Composition with moisturizing and anti-allergy effects and application thereof

Abstract

The invention relates to a composition with moisturizing and anti-allergy effects and application thereof. The composition comprises a tea flower extract, a herba houttuyniae extract and a herba elsholtziae extract. The natural plant composition is prepared by taking water as an extraction solvent, and the obtained composition is safe, reliable and definite in biological activity, can synergistically improve skin moisture, allergy and inflammation, and has the effect of promoting skin health. The composition can be applied to the fields of food, health food, functional food or cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of material extraction, and relates to a composition with moisturizing and anti-allergic effects and application thereof. Background Art

[0002] The skin is the largest organ in the human body and undertakes many important functions, including barrier protection, temperature regulation and immune defense. The skin's ability to retain moisture and resist allergies is directly related to its health and protection capabilities. However, various factors in daily life, such as environmental pollution, ultraviolet exposure, poor eating habits and mental stress, can damage the skin's barrier function, leading to dry skin, allergic reactions and other skin problems.

[0003] As people's pursuit of beauty and health continues to improve, many oral beauty products have appeared on the market, aiming to improve skin moisture through internal regulation. A Chinese patent with application number CN 105582527 B discloses a composition for improving skin moisture and a preparation method thereof. The composition comprises okra, boletus, roselle, collagen, hyaluronic acid, sodium isovitaminosis C, and vitamin E, and has a good effect of improving skin moisture. A Chinese patent with application number CN 117694549 B discloses a composition, preparation method, and application for moisturizing, removing spots, and whitening. The composition comprises elastin peptide, tremella, black fruit glandular rib aronia fruit powder, Guanshan cherry powder, and plant lactobacillus S58, and has the effect of moisturizing and improving skin elasticity.

[0004] Existing technologies have explored oral products to improve skin moisture, but many product formulas contain too many raw materials, which may cause interference between ingredients, increase the complexity of the formula and production costs, and increase the risk of side effects or allergic reactions. In addition, improving skin moisture is a complex process that requires a scientific and reasonable combination of ingredients and comprehensive conditioning from multiple angles to achieve the best results. Summary of the invention

[0005] In view of the deficiencies in the prior art, the object of the present invention is to provide a composition with moisturizing and anti-allergic effects and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a composition with moisturizing and anti-allergic effects, the composition comprising a tea tree flower extract, a houttuynia cordata extract and an elsholtzia extract.

[0008] The present invention provides a plant composition for synergistically improving skin moisture and anti-allergy and a preparation method thereof, scientifically selecting and compatibility of three natural plants, namely, tea tree flower, houttuynia cordata and elsholtzia, by inhibiting hyaluronidase activity, promoting the expression of aquaporin AQP3, reducing the secretion of inflammatory factors, reducing allergic reactions, and promoting skin repair, thereby achieving the purpose of comprehensively improving skin moisture and enhancing skin barrier, fundamentally improving the skin's moisture retention capacity, and promoting skin health resistance. In addition, the composition formula design is simple and efficient, which can not only effectively avoid mutual interference between components, but also maximize the efficacy of each component. The polysaccharide content of the tea tree flower extract is not less than 2.5%, the flavonoid content in the houttuynia cordata extract is not less than 10%, the flavonoid content in the elsholtzia extract is not less than 35%, and the rosmarinic acid content is not less than 1.2%. The three extracts can cooperate with each other, and there is a potential synergistic effect.

[0009] Preferably, the composition comprises 3-10 parts of tea tree flower extract, 1-2 parts of houttuynia cordata extract and 1-5 parts of elsholtzia ciliata extract by weight.

[0010] The mass fraction of the tea tree flower extract can be selected from 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc., the mass fraction of the houttuynia cordata extract can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., the mass fraction of the Elsholtzia ciliata extract can be selected from 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, 4.8 parts, 5 parts, etc., and other specific point values ​​within the above numerical range can be selected, which will not be repeated here one by one.

[0011] Preferably, the composition is prepared by a method comprising the following steps: physically mixing the preparation raw materials to obtain.

[0012] Preferably, the tea tree flower extract is prepared by a method comprising the following steps:

[0013] (1) mixing tea tree flowers with water and performing vacuum reflux extraction to obtain a tea tree flower extract;

[0014] (2) The tea tree flower extract is filtered through a ceramic membrane and freeze-dried to obtain the tea tree flower extract.

[0015] Preferably, the Houttuynia cordata extract is prepared by a method comprising the following steps:

[0016] (1) mixing Houttuynia cordata with water, extracting, and obtaining a Houttuynia cordata extract;

[0017] (2) Concentrating the extract of Houttuynia cordata to obtain a concentrated solution, refrigerating the concentrated solution for flocculation, and centrifuging to obtain the product.

[0018] Preferably, the Elsholtzia ciliata extract is prepared by a method comprising the following steps:

[0019] (1) mixing Elsholtzia ciliata with water, then mixing with acid, and performing ultrasonic extraction to obtain an Elsholtzia ciliata extract;

[0020] (2) filtering the Elsholtzia ciliata extract through a ceramic membrane and drying the extract to obtain the product.

[0021] Preferably, the pore size of the ceramic membrane used in tea flower extraction is 200-400nm, for example 200nm, 220nm, 240nm, 260nm, 280nm, 300nm, 320nm, 340nm, 360nm, 380nm, 400nm, etc. Other specific point values ​​within the above numerical range can be selected, which will not be described here one by one.

[0022] Preferably, the pore size of the ceramic membrane used in the extraction of Elsholtzia ciliata is 50-100 nm, for example, 50 nm, 55 nm, 60 nm, 65 nm, 70 nm, 75 nm, 80 nm, 85 nm, 90 nm, 95 nm, 100 nm, etc. Other specific point values ​​within the above numerical range can be selected, which will not be described one by one here.

[0023] Preferably, the mass ratio of tea tree flowers to water is 1:(20-30).

[0024] Preferably, the mass ratio of Houttuynia cordata to water is 1:(10-15).

[0025] Preferably, the mass ratio of Elsholtzia ciliata to water is 1:(10-30).

[0026] Among them, the specific point values ​​in (20-30) can be selected from 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, etc., the specific point values ​​in (10-15) can be selected from 10, 11, 12, 13, 14, 15, etc., the specific point values ​​in (10-30) can be selected from 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be listed here one by one.

[0027] Preferably, the temperature of the reduced pressure reflux extraction is 60-70°C, the time is 1-2h, and the pressure is 0.02-0.08MPa.

[0028] The temperature can be selected as 60℃, 62℃, 64℃, 66℃, 68℃, 70℃, etc., the time can be selected as 1h, 1.1h, 1.2h, 1.3h, 1.4h, 1.5h, 1.6h, 1.7h, 1.8h, 1.9h, 2h, etc., and the pressure can be selected as 0.02MPa, 0.025MPa, 0.03MPa, 0.035MPa, 0.04MPa, 0.045MPa, 0.05MPa, 0.055MPa, 0.06MPa, 0.065MPa, 0.07MPa, 0.075MPa, 0.08MPa, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be elaborated here.

[0029] Preferably, the extraction temperature of Houttuynia cordata is 70-90°C and the extraction time is 40-60 min.

[0030] The temperature can be selected from 70°C, 72°C, 74°C, 76°C, 78°C, 80°C, 82°C, 84°C, 86°C, 88°C, 90°C, etc., and the time can be selected from 40min, 42min, 44min, 46min, 48min, 50min, 52min, 54min, 56min, 58min, 60min, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be elaborated here.

[0031] Preferably, before the extraction, the mixed solution of Houttuynia cordata and water is treated at 0.2-0.4 MPa and 120-140° C. for 5-10 min.

[0032] The pressure can be selected as 0.2MPa, 0.22MPa, 0.24MPa, 0.26MPa, 0.28MPa, 0.3MPa, 0.32MPa, 0.34MPa, 0.36MPa, 0.38MPa, 0.4MPa, etc. The temperature can be selected as 120℃, 122℃, 124℃, 126℃, 128℃, 130℃, 132℃, 134℃, 136℃, 138℃, 140℃, etc. The time can be selected as 5min, 6min, 7min, 8min, 9min, 10min, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be elaborated here.

[0033] Preferably, the acid comprises malic acid and / or citric acid.

[0034] Preferably, the mixing time with the acid is 5-20 min, for example 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 11 min, 12 min, 13 min, 14 min, 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, etc. Other specific points within the above numerical range can be selected, which will not be described here one by one.

[0035] Preferably, the solid content of the concentrated liquid is 15-25%, the refrigeration temperature is 2-4° C., and the time is 4-12 hours.

[0036] The solid content can be selected from 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25% and the like; the temperature can be selected from 2°C, 2.2°C, 2.4°C, 2.6°C, 2.8°C, 3°C, 3.2°C, 3.4°C, 3.6°C, 3.8°C, 4°C and the like; the time can be selected from 4h, 4.5h, 5h, 5.5h, 6h, 6.5h, 7h, 7.5h, 8h, 8.5h, 9h, 9.5h, 10h, 10.5h, 11h, 11.5h, 12h and the like; other specific point values ​​within the above numerical range can be selected, so they will not be described one by one here.

[0037] Preferably, the centrifugal speed is 6000-10000 rpm and the time is 5-10 min.

[0038] The rotation speed can be selected as 6000rpm, 6500rpm, 7000rpm, 7500rpm, 8000rpm, 8500rpm, 9000rpm, 9500rpm, 10000rpm, etc., and the time can be selected as 5min, 6min, 7min, 8min, 9min, 10min, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be described here one by one.

[0039] Preferably, the power of ultrasonic extraction is 200-500 W, the frequency is 20-50 kHz, the temperature is 65-80° C., the time is 40-60 min, and the pH is 3-4.5.

[0040] The pH can be selected from 3, 3.2, 3.4, 3.6, 3.8, 4, 4.1, 4.2, 4.3, 4.4, 4.5, etc. The power can be selected from 200W, 220W, 240W, 260W, 280W, 300W, 320W, 340W, 360W, 380W, 400W, 420W, 440W, 460W, 480W, 500W, etc. The frequency can be selected from 20kHz, 22kHz, 24kHz, 26kHz, 28kHz, 30kHz, 32kHz, 34kHz, 36kHz, 38kHz, 40kHz Hz, 42kHz, 44kHz, 46kHz, 48kHz, 50kHz, etc. The temperature can be selected as 65℃, 66℃, 67℃, 68℃, 69℃, 70℃, 71℃, 72℃, 73℃, 74℃, 75℃, 76℃, 77℃, 78℃, 79℃, 80℃, etc. The time can be selected as 40min, 42min, 44min, 46min, 48min, 50min, 52min, 54min, 56min, 58min, 60min, etc. Other specific point values ​​within the above numerical range can be selected, so I will not go into details here.

[0041] In a second aspect, the present invention provides a use of the composition with moisturizing and anti-allergic effects according to the first aspect in the preparation of a product with moisturizing, anti-allergic and anti-inflammatory effects.

[0042] Preferably, the product comprises food, cosmetics or health products.

[0043] Preferably, the added amount of the composition with moisturizing and anti-allergic effects in the product is 0.1-50%, for example 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, etc. Other specific point values ​​within the above numerical range can be selected, which will not be repeated here.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] (1) The present invention uses water as the extraction solvent, which can not only efficiently obtain the effective ingredients in the three natural herbal plants, but also does not use organic reagents throughout the process, is safer and more reliable, and has a wide range of applications.

[0046] (2) The composition prepared by the present invention can significantly inhibit hyaluronidase activity, promote the expression of AQP-3, inhibit NO secretion and the expression of TNF-α and tryptase, reduce the number of neutrophils in the zebrafish inflammation model, promote HaCat cell repair, improve skin moisture, anti-allergy, anti-inflammatory and repair effects, and promote skin health and water retention capacity in multiple ways.

[0047] (3) The composition prepared by the present invention has synergistic effects in improving skin moisture, anti-allergy, anti-inflammatory and repair effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 This is the result of zebrafish trypsin expression. ### indicates that compared with the control group, P<0.001; * indicates that compared with the model group, P<0.05; *** indicates that compared with the model group, P<0.001.

[0049] Figure 2 This is a fluorescent staining image of zebrafish neutrophils.

[0050] Figure 3 This is the result of zebrafish neutrophil count. ### indicates P<0.001 compared with the control group; *** indicates P<0.001 compared with the model group.

[0051] Figure 4 This is the microscope field result of the HaCat cell scratch repair test.

[0052] Figure 5 This is the healing rate result of HaCat cell scratch repair test. ** indicates P < 0.01 compared with the control group; *** indicates P < 0.001 compared with the control group. DETAILED DESCRIPTION

[0053] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only used to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0054] Preparation Example 1-1

[0055] This preparation example provides a method for preparing a tea tree flower extract, the preparation method comprising:

[0056] (1) The tea tree flowers were crushed and mixed with water, the mass ratio of tea tree flowers to water was 1:25, and refluxed for 1.5 h at 65° C. and 0.05 MPa;

[0057] (2) filtering the extract through a 600-mesh sieve and a 300-nm ceramic membrane in sequence;

[0058] (3) Sterilize the filtrate and freeze-dry it to obtain the product.

[0059] Preparation Example 1-2

[0060] This preparation example provides a method for preparing a tea tree flower extract, the preparation method comprising:

[0061] (1) The tea tree flowers were crushed and mixed with water, with the mass ratio of tea tree flowers to water being 1:20, and refluxed for extraction at 60°C and 0.02 MPa for 2 h;

[0062] (2) filtering the extract through a 600-mesh sieve and a 200 nm ceramic membrane in sequence;

[0063] (3) The filtrate is centrifuged, sterilized, and freeze-dried to obtain the product.

[0064] Preparation Example 1-3

[0065] This preparation example provides a method for preparing a tea tree flower extract, the preparation method comprising:

[0066] (1) The tea tree flowers were crushed and mixed with water, with the mass ratio of tea tree flowers to water being 1:30, and refluxed for extraction at 70°C and 0.08 MPa for 1 h;

[0067] (2) filtering the extract through a 600-mesh sieve and a 400-nm ceramic membrane in sequence;

[0068] (3) The filtrate is centrifuged, sterilized, and freeze-dried to obtain the product.

[0069] Preparation Example 2-1

[0070] This preparation example provides a method for preparing a Houttuynia cordata extract, the preparation method comprising:

[0071] (1) crushing Houttuynia cordata and mixing it with water, the mass ratio of Houttuynia cordata to water being 1:12, treating it at 0.3 MPa and 130° C. for 8 min, and extracting it at 80° C. for 50 min to obtain an extract;

[0072] (2) The extract is concentrated to a solid content of 20%, the concentrate is refrigerated at 3°C ​​for 8 hours, centrifuged at 8000 rpm for 8 minutes, the supernatant is sterilized, and dried to obtain the product.

[0073] Preparation Example 2-2

[0074] This preparation example provides a method for preparing a Houttuynia cordata extract, the preparation method comprising:

[0075] (1) crushing Houttuynia cordata and mixing it with water, the mass ratio of Houttuynia cordata to water being 1:15, treating it at 0.2 MPa and 120° C. for 10 min, and extracting it at 70° C. for 60 min to obtain an extract;

[0076] (2) The extract was concentrated to a solid content of 15%, the concentrate was refrigerated at 2°C for 4 hours, centrifuged at 6000 rpm for 10 minutes, the supernatant was sterilized and dried to obtain the product.

[0077] Preparation Example 2-3

[0078] This preparation example provides a method for preparing a Houttuynia cordata extract, the preparation method comprising:

[0079] (1) crushing Houttuynia cordata and mixing it with water, the mass ratio of Houttuynia cordata to water being 1:10, treating it at 0.4 MPa and 140° C. for 5 min, and extracting it at 90° C. for 40 min to obtain an extract;

[0080] (2) The extract was concentrated to a solid content of 25%, the concentrate was refrigerated at 4°C for 12 hours, centrifuged at 10,000 rpm for 5 minutes, the supernatant was sterilized, and dried to obtain the product.

[0081] Preparation Example 2-4

[0082] This preparation example provides a preparation method of Houttuynia cordata extract, which differs from Preparation Example 2-1 only in that step (1) is "crushing Houttuynia cordata and mixing with water, the mass ratio of Houttuynia cordata to water being 1:12, extracting at 80°C for 50 minutes to obtain an extract", and other operations remain unchanged.

[0083] Preparation Example 2-5

[0084] This preparation example provides a preparation method of Houttuynia cordata extract, which differs from Preparation Example 2-1 only in that step (2) is "concentrating the extract to a solid content of 10%, refrigerating the concentrate at 3°C ​​for 8 hours, centrifuging at 8000 rpm for 8 minutes, sterilizing the supernatant, and drying to obtain", and other operations remain unchanged.

[0085] Preparation Example 2-6

[0086] This preparation example provides a preparation method of Houttuynia cordata extract, which differs from Preparation Example 2-1 only in that step (2) is "concentrating the extract to a solid content of 30%, refrigerating the concentrate at 3°C ​​for 8 hours, centrifuging at 8000 rpm for 8 minutes, sterilizing the supernatant, and drying to obtain", and other operations remain unchanged.

[0087] Preparation Example 2-7

[0088] This preparation example provides a method for preparing Houttuynia cordata extract, which differs from Preparation Example 2-1 only in that step (2) is "concentrating the extract to a solid content of 20%, centrifuging at 8000 rpm for 8 minutes, sterilizing the supernatant, and drying to obtain", and other operations remain unchanged.

[0089] Preparation Example 2-8

[0090] This preparation example provides a preparation method of Houttuynia cordata extract, which differs from Preparation Example 2-1 only in that step (2) is "concentrating the extract to a solid content of 20%, refrigerating the concentrate at 3°C ​​for 8 hours, passing the refrigerated concentrate through a 400-mesh sieve, sterilizing the supernatant, and drying to obtain", and other operations remain unchanged.

[0091] Preparation Example 3-1

[0092] This preparation example provides a preparation method of Elsholtzia ciliata extract, the preparation method comprising:

[0093] (1) crushing Elsholtzia ciliata, mixing it with water, the mass ratio of Elsholtzia ciliata to water being 1:20, and then mixing it with citric acid for 5 minutes, adjusting the pH to 4, and obtaining a mixed solution;

[0094] (2) subjecting the extract to ultrasonic extraction at a power of 300 W, a frequency of 35 kHz, a temperature of 70° C., and a time of 50 min, filtering through an 80 nm ceramic membrane, concentrating the filtrate, sterilizing, and drying to obtain the product.

[0095] Preparation Example 3-2

[0096] This preparation example provides a preparation method of Elsholtzia ciliata extract, the preparation method comprising:

[0097] (1) crushing Elsholtzia ciliata, mixing it with water, the mass ratio of Elsholtzia ciliata to water being 1:10, and then mixing it with citric acid for 10 minutes, adjusting the pH to 3.5, and obtaining a mixed solution;

[0098] (2) subjecting the extract to ultrasonic extraction at a power of 200 W, a frequency of 20 kHz, a temperature of 65° C., and a time of 60 min; filtering the extract through a 100 nm ceramic membrane; concentrating the filtrate, sterilizing it, and drying it to obtain the product.

[0099] Preparation Example 3-3

[0100] This preparation example provides a preparation method of Elsholtzia ciliata extract, the preparation method comprising:

[0101] (1) crushing Elsholtzia ciliata, mixing it with water, the mass ratio of Elsholtzia ciliata to water being 1:30, and then mixing it with citric acid for 15 minutes, adjusting the pH to 4.5, and obtaining a mixed solution;

[0102] (2) subjecting the extract to ultrasonic extraction at a power of 500 W, a frequency of 50 kHz, a temperature of 80° C., and a time of 40 min, filtering through a 50 nm ceramic membrane, concentrating the filtrate, sterilizing, and drying to obtain the product.

[0103] Preparation Example 3-4

[0104] This preparation example provides a preparation method of an Elsholtzia ciliata extract, which differs from Preparation Example 3-1 only in that step (1) is "crushing the Elsholtzia ciliata and mixing it with water, wherein the mass ratio of the Elsholtzia ciliata to water is 1:20 to obtain a mixed solution", and other operations remain unchanged.

[0105] Preparation Example 3-5

[0106] This preparation example provides a preparation method of Elsholtzia ciliata extract, which differs from Preparation Example 3-1 only in that step (2) is "extracting the mixed solution at 70°C for 50 minutes, filtering through an 80nm ceramic membrane, concentrating the filtrate, sterilizing, and drying to obtain", and other operations remain unchanged.

[0107] Preparation Example 3-6

[0108] This preparation example provides a preparation method of Elsholtzia ciliata extract, which differs from Preparation Example 3-1 only in that step (2) is "heating the extract under reflux for 50 min, filtering through an 80 nm ceramic membrane, concentrating the filtrate, sterilizing, and drying to obtain the extract.

[0109] Example 1

[0110] This embodiment provides a composition with moisturizing and anti-allergic effects, which comprises, by mass, 5 parts of the tea tree flower extract described in Preparation Example 1-1, 1.5 parts of the Houttuynia cordata extract described in Preparation Example 2-1, and 3 parts of the Elsholtzia ciliata extract described in Preparation Example 3-1.

[0111] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0112] Example 2

[0113] This embodiment provides a composition with moisturizing and anti-allergic effects, which comprises, by mass, 10 parts of the tea tree flower extract described in Preparation Example 1-2, 1 part of the Houttuynia cordata extract described in Preparation Example 2-2, and 1 part of the Elsholtzia ciliata extract described in Preparation Example 3-2.

[0114] The preparation method is similar to that of Example 1.

[0115] Example 3

[0116] This embodiment provides a composition with moisturizing and anti-allergic effects, which comprises, by mass, 3 parts of the tea tree flower extract described in Preparation Example 1-3, 2 parts of the Houttuynia cordata extract described in Preparation Example 2-3, and 5 parts of the Elsholtzia ciliata extract described in Preparation Example 3-3.

[0117] The preparation method is similar to that of Example 1.

[0118] Embodiment 4-8

[0119] This embodiment provides 5 compositions with moisturizing and anti-allergic effects, which are different from Example 1 only in that the Houttuynia cordata extract obtained in Preparation Example 2-1 is replaced by the Houttuynia cordata extracts obtained in Preparation Example 2-4, Preparation Example 2-5, Preparation Example 2-6, Preparation Example 2-7, and Preparation Example 2-8, respectively, and the other ingredients and contents remain unchanged.

[0120] The preparation method is similar to that of Example 1.

[0121] Examples 9-11

[0122] This example provides three compositions with moisturizing and anti-allergic effects, which differ from Example 1 only in that the Elsholtzia ciliata extract obtained in Preparation Example 3-1 is replaced by the Elsholtzia ciliata extracts obtained in Preparation Example 3-4, Preparation Example 3-5, and Preparation Example 3-6, respectively, and the other ingredients and contents remain unchanged.

[0123] The preparation method is similar to that of Example 1.

[0124] Comparative Example 1

[0125] This comparative example provides a composition with moisturizing and anti-allergic effects, which is different from Example 1 only in that it does not contain the tea tree flower extract, and its reduced mass is proportionally distributed to the masses of the Houttuynia cordata extract and the Elsholtzia ciliata extract, while the rest remains unchanged.

[0126] The preparation method is similar to that of Example 1.

[0127] Comparative Example 2

[0128] This comparative example provides a composition with moisturizing and anti-allergic effects, which is different from Example 1 only in that it does not contain Houttuynia cordata extract, and its reduced mass is proportionally distributed to the masses of Camellia sinensis flower extract and Elsholtzia ciliata extract, while the rest remains unchanged.

[0129] The preparation method is similar to that of Example 1.

[0130] Comparative Example 3

[0131] This comparative example provides a composition with moisturizing and anti-allergic effects, which is different from Example 1 only in that it does not contain the Elsholtzia ciliata extract, and its reduced mass is proportionally distributed to the masses of the Camellia sinensis flower extract and the Houttuynia cordata extract, while the rest remains unchanged.

[0132] The preparation method is similar to that of Example 1.

[0133] Test Example 1

[0134] Hyaluronidase inhibition effect test

[0135] Use 0.1mM acetate buffer to prepare 0.25mg / mL hyaluronidase (HAS) solution and 1mg / mL sodium hyaluronate solution. Use water as solvent to prepare 1mg / mL tannic acid and sample solution. The operation is shown in Table 1:

[0136] Table 1

[0137]

[0138]

[0139] A—absorbance value of sample solution B—absorbance value of sample blank C—absorbance value of control solution D—absorbance value of control blank

[0140] Table 2

[0141]

[0142]

[0143] It can be seen from the data in Table 2 that the composition described in the present application has an excellent hyaluronidase inhibitory effect, and the preparation method of Houttuynia cordata extract and Elsholtzia ciliata extract will affect the efficacy of the composition. Camellia sinensis flower extract, Houttuynia cordata extract and Elsholtzia ciliata extract have a certain synergistic effect on the above-mentioned effects, and the lack of any one of them will affect the efficacy of the composition.

[0144] Test Example 2

[0145] Moisturizing efficacy test

[0146] Aquaporin-3 (AQP-3) plays an important role in maintaining skin moisture and skin function. The experiment used fluorescent quantitative PCR to determine the effect of samples on the expression of AQP-3. HaCaT cells were inoculated in a 24-well plate. A normal control group and a sample administration group (120 μg / mL) were set up in an incubator (37°C, 5% CO 2 ) were incubated for 12 h. When the plating rate reached 40% to 60%, the cells were divided and dosed. After the administration, the cells were incubated in an incubator for 24 h. After the incubation, a total RNA extraction kit was used for RNA determination, and fluorescence quantitative PCR was used to detect AQP3 gene expression. The results are shown in Table 3.

[0147] Table 3

[0148]

[0149]

[0150] It can be seen from the data in Table 3 that the composition described in the present application can significantly promote the expression of AQP-3 and has excellent moisturizing effect, and the preparation method of Houttuynia cordata extract and Elsholtzia ciliata extract will affect the efficacy of the composition. Camellia sinensis flower extract, Houttuynia cordata extract and Elsholtzia ciliata extract have a certain synergistic effect on the above-mentioned effects, and the lack of any one of them will affect the efficacy of the composition.

[0151] Test Example 3

[0152] Anti-inflammatory efficacy assay

[0153] According to the results of CCK-8 assay, the test concentration of the sample was selected as 250 μg / mL to determine the anti-inflammatory efficacy of macrophage RAW264.7. The ELISA method was used to detect the inhibitory effect of the sample on TNF-α and NO. RAW264.7 cell suspension was prepared to 5×10 4 / mL, and inoculated it in a 24-well plate, 1000μL per well, and incubated for 24 hours. Set up a blank control group, a positive control group (5μM dexamethasone sodium acetate) and a sample group, add 250μg / mL of the sample to be tested to each well, and incubate at 37℃, 5% CO 2 After incubation in the incubator for 1 hour, lipopolysaccharide (LPS) with a final concentration of 1 μg / mL was added to each well except the blank control group, and the culture was continued for 24 hours. After the end, the cell supernatant was collected by low-temperature centrifugation, and the ELISA kit and nitric oxide detection kit were used to detect the content of TNF-α and NO according to the instructions. The results are shown in Table 4.

[0154] Table 4

[0155] Group Relative expression of TNF-α Relative expression of NO Example 1 0.63±0.01 0.37±0.02 Example 2 0.66±0.02 0.40±0.03 Example 3 0.65±0.01 0.38±0.01 Example 4 0.73±0.04 0.47±0.06 Example 5 0.72±0.04 0.46±0.01 Example 6 0.83±0.02 0.52±0.03 Example 7 0.85±0.03 0.57±0.02 Example 8 0.82±0.02 0.63±0.07 Example 9 0.78±0.02 0.76±0.03 Example 10 0.80±0.06 0.69±0.01 Embodiment 11 0.70±0.03 0.49±0.03 Comparative Example 1 0.79±0.03 0.59±0.01 Comparative Example 2 0.86±0.04 0.52±0.01 Comparative Example 3 0.96±0.05 0.71±0.02 Preparation Example 1-1 0.95±0.11 0.53±0.02 Preparation Example 2-1 0.88±0.06 0.82±0.01 Preparation Example 3-1 0.75±0.04 0.58±0.02 Positive Control 0.56±0.01 0.85±0.04 Model Group 0.96±0.08 1.01±0.03 Normal control group 0.02±0.00 0.13±0.03

[0156] It can be seen from the data in Table 4 that the composition described in the present application can significantly inhibit the production of NO and the expression of TNF-α, and has excellent anti-inflammatory effect. The preparation methods of Houttuynia cordata extract and Elsholtzia ciliata extract will affect the efficacy of the composition. Camellia sinensis flower extract, Houttuynia cordata extract and Elsholtzia ciliata extract have certain synergistic effects on the above-mentioned effects, and the lack of any one of them will affect the efficacy of the composition.

[0157] Test Example 4

[0158] Anti-allergic efficacy assay

[0159] Dinitrochlorobenzene induces a significant increase in serum tryptase levels. Tryptase complexes with the BAPNA substrate and has a maximum absorption peak at 405nm, which can be used to detect the degree of allergy.

[0160] Zebrafish rearing methods were carried out according to "The zebrafish book".

[0161] 2dpf wild-type AB strain zebrafish were randomly selected in a 12-well cell culture plate, and 10 zebrafish were treated in each well. Samples with a concentration of 100μg / mL were given in water (Example 1, Preparation Example 1-1, Preparation Example 2-1, Preparation Example 3-1), positive control sodium cromoglycate concentration of 1000μg / mL, and a normal control group and a model control group were set up at the same time, and 3 experiments were conducted in parallel. Except for the normal control group, the remaining experimental groups were given dinitrochlorobenzene at a concentration of 3μg / mL to establish a zebrafish allergy model. Each experimental group was given BAPNA at a concentration of 100μg / mL, and incubated in a biochemical incubator (28.5℃±1.0℃) in the dark for 22h, then the 12-well plate was taken out, and 200L of liquid was drawn from each well into a 96-well plate after shaking, and the relative expression level of tryptase was determined by reading the absorbance value at a wavelength of 405nm.

[0162]

[0163] Sample group: average absorbance of each sample group

[0164] Normal control group: the average value of the normal control absorbance

[0165] Sample blank group: absorbance value of the sample with the same volume

[0166] Normal blank group: absorbance value of embryo culture medium of the same volume

[0167] The results are as follows Figure 1 As shown by Figure 1 It can be seen that the composition described in the present application has an excellent anti-allergic effect and can significantly reduce the expression of trypsin, and the effect is better than that of tea tree flower extract, Elsholtzia extract and Houttuynia cordata extract.

[0168] Test Example 5

[0169] Anti-inflammatory efficacy assay

[0170] 3dpf transgenic neutrophil red fluorescent MPX were randomly selected in a 6-well plate, and 15 zebrafish were treated in each well (experimental group). The sample of Example 1 (test concentration was 100μg / mL) and the positive control dexamethasone acetate (concentration was 5μg / mL) were administered in water, and a normal control group and a model control group were set up at the same time, with a capacity of 3mL per well. Except for the normal control group, the remaining experimental groups were administered 1μM copper sulfate solution in water to establish a zebrafish skin inflammation model. After treatment at 28℃ for 2h, 10 zebrafish were randomly selected from each group and photographed under a fluorescence microscope and the pictures were saved ( Figure 2), and NIS-Elements D 3.20 advanced image processing software was used to analyze and collect data to analyze the number of neutrophils in zebrafish skin. Neutrophil red fluorescence labeled zebrafish (Tg(mpx:dsRED)) neutrophils in the body can emit red fluorescence, so the migration and other behaviors of neutrophils in living zebrafish can be directly observed under a stereo fluorescence microscope. After the incubation, zebrafish with normal phenotype and behavior were randomly selected, fixed with 3% methylcellulose, observed and photographed under a stereo fluorescence microscope, and the statistical results are shown as follows Figure 3 As shown by Figure 3 The results show that the composition described in the present application can significantly reduce the number of neutrophils and has an excellent anti-inflammatory effect.

[0171] Test Example 6

[0172] Scratch repair activity assay

[0173] HaCaT cells were seeded in 12-well plates and incubated at 37°C with 5% CO 2 The cells were cultured in an incubator with a maintenance medium for 24 h, and a plug-in cell scratch model was placed in each well of the culture plate and cultured in an incubator for 24 h. A normal control group (serum-free DMEM medium) and a positive control group (epidermal growth factor EGF solution concentration of 10000 IU / mL) and a test substance (Example 1, Preparation Example 1-1, Preparation Example 2-1, Preparation Example 3-1) treatment group with a concentration of 250 μg / mL were set up. The cell growth state at the scratch site was observed under a microscope for 24 hours, and photographed and recorded, respectively, as shown in FIG. Figure 4 As shown, the healing area was calculated using ImageJ software. The healing rate calculation formula is as follows:

[0174] Healing rate = (initial scratch area - scratch area at testing time) / initial scratch area.

[0175] The calculation results are as follows Figure 5 As shown by Figure 5 The results show that the composition described in the present application can significantly improve the cell healing rate, and the effect is better than that of tea tree flower extract, Elsholtzia extract and Houttuynia cordata extract.

[0176] The applicant declares that the present invention illustrates a composition with moisturizing and anti-allergic effects and its application through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0177] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0178] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition with moisturizing and anti-allergic effects, It is characterized in that The composition comprises tea tree flower extract, houttuynia cordata extract and elsholtzia ciliata extract.

2. The composition with moisturizing and anti-allergic effects according to claim 1, It is characterized in that The composition comprises 3-10 parts of tea tree flower extract, 1-2 parts of houttuynia cordata extract and 1-5 parts of elsholtzia ciliata extract by weight.

3. The composition with moisturizing and anti-allergic effects according to claim 1, It is characterized in that The tea tree flower extract is prepared by a method comprising the following steps: (1) mixing tea tree flowers with water and performing vacuum reflux extraction to obtain a tea tree flower extract; (2) The tea tree flower extract is filtered through a ceramic membrane and freeze-dried to obtain the tea tree flower extract.

4. The composition with moisturizing and anti-allergic effects according to claim 1, It is characterized in that The houttuynia cordata extract is prepared by a method comprising the following steps: (1) mixing Houttuynia cordata with water, extracting, and obtaining a Houttuynia cordata extract; (2) Concentrating the extract of Houttuynia cordata to obtain a concentrated solution, refrigerating the concentrated solution for flocculation, and centrifuging to obtain the product.

5. The composition with moisturizing and anti-allergic effects according to claim 1, It is characterized in that The Elsholtzia extract is prepared by a method comprising the following steps: (1) mixing Elsholtzia ciliata with water, then mixing with acid, and performing ultrasonic extraction to obtain an Elsholtzia ciliata extract; (2) filtering the Elsholtzia ciliata extract through a ceramic membrane and drying the extract to obtain the product.

6. The composition with moisturizing and anti-allergic effects according to claim 3, It is characterized in that The temperature of the reduced pressure reflux extraction is 60-70°C, the time is 1-2h, and the pressure is 0.02-0.08MPa.

7. The composition with moisturizing and anti-allergic effects according to claim 4, It is characterized in that Before the extraction, the mixed solution of Houttuynia cordata and water is treated at 0.2-0.4 MPa and 120-140° C. for 5-10 minutes.

8. The composition with moisturizing and anti-allergic effects according to claim 4, It is characterized in that The solid content of the concentrated liquid is 15-25%, and the refrigeration temperature is 2-4° C. for 4-12 hours.

9. The composition with moisturizing and anti-allergic effects according to claim 5, It is characterized in that The power of ultrasonic extraction is 200-500W, the frequency is 20-50kHz, the temperature is 65-80℃, the time is 40-60min, and the pH is 3-4.

5.

10. Use of the composition with moisturizing and anti-allergic effects according to any one of claims 1 to 9 in the preparation of products with moisturizing, anti-allergic or anti-inflammatory effects.

Images