A pretreatment device for culturing human umbilical cord mesenchymal stem cells

Abstract

The invention relates to the technical field of cell culture pretreatment, and specifically discloses a pretreatment device for culturing human umbilical cord mesenchymal stem cells, comprising a box body, a centrifugal stirring mechanism is fixedly installed at the lower end of the box body, a stirring chamber is opened inside the box body, the bottom of the centrifugal stirring mechanism extends inside the stirring chamber, a digestion component is placed on the top of the box body, a mounting seat is fixedly installed in the middle of the back of the box body, a telescopic cylinder cover is started, and then a driving motor is started, the motor rotates an eccentric shaft, drives a linkage frame to telescope back and forth, and pushes a top plate to move up and down, because a guide plate in a guide rail is connected to the top plate, guided by the guide plate and the guide rail, the eccentric activity force of the eccentric shaft acts on a rectangular cutting blade, so that it reciprocates in the mesh of a cutting net, and the umbilical cord segment can be continuously cut and processed, so as to avoid incomplete cutting, improve the overall cutting stability, ensure a good and continuous cutting effect, and effectively process the umbilical cord segment.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture pretreatment, in particular to a pretreatment device for culturing human umbilical cord mesenchymal stem cells. Background Art

[0002] Cultivating human umbilical cord mesenchymal stem cells has extremely broad prospects and many important uses. It has great potential in the field of regenerative medicine and can be used to treat a variety of diseases such as nervous system diseases, cardiovascular diseases, bone and cartilage injuries, etc. It can repair damaged tissues and organs by differentiating into specific cell types; in diseases related to the immune system, it can regulate immune responses and exert immune regulation functions, opening up new ways to treat autoimmune diseases; in addition, it can also be used in drug research and development and screening, providing strong support for the development of more efficient and safer drugs. At the same time, in the field of tissue engineering, it can be used as seed cells to construct various functional tissues, providing new solutions for organ transplantation, etc.

[0003] The Chinese patent with publication number "CN107475111B" discloses a human umbilical cord mesenchymal stem cell pretreatment device, which includes a flip-up cover and a cutting assembly with a grid-shaped blade and an elastic sealing ring, etc. It also provides related cell pretreatment and culture methods, including six steps such as umbilical cord chopping and separation. This method is stable and reproducible, can improve cell activity and yield, and is simple and flexible to operate;

[0004] However, during the specific operation of the above-mentioned device, the upper cover needs to be placed on the top of the device and pressed before cutting. During the specific cutting, it is usually only possible to perform a one-time cut when the cover is closed. As the human umbilical cord is a relatively soft part of the human tissue structure, it is obviously difficult to completely cut all tissues in one cut, and there is a situation of incomplete cutting. At the same time, when collecting human umbilical cord mesenchymal stem cells after centrifugal purification, it is necessary to collect them through a straw. However, due to the setting of components such as the centrifugal structure and digestion components inside the device, it is obviously extremely inconvenient to use a straw to absorb human umbilical cord mesenchymal stem cells. It can be seen that there are certain defects and deficiencies in the internal stem cell recovery and clean cutting operations as a whole, which need to be improved. Summary of the invention

[0005] The object of the present invention is to provide a pretreatment device for culturing human umbilical cord mesenchymal stem cells to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned object, the present invention provides a pretreatment device for culturing human umbilical cord mesenchymal stem cells, comprising a box body, a centrifugal stirring mechanism is fixedly installed at the lower end of the box body, a stirring chamber is opened inside the box body, the bottom of the centrifugal stirring mechanism extends inside the stirring chamber, a digestion component is placed on the top of the box body, a mounting seat is fixedly installed in the middle of the back of the box body, a telescopic cylinder is fixedly installed on the inner side of the mounting seat, a connecting rod is fixedly installed on the top output end of the telescopic cylinder through the mounting seat, a top plate is fixedly installed on the top of the connecting rod, a cutting mechanism is fixedly installed on the front of the top plate, and the cutting mechanism covers the top of the digestion component, an external electromagnetic valve is fixedly installed in the middle of the bottom of the box body, and the input end of the external electromagnetic valve is connected to the bottom of the box body;

[0007] The centrifugal stirring mechanism comprises a support tube, a rod is inserted into the support tube, a sealing disk is fixedly installed on the top of the rod, a filter cover is fixedly installed on the bottom of the sealing disk, and a stirring assembly is movably installed on the top of the sealing disk.

[0008] Furthermore, the support tube is fixedly installed at the four corners of the box body, the filter cover is composed of a stainless steel mesh cover, a layer of cell screen is fixedly connected to the inside of the filter cover, and a layer of microporous filter membrane is also fixedly connected to the inner side of the cell screen in the filter cover, an internal solenoid valve is fixedly installed at the bottom of the filter cover, a discharge pipe is fixedly connected to the bottom of the internal solenoid valve, and the lower end of the discharge pipe extends to the input end of the external solenoid valve.

[0009] Furthermore, a solution feeding pipe is fixedly installed on the upper end of one side of the box body, a solution feeding hopper is fixedly installed on the top of the solution feeding pipe, support legs are fixedly installed at the four corners of the bottom of the box body, and a non-slip bottom plate is fixedly installed on the bottom of the support legs.

[0010] Furthermore, the upper end of the interior of the mixing chamber is rectangular, the lower end of the interior of the mixing chamber is cylindrical, and the bottom of the mixing chamber is conical.

[0011] Furthermore, the stirring assembly includes a socket, which is opened at the four corners of the top of the sealing disk. The inside of the socket is fixedly connected to a plug column, and the outer surface of the plug column is fixedly connected to a layer of sealing rubber sleeve, which is used to seal the inside of the socket. An inner bracket is fixedly installed between the inner sides of the plug column, a waterproof motor is fixedly installed in the middle of the top of the inner bracket, and the output end of the waterproof motor passes through the inner bracket and is fixedly installed with a stirring frame.

[0012] Furthermore, the stirring frame includes an inner shaft, which is fixedly installed at the bottom output end of the waterproof motor, and an L-shaped stirring rod is fixedly installed at an equal interval on the outer upper end of the inner shaft, and a straight stirring rod is fixedly installed at an equal interval on the inner side of the L-shaped stirring rod.

[0013] Furthermore, a stirring paddle is fixedly installed at the bottom of the inner shaft, the stirring paddle is arranged in a spiral auger shape, and the lower end of the stirring paddle is also arranged in a cone shape. The bottom of the stirring paddle is fitly connected to the bottom of the box body, and a lifting armrest is fixedly connected to the outside of the waterproof motor.

[0014] Furthermore, the cutting mechanism includes a top cover and a cutting box, the cutting box is placed on the top of the digestion component, and an upper end of one side of the top cover is fixedly connected to a driving motor, and an output end of the driving motor passes through the top cover and is fixedly installed with an eccentric shaft, and the side shape of the eccentric shaft is concave in shape, and a linkage frame is hinged on the outer side of the eccentric shaft, and a top plate is hinged on the outer side of the linkage frame, and rectangular cutting blades are fixedly installed at equal intervals on the bottom of the top plate, and a cutting net is fixedly installed inside the cutting box, and the mesh of the cutting net is rectangular and its size corresponds to that of the rectangular cutting blade, and guide rails are fixedly installed on both sides of the interior of the top cover at both ends, and a guide plate is slidably connected to the inner side of the guide rail, and the bottom of the guide plate is fixedly connected to the outer side of the top plate.

[0015] Furthermore, the bottoms of the cutting box and the digestion component are fixedly connected with sealing frames. The sealing frame located at the bottom of the cutting box is plugged into the inner upper end of the digestion component, and the sealing frame located on the digestion component is plugged into the inner upper end of the box body. The outer surface of the sealing frame is provided with a rubber film layer for improving the sealing performance.

[0016] Furthermore, the top cover, the cutting box and the bottom of the digestion component are all fixedly connected with square sealing gaskets, the tops of the box body, the cutting box and the digestion component are all fixedly connected with square sealing grooves, and the square sealing gaskets are inserted into the inner side of the square sealing grooves.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] First, in the present invention, when in use, the umbilical cord segment is placed on the top of the cutting net, the telescopic cylinder cover is started, and then the driving motor is started. The motor rotates the eccentric shaft, drives the linkage frame to telescope back and forth, and pushes the top plate to move up and down. Because the guide plate in the guide rail is connected to the top plate, the eccentric active force of the eccentric shaft acts on the rectangular cutting blade through the guide plate and the guide rail, so that it reciprocates in the mesh of the cutting net, and the umbilical cord segment can be continuously cut and processed, avoiding the situation of incomplete cutting, improving the overall cutting stability, ensuring a good and continuous cutting effect, and effectively processing the umbilical cord segment;

[0019] Secondly, in the present invention, the device is provided with a centrifugal stirring mechanism. When the solution after cutting and digestion enters the stirring chamber, the centrifugal solution can be added through the solution feeding hopper and the solution feeding tube, and the waterproof motor is started to drive the inner shaft in the filter cover to rotate, thereby driving the L-shaped stirring rod and the straight stirring rod to rotate to stir the inside, promoting the solution and umbilical cord stem cells to be fully mixed and centrifuged. The spiral auger-shaped stirring paddle can further roll the solution and the material to make them more evenly mixed, improve the centrifugal mixing quality, and enhance the overall use performance, thereby improving the efficiency of the pretreatment of human umbilical cord stem cells, so that the entire pretreatment process is more efficient and better quality;

[0020] Thirdly, in the present invention, the setting of the centrifugal stirring mechanism brings many conveniences. When in use, the external solenoid valve can be directly opened to discharge the solution, and the solution is discharged therethrough. At the same time, the stem cells are filtered and screened by the microporous filter membrane and cell screen of the filter cover and retained on the inside. After the waste water is discharged, the internal solenoid valve is opened, and the waterproof motor is started to rotate the spiral stirring paddle, so that the purified stem cells can be spirally squeezed and discharged to the outside through the internal solenoid valve, which is convenient for rapid processing of the umbilical cord solution and improves the convenience of taking and placing the purified umbilical cord stem cells. When cleaning, the telescopic cylinder is started to separate the top cover from the cutting box, the cutting box and the digestion component are taken out, the support tube is pulled out to pull out the centrifugal stirring mechanism, and the plug column is pulled out to separate the stirring frame and the filter cover. In this way, the stirring bin, stirring frame, filter cover, digestion component and box body are convenient for subsequent cleaning, inspection and maintenance, which greatly improves the overall convenience of use of the device and makes operation and maintenance more efficient and simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the rear view structure of the present invention;

[0023] Figure 3 It is a schematic diagram of the overall structure of the cutting mechanism in the present invention in a disassembled state;

[0024] Figure 4 This is a schematic diagram of the structure of the cutting mechanism of the present invention in a disassembled state when viewed from above;

[0025] Figure 5 It is a structural schematic diagram of the connection state of the eccentric shaft, the linkage frame and the top plate in the present invention;

[0026] Figure 6 This is a schematic diagram of the structure of the centrifugal stirring mechanism removed from the box body of the present invention;

[0027] Figure 7 It is a schematic diagram of the overall structure of the centrifugal stirring mechanism in the present invention;

[0028] Figure 8It is a schematic structural diagram of the separation state of the stirring components in the centrifugal stirring mechanism of the present invention.

[0029] In the figure: 1. Box; 2. Centrifugal stirring mechanism; 21. Support tube; 22. Insert rod; 23. Sealing plate; 24. Filter cover; 25. Stirring assembly; 251. Socket; 252. Insert column; 253. Inner bracket; 254. Waterproof motor; 255. Stirring frame; 2551. Inner shaft; 2552. L-shaped stirring rod; 2553. Straight stirring rod; 2554. Stirring paddle; 2555. Lifting armrest; 26. Internal solenoid valve; 27. Discharge pipe; 3. Stirring bin; 4. Digestion assembly ; 5. Mounting seat; 6. Telescopic cylinder; 7. Connecting rod; 8. Top plate; 9. Cutting mechanism; 91. Top cover; 92. Cutting box; 93. Driving motor; 94. Eccentric shaft; 95. Linkage frame; 96. Top plate; 97. Rectangular cutting blade; 98. Cutting net; 99. Guide rail; 910. Guide plate; 911. Sealing frame; 912. Square sealing gasket; 913. Square sealing groove; 10. External solenoid valve; 11. Solution feeding pipe; 12. Solution feeding hopper; 13. Support legs. DETAILED DESCRIPTION

[0030] See also Figure 1-Figure 8 In an embodiment of the present invention, a pretreatment device for culturing human umbilical cord mesenchymal stem cells comprises a box body 1, a centrifugal stirring mechanism 2 is fixedly installed at the lower end of the box body 1, a stirring chamber 3 is opened inside the box body 1, the bottom of the centrifugal stirring mechanism 2 extends inside the stirring chamber 3, a digestion component 4 is placed on the top of the box body 1, a mounting seat 5 is fixedly installed in the middle of the back of the box body 1, a telescopic cylinder 6 is fixedly installed on the inner side of the mounting seat 5, a connecting rod 7 is fixedly installed on the top output end of the telescopic cylinder 6 through the mounting seat 5, a top plate 8 is fixedly installed on the top of the connecting rod 7, a cutting mechanism 9 is fixedly installed on the front of the top plate 8, and the cutting mechanism 9 covers the top of the digestion component 4, an external electromagnetic valve 10 is fixedly installed in the middle of the bottom of the box body 1, and the input end of the external electromagnetic valve 10 is connected to the bottom of the box body 1, and the digestion component 4 is a digestion component 4 of a human umbilical cord mesenchymal stem cell pretreatment device patent application with a publication number of "CN107475111B" in the prior art, which belongs to the prior art means, and the known technology is therefore not repeated here;

[0031] The centrifugal stirring mechanism 2 includes a support tube 21, which is fixedly installed at the four corners of the box body 1. A plug rod 22 is inserted into the support tube 21, a sealing disk 23 is fixedly installed on the top of the plug rod 22, and a filter cover 24 is fixedly installed on the bottom of the sealing disk 23. The filter cover 24 is composed of a stainless steel mesh cover, and a layer of cell screen is fixedly connected to the inside of the filter cover 24. A layer of microporous filter membrane is also fixedly connected to the inner side of the cell screen in the filter cover 24. A stirring component 25 is movably installed on the top of the sealing disk 23, an internal solenoid valve 26 is fixedly installed at the bottom of the filter cover 24, and a discharge pipe 27 is fixedly connected to the bottom of the internal solenoid valve 26. The lower end of the discharge pipe 27 extends to the input end of the external solenoid valve 10. The device as a whole integrates multiple functions such as cutting, stirring, centrifugation and filtration. The cutting mechanism 9 is driven by the telescopic cylinder 6 to achieve efficient cutting of the umbilical cord segment; in the centrifugal stirring mechanism 2, the support tube 21 and the insertion rod 22 cooperate to enable the sealing disk 23 to operate stably, and the filter cover 24 at the bottom of the sealing disk 23 is composed of a stainless steel mesh cover and has a cell screen and a microporous filter membrane inside, which can achieve effective filtration, and the stirring component 25 can make the solution and umbilical cord stem cells fully mixed and centrifuged to improve the pretreatment efficiency; the digestion component 4 on the top of the box body 1 cooperates with other structures to further optimize the treatment effect; the internal solenoid valve 26 and the external solenoid valve 10 can conveniently control the discharge and retention of the material. The whole device is reasonably designed, easy to operate, and the components are closely matched, which can effectively improve the effect and efficiency of the pretreatment of human umbilical cord mesenchymal stem cells, and is easy to clean and maintain.

[0032] See also Figure 1-Figure 2 A solution feeding pipe 11 is fixedly installed on the upper end of one side of the box body 1, and a solution feeding hopper 12 is fixedly installed on the top of the solution feeding pipe 11. The solution feeding hopper 12 fixedly installed on the top of the solution feeding pipe 11 can provide a larger feeding inlet, which is convenient for quickly pouring the centrifugal solution and improving the feeding efficiency; its larger volume can accommodate more solutions at one time, reducing the frequent feeding operation. Secondly, the existence of the solution feeding hopper 12 makes the feeding process smoother and the solution is not easy to spill, ensuring the cleanliness of the operating environment. Moreover, its stable connection with the solution feeding pipe 11 ensures that the solution can stably and accurately flow into the box body 1, avoiding leakage or deviation during the feeding process.

[0033] See also Figure 1-Figure 2The four corners of the bottom of the box 1 are fixedly installed with support legs 13, and the bottom of the support legs 13 is fixedly installed with an anti-skid bottom plate. The support legs 13 provide a stable supporting foundation for the entire pretreatment device, ensuring that the device can remain stable during operation and will not shake or tilt easily. This is conducive to the precise function of each component and ensures the smooth progress of the pretreatment process. The anti-skid bottom plate further enhances the stability of the device when it is placed, and can effectively prevent the device from sliding even on a relatively smooth ground, reducing the risk of accidents. Such a design makes the device safer and more reliable when in use, avoiding operational errors or equipment damage caused by instability. At the same time, the support legs 13 can also keep the box 1 at a certain distance from the ground, which is conducive to air circulation and prevents the bottom of the box 1 from being damp or affected by other environmental factors. Moreover, this structure is convenient for cleaning and maintenance of the bottom of the device, and there is enough space for operation. In addition, the anti-skid bottom plate can also reduce the impact of vibration generated during the operation of the device on the surrounding environment to a certain extent, and reduce noise propagation.

[0034] See also Figure 6 The upper end of the interior of the mixing bin 3 is rectangular, the lower end of the interior of the mixing bin 3 is cylindrical, and the bottom of the mixing bin 3 is conical. The rectangular setting of the upper end of the interior of the mixing bin 3 provides a larger space, which is conducive to the initial delivery and distribution of materials, so that the materials can enter the mixing bin 3 more evenly for subsequent processing. The cylindrical design of the lower end of the interior of the mixing bin 3 ensures good symmetry and stability during the centrifugal stirring process, and can evenly distribute the centrifugal force to improve the stirring and centrifugal effects. At the same time, the cylindrical space is also convenient for the installation and operation of the stirring component 25. The conical setting of the bottom of the mixing bin 3 has a good diversion effect, which can guide the materials to concentrate and flow smoothly to the bottom outlet, which is convenient for subsequent discharge and collection, and is also conducive to operations such as sedimentation and separation.

[0035] See also Figure 8The stirring component 25 includes a socket 251, which is opened at the four corners of the top of the sealing disk 23. The inside of the socket 251 is fixedly connected to the plug post 252, and the outer surface of the plug post 252 is fixedly connected to a layer of sealing rubber sleeve, which is used to seal the inside of the socket 251. An inner bracket 253 is fixedly installed between the inner sides of the plug post 252, and a waterproof motor 254 is fixedly installed in the middle of the top of the inner bracket 253. The output end of the waterproof motor 254 passes through the inner bracket 253 and is fixedly installed with a stirring frame 255. By arranging the socket 251 and the plug post 252 matched therewith at the four corners of the top of the sealing disk 23, a stable connection between the stirring component 25 and the sealing disk 23 is achieved to ensure that there will be no The presence of the sealing rubber sleeve further enhances the sealing effect, prevents liquid from leaking from the jack 251, and ensures the normal operation of the entire device. The inner bracket 253 provides a solid support for the waterproof motor 254 and the stirring frame 255, so that they can work stably. The waterproof motor 254 can operate safely in the liquid environment of the stirring chamber 3 to avoid damage due to contact with the liquid. Its output end drives the stirring frame 255 for stirring, which can fully mix the liquid and cells and improve the effect and efficiency of the pretreatment. The design of this stirring assembly 25 is compact and reasonable, and the various components cooperate with each other to achieve an efficient, stable and safe stirring function.

[0036] See also Figure 8 The stirring frame 255 includes an inner shaft 2551, which is fixedly installed at the bottom output end of the waterproof motor 254. An L-shaped stirring rod 2552 is fixedly installed at an equal interval on the outer upper end of the inner shaft 2551, and a straight stirring rod 2553 is fixedly installed at an equal interval on the inner side of the L-shaped stirring rod 2552. The inner shaft 2551 serves as a core connecting component to ensure the effective transmission of stirring power and the stable stirring action. The setting of the L-shaped stirring rod 2552 increases the range and strength of stirring, and can more comprehensively reach various areas in the stirring bin 3, making the stirring more sufficient and uniform. The evenly spaced L-shaped stirring rods 2552 can ensure the balance of stirring and avoid the situation of insufficient local stirring. The straight stirring rod 2553 on the inside further enhances the stirring effect, and cooperates with the L-shaped stirring rod 2552 to stir materials of different shapes and positions more carefully, which helps to improve the mixing uniformity of cells and solutions and improve the quality of pretreatment.

[0037] See also Figure 8A stirring paddle 2554 is fixedly installed at the bottom of the inner shaft 2551. The stirring paddle 2554 is arranged in a spiral auger shape. The lower end of the stirring paddle 2554 is also arranged in a conical shape. The bottom of the stirring paddle 2554 is fitted and connected to the bottom of the box body 1. A lifting armrest 2555 is fixedly connected to the outside of the waterproof motor 254. The spiral auger-shaped stirring paddle 2554 can produce a strong axial and radial mixing effect, making the stirring more sufficient and efficient, and can quickly and evenly disperse and mix the materials. The conical setting of its lower end is consistent with the conical shape of the bottom of the mixing bin 3. It can better handle the materials at the bottom and ensure that the materials at the bottom can also be fully stirred, avoiding the formation of dead corners and precipitation. The bottom of the stirring paddle 2554 is tightly connected to the bottom of the box body 1, which further enhances the stirring effect and the effect on the bottom materials. The lifting armrest 2555 on the outside of the waterproof motor 254 facilitates the installation, disassembly and maintenance operations of the stirring component 25 as a whole. When the stirring component 25 needs to be inspected or replaced, it can be easily taken out of the stirring chamber 3 by pulling the armrest 2555, which improves the convenience and flexibility of operation.

[0038] See also Figure 3-Figure 5The cutting mechanism 9 includes a top cover 91 and a cutting box 92. The cutting box 92 is placed on the top of the digestion component 4. A driving motor 93 is fixedly connected to the upper end of one side of the top cover 91. An eccentric shaft 94 is fixedly installed at the output end of the driving motor 93 through the top cover 91. The side shape of the eccentric shaft 94 is concave. A linkage frame 95 is hinged on the outer side of the eccentric shaft 94. A top plate 96 is hinged on the outer side of the linkage frame 95. Rectangular cutting blades 97 are fixedly installed at equal intervals on the bottom of the top plate 96. A cutting net 98 is fixedly installed inside the cutting box 92. The mesh of the cutting net 98 is rectangular and its size corresponds to the rectangular cutting blade 97. Guide rails 99 are fixedly installed on both sides of the interior of the top cover 91, and a guide plate 910 is slidably connected to the inner side of the guide rail 99. The bottom of the guide plate 910 is fixedly connected to the outer side of the top plate 96. The driving motor 93 on one side of the top cover 91 provides power for the entire cutting process, ensuring the continuity and stability of the operation. Qualitatively, the eccentric shaft 94 is set in a concave shape, so that the movement of the linkage frame 95 can drive the top plate 96 and the rectangular cutting blade 97 to perform regular up and down and swinging movements. Such a design can achieve a more comprehensive and efficient cutting effect. The rectangular cutting blades 97 fixedly installed at equal intervals correspond to the rectangular mesh holes, and can accurately pass through the mesh holes during cutting to finely chop the materials, thereby ensuring the accuracy and uniformity of the cutting. The presence of the cutting net 98 can not only assist in cutting, but also screen and separate the cut materials. The guide rails 99 on both sides of the top cover 91 and the guide plates 910 slidably connected thereto provide a stable guide for the movement of the top plate 96, ensuring that the top plate 96 can move up and down and swing smoothly to avoid shaking or offset, thereby further improving the quality and accuracy of the cutting. The design of this cutting mechanism 9 is ingenious, and the synergistic effect of various components can efficiently complete the task of cutting materials.

[0039] See also Figure 3-Figure 5The bottom of the cutting box 92 and the digestion component 4 are fixedly connected with a sealing plug-in frame 911. The sealing plug-in frame 911 located at the bottom of the cutting box 92 is plugged into the inner upper end of the digestion component 4. The sealing plug-in frame 911 located on the digestion component 4 is plugged into the inner upper end of the box body 1. The outer surface of the sealing plug-in frame 911 is provided with a rubber film layer for improving the sealing performance. The plug-in connection of the sealing plug-in frame 911 ensures the tightness and stability of the connection between the cutting box 92 and the digestion component 4, and between the digestion component 4 and the box body 1, effectively preventing looseness or leakage during the working process, and ensuring the normal operation of the entire system. The rubber film layer further enhances the sealing performance, making the sealing The sealing effect is more reliable and can better prevent the leakage of liquid or other substances. This good sealing performance is conducive to maintaining the environmental stability within the system and ensuring the accuracy and reliability of various processing processes. The design of the sealing frame 911 also makes the assembly and disassembly between the parts relatively convenient and quick, which is convenient for equipment maintenance and inspection. When a part needs to be processed or replaced separately, it can be easily separated from other parts, which improves the flexibility and efficiency of operation. At the same time, the sealing frame 911 also plays a role in positioning and fixing to a certain extent, ensuring that each component is installed in the correct position, making the structure of the entire device more reasonable and stable.

[0040] See also Figure 3-Figure 5 The top cover 91, the cutting box 92 and the bottom of the digestion component 4 are all fixedly connected with a square sealing gasket 912, and the tops of the box body 1, the cutting box 92 and the digestion component 4 are all fixedly connected with a square sealing groove 913. The square sealing gasket 912 is inserted into the inner side of the square sealing groove 913. The coordinated use of the square sealing gasket 912 and the square sealing groove 913 provides all-round sealing protection. Compared with other shapes, the square design can provide a more uniform and comprehensive sealing effect on the sealing contact surface, reducing the occurrence of sealing dead corners. When the square sealing groove 913 is inserted into the square sealing groove 913, it can effectively prevent the leakage of liquid, gas or other substances at the connection parts of each component, ensuring the independence and stability of the internal environment of the system, which is very important for some processing processes with high sealing requirements, such as preventing the leakage of cell fluid, etc. This sealing structure also enhances the stability of the connection between the components, making the entire device more reliable during operation, and no unexpected situations will occur due to poor sealing. At the same time, this plug-in sealing method is simple and effective, easy to operate and maintain.

[0041] The working principle of the present invention is: during use, the umbilical cord segment is placed on the top of the cutting net 98, at this time, the top cover 91 is covered by starting the telescopic cylinder 6, and then the driving motor 93 is started to operate, the driving motor 93 drives the eccentric shaft 94 to rotate, and the rotation of the eccentric shaft 94 can drive the linkage frame 95 to telescope back and forth, and the telescopic frame pushes the top plate 96 to move up and down, and the guide rail 99 is slidably connected with a guide plate 910, and the guide plate 910 is connected to the top plate 96, and is guided by the guide plate 910 and the guide rail 99. The interface enables the eccentric activity force of the eccentric shaft 94 to act on the rectangular cutting blade 97 at the bottom of the top plate 96, and the rectangular cutting blade 97 is located in the mesh of the cutting net 98 at this time. The reciprocating movement displacement can enable the rectangular cutting blade 97 to reciprocate frequently to perform continuous cutting processing on the cutting blade, which can avoid the situation that the umbilical cord segment is not completely cut during cutting, and can improve the overall cutting stability of the device.

[0042] By setting up the centrifugal stirring mechanism 2, when the solution after cutting and digestion treatment is discharged into the stirring chamber 3 during use of the device, the centrifugal solution can be added into the stirring chamber 3 through the solution feeding hopper 12 in cooperation with the solution feeding pipe 11. The bottom of the solution feeding hopper 12 is provided with a regulating valve, which can be closed to seal the bottom of the solution feeding hopper 12 after the solution is added, so as to facilitate the sealing treatment. By starting the waterproof motor 254 to run, the waterproof motor 254 can drive the inner shaft 2551 located inside the filter cover 24 to rotate, and the inner shaft 2551 The rotation can drive the L-shaped stirring rod 2552 and the straight stirring rod 2553 to rotate. The rotation of the L-shaped stirring rod 2552 and the straight stirring rod 2553 can assist in stirring the inner side of the filter cover 24, thereby promoting the solution and umbilical cord stem cells to be fully mixed and centrifuged. At the same time, during this process, the stirring paddle 2554 arranged in a spiral auger shape can assist in further tumbling the solution and stem cell material, allowing the solution and material to be mixed more evenly, thereby improving the quality of its centrifugal mixing, improving its overall performance, and improving the efficiency of pretreatment of human umbilical cord stem cells.

[0043] By setting up the centrifugal stirring mechanism 2, when the device is in use, if it is necessary to discharge the solution, the external solenoid valve 10 can be directly opened. When the external solenoid valve 10 is opened, the solution can be discharged through the external solenoid valve 10. At the same time, during this period, the stem cells in the solution can be filtered and screened by the microporous filter membrane and cell screen inside the filter cover 24, which can cause the stem cells to be retained on the inner side of the filter cover 24. When the waste water is discharged, the internal solenoid valve 26 can be opened, and the waterproof motor 254 can be started to drive the stirring paddle 2554 to rotate. The stirring paddle 2554 is spirally arranged, which can spirally squeeze the remaining purified stem cells to surge downward through the internal solenoid valve 26 and be discharged into The interior of the external solenoid valve 10 is convenient for quickly discharging the umbilical cord solution, which can improve the convenience of the overall device for taking and placing the purified umbilical cord stem cells. When cleaning is required, it is only necessary to start the telescopic cylinder 6 to drive the top cover 91 to separate from the top of the cutting box 92. At this time, the cutting box 92 and the digestion component 4 are adaptively taken out, and then the support tube 21 is pulled out to pull the entire centrifugal stirring mechanism 2 out of the interior of the box body 1, and finally the plug 252 is pulled out from the inside of the socket 251 to separate the stirring frame 255 and the filter cover 24. At this time, the stirring chamber 3, the stirring frame 255, the filter cover 24, the digestion component 4 and the entire box body 1 are convenient for subsequent cleaning, inspection and maintenance, which can improve the overall convenience of use of the device.

Claims

1. A pretreatment device for culturing human umbilical cord mesenchymal stem cells, characterized in that: The invention comprises a box body (1), wherein a centrifugal stirring mechanism (2) is fixedly installed at the lower end of the box body (1), a stirring chamber (3) is provided inside the box body (1), the bottom of the centrifugal stirring mechanism (2) extends into the interior of the stirring chamber (3), a digestion assembly (4) is placed on the top of the box body (1), a mounting seat (5) is fixedly installed in the middle of the back of the box body (1), a telescopic cylinder (6) is fixedly installed on the inner side of the mounting seat (5), a connecting rod (7) is fixedly installed at the top output end of the telescopic cylinder (6) passing through the mounting seat (5), a top plate (8) is fixedly installed on the top of the connecting rod (7), a cutting mechanism (9) is fixedly installed on the front of the top plate (8), and the cutting mechanism (9) covers the top of the digestion assembly (4), an external electromagnetic valve (10) is fixedly installed in the middle of the bottom of the box body (1), and the input end of the external electromagnetic valve (10) is connected to the bottom of the box body (1); The centrifugal stirring mechanism (2) comprises a support tube (21), an insertion rod (22) is inserted into the interior of the support tube (21), a sealing disk (23) is fixedly mounted on the top of the insertion rod (22), a filter cover (24) is fixedly mounted on the bottom of the sealing disk (23), and a stirring assembly (25) is movably mounted on the top of the sealing disk (23); The stirring assembly (25) comprises a socket (251), wherein the socket (251) is provided at four corners of the top of the sealing disk (23), the inside of the socket (251) is fixedly connected to a plug post (252), the outer surface of the plug post (252) is fixedly connected to a layer of sealing rubber sleeve, an inner bracket (253) is fixedly installed between the inner sides of the plug post (252), a waterproof motor (254) is fixedly installed in the middle of the top of the inner bracket (253), and a stirring frame (255) is fixedly installed at the output end of the waterproof motor (254) through the inner bracket (253); The stirring frame (255) comprises an inner shaft (2551), the inner shaft (2551) is fixedly mounted on the bottom output end of the waterproof motor (254), an L-shaped stirring rod (2552) is fixedly mounted at an equal interval on the outer upper end of the inner shaft (2551), and a straight stirring rod (2553) is fixedly mounted at an equal interval on the inner side of the L-shaped stirring rod (2552); A stirring paddle (2554) is fixedly mounted on the bottom of the inner shaft (2551); the stirring paddle (2554) is arranged in a spiral auger shape; the lower end of the stirring paddle (2554) is also arranged in a conical shape; the bottom of the stirring paddle (2554) is fittedly connected to the inner bottom of the box body (1); and a lifting armrest (2555) is fixedly connected to the outer side of the waterproof motor (254); The cutting mechanism (9) comprises a top cover (91) and a cutting box (92), wherein the cutting box (92) is placed on the top of the digestion component (4), and a driving motor (93) is fixedly connected to an upper end of one side of the top cover (91), and an eccentric shaft (94) is fixedly installed on the output end of the driving motor (93) through the top cover (91), and the side surface of the eccentric shaft (94) is in a concave shape, and a linkage frame (95) is hingedly connected to the outer side of the eccentric shaft (94), and a top plate is hingedly connected to the outer side of the linkage frame (95). (96), rectangular cutting blades (97) are fixedly installed at equal intervals on the bottom of the top plate (96), a cutting net (98) is fixedly installed inside the cutting box (92), the mesh of the cutting net (98) is arranged in a rectangular shape and the size corresponds to the rectangular cutting blades (97), guide rails (99) are fixedly installed on both sides of the interior of the top cover (91) at both front and rear ends, the inner side of the guide rail (99) is slidably connected to a guide plate (910), and the bottom of the guide plate (910) is fixedly connected to the outer side of the top plate (96); The bottoms of the cutting box (92) and the digestion assembly (4) are both fixedly connected with a sealing insert frame (911); the sealing insert frame (911) located at the bottom of the cutting box (92) is plugged into the inner upper end of the digestion assembly (4); the sealing insert frame (911) located on the digestion assembly (4) is plugged into the inner upper end of the box body (1); and the outer surface of the sealing insert frame (911) is provided with a rubber film layer for improving the sealing performance; The top cover (91), the cutting box (92) and the bottom of the digestion component (4) are all fixedly connected with a square sealing gasket (912); the tops of the box body (1), the cutting box (92) and the digestion component (4) are all fixedly connected with a square sealing groove (913); and the square sealing gasket (912) is inserted into the inner side of the square sealing groove (913).

2. A pretreatment device for culturing human umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The support tube (21) is fixedly installed at the four corners of the box body (1); the filter cover (24) is composed of a stainless steel mesh cover; a layer of cell screen is fixedly connected to the inside of the filter cover (24); a layer of microporous filter membrane is also fixedly connected to the inner side of the cell screen in the filter cover (24); an internal solenoid valve (26) is fixedly installed at the bottom of the filter cover (24); a discharge pipe (27) is fixedly connected to the bottom of the internal solenoid valve (26); and the lower end of the discharge pipe (27) extends to the input end of the external solenoid valve (10).

3. A pretreatment device for culturing human umbilical cord mesenchymal stem cells according to claim 1, characterized in that: A solution feeding pipe (11) is fixedly mounted on the upper end of one side of the box body (1), a solution feeding hopper (12) is fixedly mounted on the top of the solution feeding pipe (11), supporting legs (13) are fixedly mounted at the four corners of the bottom of the box body (1), and a non-slip bottom plate is fixedly mounted on the bottom of the supporting legs (13).

4. A pretreatment device for culturing human umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The upper end of the interior of the stirring chamber (3) is in a rectangular shape, the lower end of the interior of the stirring chamber (3) is in a cylindrical shape, and the bottom of the interior of the stirring chamber (3) is in a conical shape.

Images