A ganoderma lucidum strain culture screening device and method
By designing a culture screening device for Ganoderma lucidum bacterial seed culture and screening device, using a water bath heating system and a stable clamping plate, the problem of inability to effectively regulate the temperature of the culture medium in the prior art is solved, the survival rate and growth efficiency of the bacterial seeds are improved, and the practicality of the culture is enhanced. sex.
Patent Information
- Application Number
- CN202410828409.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-25
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2044-06-25
AI Technical Summary
The existing Ganoderma lucidum strain culture device cannot effectively regulate the temperature of the culture medium, which affects the survival rate and growth efficiency of the strain, and thus affects the practicality of the culture.
A Ganoderma lucidum culture screening device is designed, including a culture main cabinet and a water bath heating system. The constant temperature control of the culture medium is achieved by heating the agitating plate and the water bath electric heating wire, and the culture box is ensured by a stable clamping plate and a lifting synchronous motor. stability.
By ensuring the constant temperature control of the culture medium and the stability of the culture box, the survival rate and growth efficiency of Ganoderma lucidum species are improved, and the practicality of culture is enhanced.
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Figure CN118765721B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of ganoderma lucidum cultivation, and in particular relates to a ganoderma lucidum strain cultivation and screening device and method thereof. Background Art
[0002] Through artificial selection and modern biotechnology, such as mutagenesis, hybrid breeding, protoplast fusion technology and genetic engineering breeding, Ganoderma lucidum strains with excellent characteristics can be bred. These strains usually have the characteristics of vigorous mycelial growth, strong adaptability, and strong resistance to adverse external environments, thereby ensuring the yield and quality of Ganoderma lucidum. Under appropriate culture conditions, such as temperature, humidity, light, etc., Ganoderma lucidum strains can grow and reproduce stably, thereby ensuring the stability of Ganoderma lucidum's yield and quality. Through Ganoderma lucidum strain cultivation, the uncertainty brought about by directly collecting strains from wild Ganoderma lucidum can be avoided, such as inconsistent strain quality and susceptibility to diseases and pests, thereby reducing cultivation risks. Ganoderma lucidum strain cultivation provides a large number of high-quality strains for the Ganoderma lucidum industry, which is conducive to large-scale production and market promotion of the Ganoderma lucidum industry.
[0003] The patent with application number 202322697284.1 discloses a biological safety cabinet for cultivating red Ganoderma lucidum strains. The application can adjust the distance between each culture rack assembly to adapt to the size of different red Ganoderma lucidum strain culture bags, avoid the situation that the red Ganoderma lucidum strains are crushed, and also set an air intake assembly with a filtering effect to supply clean fresh air to the inside of the cabinet; in combination with the above application and in connection with the existing culture device, during the culture process, the culture substrate needs to be placed in the safety cabinet to play a certain protective role on the Ganoderma lucidum culture substrate and ensure the stability of the Ganoderma lucidum growth environment. However, the existing safety cabinet controls the temperature to be constant through the air-conditioning system. The air-conditioning system can only ensure the temperature of the air environment and cannot regulate the temperature of the culture substrate, which will have a certain impact during the culture process, affecting the survival rate of the strains while affecting the growth efficiency of the strains, thereby affecting the practicability of the culture. For this reason, we propose a Ganoderma lucidum strain culture screening device and method. Summary of the invention
[0004] The purpose of the present invention is to provide a Ganoderma lucidum strain culture screening device and method thereof, so as to solve the problem that in the existing culture device proposed in the above background technology, the culture substrate needs to be placed in a safety cabinet during the culture process to protect the Ganoderma lucidum culture substrate to a certain extent and ensure the stability of the Ganoderma lucidum growth environment. However, the existing safety cabinet controls the temperature to be constant through an air-conditioning system. The air-conditioning system can only ensure the temperature of the air environment and cannot regulate the temperature of the culture substrate, which will have a certain impact during the culture process, affecting the survival rate of the strain and the growth efficiency of the strain, thereby affecting the practicability of the culture.
[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a Ganoderma lucidum strain culture and screening device, comprising a culture main body cabinet, a workbench is installed on the front surface of the culture main body cabinet, a culture main body trough is installed on the inner side of the workbench, a culture box is placed in the culture main body trough, a box body lifting and placing plate that can be raised and lowered is arranged on the inner side of the culture main body trough, the culture box is placed on the box body lifting and placing plate that can be raised and lowered, a stable clamping plate for clamping the culture box is arranged on the inner top of the culture main body trough, and a water bath heating stirring plate for rotating and stirring water flow is also installed on the inner side of the culture main body trough, and a water bath heating ring for heating is arranged at the edge of the water bath heating stirring plate located in the culture main body trough.
[0006] Preferably, a lifting synchronous motor is symmetrically installed at the bottom of the workbench, a lifting screw is installed at the top of the lifting synchronous motor located in the culture main body groove, and a lifting limit seat is provided on one side of the box body lifting placement plate.
[0007] Preferably, the lifting screw passes through the lifting limit seat, and a lifting limit groove is opened at the edge of the culture main body groove, and the box body lifting and placing plate is limited and lifted in the lifting limit groove by the lifting limit seat and the lifting screw. At the same time, two groups of box body lifting and placing plates are symmetrically arranged at both ends of the inner side of the culture main body groove, and the surface of the box body lifting and placing plate is opened with a through liquid through hole.
[0008] Preferably, the bottom of the workbench is located directly below the culture main body tank and is equipped with a stirring motor, the top of the stirring motor is located in the culture main body tank and is equipped with a water bath heating stirring plate, and the water bath heating stirring plate is provided with stirring protrusions.
[0009] Preferably, a water bath electric heating wire is arranged inside the water bath heating ring, and both the water bath heating ring and the water bath electric heating wire are annular structures.
[0010] Preferably, a limited clamping groove is provided at the inner top of the culture main body groove, a limited clamping guide rod is installed on the inner side of the limited clamping groove, a clamping spring is installed on the limited clamping guide rod, and a stable clamping plate is also installed on the inner side of the culture main body groove.
[0011] Preferably, a rubber clamping gasket is provided on one side of the stabilizing clamping plate, both ends of the stabilizing clamping plate are arranged in the limiting clamping grooves, and the limiting clamping guide rod passes through the end of the stabilizing clamping plate.
[0012] Preferably, a magnetic frame cover is installed on the top of the culture box, an embedding groove is opened at the top edge of the culture box, the top cover of the culture box is provided with a breathable and moisturizing non-woven fabric, a box top plate is installed on the top of the culture main cabinet, a culture placement box is installed on the top of the front surface of the culture main cabinet, a negative pressure suction hole is provided on the rear surface of the culture placement box, a non-woven fabric winding seat is installed on one side of the culture placement box, and a breathable and moisturizing non-woven fabric is rolled up on the non-woven fabric winding seat, a heat dissipation through-hole is provided at the bottom of the front surface of the culture main cabinet, and an operation panel is installed on one side of the heat dissipation through-hole.
[0013] Preferably, the breathable and moisturizing non-woven fabric on the non-woven fabric winding seat is covered on the top of the culture box after cutting, and the cut breathable and moisturizing non-woven fabric is covered on the top of the culture box through a magnetic frame cover and an embedded groove, and a moisturizing water pump is provided at the corner of the culture main body groove, and an atomizing nozzle is installed at one end of the moisturizing water pump to spray water toward the breathable and moisturizing non-woven fabric.
[0014] A method for culturing ganoderma lucidum strains comprises the following steps:
[0015] S1. Material preparation: prepare substrate materials suitable for Ganoderma lucidum cultivation, such as sawdust, straw, and Ganoderma lucidum strains, and process the substrate. Then process the substrate materials and sterilize them by boiling or high temperature to kill the bacteria and microorganisms therein;
[0016] S2, inoculation: evenly spread the Ganoderma lucidum strains on the treated substrate material, and mix the strains in warm water when spreading, and then evenly spray the mixture on the substrate;
[0017] S3, culture: pour the inoculated matrix into the culture box, and then place it in the culture main body tank on the inner side of the workbench for culture, pour a certain amount of water into the culture main body tank before placing, and heat it to a constant temperature through a water bath electric heating wire, and then place the culture box on a box body lifting placement plate that can be lifted and adjusted by a lifting synchronous motor, and clamp it through a stable clamping plate, and after placing it, stir the water flow through a water bath heating stirring plate, and at the same time cut a certain length of breathable and moisturizing non-woven fabric, and the cut breathable and moisturizing non-woven fabric cover is set on the top of the culture box, and is pressed by a magnetic suction frame cover, and during the water bath heating process, the moisturizing water pump works to draw out water, and sprays it through an atomizing nozzle on the surface of the breathable and moisturizing non-woven fabric that is soaked and covered;
[0018] S4. Management and maintenance: Regularly observe and manage factors such as the humidity, temperature and ventilation of the substrate, and observe the growth of the fungus. Remove the infected fungus and retain the uninfected products. When the cap expands and the stipe becomes hard, it can be harvested.
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] (1) When the device is used to cultivate Ganoderma lucidum, the culture box is directly placed in the culture main tank through the culture main tank during cultivation, and the constant temperature is maintained by a water bath. Compared with the air conditioning temperature control system, the temperature inside the culture medium placed in the culture box can be ensured to avoid a certain temperature difference between the inside and the outside, so as to better play a temperature control role and improve the survival rate of Ganoderma lucidum. In addition, the water bath constant temperature can be kept uniform by stirring during use, so as to avoid different water temperatures in the culture main tank during the heating process, which affects the growth environment of Ganoderma lucidum, thereby improving the survival rate of Ganoderma lucidum and better cultivating Ganoderma lucidum for use;
[0021] (2) At the same time, a stable clamping plate is also provided in the culture main body tank. When the culture box is placed in the culture main body tank for constant temperature heating in a water bath, the culture box placed in the culture main body tank is easily shaken due to the stirring of the water flow. Through the function of the stable clamping plate, after the culture box is placed, the culture box can be clamped and stabilized in the culture main body tank by the stable clamping plate, so as to avoid the culture box shaking unsteadily during the water temperature stirring and heating process, thereby affecting the survival of the Ganoderma lucidum strains. At the same time, when the culture box is placed, the lifting and lowering of the box body lifting and placing plate can be adjusted to adapt to the cultivation and placement of culture boxes at different heights, so as to improve the cultivation and use while improving the practicality and stability of the device;
[0022] (3) At the same time, during the cultivation process, it is usually necessary to place the culture box for a certain number of days. After the culture box is placed, the breathable and moisturizing non-woven fabric rolled up on the non-woven fabric rolling seat can be cut into a certain length and covered on the culture box. Then, the magnetic frame cover is embedded in the embedded groove on the top of the culture box to ensure that the breathable and moisturizing non-woven fabric is stably covered on the top of the culture box. At the same time, it is also convenient to disassemble and replace the breathable and moisturizing non-woven fabric. The breathable and moisturizing non-woven fabric is disposable after cutting. After the breathable and moisturizing non-woven fabric is covered, water mist can be sprayed regularly and quantitatively through the atomizing nozzle. The water mist falls on the breathable and moisturizing non-woven fabric to ensure the humidity of the matrix in the culture box and avoid water mist being directly sprayed on the matrix to affect the cultivation effect of Ganoderma lucidum. In addition, the humidity of the matrix in the culture box is ensured by wetting the breathable and moisturizing non-woven fabric, which can better cultivate Ganoderma lucidum, thereby improving the practicality of the device. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a structural schematic diagram of the present invention;
[0024] Figure 2 This is a schematic diagram of the structure of placing a workbench plate of the present invention;
[0025] Figure 3 It is a schematic diagram of the cross-sectional structure of the main culture tank of the present invention;
[0026] Figure 4It is a schematic diagram of the cross-sectional installation structure of the rubber clamping gasket of the present invention;
[0027] Figure 5 It is a schematic diagram of the structure of the culture box of the present invention;
[0028] In the figure: 100, culture cabinet; 101, top plate of the box; 102, culture box; 103, negative pressure suction hole; 104, operation panel; 105, heat dissipation hole; 200, workbench; 201, culture box; 202, culture tank; 203, box lifting plate; 204, liquid hole; 205, lifting screw; 206, lifting limit seat; 207, lifting synchronous motor; 208, water bath heating ring ; 209, water bath electric heating wire; 300, stable clamping plate; 301, rubber clamping gasket; 302, limit clamping guide rod; 303, limit clamping groove; 304, clamping spring; 400, water bath heating stirring plate; 401, stirring protrusion; 402, stirring motor; 500, embedded groove; 501, magnetic frame cover; 502, breathable and moisturizing non-woven fabric; 503, non-woven fabric winding seat; 504, moisturizing water pump; 505, atomizing nozzle. DETAILED DESCRIPTION
[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example 1
[0030] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5The present invention provides a technical solution: biological safety cabinets are widely used in the fields of medical health, disease prevention and control, food hygiene, biopharmaceuticals, environmental monitoring and various biological laboratories. They are an important basis for ensuring biological safety and environmental safety. They are used to prevent aerosol dissipation of certain dangerous or unknown biological particles during experimental operation. The working principle is to use negative pressure filtration technology to shield the aerosols generated when operating infectious samples in a confined space, thereby protecting operators, samples and the environment. A Ganoderma lucidum strain culture screening device includes a culture main cabinet 100, a front surface of the culture main cabinet 100 is fixedly installed with a workbench 200, and the inner side of the workbench 200 is installed There is a culture main body groove 202, in which a culture box 201 is placed, and an adjustable box body lifting and placing plate 203 is arranged on the inner side of the culture main body groove 202, and the culture box 201 is placed on the adjustable box body lifting and placing plate 203, and a stable clamping plate 300 for clamping the culture box 201 is arranged on the inner top of the culture main body groove 202, and a rubber clamping gasket 301 is adhered to one side of the stable clamping plate 300, and the rubber clamping gasket 301 can be used for clamping more stably, and both ends of the stable clamping plate 300 are arranged in the limiting clamping groove 303, and the limiting clamping guide rod 302 passes through the end of the stable clamping plate 300, thereby playing a role of stabilizing and limiting the stable clamping plate 300.
[0031] Specifically, a water bath heating stirring plate 400 for rotating and stirring the water flow is also installed on the inner side of the culture main body tank 202, and a water bath heating ring 208 for heating is arranged at the edge of the water bath heating stirring plate 400 located in the culture main body tank 202, and a lifting synchronous motor 207 is symmetrically fixedly installed at the bottom of the workbench 200, and a rotatable lifting screw 205 is installed on the top of the lifting synchronous motor 207 located in the culture main body tank 202, and an integrated lifting limit seat 206 is arranged on one side of the box body lifting placement plate 203. When the cultivation device in the application is used to cultivate Ganoderma lucidum strains, the prepared Ganoderma lucidum substrate is placed in the culture box 201, and a certain amount of water is poured into the culture main body tank 202 before being placed, and then heated to a constant temperature and then placed. Insulation is carried out. When the culture box 201 is placed inside the workbench 200, the water bath electric heating wire 209 located in the water bath heating ring 208 at the bottom of the culture main groove 202 converts electrical energy into thermal energy, thereby heating the water in the culture main groove 202 to a suitable temperature. When the culture box 201 is placed inside the culture main groove 202, the lifting and lowering of the box body lifting and placing plate 203 can also be adjusted according to the height of the culture box 201. When the box body lifting and placing plate 203 is adjusted, the lifting synchronous motor 207 works to drive the lifting screw 205 to rotate. When the lifting screw 205 rotates, the lifting and lowering of the lifting limit seat 206 is controlled by the thread, thereby driving the box body lifting and placing plate 203 to rise and fall to a suitable height for placing culture boxes 201 of different heights for water bath heating.
[0032] Specifically, the lifting screw 205 passes through the lifting limit seat 206. When the lifting screw 205 rotates, the lifting limit seat 206 can be driven to rise and fall through the thread, and a lifting limit groove is provided at the edge of the culture main body groove 202. The box body lifting and placing plate 203 is limited and lifted in the lifting and placing groove by the lifting limit seat 206 and the lifting screw 205, ensuring that the box body lifting and placing plate 203 is stably lifted and lowered. At the same time, two groups of box body lifting and placing plates 203 are symmetrically arranged at both ends of the inner side of the culture main body groove 202, and the surface of the box body lifting and placing plate 203 is provided with a through liquid through hole 204, which is better Water bath heating is used. Specifically, the bottom of the workbench 200 is placed directly below the culture main tank 202 and a stirring motor 402 is fixedly installed thereon. The top of the stirring motor 402 is located in the culture main tank 202 and a water bath heating stirring plate 400 is installed thereon. The water bath heating stirring plate 400 is driven to rotate by the stirring motor 402. An integrated stirring protrusion 401 is provided on the water bath heating stirring plate 400. A water bath electric heating wire 209 is embedded on the inner side of the water bath heating ring 208. Both the water bath heating ring 208 and the water bath electric heating wire 209 are annular structures for better heating.
[0033] Specifically, a limited clamping groove 303 is provided on the inner top of the culture main groove 202, and a limited clamping guide rod 302 is fixedly installed on the inner side of the limited clamping groove 303, and a clamping spring 304 is sleeved on the limited clamping guide rod 302. A stabilizing clamping plate 300 is also installed on the inner side of the culture main groove 202. When the height adjustment of the box body lifting and placing plate 203 is completed, the culture box 201 containing the Ganoderma lucidum cultivation matrix is placed on the box body lifting and placing plate 203 at both ends of the inner side of the culture main groove 202. At the same time, the bottom of the culture box 201 is in contact with the water heated by constant temperature, and the temperature of the matrix in the culture box 201 is maintained by the water bath. At the same time, during the water bath heating process, in order to prevent the water temperature of the upper layer and the lower layer from being different, the stirring motor 402 can work synchronously during the water bath heating, driving the water bath heating stirring plate 400 to rotate, and the stirring protrusion 401 on the water bath heating stirring plate 400 can be stirred by stirring. The water flow is stirred to ensure constant temperature heating of the water bath, avoid different temperatures between the upper layer and the lower layer during water bath heating, better ensure the temperature of the culture substrate during Ganoderma lucidum cultivation, and improve the survival rate of Ganoderma lucidum. At the same time, during the water bath heating process, the stirring water flow will drive the culture box 201 placed on the box body lifting and placing plate 203 to shake. When the culture box 201 is placed in the culture main body groove 202, the stabilizing clamping plate 300 can be pushed to both sides of the limiting clamping groove 303 before placement. The stabilizing clamping plate 300 moves to the upper limit of the limiting clamping guide rod 302 on the inner side of the limiting clamping groove 303, and squeezes the clamping spring 304 to deform. When the culture box 201 is placed, the rebound force generated by the deformation of the clamping spring 304 pushes the stabilizing clamping plate 300, so that the placed culture box 201 is clamped stably by the stabilizing clamping plate 300, avoiding the culture box 201 from shaking unsteadily during the stirring process. Example 2
[0034] See also Figure 1 , Figure 3 and Figure 5The present invention provides a technical solution: a ganoderma lucidum strain culture and screening device, a magnetic frame cover 501 is installed on the top of the culture box 201, an embedding groove 500 is opened at the top edge of the culture box 201, a breathable and moisturizing non-woven fabric 502 is provided on the top cover of the culture box 201, and the breathable and moisturizing non-woven fabric 502 covered is cut from a non-woven fabric winding seat 503, a box top plate 101 is fixedly installed on the top of the culture main cabinet 100, a culture placement box 102 is installed on the top of the front surface of the culture main cabinet 100, a negative pressure suction hole 103 is provided on the rear surface of the culture placement box 102, a non-woven fabric winding seat 503 is fixedly installed on one side of the culture placement box 102, and the non-woven fabric A breathable and moisturizing non-woven fabric 502 is rolled up on the roll-up seat 503, a heat dissipation through hole 105 is provided at the bottom of the front surface of the culture main body cabinet 100, an operation panel 104 is installed on one side of the heat dissipation through hole 105, the breathable and moisturizing non-woven fabric 502 on the non-woven fabric roll-up seat 503 is covered on the top of the culture box 201 after cutting, the cut breathable and moisturizing non-woven fabric 502 is covered on the top of the culture box 201 through the magnetic suction frame cover 501 and the embedded groove 500, and a moisturizing water pump 504 is provided at the corner of the culture main body groove 202, and an atomizing nozzle 505 for spraying water toward the breathable and moisturizing non-woven fabric 502 is installed at one end of the moisturizing water pump 504. When the culture box 201 is placed on the culture main body, the moisture-absorbing non-woven fabric 502 is sprayed with water. After the inside of the groove 202 is formed, the breathable and moisturizing non-woven fabric 502 located on the non-woven fabric winding seat 503 on the inner wall of the culture placement box 102 can be cut into a suitable length and then laid on the top of the culture box 201. Then, the magnetic frame cover 501 is aligned with the embedded groove 500 on the top of the culture box 201 and adsorbed. The breathable and moisturizing non-woven fabric 502 is clamped and covered on the top of the culture box 201 through the magnetic frame cover 501 to better protect the culture matrix in the culture box 201 and reduce the possibility of infection. At the same time, the breathable and moisturizing non-woven fabric 502 is a disposable product, and the adsorption cover is convenient for disassembly and replacement, which is convenient for cultivation and use. During the cultivation process, it is placed in the culture main groove 20 The culture box 201 inside is covered with a breathable and moisturizing non-woven fabric 502 at the top. During the culture placement process, the atomizing nozzle 505 can be rotated to the top of the culture box 201, and then the water flow in the culture main tank 202 is extracted through the moisturizing water pump 504, and discharged through the atomizing nozzle 505 to be sprayed on the breathable and moisturizing non-woven fabric 502 covering the top of the culture box 201, so as to always keep the breathable and moisturizing non-woven fabric 502 moist. The atomizing nozzle 505 can spray water mist regularly and quantitatively, so as to ensure that the matrix inside the culture box 201 is always moist, avoid spraying directly on the matrix, which affects the activity of the Ganoderma lucidum strains, and better cultivate and screen out good products, thereby improving the practicality of the device. Example 3
[0035] Referring to Example 1 and Example 2 and in combination with the accompanying drawings, the present invention provides a technical solution: a method for culturing Ganoderma lucidum, comprising the following steps;
[0036] S1. Material preparation: prepare substrate materials suitable for Ganoderma lucidum cultivation, such as sawdust, straw, and Ganoderma lucidum strains, and process the substrate. Then process the substrate materials and sterilize them by boiling or high temperature to kill the bacteria and microorganisms therein;
[0037] S2, inoculation: evenly spread the Ganoderma lucidum strains on the treated substrate material, and mix the strains in warm water when spreading, and then evenly spray the mixture on the substrate;
[0038] S3, culture: pour the inoculated matrix into the culture box 201, and then place it in the culture main body tank 202 on the inner side of the workbench 200 for culture, pour a certain amount of water into the culture main body tank 202 before placing it, and heat it to a constant temperature through the water bath electric heating wire 209, then place the culture box 201 on the box body lifting placement plate 203 that can be lifted and adjusted by the lifting synchronous motor 207, and clamp it through the stable clamping plate 300, after placing it, stir the water flow through the water bath heating stirring plate 400, and at the same time cut a certain length of breathable and moisturizing non-woven fabric 502, the cut breathable and moisturizing non-woven fabric 502 is covered on the top of the culture box 201, and is pressed by the magnetic suction frame cover 501, and during the water bath heating process, the moisturizing water pump 504 works to extract water flow, and sprays it through the atomizing nozzle 505 on the surface of the breathable and moisturizing non-woven fabric 502 that is soaked and covered;
[0039] S4. Management and maintenance: Regularly observe and manage factors such as the humidity, temperature and ventilation of the substrate, and observe the growth of the fungus. Remove the infected fungus and retain the uninfected products. When the cap expands and the stipe becomes hard, it can be harvested.
[0040] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A Ganoderma lucidum strain culture and screening device, comprising a culture main cabinet (100), characterized in that: The front surface of the culture main body cabinet (100) is provided with a placing workbench (200), the inner side of the placing workbench (200) is provided with a culture main body tank (202), a culture box (201) is placed in the culture main body tank (202), a box body lifting and placing plate (203) that can be raised and lowered is provided on the inner side of the culture main body tank (202), the culture box (201) is placed on the box body lifting and placing plate (203) that can be raised and lowered, a stable clamping plate (300) for clamping the culture box (201) is provided on the inner top of the culture main body tank (202), and a water bath heating stirring plate (400) for rotating and stirring water flow is also provided on the inner side of the culture main body tank (202), and a water bath heating ring (208) for heating is provided at the edge of the water bath heating stirring plate (400) located in the culture main body tank (202); A lifting synchronous motor (207) is symmetrically installed at the bottom of the placement workbench (200), a lifting screw (205) is installed at the top of the lifting synchronous motor (207) located in the culture main body groove (202), and a lifting limit seat (206) is provided on one side of the box body lifting placement plate (203); The bottom of the workbench (200) is located directly below the culture main body tank (202) and is equipped with a stirring motor (402); the top of the stirring motor (402) is located in the culture main body tank (202) and is equipped with a water bath heating stirring plate (400); and the water bath heating stirring plate (400) is provided with a stirring protrusion (401); A limited clamping groove (303) is provided at the inner top of the culture main body groove (202), a limited clamping guide rod (302) is installed inside the limited clamping groove (303), a clamping spring (304) is installed on the limited clamping guide rod (302), and a stabilizing clamping plate (300) is also installed inside the culture main body groove (202); A magnetic frame cover (501) is installed on the top of the culture box (201); a groove (500) is provided at the top edge of the culture box (201); a breathable and moisturizing non-woven fabric (502) is provided on the top cover of the culture box (201); a box top plate (101) is installed on the top of the culture main cabinet (100); a culture placement box (102) is installed on the top of the front surface of the culture main cabinet (100); a negative pressure suction hole (103) is provided on the rear surface of the culture placement box (102); a non-woven fabric rolling seat (503) is installed on one side of the culture placement box (102); a breathable and moisturizing non-woven fabric (502) is rolled on the non-woven fabric rolling seat (503); a heat dissipation through hole (105) is provided at the bottom of the front surface of the culture main cabinet (100); and an operation panel (104) is installed on one side of the heat dissipation through hole (105); The breathable and moisturizing non-woven fabric (502) on the non-woven fabric winding seat (503) is cut and covered on the top of the culture box (201); the cut breathable and moisturizing non-woven fabric (502) is covered on the top of the culture box (201) through the magnetic frame cover (501) and the embedded groove (500); a moisturizing water pump (504) is provided at the corner of the culture main groove (202); and an atomizing nozzle (505) for spraying water toward the breathable and moisturizing non-woven fabric (502) is installed at one end of the moisturizing water pump (504).
2. A Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: The lifting screw (205) passes through the lifting limit seat (206), and a lifting limit slot is provided at the edge of the culture main body groove (202). The box body lifting and placing plate (203) is limited and lifted in the lifting and placing limit slot through the lifting limit seat (206) and the lifting screw (205). At the same time, two groups of box body lifting and placing plates (203) are symmetrically arranged at both ends of the inner side of the culture main body groove (202), and the surface of the box body lifting and placing plate (203) is provided with a through liquid through hole (204).
3. The device for cultivating and screening Ganoderma lucidum strains according to claim 1, characterized in that: A water bath electric heating wire (209) is arranged on the inner side of the water bath heating ring (208), and both the water bath heating ring (208) and the water bath electric heating wire (209) are annular structures.
4. The device for cultivating and screening Ganoderma lucidum strains according to claim 1, characterized in that: A rubber clamping gasket (301) is provided on one side of the stabilizing clamping plate (300), both ends of the stabilizing clamping plate (300) are arranged in the limiting clamping grooves (303), and the limiting clamping guide rod (302) passes through the ends of the stabilizing clamping plate (300).
5. The method for cultivating and screening a Ganoderma lucidum strain according to any one of claims 1 to 4, characterized in that: The steps include: S1. Material preparation: prepare substrate materials suitable for Ganoderma lucidum cultivation, prepare Ganoderma lucidum strains, process the substrate, and then process the substrate materials and sterilize them at high temperature to kill the microorganisms therein; S2, inoculation: evenly spread the Ganoderma lucidum strains on the treated substrate material, and mix the strains in warm water when spreading, and then evenly spray the mixture on the substrate; S3, culture: pour the inoculated matrix into the culture box (201), and then place it in the culture main body groove (202) on the inner side of the workbench (200) for culture. Pour a certain amount of water into the culture main body groove (202) and heat it to a constant temperature through the water bath electric heating wire (209). Then place the culture box (201) on the box body lifting and placing plate (203) that can be raised and lowered by the lifting synchronous motor (207), and stabilize the clamping plate (203) to adjust the lifting and lowering of the box body. 300) is clamped, and after being placed, the water flow is stirred by a water bath heating stirring plate (400), and at the same time, a certain length of breathable and moisturizing non-woven fabric (502) is cut, and the cut breathable and moisturizing non-woven fabric (502) is covered on the top of the culture box (201) and pressed by a magnetic suction frame cover (501). During the water bath heating process, the moisturizing water pump (504) works to extract water flow, and sprays it on the surface of the breathable and moisturizing non-woven fabric (502) that is soaked, cut and covered; S4. Management and maintenance: Regularly observe and manage the humidity, temperature and ventilation factors of the substrate, and observe the growth of the fungus. Remove the infected fungus and retain the uninfected products. When the cap expands and the stipe becomes hard, it can be harvested.
Citation Information
Patent Citations
Biological safety cabinet for ganoderma lucidum strain culture
CN220926752U
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