Quantitative mixing structure and microfluidic chip

Abstract

The present application relates to a quantitative mixing structure and microfluidic chip. The quantitative mixing structure includes a main chamber and a vent valve. The main chamber is provided with an inlet for sample entry. The main chamber is used to allow the sample liquid level to rise and fill along a reference direction. The vent valve is connected to the main chamber, and along the reference direction, the connection between the vent valve and the main chamber is spaced from the inlet and the bottom side of the main chamber. The vent valve allows gas to flow out of the main chamber and blocks liquid from flowing out of the main chamber. When the liquid level rises to submerge the connection between the vent valve and the main chamber, the liquid blocks the vent valve, limiting the pressure relief of the main chamber, thereby achieving sample quantification. Furthermore, when the sample is immersed in the vent valve but has not yet risen to the inlet, a certain amount of space remains in the main chamber for the sample to undergo a mixing reaction. In this way, sample quantification and mixing can be completed simultaneously within the main chamber, simplifying the structure of the quantitative mixing structure. The microfluidic chip including the above-mentioned quantitative mixing structure facilitates miniaturization.

Description

Technical Field

[0001] The present application relates to the technical field of microfluidic chips, and in particular to a quantitative mixing structure and a microfluidic chip. Background Art

[0002] A microfluidic chip is a microanalysis system that integrates operational units such as sample pretreatment, mixing, reaction, separation, and detection. Microfluidic chips require minimal sample volume, are simple to operate, and can accurately complete the entire process from sample preparation to result display in a short period of time. This effectively overcomes the experimental errors often associated with manual labor in traditional laboratory work.

[0003] In traditional technology, when conducting reaction tests on microfluidic chips, it is usually necessary to quantify the reagents in one chamber and transfer the quantified reagents to another chamber for mixing. This structural arrangement is not conducive to further miniaturization of microfluidic chips. Summary of the Invention

[0004] Based on this, it is necessary to provide a quantitative mixing structure and a microfluidic chip in order to simplify the quantitative structure and mixing structure of the microfluidic chip.

[0005] On the one hand, the present application provides a quantitative mixing structure, which includes a main chamber and a breathable valve. The main chamber is provided with an inlet for allowing a sample to enter, and the main chamber is used to allow the liquid level of the sample to rise and fill along a reference direction; the breathable valve is connected to the main chamber, and along the reference direction, the connection between the breathable valve and the main chamber is spaced apart from the inlet and the bottom side of the main chamber; the breathable valve allows gas to flow out of the main chamber and blocks liquid from flowing out of the main chamber.

[0006] In one embodiment, the main chamber includes a bottom and a circumferential portion connected to the periphery of the bottom. The bottom and the circumferential portion together form the cavity of the main chamber and are used for the liquid level of the sample to rise from the bottom along the circumferential portion to fill. The circumferential portion is provided with a connecting port connected to the air valve. Along the reference direction, the connecting port is spaced apart from the sampling port and the bottom.

[0007] In one embodiment, the annular portion includes a neck, which is retracted into the main chamber relative to other areas of the annular portion, and the shortest distance from each area of ​​the plane where the neck is located to the plane where the bottom is located is equal; the communication port is opened in the neck.

[0008] In one embodiment, the neck divides the space inside the main chamber into a mixing chamber and a quantitative chamber. The mixing chamber and the quantitative chamber are respectively located on both sides of the neck and are connected to each other. The injection port is opened on the side where the mixing chamber is located. The cross-sectional size of the mixing chamber gradually decreases along the direction approaching the neck.

[0009] In one embodiment, the quantitative cavity includes a first constriction area relatively close to the neck, and the cross-sectional size of the first constriction area gradually decreases in a direction close to the neck.

[0010] In one embodiment, the quantitative mixing structure further includes a shut-off valve and a waste liquid chamber. The shut-off valve is provided at the bottom and connected between the main chamber and the waste liquid chamber. The shut-off valve is used to connect or cut off the main chamber and the waste liquid chamber.

[0011] In one embodiment, the quantitative chamber includes a second closing area relatively close to the waste liquid chamber, and the cross-sectional size of the second closing area gradually decreases in the direction close to the waste liquid chamber.

[0012] On the other hand, the present application further provides a microfluidic chip, which includes the quantitative mixing structure as described above.

[0013] In one embodiment, the microfluidic chip further includes an injection channel, which is connected to the injection port and is used for allowing the sample to flow into the main chamber; a one-way valve is provided in the injection channel, and the one-way valve is used for allowing the sample to flow in one direction to the injection port.

[0014] In one embodiment, the quantitative mixing structure also includes a sample inlet channel connected to the sample inlet, and the microfluidic chip includes multiple quantitative mixing structures, each of the sample inlet channels is connected to the sample inlet channel in parallel; each of the sample inlet channels is provided with a switch valve, and the switch valve is used to open or cut off the sample inlet channel.

[0015] In the aforementioned quantitative mixing structure, the vent valve is spaced apart from the bottom of the main chamber in the direction of rising liquid level (i.e., the reference direction). The vent valve allows gas flow while blocking liquid flow. Therefore, when the liquid level has not yet reached the connection between the vent valve and the main chamber, the main chamber can release pressure through the vent valve. When the liquid level rises to submerge the connection between the vent valve and the main chamber, the liquid blocks the vent valve, restricting pressure release in the main chamber. At this point, the sample cannot continue to enter the main chamber due to the pressure within the main chamber, achieving quantitative sample quantification. Furthermore, the vent valve is spaced apart from the sample inlet in the direction of rising liquid level. This means that when the sample is submerged at the vent valve, it has not yet risen to the sample inlet, leaving sufficient space within the main chamber for sample mixing. This arrangement allows for simultaneous sample quantification and mixing within the main chamber, simplifying the overall structure of the quantitative mixing structure and the microfluidic chip incorporating it, facilitating miniaturized microfluidic chip design. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of a quantitative mixing structure provided in one embodiment of the present application.

[0017] Figure 2 for Figure 1 The schematic diagram of the quantitative mixing structure shown is when the liquid level has not reached the connection point between the air valve and the main chamber.

[0018] Figure 3 for Figure 1 The schematic diagram of the quantitative mixing structure shown is when the liquid level is immersed in the connection between the air valve and the main chamber.

[0019] Figure 4 A schematic diagram of the shape of the main chamber provided for the second aspect of an embodiment of the present application.

[0020] Figure 5 A schematic diagram of the shape of the main chamber provided for the third aspect of an embodiment of the present application.

[0021] Figure 6 A schematic diagram of the shape of the main chamber provided for the fourth aspect of an embodiment of the present application.

[0022] Figure 7 This is a simplified schematic diagram of the modules of the microfluidic chip provided in one embodiment of the present application.

[0023] Figure numerals: 10, microfluidic chip; 11, quantitative mixing structure; 12, sample inlet channel; 13, one-way valve; 100, main chamber; 101, sample inlet; 102, peripheral part; 103, bottom; 104, neck; 105, connecting port; 110, mixing chamber; 120, quantitative chamber; 121, first closing area; 122, second closing area; 200, air valve; 300, stop valve; 400, waste liquid chamber; 500, sample inlet channel; 600, switch valve; K, reference direction. DETAILED DESCRIPTION

[0024] To make the above-mentioned objects, features, and advantages of the present application more clearly understood, the specific embodiments of the present application are described in detail below with reference to the accompanying drawings. The following description sets forth many specific details to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways than those described herein, and those skilled in the art can make similar improvements without violating the scope of the present application. Therefore, the present application is not limited to the specific embodiments disclosed below.

[0025] In the description of this application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0026] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, if the term "plurality" appears, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.

[0027] In this application, unless otherwise specified or limited, the terms "mounted," "connected," "connected," "fixed," etc., should be interpreted broadly. For example, these terms may refer to fixed connections, removable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediary; and internal communication between two components or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0028] In this application, unless otherwise expressly specified or limited, if a first feature is described as being "above" or "below" a second feature, or similar descriptions, this may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is described as being "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is described as being "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0029] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only embodiment.

[0030] See Figure 1 , Figure 1 A schematic diagram of a quantitative mixing structure according to an embodiment of the present application is shown. The quantitative mixing structure 11 provided in one embodiment of the present application includes a main chamber 100 and a vent valve 200. The main chamber 100 is provided with an inlet 101 for admitting a sample. The main chamber 100 is configured to allow the sample liquid level to rise and fill along a reference direction K. The vent valve 200 is in communication with the main chamber 100. Along the reference direction K, the connection between the vent valve 200 and the main chamber 100 is spaced apart from the inlet 101 and the bottom side of the main chamber 100. The vent valve 200 allows gas to flow out of the main chamber 100 and blocks liquid from flowing out of the main chamber 100.

[0031] In the quantitative mixing structure 11 described above, the air valve 200 is spaced apart from the bottom of the main chamber 100 in the direction of rising liquid level (i.e., reference direction K). The air valve 200 allows gas flow but blocks liquid flow. Therefore, when the liquid level does not reach the connection between the air valve 200 and the main chamber 100, the main chamber 100 can release pressure through the air valve 200. When the liquid level rises to submerge the connection between the air valve 200 and the main chamber 100, the liquid blocks the air valve 200, restricting pressure release from the main chamber 100. At this point, the sample is unable to enter the main chamber 100 due to the pressure within the main chamber 100, achieving quantitative determination of the sample. Furthermore, in the direction of rising liquid level, the vent valve 200 is spaced apart from the sample inlet 101. This means that when the sample is submerged at the vent valve 200, it has not yet risen to the sample inlet 101. This means that there is still sufficient space within the main chamber 100 for sample mixing. This arrangement allows for simultaneous quantification and mixing of the sample within the main chamber 100, simplifying the overall structure of the quantification and mixing structure 11 and the microfluidic chip incorporating it, facilitating miniaturization of the microfluidic chip.

[0032] It is easy to understand, see Figure 2 As the sample gradually enters the main chamber 100, when the sample liquid level has not yet risen to the breathable valve 200, as shown by arrow Q, the gas in the main chamber 100 can flow out of the main chamber 100 through the breathable valve 200, causing the main chamber 100 to release pressure. As a result, the sample entering the main chamber 100 from the injection port 101 and the gas discharged from the main chamber 100 from the breathable valve 200 can form a dynamic balance, and the sample can continue to enter the main chamber 100 smoothly. Figure 3 When the sample liquid level submerges the vent valve 200, the vent valve 200 blocks the outflow of liquid, while the liquid submerging into the connection further blocks the outflow of gas from the vent valve 200. Consequently, the main chamber 100 cannot continue to release pressure through the vent valve 200. The sample is restricted by the pressure inside the main chamber 100 and cannot continue to enter the main chamber 100, thus achieving sample quantification.

[0033] The breathable valve 200, also known as a waterproof or hydrophobic breathable valve, is a sealed mounting component formed by combining a waterproof breathable membrane with other materials such as plastic, metal, or silicone through injection molding or ultrasonic welding. The waterproof breathable membrane, also known as breathing paper, is a polymer waterproof material. Due to the large spacing between gas molecules, they can diffuse through the membrane's pores. Liquid molecules, however, are spaced smaller than the pores, and surface tension prevents them from passing through the membrane, effectively providing a waterproof effect.

[0034] In this application, once the sample liquid level reaches the connection between the vent valve 200 and the main chamber 100, the sample can no longer enter the main chamber 100. Consequently, along reference direction K, the volume of the cavity between the connection and the bottom of the main chamber 100 (i.e., the quantitative chamber 120, referred to below) is the quantitative volume of the sample. In other words, by adaptively adjusting the volume of this portion of the cavity, the desired sample volume can be achieved, enabling controllable quantitative sample collection.

[0035] It should be noted that when the main chamber 100 is placed vertically in the direction of gravity, the sample will be loaded into the main chamber 100 along the direction of gravity, that is, the reference direction K coincides with the direction of gravity. In this case, the bottom side of the main chamber 100 is the bottom side in the direction of gravity.

[0036] Furthermore, in each embodiment of the present application, the breathable valve 200 is used in conjunction with the main chamber 100 to achieve quantitative and uniform mixing of the sample, and there is no specific requirement for the shape of the main chamber 100. Figure 1 As shown, when the main chamber 100 is roughly cylindrical, the bottom side can be the bottom wall of the cylindrical main chamber 100 (i.e., the bottom 103 mentioned below). At this time, the air valve 200 can be arranged on the circumferential wall of the main chamber 100 (i.e., the circumferential portion 102 mentioned below) to achieve sample quantification.

[0037] For example, Figure 4 As shown, when the main chamber 100 is spherical as a whole, the bottom side of the main chamber 100 can be one side in the reference direction K (such as Figure 4 As shown in S1 in FIG, the connection point between the vent valve 200 and the main chamber 100 can be arranged at a position with a certain distance from the bottom side in the reference direction K (as shown in FIG. Figure 4 (as shown in P1).

[0038] For example, Figure 5 As shown, when the main chamber 100 is in the shape of an inverted cone or an inverted pyramid, the bottom side of the main chamber 100 may be located at the apex of the cone or pyramid (e.g., Figure 5 At this time, the connection point between the vent valve 200 and the main chamber 100 can be arranged at a position with a certain distance from the bottom side in the reference direction K (as shown in S2). Figure 5 (as shown in P2).

[0039] For example, Figure 6 As shown, when the main chamber 100 is spindle-shaped, the bottom side of the main chamber 100 may be located at the vertex of one side of the spindle-shaped main chamber 100 (as shown in FIG. Figure 6 At this time, the connection point between the vent valve 200 and the main chamber 100 can be arranged at a position with a certain distance from the bottom side in the reference direction K (as shown in S3). Figure 6(as shown in P3, P4 and P5).

[0040] That is to say, the bottom side of the main chamber 100 is not limited to being a flat bottom surface, and the above-mentioned vertex can also serve as the bottom side of the main chamber 100. It should be noted that although the present application does not specifically limit the shape of the main chamber 100, for ease of understanding and description, the following still uses the shape of the main chamber 100 as the bottom side. Figures 1 to 3 The shape of the main chamber 100 shown is described as an example.

[0041] Please refer again Figure 1 In one embodiment, the main chamber 100 includes a bottom 103 and an annular portion 102 connected to the periphery of the bottom 103. The bottom 103 and the annular portion 102 together form the cavity of the main chamber 100, and are used for the sample liquid to rise from the bottom 103 along the annular portion 102 and fill. The annular portion 102 defines a communication port 105 that communicates with the breathable valve 200. The communication port 105 is spaced apart from the injection port 101 and the bottom 103 along the reference direction K. In this case, the volume of the cavity between the communication port 105 and the bottom 103 in the main chamber 100 along the reference direction K is sufficient to quantify the sample.

[0042] Please continue reading Figure 1 In one embodiment, the annular portion 102 includes a neck 104, and the neck 104 retracts into the main chamber 100 relative to other areas of the annular portion 102. That is, the neck 104 has a smaller cross-sectional size relative to other parts of the annular portion 102. The cross-sectional size described in each embodiment of the present application refers to the size of the cross section perpendicular to the reference direction K. The shortest distance from each area of ​​the plane where the neck 104 is located to the plane where the bottom 103 is located is equal. The connecting port 105 is opened in the neck 104, that is, the air valve 200 is connected to the main chamber 100 at the neck 104, and the quantitative input of the sample is completed when the liquid level rises to the neck 104. In other words, the quantitative input of the sample is completed when the liquid level rises to the part with a smaller cross-section in the main chamber 100, which can improve the accuracy of sample quantification and reduce quantitative errors.

[0043] It is easy to understand that when the liquid level rises to the communication port 105, the sample is fully quantitatively input. However, when the sample is fully quantitatively input, it is inevitable that there will be errors in the liquid level height in the reference direction K within a certain range. In this embodiment, since the liquid level is located at the neck portion 104, which has a smaller cross-sectional dimension, when the sample is fully quantitatively input, the amount of sample per unit height at the neck portion 104 is relatively small, which reduces the error range for the sample quantitative input. From another perspective, for cylindrical containers of the same height, the larger the inner diameter, the greater the volume. Therefore, when the liquid level rises to the same height, the change in the sample amount is smaller when the cross-sectional dimension is smaller, resulting in a smaller error.

[0044] Please continue reading Figure 1In one embodiment, the neck 104 divides the space within the main chamber 100 into a mixing chamber 110 and a quantitative chamber 120. The mixing chamber 110 and the quantitative chamber 120 are located on either side of the neck 104 and are interconnected. The sample inlet 101 is located on the side where the mixing chamber 110 is located, and the sample can be loaded into the quantitative chamber 120 through the mixing chamber 110. As it approaches the neck 104, the cross-sectional size of the mixing chamber 110 gradually decreases, approximating a curved or conical surface, to facilitate guiding the sample into the quantitative chamber 120 and reduce the probability of large quantitative errors caused by the sample residing in the mixing chamber 110.

[0045] Please continue reading Figure 1 In one embodiment, the metering chamber 120 includes a first constricted area 121 and a second constricted area 122. The first constricted area 121 is relatively close to the neck 104. As it approaches the neck 104, the cross-sectional dimensions of the first constricted area 121 gradually decrease, approximating a curved or conical surface. Thus, within a certain range near the neck 104, the main chamber 100 has a relatively small cross-sectional dimension, reducing metering errors.

[0046] Furthermore, the quantitative mixing structure 11 also includes a shut-off valve 300 and a waste liquid chamber 400. The shut-off valve 300 is provided at the bottom 103 and communicates between the main chamber 100 and the waste liquid chamber 400. The shut-off valve 300 is used to connect or disconnect the main chamber 100 and the waste liquid chamber 400. When filling and mixing the sample, the main chamber 100 can be disconnected to make the main chamber 100 relatively closed. After the reaction is completed, the main chamber 100 can be opened to allow the waste liquid in the main chamber 100 to flow into the waste liquid chamber 400, so that the main chamber 100 can carry out the next mixing reaction.

[0047] Furthermore, the second closing area 122 is relatively close to the waste liquid chamber 400 , and along the direction approaching the waste liquid chamber 400 , the cross-sectional size of the second closing area 122 gradually decreases and approximates an arc surface or a conical surface, so as to facilitate the complete discharge of waste liquid from the main chamber 100 .

[0048] In one embodiment, the mixing chamber 110 may be pre-filled with sample reagents to be reacted, such as one or more of a solid reagent, a freeze-dried reagent, a liquid reagent, a solid-phase reagent adsorbed on the chamber, and the like.

[0049] Furthermore, the vent valve 200 is provided with a channel, which can be in different shapes such as straight line, broken line, etc., and the depth can be 0.01mm-0.5mm. The vent valve 200 and the stop valve 300 can also be provided with other hydraulic components with corresponding functions.

[0050] Furthermore, the injection port 101 may be circular, elliptical, trumpet-shaped, square, rectangular or other shapes.

[0051] See also Figure 7 An embodiment of the present application further provides a microfluidic chip 10. The microfluidic chip 10 includes the quantitative mixing structure 11, so the microfluidic chip 10 can have a more compact and smaller structure.

[0052] See also Figure 1 and Figure 7 In one embodiment, the microfluidic chip 10 further includes an inlet channel 12, which is connected to the inlet port 101 and is used to allow the sample to flow into the main chamber 100. A one-way valve 13 is provided in the inlet channel 12. The one-way valve 13 is used to ensure one-way flow of the sample to the inlet port 101 to prevent backflow of the sample during mixing reaction in the main chamber 100.

[0053] See also Figure 7 In one embodiment, the quantitative mixing structure 11 further includes a sample inlet channel 500 connected to the sample inlet 101, that is, the sample inlet channel 500 is connected between the sample inlet channel 12 and the sample inlet 101. The microfluidic chip 10 includes a plurality of quantitative mixing structures 11, and each sample inlet channel 500 is connected to the sample inlet channel 12 in parallel. A switch valve 600 is provided in each sample inlet channel 500, and the switch valve 600 is used to open or cut off the sample inlet channel 500. In this way, by selecting the corresponding switch valve 600 to open, the sample can be controlled to enter the corresponding quantitative mixing structure 11 for reaction.

[0054] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0055] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, and these modifications and improvements fall within the scope of protection of the present application. Therefore, the scope of protection of the present patent application shall be determined by the appended claims.

Claims

1. A quantitative mixing structure, characterized in that: The quantitative mixing structure: a main chamber, the main chamber being provided with a sample inlet for entry, the main chamber being configured to allow the liquid level of the sample to rise and fill along a reference direction, the main chamber comprising a bottom and a peripheral portion connected to a periphery of the bottom, the peripheral portion comprising a neck; a breathable valve, the breathable valve being in communication with the main chamber, and being spaced apart from the injection port and the bottom of the main chamber along the reference direction; the breathable valve allowing gas to flow out of the main chamber and preventing liquid from flowing out of the main chamber; In which, the neck is provided with a communication port connected to the air valve, and along the reference direction, the bottom, the communication port and the injection port are arranged in sequence, and the neck divides the space inside the main chamber into a mixing chamber and a quantitative chamber. The mixing chamber and the quantitative chamber are respectively located on both sides of the neck and are connected to each other. The injection port is opened on the side where the mixing chamber is located, and the sample reagent to be reacted is preset in the mixing chamber.

2. The quantitative mixing structure according to claim 1, characterized in that: The bottom and the circumferential portion together form a cavity of the main chamber, and are used for the liquid level of the sample to rise from the bottom along the circumferential portion to fill the cavity.

3. The quantitative mixing structure according to claim 2, characterized in that: The neck is retracted into the main chamber relative to other areas of the circumferential portion, and the shortest distances from various areas of the plane where the neck is located to the plane where the bottom is located are equal.

4. The quantitative mixing structure according to claim 3, characterized in that: The cross-sectional size of the mixing cavity gradually decreases in a direction approaching the neck.

5. The quantitative mixing structure according to claim 4, characterized in that: The quantitative cavity comprises a first closing area relatively close to the neck, and the cross-sectional size of the first closing area gradually decreases in a direction close to the neck.

6. The quantitative mixing structure according to claim 4, characterized in that: It also includes a stop valve and a waste liquid chamber. The stop valve is arranged at the bottom and communicates between the main chamber and the waste liquid chamber. The stop valve is used to connect or cut off the main chamber and the waste liquid chamber.

7. The quantitative mixing structure according to claim 6, characterized in that: The quantitative chamber includes a second closing area relatively close to the waste liquid chamber, and the cross-sectional size of the second closing area gradually decreases along the direction close to the waste liquid chamber.

8. A microfluidic chip, characterized in that: The microfluidic chip comprises the quantitative mixing structure according to any one of claims 1 to 7.

9. The microfluidic chip according to claim 8, characterized in that: It also includes an injection flow channel, which is connected to the injection port and is used for allowing the sample to flow into the main chamber; a one-way valve is provided in the injection flow channel, and the one-way valve is used for allowing the sample to flow in one direction to the injection port.

10. The microfluidic chip according to claim 9, characterized in that: The quantitative mixing structure also includes a sample inlet channel connected to the sample inlet. The microfluidic chip includes multiple quantitative mixing structures, and each of the sample inlet channels is connected to the sample inlet channel in parallel; a switch valve is provided in each of the sample inlet channels, and the switch valve is used to open or cut off the sample inlet channel.

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