Fresh rehmannia root extract, fresh rehmannia root phenolic acid components, extraction method and application thereof

Abstract

The invention discloses a fresh rehmannia extract, fresh rehmannia glutinosa phenolic acid components, an extraction method and application, and belongs to the field of daily chemical products. The extraction method comprises the following steps: adding ethanol and enzyme to fresh rehmannia glutinosa, extracting twice, and filtering to obtain a filtrate; decompressing and concentrating the filtrate, and then purifying it through a membrane, collecting the clear liquid, and obtaining a fresh rehmannia extract; separating and purifying the fresh rehmannia extract with a macroporous adsorption resin, and obtaining fresh rehmannia glutinosa phenolic acid components. The fresh rehmannia extract and fresh rehmannia glutinosa phenolic acid components extracted by the invention have good antibacterial activity against Staphylococcus aureus and Propionibacterium acnes that cause acne, and also have a significant inhibitory effect on acne muscle inflammation. The invention provides a new natural raw material and method for acne removal and anti-inflammation.

Description

Technical Field

[0001] The invention relates to the field of daily chemical products, and in particular to a fresh rehmannia root extract, fresh rehmannia root phenolic acid components, an extraction method and applications. Background Art

[0002] Acne is medically known as acne, which is generally manifested as acne, papules, pustules, nodules, cysts and scars, etc., and is accompanied by seborrhea. It is a common chronic inflammation of the hair follicle sebaceous glands in adolescence, and the main onset population is adolescent men and women. The onset is mainly related to the patient's hormone level, the secretion of skin appendages and the infection of pathogens (Propionibacterium acnes, Staphylococcus aureus and Malassezia). Among them, Staphylococcus aureus and Propionibacterium acnes are important causes of acne, and their excessive reproduction can stimulate monocytes to secrete a variety of inflammatory factors, thereby aggravating skin inflammation. Therefore, it can be considered that inhibiting the growth of Staphylococcus aureus and Propionibacterium acnes and regulating the inflammatory response induced by these two bacteria are the key links in treating acne.

[0003] Since acne often recurs, long-term use of antibiotics or hormone drugs to treat acne is prone to adverse consequences such as bacterial resistance and drug dependence. Therefore, green and functional cosmetics are becoming more and more popular, and plant extracts are being used more and more widely in cosmetics, with more and more varieties of applications.

[0004] Therefore, how to provide a plant extract with acne-removing and anti-inflammatory effects is a problem that those skilled in the art need to solve urgently. Summary of the invention

[0005] The purpose of the present invention is to provide a fresh Rehmannia root extract, fresh Rehmannia root phenolic acid components, an extraction method and application, so as to solve the problems existing in the above-mentioned prior art. The phenolic acid components extracted from fresh Rehmannia root as a raw material are green and safe extracts. The components have good antibacterial activity against Staphylococcus aureus and Propionibacterium acnes that cause acne, and can also significantly relieve the inflammatory response of acne skin. The extract can be used as a new natural raw material for acne removal and anti-inflammatory.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a method for extracting a fresh Rehmannia root extract, comprising the following steps:

[0008] Add ethanol and enzyme to fresh Rehmannia root, extract twice, and filter to obtain filtrate;

[0009] The filtrate was concentrated under reduced pressure and then purified by membrane, and the clear liquid was collected to obtain the fresh Rehmannia root extract.

[0010] Preferably, the method for obtaining the filtrate comprises the following steps:

[0011] After adding ethanol to fresh Rehmannia root at a solid-liquid ratio of 1: (5-10), 0.1%-1% of enzyme by mass was added, and extraction was performed at 45-65°C for 2-3 hours, and the filtrate and filter cake were collected by suction;

[0012] The filter cake is extracted once more in the same manner, the filtrate is collected by suction filtration, and the filtrates collected twice are combined to obtain the product.

[0013] Preferably, the enzyme comprises at least one of alkaline protease, papain and bromelain.

[0014] Preferably, the filtrate is concentrated under reduced pressure to 40%-80% of the original volume and then subjected to membrane purification treatment.

[0015] Preferably, the filtration membrane used for the membrane purification treatment includes one or more combinations of ceramic membranes, microfiltration membranes, and ultrafiltration membranes.

[0016] Preferably, the pore size of the filter membrane is 200-500 nm.

[0017] The present invention also provides a method for extracting phenolic acid components from fresh Rehmannia glutinosa, comprising the following steps:

[0018] The fresh rehmannia root extract is obtained by utilizing the extraction method, and then the fresh rehmannia root extract is separated and purified by utilizing a macroporous adsorption resin to obtain the fresh rehmannia root phenolic acid components.

[0019] Preferably, the conditions for separation and purification by the macroporous adsorption resin are as follows: 1 BV of the macroporous adsorption resin adsorbs 3-4 BV of the fresh Rehmannia glutinosa extract at a flow rate of 1.5 BV / h, and 5-7 BV of water are used for impurity removal at a flow rate of 2 BV / h.

[0020] Preferably, after removing impurities with water, elution is performed with 30%-50% ethanol aqueous solution, the elution volume is 3-5 BV, the flow rate is 1.5 BV / h, and the eluate is collected and concentrated under reduced pressure to 40%-80% of the original volume to obtain the fresh Rehmannia glutinosa phenolic acid components.

[0021] The present invention also provides the use of the fresh Rehmannia extract obtained by the extraction method or the fresh Rehmannia glutinosa phenolic acid components obtained by the extraction method in any of the following items:

[0022] (1) Application in the preparation of acne-removing and anti-inflammatory drugs and / or cosmetics;

[0023] (2) Use in the preparation of drugs and / or cosmetics for inhibiting Staphylococcus aureus;

[0024] (3) Use in the preparation of drugs and / or cosmetics for inhibiting Propionibacterium acnes;

[0025] Wherein, the amount of the fresh Rehmannia root extract or the fresh Rehmannia root phenolic acid components added to the cosmetics is 0.5%-5%.

[0026] The cosmetics involved in the present invention include but are not limited to toners, essences, gels, lotions, ointments and creams.

[0027] The present invention discloses the following technical effects:

[0028] (1) Fresh Rehmannia root is an edible Chinese medicinal material with the advantages of being green and safe. The phenolic acid components extracted from fresh Rehmannia root as a raw material in the present invention are pure plant functional raw materials. When added to various types of cosmetic formulas, the properties are stable and the application range is wide, thus providing raw materials for cosmetics.

[0029] (2) The present invention is the first to add one or a combination of alkaline protease, papain and bromelain during the extraction of fresh Rehmannia root to improve the extraction rate of fresh Rehmannia root.

[0030] (3) The present invention is the first to use macroporous resin to separate and purify the phenolic acid components of fresh Rehmannia glutinosa, which can significantly inhibit Staphylococcus aureus and Propionibacterium acnes, indicating that it has the potential to be used in cosmetics to improve acne-prone skin.

[0031] (4) The present invention first discovered that fresh Rehmannia root extract and its phenolic acid components have good antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. The anti-acne cream prepared using these ingredients as effective raw materials has significant efficacy and can effectively relieve inflammatory symptoms such as papules and pustules of acne-prone skin. Rehmannia root extract can play a good control role in the early stage of acne, while phenolic acid extract has a good control effect in the later stage of acne growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 The present invention is a process flow chart of the method for extracting phenolic acid components from fresh Rehmannia glutinosa. DETAILED DESCRIPTION

[0034] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0035] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0036] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0037] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0038] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0039] The present invention uses fresh Rehmannia root as raw material, and can quickly extract phenolic acid components of fresh Rehmannia root through alcohol extraction, enzymolysis and macroporous adsorption resin treatment. The specific process is shown in Figure 1 The extraction method is described in detail below.

[0040] Example 1 A method for extracting fresh Rehmannia root extract and phenolic acid components

[0041] (1) Use fresh Rehmannia root as raw material, wash it, cut it into pieces and crush it;

[0042] (2) After adding ethanol-water solution at a solid-liquid ratio of 1:10 (g:mL), add 1% papain (measured according to the weight of fresh Rehmannia glutinosa), extract at 65°C for 3 h, and collect the filtrate and filter cake respectively after filtration;

[0043] (3) Add the solvent and enzyme in step (2) to the filter cake, extract at 65°C for 3 h, collect the filtrate after suction filtration, and combine the two filtrates;

[0044] (4) The filtrate was concentrated to 80% of its original volume under reduced pressure and then passed through a 500 nm ceramic membrane to collect the clear liquid to obtain the fresh Rehmannia root extract with an extraction rate of 20.16%. The polyphenol and polysaccharide contents of the extract were detected using the Folin-Ciocalteu method and the phenol-sulfuric acid method. The polyphenol content accounted for 30.56 mg / L and the polysaccharide content was 70.21 mg / L.

[0045] (5) Add the clear solution from step (4) to the macroporous adsorption resin XAD-2. 1 BV of the resin can adsorb 5 BV of the clear solution at a flow rate of 1.5 BV / h. Use 7 BV of water to remove impurities at a flow rate of 2 BV / h.

[0046] (6) The product was eluted with 50% ethanol-water solution at a volume of 5 BV and a flow rate of 1.5 BV / h. The eluate was collected and concentrated under reduced pressure to 40% to 80% of the original volume to obtain phenolic acid components of fresh Rehmannia glutinosa. The content of polyphenols in the phenolic acid components was 60.82 mg / L (the polyphenol content was detected using the Folin-Ciocalteu method).

[0047] The chemical composition of the fresh Rehmannia glutinosa phenolic acid components separated above was analyzed as follows:

[0048] 1. Instruments and reagents, see Table 1.

[0049] Table 1 Instruments and reagents

[0050]

[0051] 2. Sample pretreatment method

[0052] Weigh about 30 mg of phenolic acid extract and add 400 μL of 75% methanol, vortex for 60 seconds, and sonicate on ice for 10 minutes. Centrifuge at 17,000 g for 15 minutes and take the supernatant for later use.

[0053] 3. Instrument parameter settings

[0054] Mobile phase conditions: column temperature 40°C, sample volume 1 μL; positive ion mode: A: 0.1% formic acid aqueous solution; B: acetonitrile.

[0055] Mobile phase elution gradient, see Table 2:

[0056] Table 2 Elution conditions

[0057]

[0058] Mass spectrometry conditions: AB 4500 mass spectrometer was used in MRM mode to collect primary and secondary mass spectrometry data in positive mode. The ESI ion source parameters were set as follows: ion source drying gas temperature (Gas Temp): 500°C, curtain gas (CurtainGas): 25 Psi, collision gas (Collision Gas): 10 Psi, spray voltage (IonSpray Voltage): 4500 V, and atomization temperature (Temperature): 500°C.

[0059] 4. Results Analysis

[0060] The results of LC-MS detection are shown in Table 3. The results show that the extract contains the following 8 phenolic substances, which once again confirms that the extracted phenolic substances are phenolic acid components.

[0061] Table 3 Analysis results of phenolic substances contained in the extracts

[0062]

[0063] Example 2 A method for extracting fresh Rehmannia glutinosa extract and phenolic acid components

[0064] (1) Use fresh Rehmannia root as raw material, wash it, cut it into pieces and crush it;

[0065] (2) Deionized water was added at a solid-liquid ratio of 1:5 (g:mL) and then 0.1% alkaline protease was added. The mixture was extracted at 45°C for 2 h. The filtrate and filter cake were collected after filtration.

[0066] (3) Add the solvent and enzyme in step (2) to the filter cake, extract at 45°C for 2 h, collect the filtrate after suction filtration, and combine the two filtrates.

[0067] (4) The filtrate was concentrated to 80% of its original volume under reduced pressure and then passed through a 200 nm ceramic membrane to collect the clear liquid to obtain the fresh Rehmannia root extract with an extraction rate of 17.75%. The polyphenol and polysaccharide contents of the extract were detected using the Folin-Ciocalteu method and the phenol-sulfuric acid method. The polyphenol content accounted for 21.31 mg / L and the polysaccharide content was 65.37 mg / L.

[0068] (5) The clear liquid in step (4) was added to the macroporous adsorption resin NKA-9. 1 BV of the resin can adsorb 3 BV of the clear liquid at a flow rate of 1.5 BV / h. 5 BV of water was used for impurity removal at a flow rate of 2 BV / h.

[0069] (6) The product was eluted with 30% ethanol-water solution at a volume of 3 BV and a flow rate of 1.5 BV / h. The eluate was collected and concentrated under reduced pressure to 40% to 80% of the original volume to obtain phenolic acid components of fresh Rehmannia glutinosa. The total polyphenol content in the phenolic acid components was 42.82 mg / L.

[0070] The same method as in Example 1 was used to confirm that phenolic acid components containing multiple components were separated from the fresh Rehmannia glutinosa extract.

[0071] Example 3 A method for extracting fresh Rehmannia glutinosa extract and phenolic acid components

[0072] (1) Use fresh Rehmannia root as raw material, wash it, cut it into pieces and crush it;

[0073] (2) Deionized water was added at a solid-liquid ratio of 1:5 (g:mL) and then 0.5% bromelain was added. The mixture was extracted at 55 °C for 2.5 h. The filtrate and filter cake were collected after filtration.

[0074] (3) Add the solvent and enzyme in step (2) to the filter cake, extract at 55°C for 2.5 h, collect the filtrate after suction filtration, and combine the two filtrates.

[0075] (4) The filtrate was concentrated to 80% of its original volume under reduced pressure and then passed through a 350 nm ceramic membrane to collect the clear liquid to obtain the fresh Rehmannia root extract with an extraction yield of 18.32%.

[0076] (5) Add the clear liquid from step (4) to the macroporous adsorption resin LS-308. 1 BV of the resin can adsorb 3 BV of the clear liquid at a flow rate of 1.5 BV / h. Use 6 BV of water to remove impurities at a flow rate of 2 BV / h.

[0077] (6) The product was eluted with 40% ethanol-water solution at a volume of 4 BV and a flow rate of 1.5 BV / h. The eluate was collected and concentrated under reduced pressure to 40% to 80% of the original volume to obtain phenolic acid components of fresh Rehmannia glutinosa. The content of polyphenols in the phenolic acid components was 51.69 mg / L.

[0078] The same method as in Example 1 was used to confirm that phenolic acid components containing multiple components were separated from the fresh Rehmannia glutinosa extract.

[0079] Example 4 Antibacterial Activity Study

[0080] Staphylococcus aureus is one of the important causes of acne. The antibacterial activity of fresh Rehmannia extract and phenolic acid components against Staphylococcus aureus was determined. Staphylococcus aureus (purchased from Henan Industrial Microbiological Strain Engineering Technology Research Center) was inoculated into NA medium (ingredients: peptone 10.0 g, beef extract powder 3.0 g, sodium chloride 5.0 g, agar 15.0 g, final pH 7.3±0.2) and cultured at 37°C for 3-4 days.

[0081] Take a sterile 96-well flat-bottom microplate, add 10 μL of fresh Rehmannia glutinosa phenolic acid components and 190 μL of NA liquid culture medium to each well in row A, and mix thoroughly. Add 100 μL of NA liquid culture medium, 10 μL of fresh Rehmannia glutinosa phenolic acid components, and 90 μL of the diluted bacterial solution to each well in rows B to F, and mix well. The positive control is metronidazole, and the blank control group is deionized water. The positive control and blank control are added to the culture medium as described above. Place the 96-well culture plate in a 37°C constant humidity incubator for 16 h to observe the results, measure the absorbance with an enzyme reader (540 nm), and calculate its MIC value.

[0082] The test results showed that fresh Rehmannia glutinosa extract and its phenolic acid components had good antibacterial activity against Staphylococcus aureus. The minimum inhibitory concentration (MIC) value of fresh Rehmannia glutinosa extract reached 315 ppm; the minimum inhibitory concentration (MIC) value of fresh Rehmannia glutinosa phenolic acid components reached 257 ppm.

[0083] Example 5 Antibacterial Activity Study

[0084] Propionibacterium acnes is another important cause of acne. The antibacterial activity of fresh Rehmannia glutinosa extract and phenolic acid components against Propionibacterium acnes was determined. Propionibacterium acnes (purchased from Henan Industrial Microbial Strain Engineering Technology Research Center) was inoculated on CA-B Columbia blood agar ready-to-use plates (ingredients: 10.0 g casein pancreatic digest, 3.0 g heart pancreatic digest, 1.0 g corn starch, 5.0 g meat pancreatic digest, 5.0 g yeast extract powder, 5.0 g sodium chloride, 20.0 g agar, 1 L distilled water, final pH 7.3±0.2. After sterilization at 121℃ for 15 min, it was cooled to 55℃ and then 5% sterile defibrinated sheep blood was added) and cultured at 37℃ under anaerobic conditions for 3-4 days.

[0085] Take a sterile 96-well flat-bottom microplate, add 10 μL of fresh rehmannia phenolic acid components and 180 μL of Columbia blood agar liquid culture medium to each well in row A, and mix thoroughly. Add 100 μL of sterilized liquid culture medium (17.0 g tryptone, 3.0 g soytone, 5.0 g sodium chloride, 2.5 g potassium dihydrogen phosphate, 2.5 g glucose, 1L distilled water, final pH 7.3±0.2) to each well in rows B to F, 10 μL of fresh rehmannia phenolic acid components, and 10 μL of the diluted bacterial solution in sequence, and mix well. The positive control is penicillin, and the blank control group is deionized water. The positive control and blank control are added to the culture medium in the above manner, and finally 10 μL of liquid paraffin is used to isolate the air. The 96-well culture plate is placed at 37°C under anaerobic conditions for 20 h to observe the results, and the absorbance is measured with an enzyme reader (540 nm) to calculate the MIC value.

[0086] The test results showed that fresh Rehmannia glutinosa extract and fresh Rehmannia glutinosa phenolic acid components had good antibacterial activity against Propionibacterium acnes. The minimum inhibitory concentration (MIC) value of fresh Rehmannia glutinosa extract reached 345 ppm; the minimum inhibitory concentration (MIC) value of fresh Rehmannia glutinosa phenolic acid components reached 283 ppm.

[0087] Example 6 Anti-inflammatory Activity Study

[0088] This study was based on the LPS (lipopolysaccharide)-induced RAW264.7 macrophage inflammation model to explore the anti-inflammatory activity of fresh Rehmannia glutinosa extract and its phenolic acid components.

[0089] Cell culture: The cells were cultured in a high-glucose DMEM complete culture medium (containing 100 U / mL penicillin and streptomycin) containing 10% fetal bovine serum in a 5% CO2, 37°C constant temperature incubator. RAW264.7 mouse macrophages in the logarithmic growth phase were used for the experiment.

[0090] Determination of the effects of fresh Rehmannia glutinosa extract and fresh Rehmannia glutinosa phenolic acid components on cell survival rate: The two substances were applied to RAW264.7 cells at different concentrations (0.03125 mg / mL, 0.0625 mg / mL, 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL), and the CCK-8 kit was used to detect their effects on cell survival rate.

[0091] Determine the inhibitory effect of fresh Rehmannia extract and phenolic acid components on the release of NO, IL-1β and TNF-α from RAW264.7 cells induced by LPS: Take the RAW264.7 cell suspension in the logarithmic growth phase and adjust the cell density to 2×10 5 / mL, inoculated in 96-well plates, 100 μL in each well. After 4 h of attachment, 100 μL of each of the two substances with concentrations of 50, 100, and 200 μg / mL and 25 μL of 1 mg / L LPS were added for incubation. A blank group, an LPS group, and an LPS+drug group were set, with 3 replicates in each group. After 24 h of culture, the NO content in the supernatant was detected by Griess method, and the secretion of IL-1β and TNF-α in the supernatant was detected by ELISA kit.

[0092] As shown in Table 4, at a concentration of 0-1 mg / mL, the fresh Rehmannia glutinosa extract and the phenolic acid components of fresh Rehmannia glutinosa were non-toxic to RAW264.7 cells, that is, at a concentration of 0-1 mg / mL, the two substances were within a safe concentration range for acting on RAW264.7 cells.

[0093] Table 4 Effects of fresh Rehmannia root extract and phenolic acid components of fresh Rehmannia root on the survival rate of RAW264.7 cells at different concentrations

[0094]

[0095] Inhibitory effect of fresh Rehmannia extract and fresh Rehmannia glutinosa phenolic acid components on LPS-induced release of NO, IL-1β, and TNF-α from RAW264.7 cells Compared with the blank group, after LPS stimulation, the release of NO, IL-1β, and TNF-α in the cell supernatant was significantly increased (P<0.01). As shown in Table 5, compared with the LPS group, all concentration groups of the compound can inhibit the release of NO, IL-1β, and TNF-α in the cell supernatant, and show concentration dependence (P<0.05, P<0.01).

[0096] Table 5 Inhibitory effects of fresh Rehmannia root extract and phenolic acids on the release of NO, IL-1β and TNF-α from RAW264.7 cells induced by LPS

[0097]

[0098] The experimental results showed that the anti-inflammatory effect of fresh Rehmannia glutinosa extract and phenolic acid components may be related to the inhibition of the release of NO, IL-1β and TNF-α.

[0099] Example 7 Application of fresh Rehmannia root extract and phenolic acid components with anti-inflammatory and anti-acne effects in the preparation of essence water

[0100] Adding fresh Rehmannia root extract or fresh Rehmannia root phenolic acid components to cosmetics in an amount of 0.5%-5% can prepare cosmetics with anti-inflammatory and anti-acne effects. This embodiment takes the essence water with anti-inflammatory and anti-acne effects as an example for explanation.

[0101] The components of the soothing and anti-inflammatory cream are calculated by weight: 5 parts of fresh Rehmannia root extract or phenolic acid components, 0.03 parts of Carbo-941, 4 parts of glycerin, 0.11 parts of trehalose, 0.11 parts of betaine, 0.03 parts of sodium hyaluronate, 1 part of propylene glycol, 0.2 parts of p-hydroxyacetophenone and 89.52 parts of water.

[0102] Preparation method: Weigh a set amount of glycerin and carbo-941, grind and disperse them in a beaker, then add 10 parts of water, add other set amounts of substances after dissolving carbo-941, mix well, add water, and finally add fresh Rehmannia root extract or phenolic acid components and p-hydroxyacetophenone.

[0103] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for extracting phenolic acid components from fresh Rehmannia glutinosa, characterized in that: The following steps are involved: Obtaining a fresh Rehmannia root extract, and then separating and purifying the fresh Rehmannia root extract using a macroporous adsorption resin to obtain fresh Rehmannia root phenolic acid components; The conditions for separation and purification by the macroporous adsorption resin are as follows: 1 BV of the macroporous adsorption resin adsorbs 3-4 BV of the fresh Rehmannia glutinosa extract at a flow rate of 1.5 BV / h, and 5-7 BV of water is used for impurity removal at a flow rate of 2 BV / h; after impurity removal with water, elution is performed with an ethanol aqueous solution with a volume fraction of 30%-50%, the elution volume is 3-5 BV, and the flow rate is 1.5 BV / h, and the eluate is collected and concentrated under reduced pressure to 40% to 80% of the original volume, thereby obtaining the fresh Rehmannia glutinosa phenolic acid components; The macroporous adsorption resin is any one of macroporous adsorption resin XAD-2, macroporous adsorption resin NKA-9 and macroporous adsorption resin LS-308; The extraction method of the fresh Rehmannia root extract comprises the following steps: Add ethanol aqueous solution and enzyme to fresh Rehmannia root, extract twice, and filter to obtain filtrate; The filtrate is concentrated under reduced pressure and then purified by membrane, and the clear liquid is collected to obtain the fresh Rehmannia root extract; The method for obtaining the filtrate comprises the following steps: After adding ethanol aqueous solution to fresh Rehmannia root at a material-liquid ratio of 1: (5-10), 0.1%-1% enzyme by mass is added, extraction is performed at 45-65°C for 2-3 hours, and the filtrate and filter cake are collected by suction; The filter cake is extracted once again in the same manner, the filtrate is collected by suction filtration, and the filtrates collected twice are combined to obtain the product; The enzyme includes at least one of alkaline protease, papain and bromelain; The filtrate is concentrated under reduced pressure to 40%-80% of the original volume and then subjected to membrane purification treatment.

2. The extraction method according to claim 1, characterized in that The filtration membrane used for the membrane purification treatment includes one or more combinations of ceramic membranes, microfiltration membranes, and ultrafiltration membranes.

3. The extraction method according to claim 2, characterized in that The pore size of the filter membrane is 200-500 nm.

4. Use of the fresh Rehmannia glutinosa phenolic acid components extracted by the extraction method according to any one of claims 1 to 3 in any of the following: (1) Application in the preparation of acne-removing and anti-inflammatory drugs and / or cosmetics; (2) Use in the preparation of drugs and / or cosmetics for inhibiting Staphylococcus aureus; (3) Use in the preparation of drugs and / or cosmetics for inhibiting Propionibacterium acnes; in, The amount of the fresh Rehmannia root extract or the fresh Rehmannia root phenolic acid component added to the cosmetic is 0.5%-5%.

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