A method for producing biological organic fertilizer under forest

Abstract

The present disclosure provides a method for producing biological organic fertilizer under forests. The method includes: culturing the strain of white rot fungus WH‑B‑X3 in the first straw to obtain a first-level solid strain; inoculating the first-level solid strain on the second straw to obtain a second-level solid strain; adding the second-level solid strain to the second liquid strain culture medium in a large fermentation tank to obtain a third-level liquid strain; inoculating the third-level liquid strain on the third straw, and laying the inoculated third straw on the soil under the forest for cultivation to obtain biological organic fertilizer. When the white rot fungus WH‑B‑X3 is used to produce biological organic fertilizer, the straw can be locally sourced, the straw is directly crushed and sprayed in the field, the third straw is obtained, and the inoculation is placed under the forest for aerobic fermentation, and the preparation of biological organic fertilizer can be realized, and the production process is simple, the cost is low, and it is conducive to wide promotion and application.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a method for producing bio-organic fertilizer under forests. Background Art

[0002] Bio-organic fertilizer refers to a type of fertilizer that combines the effects of microorganisms and organic fertilizers, which are made from specific functional microorganisms and organic materials that are mainly derived from animal and plant residues (such as livestock and poultry manure, crop straw, etc.) and have been harmlessly treated and decomposed. Compared with chemical fertilizers, bio-organic fertilizers are widely used because they have a complete range of nutrients, can improve soil, and significantly improve product quality.

[0003] However, the traditional organic fertilizer production is to mix straw and animal feces, and then use high temperature anaerobic composting fermentation technology. During the high temperature anaerobic fermentation process, a large amount of harmful gases and sewage are discharged. When using normal temperature aerobic fermentation for factory production of biological organic fertilizer, sewage and harmful gases will not be produced. However, when using crop straw as raw material, the straw needs to be sterilized to ensure the inoculation effect; at the same time, in order to avoid repeated turning of the pile for heat dissipation and oxygen supply, resulting in infection and interruption of the fermentation process, it is necessary to ferment in a specific patented fermentation box, which makes the process of producing biological organic fertilizer complicated and costly, and at the same time, limits the development of using crop straw as raw material to produce biological organic fertilizer. Therefore, there is an urgent need for a method for producing biological organic fertilizer using crop straw as raw material without pollution, simple process, low cost and high efficiency.

[0004] Public Content

[0005] In order to solve the problems of the prior art, the present disclosure provides a method for producing bio-organic fertilizer under forests. The technical solution is as follows:

[0006] In one aspect, the present disclosure provides a method for producing bio-organic fertilizer under forests, the method comprising:

[0007] Cultivating a strain of white rot fungus WH-B-X3 in the first straw, wherein the white rot fungus WH-B-X3 was deposited in China Center for Type Culture Collection on May 8, 2024, with a deposit address of Wuhan University, Wuhan, China, with a deposit number of CCTCC NO: M 2024884, and is classified and named as white rot fungus WH-B-X3 Pleurotus ostreatus WH-B-X3, to obtain a primary solid strain;

[0008] or inoculating the white rot fungus WH-B-X3 into the first liquid culture medium to obtain a primary liquid culture medium;

[0009] Inoculating the first-level solid bacterial strain or the first-level liquid bacterial strain on the second straw for cultivation to obtain a second-level solid bacterial strain;

[0010] Adding the secondary solid bacterial strain into the second liquid bacterial strain culture medium in the large fermentation tank for cultivation to obtain a tertiary liquid bacterial strain;

[0011] The third-level liquid bacteria are inoculated on the third straw, and the inoculated third straw is laid on the soil under the forest for fermentation to obtain the biological organic fertilizer.

[0012] Specifically, a sunshade layer is covered on the surface of the third straw after inoculation in the light exposure.

[0013] Furthermore, the surface of the third straw after inoculation in the light is covered with at least one of a sunshade net, soil, branches and grass.

[0014] Specifically, the method also includes pre-treatment of the first straw, the second straw and the third straw, crushing the first straw and the second straw to 40-60 mesh, crushing the third straw to 2-10 cm, and then spraying and infiltrating to make the moisture content of the first straw, the second straw and the third straw 55-67%.

[0015] Specifically, the method also includes using a ventilation device to cool down and supply oxygen to the fermenting third straw, the ventilation device includes: a first ventilation pipe and a plurality of second ventilation pipes, the plurality of second ventilation pipes are arranged at intervals, and the plurality of second ventilation pipes are vertically installed on the first ventilation pipe, the first ventilation pipe and the second ventilation pipe are both provided with a plurality of ventilation holes, the first ventilation pipe is arranged between the inoculated third straw and the soil under the forest, and the third straw is extended from both ends of the first ventilation pipe and the top of the second ventilation pipe.

[0016] Specifically, the first straw, the second straw and the third straw are respectively at least one of corn straw, wheat straw, rape straw and peanut straw.

[0017] Specifically, the temperature under the forest is 12-35°C.

[0018] Specifically, the humidity under the forest is 60-90%.

[0019] Furthermore, when the humidity under the forest is lower than 65%, the humidity is increased by watering or spraying the third straws.

[0020] Specifically, the third straws are laid on the soil under the forest to form ridges, and the ridge height is 20 to 40 cm.

[0021] Specifically, the liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of the soybean meal powder, the molasses, the potassium dihydrogen phosphate, the magnesium sulfate and the water are: 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts respectively.

[0022] Specifically, the white rot fungus WH-B-X3 and the Bacillus associated with the white rot fungus WH-B-X3 are cultured together in the first straw to obtain a primary solid fungus;

[0023] Or the strain of the white rot fungus WH-B-X3 and the Bacillus associated with the white rot fungus WH-B-X3 are inoculated into a liquid strain culture medium to obtain a primary liquid strain.

[0024] The beneficial effects brought about by the technical solution provided by the embodiment of the present disclosure are as follows: the white rot fungus WH-B-X3 provided in the embodiment of the present disclosure belongs to the genus of wood-rotting fungi, which is collected from rotten wood in the Shennongjia primeval forest, and is purified and optimized. It is repeatedly cultivated and purified against infection by miscellaneous bacteria on both non-moldy and moldy straws to obtain the white rot fungus WH-B-X3 provided by the embodiment of the present invention. The white rot fungus WH-B-X3 can be directly inoculated on the third straw that has not been sterilized for cultivation. The white rot fungus WH-B-X3 attaches to the third straw and grows well. It can be seen that the white rot fungus WH-B-X3 provided by the embodiment of the present invention has excellent anti-bacterial ability, and the white rot fungus WH-B-X3 is non-toxic and edible, has a fast germination speed, and has a strong lignin decomposition ability; when the white rot fungus WH-B-X3 is used to produce biological organic fertilizer, the straw can be locally sourced, the straw is directly crushed and sprayed in the field to obtain the third straw, and then the third-level liquid strain is inoculated on the unsterilized third straw and placed under the forest for aerobic fermentation, so as to realize the preparation of biological organic fertilizer Its production process has no sewage and harmful gas emissions, is simple to operate, and has low cost. It can reduce the fertilizer input of fruit farmers and has universal applicability. In the process of fermentation of the three-level liquid bacteria, the lignin of the third straw is continuously decomposed and can be taken as needed. Its production cycle is short and the effect is quick. At the same time, the oyster mushroom can be produced after 25 days. After the white rot fungus WH-B-X3 is produced and mature, it can be harvested. The yield of the oyster mushroom per mu can reach 4,000 catties. The produced oyster mushrooms are non-toxic and edible, and can be sold after harvesting, which can increase farmers' income. After the mushrooms are grown, the lignin and cellulose in the third straw are fully decomposed, making the biological organic fertilizer rich in organic matter, nitrogen, phosphorus, potassium, active bacteria and a large amount of humic acid, providing high-quality multifunctional biological organic fertilizer for fruit trees, which can improve fruit yield and quality; the third straw after the mushrooms are grown is plowed into the ground. Under the action of active bacteria and humic acid, it can adsorb, separate and passivate heavy metals in the soil, and can also adsorb and decompose plastic particles in the soil to improve soil properties. At the same time, the hyphae of the white rot fungus WH-B-X3 will release organic chemicals such as 3-octanone, which has a good killing effect on pests such as nematodes, and can improve fruit yield and quality. At the same time, the third straw is laid in the soil under the forest, which can effectively inhibit the growth of weeds in the soil under the forest, and can also achieve the effect of moisture conservation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1is a schematic diagram of the use structure of the ventilation device provided in the first embodiment of the present disclosure;

[0027] Figure 2 is a schematic diagram of the structure of the ventilation device provided in the first embodiment of the present disclosure;

[0028] Figure 3 This is a photo of the white rot fungus WH-B-X3 produced under the forest provided in Example 1 of the present disclosure;

[0029] Figure 4 This is a photograph of the biological organic fertilizer after the harvest of Pleurotus ostreatus provided in Example 1 of the present disclosure. DETAILED DESCRIPTION

[0030] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the embodiments of the present disclosure will be further described in detail below with reference to the accompanying drawings.

[0031] Embodiment 1

[0032] The present invention provides a method for producing bio-organic fertilizer under forests, the method comprising:

[0033] The white rot fungus WH-B-X3 was cultured in the first straw, and the white rot fungus WH-B-X3 was deposited in the China Center for Type Culture Collection on May 8, 2024, with the deposit address being Wuhan University, Wuhan, China, and the deposit number being CCTCC NO: M2024884, to obtain a primary solid strain;

[0034] Inoculating the first-level solid bacterial strain on the second straw for cultivation to obtain the second-level solid bacterial strain;

[0035] The second-level solid bacterial strain is added to the second liquid bacterial strain culture medium in the large fermentation tank for cultivation to obtain the third-level liquid bacterial strain;

[0036] The third-level liquid bacteria are inoculated on the third straw, and the inoculated third straw is spread on the soil under the forest for fermentation to obtain biological organic fertilizer.

[0037] Specifically, the straw is crushed, dusted and infiltrated to obtain the first straw, and the straw is crushed to 40-60 meshes. In this embodiment, the moisture content of the first straw after infiltration is 62-67%. The first straw is put into a triangular bottle with a magnetic rotor, and the triangular bottle is sterilized in a steam sterilization furnace to obtain a first-level straw solid bacterial culture medium; the sterilization temperature is 121-123° C., the sterilization pressure is 0.1 Mpa, and the sterilization time is 30-50 min. After sterilization, the temperature is reduced to 25-28° C. and can be used for subsequent operations;

[0038] The strain of white rot fungus WH-B-X3 cultured on the plate is inoculated into a triangular flask containing a first-level straw solid strain culture medium, with an inoculation ratio of 2-10%, and cultured in an incubator at a temperature of 25-30°C for 5-9 days to obtain a first-level solid strain;

[0039] In the clean bench, add appropriate amount of sterilized water to the triangular flask containing the magnetic rotor and the primary straw solid culture medium for dilution, stir on a magnetic stirrer for 20 to 40 seconds, and set aside.

[0040] The straw is crushed, dusted and soaked to obtain a second straw. In this embodiment, the moisture content of the soaked second straw is 62-67%. The second straw is sterilized at a temperature of 121-123° C., a pressure of 0.1 MPa and a time of 30-50 min. After sterilization, the temperature is reduced to 25-28° C. and can be used for subsequent operations.

[0041] The sterilized second straw is loaded into a solid bacterial culture fermentation vessel with application number CN202220918864.1, water is added and stirred evenly to make the water content 62-67%, and steam high-temperature sterilization is performed, the temperature is set at 121-123°C, the time is set at 30-50 minutes, and after the sterilization is completed, the temperature is lowered to 25-28°C, and it is used as a solid bacterial culture medium for standby use;

[0042] The first-level solid bacteria are inoculated into a solid bacteria fermentation vessel containing a solid bacteria culture medium at an inoculation rate of 2% to 10%, the air pressure is set to 0.3 to 0.5 MPa, and cultured at a temperature of 23 to 28° C. for 3 to 5 days to obtain a second-level solid bacteria;

[0043] The second liquid culture medium is prepared according to 80% of the total volume of the large fermentation tank, and the prepared second liquid culture medium is placed in the large fermentation tank. The second liquid culture medium is sterilized by steam at high temperature, the sterilization temperature is set to 121-123° C., the sterilization time is set to 30-50 minutes, and after the sterilization is completed, the temperature is lowered to 25-28° C. to obtain a sterilized second liquid culture medium.

[0044] In this embodiment, the first liquid culture medium and the second liquid culture medium have the same ingredients, and the first liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water are 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts respectively. In this embodiment, the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water in the first liquid culture medium are 0.4 parts, 4 parts, 0.05 parts, 0.05 parts and 126 parts respectively.

[0045] Specifically, the second liquid culture medium includes: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate and magnesium sulfate are 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts respectively. In this embodiment, the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water in the second liquid culture medium are 0.4 parts, 4 parts, 0.05 parts, 0.05 parts and 126 parts respectively.

[0046] After sterilizing the delivery pipeline connecting the solid strain fermentation vessel and the large fermentation tank, according to the mass ratio of the secondary solid strain to the second liquid strain culture medium of 1: (10-15), a part of the second liquid strain culture medium is reversely pressed from the large fermentation tank into the solid strain fermentation vessel, the secondary solid strain is diluted, the machine is turned on and stirred evenly, and then pressed into the large fermentation tank, mixed with the second liquid strain culture medium and inoculated, and the tertiary liquid strain production is carried out.

[0047] The temperature of the large fermentation tank is set at 28°C and the tank pressure is set at 0.05Mpa. During the fermentation, the fan is used for stirring, and the speed of the fan is 100rpm. The air valve is opened for ventilation, and the ventilation volume is 0.7m 3 / h, after 43h~46h, the dissolved oxygen content decreases, and the ventilation volume is adjusted to 1m 3 / h~1.2m 3 / h. Cultivate continuously for 24 hours to obtain the third-level liquid strain, that is, the strain of liquid white rot fungus WH-B-X3 for production.

[0048] 100 mL of the strain of liquid white rot fungus WH-B-X3 for production was taken and placed in a conical flask, and its pH value was measured using a pH meter. The pH value of the strain of liquid white rot fungus WH-B-X3 for production provided in this embodiment was 6.0. When the pH value was 5.0-7.0, it indicated that the strain of liquid white rot fungus WH-B-X3 for production was in good growth state.

[0049] The straw is crushed to 2-10 cm to obtain the third straw, and the third straw is soaked for 3 hours, and the moisture content of the soaked third straw is 55-65%. Note that the third straw does not need to be sterilized.

[0050] In this embodiment, a spraying, crushing, infiltration and dust removal unit for agricultural organic waste provided by Patent No. ZL202221165864.5 is used to pre-treat the third straw directly under the forest to be planted. In addition, the third straw can also be crushed directly by hand.

[0051] The liquid white rot fungus WH-B-X3 for production is sprayed onto the third straw at an inoculation rate of 15% to 20%, and is stirred evenly by hand or with a loader.

[0052] In this embodiment, a liquid strain mobile tank is used to transport the liquid strain to an open space under the forest, an air compressor is used to press the liquid strain out through a hose connecting the seed tank and the spray gun, or a small water pump is used to pump it out and spray it on the third straw, and a loader combined with manual turning is used to ensure that the third straw is evenly inoculated.

[0053] Specifically, the surface of the third straw with light is covered with a sunshade net, or the surface of the third straw with light is covered with at least one of 1-3 cm thick fine soil, branches and grass (weeds). In this embodiment, the third straw is covered with 1-2 cm thick fine soil.

[0054] like Figure 1 and Figure 2 As shown, the method also includes using a ventilation device to cool down the inoculated and fermented third straw a, which is suitable for the case where the length of the third straw a exceeds 2m. The ventilation device includes: a first ventilation pipe 1 and multiple second ventilation pipes 2, multiple second ventilation pipes 2 are arranged in parallel at intervals, and multiple second ventilation pipes 2 are vertically installed on the first ventilation pipe 1, and multiple ventilation holes 3 are opened on the first ventilation pipe 1 and the second ventilation pipe 2. The first ventilation pipe 1 is arranged at the bottom of the inoculated third straw a, and both ends of the first ventilation pipe 1 and the top of the second ventilation pipe 2 extend out of the third straw a. The third straw a can be covered with 1 to 2 cm thick fine soil c, and the top of the second ventilation pipe 2 extends out of the fine soil c. When implemented, the first ventilation pipe 1 and the multiple second ventilation pipes 2 can be plastic ventilation pipes. In order to facilitate installation, multiple three-way pipes can be set on the first ventilation pipe 1 for installing multiple second ventilation pipes 2. In actual use, the ventilation device can match the length and area of ​​the ridge. A large amount of hot air will be generated during the fermentation process of the third straw a. The hot air enters the ventilation device through the multiple ventilation holes 3 of the first ventilation pipe 1 and the second ventilation pipe 2. The hot air rises, moves from the first ventilation pipe 1 and the multiple second ventilation pipes 2 through the ventilation holes 3 into the ventilation device, and is discharged from the top of the second ventilation pipe 2 (pay attention to not blocking both ends of the first ventilation pipe and the top of the second ventilation pipe 2 during use), thereby realizing the circulation of hot and cold air; at the same time, during the rising process of the hot air, cold air will be inhaled from the two ends of the first ventilation pipe 1 and the third straw a (the third straw a has a certain air permeability), and the inhaled cold air can dissipate heat and cool down the fermentation process, and the oxygen carried provides the required oxygen for the rapid germination of mycelium in the normal temperature aerobic fermentation process, thereby playing a role in ventilation and oxygen supply.

[0055] like Figure 1 and Figure 2As shown, the inoculated third straw a is laid on the soil under the forest into ridges with a ridge height of 20 to 40 cm. The length of the first ventilation pipe 1 can be cut according to different needs, but the length of the first ventilation pipe 1 needs to exceed the width of each ridge, and the height of the second ventilation pipe 2 needs to exceed the thickness of the inoculated third straw a and its covering; the spacing between adjacent second ventilation pipes 2 can be 40 to 50 cm. The outer diameters of the first ventilation pipe 1 and the plurality of second ventilation pipes 2 can be 5 to 7 cm, and the aperture of the ventilation hole 3 can be 0.5 to 0.6 cm. The plurality of ventilation holes 3 can be arranged around the first ventilation pipe 1 and the plurality of second ventilation pipes 2 for ventilation, oxygen supply and heat dissipation.

[0056] The entire fermentation process is carried out under the forest. The heat generated by the fermentation of the third straw causes the air to expand and rise due to heat. The heat is discharged through the unique physical structure of the ventilation device, and cold air is driven into the inoculated and fermented third straw. This cycle is repeated to ventilate, dissipate heat and provide oxygen for the normal temperature aerobic fermentation of white rot fungi. Avoiding heat dissipation and oxygen supply through turning over and throwing, thereby avoiding the interruption of the normal temperature aerobic fermentation process by human intervention, thereby shortening the fermentation time, ensuring the continuity of fermentation, and avoiding the problem of infection caused by miscellaneous bacteria due to turning over and throwing to dissipate heat. On the 8th day of inoculation of the third straw, the hyphae of the white rot fungi can grow and cover the third straw, shortening the entire fermentation time to 18 to 30 days.

[0057] Specifically, the first straw and the second straw are steam sterilized using an herbal crushed material high temperature steam sterilizer provided by application number 202021737630.4, with a sterilization temperature of 121 to 123°C and a sterilization time of 18 to 25 minutes.

[0058] Specifically, the first straw, the second straw and the third straw can all be at least one of wheat straw, peanut straw, rice straw, corn straw, potato straw, rape straw, cotton straw and sugarcane straw. In this embodiment, the first straw, the second straw and the third straw are all mixed corn straw, wheat straw and rape straw. In this embodiment, the third straw is a mixed straw of partially moldy corn straw, wheat straw and rape straw.

[0059] Specifically, the temperature under the forest is 12 to 35°C.

[0060] Specifically, the humidity under the forest is 60-90%.

[0061] Furthermore, when the humidity under the forest is lower than 65%, the humidity is increased by watering or spraying the third straw.

[0062] Specifically, the thickness of the third straw is 20 to 40 cm. When implemented, the surface of the third straw can be covered with 1 to 2 cm thick fine soil, which can be obtained locally. Suitable fine soil near the soil under the forest is selected to avoid light, infection and water evaporation. This is more conducive to the germination of white rot fungi WH-B-X3, solid fermentation, and more conducive to promoting the fruiting of white rot fungi WH-B-X3. Usually, the fruiting can be achieved in 25 to 30 days. Figure 3 As shown, 4,000 kilograms of mushrooms can be harvested per mu. After the mushrooms are harvested, the third straw becomes a high-quality multifunctional biological organic fertilizer, such as Figure 4 As shown. Figure 4 It can be seen that the lignin and cellulose in the third straw have been decomposed by white rot fungi.

[0063] White rot fungi use straw as food during the fermentation process. While decomposing the straw, the fungus population reproduces explosively and rapidly. The number of effective live bacteria per gram of the fermented biological organic fertilizer is greater than or equal to 147 million / g (test basis is GB20287-2006), and the mass fraction of organic matter is 71.8%; on a dry basis, the mass fraction of nitrogen is 0.98%, the mass fraction of phosphorus is 1.77%, the mass fraction of potassium is 1.82%, and the mass fraction of humic acid is 27%.

[0064] The white rot fungus WH-B-X3 provided in this embodiment belongs to the genus Xylella fastidiosa, which is collected from rotten wood in the Shennongjia primeval forest, and is purified and optimized. It is repeatedly cultivated and purified against infection by miscellaneous bacteria on both non-moldy and moldy straws to obtain the white rot fungus WH-B-X3 provided in the embodiment of the present invention. The white rot fungus WH-B-X3 can be directly inoculated on the third straw that has not been sterilized for cultivation. The white rot fungus WH-B-X3 attaches to the third straw and grows in good condition. It can be seen that the white rot fungus WH-B-X3 provided by the embodiment of the present invention has excellent anti-bacterial ability, and the white rot fungus WH-B-X3 is non-toxic and edible, has a fast germination speed, and has a strong lignin decomposition ability; when the white rot fungus WH-B-X3 is used to produce biological organic fertilizer, the straw can be locally sourced, the straw is directly crushed and sprayed in the field to obtain the third straw, and then the third-level liquid strain is inoculated on the unsterilized third straw and placed under the forest for aerobic fermentation, so as to realize the preparation of biological organic fertilizer Its production process has no sewage and harmful gas emissions, is simple to operate, and has low cost. It can reduce the fertilizer input of fruit farmers and has universal applicability. In the process of fermentation of the three-level liquid bacteria, the lignin of the third straw is continuously decomposed and can be taken as needed. Its production cycle is short and the effect is quick. At the same time, the oyster mushroom can be produced after 25 days. After the white rot fungus WH-B-X3 is produced and mature, it can be harvested. The yield of the oyster mushroom per mu can reach 4,000 catties. The produced oyster mushrooms are non-toxic and edible, and can be sold after harvesting, which can increase farmers' income. After the mushrooms are grown, the lignin and cellulose in the third straw are fully decomposed, making the biological organic fertilizer rich in organic matter, nitrogen, phosphorus, potassium, active bacteria and a large amount of humic acid, providing high-quality multifunctional biological organic fertilizer for fruit trees, which can improve fruit yield and quality; the third straw after the mushrooms are grown is plowed into the ground. Under the action of active bacteria and humic acid, it can adsorb, separate and passivate heavy metals in the soil, and can also adsorb and decompose plastic particles and pesticide residues in the soil to improve soil properties. At the same time, the hyphae of the white rot fungus WH-B-X3 will release organic chemicals such as 3-octanone, which has a good killing effect on pests such as nematodes, and can improve fruit yield and quality. At the same time, the third straw is laid in the soil under the forest, which can effectively inhibit the growth of weeds in the soil under the forest, and can also achieve the effect of moisture conservation.

[0065] Embodiment 2

[0066] The difference between this embodiment and the first embodiment is that the embodiment of the present invention adopts a primary liquid culture medium, specifically comprising: a first liquid culture medium is contained in a triangular flask, the white rot fungus WH-B-X3 cultured on a plate is inoculated into the first liquid culture medium, and is shaken and cultured at a temperature of 25 to 30° C. and a rotation speed of 50 to 250 rpm for 5 to 9 days to obtain a primary liquid culture medium for standby use.

[0067] In this embodiment, the weight portions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water in the first liquid culture medium are 0.3 parts, 3 parts, 0.06 parts, 0.06 parts and 119.7 parts respectively.

[0068] In this embodiment, the weight proportions of soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water in the second liquid culture medium are 0.4 parts, 4 parts, 0.55 parts, 0.55 parts and 120 parts respectively.

[0069] Specifically, the temperature under the forest is 12-30° C. In this embodiment, the third straw is a mixture of mildew-free corn straw, wheat straw, rape straw and peanut straw, and is sterilized for use. When implemented, the surface of the third straw is covered with 1-2 cm thick fine soil.

[0070] The remaining steps are the same as those in Example 1. The experimental results of this example are the same as those in Example 1, which shows that whether the third straw is sterilized does not affect the experimental results. The third straw is not sterilized, which can reduce the fertilizer input of fruit farmers, simplify operations, and reduce costs.

[0071] Embodiment 3

[0072] The difference between this embodiment and the first embodiment is that the strain of white rot fungus WH-B-X3 and the bacillus associated with the white rot fungus WH-B-X3 are cultured together in the first straw to obtain a primary solid strain;

[0073] Specifically, the temperature under the forest is 12-30° C. In this embodiment, the third straw is a mixture of corn straw, wheat straw, rape straw and peanut straw that is not moldy. When implemented, the surface of the third straw is covered with 1-2 cm thick fine soil.

[0074] The remaining steps are the same as those in the first embodiment.

[0075] White rot fungi and bacillus use straw as food during the fermentation process. The bacillus is a companion bacteria extracted and cultivated from the infected third-level liquid strain during the large-scale production of liquid strains. While decomposing the straw, they can promote each other, so that the bacterial community can be rapidly multiplied in an explosive manner, thereby further shortening the fermentation cycle. The number of effective live bacteria per gram is greater than or equal to 752 million / g (testing basis is GB 20287-2006). In this embodiment, except that the number of effective live bacteria per gram is significantly improved compared with that of Example 1, other experimental results are the same as those of Example 1. It can be seen that whether the third straw is moldy does not affect the experimental results, and the use of moldy third straw can expand the scope of application of the method provided in this embodiment.

[0076] The above description is only an optional embodiment of the present disclosure and is not intended to limit the present disclosure. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure shall be included in the protection scope of the present disclosure.

Claims

1. A method for producing bio-organic fertilizer under forests, characterized in that: The method comprises: Cultivating a strain of white rot fungus WH-B-X3 in the first straw, wherein the white rot fungus WH-B-X3 was deposited in the China Center for Type Culture Collection on May 8, 2024, with a deposit address of Wuhan University, Wuhan, China, and a deposit number of CCTCC NO: M2024884, to obtain a primary solid strain; or inoculating the white rot fungus WH-B-X3 into the first liquid culture medium to obtain a primary liquid culture medium; Inoculating the first-level solid bacterial strain or the first-level liquid bacterial strain on the second straw for cultivation to obtain a second-level solid bacterial strain; Adding the secondary solid bacterial strain into the second liquid bacterial strain culture medium in the large fermentation tank for cultivation to obtain a tertiary liquid bacterial strain; The third-level liquid bacteria are inoculated on the third straw, and the inoculated third straw is laid on the soil under the forest for fermentation to obtain the biological organic fertilizer.

2. The method according to claim 1, characterized in that The surface of the third straw after inoculation in the light is covered with at least one of a sunshade net, soil, branches and grass.

3. The method according to claim 1, characterized in that The method also includes pre-processing the first straw, the second straw and the third straw, crushing the first straw and the second straw to 40-60 mesh, crushing the third straw to 2-10 cm, and then spraying and soaking to make the moisture content of the first straw, the second straw and the third straw 55-67%.

4. The method according to claim 1, characterized in that: The method also includes using a ventilation device to cool down and supply oxygen to the fermenting third straw, the ventilation device includes: a first ventilation pipe and a plurality of second ventilation pipes, the plurality of second ventilation pipes are arranged at intervals, and the plurality of second ventilation pipes are vertically installed on the first ventilation pipe, the first ventilation pipe and the second ventilation pipe are both provided with a plurality of ventilation holes, the first ventilation pipe is arranged between the inoculated third straw and the soil under the forest, and both ends of the first ventilation pipe and the top of the second ventilation pipe extend out of the third straw.

5. The method according to claim 1, characterized in that The temperature under the forest is 12-35°C.

6. The method according to claim 1, characterized in that The humidity under the forest is 60-90%.

7. The method according to claim 1, characterized in that When the humidity under the forest is lower than 65%, the humidity is increased by watering or spraying the third straws.

8. The method according to claim 1, characterized in that The inoculated third straw is laid on the soil under the forest to form ridges, and the ridge height is 20 to 40 cm.

9. The method according to claim 1, characterized in that: The liquid bacterial culture medium comprises: soybean meal powder, molasses, potassium dihydrogen phosphate, magnesium sulfate and water, and the weight portions of the soybean meal powder, the molasses, the potassium dihydrogen phosphate, the magnesium sulfate and the water are respectively: 0.3-0.5 parts, 3-5 parts, 0.05-0.06 parts, 0.05-0.06 parts and 119.7-126 parts.

10. The method according to claim 1, characterized in that Cultivating the white rot fungus WH-B-X3 and the Bacillus associated with the white rot fungus WH-B-X3 together in the first straw to obtain a primary solid fungus; Or the strain of the white rot fungus WH-B-X3 and the Bacillus associated with the white rot fungus WH-B-X3 are inoculated into a liquid strain culture medium to obtain a primary liquid strain.

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