Skin care product composition based on liposome technology as well as preparation method and application of skin care product composition

Abstract

The invention provides a skin care product composition based on a lipidosome technology and a preparation method and application thereof. The skin care product composition comprises the following components in percentage by weight: 0.5-5% of lipidosome; 1.2%-12% of protein peptide; 1.01%-4% of vitamin components; 0.3%-3% of hyaluronic acid; 1.51%-14% of an antioxidant component; 0.01%-1.5% of a cell protection component; and 0.51%-4.0% of lipid molecules. According to the skin care product composition based on the lipidosome technology and the preparation method and application of the skin care product composition, the multiple effects of moisturizing, repairing, whitening and aging resisting can be achieved, and various skin care requirements are met. Through a lipidosome wrapping technology, various active ingredients can be effectively wrapped, the stability and permeability are improved, and the skin care effect is enhanced. Natural active ingredients are adopted, so that irritation and side effects on skin are reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and more specifically, relates to a skin care product composition based on liposome technology, a preparation method and application thereof. Background Art

[0002] Currently, there are many skin care products on the market that can moisturize, repair, whiten, tighten and anti-wrinkle. However, most products have a single function or use chemical additives to achieve the effect, which may irritate the skin or have side effects after long-term use. In addition, most of the existing multifunctional skin care products fail to effectively combine various active ingredients, resulting in unsatisfactory results.

[0003] The active ingredients of traditional skin care products are simply compounded with single compounds. Firstly, it is difficult for them to penetrate the skin and take effect. In addition, excessive local concentration of active ingredients on the skin surface in a short period of time may cause skin toxicity.

[0004] In summary, existing products have the following problems: the product has a single function and cannot meet the multiple needs of moisturizing, repairing, whitening and anti-aging at the same time. The use of chemical additives may cause irritation or side effects to the skin. The various ingredients in multifunctional skin care products fail to work together effectively, resulting in an insignificant overall effect. Summary of the invention

[0005] The purpose of the present invention is to provide a prosthetic foot inspection machine, aiming to solve the above technical problems existing in the prior art.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: to provide a skin care composition based on liposome technology, characterized in that it includes the following components in weight percentage: 0.5%-5% liposomes; 1.2%-12% protein peptides; 1.01%-4% vitamin ingredients; 0.3%-3% hyaluronic acid; 1.51%-14% antioxidant ingredients; 0.01%-1.5% cell protection ingredients; 0.51%-4.0% lipid molecules.

[0007] Preferably, the protein peptides include: one or more of collagen peptides, elastin peptides, carnosine, and decarboxylated carnosine.

[0008] Preferably, the antioxidant ingredients include: one or more of ferulic acid, ergothioneine, boraxine, and α-arbutin.

[0009] Preferably, the vitamin components include: one or more of tocopherol, ascorbyl palmitate, and retinol palmitate.

[0010] Preferably, the lipid molecules include: one or more of ceramide, phosphatidylcholine, and cholesterol.

[0011] Preferably, the liposome is 4-butylresorcinol liposome.

[0012] Preferably, the antioxidant ingredients are ferulic acid, ergothioneine, bosine, and α-arbutin; wherein the content of ferulic acid is 0.5%-5%; the content of ergothioneine is 0.01%-2.5%; the content of bosine is 0.5%-5%; and the content of α-arbutin is 0.5%-1.5%.

[0013] Preferably, the vitamin components include tocopherol, ascorbyl palmitate, and retinol palmitate; the content of the tocopherol is 0.5%-2.0%; the content of the ascorbyl palmitate is 0.01%-0.5%; and the content of the retinol palmitate is 0.01%-0.5%.

[0014] A method for preparing the above-mentioned skin care composition based on liposome technology, characterized by comprising:

[0015] The raw materials are divided into water-soluble components and fat-soluble components; the water-soluble components are dissolved in water for later use; the fat-soluble components are dissolved in ethanol for later use;

[0016] The products were prepared by thin film hydration method.

[0017] An essence lotion, characterized in that it comprises a skin care composition based on liposome technology as described in any one of the above items or a skin care composition based on liposome technology prepared by any one of the preparation methods described in the above items.

[0018] The beneficial effect of the artificial foot inspection machine provided by the present invention is that compared with the prior art, the artificial foot inspection machine provided by the present invention can achieve multiple functions of moisturizing, repairing, whitening and anti-aging, and meet various skin care needs. Through the liposome encapsulation technology, various active ingredients can be effectively encapsulated, their stability and permeability can be improved, and the skin care effect can be enhanced. Natural active ingredients are used to reduce skin irritation and side effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 The effect of preparation conditions on the average particle size and encapsulation efficiency of the skin care composition. DETAILED DESCRIPTION

[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] See also Figure 1 Now, a skin care product composition based on liposome technology provided by the present invention is described. The skin care product composition based on liposome technology comprises: liposomes and active ingredients, wherein the liposomes encapsulate the active ingredients; wherein the active ingredients comprise: protein peptides, vitamin ingredients, hyaluronic acid, antioxidant ingredients, whitening ingredients, cell protection ingredients, and lipid molecules. Specifically, the composition comprises the following weight percentages:

[0023] Liposomes 0.5%-5%;

[0024] Protein peptide 1.2%-12%;

[0025] Vitamin ingredients 1.01%-4%;

[0026] Hyaluronic acid 0.3%-3%;

[0027] Antioxidant content 1.51%-14%;

[0028] Cell protection ingredients 0.01%-1.5%;

[0029] Lipid molecules 0.51%-4.0%.

[0030] In the skin care composition provided by the present invention, the protein peptide includes: one or more of collagen peptide, elastin peptide, carnosine, and decarboxylated carnosine. The protein peptide is preferably collagen peptide, elastin peptide, carnosine, and decarboxylated carnosine. Among them, the content of collagen peptide is preferably 0.1%-1.0%. The content of elastin peptide is preferably 0.1%-1.0%. The content of carnosine is preferably 0.5%-5%. The content of decarboxylated carnosine is preferably 0.5%-5%.

[0031] In the skin care composition provided by the present invention, the antioxidant ingredients include: one or more of ferulic acid, ergothioneine, bosaiin, and α-arbutin. The antioxidant ingredients are preferably ferulic acid, ergothioneine, bosaiin, and α-arbutin. Among them, the content of ferulic acid is preferably 0.5%-5%. The content of ergothioneine is preferably 0.01%-2.5%. The content of bosaiin is preferably 0.5%-5%. The content of α-arbutin is preferably 0.5%-1.5%.

[0032] In the skin care composition provided by the present invention, the vitamin components include: one or more of tocopherol, ascorbyl palmitate, and retinol palmitate. The vitamin components are preferably tocopherol, ascorbyl palmitate, and retinol palmitate. Among them, the content of tocopherol is preferably 0.5%-2.0%. The content of ascorbyl palmitate is preferably 0.01%-0.5%. The content of retinol palmitate is preferably 0.01%-0.5%.

[0033] In the skin care composition provided by the present invention, the lipid molecules include: one or more of ceramide, lecithin, and cholesterol. The lipid molecules are preferably ceramide, lecithin, and cholesterol. Among them, the content of ceramide is preferably 1%-5%. The content of lecithin is preferably 0.5%-2.0%. The content of cholesterol is preferably 0.01%-2.0%.

[0034] In the skin care composition provided by the present invention, hyaluronic acid includes: one or more of high molecular hyaluronic acid, medium molecular hyaluronic acid, and low molecular hyaluronic acid. Hyaluronic acid is preferably high molecular hyaluronic acid, medium molecular hyaluronic acid, and low molecular hyaluronic acid. Among them, the content of high molecular hyaluronic acid is preferably 0.1%-1.0%. The content of medium molecular hyaluronic acid is preferably 0.1%-1.0%. The content of low molecular hyaluronic acid is preferably 0.1%-1.0%.

[0035] In the skin care composition provided by the present invention, the liposome is 4-butylresorcinol liposome, and the content of 4-butylresorcinol liposome is preferably 0.5%-5%.

[0036] In the skin care composition provided by the present invention, the cell protection component is ectoine, and the content of ectoine is preferably 0.01%-1.5%.

[0037] It should be noted that the percentages involved in this case are all percentages by weight.

[0038] Liposomes have a phospholipid bilayer structure similar to that of cell membranes, and can help active ingredients to penetrate the skin efficiently through active cell endocytosis. In addition, the excellent sustained-release properties of liposomes can effectively control the slow release of active ingredients, reduce cytotoxicity, and increase the duration of action of active ingredients.

[0039] Example 1

[0040] 1. Materials and Reagents

[0041] High molecular weight hyaluronic acid, medium molecular weight hyaluronic acid, low molecular weight hyaluronic acid, collagen peptide, elastin peptide, lecithin, cholesterol, tocopherol, ascorbyl palmitate, and retinol palmitate were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.;

[0042] 4-Butylresorcinol liposomes, ferulic acid, carnosine, decarboxylated carnosine, ergothioneine (2-mercaptohistidine trimethylol salt), bosine (hydroxypropyl tetrahydropyrantriol), α-arbutin, ectoine (2-methyl-1,4,5,6,-tetrahydropyrimidine-4-carboxylic acid), and ceramide were all purchased from Beijing Solebow Technology.

[0043] 2. Instruments and Equipment

[0044] N-1100 rotary evaporator, Shanghai Ailang Instrument Co., Ltd.; SHZ-D (Ⅲ) circulating water multi-purpose vacuum pump, Shanghai Jinfu Experimental Instrument Equipment Co., Ltd.; VC505 cell disruptor, Suzhou Ruinode Biotechnology Co., Ltd.; CF16RN high-speed refrigerated centrifuge, Hitachi (China) Co., Ltd.; SpectraMax microplate reader, Bio-Rad, USA; ZS90 laser nanoparticle size analyzer, Malvern Instruments, UK; JEM2100F transmission electron microscope, JEOL, Japan; TenaorⅡ infrared spectrometer, Bruker, Germany.

[0045] 3. Experimental Methods

[0046] A method for preparing a skin care composition based on liposome technology comprises the following steps:

[0047] Step 1: Divide the raw materials into water-soluble components and fat-soluble components. The water-soluble components are dissolved in water for later use. The fat-soluble components (tocopherol (0.5%-2.0%), ferulic acid (0.5%-5%), ceramide (1%-5%)) are dissolved in ethanol for later use. Lecithin and cholesterol are separately dissolved in ethanol for later use.

[0048] In this step, the water-soluble components are: high molecular hyaluronic acid: 0.1%-1.0%; medium molecular hyaluronic acid: 0.1%-1.0%; low molecular hyaluronic acid: 0.1%-1.0%; collagen peptide: 0.1%-1.0%; elastin peptide: 0.1%-1.0%; ascorbyl palmitate: 0.5%-1.5%; carnosine: 0.5%-5%; decarboxylated carnosine: 0.5%-5%; ergothioneine (2-mercaptohistidine trimethyl inner salt): 0.01%-2.5%; bosine (hydroxypropyl tetrahydropyrantriol): 0.5%-5%; ectoine (2-methyl-1,4,5,6,-tetrahydropyrimidine-4-carboxylic acid): 0.01%-1.5%. The fat-soluble components are: α-arbutin: 0.5%-1.5%; retinol palmitate: 0.01%-0.5%; 4-butylresorcinol liposome: 0.5%-5%.

[0049] Step 2: Preparation of products by thin film hydration

[0050] Take 50 mg of egg yolk lecithin, 8 mg of cholesterol, and 10 μL of Tween-80, add a certain amount of fat-soluble raw materials (tocopherol (0.5%-2.0%), ferulic acid (0.5%-5%), and ceramide (1%-5%)) in the following proportions, and dissolve them in 3 mL of anhydrous ethanol by ultrasonication as the organic phase.

[0051] Weigh 10 mL of dissolved water-soluble raw materials (high molecular weight hyaluronic acid: 0.1%-1.0%; medium molecular weight hyaluronic acid: 0.1%-1.0%; low molecular weight hyaluronic acid: 0.1%-1.0%; collagen peptide: 0.1%-1.0%; elastin peptide: 0.1%-1.0%; ascorbyl palmitate: 0.5%-1.5%; carnosine: 0.5%-5%; decarboxylated carnosine: 0.5%-5%; ergothioneine (2-mercaptohistidine trimethylol salt): 0.01%-2.5%; hydroxypropyl hydroxyethyl ester (hydroxypropyl hydroxyethyl ester)) Tetrahydropyrantriol): 0.5%-5%; Ectoin (2-methyl-1,4,5,6,-tetrahydropyrimidine-4-carboxylic acid): 0.01%-1.5%. The fat-soluble components are: α-arbutin: 0.5%-1.5%; retinol palmitate: 0.01%-0.5%; 4-butylresorcinol liposome: 0.5%-5%) are dissolved in 20mL of 0.01mol / L PBS buffer (phosphate buffered saline), preheated at 50°C, and formed into a uniform vortex by magnetic stirring as the aqueous phase.

[0052] The ethanol in the organic phase was removed by rotary evaporation, so that the lipid formed a uniform film at the bottom of the flask. The aqueous phase was added to the flask, stirred and hydrated for 1 hour, and then ultrasonicated for 12 minutes to obtain a skin care product composition.

[0053] The skin care composition prepared by the method has the characteristics of high encapsulation rate, small particle size and relatively good stability. The average particle size of the skin care composition is (105.42±3.147) nm, the PDI is 0.155±0.019, the particle size distribution range is narrow, and the distribution area is relatively concentrated. The Zeta potential of the skin care composition is (-28.5±0.317) mV. Each active ingredient is encapsulated by the phospholipid bilayer structure of the liposome and reaches the dermis through skin penetration.

[0054] Depend on Figure 1(a) It can be seen that as the amount of egg yolk lecithin added increases, the encapsulation rate of the skin care composition increases accordingly. When the amount of egg yolk lecithin reaches 40 mg, the encapsulation rate of the skin care composition reaches a peak of 69.45%. At this time, if the amount of egg yolk lecithin continues to increase, the encapsulation rate begins to decrease. The phenomenon that the encapsulation increases first and then decreases is mainly because when the content of egg yolk lecithin increases, lipid self-assembly can be promoted, and the effective ingredients encapsulated by the lipid bilayer also increase accordingly; when the amount of egg yolk lecithin is further increased, the symmetry and permeability of the lipid bilayer will be reduced, the overall stability of the system will be weakened, and even lipid aggregation will be caused, resulting in a decrease in the encapsulated efficacy components and a decrease in the encapsulation rate. From the perspective of particle size distribution, when the amount of egg yolk lecithin is 40 mg, the particle size of the prepared skin care composition is relatively small and evenly distributed (PDI = 0.180). Therefore, the amount of egg yolk lecithin should be set to 40 mg.

[0055] Depend on Figure 1 (b) It can be seen that as the proportion of cholesterol usage decreases, the encapsulation of the skin care composition first increases and then decreases, and the corresponding average particle size gradually decreases. When the mass ratio of cholesterol to egg yolk lecithin is 1:4, the encapsulation rate of the skin care composition is the largest and the particle size is moderate. This is because adding a certain amount of cholesterol to the liposome membrane material can adjust the fluidity of the phospholipid bilayer, improve the internal phase leakage phenomenon and increase the stability of the system; cholesterol itself cannot form a bilayer structure, and its excessive usage will make the lipid bilayer too rigid, and the space inside the liposome that can be used to encapsulate SCSP will become smaller, and the encapsulation rate will be reduced; and when the cholesterol ratio is too small, it will affect the formation of the lipid bilayer, causing the lipid membrane to be unstable. Considering the encapsulation rate and particle size distribution, the cholesterol and egg yolk lecithin usage ratio is set to 1:4.

[0056] Depend on Figure 1 (c) It can be seen that as the proportion of the amount of effective ingredients decreases, the encapsulation rate of the skin care composition increases. When the mass ratio of the effective ingredients to the egg yolk lecithin is 1:4, the encapsulation rate of the skin care composition reaches a maximum value of 69.49%. At this time, the proportion of the effective ingredients continues to decrease, and the encapsulation rate of the skin care composition is significantly reduced. This is because the SCSP dosage is too high, which will result in most of the effective ingredients still being present in the aqueous buffer in a free form when the encapsulation capacity reaches the maximum under this lipid dosage, and the effective ingredients are wasted, and the encapsulation rate is relatively low; and the less amount of effective ingredients will lead to uneven liposome encapsulation, imbalance of the entire system, and reduced encapsulation rate. The change in the amount of effective ingredients has little effect on the particle size of the skin care composition. Therefore, the mass ratio of the effective ingredients (the above-mentioned fat-soluble and water-soluble ingredients) to the egg yolk lecithin is set to 1:4.

[0057] This study shows that the optimal conditions for the preparation of skin care compositions are: 40 mg of egg yolk lecithin, a mass ratio of cholesterol to egg yolk lecithin of 1:4, a mass ratio of effective ingredients to egg yolk lecithin of 1:4, 10 μL of Tween-80, 10 mL of PBS buffer, 1 hour of hydration, and 12 minutes of probe ultrasound. Three batches of skin care compositions were prepared according to the optimized conditions of this experiment, and the particle sizes were measured to be 108.6 nm, 105.3 nm, and 107.6 nm, with an average particle size of (107.2 ± 1.4) nm; the encapsulation efficiency was 88.17%, 82.54%, and 85.24%, with an average encapsulation efficiency of 85.32% ± 1.24%. The three batches of skin care compositions were stable in nature.

[0058] The multifunctional skin care product based on liposome technology provided by the present invention can achieve multiple functions of moisturizing, repairing, whitening and anti-aging compared with the prior art, and meet various skin care needs. Through the liposome encapsulation technology, various active ingredients can be effectively encapsulated, their stability and permeability can be improved, and the skin care effect can be enhanced. Natural active ingredients are used to reduce skin irritation and side effects.

[0059] 4. Experimental Data

[0060] Subjects:

[0061] A total of 33 subjects who met the test criteria were screened, of whom 1 withdrew from the trial due to personal reasons. A total of 32 people actually completed the test, with an age range of 28 to 56 years and an average age of 46.3±6.9 years.

[0062] Test method:

[0063] Refer to "Human Evaluation Method for the Repair Effect of Cosmetics" TGDCA009-2022, "Technical Specifications for Cosmetic Safety (2022 Edition)" - Human Open Use Freckle Removal and Whitening Effect Test Method, T / CAB 0152-2022 "Seven Test Methods for Cosmetic Anti-wrinkle, Firming, Moisturizing, Oil Control, Repair, Nourishing, and Soothing Effects", evaluate the following indicators, and detect the moisturizing, repairing, whitening, and anti-aging effects of the prepared multifunctional liposomes.

[0064] Test Results

[0065] Moisturizing and repairing efficacy test results

[0066] The facial feeling rating of the subjects after using the product for the first time

[0067] The results of the facial sensation ratings of the subjects after using the product for the first time under the supervision of laboratory personnel are shown in Table 1 below.

[0068] Table 1 Product perception ratings after first use

[0069]

[0070]

[0071] Rating criteria: Burning score: 0 points: None; 1 point: Mild, occasionally felt; 2 points: Moderate, obvious burning, but tolerable; 3 points: Severe, very obvious burning, need to use cold water to relieve. Stinging score: 0 points: None; 1 point: Mild, occasionally felt; 2 points: Moderate, obvious stinging, but tolerable; 3 points: Severe, very obvious stinging, need external medication to relieve. Itch score: 1 point (0 degree): basically no itching; 2 points (mild, degree I): slight itching, no need to scratch, can be relieved by stroking and patting, no secondary rash caused by scratching; 3 points (moderate, degree II): obvious itching, can't help scratching, mild secondary rash caused by scratching can be seen; 4 points and above (severe, degree III and extremely severe, degree IV): severe is severe itching, affecting sleep and work, obvious secondary rash caused by scratching can be seen locally, extremely severe is severe itching, affecting sleep and work, emotional distress, and secondary rashes are severe and numerous.

[0072] Skin reactions during product use by test subjects

[0073] The results of the laboratory personnel's clinical evaluation of the subjects' adverse skin reactions during product use are shown in Table 2 below, and the criteria for adverse skin reactions are shown in Table 3.

[0074] Table 2 Summary of cosmetics sensitive skin applicability test results

[0075]

[0076] Table 3 Standards for adverse skin reactions

[0077]

[0078] Measurement results of transepidermal water loss TEWL values ​​at different time points after use of the test substance

[0079] After using the product for 4 weeks, the subjects' facial TEWL values ​​were significantly lower than before use. The results are shown in Tables 4 and 5.

[0080] Table 4 Transepidermal water loss TEWL values ​​at different time points after use of the test substances (g / m 2 / h)Results

[0081]

[0082] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0083] Table 5 Difference in transepidermal water loss rate (TEWL) at different time points after use of the test substances (g / m 2 / h)Results

[0084]

[0085] Results of skin hemoglobin index at different time points after using the test substance

[0086] After using the product for 4 weeks, the hemoglobin index of the subjects' facial skin was significantly lower than that before use. The results are shown in Tables 6 and 7.

[0087] Table 6 Results of skin hemoglobin index at different time points after use of the test substances

[0088]

[0089]

[0090] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0091] Table 7 Results of skin hemoglobin index difference at different time points after using the test substances

[0092]

[0093] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0094] At different time points after the test substance was used * Value measurement results

[0095] After using the product for 4 weeks, the subjects' faces * The values ​​were significantly lower than before use. The results are shown in Table 8 and Table 9.

[0096] Table 8 Different time points after the use of the test substances * Value Result

[0097]

[0098] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0099] Table 9 Different time points after the use of the test substances * result

[0100]

[0101] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0102] Results of skin moisture content at different time points after using the test substance

[0103] After using the product for 2 and 4 weeks, the moisture content of the subjects' facial stratum corneum increased significantly compared to before use. The results are shown in Tables 10 and 11.

[0104] Table 10 Results of skin stratum corneum water content (CU) at different time points after using the test substances

[0105]

[0106] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0107] Table 11 Results of skin stratum corneum moisture difference (CU) at different time points after using the test substance

[0108]

[0109] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0110] Results of the test questionnaire used by the subjects

[0111] After using the product, the subjects evaluated the product through a consumer questionnaire. The following is the survey content of the questionnaire:

[0112] 7.1 Issues with product claims of “mild (non-irritating)” and “suitable for sensitive skin”

[0113] During the period of product use by the subjects, all subjects had no adverse reactions or discomfort. The results are shown in Table 12 below.

[0114] Table 12 Survey results on the subjects' opinions on the products being "mild" and "suitable for sensitive skin"

[0115]

[0116] 1.8 Issues with product “repair” efficacy claims

[0117] After using the product for 4 weeks, the subjects completed a questionnaire evaluation on the improvement effect of the product. The results of the test questionnaire are summarized in Table 13 below.

[0118] Table 13 Summary of the results of the consumer usage test questionnaire survey

[0119]

[0120]

[0121] Results of a trial on whitening and skin tone improvement

[0122] Results of skin moisture content at different time points after using the test substance

[0123] After using the product for 2 and 4 weeks, the moisture content of the subjects' facial stratum corneum increased significantly compared to before use. The results are shown in Tables 1 and 2.

[0124] Table 1 Results of skin stratum corneum water content (CU) at different time points after using the test substances

[0125]

[0126] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0127] Table 2 Results of skin stratum corneum moisture difference (CU) at different time points after using the test substances

[0128]

[0129] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0130] L at different time points after the test substance was used * Value measurement results

[0131] After using the product for 4 weeks, the subjects' facial L * The values ​​were significantly higher than those before use. The results are shown in Table 3 and Table 4.

[0132] Table 3 L at different time points after use of the test substances * Value Result

[0133]

[0134] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0135] Table 4 L at different time points after use of the test substances * result

[0136]

[0137] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0138] Color ITA° value

[0139] After using the product for 4 weeks, the skin color of the subjects' cheeks was analyzed. The results showed that the ITA° value index of the subjects' skin color had improved significantly compared with before use. The results are shown in Tables 5 and 6.

[0140] Table 5 Results of lightness index ITA° values ​​at different time points after use of the test substances

[0141]

[0142]

[0143] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0144] Table 6 ITA° difference results at different time points after use of the test substances

[0145]

[0146] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0147] Results of skin melanin index at different time points after using the test substance

[0148] After using the product for 4 weeks, the hemoglobin index of the subjects' facial skin was significantly lower than that before use. The results are shown in Tables 7 and 8.

[0149] Table 7 Results of skin melanin index at different time points after use of the test substances

[0150]

[0151] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0152] Table 8 Melanin index difference results at different time points after use of the test substances

[0153]

[0154] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0155] Gloss results of the test article at different time points after use

[0156] After using the product for 4 weeks, the glossiness of the subjects' cheeks was analyzed. The results showed that the glossiness of the subjects' faces was significantly increased compared to before use. The results are shown in Tables 9 and 10.

[0157] Table 9 Gloss results of the test objects at different time points after use

[0158]

[0159] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0160] Gloss difference results of the test object at different time points after use

[0161]

[0162] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0163] Results of the test questionnaire used by the subjects

[0164] After using the product for 4 weeks, the subjects completed a questionnaire evaluation on the improvement effect of the product. The results of the test questionnaire are summarized in Table 11 below.

[0165] Table 11 Summary of the results of the consumer usage test questionnaire survey

[0166]

[0167] Anti-wrinkle, anti-aging, and skin firming efficacy test results

[0168] Results of improvement of eye wrinkles at different time points after using the test substance

[0169] After using the product for 2 and 4 weeks, the wrinkles in the fixed selected area around the corners of the eyes of the subjects were analyzed. The results showed that the wrinkles in the corners of the eyes of the subjects in the selected area were significantly improved before and after using the product. The results are shown in Tables 1 and 2.

[0170] Table 1 Results of the length, average width and average depth of eye wrinkles at different time points after using the test substances

[0171]

[0172]

[0173]

[0174] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0175] Table 2 The difference of eye wrinkles at different time points after using the test substances

[0176]

[0177] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0178] Elasticity and firmness results at different time points after using the test substance

[0179] Elasticity parameter R2 results

[0180] After using the product for 2 and 4 weeks, the skin elasticity of the subjects' cheeks was analyzed. The results showed that the subjects' facial elasticity parameter R2 was significantly increased compared with before use. The results are shown in Tables 3 and 4.

[0181] Table 3 Elasticity parameter R2 results at different time points after use of the test objects

[0182]

[0183]

[0184] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0185] Table 4 Results of R2 difference of elastic parameters at different time points after use of the test objects

[0186]

[0187] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0188] Compact parameter F4 results

[0189] After using the product for 4 weeks, the skin firmness of the subjects' cheeks was analyzed. The results showed that the facial firmness parameter F4 of the subjects was significantly reduced compared with before use. The results are shown in Table 5 and Table 6

[0190] Table 5 Results of the firming parameter F4 at different time points after use of the test substances

[0191]

[0192]

[0193] Note: 1) Normality test p value>0.05 indicates that the results follow normal distribution, and paired T test is used. If it does not follow normal distribution, non-parametric rank sum test is used. 2) Compared with the baseline value before use in the nth week, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0194] Table 6 The difference results of the compaction parameter F4 at different time points after the use of the test substances

[0195]

[0196] Note: p value, "ns" indicates no statistical difference, p>0.05; "*" indicates significant difference, 0.01≤p<0.05; "**" indicates very significant difference, 0.001≤p<0.01; "***" indicates extremely significant difference, p<0.001.

[0197] Results of the test questionnaire used by the subjects

[0198] After using the product for 4 weeks, the subjects completed a questionnaire evaluation on the improvement effect of the product. The summary results using the test questionnaire are shown in Table 7 below.

[0199] Table 7 Summary of the results of the consumer use test questionnaire survey

[0200]

[0201]

[0202] V. Results Analysis

[0203] Analysis of moisturizing and repairing effects

[0204] The clinical evaluation results showed that none of the 30 subjects experienced any adverse reactions after use, and the self-evaluation results of the subjects showed that they did not feel any discomfort during or after use. The test results showed that the Huasikang Liposome Rejuvenating Mask has a high degree of safety, and the product is "mild (non-irritating)", meeting the claim of "suitable for sensitive skin".

[0205] After using the product for 2 weeks, the subjects' skin transepidermal water loss rate TEWL value decreased by 4.97%, which was not significantly different from before use, and the skin hemoglobin index decreased by 2.64%, which was not significantly different from before use. *The value decreased by 8.66%, which was not significantly different from before use. The water content of the stratum corneum increased by 5.63% (p<0.05), which was significantly different from before use. After 4 weeks of use, the TEWL value of the skin decreased by 12.90% (p<0.001), which was significantly different from before use. The skin hemoglobin index decreased by 7.15% (p<0.05), which was significantly different from before use. * The value decreased by 14.32% (p<0.01), which was significantly different from before use. The water content of the stratum corneum increased by 11.12% (p<0.01), which was significantly different from before use. According to the standards of "Human Evaluation Method for Cosmetic Repair Effect" TGDCA009-2022 and "Test Method for Seven Efficacy of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repair, Nourishing, and Soothing" T / CAB0152-2022, the above results show that the product has a repair effect.

[0206] Through this consumer questionnaire test, after 4 weeks of product use, 96.7% of the subjects believed that "skin is more moisturized after using the product", 93.3% of the subjects believed that "skin condition is stabilized after using the product", 96.7% of the subjects believed that "skin redness is improved after using the product", 96.7% of the subjects believed that "the stinging, burning, tightness and other discomforts caused by dryness are improved after using the product", 93.3% of the subjects believed that "skin barrier is stronger after using the product", 93.3% of the subjects believed that "skin is brighter and more shiny after using the product", 96.7% of the subjects believed that "skin is softer after using the product", and 96.7% of the subjects believed that "skin is more delicate and smoother after using the product", and the results showed significant differences, which supports the product's claim of "repair" effect.

[0207] During the use of the test substance, none of the 30 subjects experienced any adverse skin reactions, and 2 subjects withdrew from the trial due to personal reasons and were unable to return for follow-up visits on time.

[0208] Analysis of the results of whitening and skin tone improvement

[0209] After using the product for 2 weeks, the water content of the stratum corneum of the subjects' skin increased by 8.47% (p<0.01), which was a very significant difference compared with before use. * The value increased by 0.84%, which was not significantly different from before use. The ITA° value increased by 2.25%, which was not significantly different from before use. The melanin value of the skin decreased by 2.41%, which was not significantly different from before use. The gloss value increased by 2.60%, which was not significantly different from before use.

[0210] After using the product for 4 weeks, the water content of the stratum corneum of the subjects' skin increased by 13.74% (p<0.001), which was a very significant difference compared with before use. * The value increased by 4.11% (p<0.05), which was significantly different from before use. The ITA° value increased by 8.85% (p<0.05), which was significantly different from before use. The melanin value of the skin decreased by 3.69% (p<0.05), which was significantly different from before use. The gloss value increased by 5.97% (p<0.05), which was significantly different from before use. Referring to the "Human Evaluation Method for Cosmetic Repair Efficacy" TGDCA009-2022, "Cosmetic Safety Technical Specifications (2022 Edition)"-Human Open Use Freckle Removal and Whitening Efficacy Test Method, "Cosmetic Anti-wrinkle, Firming, Moisturizing, Oil Control, Repair, Nourishing, Soothing Seven Efficacy Test Methods" T / CAB 0152-2022 standard, the above results show that the skin glossiness is improved after use of the product, and the product has a moisturizing effect.

[0211] Through this consumer questionnaire test, after 4 weeks of product use, 96.9% of the subjects believed that "after using the product, the skin's radiance is improved and the skin tone is effectively brightened", 96.9% of the subjects believed that "after using the product, the dull skin is effectively improved", 93.8% of the subjects believed that "after using the product, the sallow skin is effectively improved", 90.6% of the subjects believed that "after using the product, the skin tone is even, and dark spots are effectively suppressed", 96.9% of the subjects believed that "after using the product, the skin becomes hydrated and bright, and 93.8% of the subjects believed that "after using the product, the skin becomes even and fair", and the results showed significant differences.

[0212] During the use of the test substance, none of the 32 subjects experienced any adverse skin reactions, and one subject withdrew from the trial due to personal reasons and failure to return for a follow-up visit on time.

[0213] Analysis of anti-wrinkle, anti-aging and skin tightening efficacy results

[0214] After the subjects used the product for 2 weeks, the length of the wrinkles at the corners of the eyes decreased by 4.57% (p<0.05) compared with before use, which was significantly different from before use. The average width of the wrinkles at the corners of the eyes decreased by 10.72% (p<0.05), which was significantly different from before use. The average depth of the wrinkles at the corners of the eyes decreased by 10.22% (p<0.001), which was extremely significantly different from before use. After the subjects used the product for 4 weeks, the length of the wrinkles at the corners of the eyes decreased by 14.40% (p<0.01) compared with before use, which was very significantly different from before use. The average width of the wrinkles at the corners of the eyes decreased by 15.03% (p<0.01), which was very significantly different from before use. The average depth of the wrinkles at the corners of the eyes decreased by 16.37% (p<0.001), which was extremely significantly different from before use. The above results show that the product has anti-wrinkle effects.

[0215] After using the product for 2 weeks, the skin firmness F4 value decreased by 4.48%, and the elasticity index R2 value increased by 2.38% (p<0.05), which was significantly different from before use. After using the product for 4 weeks, the skin firmness F4 value decreased by 12.22% (p<0.001), which was extremely significantly different from before use, and the elasticity index R2 value increased by 8.43% (p<0.001), which was extremely significantly different from before use. The above results show that the product has a firming effect.

[0216] Through this consumer questionnaire test, after 4 weeks of product use, 93.3% of the subjects believed that "after using the product, the skin is more delicate and smooth", 93.3% of the subjects believed that "after using the product, the skin feels fuller and plumper", 93.3% of the subjects believed that "after using the product, facial dry lines and fine lines are faded", 96.7% of the subjects believed that "after using the product, facial wrinkles are improved", 93.3% of the subjects believed that "after using the product, the contour is lifted and tightened", 96.7% of the subjects believed that "after using the product, the skin firmness is improved", 93.3% of the subjects believed that "after using the product, the skin elasticity is improved", and 93.3% of the subjects believed that "after using the product, the skin sagging is improved", and the results showed significant differences, which supports the product's claim of "anti-wrinkle and firming" efficacy.

[0217] During the use of the test substance, none of the 30 subjects experienced any adverse skin reactions, and 2 subjects withdrew from the trial due to personal reasons and were unable to return for follow-up on time.

[0218] Appendix 1: Subject Information Form

[0219]

[0220]

[0221] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin care composition based on liposome technology, characterized in that: The invention comprises the following components in weight percentage: 0.5%-5% liposome; 1.2%-12% protein peptide; 1.01%-4% vitamin component; 0.3%-3% hyaluronic acid; 1.51%-14% antioxidant component; 0.01%-1.5% cell protection component; and 0.51%-4.0% lipid molecule.

2. A skin care composition based on liposome technology as claimed in claim 1, characterized in that: The protein peptides include: one or more of collagen peptides, elastin peptides, carnosine, and decarboxylated carnosine.

3. A skin care composition based on liposome technology as claimed in claim 1, characterized in that: The antioxidant components include: one or more of ferulic acid, ergothioneine, boraxine, and α-arbutin.

4. A skin care composition based on liposome technology as claimed in claim 1, characterized in that: The vitamin components include: one or more of tocopherol, ascorbyl palmitate, and retinol palmitate.

5. A skin care composition based on liposome technology as claimed in claim 1, characterized in that: The lipid molecules include: one or more of ceramide, phosphatidylcholine and cholesterol.

6. A skin care composition based on liposome technology as claimed in claim 1, characterized in that: The liposome is 4-butylresorcinol liposome.

7. A skin care composition based on liposome technology as claimed in claim 3, characterized in that: The antioxidant components are ferulic acid, ergothioneine, bosine and α-arbutin; wherein the content of ferulic acid is 0.5%-5%; the content of ergothioneine is 0.01%-2.5%; the content of bosine is 0.5%-5%; and the content of α-arbutin is 0.5%-1.5%.

8. The skin care composition based on liposome technology as claimed in claim 4, characterized in that: The vitamin components include tocopherol, ascorbyl palmitate and retinol palmitate; the content of the tocopherol is 0.5%-2.0%; the content of the ascorbyl palmitate is 0.01%-0.5%; and the content of the retinol palmitate is 0.01%-0.5%.

9. A method for preparing the skin care composition based on liposome technology according to claim 1, characterized in that: include: The raw materials are divided into water-soluble components and fat-soluble components; the water-soluble components are dissolved in water for later use; the fat-soluble components are dissolved in ethanol for later use; The products were prepared by thin film hydration method.

10. An essence milk, characterized in that: A skin care composition based on liposome technology comprising any one of claims 1 to 8 or a skin care composition based on liposome technology prepared by the preparation method of claim 9.

Images