Organic peptide plant nutrient and preparation method thereof

Abstract

The invention discloses an organic peptide plant nutrient and a preparation method thereof, and belongs to the field of agricultural product preparation. The organic peptide plant nutrient comprises CEP peptide, an adsorbent, phosphate, an amino acid fertilizer, a nitrogen fertilizer and a potash fertilizer, the amino acid fertilizer properly replaces the nitrogen fertilizer, the content of nitrate nitrogen in soil can be remarkably increased, nitrogen absorption of plants can be improved by increasing the content of the nitrate nitrogen in the soil, meanwhile, the added CEP peptide also promotes the nitrate nitrogen absorption capacity of root systems, and the nitrogen absorption capacity of the plants is improved. Meanwhile, due to the added modified bentonite, the adsorption of heavy metal ions can be improved, the soil environment is improved, the dosage of nutritional agents is reduced, the nitrogen fertilizer utilization rate is increased, the purposes of increasing the yield and increasing the efficiency in agricultural production are achieved, and a new method is provided for developing novel plant nutritional agents.

Description

Technical Field

[0001] The invention belongs to the field of agricultural product preparation, and specifically relates to an organic peptide plant nutrient and a preparation method thereof. Background Art

[0002] Plant nutrients are a class of substances that have similar physiological and biological effects to plant hormones. The effects of plant nutrients are generally obvious and stable, and are less affected by environmental conditions. Therefore, they are quickly promoted and applied, and are easily accepted by users. Plant nutrients can be applied to almost all higher and lower plants in the planting industry. They control the growth and development of plants by regulating the photosynthesis, respiration, absorption and operation of substances, signal transduction, stomatal opening and closing, osmotic regulation, transpiration and other physiological processes of plants, improve the interaction between plants and the environment, enhance the stress resistance of crops, increase crop yields, improve the quality of agricultural products, and make the expression of crop agronomic traits develop in the direction required by people.

[0003] Adsorbing or chelating organic peptides with other substances to play a synergistic or new role is a novel research direction. The patent application with publication number CN 101481266 A discloses a plant nutrient and preparation method and application, which is made by chelating small molecule collagen peptides with medium and / or trace elements. Small molecule collagen peptides themselves have the function of promoting cell proliferation and maintaining cell division. After element chelation, they can also provide pure natural nutrition to fruits, vegetables, grains, cotton and other crops, increase crop yields, and improve the quality of agricultural products.

[0004] How to develop a new type of plant nutrient that is conducive to direct absorption by plants, supplements plants with high-quality nutrients, balances plant growth, improves immunity, resists diseases and pests, reduces the use of pesticides, can provide plant nutrition for a long time, and is completely non-toxic and harmless, is a new idea in the agricultural products industry. Summary of the invention

[0005] The object of the present invention is to provide an organic peptide plant nutrient and a preparation method thereof, so as to promote plant growth and reduce the amount of nutrient used.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] An organic peptide plant nutrient comprises, by weight, 1 to 3 parts of CEP peptide, 10 to 15 parts of adsorbent, 20 to 30 parts of phosphate, 40 to 60 parts of amino acid fertilizer, 60 to 80 parts of nitrogen fertilizer and 10 to 20 parts of potash fertilizer.

[0008] Furthermore, the adsorbent is one of modified bentonite and carbon nanotubes.

[0009] Furthermore, the modified bentonite is prepared by the following steps:

[0010] The first step is to add 20% hydrochloric acid solution to the mixture of bentonite and deionized water, and stir in a water bath at 75-80°C for 3-3.5 hours, then wash with distilled water until it is nearly neutral, filter, dry, grind, and sieve to obtain acidified bentonite;

[0011] In the second step, the acidified bentonite is mixed with γ-mercaptopropyltrimethoxysilane and anhydrous ethanol in proportion, and the mixture is shaken and reacted at room temperature for 3 to 3.5 hours, and then washed with anhydrous ethanol and distilled water until neutral, filtered, dried, ground and sieved to obtain the modified bentonite.

[0012] Furthermore, in the first step, by weight, 1 to 2 parts of bentonite, 10 to 15 parts of deionized water, and 4 to 6 parts of 20% hydrochloric acid solution are used.

[0013] Furthermore, in the second step, by weight, 10 to 12 parts of acidified bentonite, 1 to 2 parts of γ-mercaptopropyltrimethoxysilane, and 20 to 25 parts of anhydrous ethanol are used.

[0014] Furthermore, the phosphate is one of potassium hydrogen phosphate, ammonium phosphate and disodium hydrogen phosphate.

[0015] Furthermore, the amino acid fertilizer has an organic matter content of 26.2-28.1%, a total nitrogen content of 5.36-6.48%, a total phosphorus content of 0.21-0.38%, a total potassium content of 0.33-0.50%, and a pH value of 5.1-5.24.

[0016] Furthermore, the nitrogen fertilizer is one of urea and ammonium nitrate.

[0017] Furthermore, the potash fertilizer is one of potassium chloride and potassium sulfate.

[0018] A method for preparing an organic peptide plant nutrient comprises the following steps:

[0019] The CEP peptide, adsorbent, phosphate, amino acid fertilizer, nitrogen fertilizer and potassium fertilizer are mixed to obtain an organic peptide plant nutrient.

[0020] Beneficial effects of the present invention:

[0021] (1) The CEP peptide used in the present invention plays a major role when there is a lack of nitrogen or uneven availability of nitrogen for plant growth in the soil. Under nitrogen deficiency stress, the roots produce CEP peptides, which are sensed by receptors in the stems as "nitrogen starvation signals", which in turn activate and induce the expression of nitrate transporters in the roots. By exogenously adding an appropriate concentration of CEP peptides, the expression of nitrate transport genes can be upregulated, thereby promoting the root system's ability to absorb nitrate nitrogen.

[0022] (2) The adsorbent used in the present invention has a large specific surface area and a strong adsorption capacity, and has the ability to adsorb metal ions. On the one hand, it reduces the heavy metal content, and on the other hand, it provides the required metal elements for plant growth. Furthermore, bentonite can adsorb polypeptide molecules due to its large interlayer domain. Through modification, the adsorption of metal ions can be improved. On the other hand, bentonite also plays a promoting role in improving the soil environment and increasing the utilization rate of nutrients.

[0023] (3) The amino acid fertilizer used in the present invention, under the condition of the same nitrogen application amount, adds a certain proportion of amino acid fertilizer to the soil, which has a higher content of ammonium nitrogen and nitrate nitrogen than that of chemical fertilizer nitrogen. It works synergistically with CEP peptide to reduce the amount of nutrients used, improve nitrogen utilization, and promote plant growth and development.

[0024] (4) The phosphate used in the present invention not only provides phosphorus nutrition for plants, but also has a passivating effect on heavy metals. The released phosphate radicals react with heavy metal ions to generate phosphate minerals with lower solubility. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Example 1

[0027] This embodiment provides an organic peptide plant nutrient, which is prepared by the following steps:

[0028] In the first step, 4 parts of 20% hydrochloric acid solution are added to a mixture of 1 part of bentonite and 10 parts of deionized water, and the mixture is stirred in a water bath at 80°C for 3 hours, and then washed with distilled water until it is nearly neutral, filtered, dried, ground, and sieved to obtain acidified bentonite;

[0029] The second step is to mix 2 parts of acidified bentonite with 1 part of γ-mercaptopropyltrimethoxysilane and 20 parts of anhydrous ethanol in a certain proportion, and shake and react at room temperature for 3 hours, then wash with anhydrous ethanol and distilled water until neutral, filter, dry, grind and sieve to obtain modified bentonite;

[0030] The third step is to mix 1 part of CEP peptide, 10 parts of modified bentonite, 20 parts of potassium hydrogen phosphate, 40 parts of amino acid fertilizer, 60 parts of urea, and 10 parts of potassium chloride to obtain organic peptide plant nutrients.

[0031] Example 2

[0032] The difference between this example and Example 1 is that "1 part of CEP peptide" is changed to "3 parts of CEP peptide".

[0033] The remaining raw materials and preparation process remain the same as in Example 1.

[0034] Example 3

[0035] The difference between this embodiment and Example 1 is that "40 parts of amino acid fertilizer" is changed to "60 parts of amino acid fertilizer".

[0036] The remaining raw materials and preparation process remain the same as in Example 1.

[0037] Example 4

[0038] Compared with Example 1, this embodiment differs in that the first and second steps are removed and the adsorbent is replaced with carbon nanotubes. The specific implementation steps are:

[0039] Mix 1 part of CEP peptide, 10 parts of carbon nanotubes, 20 parts of potassium hydrogen phosphate, 40 parts of amino acid fertilizer, 60 parts of urea, and 10 parts of potassium chloride to obtain an organic peptide plant nutrient.

[0040] The remaining raw materials and preparation process remain the same as in Example 1.

[0041] Example 5

[0042] The present embodiment is different from the embodiment 1 in that "potassium hydrogen phosphate" is replaced by "disodium hydrogen phosphate".

[0043] The remaining raw materials and preparation process remain the same as in Example 1.

[0044] Example 6

[0045] The present embodiment is different from the embodiment 1 in that "urea" is replaced by "ammonium nitrate".

[0046] The remaining raw materials and preparation process remain the same as in Example 1.

[0047] Example 7

[0048] The difference between this embodiment and embodiment 1 is that "potassium chloride" is changed to "potassium sulfate".

[0049] The remaining raw materials and preparation process remain the same as in Example 1.

[0050] Comparative Example 1

[0051] Compared with Example 1, this comparative example is different in that the second step is removed. The specific implementation steps are:

[0052] In the first step, 4 parts of 20% hydrochloric acid solution are added to a mixture of 1 part of bentonite and 10 parts of deionized water, and stirred in a water bath at 80°C for 3 hours, and then washed with distilled water until nearly neutral, filtered, dried, ground, and sieved to obtain acidified bentonite.

[0053] In the second step, mix 1 part of CEP peptide, 10 parts of modified bentonite, 20 parts of potassium hydrogen phosphate, 40 parts of amino acid fertilizer, 60 parts of urea, and 10 parts of potassium chloride to obtain organic peptide plant nutrients.

[0054] The remaining raw materials and preparation process remain the same as in Example 1.

[0055] Comparative Example 2

[0056] The difference between this comparative example and Example 1 is that no CEP peptide is added.

[0057] The remaining raw materials and preparation process remain the same as in Example 1.

[0058] Comparative Example 3

[0059] The difference between this comparative example and Example 1 is that no adsorbent is added.

[0060] The remaining raw materials and preparation process remain the same as in Example 1.

[0061] Performance tests were performed on Examples 1 to 7 and Comparative Examples 1 to 3:

[0062] The samples prepared in Examples 1 to 7 and Comparative Examples 1 to 3 were diluted 1000 times with water respectively. Starting from the 30th day after the tomato plants were planted, the organic peptide plant nutrient was applied once every 7 to 9 days for 7 consecutive times. The nitrate nitrogen content in the soil was determined by a flow analyzer on the 75th and 120th days. The heavy metal content in the soil was determined on the 120th day according to DB61 / T 902.2-2013 "Determination of Heavy Metal Elements in Plant Extracts". Tomato samples were selected for experimental determination 120 days after planting.

[0063] Nitrogen fertilizer utilization rate (%) = (total nitrogen absorption of plants in the nitrogen application area - total nitrogen absorption of plants in the non-nitrogen application area) / nitrogen application amount × 100

[0064] The results are shown in Table 1:

[0065] Table 1

[0066]

[0067] It can be seen from Table 1 that the nitrate nitrogen content in the soil was the highest 75 days after the organic peptide plant nutrient was applied in Example 1. This is because the amino acid fertilizer applied appropriately replaces the nitrogen fertilizer and can significantly increase the nitrate nitrogen content in the soil during the tomato growth period. Increasing the nitrate nitrogen content in the soil can increase the absorption of nitrogen by tomatoes. At the same time, the added CEP peptide promotes the root system's absorption capacity of nitrate nitrogen, which corresponds to the lowest soil nitrate nitrogen content at 120 days. Compared with Example 1, Examples 2-3 increase the dosage of CEP and amino acid fertilizer respectively, but the results show that the utilization rate of nitrate nitrogen decreases instead, indicating that too high a concentration will inhibit plant growth. Therefore, reasonable control of the application ratio of nutrients and CEP peptides can increase the total nitrogen absorption of tomatoes, promote the absorption and utilization of nitrogen by tomato plants, and achieve the purpose of increasing production and efficiency.

[0068] By comparing the results of Example 4, Comparative Example 1 and Comparative Example 3 with other examples and comparative examples, after the adsorbent was replaced with carbon nanotubes, the adsorbent was treated differently and no adsorbent was added, the results showed that the heavy metal content of plants increased, indicating that the modified bentonite has a stronger adsorption performance for heavy metals, because the modified bentonite has more thiol groups, which is equivalent to adding many adsorption sites, which can be connected with heavy metal ions in the form of coordination bonds, and this binding mode is more stable, which illustrates the superiority of the modified bentonite in fixing heavy metals; Compared with Example 1, Comparative Example 3 increased the nitrate nitrogen content in the soil after 120 days without adding modified bentonite, because the modified bentonite has an adsorption effect on CEP peptide, so it is more conducive to being absorbed by plants, thereby further playing a role; Compared with Example 1, Comparative Example 2 did not add CEP peptide, resulting in a corresponding weakening of the plant's absorption of nitrate nitrogen, showing a trend of reduced production.

[0069] In summary, the organic peptide plant nutrient and its preparation method provided by the present invention promote the absorption and utilization of nitrogen by tomato plants by increasing the content of nitrate nitrogen in the soil and improving the root system's ability to absorb nitrate nitrogen; at the same time, the fixation of heavy metals reduces their harmful effects on plants, promotes the growth and development of plants, and provides a new idea for increasing production and efficiency in agricultural production.

[0070] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0071] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An organic peptide plant nutrient, characterized in that: Calculated by weight, it includes 1 to 3 parts of CEP peptide, 10 to 15 parts of adsorbent, 20 to 30 parts of phosphate, 40 to 60 parts of amino acid fertilizer, 60 to 80 parts of nitrogen fertilizer, and 10 to 20 parts of potash fertilizer.

2. An organic peptide plant nutrient according to claim 1, characterized in that: The adsorbent is one of modified bentonite and carbon nanotube.

3. An organic peptide plant nutrient according to claim 2, characterized in that: The modified bentonite is prepared by the following steps: The first step is to add 20% hydrochloric acid solution to the mixture of bentonite and deionized water, and stir in a water bath at 75-80°C for 3-3.5 hours, then wash with distilled water until it is nearly neutral, filter, dry, grind, and sieve to obtain acidified bentonite; In the second step, the acidified bentonite is mixed with γ-mercaptopropyltrimethoxysilane and anhydrous ethanol in proportion, and the mixture is shaken and reacted at room temperature for 3 to 3.5 hours, and then washed with anhydrous ethanol and distilled water until neutral, filtered, dried, ground and sieved to obtain the modified bentonite.

4. An organic peptide plant nutrient according to claim 3, characterized in that: In the first step, by weight, 1 to 2 parts of bentonite, 10 to 15 parts of deionized water, and 4 to 6 parts of 20% hydrochloric acid solution are used.

5. The organic peptide plant nutrient according to claim 3, characterized in that: In the second step, by weight, 10-12 parts of acidified bentonite, 1-2 parts of γ-mercaptopropyltrimethoxysilane and 20-25 parts of anhydrous ethanol are used.

6. The organic peptide plant nutrient according to claim 1, characterized in that: The phosphate is one of potassium hydrogen phosphate, ammonium phosphate and disodium hydrogen phosphate.

7. The organic peptide plant nutrient according to claim 1, characterized in that: The nitrogen fertilizer is one of urea and ammonium nitrate.

8. The organic peptide plant nutrient according to claim 1, characterized in that: The potash fertilizer is one of potassium chloride and potassium sulfate.

9. A method for preparing the organic peptide plant nutrient according to any one of claims 1 to 8, characterized in that: The following steps are involved: The CEP peptide, adsorbent, phosphate, amino acid fertilizer, nitrogen fertilizer and potassium fertilizer are mixed to obtain an organic peptide plant nutrient.

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