A miniature microbial electrochemical test device and method suitable for space environment

Abstract

The application provides a miniature microbial electrochemical test device and method suitable for space environment, relates to the field of microbial electrochemical test equipment, and comprises a shell assembly, a reaction assembly, a liquid control assembly and an electrochemical detection assembly. The reaction assembly is arranged in the shell assembly, and the reaction assembly comprises a reaction cavity provided with a first connecting port. The liquid control assembly is arranged in the shell assembly and is in communication with the first connecting port. The liquid control assembly is used for injecting a microbial culture solution into the reaction cavity. The electrochemical detection assembly is arranged in the reaction cavity, the material of the working electrode is the sample to be tested, the test can be carried out in situ in the space environment, and the corrosion of the sample to be tested by the microorganism under the complex conditions in the space can be truly reflected. Through the integration scheme of the liquid control assembly and the reaction cavity, the microbial culture reaction can be carried out synchronously, and the strict requirements of the space environment on the space, quality and sample sealing performance can be met.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial electrochemical test equipment, and particularly relates to a miniature microbial electrochemical test device suitable for space environment. BACKGROUND

[0002] Microbial electrochemical analysis technology plays an important role in many microbial-related basic researches and practical applications such as material performance evaluation, environmental monitoring and ecological research, biological energy and conversion, drug screening and research and development, and the like, due to its high sensitivity, fast response and convenient operation.

[0003] There is a large amount of evidence showing that microorganisms in space environment are affected by various factors such as microgravity, cosmic radiation, vacuum and the like, and the biological characteristics will change obviously, in which the microgravity is the most important factor. At present, the related researches are mostly simulated by a ground rotating wall reactor or a three-dimensional gyroscope to simulate the microgravity effect, but the principle of the "simulated microgravity effect" is to disturb the perception of the biological body to the direction of gravity, and a real microgravity environment cannot be generated, so that real and effective data and information cannot be obtained. Meanwhile, the existing ground electrochemical research device has no special design in the structural design, especially in the integration and sealing, and cannot meet the technical requirements of the space environment on the scientific experiment load. SUMMARY

[0004] The present application provides a miniature microbial electrochemical test device suitable for space environment, to solve the problem that the existing ground electrochemical research device cannot meet the technical requirements of the space environment on the scientific experiment load with high integration and good sealing.

[0005] The present application provides a miniature microbial electrochemical test device suitable for space environment, comprising:

[0006] a shell assembly;

[0007] a reaction assembly arranged in the shell assembly, the reaction assembly comprising a reaction cavity, the reaction cavity being provided with a first connecting port;

[0008] a liquid control assembly arranged in the shell assembly, the liquid control assembly being in communication with the first connecting port, and the liquid control assembly being used for injecting a microbial culture solution into the reaction cavity;

[0009] an electrochemical detection assembly comprising a reference electrode, a working electrode and an auxiliary electrode, the reference electrode, the working electrode and the auxiliary electrode being arranged in the reaction cavity.

[0010] The present application provides a miniature microbial electrochemical test device suitable for space environment, the shell assembly comprising:

[0011] The main frame body;

[0012] The first shell is detachably connected with the main frame body, and the first shell cooperates with the main frame body to enclose a first cavity, and the reaction assembly and the liquid control assembly are arranged in the first cavity.

[0013] According to the embodiment of the present application, the shell assembly further comprises:

[0014] The second shell is detachably connected with the main frame body, and the second shell cooperates with the main frame body to enclose a second cavity.

[0015] According to the embodiment of the present application, the electrochemical detection assembly further comprises:

[0016] The fixing member is arranged in the second cavity, and the main frame body is internally provided with a through hole, and the reference electrode, the working electrode and the auxiliary electrode are all connected with the fixing member after penetrating through the through hole;

[0017] The sealing gasket is arranged between the fixing member and the main frame body, and the sealing gasket is used for sealingly cooperating with the fixing member and the main frame body.

[0018] According to the embodiment of the present application, the main frame body is internally hollow, the internal part of the main frame body is provided with a baffle, the through hole is located at the baffle, the reaction cavity is located at one side of the baffle, the fixing member is located at the other side of the baffle, and the sealing gasket is located at the side of the fixing member facing the baffle.

[0019] According to the embodiment of the present application, the lower part of the working electrode is in a cylindrical shape, and the top of the working electrode is in a circular plate shape; the lower part of the auxiliary electrode is in a cylindrical shape, and the top of the auxiliary electrode is in a circular ring shape, and the circular ring surrounds the outer periphery of the working electrode; the bottom of the reference electrode is in a cylindrical shape, and the outer surface of the top end of the reference electrode is provided with an outer shell, the material of the outer shell is a high-molecular polymer material, and the top end of the reference electrode is arranged outside the top circular ring of the auxiliary electrode.

[0020] According to the embodiment of the present application, the reaction assembly further comprises:

[0021] The heating member is arranged outside the reaction cavity, and the heating member is used for controlling the temperature inside the reaction cavity.

[0022] The liquid control assembly comprises:

[0023] The liquid storage bag is provided with a second connecting port and a gas return port,

[0024] The pump body is provided with a liquid inlet and a liquid outlet, the liquid inlet of the pump body is communicated with the second connecting port through a first pipeline, and the liquid outlet of the pump body is communicated with the first connecting port through a second pipeline.

[0025] The micro microbial electrochemical test device suitable for a space environment provided by the embodiment of the application further comprises:

[0026] The quick connector is electrically connected with the reaction assembly, the liquid control assembly and the electrochemical detection assembly through an electric connecting line.

[0027] The application further provides a micro microbial electrochemical test method suitable for a space environment, the method is based on the micro microbial electrochemical test device suitable for a space environment, and the method comprises the following steps:

[0028] The working electrode prepared from the sample to be tested is installed in the reaction cavity, microbial dry powder or a microsphere containing microorganisms is added into the reaction cavity in advance, and microbial culture solution is added into the liquid storage bag;

[0029] The first shell, the second shell and the main frame body are fixed;

[0030] The electrochemical test device is sent into a space environment by a spacecraft;

[0031] The liquid control assembly is controlled to inject the microbial culture solution into the reaction cavity, so that the microbial dry powder or the microsphere is activated, and a solution containing microorganisms is in contact with the sample to be tested;

[0032] The reference electrode, the working electrode and the auxiliary electrode are used to collect relevant electrochemical data.

[0033] The micro microbial electrochemical test device suitable for a space environment provided by the embodiment of the application integrates multiple functional components in a small structure through the integration scheme of the electrochemical detection assembly, the liquid control assembly and the reaction cavity, realizes high integration and sealing of each component, and is processed by high-strength and low-density metal or polymer material in combination with a surface treatment process, so that microbial culture and reaction can be simultaneously performed and have certain universality. The test device meets the strict requirements of space environment on space, mass, sample sealing property and structural strength, can carry out tests in a space in-situ environment, and truly reflects electrochemical information of microorganisms under space microgravity conditions. BRIEF DESCRIPTION OF DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the present application or the prior art, the drawings required to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort on the basis of these drawings.

[0035] Figure 1 is a schematic diagram of the three-dimensional structure of the micro microbial electrochemical test device suitable for space environment provided by the embodiment of the present application.

[0036] Figure 2 is an exploded structural schematic diagram of the micro microbial electrochemical test device suitable for space environment provided by the embodiment of the present application.

[0037] Figure 3 is a structural schematic diagram of the reference electrode, working electrode and auxiliary electrode provided by the embodiment of the present application.

[0038] Figure 4 is a schematic diagram of the assembly relationship of the reference electrode, working electrode and auxiliary electrode provided by the embodiment of the present application.

[0039] Figure 5 is a structural schematic diagram of the main frame body and the sealing gasket provided by the embodiment of the present application.

[0040] Figure 6 is a flow chart of the micro microbial electrochemical test method suitable for space environment provided by the embodiment of the present application.

[0041] Reference signs:

[0042] 10, housing assembly; 11, main frame body; 12, first housing; 13, second housing; 14, baffle; 15, circular boss; 20, reaction assembly; 21, reaction cavity; 22, first connecting port; 23, heating piece; 30, liquid control assembly; 31, liquid storage bag; 32, second connecting port; 33, gas return port; 34, pump body; 35, exhaust port; 40, electrochemical detection assembly; 41, reference electrode; 42, working electrode; 43, auxiliary electrode; 44, fixing piece; 45, sealing gasket; 50, quick connector. DETAILED DESCRIPTION

[0043] The embodiments of the present application will be further described in detail below in combination with the drawings and examples. The following examples are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0044] In the description of the embodiments of the present application, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0045] In the description of the embodiments of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0046] In the embodiments of the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0047] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present application and the features of different embodiments or examples without contradiction.

[0048] The specific structure of the micro microbial electrochemical test device suitable for space environment is described below. Figures 1-5 The specific structure of the micro microbial electrochemical test device suitable for space environment is described below.

[0049] As shown in Figures 1 to 2 The miniature microbial electrochemical test device suitable for space environment includes a shell assembly 10, a reaction assembly 20, a liquid control assembly 30 and an electrochemical detection assembly 40. The reaction assembly 20 is arranged in the shell assembly 10, and the reaction assembly 20 includes a reaction cavity 21 provided with a first connecting port 22. The liquid control assembly 30 is arranged in the shell assembly 10 and communicates with the first connecting port 22. The liquid control assembly 30 is used for injecting microbial culture solution into the reaction cavity 21. The electrochemical detection assembly 40 includes a reference electrode 41, a working electrode 42 and an auxiliary electrode 43, which are all arranged in the reaction cavity 21.

[0050] The miniature microbial electrochemical test device suitable for space environment provided by the embodiment of the present application can carry out tests in situ in space environment by arranging the electrochemical detection assembly 40 in the reaction cavity 21, and can truly reflect the electrochemical information of microorganisms under complex conditions in space. The microbial culture and corrosion reaction can be carried out synchronously by the integration scheme of the liquid control assembly 30 and the reaction cavity 21, which can meet the stringent requirements of space environment on space, quality and sample sealing.

[0051] In an embodiment of the present application, as shown in Figures 1 to 2 The reaction cavity 21 is in the form of a cylinder, and the inside of the reaction cavity 21 is hollow. The reaction cavity 21 provides a place for the miniature microbial electrochemical test, and the first connecting port 22 is located at the top of the reaction cavity 21. The material of the reaction cavity 21 is a corrosion-resistant polymer material, and of course, the material of the reaction cavity 21 can also be a metal material. When the material of the reaction cavity 21 is a metal material, a corrosion-resistant coating is arranged on the surface of the reaction cavity 21.

[0052] In an embodiment of the present application, as shown in Figure 2 The first connecting port 22 is used for connecting a second pipeline to inject microbial culture solution into the reaction cavity 21. Of course, when the size of the first connecting port 22 is large enough, the first connecting port 22 can be used to add microbial dry powder into the reaction cavity 21. Of course, a feeding port can also be arranged on the reaction cavity 21, and the feeding port is provided with a sealing cover. After the microbial dry powder is added, the sealing cover is closed to seal.

[0053] In an embodiment of the present application, the miniature microbial electrochemical test device suitable for space environment further includes a controller electrically connected with the reaction assembly 20, the liquid control assembly 30 and the electrochemical detection assembly 40. The controller is used for controlling the reaction assembly 20 and the liquid control assembly 30 to work and stop, and is also used for collecting relevant electrochemical data through the electrochemical detection assembly 40.

[0054] In an embodiment of the present application, as shown inFigures 1 to 2 As shown in the figure, the shell assembly 10 comprises a main frame 11 and a first shell 12, the first shell 12 is a cylindrical shell, the lower end of the first shell 12 is provided with an opening, the first shell 12 is detachably connected with the main frame 11, specifically, the inner edge of the opening of the first shell 12 is provided with internal threads, the outer circumferential surface of the upper end of the main frame 11 is provided with external threads, the upper end of the first shell 12 is screwed with the main frame 11, the first shell 12 and the main frame 11 enclose a first cavity, by using screw connection, the installation and dismounting of the first shell 12 can be facilitated. Of course, the connection mode of the first shell 12 and the main frame 11 is not limited to screw connection, but also can use buckles, pins or other connection modes.

[0055] The reaction assembly 20 and the liquid control assembly 30 are both arranged in the first cavity, the liquid control assembly 30 is located above the reaction assembly 20, so as to facilitate the injection of the microbial culture solution in the liquid storage bag 31 into the reaction cavity 21 under the action of the pump body 34.

[0056] In an embodiment of the present application, as shown in the figure, Figures 1 to 2 The shell assembly 10 further comprises a second shell 13, the second shell 13 is a cylindrical shell, the upper end of the second shell 13 is provided with an opening, the second shell 13 is detachably connected with the main frame 11, specifically, the inner edge of the opening of the second shell 13 is provided with internal threads, the outer circumferential surface of the lower end of the main frame 11 is provided with external threads, the lower end of the second shell 13 is screwed with the main frame 11, the second shell 13 and the main frame 11 enclose a second cavity, by using screw connection, the installation and dismounting of the second shell 13 can be facilitated. Of course, the connection mode of the second shell 13 and the main frame 11 is not limited to screw connection, but also can use buckles, pins or other connection modes.

[0057] In a specific embodiment of the present application, the material of the main frame 11, the first shell 12 and the second shell 13 is polyimide or other high polymer plastic material, using the above-mentioned material effectively enhances the corrosion resistance of the shell assembly 10.

[0058] In an embodiment of the present application, as shown in the figure, Figure 5 The electrochemical detection assembly 40 further comprises a fixing member 44 and a sealing gasket 45, the fixing member 44 is arranged in the second cavity, the fixing member 44 is used to provide a mounting basis for the reference electrode 41, the working electrode 42 and the auxiliary electrode 43, the inside of the main frame 11 is provided with a through hole, the reference electrode 41, the working electrode 42 and the auxiliary electrode 43 are all connected with the fixing member 44 after penetrating through the through hole;

[0059] The sealing gasket 45 is arranged between the fixing member 44 and the main frame 11, the sealing gasket 45 is used to seal with the fixing member 44 and the main frame 11.

[0060] In one embodiment of the present application, as shown in Figure 4 The fixing member 44 is a circular disc, and the shape of the fixing member 44 is not limited to this, but can also be a circular truncated cone or other shapes. The outer diameter of the fixing member 44 is smaller than the inner diameter of the main frame 11. The fixing member 44 is provided with a circular boss 15 on the side facing the reaction cavity 21. The bottom of the reaction cavity 21 is provided with a mounting hole. After the circular boss 15 passes through the through hole, it is embedded in the mounting hole and sealingly fits in the mounting hole. Three connecting through holes are arranged at intervals on the circular boss 15. The lower ends of the reference electrode 41, the working electrode 42 and the auxiliary electrode 43 are respectively inserted into the three connecting through holes.

[0061] In one embodiment of the present application, as shown in Figure 2 The micro-microbial electrochemical test device suitable for space environment also includes a quick connector 50. The quick connector 50 is electrically connected to the reaction assembly 20, the liquid control assembly 30 and the electrochemical detection assembly 40 through the electric connecting lines. The use of the quick connector 50 shortens the connection time between the electrochemical test device and the external controller, and improves the connection reliability and sealing between the electrochemical test device and the external controller.

[0062] Preferably, the quick connector 50 is a six-core aviation plug. The quick connector 50 is installed at the bottom of the second shell 13. The quick connector 50 is used to connect with the controller, so as to realize the electrical connection between the controller and the reaction assembly 20, the liquid control assembly 30 and the electrochemical detection assembly 40. Of course, the specific type of the quick connector 50 is not limited to the six-core aviation plug, and other types of quick connectors can also be used. When installed, the six-core aviation plug is electrically connected to the external controller. The controller is electrically connected to the external electrochemical workstation for data reading and device control.

[0063] In one embodiment of the present application, as shown in Figure 5 The inside of the main frame 11 is hollow. The inside of the main frame 11 is provided with a baffle 14. The baffle 14 is a circular ring. A through hole is located at the center of the baffle 14. The through hole is used to pass through the reference electrode 41, the working electrode 42 and the auxiliary electrode 43. The baffle 14 divides the inside of the main frame 11 into two parts. The reaction cavity 21 is located on one side of the baffle 14, i.e. on the upper side of the baffle 14. The fixing member 44 is located on the other side of the baffle 14, i.e. on the lower side of the baffle 14. The sealing gasket 45 is located on the side of the fixing member 44 facing the baffle 14, i.e. on the upper side of the fixing member 44. After the fixing member 44 is assembled with the reaction cavity 21, the fixing member 44 cooperates with the baffle 14 to extrude the sealing gasket 45, so as to realize the sealing fit between the fixing member 44 and the baffle 14, and prevent the liquid from entering the second cavity after the mounting hole at the bottom of the reaction cavity leaks, which affects the normal work of the circuit.

[0064] In one embodiment of the present application, as shown in Figures 3 to 4 The reference electrode 41 is in a rod structure, and is made of metal. Preferably, the reference electrode 41 is made of metal Ag or AgCl. An outer surface of the reference electrode 41 is provided with an outer shell made of a high polymer material to avoid damage of the reference electrode 41 during launching of the spacecraft.

[0065] The working electrode 42 is made of a sample to be tested. A top portion of the working electrode 42 is in a circular plate shape. The sample to be tested can be selected according to a test design. For example, for a corrosion test, the sample can be designed as various aerospace materials, including but not limited to metal materials with or without a coating. The metal material can be stainless steel, titanium alloy, aluminum alloy, magnesium alloy, or other metal materials. The coating can include but is not limited to an antibacterial coating, an oxide film, a DLC-based coating, a MoS2-based coating, a CrN-based coating, a TiN-based coating, and a TiO2-based coating.

[0066] A lower portion of the auxiliary electrode 43 is in a cylindrical shape, and a top portion of the auxiliary electrode 43 is in a circular ring shape. The circular ring surrounds an outer periphery of the working electrode 42, and the working electrode 42 is arranged in the circular ring of the auxiliary electrode 43. The space utilization is improved as much as possible while ensuring that the auxiliary electrode 43 and the working electrode 42 have a sufficient area ratio. The auxiliary electrode 43 can be made of Pb, graphite, metal oxide, or other materials. A bottom portion of the reference electrode 41 is in a cylindrical shape, and an outer surface of a top end of the reference electrode 41 is provided with an outer shell made of a high polymer material. The high polymer material can protect an internal electrode wire from sliding during launching of the spacecraft. The top end of the reference electrode 41 is arranged outside the circular ring of the top portion of the auxiliary electrode 43. Adjacent two of the reference electrode 41, the working electrode 42, and the auxiliary electrode 43 are spaced apart by a certain distance. The reference electrode 41, the working electrode 42, and the auxiliary electrode 43 are electrically connected to the quick connector 50 through electrical connection lines. The lower ends of the reference electrode 41, the working electrode 42, and the auxiliary electrode 43 are connected to the fixing member 44, and thus are integrally arranged on the main frame body 11.

[0067] In one embodiment of the present application, as shown in Figure 2 The reaction assembly 20 further includes a heating member 23 arranged outside the reaction cavity 21. The heating member 23 is used to control the temperature inside the reaction cavity 21. Preferably, the heating member 23 is a heating film. The heating film is attached to an outer peripheral surface of the reaction cavity 21. Preferably, the heating film is in a ring shape and is sleeved on the outer peripheral surface of the reaction cavity 21. The heating film is made of a polyimide material. The heating member 23 is electrically connected to the quick connector 50 through an electrical connection line. The temperature of the heating member 23 can be controlled by an external controller, and thus the temperature inside the reaction cavity 21 can be controlled.

[0068] In one embodiment of the present application, as shown inFigure 2 As shown, the liquid control assembly 30 includes a reservoir 31 and a pump body 34. The reservoir 31 is provided with a second connection port 32 and a return air port 33. Specifically, the reservoir 31 is located above the pump body 34, and the second connection port 32 and the return air port 33 are located at the bottom of the reservoir 31.

[0069] The pump body 34 is a miniature peristaltic pump. The pump body 34 is electrically connected to the quick connector 50. The inlet of the pump body 34 is connected to the second connection port 32 through the first pipeline, and the outlet of the pump body 34 is connected to the first connection port 22 through the second pipeline. The reaction chamber 21 is provided with an exhaust port 35, which is connected to the return gas port 33 through the return gas pipeline.

[0070] Since the reaction chamber 21 is in a closed state, when the controller controls the pump body 34 to inject the microbial culture solution into the reaction chamber 21, the internal pressure of the reaction chamber 21 increases, preventing subsequent liquid from entering normally. Therefore, a return air pipeline is used to connect the exhaust port 35 and the return air port 33, allowing the air in the reaction chamber 21 to enter the liquid storage bladder 31 through the return air pipeline. This ensures that the liquid can smoothly enter the reaction chamber 21 while preventing the liquid storage bladder 31 from being crushed due to liquid discharge.

[0071] The working principle of the microbial electrochemical experimental device for space environment of the present invention:

[0072] Before the experiment, the quick connector 50 was electrically connected to the controller, and the controller was electrically connected to the multi-channel electrochemical workstation. During the experiment, the changing trends of electrochemical parameters in microbial metabolic activities were measured using the reference electrode 41, working electrode 42, and auxiliary electrode 43. These included calculating the content of small molecule organic acid metabolites by measuring the conductivity of the culture medium; assessing microbial viability by measuring the redox potential of the culture medium; and evaluating the catalytic activity of microorganisms on the redox reactions of H+, ROH, and other particles in the culture medium by measuring charge transfer resistance using electrochemical impedance spectroscopy.

[0073] like Figure 6 As shown, the present invention also provides a microbial electrochemical testing method suitable for space environments. The method is based on the microbial electrochemical testing device suitable for space environments described in any of the above embodiments. The microbial electrochemical testing method suitable for space environments includes:

[0074] Step 100: Install the working electrode 42 made from the sample to be tested into the reaction chamber 21, add microbial dry powder into the reaction chamber 21, and add microbial culture solution into the liquid control component 30;

[0075] Before the experiment starts, the sample to be tested is made into the working electrode 42, the working electrode 42, the working electrode 42 and the auxiliary electrode 43 are installed in the reaction cavity 21, after the electrode installation is completed, the microbial dry powder or the microsphere containing the microorganism is pre-added to the reaction cavity 21, the microbial culture solution is added to the liquid control assembly 30, that is, the microbial culture solution is added to the liquid storage bag 31 for subsequent reaction, and the amount of the microbial culture solution needs to be greater than the subsequent use amount.

[0076] Step 200, fix the first shell 12, the second shell 13 and the main frame body;

[0077] Step 300, send the electrochemical test device into the space environment by the spacecraft;

[0078] Before step 300 is performed, the components of the test device need to be assembled, that is, the first shell 12 is connected with the main frame body 11, the second shell 13 is connected with the main frame body 11, and the quick connector 50 is connected with the external controller.

[0079] Step 400, control the liquid control assembly 30 to inject the microbial culture solution into the reaction cavity 21, so as to activate the microbial dry powder or the microsphere, and make the solution containing the microorganism contact with the sample to be tested;

[0080] After reaching the space environment, the controller controls the pump body 34 to work, and the microbial culture solution is pumped into the reaction cavity 21 by the pump body 34, and since the flow of the pump body 34 is constant, the volume of the injected microbial culture solution can be controlled according to time.

[0081] In a preferred embodiment of the present application, before the step of controlling the liquid control assembly 30 to inject the microbial culture solution into the reaction cavity 21 is performed, the step of controlling the heating element 23 to work to heat the reaction cavity 21 to a predetermined temperature also needs to be performed; in the present embodiment, the predetermined temperature is 27℃, of course, the predetermined temperature can also be 26℃, 28℃ or other temperatures; by making the temperature inside the reaction cavity 21 constant, the accuracy of the test results can be ensured.

[0082] Step 500, collect relevant electrochemical data through the reference electrode 41, the working electrode 42 and the auxiliary electrode 43.

[0083] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A miniature microbial electrochemical test device suitable for use in space environments, characterized in that, The utility model relates to a portable electrochemical detection device for microorganism culture solution, which comprises the following components: A shell assembly (10); A reaction assembly (20) arranged in the shell assembly (10), wherein the reaction assembly (20) comprises a reaction cavity (21) provided with a first connecting port (22); A liquid control assembly (30) arranged in the shell assembly (10), wherein the liquid control assembly (30) is in communication with the first connecting port (22), and the liquid control assembly (30) is used for injecting a microorganism culture solution into the reaction cavity (21); An electrochemical detection assembly (40) comprising a reference electrode (41), a working electrode (42) and an auxiliary electrode (43), wherein the reference electrode (41), the working electrode (42) and the auxiliary electrode (43) are all arranged in the reaction cavity (21). The shell assembly (10) comprises: A main frame (11); A first shell (12) detachably connected with the main frame (11), wherein the first shell (12) cooperates with the main frame (11) to enclose a first cavity, and the reaction assembly (20) and the liquid control assembly (30) are both arranged in the first cavity; The shell assembly (10) further comprises: A second shell (13) detachably connected with the main frame (11), wherein the second shell (13) cooperates with the main frame (11) to enclose a second cavity; The electrochemical detection assembly (40) further comprises: A fixing member (44) arranged in the second cavity, wherein the main frame (11) is internally provided with a through hole, and the reference electrode (41), the working electrode (42) and the auxiliary electrode (43) are all connected with the fixing member (44) after passing through the through hole; A sealing gasket (45) arranged between the fixing member (44) and the main frame (11), wherein the sealing gasket (45) is used for sealingly cooperating with the fixing member (44) and the main frame (11); The liquid control assembly (30) comprises: A liquid storage bag (31) provided with a second connecting port (32) and a gas return port (33), A pump body (34), wherein a liquid inlet of the pump body (34) is in communication with the second connecting port (32) through a first pipeline, a liquid outlet of the pump body (34) is in communication with the first connecting port (22) through a second pipeline, and the reaction cavity (21) is provided with an exhaust port (35) in communication with the gas return port (33) through a gas return pipeline.

2. The micro-microbe electrochemical test device suitable for space environment according to claim 1, characterized in that, The main frame (11) is internally hollow, the main frame (11) is internally provided with a baffle (14), the through hole is located on one side of the baffle (14), the fixing member (44) is located on the other side of the baffle (14), and the sealing gasket (45) is located on the side of the fixing member (44) facing the baffle (14).

3. The micro-microbial electrochemical test device suitable for space environment according to claim 1 or 2, characterized in that, The lower part of the working electrode (42) is cylindrical, and the top of the working electrode (42) is a round piece; the lower part of the auxiliary electrode (43) is cylindrical, and the top of the auxiliary electrode (43) is a round ring, which surrounds the outer periphery of the working electrode (42); the bottom of the reference electrode (41) is cylindrical, and the outer surface of the top end of the reference electrode (41) is provided with a shell, the material of the shell is a high polymer polymeric material, and the top end of the reference electrode (41) is arranged outside the top ring of the auxiliary electrode (43).

4. The micro-microbe electrochemical test device suitable for space environment according to claim 1 or 2, characterized in that, The reaction assembly (20) further comprises: A heating element (23) is arranged outside the reaction cavity (21), and the heating element (23) is used to control the temperature inside the reaction cavity (21).

5. The micro-microbial electrochemical test device suitable for space environment according to claim 1 or 2, characterized in that, Further comprising: A quick connector (50) is electrically connected to the reaction assembly (20), the liquid control assembly (30) and the electrochemical detection assembly (40) through an electric connection line.

6. A micro-microbial electrochemical test method suitable for space environment, based on the micro-microbial electrochemical test device suitable for space environment according to any one of claims 1 to 5, characterized in that, The method comprises: The working electrode (42) made of the sample to be tested is installed in the reaction cavity (21), and microbial dry powder or microspheres containing microorganisms are pre-added into the reaction cavity (21), and microbial culture solution is added into the liquid storage bag (31); The first shell (12), the second shell (13) and the main frame (11) are fixed; The electrochemical test device is sent into the space environment by a spacecraft; The liquid control assembly (30) is controlled to inject the microbial culture solution into the reaction cavity (21) to activate the microbial dry powder or the microspheres, so that the solution containing microorganisms is in contact with the sample to be tested; The relevant electrochemical data are collected by the reference electrode (41), the working electrode (42) and the auxiliary electrode (43).

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