A biological cell culture device and a method of using the same

Abstract

The present invention relates to the field of biological cell culture technology, specifically to a biological cell culture device and a method for using the same, including a culture box, wherein a door is rotatably connected to one side of the upper edge of the culture box, and a rotating shaft is rotatably arranged inside the culture box. The biological cell culture device and the method for using the same drive the rotating shaft to drive the bracket to rotate, so that different trays are close to the door. During this process, the connecting pins on both sides of the tray move along the limiting groove, and the slide is used to adaptively extend and retract along the bracket. Compared with the existing culture dish placement parts distributed along the circumferential trajectory, the above-mentioned limiting groove cooperates with the slide, so that the trays can be adjusted in a circular motion, so that different trays are mainly located at the position of the vertical groove body of the limiting groove, that is, the trays are divided into two groups with equal spacing, and the operator only needs to observe and operate all the culture dishes at two distances, without having to find and determine the position of the culture dishes every time.

Description

Technical Field

[0001] The present invention relates to the technical field of biological cell culture, and in particular to a biological cell culture device and a use method thereof. Background Art

[0002] Constant temperature incubator is a general term for a type of constant temperature chamber. It is widely used in medical and health care, pharmaceutical industry, biochemistry, industrial production, agricultural science and other scientific research departments. Its main function is to cultivate various microorganisms or organisms such as tissues and cells.

[0003] The existing patent (publication number: CN117511735A) discloses a biological cell culture device, which relates to the field of biological cell culture technology, including a control component for adjusting the cell culture environment, the control component adjusts the position of multiple groups of culture dishes, and controls the humidity of the culture environment at the same time. A transfer component for picking up the culture dishes is arranged on the side of the control component, and the transfer component firmly clamps the culture dishes to prevent the culture dishes from falling during the transfer process. A liquid replacement component for changing the liquid in the culture dishes is arranged on the transfer component, and the liquid replacement component can adjust the syringe at multiple angles and automatically replace the nozzle on the syringe. A shaking component for shaking the culture dishes is arranged on the side of the control component. The culture dishes are arranged in a circular array inside the above-mentioned existing culture device, and different culture dishes are at different distances from the box door, which makes it inconvenient to take and put, and they can only be put in after being taken out, and they can only be taken and put one by one, which is inconvenient to use.

[0004] In view of this, we propose a biological cell culture device and a method for using the same. Summary of the invention

[0005] The object of the present invention is to provide a biological cell culture device and a method of using the same, so as to solve the problem in the above background technology that culture dishes are arranged in a circular array inside the existing culture device, different culture dishes are at different distances from the door of the box, which is inconvenient to take and put, and they can only be put in after being taken out, and taking and putting can only be done one by one, which is inconvenient to use. In order to achieve the above purpose, the present invention provides the following technical solutions: a biological cell culture device, including an incubator, the incubator has a door rotatably connected to one side along the upper edge, and the incubator is rotatably provided with a rotating shaft inside, two brackets are fixedly installed on the rotating shaft, and the brackets are arranged in a plurality of groups, and the plurality of groups of the brackets are distributed in a circular manner on the rotating shaft, and a tray for placing the culture dishes is arranged between the single groups of brackets, and an arrayer is arranged in the tray, the bracket and the incubator.

[0006] The array device includes a slide groove opened on the bracket, and a slide is slidably connected in the slide groove. The tray is arranged between the slides on both sides, and connecting pins are fixedly arranged on both sides of the tray. The tray is rotatably connected to the slide through the connecting pins, and a counterweight block is fixedly installed on the tray to keep itself horizontal.

[0007] Lining plates are fixedly mounted on the inner walls on both sides of the incubator, and rectangular limiting grooves are provided on the surfaces of the lining plates. The ends of the connecting pins penetrate the slide and extend into the limiting grooves.

[0008] The box door, the lining plate and the limiting groove are provided with a release component.

[0009] Preferably, the release assembly includes a through opening opened on the box door, and a movable cover that closes the through opening is slidably connected to the outer side of the box door. The groove body of the limiting groove close to the side of the box door is set as a horizontal V-shaped offset groove, and the position of the offset groove corresponds to the through opening.

[0010] A circular groove for placing a culture dish is provided on the surface of the tray, and a plurality of inverted teeth for limiting the downward movement of the culture dish are rotatably connected to the inner side wall of the circular groove.

[0011] A socket is fixedly installed on the inner side of the movable cover, and a slot corresponding to the tray is opened on the side surface of the socket, an upper groove corresponding to the circular groove is opened on the upper side of the slot, and a removal groove penetrating the socket and the box door is opened on the inner side wall of the upper groove.

[0012] The inner top wall of the slot is provided with an upper rotating slot connected to the upper slot, and an upper toothed disc is rotatably connected in the upper rotating slot, an upper spiral strip is fixedly connected to the bottom of the upper toothed disc, and one side of the upper spiral strip is located in the upper slot and the slot.

[0013] The inner top wall of the slot is provided with an upper shaft hole connected to the upper rotating groove, and a driving shaft is arranged in the upper shaft hole. The top end of the driving shaft is provided with upper teeth meshing with the upper gear plate, and the surface of the driving shaft located in the slot is provided with middle teeth.

[0014] A notch matched with the driving shaft and the upper spiral strip is formed on the side surface of the tray, and a tooth edge matched with the middle tooth pattern is formed on the side wall of the notch.

[0015] The notch is communicated with the circular groove.

[0016] The socket and the driving shaft are provided with a loading assembly.

[0017] Preferably, the loading assembly includes a lower groove opened along the lower side of the slot and corresponding to the circular groove, and a loading groove penetrating the socket and the box door is opened on the inner side wall of the lower groove.

[0018] The inner bottom wall of the slot is provided with a lower rotating slot connected to the lower slot, and a lower toothed disc is rotatably connected in the lower rotating slot, a lower spiral strip is fixedly connected to the top of the lower toothed disc, and one side of the lower spiral strip is located in the lower slot and the slot.

[0019] The inner bottom wall of the slot is provided with a lower shaft hole connected with the lower rotating groove, the bottom end of the driving shaft extends into the lower shaft hole, and the bottom end of the driving shaft is provided with lower teeth meshing with the lower gear plate.

[0020] Preferably, the movable cover is provided with a locking bolt abutting against the box door.

[0021] Preferably, the bottom wall of the extraction groove is configured as an inclined surface, and the highest position of the inclined surface is located at one side of the upper groove.

[0022] Preferably, the tray is provided with a limit bar which restricts the inverted teeth in a horizontal state from rotating downward.

[0023] Preferably, the slot wall of the slot is configured as an inclined wall adapted to the motion trajectory of the tray.

[0024] A method for using a biological cell culture device comprises the following steps:

[0025] S1. Place the biological cells to be cultured in a culture dish, and then place the culture dish on a tray. When the culture dish needs to be placed on different trays, the drive shaft drives the bracket to rotate so that different trays are close to the door. During this process, the connecting pins on both sides of the tray move along the limit grooves, and the slide is used to adaptively extend and retract along the bracket;

[0026] S2. When the culture dish needs to be taken out, the socket is moved down by using the movable cover. At this time, the slot is aligned with the offset slot. When the tray reaches the box door, the connecting pin slides into the offset slot along the limiting slot, so that the tray moves toward the socket and is inserted into the slot. During this process, the tooth edge along the middle tooth pattern on the notch pushes the drive shaft to rotate, and the drive shaft drives the upper spiral strip to rotate synchronously along the upper toothed disc, so that the upper spiral strip in the notch drives the culture dish in the circular groove to move up to the upper slot, and enters the removal slot to detach from the incubator;

[0027] S3. In the process of the tooth pattern in the tooth edge pushing the driving shaft to rotate, for the empty circular groove, the culture dish to be placed in the incubator is pushed into the lower groove along the loading groove. At this time, the rotating driving shaft pushes the lower spiral strip to rotate along the lower tooth disc, and pushes the culture dish in the lower groove to move up into the circular groove on the tray. The culture dish pushes away the inverted teeth and is carried by the inverted teeth.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] In the present invention, the bracket is driven to rotate by driving the rotating shaft to make different trays approach the box door. During this process, the connecting pins on both sides of the tray move along the limiting grooves, and the slide is used to adaptively extend and retract along the bracket. Compared with the existing culture dish placement parts distributed along a circular trajectory, the above-mentioned limiting grooves cooperate with the slide to enable the trays to be mainly located at the vertical groove body of the limiting groove while being able to adjust their positions by circular motion, that is, the trays are divided into two groups with equal distances. The operator only needs to observe and operate all the culture dishes at two distances without having to find and determine the position of the culture dishes every time.

[0030] In the present invention, the tray is displaced toward the socket and inserted into the slot by sliding the connecting pin along the limiting slot. During this process, the tooth pattern along the tooth edge of the notch pushes the drive shaft to rotate, and the drive shaft drives the upper spiral strip to rotate synchronously along the upper toothed disk, so that the upper spiral strip in the notch drives the culture dish in the circular groove to move up into the upper groove, and enters the removal slot to detach from the incubator. Compared with the existing operator opening the device to take out the culture dishes one by one, the above-mentioned tray cooperates with the upper spiral strip, and the tray can be used to cooperate with the socket, so that the docking and insertion process of the tray can automatically push out the culture dish inside. On the one hand, there is no need for the operator to open and probe into the incubator to operate, which reduces the possibility of contamination to the inside of the incubator. On the other hand, batch and mechanized removal operations can improve operating efficiency.

[0031] In the present invention, the culture dish to be placed in the incubator is pushed into the lower groove along the loading groove. At this time, the rotating driving shaft drives the lower spiral strip to rotate along the lower gear plate, and pushes the culture dish in the lower groove to move up and enter the circular groove on the tray. The culture dish pushes against the inverted teeth and is carried by the inverted teeth. Compared with the existing opening equipment that loads the culture dishes one by one, the loading point of the above-mentioned culture dish is fixed at the loading groove and can be placed along all the trays without position deviation, and the operation is more convenient. Moreover, the removal process of the upper spiral strip can be carried out synchronously with the loading process of the lower spiral strip. There is no need to wait for the removal before proceeding to the next step, which optimizes the operation steps and has good operation convenience. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0033] Figure 2 It is a schematic diagram of the structure of the liner and the pallet of the present invention;

[0034] Figure 3 The exploded view of the liner plate, the rotating shaft, the bracket and the tray of the present invention;

[0035] Figure 4 It is a structural schematic diagram of the box door and the movable cover of the present invention;

[0036] Figure 5It is a three-dimensional structural cross-sectional view of the socket and the tray of the present invention;

[0037] Figure 6 The three-dimensional structure of the socket and the upper and lower grooves of the present invention is cut away Figure 1 ;

[0038] Figure 7 The three-dimensional structure of the socket and the upper and lower grooves of the present invention is cut away Figure 2 ;

[0039] Figure 8 It is an exploded view of the upper rotating groove, the lower rotating groove, the upper spiral strip and the lower spiral strip of the present invention.

[0040] In the figure: 1, incubator; 2, door; 3, shaft; 4, bracket; 5, tray; 6, arrayer; 61, slide; 62, slide; 63, connecting pin; 64, counterweight; 65, lining plate; 66, limit slot; 67, release assembly; 671, through port; 672, movable cover; 673, offset slot; 674, round slot; 675, inverted tooth; 676, socket; 677, slot; 678, upper slot; 679, removal slot; 6710, upper Rotating groove; 6711, upper toothed disc; 6712, upper spiral strip; 6713, upper shaft hole; 6714, driving shaft; 6715, upper tooth pattern; 6716, middle tooth pattern; 6717, notch; 6718, tooth edge; 6719, loading component; 67191, lower groove; 67192, loading groove; 67193, lower rotating groove; 67194, lower toothed disc; 67195, lower spiral strip; 67196, lower shaft hole; 67197, lower tooth pattern. DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technical staff in this field without creative work are within the scope of protection of the present invention.

[0042] See also Figures 1 to 8The present invention provides a technical solution: a biological cell culture device, comprising a culture box 1, a box door 2 is rotatably connected along one side of the culture box 1, and a rotating shaft 3 is rotatably arranged inside the culture box 1, two brackets 4 are fixedly installed on the rotating shaft 3, and the brackets 4 are arranged in a plurality of groups, and the plurality of groups of brackets 4 are annularly distributed on the rotating shaft 3, and trays 5 for placing culture dishes are arranged between the single groups of brackets 4, biological cells to be cultured are placed in the culture dishes, and then the culture dishes are placed on the trays 5, and when it is necessary to take and place culture dishes on different trays 5, the rotating shaft 3 is driven to drive the brackets 4 to rotate, so that different trays 5 are close to the box door 2, and an arrayer 6 is arranged in the tray 5, the brackets 4 and the culture box 1.

[0043] The arrayer 6 includes a slide groove 61 opened on the bracket 4, and a slide 62 is slidably connected in the slide groove 61. The tray 5 is arranged between the slides 62 on both sides, and connecting pins 63 are fixedly arranged on both sides of the tray 5. The tray 5 is rotatably connected to the slide 62 through the connecting pins 63, and a counterweight block 64 is fixedly installed on the tray 5 to keep itself horizontal.

[0044] Lining plates 65 are fixedly installed on the inner walls on both sides of the incubator 1, and a rectangular limiting groove 66 is opened on the surface of the lining plate 65. The end of the connecting pin 63 passes through the slide 62 and extends into the limiting groove 66. During the process of driving the rotating shaft 3 to drive the bracket 4 to rotate, the connecting pins 63 on both sides of the tray 5 move along the limiting groove 66, and the slide 62 is used to adaptively extend and retract along the bracket 4, so that different trays 5 are mainly located at the vertical groove body of the limiting groove 66, that is, the trays 5 are divided into two groups with equal distances.

[0045] A release assembly 67 is provided on the box door 2 , the lining plate 65 and the limiting groove 66 .

[0046] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8 As shown, the release assembly 67 includes a through opening 671 opened on the box door 2, and a movable cover 672 that closes the through opening 671 is slidably connected to the outer side of the box door 2. The groove body of the limiting groove 66 close to the side of the box door 2 is set as a horizontal V-shaped offset groove 673, and the position of the offset groove 673 corresponds to the through opening 671.

[0047] A circular groove 674 for placing a culture dish is formed on the surface of the tray 5 , and a plurality of inverted teeth 675 for limiting the downward movement of the culture dish are rotatably connected to the inner side wall of the circular groove 674 .

[0048] A socket 676 is fixedly installed on the inner side of the movable cover 672, and a slot 677 corresponding to the tray 5 is provided on the side surface of the socket 676. The socket 676 is moved downward by using the movable cover 672 so that the slot 677 is aligned with the offset groove 673. A flap can be provided at the notch of the slot 677, which is directly pushed open when the tray 5 is inserted, and after the tray 5 is pulled out, the flap closes the slot 677. When the tray 5 reaches the box door 2, the connecting pin 63 slides into the offset groove 673 along the limiting groove 66, so that the tray 5 is displaced toward the socket 676 and inserted into the slot 677. An upper groove 678 corresponding to the circular groove 674 is provided on the upper side of the slot 677, and a removal groove 679 that passes through the socket 676 and the box door 2 is provided on the inner wall of the upper groove 678.

[0049] The inner top wall of the slot 677 is provided with an upper rotating slot 6710 connected to the upper slot 678 , and an upper gear plate 6711 is rotatably connected in the upper rotating slot 6710 , an upper spiral strip 6712 is fixedly connected to the bottom of the upper gear plate 6711 , and one side of the upper spiral strip 6712 is located in the upper slot 678 and the slot 677 .

[0050] The inner top wall of the slot 677 is provided with an upper shaft hole 6713 which is connected to the upper rotating groove 6710, and a driving shaft 6714 is arranged in the upper shaft hole 6713. The top end of the driving shaft 6714 is provided with an upper tooth pattern 6715 which meshes with the upper gear plate 6711, and the surface of the driving shaft 6714 located in the slot 677 is provided with a middle tooth pattern 6716.

[0051] A notch 6717 matching with the driving shaft 6714 and the upper spiral strip 6712 is formed on the side surface of the tray 5 , and a toothed edge 6718 matching with the middle tooth pattern 6716 is formed on the side wall of the notch 6717 .

[0052] The notch 6717 is connected to the circular groove 674. When the tray 5 is inserted into the slot 677, the toothed edge 6718 on the notch 6717 pushes the driving shaft 6714 to rotate along the middle tooth pattern 6716, and the driving shaft 6714 drives the upper spiral strip 6712 to rotate synchronously along the upper toothed disc 6711, so that the upper spiral strip 6712 in the notch 6717 drives the culture dish in the circular groove 674 to move up to the upper groove 678, and enter the removal groove 679 to detach from the incubator 1. The movable cover 672 and the socket 676 can also be set to multiple corresponding to the circular groove 674 on a single tray 5, so that the culture dishes in different circular grooves 674 on a single tray 5 can be selectively removed separately.

[0053] An insertion assembly 6719 is provided on the socket 676 and the drive shaft 6714 .

[0054] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8 As shown, the loading component 6719 includes a lower groove 67191 opened along the lower side of the slot 677 and corresponding to the circular groove 674, and a loading groove 67192 penetrating the socket 676 and the box door 2 is opened on the inner side wall of the lower groove 67191.

[0055] The inner bottom wall of the slot 677 is provided with a lower rotating groove 67193 connected to the lower groove 67191, and a lower toothed disc 67194 is rotatably connected in the lower rotating groove 67193, a lower spiral bar 67195 is fixedly connected to the top of the lower toothed disc 67194, and one side of the lower spiral bar 67195 is located in the lower groove 67191 and the slot 677.

[0056] The inner bottom wall of the slot 677 is provided with a lower shaft hole 67196 connected to the lower rotating groove 67193, the bottom end of the driving shaft 6714 extends into the lower shaft hole 67196, and the bottom end of the driving shaft 6714 is provided with a lower tooth pattern 67197 meshing with the lower tooth disk 67194. The culture dish to be placed in the incubator 1 is pushed into the lower groove 67191 along the loading groove 67192. At this time, the rotating driving shaft 6714 pushes the lower spiral bar 67195 to rotate along the lower tooth disk 67194, and pushes the culture dish in the lower groove 67191 to move up into the circular groove 674 on the tray 5. The culture dish pushes against the inverted teeth 675 and is carried by the inverted teeth 675.

[0057] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8 As shown, the movable cover 672 is provided with a locking bolt that abuts against the box door 2. When the locking bolt is tightened, the bolt abuts against the box door 2 to lock the movable cover 672 so that it cannot move. After loosening the locking bolt, the movable cover 672 can move up and down along the box door 2.

[0058] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8 As shown, the bottom wall of the removal groove 679 is set as an inclined surface, and the high position of the inclined surface is located on the side of the upper groove 678. The culture dish raised to the position of the removal groove 679 by the upper spiral strip 6712 can slide out along the inclined surface.

[0059] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8As shown, the tray 5 is provided with a limit bar which restricts the inverted teeth 675 in the horizontal state from rotating downward. When the culture dish rises from the bottom of the circular groove 674 and enters the circular groove 674, the inverted teeth 675 can be pushed to rotate upward, and then the inverted teeth 675 return to the horizontal state to carry the culture dish.

[0060] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figures 5 to 8 As shown, the slot wall of the slot 677 is set to be an inclined wall adapted to the movement trajectory of the tray 5. When the tray 5 moves horizontally through the offset slot 673, there will be a certain amount of up and down movement simultaneously. The inclined wall allows the tray 5 to be accurately inserted into the slot 677.

[0061] A method for using a biological cell culture device comprises the following steps:

[0062] S1. Place the biological cells to be cultured in a culture dish, and then place the culture dish on the tray 5. When it is necessary to take and place the culture dishes on different trays 5, drive the rotating shaft 3 to drive the bracket 4 to rotate, so that different trays 5 are close to the box door 2. During this process, the connecting pins 63 on both sides of the tray 5 move along the limiting groove 66, and the slide 62 is used to adaptively extend and retract along the bracket 4, so that different trays 5 are mainly located at the position of the vertical groove body of the limiting groove 66, that is, the tray 5 is divided into two groups with equal distances.

[0063] S2. When the culture dish needs to be taken out, the socket 676 is moved downward by the movable cover 672. At this time, the slot 677 is aligned with the offset slot 673. When the tray 5 reaches the door 2, the connecting pin 63 slides into the offset slot 673 along the limiting slot 66, so that the tray 5 moves toward the socket 676 and is inserted into the slot 677. During this process, the toothed edge 6718 on the notch 6717 pushes the drive shaft 6714 to rotate along the middle tooth pattern 6716, and the drive shaft 6714 drives the upper spiral strip 6712 to rotate synchronously along the upper toothed disc 6711, so that the upper spiral strip 6712 in the notch 6717 drives the culture dish in the circular groove 674 to move upward into the upper groove 678, and enters the removal slot 679 to detach from the incubator 1.

[0064] S3. In the process of the tooth edge 6718 pushing the driving shaft 6714 to rotate along the middle tooth pattern 6716, for the empty circular groove 674, the culture dish to be placed in the incubator 1 is pushed into the lower groove 67191 along the loading groove 67192. At this time, the rotating driving shaft 6714 pushes the lower spiral strip 67195 to rotate along the lower tooth disc 67194, and pushes the culture dish in the lower groove 67191 to move up into the circular groove 674 on the tray 5. The culture dish pushes against the inverted teeth 675 and is carried by the inverted teeth 675.

[0065] The above shows and describes the basic principles, main features and advantages of the present invention. Technical personnel in this industry should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A biological cell culture device, comprising a culture box (1), Features: The incubator (1) is rotatably connected to a door (2) on one side, and a rotating shaft (3) is rotatably arranged inside the incubator (1), and two brackets (4) are fixedly mounted on the rotating shaft (3), and the brackets (4) are arranged in a plurality of groups, and the plurality of groups of the brackets (4) are annularly distributed on the rotating shaft (3), and trays (5) for placing culture dishes are arranged between the single groups of brackets (4), and array devices (6) are arranged inside the tray (5), the brackets (4) and the incubator (1); The array device (6) comprises a slide groove (61) provided on the bracket (4), and a slide frame (62) is slidably connected in the slide groove (61), the tray (5) is arranged between the slide frames (62) on both sides, and connecting pins (63) are fixedly arranged on both sides of the tray (5), the tray (5) is rotatably connected to the slide frame (62) via the connecting pins (63), and a counterweight block (64) for maintaining the tray (5) is fixedly installed on the tray (5); Lining plates (65) are fixedly mounted on the inner walls of the left and right sides of the incubator (1), and a rectangular limiting groove (66) is provided on the surface of the lining plate (65), and the end of the connecting pin (63) passes through the slide frame (62) and extends into the limiting groove (66); The box door (2), the lining plate (65) and the limiting groove (66) are provided with a release component (67); The release assembly (67) comprises a through opening (671) formed on the box door (2), and a movable cover (672) is slidably connected to the outer side of the box door (2) and closes the through opening (671); the groove body of the limiting groove (66) on the side close to the box door (2) is configured as a horizontally placed V-shaped offset groove (673), and the position of the offset groove (673) corresponds to the through opening (671); The surface of the tray (5) is provided with a circular groove (674) for placing a culture dish, and the inner side wall of the circular groove (674) is rotatably connected to a plurality of inverted teeth (675) for limiting the downward movement of the culture dish; A socket (676) is fixedly mounted on the inner side of the movable cover (672), and a slot (677) corresponding to the tray (5) is provided on the side surface of the socket (676), an upper slot (678) corresponding to the circular slot (674) is provided on the upper side of the slot (677), and a removal slot (679) penetrating the socket (676) and the box door (2) is provided on the inner side wall of the upper slot (678); An upper rotating groove (6710) communicating with the upper groove (678) is formed on the inner top wall of the slot (677), and an upper toothed disc (6711) is rotatably connected in the upper rotating groove (6710), an upper spiral strip (6712) is fixedly connected to the bottom of the upper toothed disc (6711), and one side of the upper spiral strip (6712) is located in the upper groove (678) and the slot (677); An upper shaft hole (6713) communicating with the upper rotating groove (6710) is formed on the inner top wall of the slot (677), and a driving shaft (6714) is disposed in the upper shaft hole (6713). An upper tooth pattern (6715) meshing with the upper toothed disc (6711) is formed on the top of the driving shaft (6714), and a middle tooth pattern (6716) is formed on the surface of the driving shaft (6714) located in the slot (677); The side surface of the tray (5) is provided with a notch (6717) that matches the drive shaft (6714) and the upper spiral strip (6712), and the side wall of the notch (6717) is provided with a tooth edge (6718) that matches the middle tooth pattern (6716); The notch (6717) is in communication with the circular groove (674); The socket (676) and the drive shaft (6714) are provided with a loading assembly (6719); The loading assembly (6719) comprises a lower groove (67191) opened along the lower side of the slot (677) and corresponding to the circular groove (674), and a loading groove (67192) penetrating the socket (676) and the box door (2) is opened on the inner side wall of the lower groove (67191); The inner bottom wall of the slot (677) is provided with a lower rotating slot (67193) connected to the lower slot (67191), and a lower toothed disc (67194) is rotatably connected in the lower rotating slot (67193), a lower spiral strip (67195) is fixedly connected to the top of the lower toothed disc (67194), and one side of the lower spiral strip (67195) is located in the lower slot (67191) and the slot (677); The inner bottom wall of the slot (677) is provided with a lower shaft hole (67196) connected to the lower rotating groove (67193), the bottom end of the drive shaft (6714) extends into the lower shaft hole (67196), and the bottom end of the drive shaft (6714) is provided with a lower tooth pattern (67197) that meshes with the lower gear plate (67194).

2. A biological cell culture device according to claim 1, Features: The movable cover (672) is provided with a locking bolt that abuts against the box door (2).

3. A biological cell culture device according to claim 2, Features: The bottom wall of the removal groove (679) is configured as an inclined surface, and the highest position of the inclined surface is located on one side of the upper groove (678).

4. A biological cell culture device according to claim 3, Features: The tray (5) is provided with a limit bar which limits the inverted teeth (675) in a horizontal position so as to prevent them from rotating downward.

5. A biological cell culture device according to claim 4, Features: The slot wall of the slot (677) is configured as an inclined wall adapted to the motion trajectory of the tray (5).

6. The method for using the biological cell culture device according to claim 5, comprising the following steps: S1. placing biological cells to be cultured in a culture dish, and then placing the culture dish on a tray (5). When it is necessary to take or place the culture dish on different trays (5), the drive shaft (3) drives the support (4) to rotate so that different trays (5) are close to the box door (2). During this process, the connecting pins (63) on both sides of the tray (5) move along the limiting groove (66), and the slide (62) is used to adaptively extend and retract along the support (4) so ​​that different trays (5) are mainly located at the vertical groove body of the limiting groove (66), that is, the trays (5) are divided into two groups with equal spacing; S2. When it is necessary to remove the culture dish, the socket (676) is moved downward by using the movable cover (672). At this time, the slot (677) is aligned with the offset slot (673). When the tray (5) reaches the box door (2), the connecting pin (63) slides into the offset slot (673) along the limiting slot (66), so that the tray (5) is displaced toward the socket (676) and inserted into the slot (677). During this process, the toothed edge (6718) on the notch (6717) drives the drive shaft (6714) to rotate along the middle tooth pattern (6716), and the drive shaft (6714) drives the upper spiral strip (6712) to rotate synchronously along the upper toothed disc (6711), so that the upper spiral strip (6712) in the notch (6717) drives the culture dish in the circular groove (674) to move upward into the upper groove (678), and enter the removal groove (679) to be separated from the culture box (1); S3. When the tooth edge (6718) pushes the driving shaft (6714) to rotate along the middle tooth pattern (6716), the culture dish to be placed in the incubator (1) is pushed into the lower groove (67191) along the loading groove (67192) for the empty circular groove (674). At this time, the rotating driving shaft (6714) pushes the lower spiral strip (67195) to rotate along the lower toothed disc (67194), and pushes the culture dish in the lower groove (67191) to move upward into the circular groove (674) on the tray (5). The culture dish pushes against the inverted teeth (675) and is carried by the inverted teeth (675).

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