A porcine reproductive and respiratory syndrome virus fluorescence PCR detection kit and its application

Abstract

The present invention relates to the technical field of test kits, specifically to a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit and its application, comprising two fixedly connected basic box bodies, the internal and external structures of the two basic box bodies being identical, the basic box bodies as a whole being a rectangular block without a top surface and hollow inside, characterized in that a top cover is rotatably connected between the inner walls on both sides of the basic box body, a lifting mechanism and a shaking mechanism are provided inside the basic box body, the lifting mechanism is located below the shaking mechanism, and the lifting mechanism includes a pull-out plate and a connected plate. By providing a second pull-out sleeve, pulling the second pull-out sleeve can simultaneously start the operation of the lifting mechanism and the shaking mechanism, the lifting mechanism can increase the vertical height of each reagent tube for easy direct access, and during this process, the reagent inside the reagent tube will be fully shaken by the shaking mechanism, which is conducive to efficient operation.

Description

Technical Field

[0001] The invention belongs to the technical field of kits, and in particular relates to a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit and an application thereof. Background Art

[0002] The Porcine Reproductive and Respiratory Syndrome Virus Fluorescent PCR Detection Kit is an efficient and accurate diagnostic tool specifically designed for the rapid detection of PRRSV viral RNA in pig serum, lungs, tonsils and other samples, helping the pig industry to promptly detect and control epidemics and ensure the health of pig herds.

[0003] For example, the Chinese patent publication number CN206751854U proposes a genetic testing kit with a drawer where necessary items can be placed. If reagents that require a low temperature need to be stored, ice packs can be placed in the drawer to effectively lower the temperature and protect the data values ​​of the reagents. Physical examination forms, information, etc. can also be placed in the drawer. An insulating layer is provided to maintain the temperature required by the worker for a long time, making it convenient for the storage of reagents. A portable bag is provided to facilitate the staff to take out the reagent placement plate.

[0004] However, when the upper end of the test kit is opened, the reagents are still located in a relatively deep position in the test kit, which is still inconvenient to remove. Moreover, the reagents still need to be mixed manually after removal, which reduces efficiency. Therefore, we propose a porcine reproductive and respiratory syndrome virus fluorescence PCR detection kit and its application. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit and its application to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: A porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit, comprising two fixedly connected basic box bodies, the internal and external structures of the two basic box bodies are exactly the same, the basic box body as a whole is a rectangular block without a top surface and hollow inside, a top cover is rotatably connected between the inner walls on both sides of the basic box body, a lifting mechanism and a shaking mechanism are arranged inside the basic box body, the lifting mechanism is located below the shaking mechanism, the lifting mechanism includes a pull-out plate and a connecting plate, the connecting plate is arranged above the pull-out plate, a receiving plate is slidably connected between the inner walls on both sides of the basic box body, the bottom end of the receiving plate is fixedly connected to the bottom box, the shaking mechanism includes a linkage component and four cylinders 1, the linkage component includes two drive bars and a gear column, the drive bar is in a meshing relationship with the gear column, and a cylinder 2 is provided on the inner wall of each cylinder 1.

[0007] Preferably, the linkage assembly is arranged inside the bottom box, the interior of the receiving plate passes through and is rotatably connected with four connecting shafts, the top of each connecting shaft is fixedly connected to the bottom end of each cylinder one, the bottom end of each connecting shaft is fixedly connected to a gear one, the outer wall of each gear one is meshed with the same synchronous belt, the bottom ends of the two connecting shafts with the largest spacing are fixedly connected to a gear column, the outer walls of the two gear columns are meshed with a driving strip, one end of the two driving strips is fixedly connected to the same drawing sleeve one, one side outer wall of the basic box body is provided with a side groove, the drawing sleeve one passes through the side groove, and the drawing sleeve one is fixedly connected to two auxiliary parts positioned far away from each other on the outer wall of the side close to the basic box body, one end of the inner wall of the basic box body is fixedly connected to four sliding rods, and the interior of the two auxiliary parts and the driving strips are in a sliding connection with the outer wall of each sliding rod.

[0008] Preferably, the overall weight of the cylinder 2 is larger and larger than the cylinder 1, and a plurality of equidistantly arranged annular grooves are opened on the outer wall of the cylinder 2, and a plurality of shaft-mounted balls are rotatably connected between the two ends of the inner wall of the annular groove, and the plurality of shaft-mounted balls are in a rolling connection relationship with the inner wall of the cylinder 1. The cylinder 2 as a whole is a cylinder without a top surface and hollow inside, and the inner wall of the cylinder 2 is rough. A reagent tube is clamped inside the cylinder 2, and a protrusion 1 is fixedly connected to the top of the cylinder 1, and the protrusion 1 is slidably connected to the outer wall of the cylinder 2. The outer wall of the cylinder 2 is fixedly connected to the protrusion 2, and an arc spring 1 is fixedly connected between the protrusion 1 and the protrusion 2. The arc spring 1 is internally slidably connected and is sleeved with an arc rod, one end of the arc rod passes through one side of the protrusion 1 and is fixedly connected with the protrusion 3, and the protrusion 3 is fixedly connected with the protrusion 1. The arc spring 2 is sleeved on the outer wall of the arc rod and is in a sliding connection with it.

[0009] Preferably, a closing cover is provided on the top of the reagent tube, and a curved stirring blade is fixedly connected to the top end of the inner wall of the closing cover.

[0010] Preferably, the bottom end of the top cover is fixedly connected to a large sponge block, and the bottom end of the large sponge block is provided with four circular grooves, and the two circular grooves located in the middle are combined into one, and the bottom end of the inner wall of each circular groove is fixedly connected to a rough pad, and one end of the top cover is provided with two square grooves that are far away from each other and symmetrically positioned, and the inside of the square groove is slidably connected with a card strip, one end of the card strip is fixedly connected to a connecting block, and one end of the connecting block is fixedly connected to a plurality of equidistantly arranged rubber strips, and the inside of the basic box body is provided with two limiting grooves that are far away from each other and symmetrically positioned, and the card strip can be clipped into the inside of the limiting groove.

[0011] Preferably, the inner cavity of the side groove is slidably connected with a side block, the bottom end of the side block is movably connected to the top of the pull-out sleeve 2, both ends of the inner wall of the basic box body are fixedly connected with a vertical block 1, the bottom ends of the two vertical blocks 1 are fixedly connected to the bottom end of the inner wall of the basic box body, the interiors of the two vertical blocks 1 are slidably connected with a vertical block 2, and the top of the vertical block 2 can pass through the top of the vertical block 1.

[0012] Preferably, a long through groove is provided on one side outer wall of the basic box body, and the long through groove is in a through shape as a whole, and a connecting rope is slidably connected to the inside of the long through groove, and a pulling sleeve 2 is fixedly connected between the connecting rope and the pulling sleeve 1, and an auxiliary cross plate is slidably connected to the inside of the pulling plate, and one end of the auxiliary cross plate passes through one end of the pulling plate and is fixedly connected to one end of the inner wall of the basic box body, and a connecting assembly is provided on the top of the pulling plate, and the connecting assembly includes an upper connecting member and a lower connecting member, and the upper connecting member and the lower connecting member both include two support seats and a cross bar fixedly connected between the two support seats, and the connecting plate and the two cross bars are in a rotational connection relationship.

[0013] Preferably, auxiliary blocks are fixedly connected to the outer walls of both sides of the pull-out plate, a combination groove is provided on one side of the vertical block close to the pull-out plate, and a plurality of rolling balls are rollingly connected between the top and bottom ends of the auxiliary blocks and the inner walls of the combination groove.

[0014] Preferably, vertical grooves are provided on both side outer walls of the basic box body, a slide bar is slidably connected inside the vertical groove, a card block is fixedly connected to one end of the slide bar, a metal block is fixedly connected inside the card block, and the cross-sections of the vertical grooves, slide bar and card block are all bent and the angle is 90°.

[0015] The application steps of a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit are as follows:

[0016] Step 1: Pull up each card block so that its bottom is flush with the base box body, then separate the two reagent kits stacked together and place them on the work table.

[0017] Step 2: Pull up each connecting block so that the card bar returns to the inside of the square groove, losing its limiting effect on the top cover, and then flip the top cover upward along the rotating connection between the top cover and the basic box body to expose a part of the interior of the basic box body.

[0018] Step 3: Pull the connecting rope, which drives the pull-out plate and the pull-out sleeve 1 at the same time, that is, starts the lifting mechanism and the shaking mechanism at the same time, so that each closing cover completes the shaking of the reagent while moving to the convenient position.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] By setting up the pull-out sleeve 2, pulling the pull-out sleeve 2 can simultaneously start the operation of the lifting mechanism and the shaking mechanism. The lifting mechanism can increase the vertical height of each reagent tube for direct removal. During this process, the reagent inside the reagent tube will be fully shaken by the action of the shaking mechanism, which is conducive to efficient operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0022] Figure 2 For the present invention Figure 1 A magnified schematic diagram of the structure at point A.

[0023] Figure 3 This is a schematic diagram of the connection between the top cover and the large sponge block of the present invention.

[0024] Figure 4 It is a schematic diagram of the connection relationship between the receiving plate and other components of the present invention.

[0025] Figure 5 For the present invention Figure 4 A magnified schematic diagram of the structure at point B.

[0026] Figure 6 Schematic diagram of the connection relationship between the lifting mechanism and cylinder 1 of the present invention.

[0027] Figure 7 It is a schematic diagram of the overall structure of a half-section of the cylinder of the present invention.

[0028] Figure 8 It is a schematic diagram of the overall cross-sectional structure of the present invention.

[0029] Figure 9 For the present invention Figure 8 Enlarged schematic diagram of the structure at C.

[0030] Figure 10 This is a schematic diagram of the overall cross-sectional structure of the present invention from another perspective.

[0031] Figure 11 For the present invention Figure 8 Enlarged schematic diagram of the structure at D.

[0032] In the figure: 1. Basic box; 2. Top cover; 3. Square groove; 4. Card strip; 5. Connecting block; 6. Rubber strip; 7. Large sponge; 8. Circular groove; 9. Rough pad; 10. Adapter plate; 11. Connecting shaft; 12. Gear 1; 13. Synchronous belt; 14. Gear column; 15. Drive bar; 16. Slider; 17. Auxiliary part; 18. Cylinder 1; 19. Cylinder 2; 20. Bump 1; 21. Bump 2; 22. Arc rod; 23. Arc spring 1; 24. Arc spring 2; 25. Bump 3 26. Annular groove; 27. Ball bearing with shaft; 28. Reagent tube; 29. ​​Closing cover; 30. Curved stirring blade; 31. Vertical block 1; 32. Vertical block 2; 33. Bottom box; 34. Pull-out plate; 35. Auxiliary horizontal plate; 36. Connecting assembly; 37. Connecting plate; 38. Pull-out sleeve 1; 39. Pull-out sleeve 2; 40. Connecting rope; 41. Limiting groove; 42. Side block; 43. Side groove; 44. Long through groove; 45. Combination groove; 46. Vertical groove; 47. Slide bar; 48. Block; 49. Metal block. DETAILED DESCRIPTION

[0033] In order to clearly and completely describe the objectives and technical solutions of the present invention and make its advantages more clearly understood, the embodiments of the present invention are further described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only part of the embodiments of the present invention, not all of them, and are only used to explain the embodiments of the present invention, not to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] For example 1, please refer to Figures 1 to 11The present invention provides a technical solution: a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit, comprising two fixedly connected basic box bodies 1, the internal and external structures of the two basic box bodies 1 are exactly the same, the basic box body 1 as a whole is a rectangular block without a top surface and hollow inside, a top cover 2 is rotatably connected between the inner walls on both sides of the basic box body 1, a lifting mechanism and a shaking mechanism are arranged inside the basic box body 1, the lifting mechanism is located below the shaking mechanism, the lifting mechanism includes a pull-out plate 34 and a connecting plate 37, the connecting plate 37 is arranged above the pull-out plate 34, a receiving plate 10 is slidably connected between the inner walls on both sides of the basic box body 1, the bottom end of the receiving plate 10 is fixedly connected to the bottom box 33, the shaking mechanism includes a linkage component and four cylinders 18, the linkage component includes two driving bars 15 and a gear column 14, the driving bar 15 is in a meshing relationship with the gear column 14, and a cylinder 2 19 is provided on the inner wall of each cylinder 18. By setting the pull-out sleeve 2 39, pulling the pull-out sleeve 2 39 can simultaneously start the operation of the lifting mechanism and the shaking mechanism. The lifting mechanism can increase the vertical height of each reagent tube 28 to facilitate direct taking. During this process, the reagent inside the reagent tube 28 will be fully shaken by the action of the shaking mechanism, which is conducive to the efficient operation.

[0035] For example 2, please refer to Figures 1 to 11On the basis of the first embodiment, the linkage assembly is arranged inside the bottom box 33, and the interior of the receiving plate 10 passes through and is rotatably connected with four connecting shafts 11. The top of each connecting shaft 11 is fixedly connected to the bottom end of each cylinder 18, and the bottom end of each connecting shaft 11 is fixedly connected to a gear 12. The outer wall of each gear 12 is engaged with the same synchronous belt 13. The bottom ends of the two connecting shafts 11 with the largest spacing are fixedly connected to a gear column 14. The outer walls of the two gear columns 14 are engaged with a drive bar 15. The two drive bars 15 are One end is fixedly connected with the same drawing sleeve 38, and a side groove 43 is provided on the outer wall of one side of the basic box body 1. The drawing sleeve 38 passes through the side groove 43. The outer wall of the drawing sleeve 38 close to the basic box body 1 is fixedly connected with two auxiliary parts 17 at positions far away from each other. One end of the inner wall of the basic box body 1 is fixedly connected with four slide bars 16. The interior of the two auxiliary parts 17 and the driving bar 15 are all in a sliding connection with the outer wall of each slide bar 16. The overall weight of the cylinder 2 19 is larger and larger than the cylinder 18. The outer wall of the cylinder 2 19 is provided with a groove 43. There are multiple annular grooves 26 arranged at equal intervals, and a number of shaft balls 27 are rotatably connected between the two ends of the inner wall of the annular groove 26. The several shaft balls 27 are all in rolling connection with the inner wall of the cylinder 18. The cylinder 2 19 is a cylinder without a top surface and hollow inside. The inner wall of the cylinder 2 19 is rough. A reagent tube 28 is clamped inside the cylinder 2 19. The top of the cylinder 18 is fixedly connected to a protrusion 20, and the protrusion 20 is slidably connected to the outer wall of the cylinder 2 19. The outer wall of the cylinder 2 19 is fixedly connected to a protrusion 21. An arc spring 1 23 is fixedly connected between block 1 20 and protrusion 2 21. The interior of the arc spring 1 23 is slidably connected and is sleeved with an arc rod 22. One end of the arc rod 22 passes through one side of protrusion 1 20 and is fixedly connected with protrusion 3 25. An arc spring 24 is fixedly connected between protrusion 3 25 and protrusion 1 20. The arc spring 24 is sleeved on the outer wall of the arc rod 22 and is in a sliding connection with it. A closing cover 29 is provided on the top of the reagent tube 28, and a curved stirring blade 30 is fixedly connected to the top of the inner wall of the closing cover 29.

[0036] By pulling the two pulling sleeves 138 quickly and simultaneously, the displacement of the driving bar 15 drives the tooth column 14 to rotate under the connection of the two driving bars 15 and the tooth column 14, thereby rotating the coaxial gear 12. Since the four gears 12 are in meshing relationship with the timing belt 13, each gear 12 will rotate together, and each fixedly connected connecting shaft 11 will also rotate synchronously. The cylinder 18 fixedly connected at one end of the connecting shaft 11 will also rotate synchronously. Since the overall weight of the cylinder 2 19 is larger and larger than the cylinder 18, and since a number of rolling-connected shaft balls 27 are provided between the cylinder 18 and the cylinder 2 19, there is a time difference in the movement of the cylinder 18 and the cylinder 2 19, the priority of the rotation of the cylinder 18 is higher than that of the cylinder 2 19, and the cylinder 18 rotates first, and its top is fixed. The connected protrusion 1 20 rotates synchronously in the same direction, approaching the protrusion 3 25 and compressing the arc spring 2 24, causing the arc spring 1 23 to stretch. When the arc spring 1 23 is compressed to its limit, the arc spring 2 24 and the arc spring 1 23 gradually recover their deformation. During this process, the cylinder 2 19 rotates in the opposite direction of the cylinder 1 18. The arc springs 1 23 and 24 cause the cylinder 2 19 to rotate alternately clockwise and counterclockwise, and this reciprocating motion shakes and impacts the reagent inside the reagent tube 28 engaged therewith. The curved stirring blades 30 act as a flow turbulent, allowing the reagent inside the reagent tube 28 to be evenly shaken during this movement, facilitating direct use after unscrewing the closure cap 29, thereby improving efficiency. The rough inner wall of the cylinder 2 19 increases friction between the outer wall of the reagent tube 28 and the cylinder 2 19, improving stability and facilitating the real-time transmission of centripetal force. The annular grooves 26 and the shaft-mounted balls 27 also reduce the friction between the outer wall of the cylinder 2 19 and the inner wall of the cylinder 1 18 when the cylinder 2 19 rotates, thereby improving the smoothness of the rotation of the cylinder 2 19 and the closing cover 29, thereby facilitating the improvement of the mixing effect.

[0037] For example three, please refer to Figures 1 to 11On the basis of embodiment 2, the bottom end of the top cover 2 is fixedly connected with a large sponge block 7, and the bottom end of the large sponge block 7 is provided with four circular grooves 8. The two circular grooves 8 in the middle position are combined into one body. The bottom end of the inner wall of each circular groove 8 is fixedly connected with a rough pad 9. One end of the top cover 2 is provided with two square grooves 3 that are far away from each other and symmetrical in position. The inside of the square groove 3 is slidably connected with a card strip 4, one end of the card strip 4 is fixedly connected to a connecting block 5, and one end of the connecting block 5 is fixedly connected to a plurality of rubber strips 6 arranged at equal distances. The interior of the basic box body 1 is provided with two square grooves 3 that are far away from each other and symmetrical in position. The limiting grooves 41 are symmetrically arranged, and the card strip 4 can be snapped into the inside of the limiting groove 41. The inner cavity of the side groove 43 is slidably connected with the side block 42. The bottom end of the side block 42 is movably connected to the top of the pull-out sleeve 2 39. The two ends of the inner wall of the basic box body 1 are fixedly connected with the vertical block 1 31. The bottom ends of the two vertical blocks 1 31 are fixedly connected to the bottom end of the inner wall of the basic box body 1. The interiors of the two vertical blocks 1 31 are slidably connected with the vertical block 2 32. The top of the vertical block 2 32 can pass through the top of the vertical block 1 31. A long through groove 44 is provided on the outer wall of one side of the basic box body 1. The long through groove 44 is integrally formed. The inner sliding connection of the long through groove 44 is provided with a connecting rope 40, and the connecting rope 40 is fixedly connected to the pull sleeve 1 38 with a pull sleeve 2 39. The inner sliding connection of the pull plate 34 is provided with an auxiliary cross plate 35, and one end of the auxiliary cross plate 35 passes through one end of the pull plate 34 and is fixedly connected to one end of the inner wall of the basic box body 1. The top of the pull plate 34 is provided with a connecting assembly 36, and the connecting assembly 36 includes an upper connecting member and a lower connecting member. The upper connecting member and the lower connecting member each include two support seats and a cross bar fixedly connected between the two support seats. The connecting plate 37 and the two cross bars are formed The rotational connection relationship is that the outer walls of both sides of the pull-out plate 34 are fixedly connected with auxiliary blocks, and a combination groove 45 is provided on the side of the vertical block 31 close to the pull-out plate 34. A plurality of rolling balls are rollingly connected between the top and bottom ends of the auxiliary block and the inner wall of the combination groove 45. The outer walls of both sides of the basic box body 1 are provided with vertical grooves 46, and the interior of the vertical groove 46 is slidably connected with a slide bar 47, one end of the slide bar 47 is fixedly connected with a card block 48, and the interior of the card block 48 is fixedly connected with a metal block 49. The cross sections of the vertical groove 46, the slide bar 47 and the card block 48 are all bent and the angle is 90°.

[0038] By setting the large sponge block 7, in the initial state, that is, when the top cover 2 has not been opened, each closing cover 29 is respectively located inside each circular groove 8 and contacts each rough pad 9. The large sponge block 7 is in a slightly compressed state as a whole, and at the same time, it has a certain pressure on the top of each closing cover 29, so that each closing cover 29 can be in a stable state when not taken out, which is conducive to storage or transportation. The two card strips 4 are also respectively engaged in the two limit grooves 41. At this time, the top cover 2 cannot be directly flipped open. Such a setting can effectively prevent the accidental opening of the test kit as a whole, and the rubber strip 6 can be pushed up to drive The card strip 4 is returned to the inside of the square groove 3, losing its fixing effect, which is convenient for the subsequent removal of the closed cover 29. The setting of the rubber strip 6 can increase the friction between the fingers or gloves, which is convenient for adjusting the position of the card strip 4. When the pull-out sleeve 39 is pulled, the pull-out plate 34, the connecting plate 37 and the connecting assembly 36 are set. Since the connecting assembly 36 includes an upper connecting member and a lower connecting member, the upper connecting member and the lower connecting member both include two support seats and a cross bar fixedly connected between the two support seats, and the connecting plate 37 and the two cross bars are in a rotational connection relationship, the displacement of the pull-out plate 34 will cause the lower connecting member to synchronously drive the connecting plate 37 Because the position of the receiving plate 10 is restricted by the inner walls of the basic box body 1, it will not move synchronously, but will move in the vertical direction. Then, the vertical height of each closing cover 29 can be increased. Then, as the pull-out plate 34 is pulled, the closing cover 29 will move to a position that is more convenient for removal. By pulling the connecting rope 40, the connecting rope 40 can be pulled to simultaneously drive the pull-out plate 34 and the pull-out sleeve 38, that is, the lifting mechanism and the shaking mechanism are started at the same time, so that each closing cover 29 completes the shaking of the reagent while moving to the convenient position, killing two birds with one stone, which is conducive to improving work efficiency. The vertical block 32, The setting of the auxiliary block and the rolling ball respectively improves the stability of the receiving plate 10 during displacement and the smoothness and stability of the pull-out plate 34 during displacement. When the two test kits are stacked together as a whole, the upper block 48 can be slid downward. At this time, the slide bar 47 is displaced from the top of the inner wall of the vertical groove 46 to the lower end. Since the block 48 is bent and the angle is 90°, it will be clamped to the top of the test kit below, that is, the corner of the top of the two basic box bodies 1, thereby improving the stability when stacked together. The metal block 49 improves the stability in this state and improves the stability of the test kit as a whole when placed on the desktop.

[0039] The application steps of a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit are as follows:

[0040] Step 1: Pull up each card block 48 so that its bottom end is flush with the basic box body 1, then separate the two reagent boxes stacked together and place them on the work table.

[0041] Step 2: Pull up each connecting block 5 so that the card strip 4 returns to the inside of the square groove 3, losing its limiting effect on the top cover 2, and then flip the top cover 2 upward along the rotation connection between the top cover 2 and the basic box body 1 to expose a part of the interior of the basic box body 1.

[0042] Step 3: Pull the connecting rope 40, which drives the pull-out plate 34 and the pull-out sleeve 38 at the same time, that is, starts the lifting mechanism and the shaking mechanism at the same time, so that each closing cover 29 completes the shaking of the reagent while moving to the convenient position.

[0043] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A porcine reproductive and respiratory syndrome virus fluorescence PCR detection kit, comprising two fixedly connected basic boxes (1), wherein the internal and external structures of the two basic boxes (1) are identical, and the basic boxes (1) are generally rectangular blocks without a top surface and having a hollow interior, characterized in that: A top cover (2) is rotatably connected between the inner walls on both sides of the basic box body (1), and a lifting mechanism and a shaking mechanism are provided inside the basic box body (1), wherein the lifting mechanism is located below the shaking mechanism, and the lifting mechanism comprises a pull-out plate (34) and a connecting plate (37), wherein the connecting plate (37) is provided above the pull-out plate (34), and a receiving plate (10) is slidably connected between the inner walls on both sides of the basic box body (1), and the bottom end of the receiving plate (10) is fixedly connected to the bottom box (33), and the shaking mechanism comprises a linkage component and four cylinders (18), wherein the linkage component comprises two drive bars (15) and a tooth column (14), wherein the drive bar (15) is in meshing relationship with the tooth column (14), and a cylinder (19) is provided on the inner wall of each cylinder (18);Four connecting shafts (11) are passed through and rotatably connected to the interior of the receiving plate (10), the top of each connecting shaft (11) is fixedly connected to the bottom of each cylinder (18), the bottom of each connecting shaft (11) is fixedly connected to a gear (12), the outer wall of each gear (12) is engaged with the same synchronous belt (13), the bottom ends of the two connecting shafts (11) with the largest spacing are fixedly connected to a tooth column (14), the outer walls of the two tooth columns (14) are engaged with a driving bar (15), and one end of the two driving bars (15) is fixedly connected to the same pull-out sleeve (3 8), a plurality of equally spaced annular grooves (26) are provided on the outer wall of the second cylinder (19), a plurality of shaft-bearing balls (27) are rotatably connected between the two ends of the inner wall of the annular groove (26), a convex block (20) is fixedly connected to the top of the first cylinder (18), the convex block (20) is slidably connected to the outer wall of the second cylinder (19), the outer wall of the second cylinder (19) is fixedly connected to the convex block (21), an arc spring (23) is fixedly connected between the convex block (20) and the convex block (21), the interior of the arc spring (23) is slidably connected and sleeved with an arc rod (22), One end of the arc rod (22) passes through one side of the protrusion one (20) and is fixedly connected to the protrusion three (25), and an arc spring two (24) is fixedly connected between the protrusion three (25) and the protrusion one (20). One end of the top cover (2) is provided with two square grooves (3) that are away from each other and symmetrical in position. The interior of the square groove (3) is slidably connected to a clamping strip (4), and one end of the clamping strip (4) is fixedly connected to a connecting block (5). A long through groove (44) is provided on one side of the outer wall of the basic box body (1), and a connecting rope (40) is slidably connected to the interior of the long through groove (44). The connecting rope (40) is slidably connected to the interior of the long through groove (44). 0) is fixedly connected to the drawer sleeve 1 (38) with a drawer sleeve 2 (39), the top of the drawer plate (34) is provided with a connecting assembly (36), the connecting assembly (36) includes an upper connecting member and a lower connecting member, the upper connecting member and the lower connecting member each include two support seats and a cross bar fixedly connected between the two support seats, the connecting plate (37) and the two cross bars are in a rotational connection relationship, the outer walls of both sides of the basic box body (1) are provided with vertical grooves (46), the interior of the vertical grooves (46) is slidably connected to a slide bar (47), and one end of the slide bar (47) is fixedly connected to a block (48). ; 2. A porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 1, characterized in that: The linkage assembly is arranged inside the bottom box (33), and a side groove (43) is opened on one side outer wall of the basic box body (1). The pull-out sleeve (38) passes through the side groove (43). The pull-out sleeve (38) is fixedly connected to the outer wall of one side close to the basic box body (1) with two auxiliary parts (17) at positions far away from each other. One end of the inner wall of the basic box body (1) is fixedly connected to four sliding rods (16), and the interiors of the two auxiliary parts (17) and the driving bar (15) are in a sliding connection relationship with the outer walls of each sliding rod (16).

3. A porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 1, characterized in that: The overall weight of the cylinder 2 (19) is relatively large and greater than that of the cylinder 1 (18). Several of the shaft-bearing balls (27) are in rolling connection with the inner wall of the cylinder 1 (18). The cylinder 2 (19) is a cylinder without a top surface and hollow inside. The inner wall of the cylinder 2 (19) is rough. A reagent tube (28) is clamped inside the cylinder 2 (19). The arc spring 2 (24) is sleeved on the outer wall of the arc rod (22) and is in sliding connection with it.

4. A porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 3, characterized in that: A closing cover (29) is provided on the top of the reagent tube (28), and a curved stirring blade (30) is fixedly connected to the top of the inner wall of the closing cover (29).

5. The porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 1, characterized in that: The bottom end of the top cover (2) is fixedly connected to a large sponge block (7), and the bottom end of the large sponge block (7) is provided with four circular grooves (8). The two circular grooves (8) located in the middle are combined into one body, and the bottom end of the inner wall of each circular groove (8) is fixedly connected to a rough pad (9). One end of the connecting block (5) is fixedly connected to a plurality of equidistantly arranged rubber strips (6). The interior of the basic box body (1) is provided with two mutually distant and symmetrical limiting grooves (41), and the clamping strip (4) can be clamped inside the limiting groove (41).

6. A porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 2, characterized in that: The inner cavity of the side groove (43) is slidably connected to the side block (42), and the bottom end of the side block (42) is movably connected to the top of the drawer sleeve 2 (39). Both ends of the inner wall of the basic box body (1) are fixedly connected to the vertical block 1 (31), and the bottom ends of the two vertical blocks 1 (31) are fixedly connected to the bottom end of the inner wall of the basic box body (1). The interiors of the two vertical blocks 1 (31) are slidably connected to the vertical block 2 (32), and the top of the vertical block 2 (32) can pass through the top of the vertical block 1 (31).

7. The porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 1, characterized in that: The long through slot (44) is in a through shape as a whole, and an auxiliary transverse plate (35) is slidably connected to the interior of the pull-out plate (34), and one end of the auxiliary transverse plate (35) passes through one end of the pull-out plate (34) and is fixedly connected to one end of the inner wall of the basic box body (1).

8. The porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 6, characterized in that: Auxiliary blocks are fixedly connected to the outer walls of both sides of the pull-out plate (34), and a combination groove (45) is provided on the side of the vertical block (31) close to the pull-out plate (34). A plurality of rolling balls are rollingly connected between the top and bottom ends of the auxiliary blocks and the inner walls of the combination groove (45).

9. The porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to claim 1, characterized in that: A metal block (49) is fixedly connected to the interior of the clamping block (48), and the cross sections of the vertical slot (46), the slide bar (47) and the clamping block (48) are all bent and have an angle of 90°.

10. An application of a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit, characterized in that: The method comprises a porcine reproductive and respiratory syndrome virus fluorescent PCR detection kit according to any one of claims 1 to 9, wherein the method comprises the following steps: Step 1: Pull up each card block (48) so that its bottom end is flush with the basic box body (1), and then separate the two reagent boxes stacked together and place them on the work table; Step 2: Pull up each connecting block (5) so that the clamping strip (4) returns to the inside of the square groove (3), thereby losing the limiting effect on the top cover (2), and then flip the top cover (2) upward along the rotation connection between the top cover (2) and the basic box body (1), so that a part of the interior of the basic box body (1) is exposed; Step 3: Pull the connecting rope (40), which simultaneously drives the pull-out plate (34) and the pull-out sleeve (38), that is, simultaneously starts the lifting mechanism and the shaking mechanism, so that each closing cover (29) completes the shaking of the reagent while moving to the convenient position.

Images