Sequencing chips and sequencing systems

Abstract

The present application relates to a sequencing chip and a sequencing system. The sequencing chip includes: a gene cluster; a chip body, wherein a cavity is formed in the chip body; and an adjustment plate, wherein the adjustment plate is connected to the chip body and can separate the cavity into multiple test channels arranged along the thickness direction of the chip body. The gene cluster is arranged in the test channels, and any two adjacent test channels are not connected to each other. When the fluorescence generated by the gene cluster excitation passes through the adjustment plate, the adjustment plate focuses the fluorescence to a specified position. Due to the presence of the adjustment plate, the fluorescence generated by the gene cluster excitation in the multiple test channels can be concentrated at the theoretical focus of the objective lens, thereby avoiding the optical device being unable to clearly image the gene cluster located outside the depth of field due to the limitation of the depth of field of the objective lens, thereby improving the reliability of gene sequencing while reducing manufacturing costs.

Description

Technical Field

[0001] The present application relates to the field of gene sequencing technology, and in particular to a sequencing chip and a sequencing system comprising the sequencing chip. Background Art

[0002] With the continuous advancement of science and technology, human genetic research is also deepening. Gene sequencing, as a key technical means, can help humans understand the structure and function of the genome, thus having important implications for the study of human health and disease. During the gene sequencing process, an optical device captures the gene clusters within a sequencing chip to generate a sequencing image. However, traditional sequencing chips with multiple test channels often result in high manufacturing costs and low reliability for sequencing systems using these chips. Summary of the Invention

[0003] A technical problem solved by the present application is how to improve the reliability of a sequencing system using the sequencing chip while reducing manufacturing costs.

[0004] A sequencing chip, applied to a sequencing system, comprising:

[0005] include:

[0006] gene clusters;

[0007] a chip body, wherein a cavity is defined in the chip body; and

[0008] An adjusting plate is connected to the chip body, and the adjusting plate can separate the cavity to form a plurality of test flow channels arranged along the thickness direction of the chip body. The gene cluster is arranged in the test flow channels, and any two adjacent test flow channels are not connected to each other. When the fluorescence generated by the excitation of the gene cluster passes through the adjusting plate, the adjusting plate focuses the fluorescence to a specified position.

[0009] In one embodiment, the gene cluster is fixed on the regulatory sheet or the chip body.

[0010] In one embodiment, two test channels that are adjacent in test time are respectively recorded as a front test channel and a rear test channel. When sequencing is performed on the gene cluster in the front test channel, a biochemical reaction is performed on the gene cluster in the rear test channel.

[0011] In one embodiment, the adjustment sheet includes an adjustment portion located in the cavity, the adjustment portion has two adjustment surfaces in the thickness direction, and the adjustment sheet further includes a microstructure, and the microstructure is provided on at least one of the two adjustment surfaces.

[0012] In one embodiment, the microstructure is a protrusion or a groove.

[0013] In one embodiment, the chip body includes a first chip and a second chip, the first chip is provided with a first groove, the second chip is provided with a second groove, the adjustment plate is connected between the first chip and the second chip, and the adjustment plate separates the first groove and the second groove to form two test flow channels that are not connected to each other.

[0014] In one embodiment, the first chip is made of a transparent material, the second chip is made of a transparent or non-transparent material, the thickness of the first chip is less than or equal to the thickness of the second chip, and the depth of the first groove is greater than or equal to the depth of the second groove.

[0015] In one embodiment, the first chip is made of glass material, and the second chip is made of glass or silicone material.

[0016] In one embodiment, at least one of the following options is also included:

[0017] For the gene clusters in two adjacent test flow channels, along the thickness direction of the chip body, the orthographic projection of the gene cluster in one test flow channel can cover the gene cluster in the other test flow channel;

[0018] The thickness of the chip body is in micron order.

[0019] A sequencing system comprises an optical device, a mobile platform and a sequencing chip as described above, wherein the sequencing chip is carried on the mobile platform, and the optical device is used to sequence the gene cluster.

[0020] A technical effect of one embodiment of the present application is that, due to the presence of the adjustment plate, the fluorescence generated by the excitation of the gene clusters in multiple test flow channels can be concentrated at the theoretical focus of the objective lens, thus preventing the optical device from being unable to clearly image the gene clusters located outside the depth of field due to the limitations of the objective lens depth of field, thereby ensuring that the optical device can clearly image the gene clusters in each test flow channel, ultimately improving the test accuracy of gene sequencing. At the same time, the adjustment plate has a simple structure, which can enable the sequencing system using this sequencing chip to avoid the use of a more expensive compensation mechanism, thereby reducing the manufacturing cost of the sequencing system and thus reducing the testing cost of gene sequencing. The adjustment plate is also statically set and does not need to move, thereby avoiding malfunctions caused by the movement process, ensuring that the test is carried out smoothly, and improving the reliability of the sequencing system using this sequencing chip during the test process, that is, improving the reliability of gene sequencing. Therefore, by using this sequencing chip to sequence genes, the sequencing system using this sequencing chip can be improved in reliability on the basis of reducing manufacturing costs, that is, reducing the cost of gene sequencing and improving the reliability of gene sequencing. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A schematic diagram of the planar structure of a sequencing system provided in one embodiment.

[0022] Figure 2 A schematic diagram of the planar structure of a sequencing system provided in another embodiment.

[0023] Figure 3 A schematic diagram of the planar structure of a sequencing chip provided in one embodiment.

[0024] Figure 4 A schematic diagram of the planar structure of a sequencing chip provided in yet another embodiment.

[0025] Figure 5 A schematic diagram of the planar structure of a sequencing chip provided in yet another embodiment.

[0026] Figure 6 A schematic diagram of the planar structure of a sequencing chip provided in yet another embodiment.

[0027] Figure 7 A schematic diagram of the planar structure of a sequencing chip provided in yet another embodiment.

[0028] Figure 8 A schematic diagram of a planar structure in which a first gene cluster in a sequencing chip is tested by an optical device is provided as an embodiment.

[0029] Figure 9 A schematic diagram of a planar structure in which a second gene cluster in a sequencing chip is tested by an optical device is provided as an embodiment.

[0030] Figure 10 for Figure 9Schematic diagram of the local enlarged structure.

[0031] Reference numerals: optical device 10, mobile platform 20, sequencing chip 30, sequencing system 40, excitation light source 110, collimating lens group 120, excitation filter 130, dichroic mirror 140, first dichroic mirror 141, second dichroic mirror 142, objective lens 150, emission filter 160, first emission filter 161, second emission filter 162, tubular lens 170, first tubular lens 171, second tubular lens 172, camera 180 , first camera 181, second camera 182, reflector 190, gene cluster 200, first gene cluster 210, second gene cluster 220, chip body 300, first chip 310, first groove 311, second chip 320, second groove 321, cavity 330, test channel 340, first test channel 341, second test channel 342, adjustment plate 400, adjustment part 410, adjustment surface 411, protrusion 420. DETAILED DESCRIPTION

[0032] To make the above-mentioned objects, features, and advantages of the present application more clearly understood, the specific embodiments of the present application are described in detail below with reference to the accompanying drawings. The following description sets forth many specific details to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways than those described herein, and those skilled in the art can make similar improvements without violating the scope of the present application. Therefore, the present application is not limited to the specific embodiments disclosed below.

[0033] In the description of this application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0034] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, if the term "plurality" appears, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.

[0035] In this application, unless otherwise specified or limited, the terms "mounted," "connected," "connected," "fixed," etc., should be interpreted broadly. For example, these terms may refer to fixed connections, removable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediary; and internal communication between two components or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0036] In this application, unless otherwise expressly specified or limited, if a first feature is described as being "above" or "below" a second feature, or similar descriptions, this may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is described as being "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is described as being "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0037] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only embodiment.

[0038] See Figure 1 In one embodiment of the present application, a sequencing system 40 is provided for sequencing genes. The sequencing system 40 includes an optical device 10, a mobile platform 20, and a sequencing chip 30. The sequencing chip 30 can be carried on the mobile platform 20, which can move linearly. The optical device 10 is used to sequence gene clusters 200 on the sequencing chip 30. As the sequencing chip 30 moves with the mobile platform 20, the optical device 10 can sequence each gene cluster 200 on the sequencing chip 30 in a sequential manner.

[0039] See Figure 1In some embodiments, for example, the optical device 10 may include an excitation light source 110, a collimating lens group 120, an excitation filter 130, a dichroic mirror 140, an objective lens 150, an emission filter 160, a tube lens 170, and a camera 180. Excitation light emitted by the excitation light source 110 is emitted as a parallel beam through the collimating lens group 120. The parallel beam passes through the excitation filter 130 and strikes the dichroic mirror 140. The parallel beam is then reflected by the dichroic mirror 140 and enters the objective lens 150, where it is finally focused onto the gene cluster 200 on the sequencing chip 30. Because gene cluster 200 is labeled with a fluorescent dye, it generates fluorescence when irradiated with excitation light. The fluorescence passes sequentially through objective lens 150, dichroic mirror 140, and emission filter 160 to illuminate tube lens 170. Tube lens 170 focuses the fluorescence onto camera 180, which then collects and converts the acquired fluorescence to generate a sequencing image, completing the sequencing of gene cluster 200. Excitation light source 110 can be a laser, LED, halogen lamp, or other light source.

[0040] See Figure 2 For example, the optical device 10 may include an excitation light source 110, a collimating lens group 120, an excitation filter 130, a first dichroic mirror 141, an objective lens 150, a second dichroic mirror 142, a reflecting mirror 190, a first emission filter 161, a second emission filter 162, a first tube lens 171, a second tube lens 172, a first camera 181 and a second camera 182. Excitation light from the excitation light source 110 passes through the collimating lens assembly 120 as a parallel beam, passing through the excitation filter 130 and irradiating the first dichroic mirror 141. This light is then reflected by the first dichroic mirror 141 and directed to the objective lens 150, where it is focused onto the gene cluster 200 on the sequencing chip 30. Because the gene cluster 200 is labeled with a fluorescent dye, the gene cluster 200 generates fluorescence under the excitation light. This fluorescence passes through the objective lens 150 and the first dichroic mirror 141, and then onto the second dichroic mirror 142. The fluorescence is then divided into a first fluorescence wavelength band and a second fluorescence wavelength band. The first fluorescence wavelength band passes through the second dichroic mirror 142 and the first emission filter 161, then onto the first cylindrical lens 171. This wavelength band is then focused by the first cylindrical lens 171 onto the first camera 181, which collects and converts the captured fluorescence to generate a sequencing image, completing the sequencing of the gene cluster 200. The second fluorescence band is reflected by the second dichroic mirror 142 and the reflector 190 and passes through the second emission filter 162 to illuminate the second tube lens 172, and is focused onto the second camera 182 under the action of the second tube lens 172. The second camera 182 collects and converts the acquired fluorescence to generate a sequencing image to complete the sequencing of the gene cluster 200.

[0041] See Figure 3In some embodiments, the sequencing chip 30 includes a gene cluster 200, a chip body 300, and an adjustment sheet 400. A cavity 330 is defined within the chip body 300, and the adjustment sheet 400 is connected to the chip body 300. The number of adjustment sheets 400 can be one or more, and all adjustment sheets 400 can separate the cavity 330 to form multiple test flow channels 340. The multiple test flow channels 340 are arranged along the thickness direction of the chip body 300, and any two adjacent test flow channels 340 are not connected to each other. The gene cluster 200 is disposed within the test flow channels 340, for example, there can be two or more test flow channels 340. When the fluorescence generated by the gene cluster 200 is excited and passes through the adjustment sheet 400, the adjustment sheet 400 focuses the fluorescence to a specified position, for example, the fluorescence can be focused to the focal position of the objective lens 150.

[0042] See Figure 3 In some embodiments, the chip body 300 includes a first chip 310 and a second chip 320. The first chip 310 can be made of a transparent material so that fluorescence and excitation light can pass through the first chip 310. For example, the first chip 310 can be made of glass. The second chip 320 can be made of a transparent or non-transparent material. For example, the second chip 320 can be made of glass or silicone. A first groove 311 is formed on the first chip 310, and the first groove 311 can be formed by an etching process. A second groove 321 is formed on the second chip 320, and the second groove 321 can also be formed by an etching process. The regulating plate 400 is connected between the first chip 310 and the second chip 320. The first groove 311 and the second groove 321 can be understood as the cavity 330 of the chip body 300. The regulating plate 400 separates the first groove 311 and the second groove 321 from each other, thereby forming two independent test channels 340 that are not connected to each other. The two test channels 340 are respectively denoted as the first test channel 341 and the second test channel 342. The first test channel 341 is formed by the first groove 311, and the second test channel 342 is formed by the second groove 321. Gene clusters 200 are respectively disposed in the first test channel 341 and the second test channel 342. The gene cluster 200 disposed in the first test channel 341 is denoted as the first gene cluster 210, and the gene cluster 200 disposed in the second test channel 342 is denoted as the second gene cluster 220.

[0043] See Figure 4 、 Figure 5 、 Figure 6 and Figure 7The gene cluster 200 is fixed to the regulating plate 400 or the chip body 300. The first gene cluster 210 can be fixed to the bottom wall of the first groove 311, that is, the first gene cluster 210 is fixed to the first chip 310. The first gene cluster 210 can also be fixed to the regulating plate 400, that is, the first gene cluster 210 is fixed to the surface of the regulating plate 400 that defines the first test flow channel 341, and this surface is spaced apart from the bottom wall of the first groove 311 along the thickness direction of the chip body 300. The second gene cluster 220 can be fixed to the bottom wall of the second groove 321, that is, the second gene cluster 220 is fixed to the second chip 320. The second gene cluster 220 can also be fixed to the regulating plate 400, that is, the second gene cluster 220 is fixed to the surface of the regulating plate 400 that defines the second test flow channel 342, and this surface is spaced apart from the bottom wall of the second groove 321 along the thickness direction of the chip body 300.

[0044] See Figure 3 In some embodiments, for gene clusters 200 within two adjacent test channels 340, along the thickness direction of the chip body 300, the orthographic projection of the gene cluster 200 within one test channel 340 can cover the gene cluster 200 within the other test channel 340. For example, when there are two test channels 340, the orthographic projection of the first gene cluster 210 within the first test channel 341 can cover at least a portion of the second gene cluster 220 within the second test channel 342. In short, the orthographic projection of the first gene cluster 210 covers at least a portion of the second gene cluster 220.

[0045] In some embodiments, the thickness of the first chip 310 is less than or equal to the thickness of the second chip 320, the depth of the first groove 311 is greater than or equal to the depth of the second groove 321, and the thickness of the chip body 300 can be in the micron range. For example, the thickness of the first chip 310 can be approximately 250 μm, and the depth of the first groove 311 can be approximately 100 μm. The thickness of the second chip 320 can be approximately 700 μm, and the depth of the second groove 321 can be approximately 70 μm.

[0046] See Figure 3 、 Figure 4 and Figure 5In some embodiments, the adjustment sheet 400 includes an adjustment portion 410 located within the cavity 330, and the adjustment portion 410 has two adjustment surfaces 411 in the thickness direction. The adjustment sheet 400 may also include a micron-scale microstructure, and at least one of the two adjustment surfaces 411 may be provided with the microstructure. For example, the microstructure may be a raised portion 420, and the number of raised portions 420 may be multiple, and at least one of the two adjustment surfaces 411 may be provided with multiple raised portions 420 at intervals, that is, the raised portions 420 may be provided on at least one of the two adjustment surfaces 411. For another example, the microstructure may be a groove, and at least one of the two adjustment surfaces 411 may be recessed to form multiple grooves, and the grooves may be provided at intervals on the adjustment surface 411, that is, the grooves may be provided on at least one of the two adjustment surfaces 411. For another example, at least one of the two adjustment surfaces 411 may be provided with both grooves and raised portions 420. By providing microstructures such as grooves or raised portions 420 on the adjustment portion 410, the adjustment plate 400 acts as a lens, thereby having a focusing effect on the fluorescence generated by the excitation of the gene cluster 200, thereby focusing the fluorescence to a specified position, which can be the focal position of the objective lens 150. Of course, the microstructure can also be in other forms, as long as it can focus the fluorescence to a specified position. The adjustment plate 400 can be made of a transparent material so that the fluorescence and the excitation light can pass through the adjustment plate 400. For example, the adjustment plate 400 can be made of a glass material. The microstructures such as grooves and raised portions 420 can be formed by processes such as nanoimprinting or etching. Glue can be applied to the adjustment plate 400 so that the adjustment plate 400 can glue the first chip 310 and the second chip 320 together.

[0047] See Figure 8 、 Figure 9 and Figure 10 During the test, the theoretical focus of the objective lens 150 can fall on the first gene cluster 210, so that the optical device 10 can clearly image the first gene cluster 210, thereby ensuring the test accuracy of the first gene cluster 210. When testing the second gene cluster 220, due to the effect of the adjustment plate 400, the fluorescence generated by the second gene cluster 220 can be focused on the theoretical focus of the objective lens 150, so that the optical device 10 can also clearly image the second gene cluster 220, thereby ensuring the test accuracy of the second gene cluster 220. Of course, the theoretical focus of the objective lens 150 can be located on the second gene cluster 220, so that the second gene cluster 220 can be clearly imaged to ensure test accuracy; when it is necessary to test the first gene cluster 210, due to the effect of the adjustment plate 400, the fluorescence generated by the first gene cluster 210 can be focused on the theoretical focus of the objective lens 150, so that the optical device 10 can also clearly image the first gene cluster 210.

[0048] In some embodiments, during the testing process, the gene cluster 200 and the reaction solution must first undergo a sufficient biochemical reaction, and then the biochemically reacted gene cluster 200 can be tested using the optical device 10. Two adjacent test channels 340 at the time of testing are designated as the leading test channel 340 and the trailing test channel 340. While sequencing is being performed on the gene cluster 200 in the leading test channel 340, a biochemical reaction is being performed on the gene cluster 200 in the trailing test channel 340. For example, the first test channel 341 is the front test channel, and the second test channel 342 is the rear test channel. That is, when the first gene cluster 210 in the first test channel 341 is tested by the optical device 10, a biochemical reaction can be performed on the second gene cluster 220 in the second test channel 342. When the reaction of the second gene cluster 220 is completed, the test of the first gene cluster 210 is just completed. In this way, the optical device 10 can test the second gene cluster 220, that is, the tests between the second gene cluster 220 and the first gene cluster 210 can be seamlessly connected.

[0049] If the adjustment plate 400 is not provided between the first chip 310 and the second chip 320, the first groove 311 and the second groove 321 are interconnected due to the absence of the adjustment plate 400. This means that the first test channel 341 and the second test channel 342 lose their independence, effectively forming a single test channel 340. In this case, the first gene cluster 210 is disposed on the bottom wall of the first groove 311, and the second gene cluster 220 is disposed on the bottom wall of the second groove 321. The theoretical focus of the objective lens 150 can fall on the first gene cluster 210.

[0050] During the test, the theoretical focus of the objective lens 150 falls on the first gene cluster 210. Since the maximum depth of field of the objective lens 150 typically does not exceed 1 μm, and the spacing between the first gene cluster 210 and the second gene cluster 220 along the thickness direction of the chip body 300 is greater than 1 μm, the second gene cluster 220 is located outside the depth of field of the objective lens 150, resulting in the camera 180 being unable to clearly image the second gene cluster 220, ultimately affecting the test accuracy of the second gene cluster 220. If a compensation mechanism is provided on the optical device 10 to compensate for the phase difference of the second gene cluster 220, this will increase the manufacturing cost of the optical device 10, thereby increasing the test cost of gene sequencing. Furthermore, a motor will be required to drive the compensation mechanism. Since the motor and compensation structure are prone to failure, this will also reduce the reliability of the optical device 10, thereby reducing the test reliability of gene sequencing. Alternatively, if objective lens 150 has a focusing function, that is, when testing the first gene cluster 210, the objective lens 150 focuses on the first gene cluster 210, and when testing the second gene cluster 220, the focusing function can be used to focus the objective lens 150 on the second gene cluster 220, thus achieving clear imaging. However, the provision of a focusing function will also increase the manufacturing cost of the optical device 10, and thus increase the cost of gene sequencing testing. Furthermore, if objective lens 150 malfunctions and cannot achieve the focusing function, the reliability of the optical device 10 and the reliability of the gene sequencing test will also be reduced.

[0051] The first gene cluster 210 can have already completed its biochemical reaction outside of the test channel 340, so it can be tested directly using the optical device 10. However, the second gene cluster 220 must complete its biochemical reaction within the test channel 340 before it can be tested. Because the first and second test channels 341, 342 are interconnected, effectively forming a single test channel 340, when testing the first gene cluster 210, to prevent the reaction solution from interfering with the testing of the first gene cluster 210, the reaction solution cannot be introduced into the test channel 340 to initiate a biochemical reaction with the second gene cluster 220. This means that only after the testing of the first gene cluster 210 is completed can the reaction solution be introduced into the test channel 340 to initiate a biochemical reaction with the second gene cluster 220, ultimately allowing testing of the second gene cluster 220, which has completed its biochemical reaction. Therefore, after the first gene cluster 210 is tested, the optical device 10 cannot immediately test the second gene cluster 220. It still needs to wait until the second gene cluster 220 completes the biochemical reaction before it can be tested. This causes an intermediate waiting time for the optical device 10, thereby affecting the test efficiency, that is, affecting the test throughput.

[0052] See Figure 8 、 Figure 9 and Figure 10Regarding the sequencing chip 30 in the above-described embodiment, on the one hand, the presence of the adjustment plate 400 allows the fluorescence generated by the gene clusters 200 within each test flow channel 340 to be focused at the theoretical focus of the objective lens 150. This prevents the optical device 10 from being unable to clearly image the gene clusters 200 located outside the depth of field of the objective lens 150 due to the limited depth of field, thereby ensuring that the optical device 10 clearly images the gene clusters 200 within each test flow channel 340, ultimately improving the accuracy of gene sequencing testing. On the other hand, the presence of the adjustment plate 400 provides a simple structure and relatively low manufacturing cost. This avoids the need for a complex compensation mechanism and the provision of an objective lens 150 with a focusing function, thereby reducing the manufacturing cost of the optical device 10 and, in other words, the cost of gene sequencing testing. Furthermore, the adjustment plate 400 is stationary and does not require movement, thereby avoiding malfunctions that may occur during movement, ensuring smooth testing, and improving the reliability of the optical device 10, thereby improving the reliability of gene sequencing. On the other hand, due to the presence of the regulating plate 400, the two adjacent test channels 340 can be prevented from being connected to each other. When the gene cluster 200 in the front test channel 340 is tested, the reaction liquid can be introduced into the rear test channel 340 to prevent the reaction liquid from flowing into the front test channel 340. After the test of the gene cluster 200 in the front test channel 340 is completed, the gene cluster 200 in the rear test channel 340 can just complete the biochemical reaction. Therefore, the optical device 10 can immediately test the gene cluster 200 in the rear test channel 340 that has completed the biochemical reaction, so that the tests of the gene cluster 200 in the rear test channel 340 and the gene cluster 200 in the front test channel 340 can be seamlessly connected, thereby eliminating the intermediate waiting time of the optical device 10, and ultimately improving the test efficiency of gene sequencing, that is, improving the test throughput.

[0053] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0054] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, and these modifications and improvements fall within the scope of protection of the present application. Therefore, the scope of protection of the present patent application shall be determined by the appended claims.

Claims

1. A sequencing chip, used in a sequencing system, characterized in that: The sequencing chip comprises: gene clusters; a chip body, wherein a cavity is defined in the chip body; and an adjustment sheet connected to the chip body, capable of dividing the cavity into a plurality of test flow channels arranged along the thickness direction of the chip body, wherein the gene clusters are disposed in the test flow channels, and any two adjacent test flow channels are not connected to each other, and when the fluorescence generated by the excitation of the gene clusters passes through the adjustment sheet, the adjustment sheet focuses the fluorescence to a designated position; The regulating plate includes an regulating portion located in the cavity, the regulating portion having two regulating surfaces in the thickness direction, and the regulating plate further includes a microstructure, at least one of the two regulating surfaces being provided with the microstructure; the microstructure is a protrusion or a groove.

2. The sequencing chip according to claim 1, wherein The gene cluster is fixed on the regulatory sheet or the chip body.

3. The sequencing chip according to claim 1, wherein Two test channels that are adjacent in test time are respectively recorded as the front test channel and the back test channel. When the gene cluster in the front test channel is sequenced, the gene cluster in the back test channel is subjected to a biochemical reaction.

4. The sequencing chip according to claim 1, wherein The regulating piece is made of transparent material.

5. The sequencing chip according to claim 4, characterized in that The regulating piece is made of glass material.

6. The sequencing chip according to claim 1, wherein The chip body includes a first chip and a second chip, the first chip is provided with a first groove, the second chip is provided with a second groove, the adjustment plate is connected between the first chip and the second chip, and the adjustment plate separates the first groove and the second groove to form two test flow channels that are not connected to each other.

7. The sequencing chip according to claim 6, characterized in that The first chip is made of a transparent material, the second chip is made of a transparent or non-transparent material, the thickness of the first chip is less than or equal to the thickness of the second chip, and the depth of the first groove is greater than or equal to the depth of the second groove.

8. The sequencing chip according to claim 7, characterized in that The first chip is made of glass material, and the second chip is made of glass or silicone material.

9. The sequencing chip according to claim 1, wherein Also includes at least one of the following options: For the gene clusters in two adjacent test flow channels, along the thickness direction of the chip body, the orthographic projection of the gene cluster in one test flow channel can cover the gene cluster in the other test flow channel; The thickness of the chip body is in micron order.

10. A sequencing system, characterized in that: The method comprises an optical device, a mobile platform and a sequencing chip according to any one of claims 1 to 9, wherein the sequencing chip is carried on the mobile platform, and the optical device is used to sequence the gene cluster.

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