A method for extracting active ingredients of Thermus thermophilus in hot spring water and an anti-aging skin composition

Abstract

The present invention provides a method for extracting active ingredients of Thermus thermophilus from hot spring water and an anti-aging skin composition. The extraction method comprises the following steps: (1) inoculating Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus into a fermentation medium at the same time, and culturing the culture at 30-35°C and 120-150r / min to obtain a fermentation culture; (2) separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus from hot spring water. The anti-aging skin composition comprises the following components in mass fractions: 50-55 parts of hot spring water, 20-30 parts of active ingredients of Thermus thermophilus from hot spring water, and 12-22 parts of pentanediol. The anti-aging skin composition of the present invention contains the active ingredients of Thermus thermophilus from hot spring water and has a good anti-aging effect.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and particularly relates to a method for extracting active ingredients of Thermus thermophilus from hot spring water and an anti-aging skin composition. Background Art

[0002] Aging is an inevitable natural law in the process of life. As the first barrier of the human body, the skin is aging all the time. Its elasticity gradually decreases, and it becomes thinner, more fragile and looser, giving people the intuitive impression of wrinkles. Skin aging generally includes natural aging and photoaging. The former mainly refers to the aging caused by irresistible factors such as genetics, gravity, endocrine and immune function in the body with age; the latter is mainly due to the effect of ultraviolet rays, which accelerates the process of natural aging, and a series of environmental exposures such as climate change and environmental pollution also have a direct impact on the photoaging of the skin. The main ways to fight aging include: protecting the skin from external environmental stimuli; removing excess free radicals in cells; repairing and supplementing skin cells. Skin anti-aging is to prevent the gradual functional and organic degenerative changes of the skin over time, which is manifested as preventing wrinkles, dryness, flaking, sagging and spots. Anti-aging cosmetics are cosmetics that achieve anti-aging effects and are one of the important functional cosmetics.

[0003] Skin aging is a natural process in which human functions develop faster or slower due to factors such as the gradual slowing down of cell metabolism, the gradual loss of collagen, environmental pollution, and excessive ultraviolet radiation. The manifestations on the skin mainly include wrinkles and sagging skin. At present, vitamin antioxidants contain antioxidant ingredients that can remove free radicals and promote skin metabolism.

[0004] Therefore, they are added to cosmetics to smooth wrinkles and improve skin texture. However, due to the poor stability of vitamin antioxidants, they are easily oxidized in cosmetics and lose their antioxidant components, so there is a problem that the anti-aging effect of cosmetics is not obvious. Therefore, new breakthroughs are needed in anti-aging products. Summary of the invention

[0005] The purpose of the present invention is to provide a method for extracting active ingredients of Thermus thermophilus from hot spring water and an anti-aging skin composition.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0008] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30-35° C. and 120-150 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 6-8 g of plant extract A, 3-5 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0009] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0010] Furthermore, the preparation method of plant extract A is as follows:

[0011] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-200 mesh sieve to obtain powder A;

[0012] (2) adding 10-13 parts by weight of water and 0.1-0.13 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and performing anaerobic fermentation at a temperature of 32-35° C. for 13-15 hours, followed by sterilization to obtain a first fermentation product A;

[0013] (3) adding 0.15-0.17 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32-35° C. for 7-9 hours, and sterilizing to obtain a second fermentation product A;

[0014] (4) adding 0.18-0.22 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32-35° C. for 10-12 h, and sterilizing to obtain a third fermentation product A;

[0015] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0016] Further, the mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:(3-5):(7-9).

[0017] Furthermore, the preparation method of plant extract B is: mix astragalus, ginkgo leaves and patchouli, crush to 80-100 mesh to obtain powder, mix the powder with deionized water in a volume ratio of 1:20-25, heat and boil at 80-90°C for 2-3h to obtain a medicinal solution, filter, and concentrate to a water content of 4-8wt% to obtain plant extract B.

[0018] Furthermore, the mass ratio of astragalus, ginkgo leaf and patchouli is 1:1.4-1.7:0.3-0.5.

[0019] Furthermore, the inoculation concentration of Thermus thermophilus in the fermentation medium was 10 7 -10 8 CFU / mL.

[0020] Furthermore, the inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 -10 10 CFU / mL.

[0021] Furthermore, the inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 -10 6 CFU / mL.

[0022] An anti-aging skin composition comprises the following components in mass fractions: 50-55 parts of hot spring water, 20-30 parts of active ingredients of Thermus thermophilus in hot spring water, and 12-22 parts of pentylene glycol.

[0023] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. Since plant extract A and plant extract B obtained by extracting multiple plants contain rich active ingredients and other beneficial ingredients, when they are added to the culture medium for fermentation and cultivation of Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus, they can promote the effective extraction and conversion of these ingredients, so that the active ingredients contained in the final fermentation product have better anti-aging effects. These active ingredients can help slow down the skin aging process, improve skin elasticity, reduce the formation of fine lines and wrinkles, and improve the overall health of the skin.

[0026] 2. The simultaneous fermentation of Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus can produce a synergistic effect. During the fermentation process, each microorganism can produce a series of active ingredients that are beneficial to skin health. When they are used for fermentation at the same time, the efficacy of these active ingredients can be further enhanced through synergy, especially for the anti-aging effect of the skin. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1

[0029] This embodiment provides a method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0030] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 6 g of plant extract A, 3 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0031] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0032] The preparation method of plant extract A is as follows:

[0033] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-mesh sieve to obtain powder A;

[0034] (2) adding 10 parts by weight of water and 0.1 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and conducting anaerobic fermentation at a temperature of 32° C. for 13 h, followed by sterilization, to obtain a first fermentation product A;

[0035] (3) adding 0.15 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32° C. for 7 h, and sterilizing to obtain a second fermentation product A;

[0036] (4) adding 0.18 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32° C. for 10 h, and sterilizing to obtain a third fermentation product A;

[0037] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0038] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:3:7.

[0039] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20, heated at 80°C for 2h to obtain a medicinal solution, filtered, and concentrated to a water content of 4wt% to obtain plant extract B.

[0040] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1:1.4:0.3.

[0041] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 CFU / mL.

[0042] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 CFU / mL.

[0043] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 CFU / mL.

[0044] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0045] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0046] Example 2

[0047] This embodiment provides a method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0048] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 35° C. and 150 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 8 g of plant extract A, 5 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0049] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0050] Furthermore, the preparation method of plant extract A is as follows:

[0051] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 200-mesh sieve to obtain powder A;

[0052] (2) adding 13 parts by mass of water and 0.13 parts by mass of Lactobacillus plantarum to 1 part by mass of powder A, and conducting anaerobic fermentation at a temperature of 35° C. for 15 h, followed by sterilization, to obtain a first fermentation product A;

[0053] (3) adding 0.17 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 35° C. for 9 hours, and sterilizing to obtain a second fermentation product A;

[0054] (4) adding 0.22 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 35° C. for 12 h, and sterilizing to obtain a third fermentation product A;

[0055] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0056] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:5:9.

[0057] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 100 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:25, heated at 90°C for 3h to obtain a medicinal solution, filtered, and concentrated to a water content of 8wt% to obtain plant extract B.

[0058] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1: 1.7: 0.5.

[0059] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 8 CFU / mL.

[0060] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 10 CFU / mL.

[0061] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 6 CFU / mL.

[0062] An anti-aging skin composition comprises the following components in mass fractions: 55 parts of hot spring water, 30 parts of active ingredients of Thermus thermophilus in hot spring water, and 22 parts of pentylene glycol.

[0063] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0064] Comparative Example 1

[0065] The difference between this comparative example and Example 1 is that different bacterial species are used.

[0066] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0067] (1) Thermus thermophilus, Lactobacillus brevis and Lactobacillus bulgaricus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 6 g of plant extract A, 3 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0068] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0069] The preparation method of plant extract A is as follows:

[0070] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-mesh sieve to obtain powder A;

[0071] (2) adding 10 parts by weight of water and 0.1 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and conducting anaerobic fermentation at a temperature of 32° C. for 13 h, followed by sterilization, to obtain a first fermentation product A;

[0072] (3) adding 0.15 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32° C. for 7 h, and sterilizing to obtain a second fermentation product A;

[0073] (4) adding 0.18 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32° C. for 10 h, and sterilizing to obtain a third fermentation product A;

[0074] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0075] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:3:7.

[0076] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20, heated at 80°C for 2h to obtain a medicinal solution, filtered, and concentrated to a water content of 4wt% to obtain plant extract B.

[0077] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1:1.4:0.3.

[0078] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 CFU / mL.

[0079] The inoculation concentration of Lactobacillus brevis in the fermentation medium was 10 9 CFU / mL.

[0080] The inoculation concentration of Lactobacillus bulgaricus in the fermentation medium was 10 5 CFU / mL.

[0081] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0082] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0083] Comparative Example 2

[0084] The difference between this comparative example and Example 1 is that the plant extract A is not added.

[0085] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0086] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 9 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0087] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0088] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20, heated at 80°C for 2h to obtain a medicinal solution, filtered, and concentrated to a water content of 4wt% to obtain plant extract B.

[0089] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1:1.4:0.3.

[0090] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 CFU / mL.

[0091] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 CFU / mL.

[0092] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 CFU / mL.

[0093] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0094] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0095] Comparative Example 3

[0096] The difference between this comparative example and Example 1 is that the plant extract B is not added.

[0097] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0098] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 9 g of plant extract A, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0099] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0100] The preparation method of plant extract A is as follows:

[0101] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-mesh sieve to obtain powder A;

[0102] (2) adding 10 parts by weight of water and 0.1 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and conducting anaerobic fermentation at a temperature of 32° C. for 13 h, followed by sterilization, to obtain a first fermentation product A;

[0103] (3) adding 0.15 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32° C. for 7 h, and sterilizing to obtain a second fermentation product A;

[0104] (4) adding 0.18 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32° C. for 10 h, and sterilizing to obtain a third fermentation product A;

[0105] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0106] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:3:7.

[0107] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 CFU / mL.

[0108] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 CFU / mL.

[0109] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 CFU / mL.

[0110] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0111] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0112] Comparative Example 4

[0113] The difference between this comparative example and Example 1 is that the added amounts of plant extract A and plant extract B are different.

[0114] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0115] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 2 g of plant extract A, 7 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0116] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0117] The preparation method of plant extract A is as follows:

[0118] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-mesh sieve to obtain powder A;

[0119] (2) adding 10 parts by weight of water and 0.1 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and conducting anaerobic fermentation at a temperature of 32° C. for 13 h, followed by sterilization, to obtain a first fermentation product A;

[0120] (3) adding 0.15 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32° C. for 7 h, and sterilizing to obtain a second fermentation product A;

[0121] (4) adding 0.18 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32° C. for 10 h, and sterilizing to obtain a third fermentation product A;

[0122] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0123] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:3:7.

[0124] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20, heated at 80°C for 2h to obtain a medicinal solution, filtered, and concentrated to a water content of 4wt% to obtain plant extract B.

[0125] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1:1.4:0.3.

[0126] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 CFU / mL.

[0127] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 CFU / mL.

[0128] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 CFU / mL.

[0129] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0130] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0131] Comparative Example 5

[0132] The difference between this comparative example and Example 1 is that the added concentration of the bacteria is different.

[0133] A method for extracting active ingredients of Thermus thermophilus from hot spring water, the extraction method comprising the following steps:

[0134] (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30° C. and 120 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 6 g of plant extract A, 3 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium;

[0135] (2) Separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water.

[0136] The preparation method of plant extract A is as follows:

[0137] (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-mesh sieve to obtain powder A;

[0138] (2) adding 10 parts by weight of water and 0.1 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and conducting anaerobic fermentation at a temperature of 32° C. for 13 h, followed by sterilization, to obtain a first fermentation product A;

[0139] (3) adding 0.15 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32° C. for 7 h, and sterilizing to obtain a second fermentation product A;

[0140] (4) adding 0.18 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32° C. for 10 h, and sterilizing to obtain a third fermentation product A;

[0141] (5) The third fermentation product A is filtered through a 0.22 μm filter membrane to obtain a filtrate, and the filtrate is concentrated and dried to obtain a plant extract A.

[0142] The mass ratio of red raspberry leaves, pomegranate peel and gallnut is 10:3:7.

[0143] The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80 mesh to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20, heated at 80°C for 2h to obtain a medicinal solution, filtered, and concentrated to a water content of 4wt% to obtain plant extract B.

[0144] The mass ratio of Astragalus, Ginkgo Biloba and Patchouli is 1:1.4:0.3.

[0145] The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 5 CFU / mL.

[0146] The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 11 CFU / mL.

[0147] The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 9 CFU / mL.

[0148] An anti-aging skin composition comprises the following components in mass fractions: 50 parts of hot spring water, 20 parts of active ingredients of Thermus thermophilus in hot spring water, and 12 parts of pentylene glycol.

[0149] The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

[0150] Performance Testing

[0151] Anti-aging test: Take 5 mg of DPPH (1,1-diphenyl-2-picrylhydrazyl (free radical)) and dissolve it in 100 ml of solvent (anhydrous ethanol), dissolve it by ultrasonic for 5 minutes, and mix it thoroughly. Take 5 ml of the DPPH solution and check the absorbance A value at 519 nm. The A value is best between 1.2-1.3, which means that the DPPH standard solution is prepared.

[0152] The anti-aging skin composition of the embodiment and the comparative example was used as the sample solution to be tested, 5 ml of the sample solution was added into 5 ml of the DPPH standard solution and mixed evenly, and placed in a dark room for reaction at room temperature for 0.5 h, 7.5 ml of the sample solution was added into 10 ml of anhydrous ethanol solution and mixed evenly, and the absorbance value was tested at 519 nm.

[0153] DPPH free radical scavenging rate (%) = 1- (A2 / A1) × 100%; A1: absorbance value of a mixed solution consisting of a DPPH solution and anhydrous ethanol; A2: absorbance value of a mixed solution consisting of a DPPH solution and an ethanol solution, and the sample solutions of the embodiments and comparative examples.

[0154] Table 1 Performance test results

[0155]

[0156] It can be seen from the above performance test results that Examples 1-2 have good anti-aging effects, while the comparative examples do not adopt the necessary technical solutions, resulting in their corresponding performance tests being significantly worse than those of the embodiments. The above experimental results further demonstrate the importance of the technical solutions defined in the present invention to its technical effects.

[0157] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for extracting active ingredients from Thermus thermophilus in hot spring water, characterized in that: The extraction method comprises the following steps: (1) Thermus thermophilus, Bifidobacterium longum and Lactobacillus helveticus are inoculated into a fermentation medium at the same time, and the fermentation culture is carried out at 30-35° C. and 120-150 r / min to obtain a fermentation culture, wherein the fermentation medium is prepared by the following method per liter: 300 g of potato is boiled in 500 mL of hot spring water for 20 min, the juice is filtered, 20 g of glucose, 6-8 g of plant extract A, 3-5 g of plant extract B, 3 g of KH2PO4, 0.75 g of MgSO4 and 10 mg of vitamin B1 are added, and the volume is supplemented to 1 L with water, and the pH is natural to obtain a fermentation medium; The inoculum concentration of Thermus thermophilus in the fermentation medium was 10 7 -10 8 CFU / mL; The inoculation concentration of Bifidobacterium longum in the fermentation medium was 10 9 -10 10 CFU / mL; The inoculation concentration of Lactobacillus helveticus in the fermentation medium was 10 5 -10 6 CFU / mL; (2) separating mycelium and fermentation liquid from the fermentation culture, extracting the fermentation liquid with ethyl acetate, and concentrating the extract to obtain the active ingredients of Thermus thermophilus in hot spring water; The preparation method of plant extract A is as follows: (1) Mix dried red raspberry leaves, pomegranate peels and Chinese gallnut, and pass through a 100-200 mesh sieve to obtain powder A; wherein the mass ratio of red raspberry leaves, pomegranate peels and Chinese gallnut is 10:(3-5):(7-9); (2) adding 10-13 parts by weight of water and 0.1-0.13 parts by weight of Lactobacillus plantarum to 1 part by weight of powder A, and performing anaerobic fermentation at a temperature of 32-35° C. for 13-15 hours, followed by sterilization to obtain a first fermentation product A; (3) adding 0.15-0.17 parts by weight of Lactobacillus brevis to the first fermentation product A for anaerobic fermentation at a temperature of 32-35° C. for 7-9 hours, and sterilizing to obtain a second fermentation product A; (4) adding 0.18-0.22 parts by weight of Lactobacillus helveticus to the second fermentation product A for anaerobic fermentation at a temperature of 32-35° C. for 10-12 h, and sterilizing to obtain a third fermentation product A; (5) filtering the third fermentation product A through a 0.22 μm filter membrane to obtain a filtrate, and concentrating and drying the filtrate to obtain a plant extract A; The preparation method of plant extract B is as follows: astragalus, ginkgo leaves and patchouli are mixed, crushed to 80-100 meshes to obtain powder, the powder is mixed with deionized water in a volume ratio of 1:20-25, heated at 80-90°C for 2-3h to obtain a medicinal solution, filtered, and concentrated to a water content of 4-8wt% to obtain plant extract B; wherein the mass ratio of astragalus, ginkgo leaves and patchouli is 1:1.4-1.7:0.3-0.

5.

2. An anti-aging skin composition, characterized in that The invention comprises the following components in mass fractions: 50-55 parts of hot spring water, 20-30 parts of active ingredients of Thermus thermophilus in hot spring water obtained by the method for extracting active ingredients of Thermus thermophilus in hot spring water as claimed in claim 1, and 12-22 parts of pentanediol.

3. The anti-aging skin composition according to claim 2, characterized in that The preparation method of the anti-aging skin composition comprises the following steps: mixing hot spring water, active ingredients of Thermus thermophilus in hot spring water, and pentylene glycol, and stirring the mixture evenly.

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