A simulated remediation system based on plant-enhanced microbial remediation

Abstract

The present invention discloses a simulation remediation system based on plant-enhanced microbial remediation, which relates to the technical field of soil pollution prevention and control, and includes a first sand box; the surface soil of the soil layer contained in the first sand box contains composite pollutants including organic pollutants and heavy metal pollutants; the first sand box is provided with a water inlet group and a water outlet group; a baffle is fixedly provided in the first sand box, and the baffle separates the surface soil of the soil layer into a plant microbial agent area and a plant area; a remediation microbial agent is added to the plant microbial agent area, and the plant microbial agent area and the plant area are both planted with the same hyperaccumulator plants; the plant microbial agent area and the plant area are both provided with the same monitoring well group, injection well group and extraction well group. It is used to explore the migration laws of various pollutants in soil and groundwater, test the remediation effect of plant-enhanced microorganisms, and realize real-time monitoring of various physical and chemical parameters in the soil remediation process.

Description

Technical Field

[0001] The present invention relates to the technical field of soil pollution prevention and control, and in particular to a simulated remediation system based on plant-enhanced microbial remediation. Background Art

[0002] The soil of the compound pollution sites left by coking plants, solvent plants, electroplating plants, chromium salt plants and other industrial enterprises mostly exists in the form of heavy metal (such as arsenic, chromium, cadmium) compound pollution, organic compound pollution (such as polycyclic aromatic hydrocarbons, chlorinated hydrocarbons, etc.), heavy metal and organic compound pollution. The compound pollutants in the soil of the sites have become "chemical time bombs" around people, seriously threatening the regional ecological environment safety and human health.

[0003] Heavy metal hyperaccumulators can effectively absorb heavy metals in the surface soil, and can recruit and assemble specific rhizosphere functional microbial populations in their roots, which can coordinate plant remediation by affecting the morphology and fate of heavy metals in the medium. At present, most studies focus on the interaction between pollutants and microorganisms in a single medium (such as surface soil or aquifer). However, in actual contaminated sites, pollutants will infiltrate from the soil surface through the aeration zone and aquifer. Therefore, investigating the differences in soil types, hydrogeology and environmental factors (such as pH, redox zones), and the effects on the spatial distribution characteristics (horizontal and vertical) of pollutants and the spatiotemporal succession of functional microbial communities will be a new trend in studying the in situ remediation of site pollutants by microorganisms. In addition, the use of indigenous or exogenous rhizosphere microorganisms is an important means of in situ enhanced plant remediation. At present, some preliminary studies have been conducted on the response and transformation mechanism of indigenous rhizosphere microorganisms to single pollutants, but research on the interaction between plants, microorganisms and multiple metals and the coupled remediation mechanism for complex contaminated sites has not yet been carried out. In recent years, the emerging synthetic biology has taken the metabolic synergy and functional complementarity of microbial communities as the starting point, providing the possibility for designing and constructing complex microbial communities with targeted remediation functions, which have stronger environmental adaptability and stability than the single strain enhancement method. Basic research on how synthetic microbiomes can collaborate with plants to remove pollutants through communication networks is in the ascendant, becoming the frontier and hot spot of in situ bioremediation research on complex contaminated sites. Summary of the invention

[0004] The purpose of the present invention is to provide a simulated remediation system based on plant-enhanced microbial remediation to solve the problems existing in the above-mentioned prior art, to explore the migration patterns of various pollutants in soil and groundwater, to test the remediation effect of plant-enhanced microorganisms, and to realize real-time monitoring of various physical and chemical parameters during soil remediation.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a simulated remediation system based on plant-enhanced microbial remediation, comprising a first sand box; a soil layer is contained in the first sand box, and the surface soil of the soil layer contains composite pollutants, and the composite pollutants include organic pollutants and heavy metal pollutants; a water inlet group is arranged on one side of the first sand box, and a water outlet group is arranged on the other side opposite to the first sand box, and the water inlet group is higher than the water outlet group; the water inlet group is connected to a water supply device, and the water outlet group is connected to a wastewater recovery device; the water supplied by the water supply device forms groundwater at the bottom of the first sand box; a baffle is fixedly arranged in the first sand box, The baffle separates the surface soil of the soil layer in the first sand box into a plant inoculant area and a plant area, and the plant inoculant area and the plant area have the same area size; the plant inoculant area is added with repair bacteria, and the plant inoculant area and the plant area are both planted with the same hyperaccumulator plants; the plant inoculant area and the plant area are both provided with the same monitoring well group, injection well group and extraction well group; the monitoring well group is provided with monitoring probes of the soil monitoring system and the groundwater monitoring system; the injection well group is provided with a drug injection hole for communicating with the interior of the first sand box; the extraction well group is provided with an extraction port communicating with the interior of the first sand box.

[0007] Preferably, the plant inoculant area and the plant area both include two planting areas and one non-rhizosphere area, the non-rhizosphere area is located between the two planting areas, and the planting area is used to plant the hyperaccumulator plants; the non-rhizosphere area and the adjacent planting areas are separated by a first gauze; the water inlet group includes two first water inlets; the water outlet group includes two first water outlets; one end of each of the non-rhizosphere areas is connected and communicated with one of the first water inlets, and the other end is connected and communicated with one of the first water outlets.

[0008] Preferably, the monitoring well group and the injection well group both include multiple well groups, and the well groups include deep wells and shallow wells; the upper ends of the deep wells and the shallow wells are both higher than the upper plane of the soil layer in the first sand box; the lower end of the deep well is connected to the groundwater in the first sand box; the lower end of the shallow well is located in the soil layer in the first sand box.

[0009] Preferably, the lower ends of the deep well and the shallow well are provided with a screen tube, the side walls of the screen tube are provided with screen holes, a filter mesh tube is sleeved on the outer side of the screen tube, and gravel is filled between the screen tube and the filter mesh tube.

[0010] Preferably, the hyperaccumulator plants include alfalfa, Leucaena leucophylla and Sedum roxburghii; a row of Leucaena leucophylla is planted in the planting area close to the non-rhizosphere zone, and a row of alfalfa and a row of Sedum roxburghii are planted on the side of Leucaena leucophylla away from the non-rhizosphere zone; the hyperaccumulator plants planted in the two planting areas on both sides of the non-rhizosphere zone are symmetrical to each other.

[0011] Preferably, at least one side wall of the first sand box is made of transparent material.

[0012] Preferably, the top opening of the first sand box is also provided with a heat-insulating cover that can be opened and closed.

[0013] Preferably, a lighting system that can be raised and lowered is provided above the top opening of the first sand box.

[0014] Preferably, the first sand box is fixedly mounted on a stand; the water supply device comprises a water storage bucket and a water inlet flow control valve, the water storage bucket is located higher than the height of the first sand box, the output port of the water storage bucket is connected and communicated with the water inlet group via a connecting pipeline, the water inlet flow control valve is arranged on the connecting pipeline, and the water inlet flow control valve is used to control the flow rate of the fluid in the connecting pipeline.

[0015] Preferably, a second sand box is fixedly arranged on the stand, and the second sand box serves as a blank control group of the first sand box.

[0016] Compared with the prior art, the present invention has achieved the following technical effects:

[0017] The simulated remediation system based on plant-enhanced microbial remediation provided by the present invention sets up a plant microbial agent area, in which hyperaccumulators are planted and remediation agents are added, and uses indigenous microbial communities and added remediation agents (i.e., special functional microbial populations) to construct efficient enhanced microbial flora, and plants hyperaccumulators. Hyperaccumulators can effectively absorb heavy metals in the surface soil, and can recruit and assemble specific rhizosphere functional microbial populations at the roots, thereby improving the transformation effect of rhizosphere microorganisms on heavy metal pollutants and the plant extraction effect, establishing a microbial-plant joint remediation system, and realizing the remediation of complex pollutants; and the surface soil of the soil layer of the first sand box is divided into a plant microbial agent area and a plant area by a baffle, and only hyperaccumulators are planted in the plant area, while in addition to planting the same hyperaccumulators, remediation agents are also added in the plant microbial agent area. The difference between the two areas is whether remediation agents are added, which can be used as a parallel comparison of the effects of remediation agents; and the same plant microbial agent area and plant area are set up. Different monitoring well groups, injection well groups and extraction well groups are used to investigate the valence state transformation process and influencing factors of complex pollutants in soil (horizontal and vertical interfaces) and aquifers under the action of plant-enhanced microbial remediation during the process of plant-enhanced microbial remediation; study the spatial distribution and succession laws of indigenous microbial communities in soil and aquifers of complex polluted sites, analyze the molecular mechanism and feedback regulation mechanism of the biotransformation process of different heavy metals by specific functional microbial populations, clarify the temporal and synergistic interaction mechanism of the transformation of heavy metal complex pollutants by functional microbial communities; investigate the extraction and transformation effects of hyperaccumulators on complex pollutants; study the dynamic changes of the structure of rhizosphere microbial communities and functional genes of heavy metal transformation, and clarify the mechanism of plants and their joint purification of heavy metal soils with microbial groups; explore the migration laws of various pollutants in soil and groundwater, experiment with the remediation effect of plant-enhanced microorganisms, and realize real-time monitoring of various physical and chemical parameters in the remediation process of soil containing complex pollutants.

[0018] Furthermore, both the plant inoculant area and the plant area include two planting areas and one non-rhizosphere area. Whether for the plant inoculant area or the plant area, further horizontal comparison can be carried out to compare the change process of complex pollutants in the soil between the planting area and the non-rhizosphere area to further refine the research.

[0019] Furthermore, both the monitoring well group and the injection well group include a plurality of well groups consisting of deep wells and shallow wells, which can monitor various parameters of soil and groundwater at different locations and add additives.

[0020] Furthermore, the provided screen tubes, gravel materials and filter mesh tubes can effectively reduce soil clogging of the screen holes and ensure good connectivity and monitoring status.

[0021] Furthermore, the hyperaccumulator plants are centered on Leucaena leucaena, with alfalfa and Sedum robur planted on both sides. Since they have different restoration mechanisms and characteristics, when they are planted at the same time in soil containing complex pollutants, they can produce a synergistic effect and jointly improve the restoration efficiency; the high biomass and high enrichment ability of alfalfa and Leucaena leucaena can absorb a large amount of heavy metals and organic pollutants in the soil, while Sedum robur can store heavy metals in the plant body in a non-toxic form through its unique detoxification mechanism, thereby effectively reducing the pollutant content in the soil; the simultaneous planting of the three plants can also promote the growth and activity of soil microorganisms, enhance biodegradation, and further accelerate the degradation and transformation of pollutants in the soil.

[0022] Furthermore, at least one side wall is made of a transparent material, so that the experimenter can observe the internal conditions of the first sand box more intuitively.

[0023] Furthermore, the setting of the insulation cover can be closed when the ambient temperature is too low or too high, providing a good temperature environment inside the first sand box, ensuring a healthy growth environment for the hyperaccumulator plants in the first sand box, and ensuring effective control of water evaporation in the first sand box.

[0024] Furthermore, the lighting system can provide a sunlight-simulating environment in each sand box, and simulate different sunlight effects during the day by lifting and lowering.

[0025] Furthermore, the water supply device adopts a water storage bucket, and controls the water inlet flow rate through a water inlet flow control valve. Its structure is simple, which facilitates the experimenter to accurately control the experimental conditions.

[0026] Furthermore, the second sand box is set up as a blank control group of the first sand box, which can form a control for the plant fungus agent area and the plant area. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 This is a schematic diagram of the overall structure of the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0029] Figure 2 A side view of a simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0030] Figure 3A top view of a simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0031] Figure 4 A schematic diagram of a plant inoculant area and a plant area in a first sand box in a simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0032] Figure 5 A schematic diagram of planting hyperaccumulators in the plant microbial agent area or plant area in the first sand box in the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0033] Figure 6 A schematic diagram of the arrangement of the monitoring well group, the injection well group and the extraction well group in the first sand box in the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0034] Figure 7 A schematic diagram of the arrangement depths of deep wells and shallow wells in the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0035] Figure 8 The overall diagram of the control system in the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention;

[0036] Fig. 9 This is a schematic diagram of the control of the display device in the simulated remediation system based on plant-enhanced microbial remediation provided by the present invention.

[0037] In the figure:

[0038] 100-Simulated remediation system based on plant-enhanced microbial remediation;

[0039] 10-first sand box; 11-baffle; 12-plant inoculant area; 121-planting area; 122-non-rhizosphere area; 123-first gauze; 124-second gauze; 13-plant area; 14-monitoring well group; 15-injection well group; 16-extraction well group; 17-screen tube; 171-filter mesh tube; 172-gravel; 18-hyperaccumulator plant;

[0040] 20- second sand box;

[0041] 30-stand; 31-insulation cover; 32-lighting system; 33-sprinkler; 34-platform; 35-guardrail; 36-stairs;

[0042] 40-water storage bucket; 41-water inlet flow control valve;

[0043] 50-wastewater collection pretreatment barrel; 51-wastewater treatment system; 52-water outlet flow control valve; 53-wastewater treatment pump flow reuse system;

[0044] 60-PLC controller. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] The purpose of the present invention is to provide a simulation remediation system based on plant-enhanced microbial remediation to solve the problems existing in the prior art, to explore the migration patterns of various pollutants in soil and groundwater, to test the remediation effects of plant-enhanced microorganisms, and to realize real-time monitoring of various physical and chemical parameters during soil remediation.

[0047] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0048] Embodiment 1

[0049] This embodiment provides a simulated remediation system 100 based on plant-enhanced microbial remediation, such as Figures 1 to 9 As shown, it includes a first sand box 10; a soil layer is contained in the first sand box 10, and the surface soil of the soil layer contains complex pollutants, and the complex pollutants include organic pollutants and heavy metal pollutants; a water inlet group is arranged on one side of the first sand box 10, and a water outlet group is arranged on the other side opposite to it, and the water inlet group is higher than the water outlet group; the water inlet group is connected to a water supply device, and the water outlet group is connected to a wastewater recovery device; the water supplied by the water supply device forms groundwater at the bottom of the first sand box 10; a baffle 11 is fixedly arranged in the first sand box 10, and the baffle 11 separates the surface soil of the soil layer in the first sand box 10 into The plant inoculant area 12 and the plant area 13 have the same area size; the plant inoculant area 12 is added with restoration bacteria, and the plant inoculant area 12 and the plant area 13 are both planted with the same hyperaccumulator 18; the plant inoculant area 12 and the plant area 13 are both provided with the same monitoring well group 14, injection well group 15 and extraction well group 16; the monitoring well group 14 is provided with monitoring probes of the soil monitoring system and the groundwater monitoring system; the injection well group 15 is provided with an agent injection hole for communicating with the interior of the first sand box 10; the extraction well group 16 is provided with an extraction port communicating with the interior of the first sand box 10.

[0050] By setting up a plant microbial agent area 12, in which hyperaccumulators 18 are planted and remediation agents are added, the indigenous microbial community and the added remediation agents (i.e., special functional microbial populations) are used to construct an efficient and enhanced microbial community, and hyperaccumulators 18 are planted. The hyperaccumulators 18 can effectively absorb heavy metals in the surface soil, and can recruit and assemble specific rhizosphere functional microbial populations at the roots, thereby improving the transformation effect and plant extraction effect of rhizosphere microorganisms on heavy metal pollutants, establishing a microbial-plant joint remediation system, and realizing the remediation of complex pollutants; and the surface soil of the soil layer of the first sand box 10 is divided into a plant microbial agent area 12 and a plant area 13 by a baffle 11, in which only hyperaccumulators 18 are planted, and in addition to the same hyperaccumulators 18, remediation agents are added to the plant microbial agent area 12. The difference between the two areas is whether remediation agents are added, which can be used as a parallel comparison of the effects of remediation agents; the same monitoring well group 14 and injection well group 13 are set in both the plant microbial agent area 12 and the plant area 13. Group 15 and extraction well group 16, so as to realize the investigation of the valence state transformation process and influencing factors of complex pollutants in soil (horizontal and vertical interfaces) and aquifers under the action of plant-enhanced microbial remediation during the process of plant-enhanced microbial remediation; study the spatial distribution and succession law of indigenous microbial communities in soil and aquifers of complex polluted sites, analyze the molecular mechanism and feedback regulation mechanism of the biotransformation process of different heavy metals by specific functional microbial populations, clarify the temporal and synergistic interaction mechanism of the functional microbial community on the transformation of heavy metal complex pollutants; investigate the extraction and transformation effect of hyperaccumulator plant 18 on complex pollutants; study the dynamic changes of the community structure and functional genes of rhizosphere microorganisms for heavy metal transformation, and clarify the mechanism of plants and their joint purification of heavy metal soil with microbial groups; explore the migration law of various pollutants in soil and groundwater, experiment the remediation effect of plant-enhanced microorganisms, realize the real-time monitoring of various physical and chemical parameters in the remediation process of soil containing complex pollutants, and fill the gap in the pilot test equipment of domestic soil remediation technology.

[0051] The composition of the simulated remediation system 100 based on plant-enhanced microbial remediation in this embodiment is as follows:

[0052] Specifically, it includes a stand 30, a first sand box 10, a second sand box 20, a water supply device, a water spray system, a wastewater recovery device, a heat preservation cover 31, a lighting system 32, a controller, a soil monitoring system and a groundwater monitoring system.

[0053] Among them, the relevant settings of the stand 30 are:

[0054] Specifically, the stand 30 is fixed on the ground.

[0055] Specifically, the first flask 10 and the second flask 20 have the same size, and the first flask 10 and the second flask 20 are located at the same height on the stand 30 .

[0056] Specifically, the stand 30 is provided with a platform 34 on the top of the first flask 10 and the second flask 20 , a guardrail 35 is provided around the platform 34 , and a staircase 36 is provided between the platform 34 and the ground, and a handrail is provided on the side of the staircase 36 .

[0057] Among them, the related settings of the first sand box 10 and the second sand box 20 are as follows:

[0058] Specifically, the size of the first flask 10 and the second flask 20 are both 2m*2m*2m.

[0059] Specifically, regarding the relevant settings in the first sand box 10:

[0060] Specifically, a synthetic remediation bacterial community with nitrogen-fixing microorganisms as the core was constructed, the combined remediation effects of multiple functional remediation plants and synthetic bacterial communities were evaluated, and the combined remediation mechanism of synthetic microbiome and functional plants based on nitrogen-fixing function was explored.

[0061] Specifically, the soil layers in the first sand box 10 are specifically arranged as follows: the top 40 cm layer is silt, which is the topsoil; below the silt is an 80 cm deep soil layer of silt and fine sand in a 1:1 ratio; and further down is an 80 cm deep layer of fine sand.

[0062] Specifically, the baffle 11 separates the topsoil in the first sand box 10 into two identical halves, and one of the halves is taken as an example for explanation: its width is 1m, and in its width direction, a first gauze 123 is set 40cm away from both ends, and the distance between the two first gauze nets 123 is 20cm. The non-root zone 122 is between the two first gauze nets 123, and the bottom of the first gauze net 123 is flush with the bottom of the first sand box 10, and the two ends of the first gauze net 123 are flush with the corresponding side walls of the first sand box 10 respectively.

[0063] Specifically, the upper end of the baffle 11 is 0.5 m higher than the surface soil to avoid contamination of the plant area 13 when spraying the restoration bacteria agent.

[0064] Among the optional solutions of this embodiment, it is more preferred that Figure 1 to Figure 3 and Figure 5As shown, the plant microbial agent area 12 and the plant area 13 both include two planting areas 121 and a non-rhizosphere area 122, the non-rhizosphere area 122 is located between the two planting areas 121, and the planting area 121 is used to plant hyperaccumulators 18; the non-rhizosphere area 122 and the adjacent planting area 121 are separated by a first gauze 123; the water inlet group includes two first water inlets; the water outlet group includes two first water outlets; one end of each non-rhizosphere area 122 is connected and communicated with a first water inlet, and the other end is connected and communicated with a first water outlet. The plant microbial agent area 12 and the plant area 13 both include two planting areas 121 and a non-rhizosphere area 122. Whether for the plant microbial agent area 12 or the plant area 13, further horizontal comparison can be performed to compare the change process of complex pollutants in the soil between the planting area 121 and the non-rhizosphere area 122, and further refine the research.

[0065] Specifically, the second gauze 124 is provided on the side of the plant inoculant area 12 and the plant area 13 having the first water inlet and the side having the first water outlet.

[0066] Among the optional solutions of this embodiment, it is more preferred that Figure 4 and Figure 5 As shown, the hyperaccumulator plants 18 include alfalfa, Leucaena leucophylla and Sedum roxburghii; a row of Leucaena leucophylla is planted near the non-rhizosphere zone 122 in the planting zone 121, and a row of alfalfa and a row of Sedum roxburghii are planted on the side of Leucaena leucophylla away from the non-rhizosphere zone 122; the hyperaccumulator plants 18 planted in the two planting zones 121 on both sides of the non-rhizosphere zone 122 are symmetrical to each other. Hyperaccumulator plant 18 uses Leucaena leucaena as the center, with alfalfa and Sedum robur planted on both sides. Since each has different restoration mechanisms and characteristics, when planted at the same time in soil containing complex pollutants, they can produce a synergistic effect and jointly improve the restoration efficiency; the high biomass and high enrichment ability of alfalfa and Leucaena leucaena can absorb a large amount of heavy metals and organic pollutants in the soil, while Sedum robur can store heavy metals in the plant body in a non-toxic form through its unique detoxification mechanism, thereby effectively reducing the pollutant content in the soil; the simultaneous planting of the three plants can also promote the growth and activity of soil microorganisms, enhance biodegradation, and further accelerate the degradation and transformation of pollutants in the soil.

[0067] Specifically, the arrangement of alfalfa, leucaena leucaena and sedum is as follows: Figure 5 shown.

[0068] In the optional solution of this embodiment, it is more preferred that at least one side wall of the first sand box 10 is made of transparent material. At least one side wall is made of transparent material, so that the experimenter can observe the internal situation of the first sand box 10 more intuitively.

[0069] Specifically, organic pollutants: PCB52 (2 mg / kg), B[a]P (2 mg / kg), sulfamethoxazole (10 mg / kg), TCE (20 mg / kg), PFOS (0.01 mg / kg); heavy metal pollutants: Cd (5 mg / kg), Cr (50 mg / kg), As (50 mg / kg).

[0070] Specifically, the monitoring well group 14, the injection well group 15 and the extraction well group 16 are arranged in the first sand box 10 as follows: Figure 6 shown.

[0071] Specifically, the positions of the first water inlet and the first water outlet are explained by taking the first sand box 10 as an example; the height of the first water inlet is 120 cm from the top, and the height of the first water outlet is 200 cm from the top. The specific water level adjustment depends on the grid effect of each mesh in the first sand box 10 and the external pipeline regulation.

[0072] Specifically, regarding the relevant settings in the second sand box 20:

[0073] Among the optional solutions of this embodiment, it is more preferred that Figure 1 to Figure 3 As shown, a second sand box 20 is fixedly arranged on the stand 30, and the second sand box 20 serves as a blank control group of the first sand box 10. The second sand box 20 is arranged as a blank control group of the first sand box 10, which can form a control for the plant inoculant area 12 and the plant area 13.

[0074] Specifically, at least one side wall of the second flask 20 is made of a transparent material.

[0075] Specifically, the second sand box 20 can be set with the same basic conditions as those in the first sand box 10 according to experimental needs, such as contaminated soil with the same pollutant type and concentration, the same soil temperature and humidity, and groundwater level.

[0076] Among them, the relevant settings of the water supply device:

[0077] Among the optional solutions of this embodiment, it is more preferred that Figure 1 to Figure 3 As shown, the first sand box 10 is fixedly arranged on the stand 30; the water supply device includes a water storage bucket 40 and a water inlet flow control valve 41. The position of the water storage bucket 40 is higher than the height of the first sand box 10. The output port of the water storage bucket 40 is connected and communicated with the water inlet group through a connecting pipeline. The water inlet flow control valve 41 is arranged on the connecting pipeline, and the water inlet flow control valve 41 is used to control the flow size of the fluid in the connecting pipeline. The water supply device adopts a water storage bucket 40 and realizes the control of the water inlet flow through the water inlet flow control valve 41. Its structure is simple, which is convenient for the experimenter to accurately control the experimental conditions.

[0078] Specifically, the water supply device serves as a common water supply facility for the first sand box 10 and the second sand box 20 .

[0079] Among them, the relevant settings of the water sprinkler system:

[0080] Specifically, the water spray system includes a spray device 33 .

[0081] Specifically, a spray device 33 is disposed in each of the first sand box 10 and the second sand box 20 , and a water inlet of the spray device 33 is connected and communicated with an outlet of the water storage barrel 40 through a pipeline.

[0082] Among them, the relevant settings of the wastewater recovery device:

[0083] Specifically, the wastewater recovery device includes a wastewater collection pretreatment barrel 50 and a wastewater treatment system 51; the input port of the wastewater collection pretreatment barrel 50 is connected and communicated with each first water outlet through a pipeline, and a water outlet flow control valve 52 can be set on the pipeline. The output port of the wastewater collection pretreatment barrel 50 is connected and communicated with the input port of the wastewater treatment system 51. The wastewater treatment system 51 is used to purify the wastewater, and the output port of the wastewater treatment system 51 is communicated with the water storage barrel 40.

[0084] Among them, regarding other related settings:

[0085] Among the optional solutions of this embodiment, it is more preferred that Figure 1 to Figure 3 and Figure 6 As shown, the monitoring well group 14 and the injection well group 15 both include a plurality of well groups, including deep wells and shallow wells; the upper ends of the deep wells and the shallow wells are both higher than the upper plane of the soil layer in the first sand box 10; the lower end of the deep well is connected to the groundwater in the first sand box 10; the lower end of the shallow well is located in the soil layer in the first sand box 10. The monitoring well group 14 and the injection well group 15 both include a plurality of well groups consisting of deep wells and shallow wells, which can realize the monitoring of various parameters of different positions of soil and groundwater and the addition of additives.

[0086] Specifically, the monitoring well groups 14 and the injection well groups 15 are evenly distributed in the first sand box 10, and the baffle 11 is disposed at a position capable of separating it into two identical parts.

[0087] Among the optional solutions of this embodiment, it is more preferred that Figure 7 As shown, the lower ends of the deep well and the shallow well are provided with a screen tube 17, the side wall of the screen tube 17 is provided with screen holes, a filter mesh tube 171 is sleeved outside the screen tube 17, and gravel 172 is filled between the screen tube 17 and the filter mesh tube 171. The provided screen tube 17, gravel 172 and filter mesh tube 171 can effectively reduce the soil blocking the screen holes and ensure good connectivity and monitoring status.

[0088] Specifically, the screen pipe 17 of the shallow well corresponds to a depth of 40 cm to 120 cm in the first sand box 10, and the screen pipe 17 of the deep well corresponds to a depth of 120 cm to 200 cm in the first sand box 10. The deep well and the shallow well are well pipes with an inner diameter of 2 cm.

[0089] Specifically, the sieve holes are formed by horizontal knife cutting, with a length of about 1 cm and a seam width of 2 to 3 mm; a cylindrical wire cage with an inner diameter of 4 cm (i.e., filter mesh tube 171) is arranged outside the sieve tube 17, and its pore size is less than 2 mm; the gravel 172 filled between the sieve tube 17 and the filter mesh tube 171 is quartz sand of 4 mm to 7 mm.

[0090] Among the optional solutions of this embodiment, it is more preferred that Figure 1 and Figure 2 As shown, the top opening of the first sand box 10 is also provided with an openable and closable heat preservation cover 31. The heat preservation cover 31 can be closed when the ambient temperature is too low or too high, providing a good temperature environment inside the first sand box 10, ensuring a healthy growth environment for the hyperaccumulator plants 18 in the first sand box 10, and ensuring effective control of water evaporation in the first sand box 10.

[0091] Among the optional solutions of this embodiment, it is more preferred that Figure 1 and Figure 2 As shown, a lighting system 32 that can be raised and lowered is provided above the top opening of the first flask 10. The lighting system 32 can provide a sunlight-simulating environment in each flask, and can simulate different sunlight effects during the day by raising and lowering.

[0092] Among them, the relevant settings about the controller:

[0093] Specifically, the water sprinkler system and the wastewater recovery device are respectively provided with an inlet flow sensor and an outlet flow sensor; the soil parameter monitoring system includes sensors such as soil pH value, temperature, humidity, salinity, N / P / K content, etc.; the water quality parameter monitoring system includes sensors such as groundwater level, groundwater pH value, groundwater conductivity, and groundwater dissolved oxygen.

[0094] Specifically, the controller is a PLC controller 60; the PLC controller 60 can be connected to the computer or the visualization platform 34 via a router or an Ethernet switch; and the PLC controller 60 is connected to both the display device and the printer.

[0095] Specifically, an inlet flow control valve 41 is provided on the output pipeline of the water storage bucket 40, and an outlet flow control valve 52 is provided on the connecting pipeline of the wastewater recovery device; specifically, the inlet flow control valve 41 and the outlet flow control valve 52 can both regulate the flow size and monitor the flow data.

[0096] Specifically, the modules that can be controlled by the PLC controller 60 mainly include but are not limited to the lighting system 32 that simulates sunlight for supplementary lighting, the water spray system, the feeding system for feeding various types of repair agents / microbial agents (each deep well and shallow well of the injection well group 15 is equipped with an automatic feeding device, and the automatic feeding device is any existing device that can feed, and the feeding is controlled by the feeding system), the wastewater treatment pump flow reuse system 53 (the wastewater treatment pump flow reuse system 53 is used to return the water treated by the wastewater treatment system 51 to the water storage tank 40, and can be an extraction pump), the groundwater extraction control system (the deep well and shallow well of each extraction well group 16 are equipped with a water pump, and the groundwater extraction control system is used to control the water pump), and the water inlet flow control valve 41 and the water outlet flow control valve 52 in the water inlet and outlet modules.

[0097] Specifically, the PLC controller 60 is also connected to various sensor modules, including but not limited to water inlet and outlet quantitative sensors, soil pH, nutrient and other sensors, soil temperature and humidity sensors, groundwater multi-parameter sensors (including but not limited to groundwater level, groundwater pH value, groundwater conductivity, groundwater dissolved oxygen, etc.), etc.

[0098] Specifically, the PLC controller 60 is also connected to a data input module, which specifically includes but is not limited to inlet and outlet water quality control input, soil parameter monitoring frequency input, groundwater parameter monitoring frequency input, etc.

[0099] Specifically, the display device is provided with a user management layer, an execution control layer and a data acquisition and display layer; wherein the user management layer specifically includes but is not limited to control switches, power indicator lights, alarm lights or alarm buttons, reset buttons, emergency stop buttons, etc.; wherein the execution control layer specifically includes but is not limited to lighting system 32, temperature control system, water spray system, feeding system, wastewater treatment pump flow reuse system 53, groundwater extraction control system, water inlet flow control valve 41, water outlet flow control valve 52, the switch of the water pump in the wastewater treatment pump flow reuse system 53, water inlet quantitative speed control, water outlet quantitative speed control, etc.; wherein the data acquisition and display layer specifically includes but is not limited to inlet and outlet water volume, inlet and outlet flow rate, groundwater level, soil salinity, soil temperature, soil moisture, soil pH, soil N / P / K content, groundwater pH, groundwater conductivity, groundwater dissolved oxygen, etc. Specifically, Fig. 9 shown.

[0100] Among them, regarding other instructions:

[0101] Specifically, the simulated remediation system 100 based on plant-enhanced microbial remediation of this embodiment can also rely on existing relevant settings and add different remediation agents to conduct remediation research on groundwater or soil alone.

[0102] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A simulated remediation system based on plant-enhanced microbial remediation, characterized in that: including a first sand box; A soil layer is contained in the first sand box, and the surface soil of the soil layer contains composite pollutants, and the composite pollutants include organic pollutants and heavy metal pollutants; A water inlet group is provided on one side of the first sand box, and a water outlet group is provided on the other side of the first sand box opposite thereto, the water inlet group is higher than the water outlet group; the water inlet group is connected to a water supply device, and the water outlet group is connected to a wastewater recovery device; the water supplied by the water supply device forms groundwater at the bottom of the first sand box; A baffle is fixedly arranged in the first sand box, and the baffle separates the topsoil of the soil layer in the first sand box into a plant inoculant area and a plant area, and the plant inoculant area and the plant area have the same area size; The plant inoculant area is added with a repairing microbial agent, and the plant inoculant area and the plant area are both planted with the same hyperaccumulator plants; The plant inoculant area and the plant area are both provided with the same monitoring well group, injection well group and extraction well group; the monitoring well group is provided with monitoring probes of the soil monitoring system and the groundwater monitoring system; the injection well group is provided with a reagent injection hole for communicating with the interior of the first sand box; the extraction well group is provided with an extraction port communicating with the interior of the first sand box; The plant inoculant area and the plant area both include two planting areas and a non-rhizosphere area, the non-rhizosphere area is located between the two planting areas, and the planting area is used to plant the hyperaccumulator plants; The non-rhizosphere area and the adjacent planting area are separated by a first gauze; The water inlet group includes two first water inlets; the water outlet group includes two first water outlets; One end of each of the non-rhizosphere zones is connected and communicated with one of the first water inlets, and the other end of each of the non-rhizosphere zones is connected and communicated with one of the first water outlets.

2. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1 is characterized in that: The monitoring well group and the injection well group both include a plurality of well groups, and the well groups include deep wells and shallow wells; The upper ends of the deep well and the shallow well are both higher than the upper plane of the soil layer in the first sand box; The lower end of the deep well is connected to the groundwater in the first sand box; the lower end of the shallow well is located in the soil layer in the first sand box.

3. The simulated remediation system based on plant-enhanced microbial remediation according to claim 2 is characterized in that: The lower ends of the deep well and the shallow well are both provided with a screen tube, the side wall of the screen tube is provided with screen holes, a filter mesh tube is sleeved on the outer side of the screen tube, and gravel is filled between the screen tube and the filter mesh tube.

4. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1, characterized in that: The hyperaccumulator plants include Medicago sativa, Leucaena leucaena and Sedum serrata; A row of Leucaena leucophylla is planted near the non-rhizosphere area in the planting area, and a row of alfalfa and a row of Sedum serrata are planted on the side of Leucaena leucophylla away from the non-rhizosphere area; The hyperaccumulator plants planted in the two planting areas located on both sides of the non-rhizosphere area are symmetrical to each other.

5. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1, characterized in that: At least one side wall of the first flask is made of transparent material.

6. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1 is characterized in that: The top opening of the first sand box is also provided with a heat-insulating cover that can be opened and closed.

7. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1 is characterized in that: A lighting system that can be raised and lowered is arranged above the top opening of the first sand box.

8. The simulated remediation system based on plant-enhanced microbial remediation according to claim 1 is characterized in that: The first sand box is fixedly arranged on the stand; The water supply device includes a water storage bucket and a water inlet flow control valve. The position of the water storage bucket is higher than the height of the first sand box. The output port of the water storage bucket is connected and communicated with the water inlet group through a connecting pipeline. The water inlet flow control valve is arranged on the connecting pipeline, and the water inlet flow control valve is used to control the flow size of the fluid in the connecting pipeline.

9. The simulated remediation system based on plant-enhanced microbial remediation according to claim 8, characterized in that: A second sand box is fixedly arranged on the stand, and the second sand box serves as a blank control group of the first sand box.

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