Flow detection device and sample analyzer

Abstract

The application discloses a flow detection device and a sample analyzer. The flow detection device comprises an emission module, a flow detection module and a receiving module. The flow detection module comprises a sample flow channel. The sample flow channel is used for conveying a sample flow to be detected. The emission module is used for emitting a light beam to the sample flow channel to form a light spot on the sample flow channel. The receiving module is used for receiving scattered light scattered by the emitted light beam on the sample flow or fluorescence formed by exciting the sample to perform sample detection analysis. The width of the sample flow channel in a first direction is a target width. The target width is not less than 10 microns and not greater than 25 microns. The long axis length of the light spot is not less than 50 microns and less than 160 microns. Based on the above manner, the accuracy of flow detection can be effectively improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection, in particular to a flow detection device and a sample analyzer. BACKGROUND

[0002] In the prior art, a flow detection device usually comprises at least a transmitting module, a flow detection module and a receiving module, the transmitting module is controlled to emit a light beam to a sample flow of the flow detection module, so as to excite the sample in the sample flow to generate fluorescence, and the receiving module is controlled to receive the fluorescence, and the sample is detected based on the fluorescence.

[0003] The defect of the prior art is that the light spot formed by the light beam emitted by the transmitting module on the flow detection device is small, so that the part of the light spot close to the edge is also likely to irradiate on the sample flow, and the sample is excited to generate fluorescence based on the part of the light beam, which is received by the receiving module. Since the stability of the part of the light spot close to the edge is poor, the signal-to-noise ratio of the fluorescence excited by the part of the light beam is poor, and thus the accuracy of the flow detection is low. SUMMARY

[0004] The technical problem solved by the present application is how to improve the accuracy of flow detection.

[0005] In order to solve the above technical problem, the first technical solution adopted by the present application is a flow detection device, comprising a transmitting module, a flow detection module and a receiving module, the flow detection module comprising a sample flow channel; the sample flow channel is used to transport a sample flow to be detected, the transmitting module is used to emit a light beam to the sample flow channel to form a light spot on the sample flow channel, and the receiving module is used to receive scattered light scattered by the emitted light beam on the sample flow or fluorescence excited by the sample to perform sample detection and analysis; the width of the sample flow channel in a first direction is a target width, the target width is not less than 10 microns and not more than 25 microns, the long axis length of the light spot is not less than 50 microns and less than 160 microns; the flow direction of the sample flow is a second direction, the first direction is perpendicular to the second direction, and the first direction is perpendicular to the emission direction of the light beam, the long axis of the light spot coincides with a straight line in the first direction, and the short axis of the light spot coincides with a straight line in the second direction.

[0006] Among them, the preset minimum value of the ratio of the light intensity of the light spot at the edge of the sample flow channel to the maximum light intensity of the light spot is the minimum edge light intensity ratio, and the target width and the minimum edge light intensity ratio are in a negative correlation.

[0007] The preset minimum value of the long axis length of the light spot is a minimum long axis length, the target half-width is an arithmetic square root of a first value, the first value is a quotient of a second value divided by -2, the second value is a product of a third value and a fourth value, the third value is a square of half of the minimum long axis length, and the fourth value is a natural logarithm of a minimum edge light intensity ratio.

[0008] The minimum edge light intensity ratio is between 90% and 98%.

[0009] The emission module includes a semiconductor laser, and the wavelength range of the light beam emitted by the semiconductor laser is 505 nm to 535 nm.

[0010] The receiving module includes a silicon photomultiplier, and the filter wavelength range of the filter configured for the silicon photomultiplier is 555 nm to 615 nm, and the peak wavelength range of the silicon photomultiplier is 420 nm to 650 nm.

[0011] The flow detection device further includes a lens group including at least one lens, the lens group is located on the light path of the fluorescence, and the lens group is located between the flow detection module and the receiving module, and the distance between the lens group and the flow detection module is not less than 0.5 mm and not greater than 1.5 mm.

[0012] The short axis length of the light spot is greater than 9 microns and less than 18 microns, when the target width is greater than 10 microns and less than 25 microns, the minimum value of the long axis length is not less than 90 microns and not greater than 126 microns, and the maximum value of the long axis length is less than 160 microns, when the target width is 10 microns, the long axis length is not less than 90 microns and less than 160 microns, and when the target width is 25 microns, the long axis length is not less than 126 microns and less than 160 microns.

[0013] To solve the above technical problems, a second technical solution adopted by the present application is a sample analyzer including the flow detection device.

[0014] The sample analyzer is an immune analyzer, and the immune analyzer further includes a sample adding assembly configured to inject the sample and a reagent into a reaction cup, and a reaction assembly configured to react the sample and the reagent in the reaction cup, wherein the reagent includes a plurality of detection microspheres, each type of detection microsphere corresponds to a corresponding detection item, and the sampling module is configured to collect the sample to the flow detection device to obtain a detection result corresponding to each detection item through the flow detection device.

[0015] The beneficial effects of the present application are that, different from the prior art, in the technical solution of the present application, the target width is not less than 10 microns and not more than 25 microns, and the long axis length of the light spot is not less than 50 microns and less than 160 microns. Since the target width is the width of the sample flow channel in the flow detection module, and the minimum long axis length is the minimum value of the long axis length of the light spot formed by the light beam emitted by the emission module on the sample flow channel, based on the above-mentioned manner, the part of the light spot irradiated on the sample flow is the part with smaller light intensity fluctuation amplitude in the whole light spot, that is, the flow detection device can use the more stable part of the light spot to detect the sample flow, thereby improving the accuracy of flow detection. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0017] Figure 1 is a structural schematic diagram of an embodiment of the flow detection device of the present application;

[0018] Figure 2 is a structural schematic diagram of an embodiment of the flow detection module of the present application;

[0019] Figure 3 is one of the light intensity proportion distribution diagrams of the light spot in the first direction of the present application;

[0020] Figure 4 is another of the light intensity proportion distribution diagrams of the light spot in the first direction of the present application;

[0021] Figure 5 is a structural schematic diagram of an embodiment of the sample analyzer of the present application.

[0022] The drawings are as follows: emission module 11, flow detection module 12, sample flow channel 121, receiving module 13, sample analyzer 20, flow detection device 21. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all the structures. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0024] The terms "first", "second", "third", etc. in the present application are only used for descriptive purpose and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second", "third" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative position relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications also change accordingly. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but can optionally include steps or units not listed, or can optionally include other steps or units inherent to the process, method, product or device.

[0025] In this document, the term "embodiment" means that the specific features, structures or characteristics described in connection with the embodiment can be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily mean the same embodiment is referred to, nor does it mean that the embodiments are mutually exclusive or alternative to each other. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0026] The present application proposes a flow detection device, referring to Figure 1 and Figure 2 , Figure 1 is a structural schematic diagram of an embodiment of the flow detection device of the present application, Figure 2 is a structural schematic diagram of an embodiment of the flow detection module of the present application, as shown in Figure 1 and Figure 2 The flow detection device includes a transmitting module 11, a flow detection module 12 and a receiving module 13, the flow detection module 12 includes a sample flow channel 121.

[0027] The sample flow channel 121 is used to transport a sample flow to be detected, the transmitting module 11 is used to transmit a light beam to the sample flow channel 121 to form a light spot on the sample flow channel 121, and the receiving module 13 is used to receive scattered light formed by scattering of the transmitted light beam on the sample flow or fluorescence formed by exciting the sample, so as to perform sample detection analysis.

[0028] The width of the sample flow channel 121 in the first direction is a target width, the target width is not less than 10 microns and not greater than 25 microns, and the long axis length of the light spot is not less than 50 microns and less than 160 microns. For example, the long axis length of the light spot can be 50 microns, 90 microns, 100 microns, 120 microns, 125 microns, 130 microns, 150 microns, 160 microns, etc.

[0029] The flow direction of the sample flow is a second direction, the first direction is perpendicular to the second direction, and the first direction is perpendicular to the emission direction of the emitted light beam, the long axis of the light spot coincides with a straight line in the first direction, and the short axis of the light spot coincides with a straight line in the second direction.

[0030] Specifically, the target width can have a positive correlation with the minimum long axis length, that is, within the above range, the greater the target width, the greater the long axis length of the light spot can be.

[0031] When irradiating a sample flow channel of a certain target width to form a light spot of a certain long axis length, if the light spot is too small, it is easy to miss irradiating part of the sample in the sample flow, which can easily cause the consequences of flow detection errors, or the edge part of the light spot in the long axis direction is easy to irradiate into the sample, and since the light intensity of this part is poor, it is easy to cause the consequences of flow detection errors,

[0032] Therefore, when the target width can be not less than 10 microns and not greater than 25 microns, the long axis length can be not less than 50 microns and not greater than 160 microns, and under the premise that both the target width and the long axis length meet the above conditions, the possibility of the above problem of the light spot being too small can be reduced, thereby improving the accuracy and reliability of flow detection.

[0033] For example, Figure 1 is a structural schematic view from a first perspective, Figure 2 is a structural schematic view from a second perspective, the line of sight direction corresponding to the first perspective is perpendicular to the line of sight direction corresponding to the second perspective, and the line of sight direction corresponding to the second perspective is parallel to the direction of the light beam emitted by the emission module 11. For example, Figure 2 may be Figure 1 a schematic view of the flow detection module 12 observed from left to right.

[0034] As shown in Figure 2 , the light beam emitted by the emission module 11 forms an elliptical light spot A on the flow detection module 12, part of the elliptical light spot A is on the sample flow channel 121, and the sample in the sample flow channel 121 can be analyzed and detected based on the light beam corresponding to the part of the light spot A on the sample flow channel 121.

[0035] The spot size formed by the emission module 11 on the flow detection module 12 is generally controllable, in order to improve the fluctuation of the light intensity corresponding to the spot formed by the emission module 11 on the flow detection module 12, a preset minimum value of the long axis length of the spot formed by the emission module 11 on the flow detection module 12 can be used to configure the sample flow channel 121 with a corresponding target width, that is, the larger the spot formed by the emission module 11 on the flow detection module 12, the larger the target width of the sample flow channel 121, so that the sample flow in the sample flow channel 121 can be irradiated by the part of the spot with relatively stable light intensity as much as possible, and then the corresponding scattered light or fluorescence is generated for detection.

[0036] Based on the above manner, the stability of the scattered light or fluorescence received by the receiving module 13 in the flow detection can be improved as much as possible, and the reliability of the result of the flow detection is improved.

[0037] Unlike the prior art, in the technical solution of the present application, the target width is not less than 10 microns and not more than 25 microns, and the long axis length is not less than 50 microns and not more than 160 microns. Since the target width is the width of the sample flow channel in the flow detection module, and the long axis length is the long axis length of the spot formed by the light beam emitted by the emission module on the sample flow channel, based on the above manner, the part of the spot irradiated on the sample flow is the part with smaller fluctuation amplitude of light intensity in the whole spot, that is, the flow detection device can use the relatively stable part of the spot to detect the sample flow, and the accuracy of the flow detection is improved.

[0038] In an embodiment, the preset minimum value of the proportion of the light intensity of the spot at the edge of the sample flow channel 121 to the maximum light intensity of the spot is the minimum edge light intensity proportion, and the target width is in a negative correlation with the minimum edge light intensity proportion.

[0039] Specifically, as shown in Figure 2 The light intensity at the center of the spot A in the first direction D1 is the largest and the fluctuation is the smallest, and the light intensity at the leftmost end and the rightmost end of the spot A in the first direction D1 is the smallest and the fluctuation is the largest. By determining the light intensity at the leftmost end or the rightmost end of the part of the spot A irradiated on the sample flow channel 121 in the first direction D1, and dividing it by the light intensity at the center of the spot A, the above-mentioned minimum edge light intensity proportion can be obtained. Through the minimum edge light intensity proportion, the proportion of the weakest light intensity to the strongest light intensity of the spot formed on the sample flow channel 121 can be determined, so that the size of the weakest light intensity of the spot formed on the sample flow channel 121 is determined, and the target width is in a negative correlation with the minimum edge light intensity proportion.

[0040] The minimum edge intensity ratio can be a custom value. This value describes the ratio of the weakest light intensity to the strongest light intensity of the light spot formed on the sample flow channel 121. In other words, under the illumination of the same size light spot, the greater the requirement for the minimum edge intensity ratio, the smaller the corresponding target width needs to be so that the light spot illuminating the sample flow channel 121 can meet the preset minimum edge intensity ratio, thereby improving the accuracy of flow cytometry detection.

[0041] For example, such as Figure 2 As shown, the width of the sample flow channel 121 in the first direction D1 is the target width, and the light intensity distribution of the light spot A in the first direction D1 follows a Gaussian distribution law. The light intensity is highest at the center of the light spot A, while the light intensity of the light spot A gradually weakens along the first direction D1 and in the opposite direction of the first direction D1. Therefore, the larger the minimum edge light intensity ratio, the greater the light intensity of the light spot A at the edge of the sample flow channel 121, which makes the light intensity of the other parts of the sample flow channel 121 illuminated by the light spot A also higher, thus making the part of the sample flow channel 121 illuminated by the light spot A more stable.

[0042] In one embodiment, the preset minimum value of the major axis length of the light spot is the minimum major axis length, the target half-width is half of the target width, the target half-width is the arithmetic square root of the first value, the first value is the quotient of the second value divided by -2, the second value is the product of the third value and the fourth value, the third value is the square of half of the minimum major axis length, and the fourth value is the natural logarithm of the minimum edge light intensity ratio. The minimum edge light intensity ratio is the preset minimum value of the ratio of the light intensity of the light spot at the edge of the sample flow channel 121 to the maximum light intensity of the light spot.

[0043] Based on the above method, the major axis length and minor axis length of the light spot formed on the sample flow channel of the target width can be made more suitable, which improves the light intensity stability of the light illuminating the sample, thereby improving the accuracy of flow cytometry detection.

[0044] Optionally, the minimum edge light intensity ratio is 90%-98%; preferably, the minimum edge light intensity ratio is 92%-98%, for example, the minimum edge light intensity can be 92%, 94%, 95%, 96%, etc., and is not limited thereto.

[0045] Specifically, such as Figure 2 As shown, the light intensity distribution of spot A in the first direction D1 follows a Gaussian distribution law. When the coefficient of variation (CV) of the light intensity at a certain position in the entire region of spot A is between 3% and 5%, it can be considered that the light beam fluctuation at that position is still relatively small. If the CV of the light intensity at a certain position is too large, it can be considered that the light beam fluctuation at that position is relatively large. If sample detection is performed based on light with large fluctuations, the detection accuracy will be easily reduced.

[0046] For example, according to the normal distribution statistical law, the fluctuation range of the light intensity at a position is required to be within ±2SD, SD is the standard deviation, that is, when the CV is 3%, the fluctuation range of the light intensity is ±2*3%, that is, ±6%, which corresponds to the intensity proportion of the Gaussian beam of 94%, and when the CV is 5%, the fluctuation range of the light intensity is ±2*5%, that is, ±10%, which corresponds to the intensity proportion of the Gaussian beam of 90%, and the intermediate value of the two is 92%, which is recorded as the minimum edge light intensity proportion, so that the fluctuation of the light beam irradiated on the edge of the sample flow channel 121 is smaller and the stability is higher. It should be noted that the above is only an example, if the corresponding CV range is changed and it is considered that the fluctuation of the light beam is still small, then other values of the minimum edge light intensity proportion can be obtained, for example, any value between 92%-98% can be obtained, which will not be described here.

[0047] As shown in Figure 3 and 4 , the light intensity distribution formula of the light spot A meeting the above Gaussian distribution law in the first direction D1 is as follows:

[0048]

[0049] wherein, I is the light intensity proportion, Y is the target half-width, ω is half of the minimum major axis length.

[0050] Formula (1) can be converted to the following formula:

[0051]

[0052] Based on formula (2), the appropriate minimum major axis length value range can be obtained, so that the sample flow channel 121 can make the fluctuation of the light beam irradiating the sample flow under the condition of the target width and the minimum major axis length value range smaller, and improve the accuracy of flow detection.

[0053] For example, referring to Figure 3 , Figure 3 is one of the light intensity proportion distribution diagrams of the light spot in the first direction of the present application, as shown in Figure 3 , when the required minimum edge light intensity proportion is any value between 92%-98%, the minimum edge light intensity proportion can be substituted into the light intensity proportion, and if the target width is 10 microns, 5 microns can be substituted into the above Y , the minimum major axis length is greater than or equal to 50 microns.

[0054] Similarly, referring to Figure 4 , Figure 4 is the second light intensity proportion distribution diagram of the light spot in the first direction of the present application, as shown in Figure 4As shown, assuming the required minimum edge light intensity ratio is 92%, the minimum edge light intensity ratio can be substituted into the light intensity ratio, and if the target width is 25 microns, 12.5 microns can be substituted into the above Y In this way, the minimum major axis length is obtained as 126 microns.

[0055] When the minimum edge light intensity ratio is 92%-98%, the minimum value of the minimum edge light intensity ratio is higher than when the minimum edge light intensity ratio is 90%-98%, which can make the minimum light intensity of each part of the sample flow channel 121 irradiated by the light spot A higher, thereby improving the stability of the part of the sample flow channel 121 irradiated by the light spot A, and further improving the accuracy of flow detection.

[0056] Preferably, the minimum major axis length is greater than or equal to 90 microns, that is, when the target width is in the range of 10-25 microns, the minimum major axis length is in the range of 90-126 microns.

[0057] When the required minimum edge light intensity ratio is 92%-98%, the corresponding minimum major axis length range can be obtained by substituting the corresponding value into the light intensity ratio, to adapt to the sample flow channel with a target width in the range of 10-25 microns.

[0058] Based on the above method, a suitable value of the target width can be determined, so that the flow detection accuracy of the flow detection device with the combination of the value range of the target width and the minimum major axis length is improved.

[0059] In addition, the major axis length of the light spot can be not less than 90 microns and less than 160 microns.

[0060] In practice, the quality factor of the quality of the light beam emitted by the laser is not equal to 1, that is, the light intensity distribution of the light spot formed by the laser beam will be different from the theoretical Gaussian distribution, and due to the fluctuation of the sample flow itself, the difference between the light intensity distribution and the theoretical Gaussian distribution is further increased. By making the major axis length of the light spot in the range of not less than 90 microns and less than 160 microns, the laser beam emitted by the laser when forming the light spot is relatively stable, and the possibility of the light spot intensity being unstable due to the influence of light spot offset, sample flow fluctuation, optical power fluctuation, etc. is reduced, thereby improving the accuracy of flow detection.

[0061] In an embodiment, the emission module 11 includes a semiconductor laser, and the wavelength of the light beam emitted by the semiconductor laser ranges from 505 nanometers to 535 nanometers.

[0062] Specifically, the semiconductor laser can be used to emit a light beam to the sample flow channel 121, and the light beam forms a light spot on the sample flow channel 121, and the long axis of the complete elliptical light spot formed on the flow detection module is not less than 90 microns and less than 160 microns, and the short axis is greater than 9 microns and less than 18 microns.

[0063] The wavelength range of the light beam emitted by the semiconductor laser can be 505-535 nanometers, 520-530 nanometers, 515-535 nanometers, or 505-525 nanometers, which is not limited here.

[0064] Based on the above method, the light beam emitted by the semiconductor laser in the above wavelength range is used for sample detection, that is, the semiconductor laser emits a light beam with a wavelength of 505-535 nanometers for flow detection, which can improve the stability of the light spot energy and the accuracy of sample detection compared with the solid laser with a wavelength of 532 nanometers.

[0065] Preferably, the power range of the semiconductor laser is 30-80 milliwatts when the wavelength range of the light beam emitted by the semiconductor laser is 505-535 nanometers.

[0066] Based on the above method, the light beam emitted by the semiconductor laser in the above wavelength range and power range is used for sample detection, that is, the semiconductor laser emits a light beam with a wavelength of 505-535 nanometers and a power of 30-80 milliwatts for flow detection, which can avoid the problem of power jump of the laser during the emission of the light beam, improve the stability of the light spot energy, and further improve the accuracy of sample detection compared with the solid laser with a wavelength of 532 nanometers.

[0067] In an embodiment, when the target width is greater than 10 microns and less than 25 microns, the minimum value of the long axis length is not less than 90 microns and not greater than 126 microns, and the maximum value of the long axis length is less than 160 microns; when the target width is 10 microns, the long axis length is not less than 90 microns and less than 160 microns; when the target width is 25 microns, the long axis length is not less than 126 microns and less than 160 microns.

[0068] For example, when the target width is greater than 10 microns and less than 25 microns, the long axis length can be 90 microns, 95 microns, 100 microns, 110 microns, 120 microns, 130 microns, 140 microns, 150 microns, 160 microns, etc. without limitation; when the target width is 10 microns, the long axis length can be 90 microns, 100 microns, 105 microns, 120 microns, 140 microns, 160 microns, etc. without limitation; when the target width is 25 microns, the long axis length can be 126 microns, 130 microns, 135 microns, 145 microns, 150 microns, 160 microns, etc. without limitation.

[0069] Specifically, in the above combination of the value range of the target width and the long axis length, that is, in the case where the target width is greater than 10 microns and less than 25 microns, the minimum value of the long axis length is not less than 90 microns and not greater than 126 microns, and the maximum value of the long axis length is less than 160 microns, for example, in the case where the target width is 15 microns, the long axis length is not less than 100 microns and less than 160 microns, in the case where the target width is 12 microns, the minimum value of the long axis length is 95, in the case where the target width is 20 microns, the minimum value of the long axis length is 115, etc.; or in the case where the target width is 10 microns and the long axis length is not less than 90 microns and less than 160 microns, or in the case where the target width is 25 microns and the long axis length is not less than 126 microns and less than 160 microns, the stability of the light beam corresponding to the part of the light spot irradiated on the sample flow in the light spot emitted by the emission module 11 can reach a high level, the possibility of the flow detection result being incorrect due to the instability of the intensity of the light beam irradiated on the sample flow is reduced, and the reliability of the flow detection result is improved.

[0070] In an embodiment, the short axis length of the light spot is greater than 9 microns and less than 18 microns. For example, the short axis length of the light spot can be 10 microns, 12 microns, 15 microns, 17 microns, etc. without limitation.

[0071] Specifically, using a large light spot laser to generate a large light spot can easily make the hardware cost of the large light spot laser too high, and using a small light spot laser to generate a large light spot by increasing the power can easily make the overall light intensity of the light spot fluctuate greatly.

[0072] Therefore, by setting the maximum value of the long axis length of the light spot A to 155 microns or 150 microns or other length less than 160 microns, the light intensity stability of the light spot is improved, and the accuracy of the flow detection is further improved.

[0073] For example, based on the constraints of the above conditions, when the target width is 10 microns, the long axis length can be between 90-160 microns, and when the target width is 25 microns, the long axis length can be between 126-160 microns, thereby improving the light intensity stability of the light beam irradiated on the sample in the flow detection device, and further improving the accuracy of flow detection.

[0074] In addition, by making the short axis length of the light spot greater than 9 microns and less than 18 microns, and the long axis length not less than 90 microns and less than 160 microns, the stability of the light spot can be better, and the possibility of light intensity instability caused by light spot deviation, sample flow fluctuation or light power intensity is reduced, thereby improving the accuracy of flow detection.

[0075] In an embodiment, the receiving module 13 includes a silicon photomultiplier.

[0076] The filter wavelength range of the filter configured by the silicon photomultiplier is 555 nanometers to 615 nanometers, and the peak wavelength range of the silicon photomultiplier is 420 nanometers to 650 nanometers.

[0077] And / or, the area of the photosensitive region of the silicon photomultiplier is greater than or equal to 9 square millimeters.

[0078] And / or, the shape of the photosensitive region of the silicon photomultiplier for receiving fluorescence is a square, and the length and width of the square are both 3 millimeters.

[0079] Specifically, the receiving module 13 can be a sensor based on a silicon photomultiplier, the peak wavelength of the light beam that the sensor can receive is in the range of 420-650 nanometers, and the filter wavelength of the filter configured by the sensor is in the range of 555-615 nanometers.

[0080] The peak wavelength range of the sensor based on the silicon photomultiplier can be 420 nanometers to 560 nanometers, 430 nanometers to 640 nanometers, 440 nanometers to 600 nanometers, 550 nanometers to 650 nanometers, and any one of the value ranges between 420-650 nanometers.

[0081] Preferably, by making the filter wavelength range of the filter configured by the silicon photomultiplier be 555 nanometers to 615 nanometers, and the peak wavelength range of the silicon photomultiplier be 420 nanometers to 650 nanometers, the characteristic range of the light beam received by the silicon photomultiplier can be limited respectively, and it should be noted that the signal-to-noise ratio of the silicon photomultiplier when in use is large, so by limiting the bandwidth / wavelength range of the light beam received by the silicon photomultiplier in the above manner, the accuracy of sample detection based on the silicon photomultiplier can be improved.

[0082] In addition, the receiving module 13 can include a filter and a photoelectric sensor, and the light beam filtered by the filter can enter the photoelectric sensor to perform sample detection analysis. The photoelectric sensor can be a silicon photomultiplier as described above.

[0083] The center wavelength in the filtering wavelength range of the filter can correspond to or be the same as the middle value of the peak wavelength range of the photoelectric sensor, so as to improve the fluorescence that can be received by the receiving module 13, improve the utilization rate of the fluorescence, and improve the accuracy of flow detection based on the received fluorescence.

[0084] The photosensitive area of the silicon photomultiplier can be any one of 2.5*2.8 mm, 2.8*2.8 mm, and other sizes. Preferably, by making the area of the photosensitive area of the silicon photomultiplier greater than or equal to 9 mm2or making the shape of the photosensitive area of the silicon photomultiplier be a square of 3*3 mm, it can be ensured that the area of the receiving module 13 for receiving the light beam is large enough, avoiding the situation that part of the light beam is missed and causing sample detection errors, and improving the accuracy of sample detection.

[0085] In an embodiment, the flow detection device further includes a lens group (not shown in the figure), and the lens group includes at least one lens.

[0086] The lens group is located on the light path of the scattered light or the fluorescence, and the lens group is located between the flow detection module 12 and the receiving module 13.

[0087] Specifically, the distance between the lens group and the flow detection module 12 is not less than 0.5 mm and not greater than 1.5 mm, for example, which can be 0.5 mm, 0.8 mm, 1 mm, 1.2 mm, 1.3 mm, and the like, without limitation; it can avoid the situation that the overall volume of the flow detection device is too large or the light intensity of the light received by the receiving module 13 is too small, and it can balance the device volume and the optical detection effect, adapt to different sizes of sample analysis instruments, and improve the applicability and flexibility of the lens group in application.

[0088] The lens group can also be configured with a displacement module for controlling the lens group to translate in the first direction and / or the second direction.

[0089] In an embodiment, the flow detection device is applied to an immune analyzer, and the flow detection module 12 is a flow detection module that detects by using a magnetic bead method.

[0090] The short axis length of the light spot is greater than the diameter of one magnetic bead and less than the diameter of two magnetic beads.

[0091] Specifically, the short axis length of the light spot needs to be in the range of 9-18 microns while meeting the power requirement of the light spot area of the emitting module 11.

[0092] Based on the above manner, the situation that a single magnetic bead is not completely irradiated in a single detection or both magnetic beads are irradiated in a single detection can be avoided due to the too long or too short short axis, and the accuracy of flow detection is improved.

[0093] In addition, it should be noted that the power requirement can be specifically the requirement of the spot power density, and the spot power density can be specifically a value obtained by dividing the light power of a light spot corresponding to a light beam by the spot area. The requirement of the spot power density can be specifically that when the spot power density of the sample detection required spot is within 9-18 microns, the long axis length of the spot needs to be less than 160 microns, so as to ensure that the spot power density of the spot can meet the requirements of sample detection, ensure that the energy of each part of the spot can meet the detection requirements, and further ensure the reliability of sample detection.

[0094] The diameter of the magnetic bead is associated with the type of magnetic bead detection, and different types of magnetic bead detection projects can use magnetic beads of different sizes. Correspondingly, the value range of the short axis length of the spot is also determined by the corresponding size of the magnetic bead.

[0095] In an example, the sample detection performed by the flow detection device can be immunomagnetic bead detection. In immunomagnetic bead detection, antigens or antibodies of different test substances can be covalently cross-linked to specific fluorescently encoded magnetic beads to form a combination of “magnetic bead-test substance-fluorescent marker”. By making the combination pass through the sample flow channel 121 one by one for light beam irradiation and fluorescence generation, corresponding immunomagnetic bead detection can be performed based on the generated fluorescence.

[0096] In an embodiment, the flow detection device can also be applied to a blood analyzer, and the flow detection module 12 is specifically an optical detection module that performs detection by flow cytometry.

[0097] The short axis length of the spot is greater than the diameter of one cell and less than the diameter of two cells.

[0098] Specifically, the short axis length of the spot needs to be within the range of 9-18 microns while the spot area meets the power requirement of the emission module 11.

[0099] Based on the above manner, the situation that a single magnetic bead is not completely irradiated in a single detection or both magnetic beads are irradiated in a single detection can be avoided due to the too long or too short short axis, and the accuracy of flow detection is improved.

[0100] Further, it should be noted that the power requirement can be specifically a requirement for the spot power density, the spot power density can be specifically a value obtained by dividing the light power of a light spot corresponding to a light beam by the spot area, and the requirement for the spot power density can be specifically that, when the spot power density of the light spot required for sample detection and the short axis of the light spot is within 9-18 microns, the long axis length of the light spot is less than 160 microns, so as to ensure that the spot power density of the light spot can meet the requirement for sample detection, ensure that the energy of each part of the light spot can meet the detection requirement, and further ensure the reliability of sample detection.

[0101] The present application also provides a sample analyzer, which can be seen from Figure 5 , Figure 5 is a structural schematic diagram of an embodiment of the sample analyzer of the present application, as Figure 5 shown, the sample analyzer 20 comprises a flow detection device 21, which can be the flow detection device described in any one of the foregoing embodiments, and details are not repeated here.

[0102] In an embodiment, the sample analyzer 20 can be an immune analyzer, which further comprises a sample adding assembly, a reaction assembly and a sampling module.

[0103] The sample adding assembly is used for injecting the sample and reagent into the reaction cup.

[0104] The reaction assembly is used for reacting the sample and the reagent in the reaction cup, wherein the reagent comprises a plurality of detection microspheres, and various types of detection microspheres correspond to corresponding detection items respectively.

[0105] The sampling module is used for collecting the sample to the flow detection device 21, so as to obtain the detection result corresponding to each detection item by flow detection.

[0106] Specifically, the sampling module can be specifically used for transporting the reaction liquid generated in the reaction cup to the flow detection device 21, and the flow detection device 21 can form a corresponding sample flow by using the reaction liquid to perform flow detection.

[0107] The sample analyzer 20 can also be other types of sample analyzers, which are not limited here.

[0108] Differing from the prior art, in the technical scheme of the present application, the target width is not less than 10 microns and not more than 25 microns, and the long axis length is not less than 50 microns and not more than 160 microns. Since the target width is the width of the sample flow channel in the flow detection module, and the long axis length is the long axis length of the light spot formed by the light beam emitted by the emission module on the sample flow channel, based on the above-mentioned manner, the part of the light spot irradiated on the sample flow is the part with smaller light intensity fluctuation amplitude in the whole light spot, that is, the flow detection device can use the relatively stable part of the light spot to detect the sample flow, thereby improving the accuracy of flow detection.

[0109] The above only describes the embodiments of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation based on the content of the specification and drawings, or direct or indirect application in other related technical fields, is also included in the patent protection scope of the present application.

Claims

1. A flow detection device, characterized by The flow detection device comprises a transmitting module, a flow detection module and a receiving module, and the flow detection module comprises a sample flow channel; The sample flow channel is used to transport a sample flow to be detected, the transmitting module is used to emit a light beam to the sample flow channel to form a light spot on the sample flow channel, and the receiving module is used to receive scattered light scattered by the emitted light beam on the sample flow or fluorescence formed by exciting the sample to perform sample detection analysis; The width of the sample flow channel in a first direction is a target width, the target width is not less than 10 microns and not greater than 25 microns, and the long axis length of the light spot is not less than 50 microns and less than 160 microns; The flow direction of the sample flow is a second direction, the first direction is perpendicular to the second direction, and the first direction is perpendicular to the emission direction of the light beam, the long axis of the light spot coincides with a straight line in the first direction, and the short axis of the light spot coincides with a straight line in the second direction; The preset minimum value of the proportion of the light intensity of the light spot at the edge of the sample flow channel to the maximum light intensity of the light spot is a minimum edge light intensity proportion, and the target width and the minimum edge light intensity proportion are in a negative correlation relationship; The preset minimum value of the long axis length of the light spot is a minimum long axis length, the target half width is half of the target width, the target half width is the arithmetic square root of a first value, the first value is the quotient of a second value divided by -2, the second value is the product of a third value and a fourth value, the third value is the square of half of the minimum long axis length, and the fourth value is the natural logarithm of the minimum edge light intensity proportion.

2. The flow detection device of claim 1, wherein, The minimum edge light intensity proportion is between 90% and 98%.

3. The flow detection device of claim 1, wherein, The transmitting module comprises a semiconductor laser, and the wavelength range of the light beam emitted by the semiconductor laser is 505 nanometers to 535 nanometers.

4. The flow detection device of claim 1, wherein, The receiving module comprises a silicon photomultiplier; The filter wavelength range of the filter configured for the silicon photomultiplier is 555 nanometers to 615 nanometers, and the peak wavelength range of the silicon photomultiplier is 420 nanometers to 650 nanometers.

5. The flow detection device of claim 1, wherein, The flow detection device further comprises a lens group, and the lens group comprises at least one lens; The lens group is located on the light path of the fluorescence, and the lens group is located between the flow detection module and the receiving module; The distance between the lens group and the flow detection module is not less than 0.5 millimeters and not greater than 1.5 millimeters.

6. The flow detection device of claim 1, wherein, The short axis length of the light spot is greater than 9 microns and less than 18 microns; When the target width is greater than 10 microns and less than 25 microns, the minimum value of the long axis length is not less than 90 microns and not greater than 126 microns, and the maximum value of the long axis length is less than 160 microns; When the target width is 10 microns, the long axis length is not less than 90 microns and less than 160 microns; When the target width is 25 microns, the long axis length is not less than 126 microns and less than 160 microns.

7. A sample analyzer characterized by, The flow detection device comprises the flow detection device according to any one of claims 1 to 6.

8. The sample analyzer of claim 7, wherein, The sample analyzer is an immune analyzer, and the immune analyzer further comprises A sample adding assembly is configured to inject a sample and reagents into a reaction cup; A reaction assembly is configured to react the sample in the reaction cup with the reagents; wherein the reagents include a plurality of detection microspheres, each type of the detection microspheres corresponding to a corresponding detection item; A sampling module is configured to collect the sample to the flow detection device, so as to obtain a detection result corresponding to each detection item by the flow detection device.

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