A preparation method of o-phthalaldehyde disinfectant with concentration indication function

Abstract

The present invention proposes a preparation method of an o-phthalaldehyde disinfectant with a concentration indication function, comprising the following steps: S1, preparing a buffer solution: mixing a phosphate solution with a strong alkaline solution, diluting and shaking; S2, preparing a color developer: dissolving ninhydrin with n-butanol, diluting with water, adding thymol blue, shaking and standing; S3, adding the prepared buffer solution and color developer to the disinfectant stock solution, shaking and standing; S4, adding sodium sulfite to the disinfectant prepared in step S3, adjusting the effective content concentration, standing, and observing the color change. The o-phthalaldehyde disinfectant prepared by the present invention has a concentration indication function, and can change color according to the concentration change of its effective ingredient, so as to assist the user to judge whether the disinfectant can continue to be used more quickly, intuitively and accurately; at the same time, it can also prevent the disinfectant contamination problem caused by the frequent use of detection tools, as well as the cost problem of detection tool consumables.

Description

Technical Field

[0001] The invention relates to the technical field of medical disinfection, in particular to a method for preparing an o-phthalaldehyde disinfectant with a concentration indication function. Background Art

[0002] O-phthalaldehyde was first used as a dye, but later its excellent bactericidal properties were discovered, and it was approved by the FDA in 1999 as a high-level disinfectant. It is mainly used for immersion disinfection of heat-sensitive diagnostic and treatment instruments, appliances and items.

[0003] During the use of o-phthalaldehyde, its minimum effective concentration is 0.3%, and its concentration is usually measured using the manufacturer's test paper. Although this method of operation is simple and quick, it also has many problems: first, it is difficult to grasp the accuracy in time, and too long or too short contact and reaction in the air may cause medical staff to make mistakes in concentration judgment; second, the color card is not very accurate, and the general test paper only has four color cards for comparison; third, the existence of the test paper will increase the possibility of medical equipment being contaminated in disguise; and the test paper is a disposable item, and the use of consumables is also a considerable expense for the hospital.

[0004] Therefore, in view of the shortcomings of the existing technology, there is an urgent need for a phthalaldehyde disinfectant that can efficiently distinguish the concentration and can more intuitively, accurately and quickly determine whether the disinfectant is ineffective without using a test paper. Summary of the invention

[0005] In order to solve the problems and defects of the prior art, the purpose of the present invention is to provide a method for preparing an o-phthalaldehyde disinfectant with a concentration indication function, so as to solve the problem that the effective content concentration of the o-phthalaldehyde disinfectant often needs to be detected before use and additional consumables are required.

[0006] In order to achieve the above object, the present invention proposes a method for preparing an o-phthalaldehyde disinfectant with a concentration indication function, comprising the following steps:

[0007] S1. Prepare buffer solution: Mix phosphate solution and strong alkaline solution, dilute and shake well;

[0008] S2. Prepare the color developer: dissolve ninhydrin in n-butanol, dilute with water, add thymol blue, shake well and let stand;

[0009] S3. Add the prepared buffer solution and color developer to the disinfectant stock solution, shake well and let stand. At this time, the disinfectant is colorless and transparent or light yellow and transparent;

[0010] S4. Add sodium sulfite to the disinfectant prepared in step S3 to adjust the concentration of the active ingredient in the disinfectant. Let it stand and observe the color change of the disinfectant. The color of the disinfectant will gradually darken as the concentration of the active ingredient decreases.

[0011] The o-phthalaldehyde disinfectant prepared by the present invention has a concentration indication function, and can change color according to the concentration change of its effective ingredients, thereby helping users to more quickly, intuitively and accurately judge whether the disinfectant can continue to be used; at the same time, it can also prevent the disinfectant contamination problem caused by the frequent use of detection tools, as well as the cost problem of detection tool consumables.

[0012] Preferably, in step S1, the phosphate can be any one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, and disodium hydrogen phosphate, or the above phosphates can be randomly mixed in pairs; the strong base can be any one of sodium hydroxide and potassium hydroxide, or the above strong bases can be mixed and used.

[0013] Preferably, in step S1, the phosphate is potassium dihydrogen phosphate and the strong base is potassium hydroxide.

[0014] Preferably, the concentration of the phosphate solution in step S1 is 0.1 mol / L to 0.5 mol / L; the concentration of the strong alkali solution in step S1 is 0.1 mol / L to 0.5 mol / L.

[0015] Preferably, the amount of ninhydrin used in step S2 is 0.1wt% to 0.6wt% of the effective ingredients of the disinfectant.

[0016] Preferably, the amount of thymol blue used in step S2 is 0.01wt% to 0.1wt% of the effective ingredient of the disinfectant.

[0017] Preferably, in step S3, the mass ratio of the disinfectant stock solution to the added buffer solution is 5:1 to 10:1; and the mass ratio of the disinfectant stock solution to the added color developer is 5:1 to 10:1.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The o-phthalaldehyde disinfectant prepared by the present invention has a concentration indication function, and can change color according to the concentration change of its effective ingredient, thereby assisting the user to more quickly, intuitively and accurately judge whether the disinfectant can continue to be used.

[0020] 2. The o-phthalaldehyde disinfectant prepared by the present invention solves the problem that the o-phthalaldehyde disinfectant needs to be frequently tested with test paper before use. The disinfectant will change color from green to blue as its concentration decreases, thereby helping the user to intuitively judge whether the disinfectant can still be used.

[0021] 3. The reagents used in the preparation process of the o-phthalaldehyde disinfectant prepared by the present invention are all non-toxic and the added amount is low, which will not affect the sterilization effect of the disinfectant.

[0022] 4. Since the o-phthalaldehyde disinfectant prepared by the present invention does not require the detection assistance of test paper, it can prevent the problem of reagent contamination and reduce the cost of consumables used in detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 The present invention is a schematic flow chart of a method for preparing an o-phthalaldehyde disinfectant with a concentration indication function. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. The components of the embodiments of the present invention generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] The present invention provides a method for preparing an o-phthalaldehyde disinfectant with a concentration indication function, comprising the following steps:

[0027] S1. Prepare phosphate buffer: take 250 mL of 0.2 mol / L potassium dihydrogen phosphate solution, add 118 mL of 0.2 mol / L sodium hydroxide solution, dilute with water to 1000 mL, and shake well;

[0028] S2. Prepare a color developer: dilute ninhydrin to 100 ml with water, and then add thymol blue. Preferably, the amount of ninhydrin is 0.1 wt% to 0.6 wt% of the active ingredient of the disinfectant, and the amount of thymol blue is 0.01 wt% to 0.1 wt% of the active ingredient of the disinfectant;

[0029] S3. Add the prepared buffer solution and color developer to the disinfectant stock solution, wherein the mass ratio of the disinfectant stock solution to the added buffer solution is 5:1 to 10:1; and the mass ratio of the disinfectant stock solution to the added color developer is 5:1 to 10:1; shake well and let stand; at this time, the disinfectant is colorless and transparent or light yellow and transparent;

[0030] S4. Add sodium sulfite to the disinfectant prepared in step S3 to adjust the concentration of the active ingredient in the disinfectant. Let it stand and observe the color change of the disinfectant. The color of the disinfectant will gradually darken as the concentration of the active ingredient decreases.

[0031] The present invention uses the oxidizing property and weak acidity of o-phthalaldehyde, and the principle of color change of ninhydrin and amino acid reaction. When o-phthalaldehyde is consumed, medical devices can no longer be disinfected, and the residual protein and bacteria react with ninhydrin to make the disinfectant present different colors, thereby intuitively judging the concentration of the disinfectant without the assistance of test paper. The materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be purchased from commercial channels.

[0032] Example 1

[0033] 1. Prepare phosphate buffer: take 250 mL of 0.2 mol / L potassium dihydrogen phosphate solution, add 118 mL of 0.2 mol / L sodium hydroxide solution, dilute with water to 1000 mL, and shake well;

[0034] 2. Prepare the color developer: take 0.1g of ninhydrin and dilute to 100ml with water, then add 10 drops of thymol blue;

[0035] 3. Take 9 ml of 0.6% o-phthalaldehyde stock solutions A1, B1, C1, and D1 respectively, and add 0.5 ml of color developer and 0.5 ml of phosphate buffer to A1, B1, C1, and D1 respectively, shake well, and let stand; observe the color of the four solutions. At this time, the solutions of o-phthalaldehyde stock solutions A1, B1, C1, and D1 are all colorless and transparent;

[0036] 4. Add 7.2 mg of sodium sulfite to B1, add 14.4 mg of sodium sulfite to C1, and add 28.7 mg of sodium sulfite to D1, shake well, and let stand; then, B1 changes from colorless to yellow, C1 changes from colorless to yellowish brown, and D1 changes from colorless to brown.

[0037] The experimental results of this embodiment show that when the content of the active ingredient o-phthalaldehyde in the o-phthalaldehyde disinfectant with a concentration indication function of the present application decreases, the color of the disinfectant will gradually darken. When the color changes from colorless to dark brown, the disinfectant of the present application can no longer be used for sterilization and disinfection. It can be seen from this that the o-phthalaldehyde disinfectant with a concentration indication function proposed in the present application can clearly and intuitively judge the concentration state of the disinfectant through color changes. The lower the concentration of the active ingredient o-phthalaldehyde in the disinfectant, the darker the color of the disinfectant; when the color changes to dark brown, it can be clearly seen that the disinfectant has failed.

[0038] Example 2

[0039] 1. Prepare phosphate buffer: take 250 mL of 0.2 mol / L potassium dihydrogen phosphate solution, add 118 mL of 0.2 mol / L sodium hydroxide solution, dilute with water to 1000 mL, and shake well;

[0040] 2. Prepare the color developer: take 0.2g of ninhydrin and dilute to 100ml with water, then add 10 drops of thymol blue;

[0041] 3. Take 9 ml of 0.6% o-phthalaldehyde stock solutions A2, B2, C2, and D2 respectively, and add 0.5 ml of color developer and 0.5 ml of phosphate buffer to A2, B2, C2, and D2 respectively, shake well, and let stand; observe the color of the four solutions. At this time, the solutions of o-phthalaldehyde stock solutions A2, B2, C2, and D2 are all colorless and transparent;

[0042] 4. Add 7.2 mg of sodium sulfite to B2, add 14.4 mg of sodium sulfite to C2, and add 28.7 mg of sodium sulfite to D2. Shake well and let stand. Subsequently, B2 changes from colorless to yellow, C2 changes from colorless to yellowish brown, and D2 changes from colorless to brown.

[0043] The experimental results of this embodiment show that: the concentration change of ninhydrin will not affect the color change state of the o-phthalaldehyde disinfectant with concentration indication function of the present application; when the content of o-phthalaldehyde, an effective ingredient, in the o-phthalaldehyde disinfectant with concentration indication function of the present application is reduced, the color of the disinfectant will gradually become darker, and when the color changes from colorless to dark brown, the disinfectant of the present application can no longer be used for sterilization and disinfection. It can be seen from this that the o-phthalaldehyde disinfectant with concentration indication function proposed in the present application can clearly and intuitively judge the concentration state of the disinfectant through color changes. The lower the concentration of o-phthalaldehyde, an effective ingredient of the disinfectant, the darker the color of the disinfectant; when the color changes to dark brown, it can be clearly seen that the disinfectant has failed.

[0044] Example 3

[0045] 1. Prepare phosphate buffer: take 250 mL of 0.2 mol / L potassium dihydrogen phosphate solution, add 118 mL of 0.2 mol / L sodium hydroxide solution, dilute with water to 1000 mL, and shake well;

[0046] 2. Prepare the color developer: take 0.2g of ninhydrin and dilute to 100ml with water, then add 15 drops of thymol blue;

[0047] 3. Take 9 ml of 0.6% o-phthalaldehyde stock solution A3, B3, C3, and D3 respectively, and add 0.5 ml of color developer and 0.5 ml of phosphate buffer to A3, B3, C3, and D3 respectively, shake well, and let stand; observe the color of the four solutions. At this time, the solutions of o-phthalaldehyde stock solution A3, B3, C3, and D3 are all colorless and transparent;

[0048] 4. Add 7.2 mg of sodium sulfite to B3, add 14.4 mg of sodium sulfite to C3, and add 28.7 mg of sodium sulfite to D3. Shake well and let stand. Subsequently, B3 changes from colorless to yellow, C3 changes from colorless to gray-black, and D3 changes from colorless to black.

[0049] The experimental results of this embodiment show that the color change state of the o-phthalaldehyde disinfectant with a concentration indication function of the present application will change with the concentration change of thymol blue; when the content of o-phthalaldehyde, an effective ingredient, in the o-phthalaldehyde disinfectant with a concentration indication function of the present application is reduced, the color of the disinfectant will gradually become darker, and when the color changes from colorless to black, the disinfectant of the present application can no longer be used for sterilization and disinfection. It can be seen from this that the o-phthalaldehyde disinfectant with a concentration indication function proposed in the present application can clearly and intuitively judge the concentration state of the disinfectant through color changes. The lower the concentration of o-phthalaldehyde, an effective ingredient of the disinfectant, the darker the color of the disinfectant; when the color changes to black, it can be clearly seen that the disinfectant has failed.

[0050] The experimental results obtained by adjusting the parameter ratios of Examples 1-3 show that within the concentration range of the disinfectant in Examples 1-3, the o-phthalaldehyde disinfectant configured by the present application can achieve the function of concentration indication through color changes.

[0051] Of course, the above description is only a specific embodiment of the present invention and is not intended to limit the scope of implementation of the present invention. All equivalent changes or modifications made according to the structure, characteristics and principles described in the patent application scope of the present invention should be included in the patent application scope of the present invention.

[0052] Finally, it should be noted that the above-described embodiments are only specific implementations of the present invention, which are used to illustrate the technical solutions of the present invention, rather than to limit them. The protection scope of the present invention is not limited thereto. Although the present invention is described in detail with reference to the above-described embodiments, ordinary technicians in the field should understand that any technician familiar with the technical field can still modify the technical solutions recorded in the above-described embodiments within the technical scope disclosed by the present invention, or can easily think of changes, or make equivalent replacements for some of the technical features therein; and these modifications, changes or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention, and should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A method for preparing an o-phthalaldehyde disinfectant with a concentration indication function, characterized in that: The steps include: S1. Prepare buffer solution: Mix phosphate solution and strong alkaline solution, dilute and shake well; S2. Prepare the color developer: dissolve ninhydrin in n-butanol, dilute with water, add thymol blue, shake well and let stand; S3. Add the prepared buffer solution and color developer to the disinfectant stock solution, shake well and let stand. At this time, the disinfectant is colorless and transparent or light yellow and transparent; S4, adding sodium sulfite to the disinfectant prepared in step S3 to adjust the concentration of the active ingredient in the disinfectant, standing and observing the color change of the disinfectant, the color of the disinfectant will gradually darken as the concentration of the active ingredient decreases; In step S1, the phosphate can be selected from any one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, and disodium hydrogen phosphate, or the above phosphates can be randomly mixed in pairs; the strong base can be selected from any one of sodium hydroxide and potassium hydroxide, or the above strong bases can be mixed and used; The concentration of the phosphate solution in step S1 is 0.1 mol / L to 0.5 mol / L; the concentration of the strong alkali solution in step S1 is 0.1 mol / L to 0.5 mol / L; The amount of ninhydrin used in step S2 is 0.1wt% to 0.6wt% of the effective ingredient of the disinfectant; The amount of thymol blue used in step S2 is 0.01wt% to 0.1wt% of the effective ingredient of the disinfectant; In step S3, the mass ratio of the disinfectant stock solution to the added buffer solution is 5:1 to 10:1; and the mass ratio of the disinfectant stock solution to the added color developer is 5:1 to 10:

1.

2. The method for preparing a phthalaldehyde disinfectant with a concentration indicating function according to claim 1, characterized in that: In step S1, potassium dihydrogen phosphate is used as the phosphate, and potassium hydroxide is used as the strong base.

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