Method for promoting germination of coriander seeds

Abstract

The invention belongs to the technical field of seed treatment, and particularly relates to a method for promoting germination of coriander seeds. The method for promoting the germination of the awngrass seeds comprises the following steps: before the germination of the awngrass seeds, pretreating the awngrass seeds by using a seed soaking agent; the seed soaking agent is a solution containing a plant growth regulator; the concentration of the plant growth regulator in the seed soaking agent is 5mg / L to 160mg / L. According to the method, seeds are soaked in a seed soaking agent, so that dormancy of the miscanthus coriander seeds is broken, and germination of the miscanthus coriander seeds is promoted. According to the method provided by the invention, the germination speed, the germination rate, the germination potential and the germination index of the miscanthus coriander seeds are remarkably improved. In addition, as the seed soaking agent can be used for repeatedly soaking the seeds, the method also has the characteristics of simplicity in operation, time and labor saving and low cost. Therefore, the method disclosed by the invention has important application and popularization values for the technology of breaking dormancy of the miscanthus coriander seeds and promoting germination of the miscanthus coriander seeds.

Description

Technical Field

[0001] The invention belongs to the technical field of seed treatment, and in particular relates to a method for promoting the germination of Miscanthus sinensis seeds. Background Art

[0002] Miscanthus is an annual herbaceous plant of the genus Pentatomus in the Poaceae family. It is a component of the Central Asian flora. It is distributed in the arid and semi-arid regions of Central Asia. In my country, it is widely distributed in Northeast China, North China, and Northwest China. Miscanthus is an annual dwarf herbaceous plant that likes warmth and is a wide-ranging xerophyte. It is an excellent forage grass and is favored by goats, sheep, horses, and camels in summer and autumn.

[0003] Existing studies have shown that the germination rate of Miscanthus seeds is low, at about 50 percent. At present, in order to improve the germination rate of Miscanthus seeds, different light intensities or friction to break the seed coat are used. Among them, the method of using different light intensities to improve the germination rate of Miscanthus seeds uses different light intensities to determine the optimal light intensity for Miscanthus seed germination. The method of using friction to break the seed coat to improve the germination rate of Miscanthus seeds is to break the seed coat of Miscanthus seeds by friction to promote seed germination. Both of the above methods have the problem of cumbersome operation and are not easy to combine with actual planting conditions. Summary of the invention

[0004] In order to solve the problem that the methods for improving the germination rate of Miscanthus sinensis seeds in the prior art are complicated to operate and difficult to combine with actual planting conditions, the present invention aims to break the dormancy of Miscanthus sinensis seeds, thereby promoting the germination of Miscanthus sinensis seeds, significantly improving the germination rate of Miscanthus sinensis seeds, and further providing a method for promoting the germination of Miscanthus sinensis seeds. To achieve the above purpose, the present invention adopts the following technical scheme:

[0005] The present invention provides a method for promoting the germination of Miscanthus sinensis seeds, comprising the following steps:

[0006] Before the Miscanthus sinensis seeds germinate, the Miscanthus sinensis seeds are pretreated with a seed soaking agent; after the pretreatment, the Miscanthus sinensis seeds are placed on filter paper in a culture dish and placed in the sun for germination.

[0007] The seed soaking agent is a solution containing a plant growth regulator; the concentration of the plant growth regulator in the seed soaking agent is 5 mg / L to 160 mg / L.

[0008] Plant growth regulators are also commonly referred to as plant hormones, which refer to artificially synthesized chemical substances with hormonal activity. According to their effects on plant growth, they are divided into three categories: plant growth promoters, plant growth retardants, and plant growth inhibitors. The plant growth regulator in the present invention is a plant hormone plant growth promoter used to promote plant growth. Plant hormones are also called plant growth promoters.

[0009] The above-mentioned plant hormones have many functions, such as significantly promoting cell division and tissue proliferation, and playing an important regulatory role in seed germination, leaf extension, stem and root elongation, flower and fruit development, etc. They are widely used in the daily management of crops, and gibberellins and brassinolide have more significant functions in promoting seed germination. Therefore, the present invention selects gibberellins and brassinolide as two plant hormones as seed soaking agents.

[0010] That is, the plant growth regulator described in the present invention is gibberellin or epibrassinolide.

[0011] The promoting of Miscanthus sinensis seed germination refers to increasing the seed germination speed, germination rate, germination potential and germination index.

[0012] Preferably, the pretreatment method is: soaking the Miscanthus seeds in the seed soaking agent for 24 to 72 hours. The mass ratio of the Miscanthus seeds to the volume of the seed soaking agent is 700 g / cm 3 ~900g / cm 3 , the soaking temperature is 18℃~25℃. This soaking material-liquid ratio can fully soak the seeds and maximize the effect of the seed soaking agent.

[0013] Combined with the conclusions of most papers related to seed germination, the most suitable soaking time for grass seeds germination is 24 to 72 hours.

[0014] Preferably, before soaking the Miscanthus seeds in the seed soaking agent, the Miscanthus seeds are screened. The purpose of the screening is to select Miscanthus seeds with uniform size and full grains. The Miscanthus seeds with uniform size and full grains are selected to reduce the experimental error and control the basic germination ability of the seeds to be consistent.

[0015] Preferably, the temperature during seed germination is 20°C to 27°C, the light intensity is 1500lx to 4000lx, and the humidity is 40% to 60%. Since the temperature and light intensity are not controlled in the experiment, the temperature and light intensity during seed germination are the natural temperature and light intensity during the experiment.

[0016] Preferably, the plant growth regulator is any one of gibberellins and epibrassinolide. The above two plant growth regulators both have the function of promoting seed germination. The present invention aims to explore which growth regulator has a very significant effect on promoting the germination of Miscanthus seeds.

[0017] Preferably, the concentration of gibberellin in the seed soaking agent is 5 mg / L to 160 mg / L; the concentration of epibrassinolide in the seed soaking agent is 5 mg / L to 20 mg / L.

[0018] Preferably, the concentration of gibberellin in the seed soaking agent is 10 mg / L, 20 mg / L, 30 mg / L, 50 mg / L, 100 mg / L or 150 mg / L. The concentration of epibrassinolide in the seed soaking agent is 5 mg / L, 10 mg / L or 15 mg / L.

[0019] Since no one has explored the germination of Miscanthus seeds, the optimal concentration range is proposed to be set by referring to the conclusions of seed germination experiments of other Poaceae forage grasses.

[0020] Wherein, when the plant growth regulator is gibberellin, the concentration of gibberellin in the seed soaking agent is 10mg / L to 150mg / L. Since no one has explored the field of Miscanthus guanyin seed germination, the present invention refers to the conclusions of other Gramineae forage seed germination tests to set the optimal concentration range.

[0021] When the plant growth regulator is brassinolide, the concentration of brassinolide in the seed soaking agent is 5 mg / L to 15 mg / L. Since no one has explored the field of Miscanthus guanyin seeds germination, the present invention refers to the conclusions of other Gramineae forage seeds germination tests to set the optimal concentration range.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention provides a method for promoting the germination of Miscanthus sinensis seeds. The method for promoting the germination of Miscanthus sinensis seeds provided by the present invention can effectively improve the germination rate of Miscanthus sinensis seeds and promote seed germination. The method for promoting the germination of Miscanthus sinensis seeds provided by the present invention only requires soaking the seeds in a prepared seed soaking agent, which has the advantages of simple operation and easier to combine with actual planting, and solves technical problems such as cumbersome operation and difficulty in combining with actual application.

[0024] 2. The method for promoting the germination of Miscanthus seeds provided by the present invention can effectively improve the germination rate of Miscanthus seeds, among which brassinolide can greatly increase the germination rate of Miscanthus seeds, from about 50% to 95%, thereby enriching the diversity of forage species and providing theoretical and technical support for promoting the germination of Miscanthus seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The figure is a test result diagram of the germination rate of Miscanthus sinensis seeds under different seed soaking agents, different seed soaking dosages and different seed soaking times in the present invention.

[0026] Figure 2 The figure is a test result diagram of the germination potential of Miscanthus sinensis seeds under different seed soaking agents, different seed soaking dosages and different seed soaking times in the present invention. DETAILED DESCRIPTION

[0027] The following will be combined with the specific implementation of the present invention to clearly and completely describe the technical solution in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The methods described in the embodiments of the present invention are conventional methods unless otherwise specified. The materials, reagents, etc. used can be obtained from commercial sources unless otherwise specified.

[0029] In the following examples, the reagents and materials are as follows:

[0030] The seeds of Miscanthus guanyin were obtained from the Agricultural and Animal Husbandry Science and Technology Demonstration Park of Inner Mongolia University for Nationalities.

[0031] Gibberellic acid was sourced from Beijing Solebow Technology Co., Ltd.

[0032] Epibrassinolide was obtained from Beijing Solebow Technology Co., Ltd.

[0033] Example 1

[0034] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0035] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0036] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0037] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 10mg / L.

[0038] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0039] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0040] Example 2

[0041] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0042] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0043] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0044] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 20mg / L.

[0045] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0046] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0047] Example 3

[0048] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0049] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0050] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0051] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 30mg / L.

[0052] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0053] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0054] Example 4

[0055] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0056] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0057] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0058] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 50mg / L.

[0059] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0060] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0061] Example 5

[0062] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0063] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0064] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray 5 mL of water on the filter paper until it is moistened.

[0065] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 100mg / L.

[0066] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0067] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0068] Example 6

[0069] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0070] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0071] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0072] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 24h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 150mg / L.

[0073] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0074] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0075] Example 7

[0076] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0077] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0078] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0079] S3, 0.015 g of Miscanthus sinensis seeds were soaked in 1 L of seed soaking agent, the soaking time was set to 24 h, and the soaking temperature was 20° C. The seed soaking agent was a solution containing brassinolide, and the concentration of epibrassinolide in the seed soaking agent was 5 mg / L.

[0080] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0081] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0082] Example 8

[0083] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0084] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0085] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0086] S3, soak 0.015g of Miscanthus sinensis seeds in 1L of seed soaking agent, the soaking time is set to 24h, and the soaking temperature is 20°C. The seed soaking agent is a solution containing brassinolide; the concentration of epibrassinolide in the seed soaking agent is 10mg / L.

[0087] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0088] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0089] Example 9

[0090] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0091] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0092] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0093] S3, soak 0.015g of Miscanthus sinensis seeds in 1L of seed soaking agent, the soaking time is set to 24h, and the soaking temperature is 20°C. The seed soaking agent is a solution containing brassinolide; the concentration of epibrassinolide in the seed soaking agent is 15mg / L.

[0094] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0095] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0096] Example 10

[0097] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0098] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0099] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0100] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 10mg / L.

[0101] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0102] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0103] Embodiment 11

[0104] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0105] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0106] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0107] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 20mg / L.

[0108] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0109] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0110] Example 12

[0111] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0112] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0113] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0114] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 30mg / L.

[0115] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0116] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0117] Embodiment 13

[0118] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0119] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm3 .

[0120] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0121] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 50mg / L.

[0122] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0123] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0124] Embodiment 14

[0125] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0126] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0127] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0128] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 100mg / L.

[0129] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0130] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0131] Embodiment 15

[0132] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0133] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0134] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0135] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 48h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 150mg / L.

[0136] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0137] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0138] Example 16

[0139] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0140] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0141] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0142] S3, soak 0.015g of Miscanthus sinensis seeds in 1L of seed soaking agent, the soaking time is set to 48h, and the soaking temperature is 20°C. The seed soaking agent is a solution containing brassinolide; the concentration of epibrassinolide in the seed soaking agent is 5mg / L.

[0143] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0144] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0145] Embodiment 17

[0146] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0147] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0148] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0149] S3, 0.015 g of Miscanthus sinensis seeds were soaked in 1 L of seed soaking agent, the soaking time was set to 48 h, and the soaking temperature was 20° C. The seed soaking agent was a solution containing brassinolide, and the concentration of epibrassinolide in the seed soaking agent was 10 mg / L.

[0150] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0151] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0152] Embodiment 18

[0153] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0154] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0155] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0156] S3, soak 0.015g of Miscanthus sinensis seeds in 1L of seed soaking agent, the soaking time is set to 48h, and the soaking temperature is 20°C. The seed soaking agent is a solution containing brassinolide; the concentration of epibrassinolide in the seed soaking agent is 15mg / L.

[0157] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0158] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0159] Embodiment 19

[0160] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0161] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0162] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0163] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 10mg / L.

[0164] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0165] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0166] Embodiment 20

[0167] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0168] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0169] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0170] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 20mg / L.

[0171] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0172] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0173] Embodiment 21

[0174] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0175] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0176] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0177] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 30mg / L.

[0178] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0179] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0180] Embodiment 22

[0181] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0182] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0183] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0184] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 50mg / L.

[0185] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0186] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0187] Embodiment 23

[0188] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0189] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0190] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0191] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 100mg / L.

[0192] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0193] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0194] Embodiment 24

[0195] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0196] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0197] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0198] S3, 0.015g of Miscanthus sinensis seeds were soaked in 1L of seed soaking agent, the soaking time was set to 72h, and the soaking temperature was 20°C. The seed soaking agent was a solution containing gibberellin, and the concentration of gibberellin in the seed soaking agent was 150mg / L.

[0199] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0200] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0201] Embodiment 25

[0202] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0203] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0204] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0205] S3, 0.015 g of Miscanthus sinensis seeds were soaked in 1 L of seed soaking agent, the soaking time was set to 72 h, and the soaking temperature was 20° C. The seed soaking agent was a solution containing brassinolide, and the concentration of epibrassinolide in the seed soaking agent was 5 mg / L.

[0206] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0207] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0208] Embodiment 26

[0209] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0210] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0211] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0212] S3, 0.015 g of Miscanthus sinensis seeds were soaked in 1 L of seed soaking agent, the soaking time was set to 72 h, and the soaking temperature was 20° C. The seed soaking agent was a solution containing brassinolide, and the concentration of epibrassinolide in the seed soaking agent was 10 mg / L.

[0213] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0214] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0215] Embodiment 27

[0216] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0217] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0218] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0219] S3, 0.015 g of Miscanthus sinensis seeds were soaked in 1 L of seed soaking agent, the soaking time was set to 72 h, and the soaking temperature was 20° C. The seed soaking agent was a solution containing brassinolide, and the concentration of epibrassinolide in the seed soaking agent was 15 mg / L.

[0220] S4. Use filter paper to absorb the soaking agent on the surface of the soaked seeds, place 20 selected Miscanthus seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0221] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0222] Comparative Example 1

[0223] A method for promoting the germination of Miscanthus sinensis seeds comprises the following steps:

[0224] S1. Seed screening: Screening is to select the seeds of Miscanthus sinensis with uniform size and full grains. The diameter / volume size of the screened Miscanthus sinensis seeds is 0.5mm / 0.02mm 3 .

[0225] S2. Spread a double layer of filter paper with a thickness of 0.35 mm in a culture dish and spray an equal amount of water on the filter paper until it is moistened.

[0226] S3. No soaking treatment is performed.

[0227] S4. Place twenty selected Miscanthus sinensis seeds in a culture dish, and spray 5 mL of water on the seeds every day during the period of vigorous photosynthesis to promote seed germination.

[0228] The temperature for germination of Miscanthus sinensis seeds was 24°C, the light intensity was 2500lx, and the humidity was 50%.

[0229] The above-mentioned Examples 1 to 27 and Comparative Example 1 were all treated by three repetitions, and a total of 82 culture dishes including the control group were used, 20 seeds were placed in each culture dish, and a total of 1,640 Miscanthus seeds were placed.

[0230] In order to illustrate the effect of the method for promoting the germination of Miscanthus seeds provided by the present invention, we take the above-mentioned Examples 1 to 27 and Comparative Example 1 as examples, and observe and record the Miscanthus seeds treated therewith respectively. The specific operations are as follows:

[0231] Observe the germination of Miscanthus chinensis every day, and the experiment is considered to be over if there is no new germination within 4 days. The germination is successful when the bud breaks through the seed coat by 3mm; the germination is considered to be a failure if the bud is deformed, damaged, rotten or no bud appears; the experiment is considered to be over if there is no new germination for four consecutive days. The final number of germinations in each culture dish is counted as K1, and its calculation formula is as follows:

[0232] Seed germination rate = (K1 / K) × 100%;

[0233] Where: K1 is the final germination number of each culture dish, and K is the total number of seeds tested in each culture dish.

[0234] Seed germination potential = (K2 / K) × 100%;

[0235] Where: K2 is the number of seeds germinated on the fourth day in each culture dish, and K is the total number of seeds tested in each culture dish.

[0236] According to the above calculation formula, the germination rates of Miscanthus sinensis seeds on the tenth day of germination in Examples 1 to 27 and Comparative Example 1 were calculated respectively. The results are shown in Figure 1 .

[0237] Depend on Figure 1It can be seen that the germination rate of seeds after soaking in a seed soaking agent is significantly higher than that of seeds not soaked in a seed soaking agent. In particular, in Example 26, after soaking in a seed soaking agent containing 10 mg / L brassinolide for 72 hours, the germination rate of Miscanthus seeds is as high as 95%, while the germination rate of Miscanthus seeds that have not been soaked in a seed soaking agent in Comparative Example 1 is only 55%.

[0238] From the above results, it can be seen that the germination rate of Miscanthus seeds can be greatly improved after soaking in a seed soaking agent containing 10 mg / L epibrassinolide for 72 hours. That is, the method for promoting the germination of Miscanthus seeds provided in Example 26 can / has the effect of breaking the dormancy of Miscanthus seeds and efficiently promoting the germination of Miscanthus seeds.

[0239] At the same time, we calculated the germination potential (germination index) of the Miscanthus sinensis seeds on the tenth day of germination in Examples 1 to 27 and Comparative Example 1 according to the above calculation formula. The results are shown in Figure 2 .

[0240] Depend on Figure 2 It can be seen that the germination potential of seeds after soaking in the seed soaking agent is significantly higher than that of seeds not soaked in the seed soaking agent. In particular, after soaking in the seed soaking agent containing 10 mg / L brassinolide for 72 hours in Example 26, the germination potential of Miscanthus seeds is as high as 95%, while the germination potential of Miscanthus seeds that have not been soaked in the seed soaking agent in Comparative Example 1 is only 35%.

[0241] And, combined with Figure 1 The results show that after soaking the Miscanthus seeds in the seed soaking agent containing 10 mg / L epibrassinolide for 72 hours in Example 26, the seed dormancy can be broken more quickly, which not only greatly improves the germination rate of the Miscanthus seeds, but also greatly improves the germination potential of the Miscanthus seeds. That is, the method for promoting the germination of Miscanthus seeds provided in Example 26 can / has the effect of breaking the dormancy of Miscanthus seeds and efficiently promoting the germination of Miscanthus seeds.

[0242] From the above results, it can be seen that soaking in a seed soaking agent containing 10 mg / L brassinolide for 72 hours can not only significantly increase the germination rate of Miscanthus seeds, but also significantly increase the germination potential of Miscanthus seeds. Moreover, this treatment only requires soaking the seeds in the prepared seed soaking agent, which has the advantages of simple operation and easier integration into actual planting, solving technical problems such as complicated operation and difficulty in combining with actual application.

[0243] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0244] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention belong to the scope of the present invention and its equivalent technology, the present invention is also intended to include these modifications and variations.

Claims

1. A method for promoting the germination of Miscanthus sinensis seeds, characterized in that: Before the Miscanthus sinensis seeds germinate, the Miscanthus sinensis seeds are pretreated with a seed soaking agent, and after the pretreatment, the seeds are germinated; The seed soaking agent is a solution containing a plant growth regulator; the concentration of the plant growth regulator in the seed soaking agent is 5 mg / L to 160 mg / L; The plant growth regulator is gibberellin or epibrassinolide; The promoting of Miscanthus sinensis seed germination refers to increasing the seed germination speed, germination rate, germination potential and germination index.

2. The method for promoting the germination of Miscanthus sinensis seeds according to claim 1, characterized in that: The pretreatment method is: soaking the Miscanthus sinensis seeds with a seed soaking agent, wherein the mass ratio of the Miscanthus sinensis seeds to the volume ratio of the seed soaking agent is 700 g / cm 3 ~900g / cm 3 .

3. The method for promoting the germination of Miscanthus sinensis seeds according to claim 2, characterized in that: The soaking time is 24 hours to 72 hours, and the soaking temperature is 18°C ​​to 25°C.

4. The method for promoting germination of Miscanthus sinensis seeds according to claim 1, characterized in that: The temperature of the Miscanthus sinensis seeds during germination is 20° C. to 27° C., the light intensity is 1500 lx to 4000 lx, and the humidity is 40% to 60%.

5. The method for promoting germination of Miscanthus sinensis seeds according to claim 2, characterized in that: The concentration of epibrassinolide in the seed soaking agent is 5 mg / L to 20 mg / L.

6. The method for promoting the germination of Miscanthus sinensis seeds according to claim 2, characterized in that: When the plant growth regulator is gibberellin, the concentration of gibberellin in the seed soaking agent is 10 mg / L to 150 mg / L.

7. The method for promoting the germination of Miscanthus sinensis seeds according to claim 6, characterized in that: The concentration of gibberellin in the seed soaking agent is 10 mg / L, 20 mg / L, 30 mg / L, 50 mg / L, 100 mg / L or 150 mg / L.

8. The method for promoting the germination of Miscanthus sinensis seeds according to claim 5, characterized in that: When the plant growth regulator is brassinolide, the concentration of brassinolide in the seed soaking agent is 5 mg / L to 15 mg / L.

9. The method for promoting germination of Miscanthus sinensis seeds according to claim 8, characterized in that: The concentration of epibrassinolide in the seed soaking agent is 5 mg / L, 10 mg / L or 15 mg / L.

10. The method for promoting germination of Miscanthus sinensis seeds according to claim 1, characterized in that: The Miscanthus sinensis seeds are selected from Miscanthus sinensis seeds with uniform size and full grains.

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