A water quality regulator for aquaculture and its preparation method

Abstract

The present invention discloses a special water quality regulator for aquaculture and its preparation method, which relates to the technical field of aquaculture. The special water quality regulator for aquaculture of the present invention comprises the following raw material components in parts by mass: 8-15 parts of compound microorganisms, 35-80 parts of clay ore, 15-20 parts of water extract of sunflower leaves, 8-12 parts of water buttercup extract, 2-3 parts of picroside II, 5-6 parts of glucose, and 0.02-0.04 parts of ferrous chloride; the compound microorganisms are obtained by compounding bacillus cereus and acinetobacter johnsonii in a mass ratio of 1-2:1. The preparation method of the special water quality regulator for aquaculture of the present invention is simple, the raw materials are easy to obtain, and the purification effect of the aquaculture water body is excellent, having an important application prospect in the purification of aquaculture water bodies.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and particularly relates to a special water quality regulator for aquaculture and a preparation method thereof. Background Art

[0002] Phenomena such as water pollution and the breeding of pests and diseases seriously restrict the healthy and stable development of the aquaculture industry. At the same time, the water pollution problem caused by aquaculture will further have an adverse impact on the ecological environment, and thus cause varying degrees of damage to the environmental waters.

[0003] Generally, physical, chemical or biological methods are used to improve the internal environment of the aquaculture water body, achieve the purification effect of aquaculture, and improve the aquaculture capacity. Among them, the purification effect of the physical purification method on the aquaculture water body is limited and the purification effect is not ideal; although the chemical purification method has a quick effect, it is easy to cause new water body chemical pollution problems; the biological purification method has important applications in the water quality purification of aquaculture based on its green and effective characteristics.

[0004] There are a large number of microorganisms in the aquaculture water body. These microorganisms are diverse and constitute a complex microecosystem. However, under natural conditions, these microorganisms are difficult to meet the water body bioremediation effect, and adding specific types of exogenous microorganisms to the aquaculture water body is an important technical means.

[0005] However, although adding exogenous microorganisms to the aquaculture water body is an effective means, how to ensure the normal metabolic activities of exogenous microorganisms in the water body and at the same time ensure their interaction with the original microorganisms in the water body is the technical key to determining the bioremediation effect of the aquaculture water body. Summary of the Invention

[0006] The purpose of the present invention is to provide a special water quality regulator for aquaculture and a preparation method thereof to solve the problems existing in the above-mentioned prior art, realize the water quality regulation of the aquaculture water body, and promote the efficient and stable development of the aquaculture industry.

[0007] To achieve the above purpose, the present invention provides the following scheme:

[0008] The present invention provides a special water quality regulator for aquaculture, which comprises the following raw material components in parts by mass:

[0009] 8-15 parts of complex microorganisms, 35-80 parts of clay minerals, 15-20 parts of water extract of sunflower leaves, 8-12 parts of extract of Batrachium bungei, 2-3 parts of picroside II, 5-6 parts of glucose, and 0.02-0.04 parts of ferrous chloride;

[0010] The complex microorganisms are obtained by compounding Bacillus cereus and Acinetobacter johnsonii in a mass ratio of 1-2:1.

[0011] Further, the clay mineral is one or more of montmorillonite, vermiculite or sepiolite.

[0012] Further, the preparation process of the sunflower leaf aqueous extract includes the following steps:

[0013] a. Heating and refluxing the sunflower leaves with ethanol, concentrating the extract, and drying to obtain the crude sunflower leaf extract;

[0014] b. Soaking the crude sunflower leaf extract in an aqueous glycerol solution, centrifuging and filtering after soaking, and concentrating to obtain a concentrated solution;

[0015] c. Extracting the concentrated solution with petroleum ether, collecting the extracted aqueous phase, and drying to obtain the sunflower leaf aqueous extract.

[0016] Further, the concentration of the aqueous glycerol solution is 10 - 15 wt%.

[0017] Further, the soaking time is 2 - 3 h.

[0018] Further, the preparation process of the buttercup extract includes the following steps:

[0019] Performing ethanol ultrasonic extraction on the buttercup, concentrating the extract, and drying to obtain the buttercup extract.

[0020] Further, the concentration temperature is 30 - 40 °C; the drying temperature is 30 - 40 °C.

[0021] The present invention also provides a preparation method of the above - mentioned special water quality regulator for aquaculture, including the following steps:

[0022] Mixing the clay, sunflower leaf aqueous extract, buttercup extract, pectolinarigenin, glucose and ferrous chloride according to the mass - part ratio, and adding the composite microorganism to obtain the special water quality regulator for aquaculture.

[0023] The present invention further provides an application of the above - mentioned special water quality regulator for aquaculture in purifying aquaculture water bodies.

[0024] Further, when using the special water quality regulator for aquaculture of the present invention to purify aquaculture water bodies, the special water quality regulator for aquaculture is added according to an addition amount of 0.02 - 0.1‰ of the weight of the aquaculture water body.

[0025] The present invention discloses the following technical effects:

[0026] In view of the purification problem of aquaculture water bodies, the present invention provides a special water quality regulator for aquaculture containing a compound strain of Bacillus cereus and Acinetobacter johnsonii. The strains used in this water quality regulator are reasonably compounded, avoiding the adverse effects of exogenous microorganisms and indigenous microorganisms in aquaculture water bodies, and can ensure excellent aquaculture water quality purification effects.

[0027] On the one hand, the clay mineral in the components of the present invention can play an adsorption and purification effect, and on the other hand, it can also provide an attachment site for microorganisms; the water extract of sunflower leaves, the extract of Batrachium bungei, and aucubin can significantly promote the metabolic activity of the composite bacteria, which is the key to ensuring the purification of aquaculture water quality. At the same time, glucose and ferrous chloride can further ensure the nutrients required for the growth and metabolism of microorganisms during the storage of the water quality regulator.

[0028] The preparation method of the special water quality regulator for aquaculture of the present invention is simple, the raw materials are easy to obtain, and the purification effect of aquaculture water bodies is excellent, having an important application prospect in the purification of aquaculture water bodies. Detailed Embodiments

[0029] The various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation embodiments of the present invention.

[0030] It should be understood that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0031] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0032] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the present invention specification, which are obvious to those skilled in the art. Other embodiments obtained from the specification of the present invention are obvious to those skilled in the art. The specification and embodiments of the present invention are merely exemplary.

[0033] As used herein, terms such as "comprising", "including", "having", "containing", etc. are all open-ended terms, meaning including but not limited to.

[0034] In the following examples of the present invention, Bacillus cereus used was purchased from the China Center for Type Culture Collection, with the preservation number: CCTCC NO: M2023699; Acinetobacter johnsonii was purchased from the China Center for Type Culture Collection, with the preservation number: CCTCC NO: M 20221809.

[0035] Example 1

[0036] A special water quality regulator for aquaculture has the following raw material components:

[0037] 12 parts of compound microorganisms, 55 parts of sepiolite, 18 parts of water extract of sunflower leaves, 10 parts of extract of Batrachium bungei, 3 parts of pectolinarigenin, 6 parts of glucose, and 0.03 parts of ferrous chloride.

[0038] Among them, the compound microorganisms are obtained by compounding Bacillus cereus and Acinetobacter johnsonii in a mass ratio of 2:1.

[0039] (1) The extraction steps of the water extract of sunflower leaves are as follows:

[0040] a. Heat the washed sunflower leaves under reflux with ethanol for 2 h, concentrate the extract, and dry to obtain the crude extract of sunflower leaves;

[0041] b. Soak the crude extract of sunflower leaves in a glycerol aqueous solution (15 wt%) for 2 h. After soaking, centrifuge and filter, and concentrate to obtain a concentrated solution;

[0042] c. Extract the concentrated solution with petroleum ether, collect the extraction aqueous phase, and dry to obtain the water extract of sunflower leaves.

[0043] (2) The extraction steps of the extract of Batrachium bungei are as follows:

[0044] Wash the Batrachium bungei, dry and crush it, add anhydrous ethanol, extract by ultrasonic for 60 min, and then concentrate and dry under reduced pressure at 40 °C to obtain the extract of Batrachium bungei.

[0045] (3) Preparation of the special water quality regulator for aquaculture:

[0046] First, mix sepiolite, the water extract of sunflower leaves, the extract of Batrachium bungei, pectolinarigenin, glucose, and ferrous chloride according to the mass ratio, and then add the compound microorganisms to the obtained mixture and stir evenly again to obtain the special water quality regulator for aquaculture.

[0047] Example 2

[0048] A water quality regulator for aquaculture, the raw material components are as follows:

[0049] 13 parts of complex microorganisms, 65 parts of montmorillonite, 18 parts of water extract of sunflower leaves, 10 parts of water extract of buttercup, 2 parts of pectolinarigenin, 5 parts of glucose, and 0.04 parts of ferrous chloride.

[0050] Among them, the complex microorganisms are obtained by compounding Bacillus cereus and Acinetobacter johnsonii in a mass ratio of 1:1.

[0051] (1) The extraction steps of the water extract of sunflower leaves are as follows:

[0052] a. Heat the washed sunflower leaves under reflux with ethanol for 2 h, concentrate the extract, and dry to obtain the crude extract of sunflower leaves;

[0053] b. Soak the crude extract of sunflower leaves in a glycerol aqueous solution (15 wt%) for 3 h. After soaking, centrifuge and filter, and concentrate to obtain a concentrated solution;

[0054] c. Extract the concentrated solution with petroleum ether, collect the extracted aqueous phase, and dry to obtain the water extract of sunflower leaves.

[0055] (2) The extraction steps of the water extract of buttercup are as follows:

[0056] Wash the buttercup, dry and crush it, add anhydrous ethanol, extract by ultrasonic for 40 min, and then concentrate and dry under reduced pressure at 35 °C to obtain the water extract of buttercup.

[0057] (3) Preparation of the water quality regulator for aquaculture:

[0058] First, mix montmorillonite, the water extract of sunflower leaves, the water extract of buttercup, pectolinarigenin, glucose, and ferrous chloride according to the mass ratio, and then add the complex microorganisms to the obtained mixture and stir evenly again to obtain the water quality regulator for aquaculture.

[0059] Example 3

[0060] A water quality regulator for aquaculture, the raw material components are as follows:

[0061] 8 parts of complex microorganisms, 35 parts of vermiculite, 15 parts of water extract of sunflower leaves, 12 parts of water extract of buttercup, 2 parts of pectolinarigenin, 5 parts of glucose, and 0.02 parts of ferrous chloride.

[0062] Among them, the complex microorganisms are obtained by compounding Bacillus cereus and Acinetobacter johnsonii in a mass ratio of 2:1.

[0063] (1) The extraction steps of the water extract of sunflower leaves are as follows:

[0064] a. Heat the washed sunflower leaves under reflux with ethanol for 1.5 h, concentrate the extract, and dry it to obtain the crude sunflower leaf extract;

[0065] b. Soak the crude sunflower leaf extract in an aqueous glycerol solution (15 wt%) for 2 h. After soaking, centrifuge and filter, and concentrate to obtain a concentrated solution;

[0066] c. Extract the concentrated solution with petroleum ether, collect the extracted aqueous phase, and dry it to obtain the water extract of sunflower leaves.

[0067] (2) The extraction steps of the buttercup extract are as follows:

[0068] Wash the buttercup, dry and crush it, add absolute ethanol, ultrasonically extract for 55 min, then concentrate and dry under reduced pressure at 40 °C to obtain the buttercup extract.

[0069] (3) Preparation of a special water quality regulator for aquaculture:

[0070] First, mix vermiculite, the water extract of sunflower leaves, the buttercup extract, pectolinarigenin, glucose, and ferrous chloride according to the mass ratio, and then add a compound microorganism to the obtained mixture and stir evenly again to obtain a special water quality regulator for aquaculture.

[0071] Comparative Example 1

[0072] The difference from Example 1 is only that the sunflower leaves in step a are replaced by sunflower flower heads.

[0073] Use the obtained water extract of sunflower flower heads to replace the water extract of sunflower leaves in Example 1 in equal amount.

[0074] Comparative Example 2

[0075] The difference from Example 1 is only that the sunflower leaves are extracted with absolute ethanol:

[0076] Wash the sunflower leaves, dry and crush them, add absolute ethanol, ultrasonically extract for 60 min, then concentrate and dry under reduced pressure at 40 °C to obtain the sunflower leaf extract.

[0077] Use the obtained sunflower leaf extract to replace the water extract of sunflower leaves in Example 1 in equal amount.

[0078] Comparative Example 3

[0079] The difference from Example 1 is only that Acinetobacter johnsonii is replaced by an equal mass of Bacillus cereus.

[0080] Comparative Example 4

[0081] The difference from Example 1 is only that pectolinarigenin is replaced by an equal mass of glucose.

[0082] Effect verification example 1:

[0083] According to the addition amount of 0.05‰ of the weight of the aquaculture water body, the water quality regulators prepared in Examples 1-3 and Comparative Examples 1-4 were evenly sprinkled into the tilapia aquaculture ponds, and were used as Experimental Groups 1-3 and Control Experimental Groups 1-4 respectively.

[0084] The tilapia in each group was in good health, with a weight of 400-450 g per fish, and the aquaculture density was 25 kg / m 3 , and the water temperature in the aquaculture pond was 24-25 °C. After 7 days of sprinkling the water quality regulator, the treatment effects of the aquaculture water bodies in each group were measured, and the results are shown in Table 1.

[0085] Table 1

[0086]

[0087] Effect verification example 2

[0088] According to the addition amount of 0.05‰ of the weight of the aquaculture water body, the water quality regulators prepared in Examples 1-3 and Comparative Examples 1-4 were evenly sprinkled into the South American white shrimp aquaculture ponds, and were used as Experimental Groups 1-3 and Control Experimental Groups 1-4 respectively.

[0089] The South American white shrimp in each group was in good health, with an average body length of 300 mm, and the aquaculture density was 200 shrimps / m 3 , and the water temperature in the aquaculture pond was 29-30 °C. After 7 days of sprinkling the water quality regulator, the treatment effects of the aquaculture water bodies in each group were measured, and the results are shown in Table 2.

[0090] Table 2

[0091]

[0092] The above-described embodiments are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A water quality regulator special for aquaculture, characterized in that, Comprising the following raw material components in parts by mass: 8 - 15 parts of compound microorganisms, 35 - 80 parts of clay mineral, 15 - 20 parts of water extract of sunflower leaves, 8 - 12 parts of water buttercup extract, 2 - 3 parts of pectolinarigenin, 5 - 6 parts of glucose, and 0.02 - 0.04 parts of ferrous chloride; The compound microorganisms are obtained by compounding Bacillus cereus and Acinetobacter johnsonii in a mass ratio of 1 - 2:1; The preparation process of the water extract of sunflower leaves includes the following steps: a. Heating and refluxing the sunflower leaves with ethanol, concentrating the extract, and drying to obtain the crude extract of sunflower leaves; b. Soaking the crude extract of sunflower leaves in an aqueous glycerol solution, after the soaking ends, centrifuging and filtering, and concentrating to obtain a concentrated solution; c. Extracting the concentrated solution with petroleum ether, collecting the extraction aqueous phase, and drying to obtain the water extract of sunflower leaves.

2. The water quality regulator for aquaculture according to claim 1, characterized in that The clay mineral is one or more of montmorillonite, vermiculite, or sepiolite.

3. The water quality regulator for aquaculture according to claim 1, characterized in that, The concentration of the aqueous glycerol solution is 10 - 15 wt%.

4. The water quality regulator for aquaculture according to claim 3, characterized in that, The soaking time is 2 - 3 h.

5. The water quality regulator for aquaculture according to claim 1, characterized in that, The preparation process of the water buttercup extract includes the following steps: Performing ethanol ultrasonic extraction on water buttercup, concentrating the extract, and drying to obtain the water buttercup extract.

6. The water quality regulator for aquaculture according to claim 1, wherein The concentration temperature is 30 - 40 °C; the drying temperature is 30 - 40 °C.

7. The preparation method of the special water quality regulator for aquaculture according to any one of claims 1-6, characterized in that, Including the following steps: Mixing the clay mineral, water extract of sunflower leaves, water buttercup extract, pectolinarigenin, glucose, and ferrous chloride according to the parts by mass ratio, and adding the compound microorganisms to obtain the special water quality regulator for aquaculture.

8. Use of the special water quality regulator for aquaculture according to any one of claims 1 - 6 in purifying aquaculture water bodies.

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