An organoid-on-a-chip platform for drug testing of acute infectious diseases

Abstract

The present invention discloses an organoid chip platform for acute infectious disease drug testing, which relates to the technical field of drug testing. The platform comprises an experimental group data acquisition module, an experimental data analysis module, a real-time morphological coefficient acquisition module and a distribution abnormality marking module. The real-time position information of a culture fluid to be tested at different channel lines of an organoid chip is obtained in real time, the state change of the culture fluid in the chip is monitored in real time, the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment are obtained, and the real-time morphological coefficients are compared and analyzed with the reference morphological coefficients to determine and generate abnormal markings, thereby solving the problem that the existing platform is difficult to monitor the state change of the culture fluid in real time during drug testing and is unable to timely discover the abnormal flow of the culture fluid in the chip. The abnormal flow during drug testing can be discovered in time, thereby improving the safety and effectiveness of the test.

Description

Technical Field

[0001] The present invention belongs to the technical field of drug testing, and in particular is an organoid chip platform for testing drugs for acute infectious diseases. Background Art

[0002] The prevention and treatment of acute infectious diseases has always been a major challenge in the field of global public health. It is crucial to quickly and accurately test the efficacy of drugs against acute infectious diseases. Traditional drug testing methods often have many limitations, such as long experimental cycles, high costs, and inaccurate results. As an emerging experimental method, organoid chip technology has the advantages of miniaturization, high throughput, and the ability to simulate the human physiological environment, providing a new way to test drugs for acute infectious diseases.

[0003] In this context, organ-on-chips for acute infectious disease drug testing have come into being. Organ-on-chips enable more efficient drug testing, providing strong technical support for the fight against acute infectious diseases. At the same time, with the continuous advancement of science and technology, the accuracy, real-time and reliability of drug testing are increasingly required to meet the needs of practical applications. Organ-on-chips play a greater role in basic research and biomedical applications.

[0004] However, traditional analysis methods of organoid chips may only consider simple position coordinates or flow velocity, ignoring the discreteness and complexity of the culture fluid distribution. During the drug testing process, it is impossible to obtain the state changes of the culture fluid in real time, and it is difficult to timely understand the flow of the culture fluid in the chip, which affects the accuracy and reliability of drug testing and increases the risk of test failure. In order to solve the above problems, an organoid chip platform for acute infectious disease drug testing is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide an information interaction method for power field operations, which solves the technical problem that during the drug testing process, the state changes of the culture solution cannot be obtained in real time, and it is difficult to timely understand the flow of the culture solution in the chip, thereby affecting the accuracy and reliability of the drug testing.

[0006] An organoid chip platform for acute infectious disease drug testing, including;

[0007] The experimental group data acquisition module presets multiple data acquisition moments and sets multiple identical organoid chips, injects the same culture medium under the same experimental environment, records the position information of the culture medium at different channel lines of each organoid chip at different data acquisition moments, and obtains the experimental group data corresponding to the culture medium at each data acquisition moment;

[0008] The experimental data analysis module analyzes the experimental group data corresponding to the culture medium at each data acquisition time, and obtains the reference morphological coefficients corresponding to the culture medium in the organoid chip at each data acquisition time;

[0009] A real-time morphological coefficient acquisition module, which obtains the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment according to the real-time position information corresponding to different channel lines of the culture fluid to be tested on the organoid chip;

[0010] The distribution abnormality marking module compares and analyzes the real-time morphological coefficients corresponding to the tested culture fluid in the organoid chip at each data acquisition moment with the corresponding reference morphological coefficients one by one, and generates a distribution abnormality mark based on the comparison and analysis results.

[0011] As a further solution of the present invention: the organoid chip includes a first substrate, three columns of test groups are arranged on the first substrate, each column of the test groups includes a liquid inlet, a detection pool and three cell culture chambers, and one liquid inlet, one detection pool and three cell culture chambers in each column of the test group are arranged in sequence from top to bottom on the first substrate and connected through microchannels. At the same time, each cell culture chamber in the organoid chip is connected through microchannels, thereby obtaining a connectivity relationship image on the organoid chip.

[0012] As a further solution of the present invention: the specific method of obtaining the experimental group data corresponding to the culture solution at each data acquisition time is:

[0013] Preset n data acquisition moments Tn, and set r identical organoid chips at the same time, inject the same culture medium into each identical organoid chip under the same experimental environment, and obtain and record the position information corresponding to the culture medium in different microchannels of each organoid chip at each data acquisition moment Tn, and mark the position information corresponding to the culture medium in each identical organoid chip at each data acquisition moment Tn as the experimental group data corresponding to the culture medium at each data acquisition moment Tn, wherein n and r are preset values, n is the number corresponding to the data acquisition moment, r is the number of identical organoid chips set, and n and r are both positive integers, n≥1, r≥1.

[0014] As a further solution of the present invention, the specific method of obtaining the position information corresponding to the culture solution in different microchannels of each organoid chip at each data acquisition time is:

[0015] A high-definition camera is placed directly above each organoid chip to obtain an image corresponding to each organoid chip at each data acquisition time Tn, and obtain the position of the culture solution in different microchannels of each organoid chip at each data acquisition time Tn;

[0016] Determine the center points corresponding to the liquid inlet, cell culture chamber and detection pool on the organoid chip, and map them into nodes. Connect each node according to the connectivity image to obtain the control image corresponding to the organoid chip. Label each node from left to right and from top to bottom on the control image to obtain the node label Bi corresponding to each node in the control image, where i refers to different nodes, i=1, 2, ..., a, where a refers to the number of nodes, a≥1, define the line between every two nodes in the control image as a channel line, combine the node labels corresponding to the two nodes constituting each channel line, and obtain the channel number Ce corresponding to each channel line. , where e refers to different channel lines, e=1, 2, ..., b, where b refers to the number of channel lines, b≥1, the connectivity image on the organoid chip and the control image are fused to obtain the organoid chip channel reference image, and the image corresponding to each organoid chip at each data acquisition time Tn is obtained by a high-definition camera device, and the corresponding end position coordinates of the culture medium in the different microchannels of each organoid chip at each data acquisition time Tn are recorded, and the organoid chip channel reference image is referred to to obtain the position information corresponding to the culture medium in the different microchannels of each organoid chip at each data acquisition time, that is, the position information corresponding to different channel lines.

[0017] As a further solution of the present invention: the specific method of obtaining the reference morphological coefficients corresponding to the culture medium in the organoid chip at each data acquisition moment is:

[0018] Step S1: randomly select one of the data acquisition moments as the analysis moment;

[0019] Step S2: randomly selecting one of the organoid chips as the target chip;

[0020] Obtain the end position coordinates Wi (Wxi, Wyi) corresponding to the culture medium at different channel lines of the target chip at the analysis time, and obtain the center point coordinates of the organoid chip channel reference image, and mark it as Z (Zx, Zy). Use the distance calculation formula to calculate the distance Li between each position coordinate Wi and the center point coordinate Z, calculate the discrete value of the distance Li, and mark it as the distribution coefficient W1 corresponding to the culture medium in the target chip at the analysis time;

[0021] Step S3: Repeat step S2 to obtain the distribution coefficient Wr of the culture medium in each organoid chip at the analysis time;

[0022] Step S4: Compare and analyze the number v of the distribution coefficient Wr that meets the preset condition Q: |Wr-Wp|>Y1 with the preset value Y2, and then obtain the reference morphological coefficient H1 corresponding to the organoid chip at the analysis time according to the comparison result;

[0023] Step S5: Repeat steps S1 to S4 to obtain the reference morphological coefficient Hn corresponding to the culture medium in the organoid chip at each data acquisition moment.

[0024] As a further solution of the present invention: the specific method of obtaining the reference morphological coefficient corresponding to the organoid chip at the time of analysis is:

[0025] When v is greater than the preset value Y2, the mean of the distribution coefficient Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the time of analysis; when v is less than or equal to the preset value Y2, the mean of the maximum and minimum values ​​in Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the time of analysis.

[0026] As a further solution of the present invention, the specific method of obtaining the real-time morphological coefficients of the culture fluid to be tested in the organoid chip at each data acquisition time is:

[0027] When conducting drug testing, after the culture fluid to be tested is injected through the liquid inlet, the real-time position information corresponding to the culture fluid to be tested at different channel lines of the organoid chip is obtained in real time according to each data acquisition moment, and it is input into the experimental data analysis module, thereby obtaining the real-time morphological coefficient Kn corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment.

[0028] As a further solution of the present invention: determining and generating a distribution abnormality mark, the specific method is as follows:

[0029] Obtain the absolute value Fn of the difference between each real-time morphological coefficient Kn and its corresponding reference morphological coefficient Hn, obtain the number f where Fn is greater than the preset value Y3, define the ratio between f and n as the deviation coefficient J, and when J is greater than the preset value Y4, generate a distribution abnormality mark, otherwise, do nothing.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention obtains the real-time position information of the culture fluid to be tested at different channel lines of the organoid chip in real time, monitors the state change of the culture fluid in the chip in real time, obtains the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment, compares and analyzes the real-time morphological coefficients with the reference morphological coefficients, determines and generates abnormal marks, solves the problem that the existing platform is difficult to monitor the state change of the culture fluid in real time during drug testing and cannot timely discover the abnormal flow of the culture fluid in the chip, can timely discover the abnormal flow during drug testing, improve the safety and effectiveness of the test, provide timely feedback to researchers, help to quickly adjust the experimental plan, and reduce the risk of experimental failure. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the system framework structure of the present invention;

[0033] Figure 2 is a schematic diagram of the structure of the organoid chip of the present invention;

[0034] Figure 3 This is a schematic diagram of the structure of the organoid chip channel reference image of the present invention;

[0035] In the figure: 11, first substrate; 101, liquid inlet; 102, cell culture chamber; 103, detection pool. DETAILED DESCRIPTION

[0036] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Embodiment 1

[0038] See also Figure 1-Figure 3 , the present application provides an organoid chip platform for acute infectious disease drug testing, comprising;

[0039] The experimental group data acquisition module presets n data acquisition moments Tn, and sets r identical organoid chips at the same time. Under the same experimental environment, the same culture medium is injected into each identical organoid chip, and the position information corresponding to the culture medium in different microchannels of each organoid chip at each data acquisition moment Tn is obtained and recorded, and the position information corresponding to the culture medium in each identical organoid chip at each data acquisition moment Tn is marked as the experimental group data corresponding to the culture medium at each data acquisition moment Tn, wherein n and r are preset values, n is the number corresponding to the data acquisition moment, r is the number of identical organoid chips set, and n and r are both positive integers, n≥1, r≥1;

[0040] The specific method for obtaining the position information corresponding to the culture medium in different microchannels of each organoid chip at each data acquisition time Tn is:

[0041] A high-definition camera is placed directly above each organoid chip, and images corresponding to each organoid chip at each data acquisition time Tn are obtained by the high-definition camera, and positions of culture fluids in different microchannels of each organoid chip at each data acquisition time Tn are obtained and recorded;

[0042] The specific method of recording is:

[0043] The organoid chip includes a first substrate 11, on which three test groups are arranged, each of which includes a liquid inlet 101, a detection pool 103 and three cell culture chambers 102. The liquid inlet 101, the detection pool 103 and the three cell culture chambers 102 in each test group are arranged sequentially from top to bottom on the first substrate 11 and are connected through microchannels. At the same time, each cell culture chamber 102 in the organoid chip is connected through the microchannel, thereby obtaining a connectivity relationship diagram on the organoid chip;

[0044] The center points corresponding to the liquid inlet 101, the detection pool 103 and the cell culture chamber 102 on the organoid chip are obtained, and they are mapped into a node. At the same time, the nodes are compared and connected according to the connectivity image on the organoid chip, so as to obtain the control image corresponding to the organoid chip. On the control image, each node is numbered in the order from left to right and from top to bottom, so as to obtain the node number Bi corresponding to each node in the control image, where i refers to different nodes, i=1, 2, ..., a, where a refers to the number of nodes, a≥1;

[0045] The connection between every two nodes in the reference image is defined as a channel line, and the node labels Bi corresponding to the two nodes constituting each channel line are combined to obtain the channel number Ce corresponding to each channel line, where e refers to different channel lines, e=1, 2, ..., b, where b refers to the number of channel lines, b≥1, and then the node label Bi of each node in the reference image and the channel number Ce corresponding to each channel line are obtained, and the connectivity image on the organoid chip and the reference image are fused to obtain the organoid chip channel reference image;

[0046] The image corresponding to each organoid chip at each data acquisition time Tn is obtained by a high-definition camera device, and the coordinates of the corresponding end positions of the culture solution in different microchannels of each organoid chip at each data acquisition time Tn are recorded, and the corresponding position information of the culture solution at different channel lines of each organoid chip at each data acquisition time Tn is obtained by referring to the organoid chip channel reference image;

[0047] The experimental data analysis module analyzes the experimental group data corresponding to the culture medium at each data acquisition time Tn, and then obtains the reference morphological coefficients corresponding to the culture medium in the organoid chip at each data acquisition time Tn. The specific method is as follows:

[0048] Step S1: randomly select one of the data acquisition moments as the analysis moment;

[0049] Step S2: randomly selecting one of the organoid chips as the target chip;

[0050] Obtain the coordinates of the end positions of the culture solution at different channel lines of the target chip at the time of analysis, and mark them as Wi (Wxi, Wyi);

[0051] At the same time, the coordinates of the center point of the organoid chip channel reference image are obtained and marked as Z (Zx, Zy);

[0052] According to the distance calculation formula, , calculate the distance Li between each position coordinate Wi (Wxi, Wyi) and the center point coordinate Z (Zx, Zy), through the formula: , calculate the discrete value of the distance Li and mark it as the distribution coefficient W1 corresponding to the culture solution in the target chip at the analysis time, where Lj is any one of Li, Lp is the mean value of Li, and a≥i≥1;

[0053] Step S3: Repeat step S2 to obtain the distribution coefficient Wr of the culture medium in each organoid chip at the analysis time;

[0054] Step S4: Compare and analyze the number v of the distribution coefficient Wr that meets the preset condition Q: |Wr-Wp|>Y1 with the preset value Y2, and then obtain the reference morphological coefficient H1 corresponding to the organoid chip at the analysis time according to the comparison result. The specific comparison and analysis method is:

[0055] When v is greater than the preset value Y2, the mean of the distribution coefficient Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the time of analysis; when v is less than or equal to the preset value Y2, the mean of the maximum and minimum values ​​in Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the time of analysis, that is, (Wmin+Wmax) / 2=H1, where Wmin and Wmax are the minimum and maximum values ​​in Wr, respectively. The specific values ​​of the preset values ​​Y1 and Y2 are determined by relevant personnel according to actual needs;

[0056] Step S5: Repeat steps S1 to S4 to obtain the reference morphological coefficient Hn corresponding to the culture medium in the organoid chip at each data acquisition moment;

[0057] By acquiring images of the organoid chip at different data acquisition times through high-definition video equipment, and mapping the chip structure into nodes and channel lines, a channel reference image is obtained, which can accurately record the position information of the culture fluid in different microchannels at each data acquisition time, allowing the experimenter to understand the flow of the culture fluid in the chip in more detail. The distribution coefficient is obtained by calculating the distance and discrete value between the end position coordinates of the culture fluid at different channel lines of the target chip and the coordinates of the center point of the channel reference image, and the reference morphological coefficient corresponding to the culture fluid in the organoid chip at each data acquisition time is determined according to different situations, which is conducive to the subsequent comprehensive analysis of the distribution of the culture fluid in the chip.

[0058] Embodiment 2

[0059] As the second embodiment of the present invention, when the present application is specifically implemented, compared with the first embodiment, the technical solution of the present embodiment is different from the first embodiment only in that the present embodiment further includes a real-time morphology coefficient acquisition module;

[0060] When conducting drug testing, after the culture fluid to be tested is injected through the liquid inlet 101, the real-time position information corresponding to the culture fluid to be tested at different channel lines of the organoid chip is obtained in real time according to each data acquisition time, and the real-time position information is input into the experimental data analysis module, thereby obtaining the real-time morphological coefficient Kn corresponding to the culture fluid to be tested in the organoid chip at each data acquisition time;

[0061] The distribution abnormality marking module compares and analyzes the real-time morphological coefficients Kn corresponding to the culture fluid to be tested in the organoid chip at each data acquisition time with the corresponding reference morphological coefficients Hn one by one, and generates a distribution abnormality mark based on the comparison and analysis results. The specific method is as follows:

[0062] Obtain the absolute value Fn of the difference between each real-time morphological coefficient Kn and its corresponding reference morphological coefficient Hn, obtain the number f of Fn greater than the preset value Y3, define the ratio between f and n as the deviation coefficient J, and generate a distribution abnormality mark when J is greater than the preset value Y4, otherwise no processing is performed;

[0063] The real-time position information of the culture fluid to be tested at different channel lines of the organoid chip is obtained in real time, the state changes of the culture fluid in the chip are monitored in real time, and the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment are obtained. The coefficients are compared and analyzed with the reference morphological coefficients to determine and generate abnormal marks, which solves the problems that the existing platform is difficult to monitor the state changes of the culture fluid in the drug testing process in real time and cannot timely detect the abnormal flow of the culture fluid in the chip. The abnormal flow in the drug testing process can be detected in time, the safety and effectiveness of the test can be improved, and timely feedback can be provided to researchers, which is helpful to quickly adjust the experimental plan and reduce the risk of experimental failure. By accurately calculating the absolute value of the difference Fn and the deviation coefficient J and comparing them with the preset threshold, the accuracy and sensitivity of abnormal judgment are improved, the false alarm and missed alarm can be reduced, and the reliability of the experimental results can be ensured.

[0064] Embodiment 3

[0065] As the third embodiment of the present invention, when the present application is specifically implemented, compared with the first and second embodiments, the technical solution of this embodiment is to combine the solutions of the first and second embodiments mentioned above for implementation.

[0066] The above formulas are all dimensionless and numerical calculations. The formula is a formula for the most recent real situation obtained by collecting a large amount of data and performing software simulation. The preset parameters and thresholds in the formula are set by technicians in this field according to actual conditions.

[0067] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. An organoid chip platform for acute infectious disease drug testing, characterized in that: include; The experimental group data acquisition module presets multiple data acquisition moments and sets multiple identical organoid chips, injects the same culture medium under the same experimental environment, records the position information of the culture medium at different channel lines of each organoid chip at different data acquisition moments, and obtains the experimental group data corresponding to the culture medium at each data acquisition moment; The experimental data analysis module analyzes the experimental group data corresponding to the culture medium at each data acquisition time, and obtains the reference morphological coefficients corresponding to the culture medium in the organoid chip at each data acquisition time; A real-time morphological coefficient acquisition module, which obtains the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment according to the real-time position information corresponding to different channel lines of the culture fluid to be tested on the organoid chip; The distribution abnormality marking module compares and analyzes the real-time morphological coefficients corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment with the corresponding reference morphological coefficients one by one, and generates a distribution abnormality mark based on the comparison and analysis results; The organoid chip comprises a first substrate (11), three columns of test groups are arranged on the first substrate (11), each column of the test groups comprises a liquid inlet (101), a detection pool (103) and three cell culture chambers (102), the liquid inlet (101), the detection pool (103) and the three cell culture chambers (102) in each column of the test groups are arranged in sequence from top to bottom on the first substrate (11) and are connected through microchannels, and at the same time, the cell culture chambers (102) in the organoid chip are connected through microchannels, thereby obtaining a connection relationship image on the organoid chip; The specific method of obtaining the experimental group data corresponding to the culture solution at each data acquisition time is: Preset n data acquisition moments Tn, set r identical organoid chips at the same time, inject the same culture medium into each identical organoid chip under the same experimental environment, obtain and record the position information corresponding to the culture medium in different microchannels of each organoid chip at each data acquisition moment Tn, and mark the position information corresponding to the culture medium in each identical organoid chip at each data acquisition moment Tn as the experimental group data corresponding to the culture medium at each data acquisition moment Tn, wherein n and r are preset values, n is the number corresponding to the data acquisition moment, r is the number of identical organoid chips set, and n and r are both positive integers, n≥1, r≥1; The specific method for obtaining the position information corresponding to the culture solution in different microchannels of each organoid chip at each data acquisition time is: A high-definition camera is placed directly above each organoid chip to obtain an image corresponding to each organoid chip at each data acquisition time Tn, and obtain the position of the culture solution in different microchannels of each organoid chip at each data acquisition time Tn; The center points corresponding to the liquid inlet (101), the cell culture chamber (102) and the detection pool (103) on the organoid chip are determined, and mapped into nodes. The nodes are compared and connected according to the connectivity image, so as to obtain the control image corresponding to the organoid chip. The nodes are numbered in the control image from left to right and from top to bottom to obtain the node numbers Bi corresponding to the nodes in the control image, where i refers to different nodes, i=1, 2, ..., a, where a refers to the number of nodes, a≥1, and the line between every two nodes in the control image is defined as a channel line, and the node numbers corresponding to the two nodes constituting each channel line are combined to obtain the node numbers corresponding to each channel line. The channel number Ce, where e refers to different channel lines, e=1, 2, ..., b, where b refers to the number of channel lines, b≥1, the connectivity image on the organoid chip and the control image are fused to obtain the organoid chip channel reference image, the image corresponding to each organoid chip at each data acquisition time Tn is obtained by a high-definition camera device, the corresponding end position coordinates of the culture solution in different microchannels of each organoid chip at each data acquisition time Tn are recorded, and the corresponding position information of the culture solution in different microchannels of each organoid chip at each data acquisition time is obtained by referring to the organoid chip channel reference image, that is, the corresponding position information at different channel lines; The specific method for obtaining the reference morphological coefficients corresponding to the culture medium in the organoid chip at each data acquisition time is: Step S1: randomly select one of the data acquisition moments as the analysis moment; Step S2: randomly selecting one of the organoid chips as the target chip; Obtain the end position coordinates Wi (Wxi, Wyi) corresponding to the culture solution at different channel lines of the target chip at the analysis time, and obtain the center point coordinates of the organoid chip channel reference image, and mark it as Z (Zx, Zy). Use the distance calculation formula to calculate the distance Li between each position coordinate Wi and the center point coordinate Z, calculate the discrete value of the distance Li, and mark it as the distribution coefficient W1 corresponding to the culture solution in the target chip at the analysis time; Step S3: Repeat step S2 to obtain the distribution coefficient Wr of the culture medium in each organoid chip at the analysis time; Step S4: Compare and analyze the number v of the distribution coefficient Wr that meets the preset condition Q: |Wr-Wp|>Y1 with the preset value Y2, and then obtain the reference morphological coefficient H1 corresponding to the organoid chip at the analysis time according to the comparison result; Step S5: Repeat steps S1 to S4 to obtain the reference morphological coefficient Hn corresponding to the culture medium in the organoid chip at each data acquisition moment; The specific method for obtaining the reference morphological coefficient corresponding to the organoid chip at the time of analysis is: When v is greater than the preset value Y2, the mean of the distribution coefficient Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the analysis time; when v is less than or equal to the preset value Y2, the mean of the maximum and minimum values ​​in Wr is used as the reference morphological coefficient H1 corresponding to the culture medium in the organoid chip at the analysis time; Determine the generation of distribution anomaly markers, specifically: Obtain the absolute value Fn of the difference between each real-time morphological coefficient and its corresponding reference morphological coefficient Hn, obtain the number f where Fn is greater than the preset value Y3, define the ratio between f and n as the deviation coefficient J, and when J is greater than the preset value Y4, generate a distribution abnormality mark, otherwise, do nothing.

2. An organoid chip platform for acute infectious disease drug testing according to claim 1, characterized in that: The specific method for obtaining the real-time morphological coefficients of the culture medium to be tested in the organoid chip at each data acquisition time is as follows: When conducting drug testing, after the culture fluid to be tested is injected through the liquid inlet (101), the real-time position information corresponding to the culture fluid to be tested at different channel lines of the organoid chip is obtained in real time according to each data acquisition moment, and the real-time position information is input into the experimental data analysis module, thereby obtaining the real-time morphological coefficient Kn corresponding to the culture fluid to be tested in the organoid chip at each data acquisition moment.

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