A hydrogel dressing for promoting wound healing and preparation method thereof

Abstract

The present invention belongs to the field of medical technology, and in particular to a hydrogel dressing for promoting wound healing and a preparation method thereof. The hydrogel dressing includes the following raw materials in parts by weight: 10-18 parts of gel matrix, 1-5 parts of silk fibroin, 0.3-0.8 parts of scutellariae, 0.1-0.5 parts of diphenylethylene glycosides, 0.4-0.8 parts of modified soybean lecithin, 0.5-1 parts of trehalose, and 80-100 parts of deionized water. The hydroxyl groups in the modified soybean lecithin and the amino groups of the components such as chitosan in the gel matrix form hydrogen bonds, thereby improving the mechanical properties of the hydrogel dressing. In addition, the modified soybean lecithin can also increase the water content of the stratum corneum of the skin, which is beneficial to the repair of scars after wound healing. And the results of wound healing experiments show that the use of scutellariae and diphenylethylene glycosides can effectively promote wound healing.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine, and particularly relates to a hydrogel dressing for promoting wound healing and a preparation method thereof. Background Art

[0002] Skin is the first barrier of the human body against the external environment and microorganisms, and is therefore the most vulnerable organ. Various external stimuli or internal pathologies can lead to skin wounds. Bacterial infection is one of the main reasons for the formation of chronic wounds and difficulty in healing. When a wound is infected, it often leads to a prolonged wound healing cycle or even death. Dressings are the key to effective wound management.

[0003] In the field of wound repair technology, a variety of wound dressings have been developed to support and accelerate the healing process of wounds. Hydrogel dressings are a new type of medical material that has received much attention due to its excellent flexibility, moisture retention, breathability and biocompatibility. More importantly, hydrogel dressings can provide a moist healing environment for wounds, promote cell proliferation at the wound site, and effectively prevent the formation of scars. At the same time, they can also absorb wound exudate and minimize the nutrients required for bacterial growth, reducing the risk of infection.

[0004] Existing hydrogel dressings have technical problems such as poor wound healing ability and easy scarring. Based on this, it is necessary to further explore the performance of hydrogel dressings to better meet market demand. Summary of the invention

[0005] The first object of the present invention is to provide a hydrogel dressing for promoting wound healing, wherein the hydrogel dressing has excellent mechanical properties and can promote wound healing and inhibit scar formation.

[0006] The second purpose of the present invention is to provide a method for preparing a hydrogel dressing for promoting wound healing, which has simple steps and is easy to put into production.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A hydrogel dressing for promoting wound healing, comprising the following raw materials in parts by weight: 10-18 parts of gel matrix, 1-5 parts of silk fibroin, 0.3-0.8 parts of serpentine, 0.1-0.5 parts of stilbene glycoside, 0.4-0.8 parts of modified soybean lecithin, 0.5-1 parts of trehalose, and 80-100 parts of deionized water;

[0009] The preparation process of the modified soybean lecithin is as follows:

[0010] (1) adding soybean lecithin to a lactic acid solution, adding a hydrogen peroxide solution under heating and stirring conditions, continuing heating and stirring to react, and obtaining a reaction solution after the reaction is completed;

[0011] (2) Adding sodium hydroxide solution to the reaction solution of step (1) until the pH value of the reaction solution is neutral, and vacuum drying the reaction solution to obtain modified soybean lecithin.

[0012] Preferably, the mass fraction of the lactic acid solution is 20-30%, the mass fraction of the hydrogen peroxide solution is 10-20%, and the mass fraction of the sodium hydroxide solution is 1-5%.

[0013] Preferably, the mass ratio of soybean lecithin to lactic acid and hydrogen peroxide in step (1) is 1: (0.01-0.05): (0.1-0.2).

[0014] Preferably, the heating temperature in step (1) is 60-70° C., and the stirring time is 3-5 h.

[0015] Preferably, the gel matrix is ​​composed of chitosan, sodium carboxymethyl cellulose and acetylated distarch phosphate in a mass ratio of 1: (0.5-1): (0.1-0.3).

[0016] The preparation method of the hydrogel dressing for promoting wound healing comprises the following steps:

[0017] The gel matrix, serpentine, diphenyl glycoside, modified soybean lecithin, trehalose and deionized water are uniformly mixed according to the weight ratio, and then silk fibroin is added and irradiated for cross-linking to obtain the product.

[0018] Preferably, the cross-linking time is 20-30 min.

[0019] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0020] The present invention provides a novel hydrogel dressing, which has excellent antibacterial properties and mechanical properties, and can effectively promote wound healing and inhibit scar formation. Ingredients such as serpentine, diphenylethylene glycosides, and modified soybean lecithin are added to the hydrogel dressing formula of the present invention. Among them, the hydroxyl groups in the modified soybean lecithin and the amino groups of components such as chitosan in the gel matrix form hydrogen bonds, thereby improving the mechanical properties of the hydrogel dressing. In addition, the modified soybean lecithin can also increase the water content of the stratum corneum of the skin, which is beneficial to the repair of scars after wound healing. And the results of wound healing experiments show that the combination of serpentine and diphenylethylene glycosides can effectively promote wound healing. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1This is a result analysis diagram of the antibacterial rate of the hydrogel dressings of Examples 1-3 and Comparative Examples 1-7;

[0022] Figure 2 The graphs are the result of the residual areas of the mouse wounds on the 0th day, the 7th day and the 14th day after using the hydrogel dressings of Examples 1-3 and Comparative Examples 1-7;

[0023] Figure 3 This is a result analysis diagram of scar residual width after wound healing using hydrogel dressings of Example 1 and Comparative Examples 1-2. DETAILED DESCRIPTION

[0024] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0025] 1. Preparation example:

[0026] Preparation Example 1:

[0027] Preparation Example 1 provides a modified soybean lecithin, and the detailed preparation process is as follows:

[0028] (1) According to the mass ratio of soybean lecithin, lactic acid and hydrogen peroxide of 1:0.03:0.15, soybean lecithin was added to a 26% lactic acid solution, and then a 14% hydrogen peroxide solution was added under heating and stirring conditions. The mixture was heated and stirred at 65°C for 4 hours. After the reaction was completed, a reaction solution was obtained;

[0029] (2) Adding 2% by mass of sodium hydroxide solution to the reaction solution of step (1) until the pH value of the reaction solution is neutral, and vacuum drying the reaction solution to obtain modified soybean lecithin.

[0030] Preparation Example 2:

[0031] Preparation Example 2 provides a modified soybean lecithin, and the detailed preparation process is as follows:

[0032] (1) According to the mass ratio of soybean lecithin, lactic acid and hydrogen peroxide of 1:0.05:0.2, soybean lecithin was added to a 20% lactic acid solution, and then a 20% hydrogen peroxide solution was added under heating and stirring conditions. The mixture was heated and stirred at 60°C for 5 hours. After the reaction was completed, a reaction solution was obtained;

[0033] (2) Adding a 5% by mass sodium hydroxide solution to the reaction solution of step (1) until the pH value of the reaction solution is neutral, and vacuum drying the reaction solution to obtain modified soybean lecithin.

[0034] Preparation Example 3:

[0035] Preparation Example 3 provides a modified soybean lecithin, and the detailed preparation process is as follows:

[0036] (1) According to the mass ratio of soybean lecithin, lactic acid and hydrogen peroxide of 1:0.01:0.1, soybean lecithin was added to a 30% lactic acid solution, and then a 10% hydrogen peroxide solution was added under heating and stirring conditions. The mixture was heated and stirred at 70°C for 3 hours. After the reaction was completed, a reaction solution was obtained;

[0037] (2) Adding 1% by mass sodium hydroxide solution to the reaction solution of step (1) until the pH value of the reaction solution is neutral, and vacuum drying the reaction solution to obtain modified soybean lecithin.

[0038] 2. Embodiment:

[0039] Embodiment 1:

[0040] Example 1 provides a hydrogel dressing for promoting wound healing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.6 parts of serpentine, 0.4 parts of diphenylethylene glycoside, 0.8 parts of modified soybean lecithin obtained in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water.

[0041] Example 1 also provides a method for preparing the hydrogel dressing for promoting wound healing. The detailed preparation process is as follows:

[0042] According to the above weight ratio, chitosan, sodium carboxymethyl cellulose, acetylated distarch phosphate, serpentine, diphenyl glycoside, modified soybean lecithin, trehalose and deionized water are uniformly mixed, and then silk fibroin is added, and cross-linking is carried out by irradiating with ultraviolet visible light for 30 minutes to obtain the product.

[0043] Embodiment 2:

[0044] Example 2 provides a hydrogel dressing for promoting wound healing, which is composed of the following raw materials in parts by weight: 6.3 parts of chitosan, 3.1 parts of sodium carboxymethyl cellulose, 0.6 parts of acetylated distarch phosphate, 1 part of silk fibroin, 0.3 parts of serpentine, 0.1 parts of diphenylethylene glycoside, 0.7 parts of modified soybean lecithin obtained in Preparation Example 2, 0.5 parts of trehalose, and 80 parts of deionized water.

[0045] Example 2 also provides a method for preparing the hydrogel dressing for promoting wound healing. The detailed preparation process is as follows:

[0046] According to the above weight ratio, chitosan, sodium carboxymethyl cellulose, acetylated distarch phosphate, serpentine, diphenyl glycoside, modified soybean lecithin, trehalose and deionized water are uniformly mixed, and then silk fibroin is added, and irradiated with ultraviolet visible light for 20 minutes for cross-linking to obtain the product.

[0047] Embodiment 3:

[0048] Example 3 provides a hydrogel dressing for promoting wound healing, which is composed of the following raw materials in parts by weight: 7.8 parts of chitosan, 7.8 parts of sodium carboxymethyl cellulose, 2.4 parts of acetylated distarch phosphate, 5 parts of silk fibroin, 0.8 parts of serpentine, 0.5 parts of diphenylethylene glycoside, 0.4 parts of modified soybean lecithin obtained in Preparation Example 3, 1 part of trehalose, and 100 parts of deionized water.

[0049] According to the above weight ratio, chitosan, sodium carboxymethyl cellulose, acetylated distarch phosphate, serpentine, diphenyl glycoside, modified soybean lecithin, trehalose and deionized water are mixed evenly, and then silk fibroin is added, and irradiated with ultraviolet visible light for 25 minutes for cross-linking to obtain the product.

[0050] 3. Comparative example:

[0051] Comparative Example 1:

[0052] Comparative Example 1 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.6 parts of scutellariae, 0.4 parts of diphenylethylene glycosides, 0.8 parts of soybean lecithin, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0053] Comparative Example 2:

[0054] Comparative Example 2 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.6 parts of scutellariae, 0.4 parts of diphenylethylene glycosides, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0055] Comparative Example 3:

[0056] Comparative Example 3 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.6 parts of serpentine, 0.8 parts of modified soybean lecithin prepared in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0057] Comparative Example 4:

[0058] Comparative Example 4 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 1 part of serpentine, 0.8 parts of modified soybean lecithin prepared in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0059] Comparative Example 5:

[0060] Comparative Example 5 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.4 parts of diphenyl glycoside, 0.8 parts of modified soybean lecithin prepared in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0061] Comparative Example 6:

[0062] Comparative Example 6 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 1 part of diphenyl glycoside, 0.8 parts of modified soybean lecithin prepared in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0063] Comparative Example 7:

[0064] Comparative Example 7 provides a hydrogel dressing, which is composed of the following raw materials in parts by weight: 7.9 parts of chitosan, 5.5 parts of sodium carboxymethyl cellulose, 1.6 parts of acetylated distarch phosphate, 3 parts of silk fibroin, 0.8 parts of modified soybean lecithin prepared in Preparation Example 1, 0.8 parts of trehalose, and 92 parts of deionized water. The preparation method of the hydrogel dressing is consistent with that of Example 1.

[0065] The properties of the hydrogel dressings of Examples 1-3 and Comparative Examples 1-7 are described below.

[0066] (1) Mechanical properties of hydrogel dressings:

[0067] The hydrogel dressings obtained in Examples 1-3 and Comparative Examples 1-2 were cut into pieces with a length of 40 mm, a width of 3 mm, and a thickness of 3 mm. The tensile strength and elongation at break of the hydrogel dressings obtained in Examples 1-3 and Comparative Examples 1-2 were tested using a universal mechanical testing machine, and stretched to complete tensile failure at a constant strain rate of 1 mm / min, with each group repeated 5 times. The results were averaged over 5 experiments, and the results are shown in Table 1.

[0068] ;

[0069] It can be seen from Table 1 that, compared with Examples 1-3, Comparative Example 1 uses soybean lecithin instead of modified soybean lecithin, resulting in a significant decrease in the mechanical properties of the hydrogel dressing; Comparative Example 2 does not add modified soybean lecithin, and the mechanical properties of the hydrogel dressing are significantly reduced, and are lower than the mechanical properties of Comparative Example 1. Analysis shows that the hydroxyl groups in the modified soybean lecithin and the amino groups of the components such as chitosan in the gel matrix form hydrogen bonds, thereby improving the mechanical properties of the hydrogel dressing.

[0070] (2) Antibacterial properties of hydrogel dressings:

[0071] The antibacterial properties of the hydrogel dressings of Examples 1-3 and Comparative Examples 1-7 were evaluated by colony forming units (CFU), and the experimental process was as follows:

[0072] The hydrogel dressings obtained in Examples 1-3 and Comparative Examples 1-7 were cut into pieces with a length of 3 cm, a width of 1 cm, and a thickness of 1 mm, and placed in glass test tubes. 10 mL of Escherichia coli and Staphylococcus aureus liquid were added respectively, and the concentration of the bacterial liquid was 1×10 7 CFU / mL, cultured at 35°C, shaker (150rpm) for 3h. After the culture, the culture fluid of each group was taken and the number was counted by plate spreading colony counting method to evaluate the antibacterial effect. In addition, a blank control group was set up. Antibacterial rate = (total number of colonies in the blank control group - total number of colonies in the experimental group) / total number of colonies in the blank control group × 100%, the results are shown in Figure 1 shown.

[0073] Figure 1 The following is an analysis chart of the antibacterial rate of the hydrogel dressings of Examples 1-3 and Comparative Examples 1-7. Figure 1 It can be seen that the hydrogel dressings of Examples 1-3 have excellent antibacterial effects compared to Comparative Examples 1-7. The above results show that changing the ingredients of the hydrogel dressing formula of the present invention will affect the antibacterial effect of the hydrogel dressing.

[0074] (3) Healing properties of hydrogel dressings:

[0075] ①Wound healing properties:

[0076] 100 male mice weighing 18-22 g were randomly divided into 10 groups. A skin defect model was made on the back of the mice using a trephine with a diameter of 10 mm. The wounds were treated with the hydrogels of Examples 1-3 and Comparative Examples 1-7, respectively. The hydrogel dressings were changed once a day, and the residual wound area was observed every day. The results are shown in Table 1. Figure 2 And as shown in Table 2.

[0077] ②Scar residual width:

[0078] After the wound healed, the width of the scar residue of mice in Example 1 and Comparative Example 1-2 groups was calculated. Figure 3 shown.

[0079] ;

[0080] Observe Table 2 and Figure 2 It can be seen that the hydrogel dressings of Examples 1-3 of the present invention can promote wound healing.

[0081] Compared with Examples 1-3, there was no significant difference in the residual area of ​​the animal wounds after treatment with the hydrogel dressings of Comparative Example 1-2.

[0082] Compared with Examples 1-3, the residual area of ​​animal wounds after treatment with Comparative Examples 3-7 hydrogel dressings increased significantly. Among them, the hydrogel dressing formula of Comparative Example 3 did not add diphenylethylene glycosides; the hydrogel dressing formula of Comparative Example 4 did not add diphenylethylene glycosides, but increased the dosage of the scutellaria baicalensis component; the hydrogel dressing formula of Comparative Example 5 did not add scutellaria baicalensis; the hydrogel dressing formula of Comparative Example 6 did not add scutellaria baicalensis, but increased the dosage of diphenylethylene glycosides; the hydrogel dressing formula of Comparative Example 7 did not add scutellaria baicalensis and diphenylethylene glycosides. According to the above results, the combination of scutellaria baicalensis and diphenylethylene glycosides can effectively promote wound healing.

[0083] Figure 3 This is a result analysis chart of scar residual width after wound healing using hydrogel dressings of Example 1 and Comparative Examples 1-2. Figure 3 It can be seen that the residual width of the scar using the hydrogel dressing of Example 1 is significantly reduced compared with Comparative Examples 1-2. However, no modified soybean lecithin is added to the hydrogel dressing formula of Comparative Examples 1-2. According to the above results, it can be seen that the modified soybean lecithin of the present invention can increase the water content of the stratum corneum of the skin, which is beneficial to the repair of scars after wound healing.

[0084] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A hydrogel dressing for promoting wound healing, characterized in that: The invention comprises the following raw materials in parts by weight: 10-18 parts of gel matrix, 1-5 parts of silk fibroin, 0.3-0.8 parts of scutellaria baicalensis, 0.1-0.5 parts of diphenylethylene glycoside, 0.4-0.8 parts of modified soybean lecithin, 0.5-1 parts of trehalose, and 80-100 parts of deionized water; The preparation process of the modified soybean lecithin is as follows: (1) adding soybean lecithin to a lactic acid solution, adding a hydrogen peroxide solution under heating and stirring conditions, continuing heating and stirring to react, and obtaining a reaction solution after the reaction is completed; (2) adding sodium hydroxide solution to the reaction solution of step (1) until the pH value of the reaction solution is neutral, and vacuum drying the reaction solution to obtain modified soybean lecithin; The gel matrix is ​​composed of chitosan, sodium carboxymethyl cellulose and acetylated distarch phosphate in a mass ratio of 1: (0.5-1): (0.1-0.3).

2. The hydrogel dressing for promoting wound healing according to claim 1, characterized in that: The mass fraction of the lactic acid solution is 20-30%, the mass fraction of the hydrogen peroxide solution is 10-20%, and the mass fraction of the sodium hydroxide solution is 1-5%.

3. The hydrogel dressing for promoting wound healing according to claim 1, characterized in that: The mass ratio of soybean lecithin to lactic acid and hydrogen peroxide in step (1) is 1: (0.01-0.05): (0.1-0.2).

4. The hydrogel dressing for promoting wound healing according to claim 1, characterized in that: The heating temperature in step (1) is 60-70° C., and the stirring time is 3-5 h.

5. The method for preparing the hydrogel dressing for promoting wound healing according to any one of claims 1 to 4, characterized in that: The following steps are involved: The gel matrix, serpentine, diphenyl glycoside, modified soybean lecithin, trehalose and deionized water are uniformly mixed according to the weight ratio, and then silk fibroin is added and irradiated for cross-linking to obtain the product.

6. The method for preparing the hydrogel dressing for promoting wound healing according to claim 5, characterized in that: The cross-linking time is 20-30 minutes.

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