Persistent deodorization type cat litter partner as well as preparation method and application thereof

Abstract

The invention relates to a persistent deodorization type cat litter partner as well as a preparation method and application thereof. The cat litter mate is prepared by compounding nano zinc chloride, bacillus subtilis powder and enterococcus faecium powder according to a specific proportion, and has an excellent deodorization effect. The bacillus subtilis and enterococcus faecium powder can secrete various enzymes and bacteriocin, effectively decompose odor substances and inhibit growth of putrefying bacteria, and tolerate the bactericidal effect of nano zinc chloride at the same time. The cat litter mate provided by the invention can significantly reduce the concentration of ammonia gas and volatile organic compounds, has the advantages of long-acting deodorization, environmental protection and safety, and is suitable for the field of pet breeding.

Description

Technical Field

[0001] The invention relates to a disinfecting and deodorizing cat litter companion for pets, a preparation method and application thereof, and belongs to the technical field of disinfection and deodorization. Background Art

[0002] With the acceleration of China's urbanization process, my country's pet industry has developed rapidly. For cat owners, cat litter issues and deodorization needs are very important factors. The choice of cat litter is very important for the health and hygiene of cats. A good cat litter should be able to quickly absorb urine, solidify cat litter, control the release of odor, and be easy to clean and replace.

[0003] At present, the cat litter deodorization technologies on the market mainly include adsorbent technology, fragrance technology, chemical reaction technology and biological technology. Adsorbent technology requires frequent replacement of cat litter, which undoubtedly increases the cost and energy of pet breeding; fragrance technology only uses fragrance to cover the odor, and cannot really remove the gas that produces the odor; chemical reaction technology often uses substances that have adverse effects on the health of cats and humans, and cannot completely eliminate odors.

[0004] Biological cat litter deodorizer uses a different approach. In biological cat litter deodorizer, the microorganisms are fixed in a porous material so that they can survive and reproduce for a long time. When cat urine enters the cat litter, the odorous substances in it are broken down by the microorganisms into harmless substances, thereby reducing or even eliminating the odor. In addition, biological cat litter deodorizer also has good hygroscopicity and antibacterial properties, ensuring the cleanliness and hygiene of the cat litter. It is also very easy to use. Just sprinkle it in the cat litter. Compared with traditional chemical deodorizers, it is safer, more environmentally friendly and can effectively remove odors. Summary of the invention

[0005] To solve the technical problems existing in the prior art, the present application provides a long-lasting deodorizing cat litter companion, which includes 4-8 parts of nano zinc chloride, 40-80 parts of Bacillus subtilis powder, and 20-40 parts of Enterococcus faecium powder, and the above percentages are mass ratios.

[0006] As a preferred technical solution for a long-lasting deodorizing cat litter companion, the particle size of the nano zinc oxide is 5-50 nanometers.

[0007] As an optimal technical solution for a long-lasting deodorizing cat litter companion, the mass ratio of nano zinc chloride to Bacillus subtilis powder and Enterococcus faecium powder is 5:65:30.

[0008] As a preferred technical solution for a long-lasting deodorizing cat litter companion, the preparation method of the Bacillus subtilis powder comprises the following technical steps:

[0009] Activation of strains: Streak Bacillus subtilis onto LB solid plates and culture at 37°C for 24 hours;

[0010] Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 200r / min, culture temperature 37℃, and culture time 24h;

[0011] Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 5% by volume, the loading coefficient is 60% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 4 L / min, a culture temperature of 37°C, and a culture time of 24 h, to obtain Bacillus subtilis fermentation solution;

[0012] Fermentation liquid culture medium: peptone 5g / L, beef extract 10g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.0.

[0013] Preparation of bacterial powder: 10% spray drying protective agent was added to the Bacillus subtilis fermentation liquid, and the Bacillus subtilis powder was obtained by low-temperature spray drying, with the number of live bacteria being 100 billion / g.

[0014] As a preferred technical solution for a long-lasting deodorizing cat litter companion, the preparation method of the Enterococcus faecium powder comprises the following technical steps:

[0015] Activation of bacterial strains: Streak Enterococcus faecalis onto LB solid plates and culture at 37°C for 48 hours;

[0016] Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 100r / min, culture temperature 37℃, and culture time 48h;

[0017] Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 10% by volume, the loading coefficient is 70% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 2 L / min, a culture temperature of 37°C, and a culture time of 48 h, to obtain the Enterococcus faecalis fermentation solution;

[0018] Fermentation liquid culture medium: peptone 5g / L, beef extract 5g / L, sodium acetate 5g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.0.

[0019] Preparation of bacterial powder: 10% spray drying protective agent was added to the fermentation liquid of Enterococcus faecium, and the Enterococcus faecium powder was obtained by low-temperature spray drying, with the number of live bacteria being 20 billion / g.

[0020] Another inventive aspect of the present invention is to use the cat litter companion as a deodorizing composition in a long-lasting deodorizing cat litter companion, and add the cat litter companion accounting for 1-5‰ of the cat litter into the cat litter and mix well for use.

[0021] Bacillus subtilis can secrete a variety of enzymes, such as proteases, lipases, amylases, etc., which can degrade substances from odor sources, such as ammonia, hydrogen sulfide, methyl mercaptan, etc., and convert them into odorless or low-odor substances through redox reactions. In addition, Bacillus subtilis and Enterococcus faecium secrete bacteriocins, which can effectively inhibit the growth of odor-producing spoilage bacteria, and can tolerate nano zinc chloride with bactericidal and disinfecting effects. The composite microecological bacterial agent prepared by effectively compounding Bacillus subtilis, Enterococcus faecium and nano zinc chloride in the present invention has a more excellent technical effect when used for deodorizing cat litter, and is expected to be widely used in pet breeding. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0023] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0025] Example 1

[0026] A method for preparing a cat litter companion comprises the following steps:

[0027] (1) Preparation of Bacillus subtilis powder

[0028] Activation of strains: Streak Bacillus subtilis onto LB solid plates and culture at 37°C for 24 hours;

[0029] Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 200r / min, culture temperature 37℃, and culture time 24h;

[0030] Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 5% by volume, the loading coefficient is 60% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 4 L / min, a culture temperature of 37°C, and a culture time of 24 h, to obtain Bacillus subtilis fermentation solution;

[0031] Fermentation liquid culture medium: peptone 5g / L, beef extract 10g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.0.

[0032] Preparation of bacterial powder: 10% spray drying protective agent was added to the Bacillus subtilis fermentation liquid, and the Bacillus subtilis powder was obtained by low-temperature spray drying, with the number of live bacteria being 100 billion / g.

[0033] (2) Preparation of Enterococcus faecium powder

[0034] Activation of strains: Streak Enterococcus faecalis onto LB solid plates and culture at 370°C for 48 hours;

[0035] Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 100r / min, culture temperature 37℃, and culture time 48h;

[0036] Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 10% by volume, the loading coefficient is 70% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 2 L / min, a culture temperature of 37°C, and a culture time of 48 h, to obtain the Enterococcus faecalis fermentation solution;

[0037] Fermentation liquid culture medium: peptone 5g / L, beef extract 5g / L, sodium acetate 5g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.0.

[0038] Preparation of bacterial powder: 10% spray drying protective agent was added to the fermentation liquid of Enterococcus faecium, and the Enterococcus faecium powder was obtained by low-temperature spray drying, with the number of live bacteria being 20 billion / g.

[0039] Preparation of compound cat litter companion: The prepared nano zinc chloride, Bacillus subtilis and Enterococcus faecium powder are mixed in a mass ratio of 5:65:30 to obtain a liquid cat litter companion, which is added into cat litter at a mass ratio of 2‰ for use.

[0040] The cat litter with long-lasting deodorization effect prepared in Example 1 was used as the experimental group, and the common cat litter on the market was used as the control group. The cat litter was spread into two clean cat litter boxes to form a layer of cat litter with a thickness of 7-10 cm, and the concentration of ammonia and VOC was detected.

[0041] Table 1 The degradation effect of cat litter companion in the experimental group on ammonia in cat litter

[0042]

[0043] Table 2 The degradation effect of cat litter companion in the experimental group on VOC in cat litter

[0044]

[0045] The results showed that the ammonia concentration in the experimental group of cat litter was reduced by 80% and VOC emissions were reduced by 70% compared with the control group.

[0046] Example 2

[0047] Bacillus subtilis powder is prepared according to the preparation process of Bacillus subtilis powder in Example 1, and Enterococcus faecalis powder is prepared according to the preparation process of Enterococcus faecalis powder in Example 1. The nano zinc oxide is preferably 5 nano zinc oxide.

[0048] Bacillus subtilis powder, Enterococcus faecalis powder and nano zinc oxide were separately mixed with cat litter (bentonite) for use, and the mixing ratio was powder (zinc oxide): cat litter = 2:1000. The cat litter mixed with Bacillus subtilis powder was recorded as experimental group K, the cat litter mixed with Enterococcus faecalis powder was recorded as experimental group F, the cat litter mixed with nano zinc oxide was recorded as experimental group X, and the blank cat litter was recorded as control group C. They were spread in clean cat litter boxes to form a layer of cat litter with a thickness of 7-10 cm, and the concentrations of ammonia and VOC were detected, and the results are recorded in Tables 3 and 4.

[0049] Table 3 Ammonia concentration (ppm) of ammonia degradation records in the experimental cat litter box

[0050]

[0051] Table 4 VOC degradation record in the experimental cat litter box VOC (ppm)

[0052]

[0053]

[0054] The test results show that the deodorizing effect of nano zinc oxide on cat litter is weaker than that of Bacillus subtilis powder and Enterococcus faecalis powder, which is in line with expectations. This is because nano zinc oxide mainly has an antibacterial effect and a weak adsorption effect, and Enterococcus faecalis has a significant effect on the degradation of ammonia.

[0055] Example 3

[0056] Bacillus subtilis powder is prepared according to the preparation process of Bacillus subtilis powder in Example 1, and Enterococcus faecalis powder is prepared according to the preparation process of Enterococcus faecalis powder in Example 1. The nano zinc oxide is preferably 25 nano zinc oxide.

[0057] Nano zinc oxide was mixed with Bacillus subtilis powder and Enterococcus faecalis powder in a ratio of nano zinc oxide: powder = 5:95, and then mixed with cat litter. The mixed Bacillus subtilis powder was recorded as experimental group KX, and the mixed Enterococcus faecalis powder was recorded as experimental group FX. Bacillus subtilis powder and Enterococcus faecalis powder were mixed in a ratio of 1:1 and recorded as experimental group FK. Blank cat litter was recorded as control group C. They were spread in clean cat litter boxes to form a layer of cat litter with a thickness of 7-10 cm. The concentrations of ammonia and VOC were detected and recorded as Table 5 and Table 6.

[0058] Table 5 Ammonia degradation records in experimental cat litter boxes

[0059]

[0060] Table 6 VOC degradation records in experimental cat litter boxes

[0061]

[0062] The test results show that when nano zinc oxide is mixed with bacterial powder alone, it has a certain enhancing effect on the bacterial powder. When the two bacterial powders are mixed, the deodorizing effect on cat litter is obvious.

[0063] In summary, when nano zinc chloride, Bacillus subtilis powder and Enterococcus faecium powder are used in combination, the deodorizing effect is significantly better than the effect of using any one of the substances alone or the combination of two substances. Specifically, the combined use of the three substances can more effectively reduce the concentration of ammonia and volatile organic compounds (VOCs), and significantly reduce the generation of odor. Compared with a single substance or a combination of two substances, the synergistic effect of the three substances not only enhances the decomposition efficiency of odorous substances, but also significantly inhibits the growth of spoilage bacteria, thereby achieving a better and lasting deodorizing effect. This composite use strategy embodies the principle of multi-component synergistic enhancement, proving that when the three substances are used in combination, their overall performance far exceeds the effect of using them alone.

[0064] Example 4

[0065] Bacillus subtilis powder is prepared according to the preparation process of Bacillus subtilis powder in Example 1, and Enterococcus faecalis powder is prepared according to the preparation process of Enterococcus faecalis powder in Example 1. The nano zinc oxide is preferably 5 nano zinc oxide.

[0066] Nano zinc oxide, Bacillus subtilis powder, and Enterococcus faecalis powder were uniformly mixed in the following proportions and recorded as experimental groups A, B, and C, and the unmixed cat litter was recorded as the blank control group K. They were spread in a clean cat litter box to form a layer of cat litter with a thickness of 7-10 cm, and the concentrations of ammonia and VOC were detected and recorded as Table 7 and Table 8.

[0067] Experimental group A: Nano zinc oxide: Bacillus subtilis powder: Enterococcus faecalis powder = 5:45:50

[0068] Experimental group B: nano zinc oxide: Bacillus subtilis powder: Enterococcus faecalis powder = 5:30:65

[0069] Experimental group C: Nano zinc oxide: Bacillus subtilis powder: Enterococcus faecalis powder = 5:65:30

[0070] Table 7 Ammonia degradation records in experimental cat litter box

[0071]

[0072] Table 8 VOC degradation records in experimental cat litter boxes

[0073]

[0074] The test results show that the deodorizing effect of nano zinc oxide mixed with Bacillus subtilis and Enterococcus faecalis powder is stronger than that of mixing the strains separately. The comprehensive results show that the deodorizing effect of nano zinc oxide: Bacillus subtilis powder: Enterococcus faecalis powder = 5:65:30 is the best. This may be because nano zinc chloride in this combination not only provides antibacterial and disinfection effects, but also produces a synergistic effect with the strains. Nano zinc chloride can selectively inhibit harmful bacteria without affecting the activity of beneficial bacteria, thereby making Bacillus subtilis and Enterococcus faecalis more advantageous in dealing with odors.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A long-lasting deodorizing cat litter companion, characterized in that: The cat litter companion comprises 4-8 parts of nano zinc chloride, 40-80 parts of Bacillus subtilis powder and 20-40 parts of Enterococcus faecium powder, and the above percentages are by mass ratio.

2. The persistent deodorizing cat litter companion according to claim 1, characterized in that: The particle size of the nano zinc oxide is 5-50 nanometers.

3. The persistent deodorizing cat litter companion according to claim 1, characterized in that, The mass ratio of nano zinc chloride to Bacillus subtilis powder and Enterococcus faecium powder is 5:65:

30.

4. The persistent deodorizing cat litter companion according to claim 1, characterized in that, The preparation method of the Bacillus subtilis powder comprises the following technical steps: Activation of strains: Streak Bacillus subtilis onto LB solid plates and culture at 37°C for 24 hours; Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 200r / min, culture temperature 37℃, and culture time 24h; Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 5% by volume, the loading coefficient is 60% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 4 L / min, a culture temperature of 37°C, and a culture time of 24 h, to obtain Bacillus subtilis fermentation solution; Fermentation liquid culture medium: peptone 5g / L, beef extract 10g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.

0. Preparation of bacterial powder: 10% spray drying protective agent was added to the Bacillus subtilis fermentation liquid, and the Bacillus subtilis powder was obtained by low-temperature spray drying, with the number of live bacteria being 100 billion / g.

5. The persistent deodorizing cat litter companion according to claim 1, characterized in that: The preparation method of the Enterococcus faecium powder comprises the following technical steps: Activation of bacterial strains: Streak Enterococcus faecalis onto LB solid plates and culture at 37°C for 48 hours; Seed culture: Pick a mature single colony and transfer it into LB liquid culture medium. The culture conditions are stirring speed 100r / min, culture temperature 37℃, and culture time 48h; Fermentation tank culture: the seed culture solution is added to the fermentation tank at an inoculation rate of 10% by volume, the loading coefficient is 70% by volume, the culture conditions are a stirring speed of 300 r / min, a ventilation volume of 2 L / min, a culture temperature of 37°C, and a culture time of 48 h, to obtain the Enterococcus faecalis fermentation solution; Fermentation liquid culture medium: peptone 5g / L, beef extract 5g / L, sodium acetate 5g / L, sodium chloride 5g / L, glucose 20g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 2g / L, pH 7.

0. Preparation of bacterial powder: 10% spray drying protective agent was added to the fermentation liquid of Enterococcus faecium, and the Enterococcus faecium powder was obtained by low-temperature spray drying, with the number of live bacteria being 20 billion / g.

6. The application of the cat litter companion described in any one of claims 1 to 5 as a deodorizing composition in a persistent deodorizing cat litter companion, characterized in that: Add 1-5‰ of cat litter companion to the cat litter and mix well before use.

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