Lactobacillus plantarum MH95 with antioxidant, anti-aging and anti-photoaging effects and application thereof

Abstract

The present invention relates to a plant lactobacillus MH95 having antioxidant, anti-aging and anti-photoaging effects. The plant lactobacillus MH95 is classified as plant lactobacillus Lactiplantibacillus plantarum , the deposit number is CGMCC No.31053, and the deposit date is June 24, 2024. The bacterium has excellent in vitro antioxidant properties, specifically manifested in excellent scavenging rate and iron reduction ability for DPPH free radicals and OH free radicals; it can improve ultraviolet-induced skin photoaging, specifically manifested in regulating superoxide dismutase activity and the content of malondialdehyde and collagen; it can also improve the proliferation ability of human skin fibroblasts.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms and relates to Lactobacillus plantarum MH95 with anti-oxidation, anti-aging and anti-photoaging effects and application thereof. Background Art

[0002] With the improvement of living standards and the aging of the population, people's demand for anti-aging and anti-photoaging is increasing. Skin aging is a biological phenomenon, including natural aging in the body and aging caused by external factors. Ultraviolet radiation can cause photoaging of human skin and is the most important external environmental factor. It not only affects the appearance of the skin, but also accelerates the damage and aging process of skin cells.

[0003] During skin aging and photoaging, two key biomarkers are widely used to assess the degree of oxidative stress: superoxide dismutase (SOD) and malondialdehyde (MDA). Superoxide dismutase is an important antioxidant enzyme in cells, responsible for scavenging superoxide anion free radicals, thereby protecting cells from oxidative damage. However, with the progression of photoaging, the activity of superoxide dismutase decreases significantly, weakening the ability of skin cells to resist oxidative stress. In contrast, malondialdehyde is a product of lipid peroxidation, and its increased level reflects the degree of oxidative damage to cell membrane lipids. The increased level of malondialdehyde caused by photoaging is an important sign of skin cell damage and enhanced inflammatory response.

[0004] In addition, photoaging also has a significant effect on the ability of skin fibroblasts to synthesize collagen. Collagen is the main component of the skin matrix. Hydroxyproline (HYP) is the amino acid with the highest content in collagen. The determination of hydroxyproline can be used to understand the situation of collagen in the body. It is responsible for maintaining the structure and elasticity of the skin. Under photoaging conditions, excessive production of reactive oxygen species inhibits the synthesis of collagen in fibroblasts and activates matrix metalloproteinases (MMPs), which degrade existing collagen, further leading to skin sagging and wrinkle formation.

[0005] At present, there are various strategies for anti-oxidation, anti-aging and anti-photoaging, mainly including the application of chemical antioxidants, physical barrier protection and cell repair technology. Among them, antioxidants such as vitamin C, vitamin E, coenzyme Q10, etc., slow down the photoaging process by neutralizing free radicals. Physical protection, such as the use of broad-spectrum sunscreen, can effectively block ultraviolet rays and reduce skin damage. In addition, cell repair technologies such as gene editing and stem cell therapy have also shown potential in anti-aging research. These strategies provide multi-level protection for delaying aging. However, long-term use of high-concentration antioxidants or sunscreens may cause skin irritation or allergic reactions, increase skin sensitivity, and pose potential hazards to the skin or human body. The research and application of cell repair technology is costly, involving complex experiments and high-end equipment, making it difficult to promote on a large scale.

[0006] Therefore, developing a more natural and safe product with antioxidant, anti-aging and anti-photoaging effects not only meets market demand, but also provides important support for improving people's quality of life. Summary of the invention

[0007] In view of the deficiencies in the prior art, the object of the present invention is to provide a Lactobacillus plantarum MH95 with antioxidant, anti-aging and anti-photoaging effects and applications thereof.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In the first aspect, the present invention provides a plant lactobacillus MH95 having antioxidant, anti-aging and anti-photoaging effects, wherein the plant lactobacillus MH95 is classified as plant lactobacillus Lactiplantibacillus plantarum The deposit number is CGMCC No.31053, and the deposit date is June 24, 2024.

[0010] The present invention isolates and preserves a new plant lactobacillus MH95 with antioxidant, anti-aging and anti-photoaging effects from a grape juice fermentation product sample in Xinjiang Uygur Autonomous Region. The strain is sequenced and analyzed, and its 16SrDNA sequence is shown in SEQ ID No: 1. The sequence obtained by sequencing is compared with the nucleic acid sequence in GeneBank, and the result shows that the strain is plant lactobacillus. The bacterium has excellent in vitro antioxidant performance, which is specifically manifested in excellent scavenging rate and iron reducing ability (FRAP) for DPPH free radicals and OH free radicals; it can improve ultraviolet-induced skin photoaging, which is specifically manifested in regulating superoxide dismutase activity and malondialdehyde and collagen content; and it can also improve the proliferation ability of human skin fibroblasts. It has a promising prospect for using it to prepare products with antioxidant, anti-aging and anti-photoaging effects.

[0011] In a second aspect, the present invention provides a use of the Lactobacillus plantarum MH95 described in the first aspect in preparing a product having antioxidant, anti-aging and anti-photoaging effects.

[0012] Preferably, the product includes medicine, food, health care product, daily chemical product or household textile.

[0013] Preferably, the product scavenges free radicals and enhances iron reducing capacity.

[0014] The product regulates the activity of superoxide dismutase and the content of malondialdehyde and collagen.

[0015] The product promotes the proliferation of skin fibroblasts.

[0016] Preferably, the bacterial count of Lactobacillus plantarum MH95 in the product is not less than 1×10 8 / mL or 1×10 8 Pieces / g, for example 1×10 8 pieces / g (pieces / mL), 1×10 9 pieces / g (pieces / mL), 1×10 10 pieces / g (pieces / mL), 5×10 10 pieces / g (pieces / mL), 1×10 11 pieces / g (pieces / mL), 3×10 11 pieces / g (pieces / mL), 5×10 11 pieces / g (pieces / mL), 1×10 12 Pieces / g (pieces / mL), etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0017] Preferably, the bacterial count of Lactobacillus plantarum MH95 in the product is not higher than 1×10 13 CFU / mL or 1×10 13 CFU / g.

[0018] In the above products, Lactobacillus plantarum MH95 can exist in both a live bacteria state and an inactivated state.

[0019] In a third aspect, the present invention provides a microbial preparation having antioxidant, anti-aging and anti-photoaging effects, wherein the strain in the microbial preparation includes the Lactobacillus plantarum MH95 described in the first aspect.

[0020] Preferably, the strain in the microbial preparation also includes Lactobacillus salivarius LS97; the classification name of Lactobacillus salivarius LS97 is Lactobacillus salivarius Lactobacillus salivarius, deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, with the deposit number CGMCC No.16922 and the deposit date of December 10, 2018.

[0021] The present invention also creatively discovered that the above-mentioned Lactobacillus plantarum MH95 strain can be used in combination with Lactobacillus salivarius LS97 strain for anti-oxidation, anti-photoaging and anti-aging, and also has excellent effects. Compared with a single bacterial agent, the composite bacterial agent has more significant antioxidant, anti-photoaging and anti-aging effects, indicating that the MH95 strain and the LS97 strain have potential synergistic effects in the above-mentioned effects.

[0022] Preferably, the ratio of the bacterial counts of Lactobacillus plantarum MH95 to Lactobacillus salivarius LS97 is (2-10):1, for example 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, etc. Other specific point values ​​within this numerical range can be selected and will not be described one by one here.

[0023] Based on the potential interaction between the two strains in terms of anti-oxidation, anti-photoaging and anti-aging, the effect will be better when the two meet the above-mentioned specific ratio relationship.

[0024] Preferably, the total bacterial count in the microbial preparation is not less than 1×10 8 / g or 1×10 8 Pieces / mL.

[0025] Preferably, the total bacterial count in the microbial preparation is not higher than 1×10 13 CFU / mL or 1×10 13 CFU / g.

[0026] Preferably, the microbial preparation is in the form of a solution or a powder.

[0027] The dosage form of the probiotics involved in the present invention is not limited, including the most commonly used solutions, powders, or further prepared capsules, tablets or granules.

[0028] In a fourth aspect, the present invention provides the use of the microbial preparation described in the third aspect in preparing a product having any one or at least two of the following effects:

[0029] 1) Anti-oxidation; 2) Anti-aging; 3) Anti-photoaging.

[0030] In the above products, Lactobacillus plantarum MH95 or Lactobacillus salivarius LS97 can exist in either a live bacteria state or an inactivated state.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention isolates and preserves a new plant lactobacillus MH95 with antioxidant, anti-aging and anti-photoaging effects from a grape juice fermentation product sample in Xinjiang Uygur Autonomous Region. The strain is sequenced and analyzed, and its 16SrDNA sequence is shown in SEQ ID No: 1. The sequence obtained by sequencing is compared with the nucleic acid sequence in GeneBank, and the result shows that the strain is plant lactobacillus. The bacterium has excellent in vitro antioxidant performance, which is specifically manifested in excellent scavenging rate and iron reducing ability (FRAP) for DPPH free radicals and OH free radicals; it can improve ultraviolet-induced skin photoaging, which is specifically manifested in regulating superoxide dismutase activity and malondialdehyde and collagen content; and it can also improve the proliferation ability of human skin fibroblasts. It has a promising prospect for using it to prepare products with antioxidant, anti-aging and anti-photoaging effects.

[0033] The MH95 strain involved in the present invention is classified and named as Lactobacillus plantarum Lactiplantibacillus plantarum The depository is the General Microbiology Center of China Microbiological Culture Collection Administration. The deposit date is June 24, 2024. The deposit number is CGMCC No.31053. The address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0034] The LS97 strain involved in the present invention is classified and named as Lactobacillus salivarius Lactobacillus salivarius The depository is the General Microbiology Center of China Microbiological Culture Collection Administration. The deposit date is December 10, 2018. The deposit number is CGMCC No.16922. The address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a microscopic image of the Lactobacillus plantarum MH95 strain. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0037] The mouse fibroblasts (NIH-3T3) and human skin fibroblasts (HDFa) involved in the following contents were purchased from Nanjing Kebai Biotechnology Co., Ltd., and the experimental mice were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd.

[0038] The culture media involved in the following content are as follows:

[0039] MRS liquid culture medium: glucose 20 g / L, yeast peptone 10 g / L, beef extract powder 10 g / L, yeast extract powder 5 g / L, anhydrous sodium acetate 5 g / L, Tween-80 1 g / L, diammonium hydrogen citrate 2 g / L, dipotassium hydrogen phosphate 2 g / L, magnesium sulfate heptahydrate 0.58 g / L, manganese sulfate monohydrate 0.19 g / L, water 1000 mL, pH 6.8.

[0040] The MH95 strain referred to below is named Lactobacillus plantarum Lactiplantibacillus plantarum The depository is the General Microbiology Center of China Microbiological Culture Collection Administration. The deposit date is June 24, 2024. The deposit number is CGMCC No.31053. The address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0041] The LS97 strains referred to below are designated as Lactobacillus salivarius Lactobacillus salivarius The depository is the General Microbiology Center of China Microbiological Culture Collection Administration. The deposit date is December 10, 2018. The deposit number is CGMCC No.16922. The address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0042] The inactivated bacterial suspension involved below is obtained as follows: the seed solution of the desired strain is inoculated into the MRS medium at an inoculation amount of 3% of the total mass of the medium, and cultured at 37°C to obtain a bacterial suspension; the bacterial suspension is transferred to a sterile centrifuge tube, the precipitate is collected by centrifugation, and resuspended with a sterile PBS buffer solution, and the centrifugation and resuspension are repeated twice to remove the residue in the medium; the bacterial suspension is heat-inactivated at 90°C for 30 min. Example 1

[0043] Isolation and screening of Lactobacillus plantarum MH95:

[0044] (1) Preliminary screening: The grape juice fermentation product samples collected from Xinjiang Uygur Autonomous Region were diluted tenfold with sterile physiological saline. The diluted samples were centrifuged at 3000 rpm for 10 min, and the supernatant was collected and filtered with a sterile filter to further remove suspended impurities. The supernatant was spread on a MRS solid culture medium (containing hydrogen peroxide) plate and cultured for 72 h under anaerobic conditions at 37±1℃. The strains with better growth on the plate were selected.

[0045] (2) Rescreening: The above strains are rescreened using the in vitro antioxidant performance index as the rescreening condition. The specific in vitro antioxidant performance indexes are: scavenging rate of DPPH free radicals, scavenging rate of OH free radicals and iron reducing ability (FRAP), which are carried out using conventional methods in the field. The specific operation method is as follows:

[0046] ①1 mL of different bacterial solutions were taken and added to 0.2 mmol / L DPPH solution in equal volumes for reaction for 30 min. The absorbance was measured at 571 nm by UV spectrophotometer and the DPPH radical scavenging rate was calculated. ②1 mL of different bacterial solutions were taken and added to equal volumes of ferrous sulfate solution, salicylic acid solution and H2O2 solution respectively. After reacting in a water bath at 37℃ for 30 min, the absorbance was measured at 510 nm and the OH radical scavenging rate was calculated. ③1 mL of different bacterial solutions were taken and added to equal volumes of PBS buffer solution and K3[Fe(CN)6] solution. After reacting in a water bath at 50℃ for 20 min, the solution was taken out. An equal amount of C2HCl3O2 solution was added and centrifuged for 10 min. Then 1 mL of supernatant was taken and added to equal volumes of distilled water and FeCl3 solution for reaction for 10 min. The absorbance was measured at 700 nm and the reducing ability values ​​were compared. A strain with the highest scavenging rate for DPPH free radicals and OH free radicals and the strongest iron reduction ability was screened out and named MH95 strain. Example 2

[0047] Identification of Lactobacillus plantarum MH95:

[0048] (1) Observe the screened MH95 strain under a microscope. Figure 1 As shown, the strains are rod-shaped and arranged singly, in pairs or in short chains.

[0049] (2) Carbon source metabolism experiments were conducted on the screened MH95 strain (using the API 50 CH microbial biochemical identification system). The results are shown in Table 1. The MH95 strain is able to utilize a variety of sugars, such as glucose, fructose, lactose, maltose and sucrose.

[0050] Table 1

[0051]

[0052] (3) 16S rDNA molecular biological identification:

[0053] The screened MH95 strain was inoculated in a centrifuge tube containing 20 mL MRS liquid medium at a ratio of 2%, and centrifuged at 37 ° C for 18 h, centrifuged at 5000 rpm for 10 min, and the supernatant was removed to collect the bacteria. The genome of the strain was extracted, and bacterial universal primers were added for PCR amplification, and the amplified product was sequenced and identified. The strain was sequenced and analyzed, and its 16S rDNA sequence was shown in SEQ ID No: 1. The sequence obtained by sequencing was compared with the nucleic acid sequence in GeneBank, and the result showed that the strain was plant lactobacillus. Example 3

[0054] Safety evaluation test:

[0055] Mouse fibroblasts NIH-3T3 in the logarithmic phase were collected, 100 μL of culture medium was added, and then cultured in a 96-well plate. The concentration of the cell suspension was adjusted to 10 cells per well. 4 cells; after observing that the cells are completely attached to the wall, add different concentrations of inactivated Lactobacillus plantarum MH95 suspension or inactivated Lactobacillus salivarius LS97 suspension as the sample group, add culture medium as the sample control group, and transfer the 96-well plate to 37°C for 24 hours; centrifuge to remove the culture medium, add 20 μL of 0.5% MTT solution to each well and culture for 1 day; after removing the supernatant, add 150 μL of DMSO solution and shake, observe that the blue-purple formazan crystals are completely dissolved, and measure the absorbance with an enzyme marker at a wavelength of 490nm. Set three parallel groups for each concentration, and use the wells without bacterial suspension and culture medium as the blank group. The cell survival rate formula is as follows:

[0056] Cell survival rate = (A i -A0) / (A j -A0)×100%

[0057] Where: A0 is the absorbance of the blank group; A i is the absorbance of the sample group; A j is the absorbance of the sample control group.

[0058] The results showed that the concentration of Lactobacillus plantarum MH95 or Lactobacillus salivarius LS97 was less than 1×10 13 When the concentration of 50 μg / mL was 400 μg / mL, the cell survival rate was above 88%, and it had no cytotoxicity to mouse fibroblast NIH-3T3 cells. Example 4

[0059] Anti-light aging effect evaluation test:

[0060] (1) Experimental groups:

[0061] Forty-eight 8-week-old Kunming mice, half male and half female, were divided into 8 groups, with 6 mice in each group; namely: blank group, model group, MH95 group, LS97 group, MH95+LS97 group 1 (the ratio of bacterial count was 2:1), MH95+LS97 group 2 (the ratio of bacterial count was 10:1), commercially available Lactobacillus plantarum+LS97 group (the ratio of bacterial count was 2:1, the commercially available Lactobacillus plantarum was ATCC8014), and positive control group.

[0062] (2) Model establishment and intervention methods:

[0063] The hair on the back of the mice was shaved with an electric hair clipper to fully expose the skin on the back, with an area of ​​about 2 cm × 2 cm. The experiment began on the second day after hair removal.

[0064] In the blank group, the mice's limbs were fixed on a fixing frame, and 50 μL of pure water was evenly applied to the exposed skin once a day.

[0065] The model group had their limbs fixed on a fixture and UVB irradiated the exposed part of the back skin of the mice. The UVB irradiation dose was: 0.054 J / cm2 each time on Mondays, Wednesdays, and Fridays in the first week. 2 , 0.108 J / cm each time in the second week 2 , 0.200 J / cm each time in the third and fourth weeks 2 During the irradiation process, the mouse's head was covered with a paper sheet.

[0066] The mice in the probiotic intervention group were fixed in the same way, and 50 μL of bacterial suspension samples (single bacterial suspension or mixed bacterial suspension, the total bacterial concentration of each group was 1×10 12 / mL), and then give UVB irradiation.

[0067] Mice in the positive control group were fixed in the same way, and 50 μL of vitamin E solution (concentration of 5 μg / mL) was evenly applied to the exposed skin half an hour before irradiation.

[0068] (3) Skin sampling:

[0069] The mice were killed by vertebral dislocation 24 days after the treatment, and 0.5 g of skin tissue from the back of the neck was immediately taken. After rinsing with precooled saline, excess fat and other connective tissue were removed, and a 10% skin tissue homogenate was made. The supernatant of the homogenate was taken to measure the indicators.

[0070] (4) Indicator analysis:

[0071] The superoxide dismutase activity (SOD), malondialdehyde (MDA) content and hydroxyproline (HYP) content in the skin tissue homogenate supernatant were determined using a kit. The results are shown in Table 2.

[0072] Table 2

[0073]

[0074] It can be seen from the data results in Table 2 that compared with the blank group, the superoxide dismutase activity and hydroxyproline content in the skin tissue of the mice in the model group were reduced, and the malondialdehyde content was increased. After the intervention of each group of strains, the superoxide dismutase activity and hydroxyproline content increased to varying degrees, and the malondialdehyde content decreased to varying degrees, and the effects of MH95+LS97 groups 1 and 2 were better, indicating that the microbial preparation involved in the present invention can improve ultraviolet-induced skin photoaging. Example 5

[0075] In vitro proliferation effect evaluation test of human skin fibroblasts:

[0076] (1) Experimental groups:

[0077] In order to detect the effect of samples on cell aging, bacterial solution was added to the culture medium of human skin fibroblasts (HDFa) in vitro for subculture, and the number of days between subcultures in each group was recorded. The experiment was divided into a blank control group (not treated with probiotic solution), MH95 group, LS97 group, MH95 + LS97 group 1 (the ratio of bacterial count was 2:1), MH95 + LS97 group 2 (the ratio of bacterial count was 10:1), commercially available plant lactobacillus + LS97 group (the ratio of bacterial count was 2:1, and the commercially available plant lactobacillus was ATCC8014), and a positive control group (containing 5% Fukangle liquid).

[0078] (2) Cellular intervention methods:

[0079] Fibroblasts in the logarithmic phase were collected and seeded into 6-well plates, with 2×10 5 The cells were treated after reaching 80-90% confluence, and the cells were cultured with the corresponding culture medium of each group. The control group used DMEM culture medium, the positive control group used DMEM culture medium supplemented with 5% Fukangle (a commercially available anti-aging drug), and each strain intervention group used DMEM culture medium supplemented with different bacterial suspensions (the final bacterial concentration in the culture system was 1×10 12 / mL).

[0080] (3) Generation records:

[0081] Whenever cells reached 80-90% confluence, the interval between passages was recorded (time from seeding to reaching 80-90% confluence). The cells were digested and replated at a 1:2 ratio, and the interval between passages was recorded. This was continued for 14 passages (P1-P 14 ) to observe the changes in the passage interval between groups. The results are shown in Table 3.

[0082] Table 3

[0083]

[0084] Note: “-” means the cells have entered the senescence phase and stopped proliferating.

[0085] From the data results in Table 3, it can be seen that the interval time of cell subculture in each group and before the 8th time is exactly the same. Compared with the blank control group, the upward trend of the cell subculture time interval in each intervention group is accelerated, and each interval time after the 8th subculture is significantly lower than that of the cells in the blank control group. The cell proliferation rate is improved to varying degrees, and the effects of MH95+LS97 groups 1 and 2 are better, and there is no significant difference, indicating that the microbial preparation involved in the present invention can promote the proliferation ability of skin fibroblasts.

[0086] (4) Cell proliferation detection:

[0087] MTT method was used to detect the blank control group and the strain intervention group (the final bacterial concentration in the culture system was 1×10 8 The 0th and 7th generation human skin fibroblasts (100 μg / mL) were inoculated into 96-well plates, with 3 replicate wells in each group. They were placed in a cell culture incubator for 24 h, the culture medium was removed by centrifugation, and a 0.5% MTT solution was added to each well and incubated at 37°C for 4 h. After removing the supernatant, 150 μL DMSO was added and shaken vigorously for 10 min in the dark, and the absorbance of the solution at 570 nm was tested using an ELISA reader. Each group of experiments was repeated 3 times and the data were combined. The average proliferation rate was calculated by comparing the absorbance of the 0th and 7th generation cells. The results are shown in Table 4.

[0088] The formula for the proliferation rate is as follows: Y (%) = [A P7 / A P0 ]×100%

[0089] Table 4

[0090]

[0091] It can be seen from the data results in Table 4 that after the cells in the blank control group were cultured to the P7 generation, their proliferation ability was only about 50% of that of the P0 generation, and the proliferation ability was significantly reduced; while the proliferation ability of the cells in the intervention group was significantly stronger when they were cultured to the P7 generation, among which the effects of MH95+LS97 groups 1 and 2 were better, indicating that the microbial preparation involved in the present invention has the ability to promote the proliferation of skin fibroblasts, and has excellent effects of promoting skin metabolism and anti-aging.

[0092] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0093] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0094] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A microbial preparation having antioxidant, anti-aging and anti-photoaging effects, characterized in that: The strains in the microbial preparation are Lactobacillus plantarum MH95 and Lactobacillus salivarius LS97; The classification of the plant lactobacillus MH95 is named plant lactobacillus Lactiplantibacillus plantarum , deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, with the deposit number CGMCC No.31053 and the deposit date June 24, 2024; The classification of the Lactobacillus salivarius LS97 is named Lactobacillus salivarius Lactobacillus salivarius , deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, with the deposit number CGMCC No.16922 and the deposit date being December 10, 2018.

2. The microbial preparation according to claim 1, characterized in that The ratio of the bacterial counts of Lactobacillus plantarum MH95 and Lactobacillus salivarius LS97 is (2-10):

1.

3. The microbial preparation according to claim 1, characterized in that The total bacterial count in the microbial preparation is not less than 1×10 8 / mL or 1×10 8 Pieces / g.

4. The microbial preparation according to claim 1, characterized in that The dosage form of the microbial preparation includes solution or powder.

5. Use of the microbial preparation according to any one of claims 1 to 4 in the preparation of a product having any one or at least two of the following effects: 1) Anti-oxidation; 2) Anti-aging; 3) Anti-photoaging.

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