Skin barrier repairing composition and application thereof in cosmetics

Abstract

The invention discloses a skin barrier repairing composition and application thereof in cosmetics. The silanized hyaluronate and the capparis spinosa fruit composite liquid are compounded to prepare the composition, and synthesis of ZO-1 and TGM-5 proteins can be remarkably promoted, so that the effect of effectively repairing the skin barrier is achieved; then the composition is used as a main effective component to prepare a skin care cosmetic, and tests prove that the skin care cosmetic has good effects of improving the problems of dry skin, redness and barrier damage.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a skin barrier repairing composition and application thereof in cosmetics. Background Art

[0002] Skin barrier repair plays a vital role in maintaining the health and normal function of the skin. A healthy skin barrier can resist the entry of harmful external irritants and has moisturizing and anti-inflammatory effects. Damage to the skin barrier will cause problems such as dry skin, skin aging, pigmentation, etc. In severe cases, it can cause skin problems such as atopic dermatitis, eczema, psoriasis, ichthyosis, solar dermatitis, etc.

[0003] Epidermal tight junctions (TJs) are essential functional structures of the skin barrier. They exist in almost all epidermis and are considered to be the main control factors of the epidermal cell barrier system. Abnormal tight junctions can lead to damaged skin barriers. Various proteins such as occludin, Claudin protein, pericytin family (ZO protein), and junctional adhesion molecules are involved in the formation of tight junctions. Tight junction proteins interact to form TJ complexes. The protein complex connects cells to each other or connects cells to the extracellular matrix. ZO-1 protein directly binds to actin, thus forming a stable connection between the cytoskeleton and the TJ complex. The structural stability of tight junctions reflects the integrity of the barrier function. Damage to ZO-1 protein can lead to loose intercellular connections, cell dispersion or even shedding, increased barrier permeability, and escape of fluids, cells or cytokines in the barrier, thereby causing damage to the body. Therefore, ZO-1, as a skeleton protein of tight junctions, plays an important role in maintaining the structural stability of the skin barrier, the integrity of the tight junction layer, and the normal permeability function of the epidermis.

[0004] Keratinocytes are cells that make up most of the epidermis. Filaggrin cross-links with keratin filaments to provide strength and structure. The proteins of the keratinocyte membrane (FLG, IVL, LOR, KRT) are cross-linked in this layer by the calcium-requiring enzyme transglutaminase to form a cornified capsule. The cornified capsule determines the biomechanical properties of keratinocytes and provides firmness for keratinocytes. Transglutaminase 5 (TGM-5) is a key differentiation protein for keratinocytes and an important component of the cornified capsule. It can maintain barrier health by regulating proteins such as FLG, IVL, and LOR in the cornified capsule. TGM-5 is mainly distributed in the granular layer in normal epidermis, and its expression is upregulated. It can catalyze the cross-linking of the above structural proteins to induce epidermal keratinization. When TGM-5 decreases, it will affect the formation of the cornified capsule and the differentiation of keratinocytes, and a complete skin barrier cannot be formed. Only when a precise balance is achieved between the normal gene-encoded expression of the cornified capsule protein and TGM-5, can the epidermis form normally and form keratin.

[0005] A healthy skin barrier is the basis of many important physiological functions of the skin. Tight junctions and cornified capsules are important structures for maintaining a healthy skin barrier. Therefore, increasing ZO-1 and TGM-5 is of great significance for repairing the health of the skin barrier. Based on this, the applicant has developed a composition for repairing the skin barrier by increasing ZO-1 and TGM-5. Summary of the invention

[0006] In view of the above problems, the present invention provides a skin barrier repairing composition and its application in cosmetics. The present invention prepares a composition by compounding silanized hyaluronic acid ester and caper fruit composite liquid, which can significantly promote the synthesis of ZO-1 and TGM-5 proteins; then the composition is used as the main active ingredient to prepare skin care cosmetics, which has good effects on improving dry skin, redness, and barrier damage, and therefore has good application prospects in the field of cosmetics.

[0007] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0008] The first aspect of the present invention provides a skin barrier repairing composition, comprising the following components in parts by weight:

[0009] 0.01-0.1 parts of silanized hyaluronic acid ester (average molecular weight 700-800 kDa), and 0.5-10.0 parts of caper fruit composite liquid.

[0010] Furthermore, the skin barrier repairing composition comprises the following components in parts by weight:

[0011] 0.03-0.08 parts of silanized hyaluronic acid ester (average molecular weight 700-800 kDa), 1.0-8.0 parts of caper fruit composite liquid.

[0012] Preparation method: dissolve the silanized hyaluronic acid ester and the caper fruit composite liquid in water (make up to 100 parts).

[0013] Among them, the silanized hyaluronic acid ester refers to a compound in which dimethylsilane monool or dimethylsilane diol is ester-bonded with hyaluronic acid, which can be obtained by a publicly known preparation method, such as reacting chlorosilane with hyaluronic acid suspended in an organic solution and then precipitating to obtain a solid substance (CN103450368A), which has good moisturizing, prevention and repair of skin damage and other effects.

[0014] The preparation method of the caper fruit composite liquid is specifically as follows: adding water to caper fruit powder and stirring and extracting for 1-3 times at 60-90° C., and combining the filtrate; then adding a composite enzyme consisting of α-amylase, acid protease and neutral protease for enzymolysis to obtain a crude extract, and filtering; then adding polyamide resin and activated carbon for adsorption, and then adding glycerol and mixing evenly to obtain the caper fruit composite liquid (3.5-4.0 times the mass of the caper fruit powder).

[0015] Preferably, the preparation method comprises the following steps:

[0016] S1: Weigh the crushed and sieved (60-100 mesh) caper fruit powder, add water, stir and extract at 60-90°C for 1.5-3.5h, filter to obtain a filtrate, add water to the filter residue, stir and extract at 60-90°C for 1.5-3.25h, filter to obtain a filtrate, and combine the two filtrates;

[0017] S2: adding 0.2-0.4% (relative to the weight of the caper fruit powder) of a composite enzyme consisting of α-amylase, acid protease and neutral protease to the filtrate, wherein the mass ratio of the α-amylase, acid protease and neutral protease is 1:0.8-1.2:0.8-1.2, stirring at 40-60° C. for 0.5-2 h, and then inactivating the enzyme to obtain a crude extract;

[0018] S3: filtering the crude extract, adding polyamide resin and activated carbon to the filtrate (the mass of the polyamide resin is 0.1-1% of the mass of the caper fruit powder, and the amount of activated carbon is 0.1-0.7% of the mass of the caper fruit powder), stirring and adsorbing at room temperature for 1-1.5 hours, filtering, and obtaining a caper fruit extract;

[0019] S4: The caper fruit extract and glycerin are mixed and stirred evenly to obtain the caper fruit composite liquid.

[0020] Capparis spinosa L. is a perennial vine and semi-shrub. Its main functions include dispelling wind and dampness, relieving pain, and reducing swelling. It can be applied to the affected area to treat rheumatoid arthritis and sores. Current research shows that the fruit extract of Capparis spinosa contains a variety of functional ingredients such as flavonoids and alkaloids, which have multiple functions such as anti-inflammatory, analgesic and antioxidant effects. It can be used in cosmetics to regulate the skin.

[0021] The second aspect of the present invention provides the use of the above-mentioned skin barrier repairing composition in the preparation of cosmetics for repairing the skin barrier and improving the problems of dry skin and redness. The present invention verifies through experiments that the above-mentioned composition can improve the problems of dry skin and redness by promoting the expression of ZO-1 and TGM-5, and can be used in cosmetics to repair the skin barrier and improve the problems of dry skin and redness. The cosmetics can be skin care products.

[0022] The third aspect of the present invention provides a cosmetic for repairing skin barrier, wherein the cosmetic at least comprises the above-mentioned skin barrier repairing composition.

[0023] Wherein, based on the total mass of the cosmetic, the added amount of the skin barrier repairing composition is 0.1-10%, preferably 1-8%.

[0024] The cosmetics may also contain any other raw material ingredients permitted to be added in the cosmetics field, including but not limited to emulsifiers, emollients, moisturizers, thickeners, preservatives, and the like.

[0025] At the same time, the present invention can also prepare different cosmetic dosage forms, such as essence water, essence lotion, essence cream, etc., by reasonably adding the above-mentioned raw material ingredients. At the same time, based on the above-mentioned basic cosmetic categories, other cosmetic categories are further derived and prepared, which obviously belong to the protection scope of this application.

[0026] The fourth aspect of the present invention provides a method for preparing the above-mentioned cosmetics, the method comprising: mixing the skin barrier repairing composition with other raw material components, without specific limitation herein.

[0027] The technical effects of the present invention are:

[0028] (1) The skin barrier repairing composition obtained by the present invention can significantly promote the synthesis of ZO-1 and TGM-5 proteins, thereby achieving the effect of effectively repairing the skin barrier;

[0029] (2) Silylated hyaluronic acid ester has a weak effect on promoting the expression of ZO-1 and TGM-5, especially for the expression of ZO-1. The present invention uses silanized hyaluronic acid ester in combination with a composite solution of Capparis spinosa fruit (water heating extraction + enzymatic hydrolysis). The composition can more significantly promote the expression of ZO-1 and TGM-5 proteins, expanding the function and application of silanized hyaluronic acid ester in repairing the skin barrier.

[0030] (3) The test results show that the caper fruit complex solution (water heating extraction + enzymatic hydrolysis) has better skin care effect than the caper fruit complex solution (65% ethanol ultrasonic extraction), thus making the product more effective and more cost-effective.

[0031] (4) The preparation method of the skin barrier repairing composition skin care cosmetics prepared by the present invention is simple and easy, saves costs, and has good effects on improving skin dryness and redness through human efficacy experiments, so it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 : The facial red area change diagram of the subject No. 2 in Example 5 before and after using the sample (D0, D14 and D28);

[0033] Figure 2 : Changes in facial red area of ​​subject No. 13 of Example 5 before and after using the sample (D0, D14 and D28). DETAILED DESCRIPTION

[0034] The present invention is further described in conjunction with specific examples. The following examples are only for explaining the present invention and are not intended to limit its contents. If the specific experimental conditions are not specified in the examples, they are usually in accordance with conventional conditions or the conditions recommended by the reagent company; the reagents, consumables, etc. used in the following examples can be obtained from commercial sources unless otherwise specified. All technical and scientific terms used herein have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0035] It should be noted that the silanized hyaluronic acid ester in the embodiment of the present invention is a solid composite obtained by reacting chlorosilane with hyaluronic acid suspended in an organic solution and then precipitating (CN103450368A). The average molecular weight of silanized hyaluronic acid ester is 700-800 kDa. Sodium hyaluronate (average molecular weight 700-800 kDa) in the comparative example was purchased from Shandong Baifu Furuida Pharmaceutical Co., Ltd., trade name: sodium hyaluronate, INCI name: sodium hyaluronate.

[0036] The Capparis spinosa fruit composite liquid (method 1) in the examples and comparative examples of the present invention is obtained by the following method:

[0037] S1: Weigh the crushed and sieved (80 mesh) caper fruit powder and mix it with water at a solid-liquid ratio of 1:5 (mass ratio), stir and extract at 80°C and 70rpm for 2h, filter to obtain a filtrate, add water to the residue again at a solid-liquid ratio of 1:4 (mass ratio), stir and extract at 80°C and 70rpm for 2h, filter to obtain the filtrate, and combine the two filtrates;

[0038] S2: Add 0.3% (relative to the weight of the caper fruit powder) of a composite enzyme consisting of α-amylase, acid protease and neutral protease to the filtrate, wherein the mass ratio of the α-amylase (model 3000u / mL, product batch number MY20011001, from Ningxia Xiasheng Biology), the acid protease (model 100,000u / g, product batch number 2191135, from Ningxia Xiasheng Biology) and the neutral protease (model 100,000u / g, product batch number F181070, from Ningxia Xiasheng Biology) is 1:1:1, and the mixture is enzymolyzed at 45°C for 1.5h with stirring, and the enzyme is inactivated at 90°C for 10min to obtain a crude extract;

[0039] S3: Filter the crude extract with a 10 μm polypropylene membrane to obtain a filtrate, add polyamide resin and activated carbon to the filtrate, the mass of the polyamide resin is 0.3% of the mass of the caper fruit powder, and the amount of activated carbon is 0.4% of the mass of the caper fruit powder. Stir and adsorb at room temperature for 1.5 hours, filter with a 0.22 μm polypropylene membrane to obtain a caper fruit extract, the mass of which is 3 times that of the caper fruit powder;

[0040] S4: The caper fruit extract and glycerin are mixed and stirred evenly in a volume ratio of 4:1 to obtain a caper fruit composite liquid.

[0041] The caper fruit composite liquid (method 2) in the examples and comparative examples of the present invention is prepared by the following method:

[0042] S1: The caper fruit powder was sieved (80 mesh sieve was used), 65% ethanol solution was used as the extraction solvent, the solid-liquid ratio was 1:20 (g / mL), ultrasonic extraction was performed, the ultrasonic power was 150W, the processing temperature was 35°C, and the processing time was 8min;

[0043] S2: The extract is subjected to reduced pressure distillation at 55° C. to be concentrated to 3 times the mass of the caper fruit powder, glycerol is added, and the concentrate and glycerol are mixed and stirred evenly in a volume ratio of 4:1.

[0044] Examples 1-3 and Comparative Examples 1-8:

[0045] The specific scheme is shown in Table 1 below, calculated by mass fraction. The preparation method is: according to the ratio described in Table 1, the raw materials are mixed together and dissolved in purified water.

[0046] Table 1 Specific examples and comparative examples ingredient list

[0047]

[0048] Example 5: Skin barrier repairing cream

[0049] The above composition is used to prepare an essence cream product containing the skin barrier repairing composition, and the formula is shown in Table 2.

[0050] Table 2 Essence cream product formula

[0051]

[0052] Preparation method of essence cream:

[0053] S1: Preparation of aqueous phase: Add the raw materials in phase A respectively, heat to 80-85°C and stir to dissolve;

[0054] S2: Preparation of oil phase: Add the raw materials in phase B respectively and heat to 75-80°C to melt;

[0055] S3: Combine phase A and phase B, start homogenization (3000 rpm), homogenize for 12 min, and start cooling;

[0056] S4: When the temperature drops to 65-70°C, add phase C, stir to dissolve, and continue to cool after dissolution;

[0057] S5: When the temperature drops to 40-45°C, add phase D ingredients, stir to dissolve and disperse evenly to obtain the essence cream product.

[0058] Experimental Example 1: Experiment on promoting the expression of ZO-1 and TGM-5 proteins

[0059] The experiment used human keratinocytes (HaCat) as a model cell line. The cells were inoculated into 96-well plates and incubated overnight in a 37°C, 5% CO2 incubator. Each plate was set up with NC group, SLS group and sample group. When the cell plating rate in the 96-well plate reached 40% to 60%, grouping and drug administration were carried out, with 3 replicate wells in each group.

[0060] 1. Preparation of cell plates: HaCat cells in logarithmic phase were added to 96-well plates and cultured overnight in a 37° C., 5% CO 2 incubator to allow the cells to adhere to the plate.

[0061] 2. SLS (sodium dodecyl sulfate) treatment: Prepare samples separately using complete culture medium (Source Culture Biology), as shown in Table 3. Add 100 μL of the sample to be tested or complete culture medium to the 96-well plate as shown in Table 3, and then add 100 μL of 60 μg / mL SLS solution (prepared with complete culture medium) to each well, of which only 200 μL of complete culture medium was added to the NC group, the final concentration of the SLS group sample was 30 μg / mL SLS, and the final concentration of the sample group was 5 mg / mL of the sample to be tested + 30 μg / mL SLS. Mix well and continue to culture in a constant temperature incubator at 37°C and 5% CO2 for 24 hours. Each cell plate is set up with NC group, SLS group and sample group, and each group has at least 3 replicates.

[0062] 3. Immunofluorescence staining: 50 μL of TGM-5 and ZO-1 antibody solutions (Abcam; 1:200 dilution) were added respectively, incubated at 4°C overnight, and then 50 μL of fluorescent secondary antibody solution (Invitrigen; 1:1000 dilution) was added. Incubated at room temperature in the dark for 1 hour, washed, 100 μL of nuclear dye DAPI (Invitrigen; 1:1000 dilution) was added to each well for staining for 5 minutes, and then 100 μL of PBS was added and photographed under a fluorescence microscope. Three typical photos were selected for each group, analyzed using Image J, and RFU (relative fluorescence unit) was obtained.

[0063] Calculation formula: Calculate the change rate of the sample group with RFU, and set the SLS group as 100%. The calculation formula is as follows:

[0064] Rate of change (%) = (RFU 样品组 / RFU SLS组 -1)×100%

[0065] 4. Data analysis: The quantitative data were expressed as mean ± standard deviation. The t-test was used to calculate the significant difference, where *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001, respectively.

[0066] Table 3 Test plan

[0067]

[0068]

[0069] Table 4 Summary of ZO-1 protein content test results

[0070] Sample name Average RFU SD P-value Upregulation rate (%, vs SLS group) NC Group 20.009 1.881 / / SLS Group 2.411 0.293 / / Example 1 3.889 0.257 0.0028** 61.3 Example 2 4.979 0.713 0.0045** 106.5 Example 3 5.841 0.771 0.0020** 142.3 Example 4 6.359 0.727 0.001** 163.7 Comparative Example 1 2.660 0.036 0.2178 10.3 Comparative Example 2 2.303 0.064 0.5666 -4.5 Comparative Example 3 3.851 0.274 0.0034** 59.7 Comparative Example 4 2.908 0.195 0.0708* 20.6 Comparative Example 5 3.271 0.418 0.0433* 35.7 Comparative Example 6 3.849 0.336 0.005** 59.6 Comparative Example 7 2.937 0.158 0.0521* 21.8

[0071] (Note: *, **, *** represent p<0.05, p<0.01, p<0.001, respectively.)

[0072] It can be seen from Table 4 that the use of silanized hyaluronic acid ester alone (i.e., comparative example 1) has a very weak promoting effect on the expression of ZO-1 protein, and the use of sodium hyaluronate alone (i.e., comparative example 2) has no promoting effect on the expression of ZO-1 protein. The capernaum fruit composite liquid obtained by method 2 (i.e., comparative example 4) has a weaker promoting effect on the expression of ZO-1 protein, which is not as strong as the promoting effect obtained by method 1 (i.e., comparative example 3). Examples 1 to 4 have a promoting effect on the expression of ZO-1 protein of 61.3%, 106.5%, 142.3%, and 163.7%, respectively, indicating that the compounding of silanized hyaluronic acid ester and the capernaum fruit composite liquid obtained by method 1 has a significant synergistic effect.

[0073] Table 5 Summary of TGM-5 protein content test results

[0074] Sample name Average RFU SD P-value Upregulation rate (%, vs SLS group) NC Group 32.140 2.623 / / SLS Group 7.704 0.660 / / Example 1 13.769 1.581 0.0036** 78.7 Example 2 19.779 2.179 0.0008*** 156.7 Example 3 21.312 2.453 0.0008*** 176.6 Example 4 22.180 2.937 0.0011** 187.9 Comparative Example 1 9.811 0.185 0.006** 27.3 Comparative Example 2 8.300 0.716 0.3489 7.7 Comparative Example 3 11.456 0.074 0.0006*** 48.7 Comparative Example 4 9.270 0.031 0.0148* 20.3 Comparative Example 5 10.292 0.094 0.0025** 33.6 Comparative Example 6 11.122 1.176 0.0118* 44.4 Comparative Example 7 9.352 0.021 0.0124* 21.4

[0075] (Note: *, **, *** represent p<0.05, p<0.01, p<0.001, respectively.)

[0076] It can be seen from Table 5 that the use of silanized hyaluronic acid ester alone and sodium hyaluronate alone (i.e., Comparative Examples 1 and 2) has a weaker effect on promoting the expression of TGM-5 protein, especially in Comparative Example 2. The caper fruit composite liquid obtained by method 2 (i.e., Comparative Example 4) has a weaker effect on promoting the expression of TGM-5 protein, which is not as strong as the one obtained by method 1 (i.e., Comparative Example 3). Examples 1 to 4 have a significant synergistic effect on the expression of TGM-5 protein, which is 78.7%, 156.7%, 176.6%, and 187.9%, respectively, indicating that the compounding of silanized hyaluronic acid ester and the caper fruit composite liquid obtained by method 1 has a significant synergistic effect.

[0077] Test Example 2: Evaluation of human moisturizing and repairing effects

[0078] The skin barrier repairing essence cream of Example 5 was tested for its moisturizing and repairing effects on human skin. A total of 33 subjects (5 males and 28 females) completed the test, aged 20 to 40 years old, meeting the voluntary inclusion and exclusion criteria of the subjects.

[0079] Usage: After cleansing in the morning and evening, apply an appropriate amount of the essence cream of Example 5 evenly on the face, and massage gently until absorbed. Use twice a day, once in the morning and once in the evening, for 28 consecutive days.

[0080] Detection time: before using the sample (D0), 14 days after using the sample (D14), and 28 days after using the sample (D28).

[0081] Test method:

[0082] 1. The facial image analyzer VISIA-CR is used for red zone image analysis to obtain the quantitative index red zone analysis a* value.

[0083] 2. The skin moisture content tester Corneometer is used to detect the relative moisture content of the skin surface.

[0084] 3. Skin water loss tester Aqua Flux is used to detect the transepidermal water loss (TWEL) value of the skin and evaluate the skin barrier function.

[0085] Experimental parameters:

[0086] 1. Skin moisture content: The larger the skin moisture content value, the higher the skin moisture content.

[0087] 2. Transepidermal water loss (TWEL) value: The lower the TWEL value, the better the skin barrier. The calculation formula is as follows

[0089]

[0090] 3. Facial red zone a* value: The larger the a* value of the red zone analysis, the more severe the skin redness.

[0091] SPSS Statistics 25 was used for statistical analysis, with two-tailed test and test level α = 0.05.

[0092] The measurement data were analyzed by the normal distribution test results and the difference analysis method was selected: if the measured values ​​were normally distributed, the t-test method was used for statistical analysis; if they were non-normally distributed, the rank sum test method was used for statistical analysis.

[0093] The test results are shown in Tables 6 to 8.

[0094] Table 6 Descriptive statistics of red zone analysis a* (n=33)

[0095]

[0096] Statistical analysis results: “-”: no statistically significant difference (P≥0.05); “*”: statistically significant difference (0.01≤P<0.05); “**”: statistically significant difference (0.001≤P<0.01); “***”: statistically significant difference (P<0.001).

[0097] Table 7 Descriptive statistics of skin moisture content (n=33)

[0098]

[0099]

[0100] Statistical analysis results: “-”: no statistically significant difference (P≥0.05); “*”: statistically significant difference (0.01≤P<0.05); “**”: statistically significant difference (0.001≤P<0.01); “***”: statistically significant difference (P<0.001).

[0101] Table 8 Descriptive statistics of transepidermal water loss TEWL values ​​(n=33)

[0102]

[0103] Statistical analysis results: “-”: no statistically significant difference (P≥0.05); “*”: statistically significant difference (0.01≤P<0.05); “**”: statistically significant difference (0.001≤P<0.01); “***”: statistically significant difference (P<0.001).

[0104] As shown in Tables 6 to 8, in the 4-week test period, Example 5 significantly improved the facial red area (the facial red area changes of subjects No. 2 and No. 13 before and after using the samples are shown in the figure below). Figure 1-2 As shown in the figure, the transepidermal water loss TEWL of the skin is significantly reduced, and the water content of the skin is significantly increased. Therefore, the essence cream containing the skin barrier repairing composition has a good effect of improving facial moisturizing and redness repairing.

[0105] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for repairing skin barrier, characterized in that: The invention comprises the following components in parts by weight: 0.01-0.1 parts of silanized hyaluronic acid ester with an average molecular weight of 700-800 kDa and 0.5-10.0 parts of caper fruit composite liquid.

2. A skin barrier repairing composition as claimed in claim 1, characterized in that: The preparation method of the caper fruit composite liquid is specifically as follows: adding water to caper fruit powder and stirring and extracting for 1-3 times at 60-90° C., and combining the filtrate; then adding a composite enzyme consisting of α-amylase, acid protease and neutral protease for enzymolysis to obtain a crude extract, and filtering; then adding polyamide resin and activated carbon for adsorption, and then adding glycerol and mixing evenly to obtain a caper fruit composite liquid, which is 3.5-4.0 times the mass of the caper fruit powder.

3. The skin barrier repairing composition according to claim 2, characterized in that: The invention comprises the following components in parts by weight: 0.03-0.08 parts of silanized hyaluronic acid ester with an average molecular weight of 700-800 kDa and 1.0-8.0 parts of caper fruit composite liquid.

4. The skin barrier repairing composition according to claim 2 or 3, characterized in that: The silanized hyaluronic acid ester and the caper fruit composite liquid were dissolved in water to make up to 100 parts.

5. Use of the skin barrier repairing composition according to claim 2 or 4 in preparing cosmetics for repairing the skin barrier and improving skin dryness and redness.

6. A cosmetic for repairing skin barrier, characterized in that: The cosmetic comprises at least the skin barrier repairing composition according to claim 4.

7. A cosmetic for repairing skin barrier according to claim 6, characterized in that: The amount of the skin barrier repairing composition according to claim 4 added is 0.1-10% based on the total mass of the cosmetic.

Images