.

[0023] 2. The microbial fertilizer of the present invention has high microbial activity and strong biological stability, which can effectively improve the soil aggregate structure, eliminate soil compaction, enhance soil permeability, improve soil buffering performance, and alleviate the harm to crops caused by secondary salinization of the soil due to excessive application of chemical fertilizers.

[0024] 3. The microbial fertilizer of the present invention can promote the growth and development of beneficial microorganisms in the soil, improve the utilization rate of fertilizers, promote the growth of crop roots, develop the root system, enhance the disease resistance of crops, and have a significant yield-increasing effect.

[0025] 4. In the preparation of the microbial fertilizer of the present invention, a culture medium containing multiple ingredients such as fermented bone residue powder, mulberry linterinary polypeptide, and broken yeast powder is used to ferment and produce a microbial agent. The culture medium is rich in nutrients and can provide necessary nutrients for the growth and reproduction of microorganisms. During the fermentation production process, the acid-base (pH) balance of the fermentation environment can be maintained, bacterial growth and reproduction can be promoted, the spore formation rate can be increased, and the fermentation cycle can be shortened, so that a dominant bacterial population can be formed in a short time. Secondly, in the production process of the microbial fertilizer, the salt tolerance, acid and alkali tolerance, cold and heat tolerance, and drug resistance of the microorganisms can be improved by using sodium alginate embedding. Adding polyethylene glycol to the sodium alginate embedding material can solve the weakness of sodium alginate having low strength. DETAILED DESCRIPTION

[0026] The present invention is further illustrated below with reference to specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention made by those skilled in the art all fall within the scope of protection of the claims of this application.

[0027] Example 1 Microbial fertilizer

[0028] 1. Preparation of microbial agents

[0029] Preparation of fermentation medium: First, 1 kg of discarded bones was crushed and an equal weight of water was added to form a suspension, 5 mg of Saccharomyces boulardii (commercially available with an activity of 20 billion cfu / g of bacterial powder) and 1.2 mg of acetic acid bacteria (commercially available with an activity of 3 billion cfu / g of bacterial powder) were added, and fermented at 35°C for 1 day. After the fermentation was completed, the mixture was sterilized at high temperature and the fermentation product was dried to obtain fermented bone residue powder; then, a fermentation medium was prepared according to the ratio of 15 g / L of fermented bone residue powder, 10 g / L of mulberry linterinary polypeptide, 20 g / L of broken yeast powder, 5 g / L of carotene, 15 g / L of wheat bran powder, 5 g / L of chicory polysaccharide, 6 g / L of calcium carbonate, and 0.8 g / L of dipotassium hydrogen phosphate, and the pH was adjusted to 7.5;

[0030] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.4; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.52; Bacillus megaterium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The seed solution was 0.46; Penicillium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The seed solution was 0.57; the Streptomyces roseus strain was activated according to the strain instructions and placed in the seed culture medium to culture until the OD 600 is 0.43, and the seed solution is obtained;

[0031] Production: The above seed liquid is inoculated into the above-obtained fermentation medium according to the inoculum amount of 3 mL / L of Bacillus gelatinosa, 8 mL / L of Bacillus circulans, 1.5 mL / L of Bacillus megaterium, 0.8 mL / L of Penicillium, and 0.5 mL / L of Streptomyces roseus, respectively. Ferment for 1 day. The fermentation product is freeze-dried and then crushed to obtain the microbial agent.

[0032] The obtained microbial agent was tested and found to contain 3.02 billion cfu / g of Bacillus gelatinosa, 4.86 billion cfu / g of Bacillus circulans, 1.34 billion cfu / g of Bacillus megaterium, 830 million cfu / g of Penicillium, and 310 million cfu / g of Streptomyces roseo.

[0033] 2. Preparation of microbial fertilizer

[0034] First, 15 g of sodium alginate was heated to a molten state, and then added to 20 g of polyethylene glycol, mixed, and dried to obtain a mixture for later use;

[0035] Secondly, add water to the mixture obtained above and stir until it is in a gel state, then add 10g of the above-mentioned microbial agent, mix evenly, dry, add 15g of sugarcane bagasse powder and 5g of attapulgite, mix evenly, and obtain microbial fertilizer.

[0036] Example 2 Microbial Fertilizer

[0037] 1. Preparation of microbial agents

[0038] Preparation of fermentation medium: First, 1 kg of discarded bones was crushed and an equal weight of water was added to form a suspension, 8 mg of Saccharomyces boulardii (commercially available with an activity of 20 billion cfu / g of bacterial powder) and 1 mg of acetic acid bacteria (commercially available with an activity of 3 billion cfu / g of bacterial powder) were added, and fermented at 30°C for 1.5 days. After the fermentation was completed, the mixture was sterilized at high temperature and the fermentation product was dried to obtain fermented bone residue powder; then, a fermentation medium was prepared according to the ratio of 18 g / L of fermented bone residue powder, 8 g / L of mulberry linterinary polypeptide, 25 g / L of broken yeast powder, 3 g / L of carotene, 20 g / L of wheat bran powder, 8 g / L of chicory polysaccharide, 3 g / L of calcium carbonate, and 0.5 g / L of potassium dihydrogen phosphate, and the pH was adjusted to 6.5;

[0039] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.43; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.51; after Bacillus megaterium was activated according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.56; Penicillium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The Streptomyces roseus strain was activated according to the strain instructions and placed in the seed culture medium to culture until the OD 600 is 0.49, and the seed solution is obtained;

[0040] Production: The above seed liquid is inoculated into the above-obtained fermentation medium according to the inoculum amount of 5 mL / L of Bacillus gelatinosa, 4 mL / L of Bacillus circulans, 3 mL / L of Bacillus megaterium, 0.5 mL / L of Penicillium, and 1 mL / L of Streptomyces roseus, respectively. The fermentation is carried out for 1.5 days. The fermentation product is freeze-dried and then crushed to obtain the microbial agent.

[0041] The obtained microbial agent was tested and found to contain 4.47 billion cfu / g of Bacillus gelatinosa, 4.38 billion cfu / g of Bacillus circulans, 1.02 billion cfu / g of Bacillus megaterium, 970 million cfu / g of Penicillium, and 640 million cfu / g of Streptomyces roseo.

[0042] 2. Preparation of microbial fertilizer

[0043] First, 10 g of sodium alginate was heated to a molten state, and then added to 15 g of polyethylene glycol, mixed, and dried to obtain a mixture for later use;

[0044] Secondly, add water to the mixture obtained above and stir until it is in a gel state, then add 8g of the above-mentioned microbial agent, mix evenly, dry, add 10g of sugarcane bagasse powder and 10g of attapulgite, mix evenly, and obtain microbial fertilizer.

[0045] Example 3 Microbial Fertilizer

[0046] 1. Preparation of microbial agents

[0047] Preparation of fermentation medium: First, 1 kg of discarded bones was crushed and an equal weight of water was added to form a suspension, 0.6 mg of Saccharomyces boulardii (commercially available with an activity of 20 billion cfu / g of bacterial powder) and 1.2 mg of acetic acid bacteria (commercially available with an activity of 3 billion cfu / g of bacterial powder) were added, and fermented at 30-35°C for 1-1.5 days. After fermentation, the mixture was sterilized at high temperature and the fermentation product was dried to obtain fermented bone residue powder; then, a fermentation medium was prepared according to the ratio of 16 g / L of fermented bone residue powder, 9 g / L of mulberry linterinary polypeptide, 23 g / L of broken yeast powder, 4 g / L of carotene, 18 g / L of wheat bran powder, 6 g / L of chicory polysaccharide, 5 g / L of calcium carbonate, and 0.7 g / L of potassium dihydrogen phosphate, and the pH was adjusted to 7.0;

[0048] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.47; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.58; Bacillus megaterium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The seed solution was 0.47; Penicillium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The Streptomyces roseus strain was activated according to the strain instructions and placed in the seed culture medium to culture until the OD 600 is 0.6, and the seed solution is obtained;

[0049] Production: The above seed liquid is inoculated into the above-obtained fermentation medium according to the inoculum amount of 4 mL / L of Bacillus gelatinosa, 6 mL / L of Bacillus circulans, 2 mL / L of Bacillus megaterium, 0.6 mL / L of Penicillium, and 0.8 mL / L of Streptomyces roseus, respectively. Ferment for 1 day. The fermentation product is freeze-dried and then crushed to obtain the microbial agent.

[0050] The obtained microbial agent was tested and found to contain 3.84 billion cfu / g of Bacillus gelatinosa, 4.07 billion cfu / g of Bacillus circulans, 1.46 billion cfu / g of Bacillus megaterium, 490 million cfu / g of Penicillium, and 830 million cfu / g of Streptomyces roseo.

[0051] 2. Preparation of microbial fertilizer

[0052] First, 10-15g of sodium alginate is heated to a molten state, then added to 15-20g of polyethylene glycol, mixed, and dried to obtain a mixture for later use;

[0053] Secondly, add water to the mixture obtained above and stir until it is in a gel state, then add 8-10g of the above-mentioned microbial agent, mix evenly, dry, add 10-15g of sugarcane bagasse powder and 5-10g of attapulgite, and mix evenly to obtain microbial fertilizer.

[0054] Comparative Example 1 Microbial Fertilizer

[0055] 1. Preparation of microbial agents

[0056] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.55; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.51; after Bacillus megaterium was activated according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 is 0.43, and the seed solution is obtained;

[0057] Production: The above seed liquids are inoculated into a fermentation medium containing 16 g / L of bone residue powder, 9 g / L of mulberry linterinary polypeptide, 23 g / L of broken yeast powder, 4 g / L of carotene, 18 g / L of wheat bran powder, 6 g / L of chicory polysaccharide, 5 g / L of calcium carbonate, and 0.7 g / L of dipotassium hydrogen phosphate at a pH of 7.0 according to the inoculation amount of 4 mL / L of gelatinous Bacillus, 6 mL / L of circulans, and 2 mL / L of Bacillus megaterium, respectively. The fermentation is carried out for 1-1.5 days. The fermentation product is freeze-dried and then crushed to obtain a microbial agent.

[0058] The obtained microbial agent was tested and found to contain 1.61 billion cfu / g of Bacillus gelatinosa, 2.16 billion cfu / g of Bacillus circulans, and 1.53 billion cfu / g of Bacillus megaterium.

[0059] 2. Preparation of microbial fertilizer

[0060] First, 13 g of sodium alginate was heated to a molten state, and then added to 18 g of polyethylene glycol, mixed, and dried to obtain a mixture for later use;

[0061] Secondly, add water to the mixture obtained above and stir until it is in a gel state, then add 9g of the above-mentioned microbial agent, mix evenly, dry, add 13g of sugarcane bagasse powder and 7g of attapulgite, mix evenly, and obtain microbial fertilizer.

[0062] Comparative Example 2 Microbial Fertilizer

[0063] 1. Preparation of microbial agents

[0064] Preparation of fermentation medium: First, 1 kg of discarded bones was crushed and an equal weight of water was added to form a suspension, 0.6 mg of boulardii yeast (commercially available activity is 20 billion cfu / g of bacterial powder) was added, and fermented at 30-35°C for 1-1.5 days. After fermentation, the mixture was sterilized at high temperature and the fermentation product was dried to obtain fermented bone residue powder; then, a fermentation medium was prepared according to the ratio of 16 g / L of fermented bone residue powder, 9 g / L of mulberry linterinary polypeptide, 23 g / L of broken yeast powder, 4 g / L of carotene, 18 g / L of wheat bran powder, 6 g / L of chicory polysaccharide, 5 g / L of calcium carbonate, and 0.7 g / L of potassium dihydrogen phosphate, and the pH was adjusted to 6.5-7.5;

[0065] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.0.57; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.43; the Streptomyces roseus strain was activated according to the strain instructions and placed in the seed culture medium to culture until the OD 600 is 0.51, and the seed solution is obtained;

[0066] Production: The above seed liquid is inoculated into the above-obtained fermentation medium according to the inoculum amount of 4 mL / L of Bacillus gelatinosa, 6 mL / L of Bacillus circulans, and 0.8 mL / L of Streptomyces roseus, respectively. The fermentation is carried out for 1-1.5 days. The fermentation product is freeze-dried and then crushed to obtain the microbial agent.

[0067] The obtained microbial agent was tested and found to contain 4.83 billion cfu / g of Bacillus gelatinosa, 2.17 billion cfu / g of Bacillus circulans, and 180 million cfu / g of Streptomyces roseo.

[0068] 2. Preparation of microbial fertilizer

[0069] Dissolve 13g of sodium alginate in water, add 18g of polyethylene glycol and 9g of the above-mentioned microbial agent, mix evenly, dry, add 13g of bagasse powder and 7g of attapulgite, mix evenly, and obtain microbial fertilizer.

[0070] Comparative Example 3 Microbial Fertilizer

[0071] 1. Preparation of microbial agents

[0072] Preparation of fermentation medium: First, 1 kg of discarded bones is crushed and an equal weight of water is added to form a suspension. Then, 0.6 mg of Saccharomyces boulardii (commercially available with an activity of 20 billion cfu / g) and 1.2 mg of acetic acid bacteria (commercially available with an activity of 3 billion cfu / g) are added. The mixture is fermented at 30-35°C for 1-1.5 days. After fermentation, the mixture is sterilized at high temperature and the fermentation product is dried to obtain fermented bone residue powder. Then, a fermentation medium is prepared according to the ratio of 16 g / L fermented bone residue powder, 18 g / L wheat bran powder, 6 g / L chicory polysaccharide, 5 g / L calcium carbonate, and 0.7 g / L potassium hydrogen phosphate, and the pH is adjusted to 6.5-7.5.

[0073] Seed solution preparation: Take the jelly-like Bacillus and activate it according to the strain instructions, then place it in the seed culture medium and culture it until OD 600 The seed solution was 0.52; after activating Bacillus circulans according to the strain instructions, it was placed in the seed culture medium and cultured until the OD 600 The seed solution was 0.63; Bacillus megaterium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The seed solution was 0.39; Penicillium was activated according to the strain instructions and placed in the seed culture medium to culture until OD 600 The Streptomyces roseus strain was activated according to the strain instructions and placed in the seed culture medium to culture until the OD 600 is 0.55, and the seed solution is obtained;

[0074] Production: The above seed liquid is inoculated into the above-obtained fermentation medium according to the inoculum amount of 4 mL / L of Bacillus gelatinosa, 6 mL / L of Bacillus circulans, 2 mL / L of Bacillus megaterium, 0.6 mL / L of Penicillium, and 0.8 mL / L of Streptomyces roseus, respectively. The fermentation is carried out for 1-1.5 days. The fermentation product is freeze-dried and then crushed to obtain the microbial agent.

[0075] The obtained microbial agent was tested and found to contain 2.68 billion cfu / g of Bacillus gelatinosa, 3.32 billion cfu / g of Bacillus circulans, 380 million cfu / g of Bacillus megaterium, 590 million cfu / g of Penicillium, and 270 million cfu / g of Streptomyces roseo.

[0076] 2. Preparation of microbial fertilizer

[0077] First, 13 g of sodium alginate was heated to a molten state, and then added to 18 g of polyethylene glycol, mixed, and dried to obtain a mixture for later use;

[0078] Secondly, add water to the mixture obtained above and stir until it is in a gel state, then add 9g of the above-mentioned microbial agent, mix evenly, dry, add 13g of sugarcane bagasse powder and 7g of attapulgite, mix evenly, and obtain microbial fertilizer.

[0079] Performance Testing

[0080] 1. Improvement of farmland soil compaction by the microbial fertilizer of the present invention

[0081] 1.4 mu of land with soil compaction in Linshu County was selected and randomly divided into 1-7 groups, each with 0.2 mu. Among them, 6 groups used the microbial fertilizer obtained in Examples 1-3 and Comparative Examples 1-3 for six consecutive months, and recorded the soil conditions; potatoes were then planted in the soil, and the potato growth and yield were recorded; the remaining group did not apply microbial fertilizer and served as the control group.

[0082] Table 1 Improvement of soil compaction

[0083]

[0084] As can be seen from the results in Table 1, the use of the microbial fertilizer of the present invention can effectively improve soil compaction, increase the aggregate structure in the soil, improve the looseness and air permeability, and increase the fertility of the soil. When used for potato planting, it can effectively increase the potato emergence rate and the overall potato yield.

[0085] 2. Effect of the microbial fertilizer of the present invention on soil properties

[0086] One month before corn planting, the microbial fertilizers obtained in Examples 1-3 and Comparative Examples 1-3 were applied to the soil, and the shape of the soil was measured after the corn was harvested.

[0087] Table 2 Soil conditions of corn planting areas

[0088]

[0089] Table 3 Microbial activity in soil of corn planting area

[0090] Group microbial fertilizer <![CDATA[Bacteria (×10 6 cfu / g)]]> <![CDATA[Fungus (×10 5 cfu / g)]]> <![CDATA[Actinomycetes (×10 6 cfu / g) <!-- 7 -->]]> 1 group Example 1 12.58 7.86 6.68 2 groups Example 2 13.07 8.49 8.34 3 groups Example 3 11.36 6.35 7.94 4 groups Comparative Example 1 10.21 4.97 4.62 5 groups Comparative Example 2 9.06 4.38 5.37 6 groups Comparative Example 3 8.67 3.64 4.93 7 groups Not used 7.28 2.06 4.01

[0091] It can be seen from the data in Table 2 that the soil bulk density of the microbial fertilizer obtained by using the present invention is reduced, the total porosity and aeration porosity are increased, and the soil organic matter content and total nitrogen content are also increased. It can be seen that the microbial fertilizer of the present invention can promote the improvement of the physical structure of the soil, and the increase in organic matter content and total nitrogen content is more conducive to the formation of soil aggregates; in addition, the use of microbial fertilizer can enrich the types of microorganisms in the soil, while increasing the activity of the original microorganisms in the soil, promoting their growth and reproduction, and being more conducive to the formation of water-stable aggregate structure. At the same time, substances such as organic acids produced during microbial metabolism can dissolve fixed salt ions in the soil, thereby preventing the soil from becoming compacted.

Claims

1. A microbial compound fertilizer, characterized in that: The unit is g, containing 8-10g of microbial agents and 40-60g of non-microbial raw materials; The non-microbial raw material contains 10-15g of sodium alginate, 15-20g of polyethylene glycol, 10-15g of bagasse powder, and 5-10g of attapulgite; The microbial agent contains 3.02-4.47 billion cfu / g of Bacillus gelatinosa, 4.07-4.86 billion cfu / g of Bacillus circulans, 1.02-1.46 billion cfu / g of Bacillus megaterium, 490-970 million cfu / g of Penicillium, and 310-830 million cfu / g of Streptomyces roseus. The preparation method of the microbial agent comprises the following steps: Step A: After activating the strains of Bacillus jelly, Bacillus circulans, Bacillus megaterium, Penicillium and Streptomyces roseus, they were placed in seed culture medium and cultured until OD 600 is 0.4-0.6, and the seed solution is obtained; Step B, inoculating the seed liquid into a fermentation medium containing 15-18 g / L of fermented bone residue powder, 8-10 g / L of mulberry linterinary polypeptide, 20-25 g / L of broken yeast powder, 3-5 g / L of carotene, 15-20 g / L of wheat bran powder, 5-8 g / L of chicory polysaccharide, 3-6 g / L of calcium carbonate, and 0.5-0.8 g / L of dipotassium hydrogen phosphate, with a pH of 6.5-7.5, fermenting for 1-1.5 days, and freeze-drying and crushing the fermentation product to obtain a microbial agent; in, In step A of the microbial agent preparation method, the inoculum amount of the Bacillus gelatinosa seed solution is 3-5 mL / L, the inoculum amount of the Bacillus circulans seed solution is 4-8 mL / L, the inoculum amount of the Bacillus megaterium seed solution is 1.5-3 mL / L, the inoculum amount of the Penicillium seed solution is 0.5-0.8 mL / L, and the inoculum amount of the Streptomyces roseoensis seed solution is 0.5-1 mL / L; The preparation method of the microbial agent step B is as follows: Discarded bones are crushed and added with an equal weight of water to form a suspension. 0.5-0.8% of the weight of the bones by Saccharomyces boulardii and 0.1-0.12% of acetic acid bacteria are added. The suspension is fermented at 30-35°C for 1-1.5 days. After fermentation, the suspension is sterilized at high temperature and the fermentation product is dried to obtain fermented bone residue powder. The activity of the Saccharomyces boulardii is 20 billion cfu / g, and the activity of the acetic acid bacteria is 3 billion cfu / g.

2. A method for preparing the microbial composite fertilizer according to claim 1, characterized in that: The preparation method comprises the following steps: Step 1: adding molten sodium alginate to polyethylene glycol, mixing and drying to obtain a mixture for later use; Step 2: Add water to the mixture obtained in step 1 and stir until it is in a gel state, then add the microbial raw materials, mix evenly, dry, add bagasse powder and attapulgite, and mix evenly to obtain the microbial compound fertilizer.

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