Composite strain and application thereof in manufacturing of mushroom dreg organic fertilizer
By using the complex strains composed of Pseudomonas aeruginosa and Serratia syrup, the problem of poor treatment of edible fungi was solved, and efficient organic fertilizer for bacterial residues was prepared, which increased the nutrient content of the fertilizer. and crop growth effects, reducing environmental pollution.
Patent Information
- Application Number
- CN202411229220.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-03
- Publication Date
- 2024-12-10
AI Technical Summary
Poor treatment of edible fungal residues leads to environmental pollution and waste of resources. Traditional organic fertilizers have low nutrient content and poor stability, making it difficult to effectively utilize the fertilizer potential of bacterial residues.
Using a complex strain composed of Pseudomonas aeruginosaX-1 and Serratia serratiamarcescens, the bacterial residues are fermented with bacterial residues after activation and mixing to prepare efficient bacterial residue organic fertilizer, and the fermentation conditions are controlled to improve the fertilizer. Contents of organic matter, nitrogen, phosphorus and potassium.
It has achieved efficient preparation of bacterial residue organic fertilizer, shortened the fermentation cycle, improved the organic matter content and nutrient abundance of the fertilizer, significantly improved the growth effect of crops, and reduced environmental pollution.
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Figure CN119101617A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of microbial fermentation, and in particular to a composite strain and application thereof in the preparation of fungus residue organic fertilizer. Background Art
[0002] The edible fungus industry is known as white agriculture. The industry provides the protein needed for human life through edible fungi or medicinal fungi, and has broad development prospects. However, a large amount of fungus residue will be produced in the process of edible fungus cultivation. The annual consumption of edible fungi in my country is huge, and a large amount of fungus residue is produced. However, due to various factors, these edible fungus residues have not been well treated. If these huge amounts of fungus residues are not effectively treated and utilized, they will not only harm the ecological environment, but also cause serious waste of resources. At present, there is no very reasonable method for treating fungus residues.
[0003] Regarding edible fungi residue, it also has great utilization value in itself. For example, edible fungi residue contains rich bacterial protein, multiple metabolites and underutilized nutrients, and has a high organic matter content, which is a good compost raw material. Therefore, in the treatment method of edible fungi residue, the edible fungi residue can be recycled and used as fertilizer for crops. Generally, the residue is placed naturally for fermentation, that is, the edible fungi residue that has not been processed is directly spread on the field, which can generally roughly play a certain degree of fertilizer effect, improve soil, improve soil permeability, improve physical and chemical properties, enhance crop quality, and increase yield. However, after the untreated residue is placed for a long time or randomly discarded in an untreated place, it will easily mold and deteriorate, produce an unpleasant odor, cause inconvenience in application, cause soil and water pollution, and thus endanger the ecological environment and people's health. Therefore, the edible fungi residue needs to be processed before it can safely and greenly play its fertilizer role.
[0004] Bio-organic fertilizer refers to a type of fertilizer that combines the effects of microorganisms and organic fertilizers, which are mainly derived from animal and plant residues (such as livestock and poultry manure, crop straw, etc.) and are harmlessly treated and decomposed organic materials. The functions of bio-organic fertilizers mainly include improving soil, improving soil water and fertilizer retention capacity, promoting plant growth, improving fertilizer utilization, soil repair and degradation of pesticides. Traditional organic fertilizers have relatively low nutrient content, slow fertilizer effect, sometimes poor stability, incomplete fermentation, low fertilizer maturity, and are prone to burning seedlings. They are high in toxic and harmful substances such as heavy metals, and are easy to spread soil-borne diseases and insect pests. The odor in the fertilizer will also affect its application. For the production of fertilizer from edible fungus residue, the use of bio-organic fertilizer is a green and feasible solution.
[0005] my country is a large agricultural country with a large demand for fertilizers. Edible mushroom residue can be used to make biological organic fertilizers, which can increase crop yields and improve quality. Compared with traditional fertilizers, although it can greatly increase crop yields, it also causes considerable damage to the land. The preparation of biological organic fertilizers from edible mushroom residues is a green and environmentally friendly treatment method that protects the environment and caters to sustainable development policies. Summary of the invention
[0006] The invention aims to provide a composite bacterial strain and application thereof in the production of fungus residue organic fertilizer.
[0007] To achieve the above object, the present invention adopts the following technical solutions:
[0008] The invention provides a composite strain, wherein the composite strain is prepared by mixing two strains; one of the strains is Pseudomonas aeruginosa X-1, which was deposited in China Center for Type Culture Collection on July 8, 2024, with a deposit number of CCTCC NO: M20241488; the other strain is Serratia marcescens X-2, which was deposited in China Center for Type Culture Collection on July 8, 2024, with a deposit number of CCTCC NO: M20241489.
[0009] As another aspect of the present invention, the present invention provides the use of the composite strain in the production of fungus residue organic fertilizer.
[0010] Preferably, the Pseudomonas aeruginosa and Serratia marcescens are activated before catalyzing the fermentation of bacterial residues, and are inoculated into a sterilized LB medium at an inoculation rate of 1% for culture.
[0011] Preferably, the conditions for culturing and activating Pseudomonas aeruginosa and Serratia marcescens in LB medium are: liquid volume fraction of 10%, culture temperature of 37° C., shaker speed of 200 rpm, and culture time of 12 h.
[0012] Preferably, the composite strain is prepared by mixing Pseudomonas aeruginosa and Serratia marcescens in a ratio of 1:2.5.
[0013] Preferably, the composite strain is diluted with sterile water in proportion after activation, and then evenly mixed with edible fungus residues under different placement conditions. After mixing, the mixture is placed under a range of conditions, and after fermentation and composting, the preparation of the residue organic fertilizer is completed; the placement conditions are stacking placement or barrel-shaped container placement.
[0014] Preferably, the temperature of the edible fungus residue is 21-24°C before fermentation begins, the temperature of the residue begins to rise on the third day of fermentation, and rises to 45-50°C on the fifth day, and physical turning, aeration and spraying with clean water are performed, and the physical turning from the inside to the outside is performed 3-5 times to prevent the temperature of the edible fungus residue from being too high so that the fermentation can continue; thereafter, physical turning and spraying with clean water are performed every two days, and the fermentation and decomposition are continued until 10-15 days, when the temperature of the edible fungus residue drops back to room temperature, the decomposition is completed, and the residue organic fertilizer is obtained; wherein the water content of the residue organic fertilizer drops to below 50%, it is not sticky, does not flow with sewage and has no stench.
[0015] After adopting the above technical solution, the present invention has the following beneficial effects:
[0016] 1. The Pseudomonas aeruginosa and Serratia marcescens of the present invention are functional strains selected from bacterial residue fermentation fertilizers and play a special role in bacterial residue fermentation.
[0017] 2. The method for applying Pseudomonas aeruginosa and Serratia marcescens to fertilizer fermentation using edible fungi residues is easy to operate and highly efficient, and the fermentation and decomposition cycle is relatively short (about 10-15 days). The finally obtained residue organic fertilizer has high contents of organic matter, N, P and K, and has good industrial application prospects in the technical field of preparing organic fertilizers by microbial fermentation. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 The nutritional composition diagram of the fungus residue organic fertilizer sample obtained in Example 1 of the present invention;
[0019] Figure 2 The nutritional composition diagram of the fungus residue organic fertilizer sample obtained in Example 2 of the present invention;
[0020] Figure 3 The nutritional composition diagram of the fungus residue organic fertilizer sample obtained in Example 3 of the present invention;
[0021] Figure 4 The first day growth condition diagram of the chicken feather vegetable in the fertilizer efficiency test of the present invention;
[0022] Figure 5 The figure is a growth condition diagram of the 10th day of the chicken feather vegetable in the fertilizer efficiency test of the present invention;
[0023] Figure 6 It is a growth condition diagram of the 15th day of the chicken feather vegetable in the fertilizer efficiency test of the present invention. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0025] like Figures 1 to 6 shown.
[0026] Example 1
[0027] (1) Strain activation: The corresponding strain samples were weighed in proportion to the weight and volume of the fungus residue to be fermented, and added to the LB medium sterilized with high temperature and high pressure at an inoculum of 1% for culture activation. The culture activation conditions were: the volume fraction of the liquid was 10%, the culture temperature was 37°C, the shaking speed was 200 rpm, and the culture time was 12 h. The activated bacterial solution was mixed in a ratio of 1:2.5 to obtain a composite bacterial solution.
[0028] (2) Fermentation of fungus residue: The activated bacterial liquid in step (1) is diluted in sufficient amount according to the weight volume of the fungus residue to be fermented, and the pile of fungus residue is selected for fermentation. The weight of the fungus residue of the pile is 130kg. After the diluted bacterial liquid and the fungus residue are mixed evenly, the humidity of the fungus residue is adjusted to 60%-65%, and then the pile is placed for fermentation. The temperature in the fungus residue pile is recorded regularly every day. The pile temperature is 21-24°C before the start of fermentation. The fungus residue pile begins to heat up on the third day of fermentation, and heats up to 45-50°C on the fifth day. Physical digging, aeration and spraying of clean water are carried out, and physical digging from the inside to the outside is carried out 3-5 times, so that the pile temperature is not too high and can continue to ferment. After that, physical digging and spraying of clean water are started every two days, and fermentation and decomposition are continued until 10-15d, when the pile temperature drops back to room temperature, decomposition is completed, and fungus residue organic fertilizer is obtained.
[0029] (3) Fertility test of bacterial fertilizer: The moisture content of the fully decomposed bacterial residue organic fertilizer drops below 50%, it is not sticky, does not flow with sewage and has no bad smell. Take the fully decomposed bacterial residue organic fertilizer sample in the middle of the pile for fertility test, and test its N, P, K and organic matter content (dry organic matter). According to the measurement, the organic matter content of the fermented and decomposed bacterial residue organic fertilizer reaches 59% (dry organic matter), containing N1.32%, P1.05%, and K9.54% (by mass fraction), which far exceeds the organic fertilizer industry standard NY525-2002, which stipulates that the organic matter content is ≥30% and the total nutrient content N+P+K is ≥4.0%.
[0030] Example 2
[0031] (1) Strain activation: The corresponding strain samples were weighed in proportion to the weight and volume of the fungus residue to be fermented, and added to the LB medium sterilized with high temperature and high pressure at an inoculum of 1% for culture activation. The culture activation conditions were: the volume fraction of the liquid was 10%, the culture temperature was 37°C, the shaking speed was 200 rpm, and the culture time was 12 h. The activated bacterial solution was mixed in a ratio of 1:2.5 to obtain a composite bacterial solution.
[0032] (2) Fermentation of fungus residue: The activated bacterial liquid in step (1) is diluted in sufficient amount according to the weight volume of the fungus residue to be fermented, and the weight of the fungus residue in the pile is 130kg. After the diluted bacterial liquid and the fungus residue are mixed evenly, the humidity of the fungus residue is adjusted to 60%-65%, and the pile is placed for fermentation. The temperature in the fungus residue pile is recorded regularly every day. The pile temperature is 21-24°C before fermentation begins. The fungus residue pile begins to heat up on the third day of fermentation, and heats up to 45-50°C on the fifth day. Physical digging, aeration and spraying of clean water are carried out, and physical digging from the inside to the outside is carried out 3-5 times so that the pile temperature is not too high and fermentation can continue. After that, physical digging and spraying of clean water are started every two days, and fermentation and decomposition are continued until 10-15d, when the pile temperature drops back to room temperature, decomposition is completed, and fungus residue organic fertilizer is obtained.
[0033] (3) Fertility test of bacterial fertilizer: The moisture content of the fully decomposed bacterial residue organic fertilizer drops below 50%, it is not sticky, does not flow with sewage and has no bad smell. Take the sample of the fully decomposed bacterial residue organic fertilizer outside the pile for fertility test, and test its N, P, K and organic matter content (dry organic matter). According to the measurement, the organic matter content of the fermented and decomposed bacterial residue organic fertilizer reaches 60% (dry organic matter), containing N 0.69%, P 0.92%, and K 8.80% (by mass fraction), which far exceeds the organic fertilizer industry standard NY525-2002 that the organic matter content is ≥30% and the total nutrient content N+P+K is ≥4.0%.
[0034] Example 3
[0035] (1) Strain activation: The corresponding strain samples were weighed in proportion to the weight and volume of the fungus residue to be fermented, and added to the LB medium sterilized with high temperature and high pressure at an inoculum of 1% for culture activation. The culture activation conditions were: the volume fraction of the liquid was 10%, the culture temperature was 37°C, the shaking speed was 200 rpm, and the culture time was 12 h. The activated bacterial solution was mixed in a ratio of 1:2.5 to obtain a composite bacterial solution.
[0036] (2) Fermentation of fungus residue: The activated bacterial liquid in step (1) is diluted in sufficient amount according to the weight volume of the fungus residue to be fermented, and barreled fungus residue is selected for fermentation. The weight of the barreled fungus residue is 10 kg. After the diluted bacterial liquid and the fungus residue are mixed evenly, the humidity of the fungus residue is adjusted to 60%-65%, and the fungus residue is placed in a barrel container for fermentation. The temperature of the fungus residue in the barrel is recorded regularly every day. Before the fermentation starts, the temperature of the fungus residue in the barrel is 21-24°C. On the third day of fermentation, the temperature of the fungus residue in the barrel begins to rise, and on the fifth day, the temperature rises to 45-50°C. Physical turning, aeration and spraying with clean water are carried out, and physical turning from the inside to the outside is carried out 3-5 times so that the temperature of the fungus residue in the barrel is not too high and can continue to ferment. After that, physical turning and spraying with clean water every two days are started, and fermentation and decomposition are continued until 10-15 days, when the temperature of the fungus residue in the barrel drops back to room temperature, decomposition is completed, and fungus residue organic fertilizer is obtained.
[0037] (3) Fertility test of bacterial fertilizer: The moisture content of the fully decomposed bacterial residue organic fertilizer drops below 50%, and it is not sticky, does not flow with sewage, and has no bad smell. Take the bacterial residue organic fertilizer sample in the middle of the fully decomposed bacterial residue fertilizer in the barrel for fertility test, and test its N, P, K, and organic matter content (dry organic matter). According to the measurement, the organic matter content of the fermented bacterial residue organic fertilizer reaches 60% (dry organic matter), containing N 0.70%, P 1.23%, and K 10.49% (by mass fraction), which far exceeds the organic fertilizer industry standard NY525-2002, which stipulates that the organic matter content is ≥30% and the total nutrient content N+P+K is ≥4.0%.
[0038] The nutritional components of the fungus residue organic fertilizer sample obtained in Example 1 are as follows: Figure 1 The nutritional components of the fungus residue organic fertilizer sample obtained in Example 2 are as shown in Figure 2 The nutritional components of the fungus residue organic fertilizer sample obtained in Example 3 are as shown in Figure 4 shown.
[0039] Fertilizer efficiency test:
[0040] Experiment on vegetable planting with fungus residue organic fertilizer - planting of Chinese cabbage
[0041] Test scope: small planting pots.
[0042] Test time period: 15 days.
[0043] A control experiment was conducted on the planting of radish in a planting pot. The planting pot was divided into two areas, and the same amount of planting soil was placed and the same amount of radish seeds were sown. Different treatments: a small portion of the mushroom residue organic fertilizer prepared in Example 1 was taken to the left area of the planting pot, and the right area of the planting pot was not treated, and the two sides were separated by a partition.
[0044] After the treatment is completed, place the planting pots in a natural environment and observe the growth of the vegetable seedlings regularly.
[0045] Growth status on the first day Figure 4 As shown;
[0046] Growth status on the tenth day Figure 5 As shown;
[0047] Growth status on the fifteenth day Figure 6 As shown;
[0048] The test results show that the growth of the Chinese pear seedlings in the control area (left side of the planting pot) and the test area (right side of the planting pot) is very different. The Chinese pear seedlings in the test area where the fungus residue organic fertilizer prepared by Example 1 of the present invention is applied grow vigorously, far exceeding the growth of the Chinese pear seedlings in the control area where the fungus residue organic fertilizer of the present invention is not applied. The application effect of the fungus residue organic fertilizer of the present invention is significant.
[0049] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.
Claims
1. A composite strain, characterized in that: The composite strain is prepared by mixing two strains; one of the strains is Pseudomonas aeruginosa X-1, which was deposited in the China Center for Type Culture Collection on July 8, 2024, with a deposit number of CCTCC NO: M20241488; the other strain is Serratia marcescens X-2, which was deposited in the China Center for Type Culture Collection on July 8, 2024, with a deposit number of CCTCC NO: M20241489.
2. Application of the composite strain as claimed in claim 1 in the production of fungus residue organic fertilizer.
3. The application of the composite strain as claimed in claim 2 in the production of fungus residue organic fertilizer is characterized in that: The Pseudomonas aeruginosa and Serratia marcescens are activated before catalyzing the fermentation of bacterial residues, and are inoculated into the sterilized LB culture medium at an inoculation rate of 1% for culture.
4. The application of the composite strain as claimed in claim 3 in the production of fungus residue organic fertilizer is characterized in that: The conditions for culturing and activating Pseudomonas aeruginosa and Serratia marcescens in LB medium are as follows: the volume fraction of the liquid is 10%, the culture temperature is 37° C., the shaking speed is 200 rpm, and the culture time is 12 hours.
5. The application of the composite strain as claimed in claim 3 in the production of fungus residue organic fertilizer is characterized in that: The composite strain is prepared by mixing Pseudomonas aeruginosa and Serratia marcescens in a ratio of 1:2.
5.
6. The application of the composite strain as claimed in claim 3 in the production of fungus residue organic fertilizer is characterized in that: The composite strain is diluted with sterile water in proportion after activation, and then evenly mixed with edible fungus residues under different placement conditions. After mixing, the mixture is placed under a range of conditions, and after fermentation and composting, the preparation of the fungus residue organic fertilizer is completed; the placement conditions are stacking placement or barrel-shaped container placement.
7. The application of the composite strain as claimed in claim 6 in the production of fungus residue organic fertilizer is characterized in that: The temperature of the edible fungus residue is 21-24°C before fermentation begins. The temperature of the residue begins to rise on the third day of fermentation and rises to 45-50°C on the fifth day. Physical turning, aeration and spraying with clean water are performed from the inside to the outside for 3-5 times to prevent the temperature of the edible fungus residue from being too high so that the fermentation can continue. After that, physical turning and spraying with clean water are performed every two days to continue fermentation and decomposition until 10-15 days, when the temperature of the edible fungus residue drops back to room temperature, decomposition is completed, and a residue organic fertilizer is obtained. The moisture content of the residue organic fertilizer drops below 50%, and it is not sticky, does not flow with sewage and has no bad smell.