Sterilization glass slide convenient for gram staining and identification

Abstract

The utility model provides a sterilization glass slide convenient for gram staining and identification, which comprises a glass slide main body, a sample information marking area, a standard bacterium area and a sample bacterium area are sequentially arranged on the front surface of the glass slide main body, and the sterilization glass slide is characterized in that the sample information marking area, the standard bacterium area and the sample bacterium area are sequentially arranged along the length direction of the glass slide main body; the front surface of the glass slide main body is partitioned through partition straight lines coated with the front surface of the glass slide main body, the partition straight lines are arranged in the width direction of the glass slide main body, and the multiple partition straight lines are sequentially distributed at intervals in the length direction of the glass slide main body; separation round lines are respectively arranged in the standard bacterium area and the sample bacterium area; and the thicknesses d of the separation straight lines and the separation round lines are 0.5-2 mm, and the widths D of the separation straight lines and the separation round lines are 0.5-2 mm. According to the utility model, the cross contamination problem in the staining process can be effectively improved, false positive and false negative phenomena are avoided, the accuracy of a staining result is improved, and the operation convenience, the result accuracy and the test efficiency of gram staining and identification of sample bacteria are improved.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection technology, and in particular to a sterile glass slide that is easy to Gram stain and identify. Background Technology

[0002] Gram staining is a classic method for bacterial classification and identification, widely used in microbiology research, clinical diagnosis, food safety, and agriculture. Traditional Gram staining methods mainly include steps such as smear preparation, fixation, primary staining, mordanting, destaining, and counterstaining. However, existing slide designs are relatively simple, usually using ordinary glass slides, and are not specifically optimized for Gram staining, resulting in the following shortcomings: Traditional slides lack detailed partitioning, typically only indicating sample information marking areas and sample bacterial areas, and using only lines. These lines only show the partition locations and cannot effectively separate the sample information marking areas from the sample bacterial areas. This easily leads to bacterial displacement during staining, uneven stain coverage, and cross-contamination from stain overflow into other areas. This not only contaminates the marking information but also risks insufficient staining in the sample bacterial areas and allows bacteria to contaminate other strains with spilled staining, thus affecting the accuracy of the results and causing false positives or false negatives. Therefore, improvement is necessary. Summary of the Invention

[0003] The purpose of this invention is to provide a sterile glass slide that facilitates Gram staining and identification. This slide effectively improves the problem of cross-contamination during the staining process, avoids false positives and false negatives, and improves the accuracy of staining results. Ultimately, this invention enhances the ease of operation, accuracy of results, and efficiency of Gram staining and identification of bacterial samples.

[0004] The objective of this utility model can be achieved through the following technical solutions:

[0005] A sterile glass slide for easy Gram staining and identification includes a long, strip-shaped slide body. The front side of the slide body is sequentially provided with a sample information marking area, a standard bacterial area, and a sample bacterial area. The slide body is characterized in that: the sample information marking area, the standard bacterial area, and the sample bacterial area are arranged sequentially along the length of the slide body and are divided into sections by dividing lines applied to the front side of the slide body. These dividing lines are arranged along the width of the slide body, and multiple dividing lines are sequentially spaced along the length of the slide body. Separating circular lines are also provided within the standard bacterial area and the sample bacterial area. The thickness d of the dividing lines and the dividing circular lines is 0.5 to 2 mm, and the width D is 0.5 to 2 mm.

[0006] The optimized scheme includes two standard bacterial zones: a positive standard bacterial zone and a negative standard bacterial zone.

[0007] Furthermore, Gram-positive bacteria are arranged within the dividing circle of the positive standard bacterial area, and Gram-negative bacteria are arranged within the dividing circle of the negative standard bacterial area.

[0008] In the optimized scheme, the dividing circle has one overflow notch or multiple evenly distributed overflow notches.

[0009] Furthermore, the dividing circle in the standard bacterial area has two symmetrically arranged overflow notches to form a standard bacterial dividing line, and the dividing circle in the sample bacterial area has four to eight evenly distributed overflow notches to form a sample bacterial dividing line.

[0010] The optimized scheme includes two or more bacterial zones in the sample.

[0011] In the optimized design, both the dividing straight lines and dividing circles are formed using epoxy paint.

[0012] This utility model has the following substantial features and advancements:

[0013] 1. This utility model designs a sterile glass slide specifically for Gram staining and bacterial identification. It enables the marking of sample information and the placement of standard bacteria and sample bacteria on the same glass slide, thereby achieving intuitive and effective comparison and analysis, reducing the phenomenon of "false positives" and "false negatives", improving the accuracy of staining results, and enhancing the ease of operation, accuracy of results, and efficiency of Gram staining and identification of sample bacteria.

[0014] 2. This utility model effectively avoids cross-contamination by using the combination of dividing circles and dividing lines to form a double limiting effect, reducing the requirements for operators, improving operating efficiency, and thus quickly identifying bacterial types and shortening detection time, making it suitable for users with different technical levels. Attached Figure Description

[0015] Figure 1 This is a top-view structural diagram of the sterilization slide of this utility model.

[0016] Figure 2 for Figure 1 Enlarged view of part A in the middle.

[0017] Figure 3 This is a schematic diagram of the sterilization slide of this utility model in the main view.

[0018] Figure 4 for Figure 3 Enlarged view of section B in the middle. Detailed Implementation

[0019] The present invention will be further described below with reference to the accompanying drawings.

[0020] Example

[0021] refer to Figures 1 to 4 A sterile glass slide for easy Gram staining and identification includes a long, strip-shaped slide body 1. The front side of the slide body 1 is sequentially provided with a sample information marking area 11, a standard bacterial area, and a sample bacterial area 13. The standard bacterial area includes two areas: a positive standard bacterial area 121 and a negative standard bacterial area 122.

[0022] The sample information marking area 11, the standard bacteria area, and the sample bacteria area 13 are arranged sequentially along the length of the slide body 1, and are divided into sections by dividing lines 2 applied to the front of the slide body 1. The dividing lines 2 are arranged along the width of the slide body 1, and multiple dividing lines 2 are distributed sequentially at intervals along the length of the slide body 1.

[0023] Separating circular lines are also provided in the standard bacterial area and the sample bacterial area 13, respectively; the thickness d of the separating straight line 2 and the separating circular lines is 0.5 to 2 mm, and the width D is 0.5 to 2 mm. In this embodiment, the thickness d is specifically 1 mm and the width D is specifically 1 mm.

[0024] In the above structure, the sample information marking area 11 is used to mark relevant sample information, such as sample number and date. The standard bacteria area is used to place known standard strains. Specifically, the dividing lines of the positive standard bacteria area 121 are used to place Gram-positive bacteria, and the dividing lines of the negative standard bacteria area 122 are used to place Gram-negative bacteria for comparison and verification of staining results. The dividing lines of the sample bacteria area 13 are used to place the sample strain to be tested.

[0025] The usage method of the above structure is as follows: 1. Sterilization: Before use, sterilize the slide to ensure a sterile state. 2. Sample preparation: Mark the relevant information of the sample in the sample information marking area. Apply standard bacterial strains and sample bacterial strains to be tested to the dividing lines of the standard bacterial area and the sample bacterial area, respectively. This ensures the strains are coated within the dividing lines, which clearly identify and limit the coating position and extent of the strains. 3. Staining process: Stain the standard bacterial strains and sample bacterial strains on the slide simultaneously according to the Gram staining method. During the staining process, the staining solution is confined within the dividing lines, thus reducing the risk of staining spillage. Even if a small amount of staining solution spills, it will be further confined by the dividing line 2, preventing contamination of nearby areas. 4. Result observation and analysis: Observe the staining results, compare the staining results of the standard bacterial area and the sample bacterial area, and perform bacterial identification.

[0026] In the above structure, sample information labeling, standard bacteria, and sample bacteria are stained simultaneously on a single glass slide. The Gram staining test of standard bacteria and sample bacteria is double-limited by the dividing line 2 and the dividing circle, which effectively avoids cross-contamination. The standard bacteria include both Gram-positive and Gram-negative bacteria. By staining simultaneously with the standard bacteria, the difference in results caused by the details of Gram staining time is eliminated, which facilitates comparative observation and improves the convenience, accuracy, and efficiency of Gram staining and identification of sample bacteria.

[0027] Furthermore, the Gram-positive bacteria arranged within the dividing lines of the positive standard bacterial area 121 and the Gram-negative bacteria arranged within the dividing lines of the negative standard bacterial area 122 in this embodiment can be subjected to Gram staining tests such as partitioning and staining along with the sample bacteria, as described above. Alternatively, during the sterilization slide manufacturing process, smearing, drying, and fixing operations can be performed sequentially within the dividing lines of the positive standard bacterial area 121 and the negative standard bacterial area 122 in advance to pre-set the positive and negative bacteria, and the positive standard bacterial area 121 and the negative standard bacterial area 122 can be protected with a sterile protective film. Then, during use, they can be stained along with the sample bacteria.

[0028] Furthermore, the dividing circle has one or more evenly distributed overflow notches 30. The function of the overflow notches 30 is to achieve rapid balance inside and outside the dividing circle, so that the dye solution dripped into the dividing circle is quickly distributed within the dividing circle under the balancing effect of the overflow notches 30, reducing uneven coverage of the dye solution, thereby enabling rapid and uniform staining of the strain and improving staining accuracy.

[0029] In this embodiment, the dividing circle in the standard bacterial area specifically has two symmetrically arranged overflow notches 30, forming a standard bacterial dividing line 31. The dividing circle in the sample bacterial area 13 specifically has six evenly distributed overflow notches 30, forming a sample bacterial dividing line 32. In the above structure, the standard bacterial dividing line 31 and the sample bacterial dividing line 32 are significantly distinguished by the different numbers and distributions of overflow notches 30, so that the experimenter can intuitively and clearly distinguish between the standard bacterial area and the sample bacterial area, further improving the ease of use.

[0030] Furthermore, the sample bacterial zone 13 includes two or more. In this embodiment, the sample bacterial zone 13 specifically includes two zones to facilitate parallel testing of the same strain or testing of two different strains.

[0031] Furthermore, both the dividing line 2 and the dividing circle are formed using epoxy paint. The chemical resistance, abrasion resistance, and durability of epoxy paint ensure the clarity of the dividing line 2 and the dividing circle in this embodiment, guaranteeing clear separation of the sample information marking area 11, the standard bacterial area, and the sample bacterial area 13. This ensures the dividing lines are not easily detached, allowing the slide to be reused after cleaning and sterilization. In addition, the structure achieved using epoxy paint is very simple, low-cost, and easy to implement, making it ideal for use as a disposable Gram staining slide.

Claims

1. A sterilized slide facilitating Gram staining and identification, comprising a slide main body (1) in a long strip shape, a sample information marking area (11), a standard bacteria area and a sample bacteria area (13) being arranged in sequence on the front surface of the slide main body (1), characterized in that: The sample information marking area (11), the standard bacteria area and the sample bacteria area (13) are sequentially arranged along the length direction of the slide main body (1), and are partitioned by the partition straight lines (2) coated on the front surface of the slide main body (1), the partition straight lines (2) are arranged along the width direction of the slide main body (1), and a plurality of partition straight lines (2) are sequentially and spacedly arranged along the length direction of the slide main body (1); the standard bacteria area and the sample bacteria area (13) are further respectively provided with partition circle lines; the thickness d of the partition straight lines (2) and the partition circle lines is 0.5-2 mm, and the width D is 0.5-2 mm.

2. The sterilized glass slide for ease of Gram staining and identification according to claim 1, characterized in that: The standard bacteria area includes two, namely a positive standard bacteria area (121) and a negative standard bacteria area (122).

3. The sterilized glass slide for ease of Gram staining and identification according to claim 2, wherein: The positive standard bacteria area (121) is provided with gram-positive bacteria in the partition circle line, and the negative standard bacteria area (122) is provided with gram-negative bacteria in the partition circle line.

4. The sterilized glass slide for ease of Gram staining and identification according to claim 1 or 2 or 3, characterized in that: The partition circle line has one overflow notch (30) or a plurality of uniformly distributed overflow notches (30).

5. The sterilized glass slide for ease of Gram staining and identification according to claim 4, wherein: The partition circle line in the standard bacteria area has two symmetrically arranged overflow notches (30), forming a standard bacteria partition line (31), and the partition circle line in the sample bacteria area (13) has 4-8 uniformly distributed overflow notches (30), forming a sample bacteria partition line (32).

6. The sterilized glass slide for ease of Gram staining and identification according to claim 1 or 2 or 3, characterized in that: The sample bacteria area (13) includes two or more than two.

7. The sterilized glass slide for ease of Gram staining and identification according to claim 1 or 2 or 3, characterized in that: The partition straight lines (2) and the partition circle lines are formed by epoxy paint.

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