Proteomics sample pretreatment and cleaning device

Abstract

The present invention is applicable to the field of biological research technology, and provides a proteomics sample pre-treatment cleaning device, comprising: a base, on which a tank body is fixedly mounted; a cleaning tank placed in the tank body and used for cleaning proteomics samples, wherein the outer wall of the cleaning tank is in contact with the inner wall of the tank body, and the top of the cleaning tank is provided with an integrally formed outer edge portion; a top cover provided on the top of the tank body, wherein the top cover is fixed to the tank body by a buckle provided, and is used to press the cleaning tank into the tank body, and a hopper is provided on the top cover, and is used to add PBS buffer for cleaning proteomics samples into the cleaning tank; wherein a filtering and draining component is provided in the cleaning tank for draining the cleaned proteomics samples. The proteomics sample pre-treatment cleaning device provided by this scheme significantly improves the efficiency and quality of proteomics sample pre-treatment through automation, uniform cooling, efficient stirring, draining and air drying.

Description

Technical Field

[0001] The invention belongs to the technical field of biological research, and in particular relates to a proteomics sample pre-treatment cleaning device. Background Art

[0002] In proteomics research, the quality of sample pretreatment directly affects the accuracy and reliability of subsequent mass spectrometry analysis. Especially for animal tissue samples, including heart, liver, spleen, lung, kidney, muscle and other tissues, they are highly complex and contain blood components. High-abundance proteins in the blood will seriously interfere with the detection of low-abundance proteins, affecting the depth and breadth of proteomics research. In traditional methods, tissue samples are washed on ice with PBS (phosphate buffered saline) to avoid protein degradation.

[0003] However, due to the uneven cooling of PBS by ice during the washing process, the proteins in some samples were degraded during the washing process, affecting the integrity of the samples and the accuracy of the experimental results.

[0004] In addition, in the prior art, the cleaning and draining processes are mostly manual operations, which is not only inefficient, but also incomplete draining affects subsequent experimental results. Summary of the invention

[0005] In order to solve the technical problems of uneven cooling of PBS by ice, low efficiency in manual operation and incomplete drainage affecting subsequent experimental results during the cleaning process of animal tissue samples, the present invention provides a proteomics sample pretreatment cleaning device.

[0006] The present invention is implemented as follows: a proteomics sample pre-treatment cleaning device comprises: a base, on which a tank body is fixedly mounted; a cleaning tank placed in the tank body and used for cleaning proteomics samples, wherein the outer wall of the cleaning tank is in contact with the inner wall of the tank body, and the top of the cleaning tank is provided with an integrally formed outer edge portion; a top cover provided on the top of the tank body, wherein the top cover is fixed to the tank body by a buckle provided, and is used for pressing the cleaning tank into the tank body, and the top cover is provided with a hopper, and is used for adding PBS buffer for cleaning proteomics samples into the cleaning tank; wherein a filtering and draining assembly for draining the cleaned proteomics samples is provided in the cleaning tank; and a stirring mechanism used in conjunction with the filtering and draining assembly and for stirring during the cleaning process is provided on the top cover.

[0007] Preferably, the tank body includes an outer tank and an inner tank, the outer tank is fixed on the base, the inner tank is arranged in the outer tank and is integrally formed with the outer tank, and a closed liquid storage chamber is formed between the outer tank and the inner tank for storing coolant, a liquid inlet pipe connected to the liquid storage chamber is fixedly installed on one side of the outer tank, and a coolant filling bucket is fixedly installed on the top of the liquid inlet pipe, and a valve is provided on the liquid inlet pipe.

[0008] Preferably, a temperature sensor is fixedly mounted on one side of the outer tank, and a detection probe of the temperature sensor extends into the liquid storage chamber to monitor the temperature of the coolant.

[0009] Preferably, a drainage hole is provided at the bottom of the cleaning tank, and a channel opening connected to the drainage hole is opened at the bottom of the tank body, and a switch mechanism is installed in the channel opening for opening and closing the drainage hole.

[0010] Preferably, the switch mechanism includes: a mounting block arranged in the channel opening, on which a plurality of support rods fixedly connected to the inner wall of the channel opening are fixedly mounted, for supporting and fixing the mounting block; a pull rod slidably mounted on the mounting block and vertically arranged, on which a spring is slidably sleeved, and the spring is located on the top of the mounting block; a sealing plug fixedly mounted on the top of the pull rod, the sealing plug is plugged at the bottom of the drainage hole to block the drainage hole to make it sealed; a fixing ring fixedly mounted on the bottom of the pull rod, on which a pull rope is fixedly connected; a pull ring fixedly connected to the other end of the pull rope, for pulling the pull rope to lower the sealing plug and open the drainage hole; a hook fixedly mounted on one side of the base, for hanging the pull ring on the hook without having to pull the pull ring all the time.

[0011] Preferably, a same telescopic sleeve is fixedly installed between the top of the mounting block and the bottom of the sealing plug, and the spring is located in the telescopic sleeve to prevent the spring from being corroded by the discharged liquid.

[0012] Preferably, a liquid receiving hopper is fixedly installed at the bottom of the tank body, the liquid receiving hopper is located directly below the channel opening, and a drain pipe is connected to the bottom opening of the liquid receiving hopper for discharging waste liquid.

[0013] Preferably, an opening and closing mechanism for opening and closing the top cover is provided on one side of the tank body, and the opening and closing mechanism includes: a first support seat fixedly mounted on one side of the tank body; a stepper motor fixedly mounted on the top of the first support seat; a hinge seat fixedly mounted on the top of the first support seat, a horizontally arranged hinge shaft being rotatably mounted on the hinge seat, one end of the hinge shaft being fixedly connected to the output shaft of the stepper motor; and a connecting block fixedly mounted on the top cover, the connecting block being fixedly sleeved on the hinge shaft.

[0014] Preferably, a plurality of compression springs are fixedly installed on the top of the inner tank, and the outer edge of the cleaning tank is placed on the top of the plurality of compression springs, so that when the top cover is opened, the outer edge of the cleaning tank can be pushed out of the tank body by the elastic force of the compression springs, thereby facilitating the staff to hold the outer edge to take out the cleaning tank.

[0015] Preferably, the tank body and the top cover are provided with an air-drying mechanism for drying the cleaned proteomics samples to remove moisture on the surface of the proteomics samples, and the air-drying mechanism comprises: a second support seat fixedly mounted on one side of the tank body; a blower fixedly mounted on the top of the second support seat, a hose connected to the air outlet end of the blower; a vertical pipe fixedly mounted on the top cover and extending to below the top cover, the other end of the hose connected to the top of the vertical pipe; an air jet pipe fixedly mounted at the bottom end of the vertical pipe, a row of air jet holes being provided at the bottom of the air jet pipe, and both ends of the air jet pipe are in a closed state.

[0016] Preferably, the filtering and draining assembly includes: an annular block arranged directly above the cleaning tank, two symmetrically arranged supporting columns are fixedly mounted on the outer wall of the annular block, and the two supporting columns are fixedly mounted on the top of the cleaning tank; a circular plate arranged in an annular accommodating groove opened on the inner wall of the annular block, and the circular plate is rotatably connected to the inner wall of the annular accommodating groove; two electric telescopic rods hinged at the bottom of the circular plate; a rotating shaft arranged at the bottom of the circular plate, and both ends of the rotating shaft are fixedly connected to the inner wall of the cleaning tank; two filter plates rotatably sleeved on the rotating shaft, and the two filter plates are respectively hinged to the output rods of the two electric telescopic rods, and when the two filter plates are unfolded into a horizontal state, they form a circle and are adapted to the cleaning tank. When the two filter plates are unfolded into a horizontal state, they are used to drain proteomics samples.

[0017] Preferably, the stirring mechanism drives the circular plate to rotate, thereby driving the filter plate in a vertical state to rotate, and the solution in the cleaning tank and the proteomics sample are stirred through the filter plate to improve the cleaning effect. The stirring mechanism includes: a rotating drum rotatably mounted on the top cover; a gear column vertically sliding in the rotating drum, both ends of the gear column extend outside the rotating drum, and annularly distributed teeth are provided on the outer wall of the gear column and the inner wall of the rotating drum, and the gear column and the rotating drum are meshed through the teeth; a reduction motor fixedly mounted on the top of the top cover, and the output shafts of the rotating drum and the reduction motor are fixedly sleeved with bevel gears, and the two bevel gears are meshed; a first cylindrical block fixedly mounted on the top of the circular plate, a tooth groove is opened on the top of the first cylindrical block, and the bottom end of the tooth column extends into the tooth groove and meshes with the tooth groove.

[0018] Preferably, the top cover is also provided with a transmission line assembly for transmitting electric energy to the two electric telescopic rods, and the transmission line assembly includes: an L-shaped plate fixedly mounted on the top of the top cover; a second cylindrical block rotatably mounted on the L-shaped plate, the bottom of the second cylindrical block is also provided with a tooth groove, and the top end of the tooth column extends into the tooth groove on the second cylindrical block, the tooth column is meshed with the tooth groove on the second cylindrical block, and the tooth column is vertically slidably connected with the inner wall of the tooth groove on the second cylindrical block; an inverted U-shaped plate fixedly mounted on the top of the L-shaped plate; a second cylindrical block rotatably mounted on ... A conductive slip ring on the plate, the bottom of the conductive slip ring is fixedly connected to the second cylindrical block; a conductive rod and an annular conductive sheet are installed at the bottom end of the tooth column; a conductive block and an annular conductive seat are assembled on the inner wall of the bottom of the tooth groove of the first cylindrical block, the conductive rod is inserted into the slot opened at the top of the conductive block, and the annular conductive sheet is inserted into the annular conductive groove opened at the top of the annular conductive seat; wherein the bottom end rotor of the conductive slip ring is connected to the conductive rod and the annular conductive sheet through two conductive wires penetrating the tooth column, and the conductive block and the annular conductive seat are also electrically connected to the two electric telescopic rods through a plurality of conductive wires.

[0019] Preferably, a first magnet block is fixedly mounted on the bottom end of the tooth column and the bottom inner wall of the tooth groove of the first cylindrical block, and the mutually adjacent sides of the two first magnet blocks have different magnetic properties, and a second magnet block is fixedly mounted on the top end of the tooth column and the top inner wall of the tooth groove of the second cylindrical block, and the mutually adjacent sides of the two second magnet blocks have different magnetic properties, and an operating sleeve for workers to hold is fixedly mounted on the tooth column. The worker holds the operating sleeve to slide the tooth column up and down, so that the second magnet blocks can be attracted to each other, or the two first magnet blocks can be attracted to each other.

[0020] Compared with the related art, the proteomics sample pretreatment and cleaning device provided by the present invention has the following beneficial effects:

[0021] The proteomics sample pretreatment cleaning device provided by the present invention aims to solve the problems in the prior art of uneven cooling of PBS used for cleaning, protein degradation, low manual operation efficiency, and incomplete drainage affecting the experimental structure during the proteomics sample pretreatment cleaning process.

[0022] By adopting a double-layer structure of the tank body and the cleaning tank, a closed liquid storage chamber is formed inside, and uniform cooling is achieved using coolant, which effectively avoids protein degradation during the cleaning process and improves the integrity of the sample and the reliability of the experimental results.

[0023] The stirring mechanism and the filter and drain components in the device realize automatic stirring and draining, which not only greatly improves the operating efficiency, but also effectively avoids the errors introduced by human operation, ensuring the consistency of experimental conditions and the repeatability of experimental results. In particular, the stirring mechanism drives the circular plate to rotate, thereby driving the filter plate in a vertical state to rotate, stirring the solution and proteomics samples in the cleaning tank, significantly improving the cleaning effect;

[0024] At the same time, when the filter plate is unfolded to a horizontal state, it can drain the cleaned proteomics samples, and facilitate the air-drying mechanism to effectively dry the moisture on the surface of the proteomics samples, providing purer samples for subsequent experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic diagram of the front view of a proteomics sample pre-treatment and cleaning device provided by the present invention;

[0026] Figure 2 A schematic diagram of the front cross-sectional structure of a proteomics sample pre-treatment and cleaning device provided by the present invention;

[0027] Figure 3 for Figure 2 An enlarged structural diagram of part A shown in FIG.

[0028] Figure 4 for Figure 2 An enlarged schematic diagram of the structure of part B shown in FIG.

[0029] Figure 5 for Figure 2 An enlarged schematic diagram of the structure of part C shown in FIG.

[0030] Figure 6 for Figure 2 An enlarged structural diagram of part D shown in FIG.

[0031] Figure 7 for Figure 2 An enlarged structural diagram of part E shown in FIG.

[0032] Figure 8 for Figure 2 An enlarged structural diagram of part F shown in FIG.

[0033] Fig. 9 It is a top view schematic diagram of the assembly of the annular block and the support column in the present invention;

[0034] Fig.10 It is a top view disassembly schematic diagram of the filter plate of the present invention when it is unfolded;

[0035] Fig.11 It is a top view and cross-sectional assembly diagram of the filter plate and the rotating shaft when the filter plate is unfolded in the present invention;

[0036] Fig.12 It is a top view schematic diagram of the assembly of the mounting block and the support rod in the present invention;

[0037] Fig.13 It is a schematic diagram of the front cross-sectional structure of the tank body in the present invention.

[0038] Figure numerals: 1, base; 2, cleaning tank; 201, outer edge; 202, compression spring; 3, top cover; 301, feeding hopper; 4, annular block; 401, support column; 5, tank body; 6, circular plate; 7, electric telescopic rod; 8, rotating shaft; 9, filter plate; 10, rotating drum; 11, gear column; 12, reduction motor; 13, bevel gear; 14, first cylindrical block; 16, mounting block; 17, support rod; 18, pull rod; 19, spring; 20, sealing plug; 21, fixing ring; 22, pull rope; 23, pull ring; 24, hook; 25, telescopic sleeve; 26, liquid receiving bucket; 27, drainage Tube; 28. first support seat; 29. ​​stepper motor; 30. connecting block; 31. second support seat; 32. blower; 33. hose; 34. vertical pipe; 35. jet pipe; 36. L-shaped plate; 37. second cylindrical block; 38. inverted U-shaped plate; 39. conductive slip ring; 40. conductive rod; 41. annular conductive sheet; 42. conductive block; 43. annular conductive seat; 44. first magnet block; 45. second magnet block; 46. operating sleeve; 47. hinged seat; 51. outer tank; 52. inner tank; 53. liquid storage chamber; 54. liquid inlet pipe; 55. coolant filling bucket; 56. temperature sensor. DETAILED DESCRIPTION

[0039] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of this application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification of this application and the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0040] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0041] The embodiment of the present invention provides a proteomics sample pre-treatment cleaning device, such as Figure 1-13 As shown, the proteomics sample pre-treatment cleaning device comprises: a base 1, on which a tank body 5 is fixedly mounted; a cleaning tank 2 placed in the tank body 5 and used for cleaning proteomics samples, wherein the outer wall of the cleaning tank 2 is in contact with the inner wall of the tank body 5, and the top of the cleaning tank 2 is provided with an integrally formed outer edge portion 201; a top cover 3 provided on the top of the tank body 5, wherein the top cover 3 is fixed to the tank body 5 by a buckle provided, and is used for pressing the cleaning tank 2 into the tank body 5, and the top cover 3 is provided with a hopper 301, which is used for adding PBS buffer for cleaning proteomics samples into the cleaning tank 2; wherein a filtering and draining assembly for draining the cleaned proteomics samples is provided in the cleaning tank 2; and a stirring mechanism used in conjunction with the filtering and draining assembly for stirring during the cleaning process is provided on the top cover 3.

[0042] In this embodiment, the base 1 stably supports the entire device, the tank body 5 is mounted on the base 1, and the cleaning tank 2 is precisely placed in the tank body 5, ensuring the stability of the cleaning tank 2. The top cover 3 is tightly fixed to the tank body 5 by buckles, which not only ensures the stability of the cleaning tank 2, but also adds PBS buffer into the cleaning tank 2 through the hopper 301, thereby realizing automated sample cleaning.

[0043] The filtering and draining assembly in the cleaning tank 2 can effectively drain the proteomics samples after cleaning. The stirring mechanism arranged on the top cover 3 drives the filter plate 9 to stir by driving the circular plate 6 to rotate, thereby ensuring sufficient contact between the PBS buffer and the sample, improving the cleaning efficiency and uniformity, and reducing protein degradation, thereby ensuring the integrity of the sample.

[0044] In a further preferred embodiment of the present invention, the tank body 5 includes an outer tank 51 and an inner tank 52, the outer tank 51 is fixed on the base 1, the inner tank 52 is arranged in the outer tank 51 and is integrally formed with the outer tank 51, and a closed liquid storage chamber 53 is formed between the outer tank 51 and the inner tank 52 for storing coolant, a liquid inlet pipe 54 connected to the liquid storage chamber 53 is fixedly installed on one side of the outer tank 51, and a coolant filling bucket 55 is fixedly installed on the top of the liquid inlet pipe 54, and a valve is provided on the liquid inlet pipe 54.

[0045] In this embodiment, the double-layer structure design of the tank body 5, including the outer tank 51 and the inner tank 52, significantly improves the cooling effect and safety of the device. The outer tank 51 is firmly fixed on the base 1 and is integrally formed with the inner tank 52 to jointly construct a closed liquid storage chamber 53, providing a dedicated storage space for the coolant. The injection and management of the coolant are achieved through the liquid inlet pipe 54 set on one side of the outer tank 51. The coolant filling hopper 55 at the top of the liquid inlet pipe 54 facilitates the addition of coolant, and the valve on the liquid inlet pipe 54 ensures the control and safety of the coolant during use.

[0046] The cooling liquid added into the liquid storage chamber 53 can cool the cleaning tank 2, thereby cooling the PBS, effectively avoiding the degradation of protein during the cleaning process, and improving the integrity of the sample and the accuracy of the experiment.

[0047] In a further preferred embodiment of the present invention, a temperature sensor 56 is fixedly installed on one side of the outer tank 51, and a detection probe of the temperature sensor 56 extends into the liquid storage chamber 53 to monitor the temperature of the coolant.

[0048] In this embodiment, the integrated use of the temperature sensor 56 further improves the intelligence level of the device and the accuracy of the experiment. The temperature sensor 56 is fixed on one side of the outer tank 51, and its detection probe is deep inside the liquid storage chamber 53, which can monitor the temperature change of the coolant in real time, ensuring that the temperature control during the cleaning process reaches an ideal stable state. This design not only avoids protein degradation caused by temperature fluctuations, ensures the purity of the sample and the consistency of the experiment, but also provides the operator with intuitive temperature information through real-time temperature feedback, which is convenient for timely adjustment of the coolant state and optimization of the cleaning conditions, thereby significantly improving the efficiency and quality of proteomics sample pretreatment.

[0049] In a further preferred embodiment of the present invention, a drainage hole is provided at the bottom of the cleaning tank 2, and a channel opening connected to the drainage hole is opened at the bottom of the tank body 5, and a switch mechanism is installed in the channel opening for opening and closing the drainage hole.

[0050] In this embodiment, the drainage hole designed at the bottom of the cleaning tank 2 is closely connected to the channel opening at the bottom of the tank body 5, and the switch mechanism is cleverly integrated to achieve convenient drainage of the cleaning liquid. This innovative design not only simplifies the drainage step after cleaning, but also improves the operating efficiency.

[0051] In a further preferred embodiment of the present invention, the switch mechanism includes: a mounting block 16 arranged in the passage opening, on which a plurality of support rods 17 fixedly connected to the inner wall of the passage opening are fixedly mounted, for supporting and fixing the mounting block 16; a pull rod 18 slidably mounted on the mounting block 16 and arranged vertically, on which a spring 19 is slidably sleeved, and the spring 19 is located at the top of the mounting block 16; a sealing plug 20 fixedly mounted on the top of the pull rod 18, the sealing plug 20 is plugged at the bottom of the drainage hole to block the drainage hole to make it sealed; a fixing ring 21 fixedly mounted on the bottom end of the pull rod 18, on which a pull rope 22 is fixedly connected; a pull ring 23 fixedly connected to the other end of the pull rope 22, for pulling the pull rope 22 to lower the sealing plug 20 and open the drainage hole; a hook 24 fixedly mounted on one side of the base 1, for hanging the pull ring 23 on the hook 24 without pulling the pull ring all the time.

[0052] In this embodiment, the switch mechanism includes a mounting block 16 and a support rod 17 thereon for stable support. A pull rod 18 is slidably mounted on the mounting block 16, and a spring 19 is sleeved thereon to provide a reset force. A sealing plug 20 is fixed to the top of the pull rod 18 for sealing the drainage hole. The bottom of the pull rod 18 is connected to a fixing ring 21, which in turn connects a pull rope 22 and a pull ring 23 for easy operation. A hook 24 is provided on one side of the base 1 for easy fixing of the pull ring 23 for convenient operation. This design can easily control the opening and closing of the drainage hole by pulling the pull ring 23, thereby improving the convenience and efficiency of use. At the same time, the structure is compact, easy to install and maintain, and embodies significant beneficial effects. When the pull ring 23 is pulled, the pull rod 18 drives the sealing plug 20 to descend, the spring 19 is compressed, and the drainage hole is opened; when the pull ring 23 is released, the spring 19 returns to its original state, pushes the sealing plug 20 to rise, and the drainage hole is closed, thereby achieving effective control of the drainage hole.

[0053] In a further preferred embodiment of the present invention, a same telescopic sleeve 25 is fixedly installed between the top of the mounting block 16 and the bottom of the sealing plug 20, and the spring 19 is located in the telescopic sleeve 25 to prevent the spring 19 from being corroded by the discharged liquid.

[0054] In this embodiment, by fixing the telescopic sleeve 25 between the top of the mounting block 16 and the bottom of the sealing plug 20 and placing the spring 19 in the telescopic sleeve 25, the spring 19 is effectively prevented from being corroded by the discharged liquid, thereby extending the service life.

[0055] In a further preferred embodiment of the present invention, a liquid receiving hopper 26 is fixedly installed at the bottom of the tank body 5, and the liquid receiving hopper 26 is located directly below the channel opening. The bottom of the liquid receiving hopper 26 is connected to a drain pipe 27 for discharging waste liquid.

[0056] In this embodiment, the liquid receiving hopper 26 fixedly installed at the bottom of the tank body 5 is cleverly located just below the channel opening, effectively receiving the waste liquid discharged from the drain hole. The drain pipe 27 connected to the bottom of the liquid receiving hopper 26 is responsible for smoothly draining the waste liquid. Through the coordinated use of the liquid receiving hopper 26 and the drain pipe 27, the waste liquid is effectively collected and quickly discharged.

[0057] In another embodiment of the present invention, the filtering and draining assembly includes: an annular block 4 arranged directly above the cleaning tank 2, two symmetrically arranged support columns 401 are fixedly installed on the outer wall of the annular block 4, and the two support columns 401 are fixedly installed on the top of the cleaning tank 2; a circular plate 6 arranged in an annular accommodating groove opened on the inner wall of the annular block 4, and the circular plate 6 is rotatably connected to the inner wall of the annular accommodating groove; two electric telescopic rods 7 hinged at the bottom of the circular plate 6; a rotating shaft 8 arranged at the bottom of the circular plate 6, and both ends of the rotating shaft 8 are fixedly connected to the inner wall of the cleaning tank 2; two filter plates 9 rotatably sleeved on the rotating shaft 8, and the two filter plates 9 are respectively hinged to the output rods of the two electric telescopic rods 7. When the two filter plates 9 are unfolded into a horizontal state, they form a circle and are compatible with the cleaning tank 2. When the two filter plates 9 are unfolded into a horizontal state, they are used to drain proteomics samples.

[0058] In this embodiment, the design of the filter and drain assembly is ingenious. It is cleverly arranged just above the cleaning tank 2, and includes an annular block 4, a support column 401, a circular plate 6, an electric telescopic rod 7, a rotating shaft 8, and a filter plate 9. The annular block 4 is firmly mounted on the top of the cleaning tank 2 through the support column 401, and the circular plate 6 is flexibly rotated in the annular receiving groove on its inner wall. Two electric telescopic rods 7 are hinged at the bottom of the circular plate 6 and are connected to two filter plates 9 rotatably sleeved on the rotating shaft 8. When the filter plates 9 are pulled and unfolded into a horizontal state by the electric telescopic rod 7, they perfectly form a circle that is compatible with the cleaning tank 2, and can now be used for efficient drainage of proteomics samples. This design not only greatly improves the convenience and efficiency of drainage, but also ensures the integrity and quality of proteomics samples during the drainage process, showing its significant beneficial effects.

[0059] In another embodiment of the present invention, the stirring mechanism drives the circular plate 6 to rotate, thereby driving the filter plate 9 in a vertical state to rotate, and the solution and the proteomics sample in the cleaning tank 2 are stirred through the filter plate 9 to improve the cleaning effect. The stirring mechanism includes: a rotating drum 10 rotatably mounted on the top cover 3; a gear column 11 vertically sliding in the rotating drum 10, both ends of the gear column 11 extend outside the rotating drum 10, and annularly distributed teeth are provided on the outer wall of the gear column 11 and the inner wall of the rotating drum 10, and the gear column 11 and the rotating drum 10 are meshed with teeth; a reduction motor 12 fixedly mounted on the top of the top cover 3, and the output shafts of the rotating drum 10 and the reduction motor 12 are fixedly sleeved with a bevel gear 13, and the two bevel gears 13 are meshed with each other; a first cylindrical block 14 fixedly mounted on the top of the circular plate 6, a tooth groove is opened on the top of the first cylindrical block 14, and the bottom end of the gear column 11 extends into the tooth groove and meshes with the tooth groove.

[0060] In this embodiment, the design of the stirring mechanism ingeniously realizes efficient stirring of the PBS solution and proteomics samples in the cleaning tank 2 to improve the cleaning effect. The stirring mechanism includes key components such as a rotating drum 10, a tooth column 11, a reduction motor 12 and a bevel gear 13. The rotating drum 10 is rotatably mounted on the top cover 3, and the tooth column 11 slides vertically inside it and meshes with the rotating drum 10 through teeth. The reduction motor 12 is fixedly mounted on the top of the top cover 3, and the bevel gear 13 on its output shaft meshes with the bevel gear 13 on the rotating drum 10, thereby driving the rotating drum 10 and the tooth column 11 to rotate. A tooth groove is provided on the first cylindrical block 14 at the top of the circular plate 6, and the bottom end of the tooth column 11 meshes with it. Therefore, when the tooth column 11 rotates, it will drive the circular plate 6 and the filter plate 9 in a vertical state to rotate together, so as to achieve full stirring of the solution and sample in the cleaning tank 2. This design not only improves the cleaning efficiency, but also ensures that the proteomics samples are evenly stressed during the cleaning process, effectively improving the cleaning effect and demonstrating its significant beneficial effects.

[0061] In another embodiment of the present invention, an air-drying mechanism for drying the cleaned proteomics sample is provided on the tank body 5 and the top cover 3 to remove moisture on the surface of the proteomics sample, and the air-drying mechanism includes: a second support seat 31 fixedly mounted on one side of the tank body 5; a blower 32 fixedly mounted on the top of the second support seat 31, and a hose 33 is connected to the air outlet end of the blower 32; a vertical pipe 34 fixedly mounted on the top cover 3 and extending to the bottom of the top cover 3, and the other end of the hose 33 is connected to the top of the vertical pipe 34; an air jet pipe 35 fixedly mounted at the bottom end of the vertical pipe 34, and a row of air jet holes is provided at the bottom of the air jet pipe 35, and both ends of the air jet pipe 35 are in a closed state.

[0062] In actual experiments, in order to know more accurately how much tissue sample we have used, we need to weigh the tissue sample. In the prior art, in order to weigh more accurately, after washing the tissue sample and draining off the water appropriately, it is generally necessary to use filter paper to absorb the water on the surface of the sample before weighing it, which is more troublesome.

[0063] In the present embodiment, by adding an air-drying mechanism to the tank body 5 and the top cover 3, the function of drying the proteomics sample after cleaning is realized, and the surface moisture of the sample is effectively removed. The air-drying mechanism includes a second support seat 31, an air blower 32, a hose 33, a vertical pipe 34 and an air jet 35. The air blower 32 is fixedly mounted on the second support seat 31, and its air outlet end is connected to the vertical pipe 34 through a hose 33, and the vertical pipe 34 extends to the bottom of the top cover 3 and is finally connected to the air jet 35. A row of air jet holes is provided at the bottom of the air jet 35 for blowing air flow to the sample to accelerate the evaporation of water. At the same time, the reduction motor 12 drives the filter plate 9 to rotate when it is unfolded into a horizontal state, thereby driving the proteomics sample after cleaning to rotate, thereby realizing the air drying of the proteomics sample on the filter plate 9, effectively removing the surface moisture of the proteomics sample, and improving the sample surface moisture removal efficiency, without using filter paper to absorb the surface moisture of the proteomics sample.

[0064] In another embodiment of the present invention, the top cover 3 is also provided with a transmission line assembly for transmitting electric energy to the two electric telescopic rods 7, and the transmission line assembly includes: an L-shaped plate 36 fixedly mounted on the top of the top cover 3; a second cylindrical block 37 rotatably mounted on the L-shaped plate 36, the bottom of the second cylindrical block 37 is also provided with a tooth groove, and the top of the tooth column 11 extends into the tooth groove on the second cylindrical block 37, the tooth column 11 is meshed with the tooth groove on the second cylindrical block 37, and the tooth column 11 is vertically slidably connected to the inner wall of the tooth groove on the second cylindrical block 37; an inverted U-shaped plate 38 fixedly mounted on the top of the L-shaped plate 36; and a second cylindrical block 37 mounted on the inverted U-shaped plate 38. A conductive slip ring 39, the bottom of which is fixedly connected to the second cylindrical block 37; a conductive rod 40 and an annular conductive sheet 41 installed at the bottom end of the tooth column 11; a conductive block 42 and an annular conductive seat 43 mounted on the inner wall of the bottom of the tooth groove of the first cylindrical block 14, the conductive rod 40 is inserted into the slot opened at the top of the conductive block 42, and the annular conductive sheet 41 is inserted into the annular conductive groove opened at the top of the annular conductive seat 43; wherein the bottom end rotor of the conductive slip ring 39 is connected to the conductive rod 40 and the annular conductive sheet 41 through two conductive wires passing through the tooth column 11, and the conductive block 42 and the annular conductive seat 43 are also electrically connected to the two electric telescopic rods 7 through a plurality of wires.

[0065] In this embodiment, a transmission line assembly is cleverly arranged on the top cover 3 to provide stable power transmission for the two electric telescopic rods 7. The assembly includes key components such as an L-shaped plate 36, a second cylindrical block 37, an inverted U-shaped plate 38, a conductive slip ring 39, a conductive rod 40, an annular conductive sheet 41, a conductive block 42, and an annular conductive seat 43. Among them, the second cylindrical block 37 is rotatably mounted on the L-shaped plate 36 and meshes with the tooth groove at the top of the tooth column 11 to achieve synchronous rotation. The conductive slip ring 39 is fixed on the inverted U-shaped plate 38, and its bottom is connected to the second cylindrical block 37, and maintains stable power transmission as it rotates. The conductive rod 40 and the annular conductive sheet 41 are installed at the bottom of the tooth column 11, and cooperate with the conductive block 42 and the annular conductive seat 43 in the first cylindrical block 14 to form a complete circuit connection. When the tooth column 11 rotates, the conductive slip ring 39 connected to the external power supply through the conductive wire provides power to the conductive rod 40 and the annular conductive sheet 41 through the conductive wire, and then supplies power to the two electric telescopic rods 7 through the conductive block 42 and the annular conductive seat 43. This design not only ensures the stable power supply of the electric telescopic rod 7 during the rotation process, but also improves the convenience and safety of the entire device, showing its significant beneficial effects.

[0066] In another embodiment of the present invention, a first magnet block 44 is fixedly mounted on the bottom end of the tooth column 11 and the inner wall of the bottom of the tooth groove of the first cylindrical block 14, and the magnetism of the two first magnet blocks 44 that are close to each other is different, and a second magnet block 45 is fixedly mounted on the top end of the tooth column 11 and the inner wall of the top of the tooth groove of the second cylindrical block 37, and the magnetism of the two second magnet blocks 45 that are close to each other is different, and an operating sleeve 46 for workers to hold is fixedly mounted on the tooth column 11. The worker holds the operating sleeve 46 to slide the tooth column 11 up and down, so that the second magnet blocks 45 can be attracted to each other, and the two first magnet blocks 44 can be attracted to each other.

[0067] In this embodiment, in order to further improve the convenience and stability of operation, the first magnet block 44 is fixedly installed on the bottom end of the tooth column 11 and the inner wall of the bottom of the tooth groove of the first cylindrical block 14, and the magnetism of the two first magnet blocks 44 close to each other is different, so that they can attract each other and maintain a stable connection. Similarly, the second magnet block 45 is also fixedly installed on the top of the tooth column 11 and the inner wall of the top of the tooth groove of the second cylindrical block 37, and the magnetism of the two second magnet blocks 45 close to each other is also different, realizing the function of mutual attraction. In addition, an operating sleeve 46 is fixedly sleeved on the tooth column 11 for the convenience of workers to operate by hand. By holding the operating sleeve 46 to slide the tooth column 11 up and down, the mutual attraction state of the second magnet block 45 and the first magnet block 44 can be flexibly controlled, thereby realizing effective control and stable operation of the entire device. This design not only improves the convenience of operation, but also enhances the stability and reliability of the device during use, showing its significant beneficial effects.

[0068] In a further preferred embodiment of the present invention, one side of the tank body 5 is provided with an opening and closing mechanism for opening and closing the top cover 3, and the opening and closing mechanism includes: a first support seat 28 fixedly mounted on one side of the tank body 5; a stepper motor 29 fixedly mounted on the top of the first support seat 28; a hinge seat 47 fixedly mounted on the top of the first support seat 28, a horizontally arranged hinge shaft being rotatably mounted on the hinge seat 47, one end of the hinge shaft being fixedly connected to the output shaft of the stepper motor 29; a connecting block 30 fixedly mounted on the top cover 3, and the connecting block 30 being fixedly sleeved on the hinge shaft.

[0069] In this embodiment, an opening and closing mechanism is cleverly arranged on one side of the tank body 5 to conveniently control the opening and closing of the top cover 3. The opening and closing mechanism includes key components such as a first support seat 28, a stepper motor 29, a hinge seat 47, and a connecting block 30. Among them, the first support seat 28 is fixedly mounted on one side of the tank body 5, and the stepper motor 29 and the hinge seat 47 are fixedly mounted on the top thereof. A horizontally arranged hinge shaft is rotatably mounted on the hinge seat 47, and one end of the shaft is fixedly connected to the output shaft of the stepper motor 29, thereby realizing the driving function of the motor. The connecting block 30 is fixedly mounted on the top cover 3 and sleeved on the hinge shaft, so that the top cover 3 can realize the opening and closing action by the rotation of the hinge shaft. When the stepper motor 29 is started, it drives the hinge shaft to rotate, thereby driving the connecting block 30 and the top cover 3 to perform the opening and closing movement. This design not only realizes the automatic opening and closing of the top cover 3, but also improves the convenience and efficiency of operation.

[0070] In a further preferred embodiment of the present invention, a plurality of compression springs 202 are fixedly installed on the top of the inner tank 52, and the outer edge 201 of the cleaning tank 2 is placed on the top of the plurality of compression springs 202, so that when the top cover 3 is opened, the outer edge of the cleaning tank 2 is pushed to the outside of the tank body 5 by the elastic force of the compression springs 202, thereby making it convenient for the staff to hold the outer edge to take out the cleaning tank 2.

[0071] In this embodiment, a plurality of compression springs 202 are fixedly installed on the top of the inner tank 52, and the outer edge 201 of the cleaning tank 2 is cleverly placed on the top of these compression springs 202. This design enables the elastic force of the compression spring 202 to push the outer edge of the cleaning tank 2 to the outside of the tank body 5 when the top cover 3 is opened, thereby providing a convenient way for the staff to take out the cleaning tank 2. They only need to hold the outer edge to easily take the cleaning tank 2 out of the tank body 5. This design not only simplifies the tank taking process and improves work efficiency, but also reduces the labor intensity of the staff, showing its significant beneficial effects.

[0072] It is worth noting that the circuits, electronic components and modules involved in the present invention are all prior art, and those skilled in the art can fully implement them. Needless to say, the content protected by the present invention does not involve improvements to software and methods.

[0073] This solution also provides an electric control cabinet, which is installed on the equipment. When in use, each electrical equipment can be started and operated separately through the electric control cabinet. The power connection method of each electrical equipment is an existing mature technology, which is a well-known technology for people in this field and will not be elaborated here.

[0074] In summary, compared with the related art, the present invention effectively overcomes the key challenges in the proteomics sample pretreatment process in the prior art. The double-layer tank body 5 works in conjunction with the cleaning tank 2, and the internal liquid storage chamber 53 is evenly cooled by the coolant, which significantly reduces the problem of uneven cooling of PBS, thereby effectively preventing protein degradation and ensuring the integrity of the sample and the reliability of the experimental results;

[0075] In addition, the integration of the stirring mechanism and the filtering and draining component realizes the automated stirring and draining process, greatly improving the operating efficiency. The stirring mechanism drives the circular plate 6 to rotate, which not only enhances the cleaning effect, but also uniformly stirs the proteomics sample through the rotation of the filter plate 9 in the vertical state, thereby improving the cleaning effect. At the same time, it effectively drains water and assists air drying in the horizontal state, ensuring the effective removal of moisture on the sample surface, improving the processing efficiency, providing higher purity samples for subsequent experiments, and significantly improving the accuracy and reliability of the experiment.

[0076] In the several embodiments provided in this application, it should be understood that the disclosed device can be implemented in other ways.

[0077] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. A proteomics sample pretreatment cleaning device, characterized in that: include: A base (1), a tank body (5) being fixedly mounted on the base (1); A cleaning tank (2) placed in the tank body (5) and used for cleaning proteomics samples, wherein the outer wall of the cleaning tank (2) contacts the inner wall of the tank body (5), and the top of the cleaning tank (2) is provided with an integrally formed outer edge portion (201); A top cover (3) is arranged on the top of the tank body (5), the top cover (3) is fixed to the tank body (5) by means of a buckle, and is used to press the cleaning tank (2) into the tank body (5), and a hopper (301) is arranged on the top cover (3) and is used to add PBS buffer for cleaning protein samples into the cleaning tank (2); Wherein, the cleaning tank (2) is provided with a filtering and draining component for draining the protein sample after cleaning; The top cover (3) is provided with a stirring mechanism used in conjunction with the filter and drain assembly for stirring during the cleaning process; The cleaning tank (2) is provided with a drainage hole at the bottom, and the tank body (5) is provided with a channel opening connected to the drainage hole at the bottom, and a switch mechanism is installed in the channel opening for opening and closing the drainage hole; The switch mechanism comprises: a mounting block (16) arranged in the passage opening, a plurality of support rods (17) fixedly mounted on the mounting block (16) and fixedly connected to the inner wall of the passage opening, for supporting and fixing the mounting block (16); a pull rod (18) slidably mounted on the mounting block (16) and arranged vertically, a spring (19) being slidably sleeved on the pull rod (18), the spring (19) being located at the top of the mounting block (16); a sealing plug (20) fixedly mounted on the top of the pull rod (18), the sealing plug (20) is plugged at the bottom of the drainage hole to block the drainage hole and make it sealed; a fixing ring (21) is fixedly installed at the bottom end of the pull rod (18), and a pull rope (22) is fixedly connected to the fixing ring (21); a pull ring (23) is fixedly connected to the other end of the pull rope (22), and is used to pull the pull rope (22) to lower the sealing plug (20) to open the drainage hole; a hook (24) is fixedly installed on one side of the base (1), and is used to hang the pull ring (23) on the hook (24) without having to pull the pull ring all the time; An opening and closing mechanism for opening and closing the top cover (3) is provided on one side of the tank body (5), and the opening and closing mechanism comprises: a first support seat (28) fixedly mounted on one side of the tank body (5); a stepper motor (29) fixedly mounted on the top of the first support seat (28); a hinge seat (47) fixedly mounted on the top of the first support seat (28), a horizontally arranged hinge shaft being rotatably mounted on the hinge seat (47), one end of the hinge shaft being fixedly connected to an output shaft of the stepper motor (29); and a connecting block (30) fixedly mounted on the top cover (3), the connecting block (30) being fixedly sleeved on the hinge shaft.

2. The proteomics sample pretreatment and cleaning device according to claim 1, characterized in that: The tank body (5) comprises an outer tank (51) and an inner tank (52), wherein the outer tank (51) is fixed on the base (1), and the inner tank (52) is arranged inside the outer tank (51) and is formed integrally with the outer tank (51), and a closed liquid storage chamber (53) is formed between the outer tank (51) and the inner tank (52) for storing coolant, and a liquid inlet pipe (54) communicating with the liquid storage chamber (53) is fixedly mounted on one side of the outer tank (51), and a coolant filling hopper (55) is fixedly mounted on the top end of the liquid inlet pipe (54), and a valve is arranged on the liquid inlet pipe (54).

3. The proteomics sample pretreatment and cleaning device according to claim 2, characterized in that: A temperature sensor (56) is fixedly mounted on one side of the outer tank (51), and a detection probe of the temperature sensor (56) extends into the liquid storage chamber (53) for monitoring the temperature of the coolant.

4. The proteomics sample pretreatment and cleaning device according to claim 1, characterized in that: A telescopic sleeve (25) is fixedly mounted between the top of the mounting block (16) and the bottom of the sealing plug (20), and the spring (19) is located inside the telescopic sleeve (25) to prevent the spring (19) from being corroded by the discharged liquid.

5. The proteomics sample pretreatment and cleaning device according to claim 1, characterized in that: A liquid receiving hopper (26) is fixedly mounted on the bottom of the tank body (5), the liquid receiving hopper (26) being located directly below the channel opening, and a liquid discharge pipe (27) is connected to the bottom opening of the liquid receiving hopper (26) for discharging waste liquid.

6. The proteomics sample pre-treatment cleaning device according to claim 2, characterized in that: A plurality of compression springs (202) are fixedly mounted on the top of the inner tank (52), and the outer edge (201) of the cleaning tank (2) is placed on the top of the plurality of compression springs (202). When the top cover (3) is opened, the outer edge of the cleaning tank (2) is pushed out of the tank body (5) by utilizing the elastic force of the compression springs (202), thereby making it convenient for a worker to hold the outer edge to take out the cleaning tank (2).

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