A SNP locus combination for constructing a coconut SNP fingerprint map and its application

Abstract

The embodiment of the present application relates to an SNP locus combination for constructing a coconut SNP fingerprint map and its application, belonging to the technical field of molecular biology. The embodiment of the present application aims to solve the technical problem that the previous fingerprints are usually screened for molecular markers based on SSR markers or other experimental means, resulting in poor repeatability. The SNP loci of the embodiment of the present application include 260 SNP loci located on chromosomes Chr01 to Chr16. The SNP locus combination provided by the present application can be used for constructing a coconut SNP fingerprint map and rapid identification of coconut varieties, and the present application provides brand-new identity information exclusive to different coconut varieties, which can effectively identify the authenticity of individuals and trace the origin of individuals, and has good application prospects in aspects such as coconut germplasm resource classification and identification, and molecular-assisted breeding.

Description

Technical Field

[0001] The embodiments of the present application relate to the field of molecular biology technology, and in particular to a SNP site combination and application for constructing a coconut SNP fingerprint map. Background Art

[0002] Coconut (Cocos nucifera L.) is an important tropical oil crop, landscape plant and fruit tree, known as the "tree of life". Coconut is widely planted in about 93 countries and regions in the world, with a total global planting area of ​​about 180 million mu, and plays a vital role in the economic life of people in tropical regions. Coconut has a planting history of more than 2,000 years in my country, and is planted in Hainan, Yunnan, Guangdong, Guangxi and other places. Coconut is widely distributed in Asia, Africa, America and Pacific island countries, and there are rich local specialty varieties, including African high species, Hainan high species, Sri Lanka high species, red dwarf, yellow dwarf, brown dwarf, perfume and other varieties. The numerous varieties and complex genetic backgrounds have brought great challenges to the identification of coconut varieties.

[0003] DNA fingerprinting technology is a modern method based on DNA molecular markers that can be used for the classification and identification of germplasm resources. Constructing DNA fingerprints of plant varieties can overcome the limitations of identifying varieties based solely on morphological characteristics, which is very important for the accurate identification of germplasm resources and gene discovery. DNA fingerprinting technology is widely used in the study of variety resource diversity and variety identification, but there are few reports on coconuts. In addition, most of the existing variety identification standards are based on SSR, RAPD, and RFLP markers, and it is rare to construct fingerprints based on SNP molecular markers to achieve coconut variety identification. Summary of the invention

[0004] In view of this, the embodiment of the present application provides a SNP site combination and application for constructing a coconut SNP fingerprint map. 327 coconut samples are taken as research objects, and the fingerprint calculation software GaSnp based on genetic algorithm is used to accurately obtain the SNP site combination with high polymorphism and distinguish all samples. 260 core SNP site information is obtained by calculation, and the unique fingerprint map belonging to each coconut sample is constructed using these SNP site information. The constructed fingerprint map can be used for classification and identification of coconut germplasm resources and breeding, etc., which can effectively overcome the defect that the previous fingerprints are usually based on SSR markers or other experimental means for molecular marker screening, and have poor repeatability.

[0005] In a first aspect, an embodiment of the present application provides a SNP site combination for constructing a coconut SNP fingerprint map, wherein the SNP site combination includes 260 SNP sites located on chromosomes Chr01 to Chr16.

[0006] In some embodiments that may include the above embodiments, the SNP loci include:

[0007] Locus 1, which is located at 11190781 bp on chromosome 1, and the mutation type is T / G;

[0008] Locus 2, which is located at 12235818 bp on chromosome 1, and the mutation type is G / A;

[0009] Locus 3, which is located at 17980352 bp on chromosome 1, and the mutation type is A / G;

[0010] Locus 4, which is located at 110680094 bp on chromosome 1, and the mutation type is A / G;

[0011] Locus 5, which is located at 144832968 bp on chromosome 1, and the mutation type is C / G;

[0012] Locus 6, which is located at 146014481 bp on chromosome 1, and the mutation type is T / A;

[0013] Locus 7, which is located at 146793777 bp on chromosome 1, and the mutation type is A / G;

[0014] Locus 8, which is located at 154417159 bp on chromosome 1, and the mutation type is C / T;

[0015] Locus 9, which is located at 157934494 bp on chromosome 1, and the mutation type is A / G;

[0016] Locus 10, which is located at 159488786 bp on chromosome 1, and the mutation type is A / G;

[0017] Locus 11, which is located at 1177659160 bp on chromosome 1, and the mutation type is A / G;

[0018] Locus 12, which is located at 1205295810 bp on chromosome 1, and the mutation type is C / T;

[0019] Locus 13, which is located at 1209649951 bp on chromosome 1, and the mutation type is G / A;

[0020] Locus 14, which is located at 1210539345 bp on chromosome 1, and the mutation type is T / C;

[0021] Locus 15, which is located at 1213678628 bp on chromosome 1, and the mutation type is T / C;

[0022] Locus 16, which is located at 25511596 bp on chromosome 2, and the mutation type is A / T;

[0023] Locus 17, which is located at 242777399 bp on chromosome 2, with a variant type of T / C; Locus 18, which is located at 2104373536 bp on chromosome 2, with a variant type of C / T; Locus 19, which is located at 2134120172 bp on chromosome 2, with a variant type of T / C; Locus 20, which is located at 2139741917 bp on chromosome 2, with a variant type of T / C; Locus 21, which is located at 2140969969 bp on chromosome 2, with a variant type of A / G; Locus 22, which is located at 2141004076 bp on chromosome 2, with a variant type of A / G; Locus 23, which is located at 2144312732 bp on chromosome 2, with a variant type of C / G; Locus 24, which is located at 2155951920 bp on chromosome 2, with a variant type of C / T; Locus 25, which is located at 2168814238 bp on chromosome 2, with a variant type of G / A; Locus 26, which is located at 2171030095 bp on chromosome 2, with a variant type of T / A; Locus 27, which is located at 2173296480 bp on chromosome 2, with a variant type of A / G; Locus 28, which is located at 2175131148 bp on chromosome 2, with a variant type of C / G; Locus 29, which is located at 2175229826 bp on chromosome 2, with a variant type of T / C; Locus 30, which is located at 2177772806 bp on chromosome 2, with a variant type of A / C; Locus 31, which is located at 2184028384 bp on chromosome 2, with a variant type of G / A; Locus 32, which is located at 2184558347 bp on chromosome 2, with a variant type of T / C; Locus 33, which is located at 2185183473 bp on chromosome 2, with a variant type of T / A; Locus 34, which is located at 2186374217 bp on chromosome 2, with a variant type of C / T; Locus 35, which is located at 37077949 bp on chromosome 3, with a variant type of C / A; Locus 36, which is located at 39467662 bp on chromosome 3, with a variant type of A / G; Locus 37, which is located at 323482912 bp on chromosome 3, with a variant type of G / A; Locus 38, which is located at 333226223 bp on chromosome 3, with a variant type of G / A; Locus 39, which is located at 333957544 bp on chromosome 3, with a variant type of A / G; Locus 40, which is located at 334035592 bp on chromosome 3, with a variant type of A / G; Locus 41, which is located at 344675912 bp on chromosome 3, with a variant type of C / T;Locus 42, which is located at 349598945 bp on chromosome 3, with a variant type of A / G; Locus 43, which is located at 349967597 bp on chromosome 3, with a variant type of A / G; Locus 44, which is located at 350712941 bp on chromosome 3, with a variant type of G / T; Locus 45, which is located at 355447906 bp on chromosome 3, with a variant type of A / G; Locus 46, which is located at 356509055 bp on chromosome 3, with a variant type of C / T; Locus 47, which is located at 357193563 bp on chromosome 3, with a variant type of T / C; Locus 48, which is located at 363014229 bp on chromosome 3, with a variant type of G / A; Locus 49, which is located at 364932466 bp on chromosome 3, with a variant type of G / A; Locus 50, which is located at 368304699 bp on chromosome 3, with a variant type of C / T; Locus 51, which is located at 3160699507 bp on chromosome 3, with a variant type of G / A; Locus 52, which is located at 3161671764 bp on chromosome 3, with a variant type of G / A; Locus 53, which is located at 3168627960 bp on chromosome 3, with a variant type of T / C; Locus 54, which is located at 3170406066 bp on chromosome 3, with a variant type of C / T; Locus 55, which is located at 3171148457 bp on chromosome 3, with a variant type of T / C; Locus 56, which is located at 3171572870 bp on chromosome 3, with a variant type of T / A; Locus 57, which is located at 3173329295 bp on chromosome 3, with a variant type of T / C; Locus 58, which is located at 3181396863 bp on chromosome 3, with a variant type of C / T; Locus 59, which is located at 3185748145 bp on chromosome 3, with a variant type of T / A; Locus 60, which is located at 43488085 bp on chromosome 4, with a variant type of A / G; Locus 61, which is located at 49185469 bp on chromosome 4, with a variant type of T / C; Locus 62, which is located at 420370567 bp on chromosome 4, with a variant type of T / C; Locus 63, which is located at 422158504 bp on chromosome 4, with a variant type of G / A; Locus 64, which is located at 493029245 bp on chromosome 4, with a variant type of A / G; Locus 65, which is located at 4133737637 bp on chromosome 4, with a variant type of T / A; Locus 66, which is located at 4136800305 bp on chromosome 4, with a variant type of T / C; Locus 67, which is located at 4140123972 bp on chromosome 4, with a variant type of A / C;Locus 68, which is located at position 4142148415bp on chromosome 4, with the mutation type being T / G; Locus 69, which is located at position 4150103302bp on chromosome 4, with the mutation type being T / C; Locus 70, which is located at position 4150752420bp on chromosome 4, with the mutation type being A / G; Locus 71, which is located at position 4151876715bp on chromosome 4, with the mutation type being G / A; Locus 72, which is located at position 4153027020bp on chromosome 4, with the mutation type being G / A; Locus 73, which is located at position 4154635908bp on chromosome 4, with the mutation type being C / T; Locus 74, which is located at position 4157572576bp on chromosome 4, with the mutation type being G / T; Locus 75, which is located at position 4162608762bp on chromosome 4, with the mutation type being G / C; Locus 76, which is located at position 4163952706bp on chromosome 4, with the mutation type being T / C; Locus 77, which is located at position 4164312912bp on chromosome 4, with the mutation type being G / A; Locus 78, which is located at position 4167462144bp on chromosome 4, with the mutation type being T / C; Locus 79, which is located at position 4174584167bp on chromosome 4, with the mutation type being T / C; Locus 80, which is located at position 4175575716bp on chromosome 4, with the mutation type being T / A; Locus 81, which is located at position 4179391103bp on chromosome 4, with the mutation type being A / G; Locus 82, which is located at position 5101002bp on chromosome 5, with the mutation type being C / A;

[0024] Locus 83, which is located at position 54050477bp on chromosome 5, with the mutation type being T / C;

[0025] Locus 84, which is located at position 56210415bp on chromosome 5, with the mutation type being C / T;

[0026] Locus 85, which is located at position 57353166bp on chromosome 5, with the mutation type being T / C;

[0027] Locus 86, which is located at position 57907806bp on chromosome 5, with the mutation type being T / G;

[0028] Locus 87, which is located at 58122046 bp on chromosome 5, with a variant type of C / T; Locus 88, which is located at 512139653 bp on chromosome 5, with a variant type of A / G; Locus 89, which is located at 512139824 bp on chromosome 5, with a variant type of T / C; Locus 90, which is located at 513420754 bp on chromosome 5, with a variant type of T / C; Locus 91, which is located at 516715793 bp on chromosome 5, with a variant type of C / T; Locus 92, which is located at 521548705 bp on chromosome 5, with a variant type of A / G; Locus 93, which is located at 522923012 bp on chromosome 5, with a variant type of T / C; Locus 94, which is located at 524040590 bp on chromosome 5, with a variant type of T / C; Locus 95, which is located at 527470277 bp on chromosome 5, with a variant type of T / C; Locus 96, which is located at 530565269 bp on chromosome 5, with a variant type of A / C; Locus 97, which is located at 531991099 bp on chromosome 5, with a variant type of A / T; Locus 98, which is located at 535018979 bp on chromosome 5, with a variant type of G / T; Locus 99, which is located at 536993723 bp on chromosome 5, with a variant type of G / T; Locus 100, which is located at 537746327 bp on chromosome 5, with a variant type of G / A; Locus 101, which is located at 543138430 bp on chromosome 5, with a variant type of T / C; Locus 102, which is located at 543944445 bp on chromosome 5, with a variant type of C / T; Locus 103, which is located at 550429114 bp on chromosome 5, with a variant type of T / C; Locus 104, which is located at 561180240 bp on chromosome 5, with a variant type of A / G; Locus 105, which is located at 565131503 bp on chromosome 5, with a variant type of G / A; Locus 106, which is located at 570663061 bp on chromosome 5, with a variant type of A / T; Locus 107, which is located at 5144968891 bp on chromosome 5, with a variant type of C / T; Locus 108, which is located at 5144969368 bp on chromosome 5, with a variant type of T / A; Locus 109, which is located at 5155396989 bp on chromosome 5, with a variant type of A / C; Locus 110, which is located at 5161684131 bp on chromosome 5, with a variant type of C / A; Locus 111, which is located at 5161685301 bp on chromosome 5, with a variant type of T / C;Locus 112, which is located at position 5164032398 bp on chromosome 5, with a variant type of A / T; Locus 113, which is located at position 5164856997 bp on chromosome 5, with a variant type of A / G; Locus 114, which is located at position 6778804 bp on chromosome 6, with a variant type of T / C; Locus 115, which is located at position 62344276 bp on chromosome 6, with a variant type of G / A; Locus 116, which is located at position 63607324 bp on chromosome 6, with a variant type of G / A; Locus 117, which is located at position 69812751 bp on chromosome 6, with a variant type of C / G; Locus 118, which is located at position 615808467 bp on chromosome 6, with a variant type of C / T; Locus 119, which is located at position 617634309 bp on chromosome 6, with a variant type of G / A; Locus 120, which is located at position 622461159 bp on chromosome 6, with a variant type of A / T; Locus 121, which is located at position 626460149 bp on chromosome 6, with a variant type of G / A; Locus 122, which is located at position 627819611 bp on chromosome 6, with a variant type of T / C; Locus 123, which is located at position 636016335 bp on chromosome 6, with a variant type of G / T; Locus 124, which is located at position 6142497880 bp on chromosome 6, with a variant type of C / A; Locus 125, which is located at position 6142498639 bp on chromosome 6, with a variant type of G / T; Locus 126, which is located at position 6143663591 bp on chromosome 6, with a variant type of A / G; Locus 127, which is located at position 6145585715 bp on chromosome 6, with a variant type of A / C; Locus 128, which is located at position 6149622668 bp on chromosome 6, with a variant type of G / A; Locus 129, which is located at position 6157761880 bp on chromosome 6, with a variant type of C / G; Locus 130, which is located at position 6170724270 bp on chromosome 6, with a variant type of C / G; Locus 131, which is located at position 6170763361 bp on chromosome 6, with a variant type of A / G; Locus 132, which is located at position 71710306 bp on chromosome 7, with a variant type of T / C; Locus 133, which is located at position 73264083 bp on chromosome 7, with a variant type of C / G; Locus 134, which is located at position 77548057 bp on chromosome 7, with a variant type of A / C; Locus 135, which is located at position 77750449 bp on chromosome 7, with a variant type of T / A; Locus 136, which is located at position 712649262 bp on chromosome 7, with a variant type of T / A;Locus 137, which is located at position 712909310 bp on chromosome 7, with the variant type being A / G; Locus 138, which is located at position 712989315 bp on chromosome 7, with the variant type being C / G; Locus 139, which is located at position 713199095 bp on chromosome 7, with the variant type being T / C; Locus 140, which is located at position 714493374 bp on chromosome 7, with the variant type being C / G; Locus 141, which is located at position 718532969 bp on chromosome 7, with the variant type being C / G; Locus 142, which is located at position 723461275 bp on chromosome 7, with the variant type being C / T; Locus 143, which is located at position 729251772 bp on chromosome 7, with the variant type being C / T; Locus 144, which is located at position 737148816 bp on chromosome 7, with the variant type being C / T; Locus 145, which is located at position 747094940 bp on chromosome 7, with the variant type being T / C; Locus 146, which is located at position 751336679 bp on chromosome 7, with the variant type being C / A; Locus 147, which is located at position 7162732167 bp on chromosome 7, with the variant type being A / G; Locus 148, which is located at position 81371158 bp on chromosome 8, with the variant type being A / G; Locus 149, which is located at position 86789650 bp on chromosome 8, with the variant type being G / A; Locus 150, which is located at position 88437771 bp on chromosome 8, with the variant type being T / C; Locus 151, which is located at position 814588094 bp on chromosome 8, with the variant type being G / A; Locus 152, which is located at position 815736361 bp on chromosome 8, with the variant type being A / G;

[0029] Locus 153, which is located at position 825810325 bp on chromosome 8, with the variant type being G / C;

[0030] Locus 154, which is located at position 826325818 bp on chromosome 8, with the variant type being T / G;

[0031] Locus 155, which is located at position 837475933 bp on chromosome 8, with the variant type being T / C;

[0032] Locus 156, which is located at position 874738903 bp on chromosome 8, with the variant type being A / G; Locus 157, which is located at position 8138002896 bp on chromosome 8, with the variant type being C / T; Locus 158, which is located at position 8142021696 bp on chromosome 8, with the variant type being C / A; Locus 159, which is located at position 8145208971 bp on chromosome 8, with the variant type being C / T; Locus 160, which is located at position 8146286654 bp on chromosome 8, with the variant type being A / G; Locus 161, which is located at position 8150404881 bp on chromosome 8, with the variant type being T / A; Locus 162, which is located at position 8151594514 bp on chromosome 8, with the variant type being T / C; Locus 163, which is located at position 91438383 bp on chromosome 9, with the variant type being C / T;

[0033] Locus 164, which is located at position 911449710 bp on chromosome 9, with the variant type being T / C;

[0034] Locus 165, which is located at position 913479358 bp on chromosome 9, with the variant type being G / C;

[0035] Locus 166, which is located at position 913489579 bp on chromosome 9, with the variant type being C / G;

[0036] Locus 167, which is located at 941147490 bp on chromosome 9, with the mutation type being A / C; Locus 168, which is located at 9141925303 bp on chromosome 9, with the mutation type being T / C; Locus 169, which is located at 9142775138 bp on chromosome 9, with the mutation type being T / C; Locus 170, which is located at 9147110013 bp on chromosome 9, with the mutation type being C / T; Locus 171, which is located at 9147846861 bp on chromosome 9, with the mutation type being G / A; Locus 172, which is located at 9151353465 bp on chromosome 9, with the mutation type being C / T; Locus 173, which is located at 9153453729 bp on chromosome 9, with the mutation type being T / C; Locus 174, which is located at 9153698062 bp on chromosome 9, with the mutation type being T / A; Locus 175, which is located at 9154824375 bp on chromosome 9, with the mutation type being C / G; Locus 176, which is located at 9155548198 bp on chromosome 9, with the mutation type being A / G; Locus 177, which is located at 9155548252 bp on chromosome 9, with the mutation type being G / A; Locus 178, which is located at 9155576669 bp on chromosome 9, with the mutation type being C / G; Locus 179, which is located at 102302094 bp on chromosome 10, with the mutation type being G / C; Locus 180, which is located at 109432633 bp on chromosome 10, with the mutation type being T / C; Locus 181, which is located at 1017416902 bp on chromosome 10, with the mutation type being A / T; Locus 182, which is located at 10121954875 bp on chromosome 10, with the mutation type being G / A; Locus 183, which is located at 10130315170 bp on chromosome 10, with the mutation type being A / T; Locus 184, which is located at 10132041600 bp on chromosome 10, with the mutation type being G / T; Locus 185, which is located at 10137888718 bp on chromosome 10, with the mutation type being C / T; Locus 186, which is located at 10142022816 bp on chromosome 10, with the mutation type being A / G; Locus 187, which is located at 10143214786 bp on chromosome 10, with the mutation type being G / A; Locus 188, which is located at 10143714885 bp on chromosome 10, with the mutation type being A / G; Locus 189, which is located at 10144007893 bp on chromosome 10, with the mutation type being C / T; Locus 190, which is located at 1155339362 bp on chromosome 11, with the mutation type being G / A;Locus 191, which is located at 1190715627 bp on chromosome 11, with the variant type of A / G; Locus 192, which is located at 1194597680 bp on chromosome 11, with the variant type of T / G; Locus 193, which is located at 1194839888 bp on chromosome 11, with the variant type of A / T; Locus 194, which is located at 1195818114 bp on chromosome 11, with the variant type of T / C; Locus 195, which is located at 11105015249 bp on chromosome 11, with the variant type of G / A; Locus 196, which is located at 11118023633 bp on chromosome 11, with the variant type of C / A; Locus 197, which is located at 11128125475 bp on chromosome 11, with the variant type of A / G; Locus 198, which is located at 11133467339 bp on chromosome 11, with the variant type of G / A; Locus 199, which is located at 11138493485 bp on chromosome 11, with the variant type of C / T; Locus 200, which is located at 123684982 bp on chromosome 12, with the variant type of G / A; Locus 201, which is located at 125995960 bp on chromosome 12, with the variant type of G / A; Locus 202, which is located at 126835408 bp on chromosome 12, with the variant type of T / C; Locus 203, which is located at 1213550741 bp on chromosome 12, with the variant type of T / C; Locus 204, which is located at 1213899205 bp on chromosome 12, with the variant type of A / C; Locus 205, which is located at 1214447869 bp on chromosome 12, with the variant type of G / A; Locus 206, which is located at 12107960617 bp on chromosome 12, with the variant type of T / G; Locus 207, which is located at 12119478235 bp on chromosome 12, with the variant type of T / C; Locus 208, which is located at 12120018613 bp on chromosome 12, with the variant type of C / T; Locus 209, which is located at 12124086849 bp on chromosome 12, with the variant type of G / T; Locus 210, which is located at 12124453241 bp on chromosome 12, with the variant type of G / A; Locus 211, which is located at 12128376164 bp on chromosome 12, with the variant type of T / G; Locus 212, which is located at 12128559402 bp on chromosome 12, with the variant type of G / A; Locus 213, which is located at 12128953055 bp on chromosome 12, with the variant type of C / T; Locus 214, which is located at 12130582077 bp on chromosome 12, with the variant type of A / G;Locus 215, which is located at position 12132475469 bp on chromosome 12, with a variant type of A / G; Locus 216, which is located at position 12134372645 bp on chromosome 12, with a variant type of A / C; Locus 217, which is located at position 12138491493 bp on chromosome 12, with a variant type of C / T; Locus 218, which is located at position 12140124081 bp on chromosome 12, with a variant type of C / G; Locus 219, which is located at position 12140239381 bp on chromosome 12, with a variant type of G / T; Locus 220, which is located at position 1327030687 bp on chromosome 13, with a variant type of A / G; Locus 221, which is located at position 1334684042 bp on chromosome 13, with a variant type of C / T; Locus 222, which is located at position 1345894210 bp on chromosome 13, with a variant type of C / T; Locus 223, which is located at position 1353577453 bp on chromosome 13, with a variant type of A / G; Locus 224, which is located at position 1353812335 bp on chromosome 13, with a variant type of A / G; Locus 225, which is located at position 1360489824 bp on chromosome 13, with a variant type of T / G; Locus 226, which is located at position 1367836217 bp on chromosome 13, with a variant type of C / T; Locus 227, which is located at position 1373891012 bp on chromosome 13, with a variant type of T / C; Locus 228, which is located at position 1376533867 bp on chromosome 13, with a variant type of A / G; Locus 229, which is located at position 1383098051 bp on chromosome 13, with a variant type of T / C; Locus 230, which is located at position 1388748630 bp on chromosome 13, with a variant type of G / A; Locus 231, which is located at position 1389057411 bp on chromosome 13, with a variant type of C / T; Locus 232, which is located at position 1390232542 bp on chromosome 13, with a variant type of A / G; Locus 233, which is located at position 1390395203 bp on chromosome 13, with a variant type of A / G; Locus 234, which is located at position 1465570441 bp on chromosome 14, with a variant type of G / A; Locus 235, which is located at position 1469425337 bp on chromosome 14, with a variant type of A / G; Locus 236, which is located at position 1475905772 bp on chromosome 14, with a variant type of G / A; Locus 237, which is located at position 1478843002 bp on chromosome 14, with a variant type of T / C; Locus 238, which is located at position 1481865290 bp on chromosome 14, with a variant type of C / T;Locus 239, which is located at 1481945414 bp on chromosome 14, with the mutation type being A / T; Locus 240, which is located at 1484299928 bp on chromosome 14, with the mutation type being C / T; Locus 241, which is located at 1485726361 bp on chromosome 14, with the mutation type being G / A; Locus 242, which is located at 1488144162 bp on chromosome 14, with the mutation type being G / A;

[0037] Locus 243, which is located at 1489143409 bp on chromosome 14, with the mutation type being G / A;

[0038] Locus 244, which is located at 153183570 bp on chromosome 15, with the mutation type being C / T;

[0039] Locus 245, which is located at 154250994 bp on chromosome 15, with the mutation type being G / A;

[0040] Locus 246, which is located at 157960137 bp on chromosome 15, with the mutation type being A / G;

[0041] Locus 247, which is located at 1512731347 bp on chromosome 15, with the mutation type being A / G;

[0042] Locus 248, which is located at 1515374666 bp on chromosome 15, with the mutation type being C / T;

[0043] Locus 249, which is located at 1520140486 bp on chromosome 15, with the mutation type being T / G;

[0044] Locus 250, which is located at 1526989810 bp on chromosome 15, with the mutation type being A / T;

[0045] Locus 251, which is located at 1585734438 bp on chromosome 15, with the mutation type being C / T;

[0046] Locus 252, which is located at 1632401183 bp on chromosome 16, with the mutation type being G / A;

[0047] Locus 253, which is located at 1649669601 bp on chromosome 16, with the mutation type being T / C;

[0048] Locus 254, which is located at 1656467304 bp on chromosome 16, with the mutation type being A / G;

[0049] Locus 255, which is located at 1662417000 bp on chromosome 16, with the mutation type being G / T;

[0050] Locus 256, which is located at 1665186178 bp on chromosome 16, with the mutation type being A / G;

[0051] Locus 257, which is located at 1667185586 bp on chromosome 16, with the mutation type being A / G;

[0052] Locus 258, which is located at 1668159645 bp on chromosome 16, with the mutation type being T / C;

[0053] Locus 259, which is located at 1670703024 bp on chromosome 16, with the mutation type being G / A;

[0054] Locus 260, which is located at 1674142244 bp on chromosome 16, with the mutation type being T / G.

[0055] The second aspect of the embodiments of the present application further provides a coconut DNA fingerprint map, which is constructed by using the above SNP locus combination.

[0056] The third aspect of the embodiments of the present application further provides the application of the above SNP locus combination in constructing a coconut DNA fingerprint map.

[0057] The fourth aspect of the embodiments of the present application further provides the application of the above SNP locus combination in the classification and identification of coconut germplasm resources.

[0058] The fifth aspect of the embodiments of the present application further provides a method for identifying coconut germplasm by using the above SNP locus combination, including the following steps:

[0059] (1) Extract coconut sample DNA;

[0060] (2) Perform genome sequencing of a single variety on the coconut sample DNA;

[0061] (3) Rely on the sequencing results to perform genotyping after alignment with the dwarf coconut reference genome (website: http: / / arecaceae-gdb.com / # / download, genome name: cocos_nucifera_dwarf.genome.fa), and identify the SNP locus information of each variety;

[0062] (4) Determine the similarity between the test sample and the known fingerprint sample according to the genotype combination of the known fingerprint SNP loci, so as to evaluate the variety and origin information of the coconut.

[0063] In some embodiments which may include the above embodiments, the coconut sample is a fresh coconut leaf.

[0064] Compared with the prior art, the embodiments of the present application have the following beneficial effects:

[0065] (1) Based on the SNP locus combination obtained from coconut re-sequencing, the present application has SNP loci evenly distributed in chromosomes and high polymorphism, which can be used to construct a coconut SNP fingerprint map and rapidly identify coconut varieties.

[0066] (2) The present application provides brand-new identity information exclusive to 327 coconut germplasms, which can effectively identify the authenticity of individuals and trace the origin of individuals.

[0067] (3) Using the SNP locus combination, the present application effectively constructs a coconut SNP fingerprint map and establishes a genotype ID card for coconuts. Only by detecting the corresponding genomic sequences of the samples to be tested and comparing them with the fingerprint map library can the origin classification of the samples to be tested be identified, the genetic relationship between varieties be judged, and the genetic distance between varieties be measured; the coconut SNP fingerprint map obtained in the present application has a high accuracy in coconut variety identification. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for description in the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0069] Figure 1 a is a phylogenetic tree constructed with the original 7,650,000 SNPs, Figure 1 b is a phylogenetic tree constructed with 260 fingerprint SNP loci, where blue represents the tall coconut group and purple represents the dwarf coconut group, and the population structure and clustering results are basically consistent. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0070] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art without creative efforts based on the embodiments of the present application belong to the scope of protection of the present application.

[0071] Unless otherwise specified, the experimental methods used in the embodiments of the present application are all conventional methods.

[0072] In the following embodiments, unless otherwise specified, all raw materials can be obtained through commercial purchase or conventional methods.

[0073] Example 1 - Obtaining SNP Locus Combinations and Constructing Fingerprint Maps of Coconut Varieties

[0074] All SNP loci were developed based on re - sequencing data. The original SNP loci were filtered to remove SNP loci with a missing number greater than 1% and homozygous genotypes 0 / 0 and 1 / 1 with a count less than 2. The correlation between each SNP locus was calculated, and adjacent SNP loci with a correlation R 2 > 0.95 were used as alternative loci for subsequent genetic algorithms.

[0075] Using the GaSnp software, the genotype format was converted and the SNP loci were randomly combined to calculate a set of filtered SNP locus combinations that can distinguish all varieties. Finally, 260 core SNP loci were selected to construct the fingerprint maps of coconut varieties. The names, chromosomes, positions, mutation types, and allele frequencies of each SNP locus are shown in Table 1, and the fingerprint maps of each variety are shown in Table 2.

[0076] In this application, the 327 coconut materials in Table 2 were identified using the SNP loci obtained in Table 1, and the corresponding fingerprint sequences of each variety were obtained. As can be seen from the table, the fingerprint sequences of each variety are different, indicating that the 327 materials can be effectively distinguished, achieving accurate identification of different coconut varieties.

[0077] Table 1 SNP Locus Combinations for Constructing Coconut SNP Fingerprint Maps

[0078]

[0079]

[0080]

[0081]

[0082]

[0083]

[0084] Table 2 Coconut Varieties and Their Fingerprint Sequences

[0085]

[0086]

[0087]

[0088]

[0089]

[0090]

[0091]

[0092]

[0093]

[0094]

[0095]

[0096]

[0097]

[0098]

[0099]

[0100]

[0101]

[0102]

[0103]

[0104]

[0105]

[0106]

[0107]

[0108]

[0109] In the fingerprint coding table of different coconut samples in Table 2, "0" in the fingerprint coding refers to the genotype 0 / 0; it indicates that the locus in a certain sample of the coconut sample is homozygous, that is, the same as the locus in the reference genome; "2" in the fingerprint coding refers to the genotype 0 / 1, indicating that the locus in the sample is heterozygous, that is, partially the same as the locus in the reference genome; "1" in the fingerprint coding refers to the genotype 1 / 1; it indicates that the locus in the sample has a variation, that is, completely different from the locus in the reference genome.

[0110] Example 2 - Identification of Coconut Germplasm

[0111] The method for identifying coconut germplasm using the above SNP locus combination includes the following steps:

[0112] (1) Using fresh coconut leaves as coconut samples, extract the DNA of coconut samples by the CTAB method:

[0113] ① Take about 2 g of fresh leaves, put them into a pre-cooled mortar, add about 0.08 g of PVP, quickly grind them into powder in liquid nitrogen, transfer them to a pre-cooled 50 mL centrifuge tube, add 20 mL of pre-heated 2% CTAB lysis buffer, slowly invert up and down to mix well, and then incubate in a water bath at 65 °C for 90 min. During the water bath, invert and mix once every about 15 min;

[0114] ② Take out the centrifuge tube and cool it to room temperature, add an equal volume of chloroform / isoamyl alcohol (24:1) to mix well, balance and let it stand for 10 min, put it into a centrifuge, and centrifuge at 12000 r / min for 10 min. Absorb the supernatant and transfer it to a new 50 mL centrifuge tube, repeat the previous step; absorb the supernatant and transfer it to a new 50 mL centrifuge tube, add pre-cooled absolute ethanol, pick out the floating DNA, finally collect the DNA into a 1.5 mL centrifuge tube, wash it with 75% absolute ethanol, air-dry the DNA precipitate, add TE buffer, dissolve it and then add RNase A (20 mg / mL), incubate in a water bath at 37 °C for 30 min, and take it out and store it at 4 °C for later use;

[0115] (2) Use the high-throughput sequencing method to detect the SNP loci in the coconut sample DNA:

[0116] ① Use the PCR amplification primer pair combination for amplifying the SNP locus combination to perform PCR amplification and electrophoresis detection on the coconut sample DNA; prepare the PCR amplification system (2 μl DNA, 1 μl primer, 17 μl mix solution) in a PCR plate, set the PCR amplification program; prepare a 2% agarose gel, and take 4 μl of the PCR amplification product for electrophoresis detection; ② Send the PCR product containing the target band to a sequencing company for sequencing to obtain the corresponding sequence information;

[0117] (3) Align the sequencing sequence with the reference genome, and use the GATK software for genotyping to identify the SNP loci, and rely on the similarity degree of the variation information of the SNP loci with 327 germplasm banks to identify the tested coconut variety and source information.

[0118] Example 3 - Verification of the Accuracy of Coconut SNP Fingerprint

[0119] By analyzing the genetic diversity and genetic relationship among coconut varieties with high repeatability and similarity, it is found that the cluster analysis using molecular marker method is completely consistent with the traditional classification, indicating that the obtained coconut fingerprint map can be effectively used for the classification and identification of coconut germplasm resources, including:

[0120] (1) Using the SNP locus combination of the present application above, evaluate the genetic variation and structure of the obtained coconut population, and it is found that there is relatively rich genetic variation in the obtained coconut sample population;

[0121] (2) Analyze the genetic diversity and genetic relationship of the obtained coconut variety resources, and it is found that the clustering result is consistent with the traditional morphological classification result, and the genetic similarity coefficient is high;

[0122] (3) The phylogenetic tree constructed using the coconut SNP locus combination of the present application is the same as the phylogenetic tree constructed using other loci, indicating that the 327 coconut SNP fingerprint loci obtained are sufficient to restore the population structure of the coconut population, and this fingerprint map can effectively identify different coconut samples with high accuracy, as Figure 1 shown.

[0123] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. Application of SNP marker combination in constructing coconut DNA fingerprint, the SNP marker combination includes 260 SNP markers located on chromosomes Chr01 to Chr16, the SNP markers include: Marker No. 1, which is located on chromosome 1, bp 11,190,781, and the variant type is T / G; Marker No. 2, which is located on chromosome 1 at bp 12235818, has a variant type of G / A; Marker No. 3, which is located on chromosome 1, bp 17980352, and the variant type is A / G; Marker No. 4, which is located on chromosome 1, bp 110680094, and the variant type is A / G; Marker No. 5, which is located on chromosome 1, bp 144832968, has a variation type of C / G; Marker No. 6, which is located on chromosome 1, bp 146014481, and the variant type is T / A; Marker No. 7, which is located on chromosome 1, bp 146793777, and the variant type is A / G; Marker No. 8, which is located on chromosome 1, bp 154417159, has a variation type of C / T; Marker No. 9, which is located on chromosome 1, bp 157934494, and the variant type is A / G; Marker No. 10 is located on chromosome 1, bp 159488786, and the variant type is A / G; Marker No. 11 is located on chromosome 1 at bp 1177659160, and the variant type is A / G; Marker No. 12, which is located on chromosome 1, bp 1205295810, and the variant type is C / T; Marker No. 13 is located on chromosome 1, bp 1209649951, and the variant type is G / A; Marker No. 14 is located on chromosome 1, bp 1210539345, and the variant type is T / C; Marker No. 15, which is located on chromosome 1, bp 1213678628, has a variation type of T / C; Marker No. 16, which is located on chromosome 2, bp 25511596, and the variant type is A / T; Marker 17 is located on chromosome 2, bp 242777399, and the variant type is T / C; Marker 18, which is located on chromosome 2, bp 2104373536, has a variation type of C / T; Marker No. 19 is located on chromosome 2, bp 2134120172, and the variant type is T / C; Marker No. 20, which is located on chromosome 2, bp 2139741917, has a variation type of T / C; Marker No. 21, which is located on chromosome 2, bp 2140969969, and the variant type is A / G; Marker No. 22, which is located on chromosome 2, bp 2141004076, has a variant type of A / G; Marker No. 23, which is located on chromosome 2, bp 2144312732, has a variation type of C / G; Marker No. 24 is located on chromosome 2, bp 2155951920, and the variant type is C / T; Marker No. 25, which is located on chromosome 2, bp 2168814238, has a variant type of G / A; Marker No. 26, which is located on chromosome 2, bp 2171030095, and the variant type is T / A; Marker No. 27, which is located on chromosome 2, bp 2173296480, and the variant type is A / G; Marker No. 28, which is located on chromosome 2, bp 2175131148, has a variation type of C / G; Marker No. 29, which is located on chromosome 2, bp 2175229826, has a variation type of T / C; Marker No. 30, which is located on chromosome 2, bp 2177772806, has a variant type of A / C; Marker No. 31, which is located on chromosome 2, bp 2184028384, and the variant type is G / A; Marker No. 32, which is located on chromosome 2, bp 2184558347, has a variation type of T / C; Marker No. 33, which is located on chromosome 2, bp 2185183473, and the variant type is T / A; Marker No. 34 is located on chromosome 2, bp 2186374217, and the variant type is C / T; Marker 35, located at bp 37077949 on chromosome 3, has a variant type of C / A; Marker 36, which is located on chromosome 3, bp 39467662, with a variant type of A / G; Marker No. 37, which is located on chromosome 3, bp 323482912, has a variant type of G / A; Marker No. 38, which is located on chromosome 3, bp 333226223, has a variant type of G / A; Marker 39, which is located on chromosome 3, bp 333957544, has a variant type of A / G; Marker No. 40, which is located on chromosome 3, bp 334035592, has a variant type of A / G; Marker No. 41, which is located on chromosome 3, bp 344675912, and the variant type is C / T; Marker No. 42, which is located on chromosome 3, bp 349598945, and the variant type is A / G; Marker No. 43, which is located on chromosome 3, bp 349967597, and the variant type is A / G; Marker No. 44 is located on chromosome 3, bp 350712941, and the variant type is G / T; Marker 45 is located on chromosome 3, bp 355,447,906, and the variant type is A / G; Marker No. 46, which is located on chromosome 3, bp 356509055, and the variant type is C / T; Marker 47, which is located on chromosome 3, bp 357193563, has a variation type of T / C; Marker No. 48, which is located on chromosome 3, bp 363014229, has a variant type of G / A; Marker No. 49, which is located on chromosome 3, bp 364932466, has a variant type of G / A; Marker No. 50, which is located on chromosome 3, bp 368304699, has a variation type of C / T; Marker No. 51, which is located on chromosome 3, bp 3160699507, has a variant type of G / A; Marker No. 52, which is located on chromosome 3, bp 3161671764, has a variant type of G / A; Marker No. 53, which is located on chromosome 3, bp 3168627960, and the variant type is T / C; Marker No. 54 is located on chromosome 3, bp 3170406066, and the variant type is C / T; Marker No. 55, which is located on chromosome 3, bp 3171148457, has a variation type of T / C; Marker No. 56, which is located on chromosome 3, bp 3171572870, and the variant type is T / A; Marker 57, which is located on chromosome 3, bp 3173329295, has a variation type of T / C; Marker 58, which is located on chromosome 3, bp 3181396863, has a variation type of C / T; Marker 59, which is located on chromosome 3, bp 3185748145, has a variation type of T / A; Marker No. 60, which is located on chromosome 4, bp 43488085, and the variant type is A / G; Marker No. 61, which is located on chromosome 4, bp 49185469, has a variation type of T / C; Marker No. 62, which is located on chromosome 4, bp 420370567, has a variation type of T / C; Marker No. 63, which is located on chromosome 4, bp 422158504, and the variant type is G / A; Marker No. 64 is located on chromosome 4, bp 493029245, and the variant type is A / G; Marker No. 65 is located on chromosome 4, bp 4133737637, and the variant type is T / A; Marker No. 66, which is located on chromosome 4, bp 4136800305, and the variant type is T / C; Marker 67, which is located on chromosome 4, bp 4140123972, has a variant type of A / C; Marker No. 68, which is located on chromosome 4, bp 4142148415, and the variant type is T / G; Marker No. 69, which is located on chromosome 4, bp 4150103302, and the variant type is T / C; Marker 70 is located on chromosome 4, bp 4150752420, and the variant type is A / G; Marker No. 71, which is located on chromosome 4, bp 4151876715, and the variant type is G / A; Marker No. 72, which is located on chromosome 4, bp 4153027020, and the variant type is G / A; Marker No. 73, which is located on chromosome 4, bp 4154635908, and the variant type is C / T; Marker No. 74 is located on chromosome 4, bp 4157572576, and the variant type is G / T; Marker 75 is located on chromosome 4 at bp 4162608762, and the variant type is G / C; Marker 76, which is located on chromosome 4, bp 4163952706, has a variation type of T / C; Marker 77, which is located on chromosome 4, bp 4164312912, has a variant type of G / A; Marker 78, which is located on chromosome 4, bp 4167462144, has a variation type of T / C; Marker 79 is located on chromosome 4, bp 4174584167, and the variant type is T / C; Marker No. 80 is located on chromosome 4, bp 4175575716, and the variant type is T / A; Marker No. 81, which is located on chromosome 4, bp 4179391103, and the variant type is A / G; Marker No. 82, which is located on chromosome 5, bp 5101002, has a variant type of C / A; Marker No. 83, which is located on chromosome 5, bp 54050477, and the variant type is T / C; Marker No. 84 is located on chromosome 5, bp 56210415, and the variant type is C / T; Marker 85, which is located on chromosome 5, bp 57353166, has a variation type of T / C; Marker No. 86, which is located on chromosome 5, bp 57907806, and the variant type is T / G; Marker 87, which is located on chromosome 5, bp 58122046, has a variation type of C / T; Marker 88, which is located on chromosome 5, bp 512139653, has a variant type of A / G; Marker No. 89, which is located on chromosome 5, bp 512139824, has a variation type of T / C; Marker No. 90, which is located on chromosome 5, bp 513420754, has a variation type of T / C; Marker No. 91, which is located on chromosome 5, bp 516715793, and the variant type is C / T; Marker No. 92, which is located on chromosome 5, bp 521548705, and the variant type is A / G; Marker No. 93, which is located on chromosome 5, bp 522923012, and the variant type is T / C; Marker No. 94 is located on chromosome 5, bp 524040590, and the variant type is T / C; Marker No. 95, which is located on chromosome 5, bp 527470277, has a variation type of T / C; Marker 96, which is located on chromosome 5, bp 530565269, has a variant type of A / C; Marker 97 is located on chromosome 5, bp 531991099, and the variant type is A / T; Marker No. 98, which is located on chromosome 5, bp 535018979, has a variant type of G / T; Marker No. 99, which is located on chromosome 5, bp 536993723, has a variant type of G / T; Marker No. 100, which is located on chromosome 5, bp 537746327, has a variant type of G / A; Marker No. 101, which is located on chromosome 5, bp 543138430, and the variant type is T / C; Marker No. 102, which is located on chromosome 5, bp 543944445, and the variant type is C / T; Marker No. 103, which is located on chromosome 5, bp 550429114, and the variant type is T / C; Marker No. 104, which is located on chromosome 5, bp 561180240, and the variant type is A / G; Marker No. 105, which is located on chromosome 5, bp 565131503, has a variant type of G / A; Marker No. 106 is located on chromosome 5, bp 570663061, and the variant type is A / T; Marker No. 107, which is located on chromosome 5, bp 5144968891, and the variant type is C / T; Marker No. 108, which is located on chromosome 5, bp 5144969368, and the variant type is T / A; Marker No. 109, which is located on chromosome 5, bp 5155396989, has a variant type of A / C; Marker No. 110 is located on chromosome 5, bp 5161684131, and the variant type is C / A; Marker No. 111 is located on chromosome 5, bp 5161685301, and the variant type is T / C; Marker No. 112, which is located on chromosome 5, bp 5164032398, and the variant type is A / T; Marker No. 113, which is located on chromosome 5, bp 5164856997, with a variant type of A / G; Marker No. 114 is located on chromosome 6, bp 6778804, and the variant type is T / C; Marker No. 115 is located on chromosome 6, bp 62344276, and the variant type is G / A; Marker No. 116, which is located on chromosome 6, bp 63607324, and the variant type is G / A; Marker No. 117, which is located on chromosome 6, bp 69812751, and the variant type is C / G; Marker No. 118, which is located on chromosome 6, bp 615808467, has a variation type of C / T; Marker No. 119 is located on chromosome 6, bp 617634309, and the variant type is G / A; Marker No. 120, which is located on chromosome 6, bp 622461159, has a variant type of A / T; Marker No. 121, which is located on chromosome 6, bp 626460149, has a variant type of G / A; Marker No. 122, which is located on chromosome 6, bp 627819611, and the variant type is T / C; Marker No. 123, which is located on chromosome 6, bp 636016335, and the variant type is G / T; Marker No. 124 is located on chromosome 6, bp 6142497880, and the variant type is C / A; Marker No. 125, which is located on chromosome 6, bp 6142498639, has a variant type of G / T; Marker No. 126, which is located on chromosome 6, bp 6143663591, and the variant type is A / G; Marker No. 127, which is located on chromosome 6, bp 6145585715, and the variant type is A / C; Marker No. 128, which is located on chromosome 6, bp 6149622668, has a variant type of G / A; Marker No. 129, which is located on chromosome 6, bp 6157761880, and the variant type is C / G; Marker No. 130, which is located on chromosome 6, bp 6170724270, has a variation type of C / G; Marker No. 131, which is located on chromosome 6, bp 6170763361, and the variant type is A / G; Marker No. 132, which is located on chromosome 7, bp 71710306, and the variant type is T / C; Marker No. 133, which is located on chromosome 7, bp 73264083, and the variant type is C / G; Marker No. 134, which is located on chromosome 7, bp 77548057, has a variant type of A / C; Marker No. 135, which is located on chromosome 7, bp 77750449, has a variation type of T / A; Marker No. 136, which is located on chromosome 7, bp 712649262, and the variant type is T / A; Marker 137, which is located on chromosome 7, bp 712909310, has a variant type of A / G; Marker No. 138, which is located on chromosome 7, bp 712989315, and the variant type is C / G; Marker No. 139, which is located on chromosome 7, bp 713199095, and the variant type is T / C; Marker No. 140, which is located on chromosome 7, bp 714493374, and the variant type is C / G; Marker No. 141, which is located on chromosome 7, bp 718532969, has a variation type of C / G; Marker No. 142, which is located on chromosome 7, bp 723461275, and the variant type is C / T; Marker No. 143, which is located on chromosome 7, bp 729251772, and the variant type is C / T; Marker No. 144 is located on chromosome 7, bp 737148816, and the variant type is C / T; Marker No. 145, which is located on chromosome 7, bp 747094940, has a variation type of T / C; Marker No. 146, which is located on chromosome 7, bp 751336679, has a variant type of C / A; Marker 147, which is located on chromosome 7, bp 7162732167, has a variant type of A / G; Marker No. 148, which is located on chromosome 8, bp 81371158, with a variant type of A / G; Marker No. 149, which is located on chromosome 8, bp 86789650, and the variant type is G / A; Marker No. 150 is located on chromosome 8, bp 88437771, and the variant type is T / C; Marker No. 151, which is located on chromosome 8, bp 814588094, and the variant type is G / A; Marker No. 152, which is located on chromosome 8, bp 815736361, and the variant type is A / G; Marker No. 153, which is located on chromosome 8, bp 825810325, and the variant type is G / C; Marker No. 154 is located on chromosome 8, bp 826325818, and the variant type is T / G; Marker No. 155 is located on chromosome 8, bp 837475933, and the variant type is T / C; Marker No. 156, which is located on chromosome 8, bp 874738903, and the variant type is A / G; Marker No. 157, which is located on chromosome 8, bp 8138002896, has a variation type of C / T; Marker No. 158, which is located on chromosome 8, bp 8142021696, has a variant type of C / A; Marker No. 159, which is located on chromosome 8, bp 8145208971, and the variant type is C / T; Marker 160 is located on chromosome 8, bp 8146286654, and the variant type is A / G; Marker No. 161, which is located on chromosome 8, bp 8150404881, and the variant type is T / A; Marker No. 162, which is located on chromosome 8, bp 8151594514, and the variant type is T / C; Marker No. 163, which is located on chromosome 9, bp 91438383, and the variant type is C / T; Marker No. 164 is located on chromosome 9, bp 911449710, and the variant type is T / C; Marker No. 165, which is located on chromosome 9, bp 913479358, has a variant type of G / C; Marker No. 166, which is located on chromosome 9, bp 913489579, has a variation type of C / G; Marker No. 167, which is located on chromosome 9, bp 941147490, has a variant type of A / C; Marker No. 168, which is located on chromosome 9, bp 9141925303, and the variant type is T / C; Marker No. 169, which is located on chromosome 9, bp 9142775138, and the variant type is T / C; Marker No. 170, which is located on chromosome 9, 9147110013bp, and the variant type is C / T; Marker No. 171 is located on chromosome 9, bp 9147846861, and the variant type is G / A; Marker No. 172, which is located on chromosome 9, bp 9151353465, and the variant type is C / T; Marker No. 173, which is located on chromosome 9, bp 9153453729, and the variant type is T / C; Marker No. 174 is located on chromosome 9, bp 9153698062, and the variant type is T / A; Marker No. 175, which is located on chromosome 9, bp 9154824375, has a variation type of C / G; Marker No. 176, which is located on chromosome 9, bp 9155548198, and the variant type is A / G; Marker No. 177, which is located on chromosome 9, bp 9155548252, and the variant type is G / A; Marker No. 178, which is located on chromosome 9, bp 9155576669, has a variation type of C / G; Marker 179 is located on chromosome 10, bp 102302094, and the variant type is G / C; Marker 180 is located on chromosome 10, bp 109432633, and the variant type is T / C; Marker 181 is located on chromosome 10, bp 1017416902, and the variant type is A / T; Marker No. 182, which is located on chromosome 10, bp 10121954875, and the variant type is G / A; Marker 183 is located on chromosome 10, bp 10130315170, and the variant type is A / T; Marker 184 is located on chromosome 10, 10132041600bp, and the variant type is G / T; Marker No. 185 is located on chromosome 10, bp 10137888718, and the variant type is C / T; Marker No. 186, which is located on chromosome 10, bp 10142022816, has a variant type of A / G; Marker No. 187, which is located on chromosome 10, bp 10143214786, has a variant type of G / A; Marker No. 188, which is located on chromosome 10, bp 10143714885, and the variant type is A / G; Marker No. 189 is located on chromosome 10, bp 10144007893, and the variant type is C / T; Marker No. 190, which is located on chromosome 11, 1155,339,362 bp, has a variant type of G / A; Marker No. 191, which is located on chromosome 11, 1190715627bp, has a variant type of A / G; Marker No. 192, which is located on chromosome 11, 1194,597,680 bp, has a variation type of T / G; Marker No. 193 is located on chromosome 11, bp 1194839888, and the variant type is A / T; Marker No. 194 is located on chromosome 11, 1195,818,114 bp, and the variant type is T / C; Marker No. 195, which is located on chromosome 11, bp 11105015249, has a variant type of G / A; Marker No. 196, which is located on chromosome 11, bp 11118023633, and the variant type is C / A; Marker No. 197, which is located on chromosome 11, 1128125475bp, has a variant type of A / G; Marker No. 198, which is located on chromosome 11, bp 11133467339, has a variant type of G / A; Marker No. 199 is located on chromosome 11, 1138493485 bp, and the variant type is C / T; Marker No. 200 is located on chromosome 12, bp 123684982, and the variant type is G / A; Marker No. 201 is located on chromosome 12, bp 125,995,960, and the variant type is G / A; Marker No. 202, which is located on chromosome 12, bp 126835408, and the variant type is T / C; Marker No. 203, which is located on chromosome 12, bp 1213550741, and the variant type is T / C; Marker No. 204 is located on chromosome 12, bp 1213899205, and the variant type is A / C; Marker No. 205 is located on chromosome 12, bp 1214447869, and the variant type is G / A; Marker No. 206, which is located on chromosome 12, 12107960617bp, has a variation type of T / G; Marker No. 207, which is located on chromosome 12, bp 12119478235, has a variation type of T / C; Marker No. 208, which is located on chromosome 12, 12120018613bp, and the variant type is C / T; Marker No. 209 is located on chromosome 12, bp 12124086849, and the variant type is G / T; Marker No. 210 is located on chromosome 12, bp 12124453241, and the variant type is G / A; Marker No. 211 is located on chromosome 12, bp 12128376164, and the variant type is T / G; Marker No. 212, which is located on chromosome 12, bp 12128559402, has a variant type of G / A; Marker No. 213, which is located on chromosome 12, bp 12128953055, and the variant type is C / T; Marker No. 214 is located on chromosome 12, bp 12130582077, and the variant type is A / G; Marker 215 is located on chromosome 12, bp 12132475469, and the variant type is A / G; Marker 216, which is located on chromosome 12, bp 12134372645, has a variant type of A / C; Marker No. 217 is located on chromosome 12, bp 12138491493, and the variant type is C / T; Marker No. 218, which is located on chromosome 12, 12140124081bp, has a variation type of C / G; Marker No. 219 is located on chromosome 12, bp 12140239381, and the variant type is G / T; Marker No. 220, which is located on chromosome 13, bp 1327030687, has a variant type of A / G; Marker No. 221 is located on chromosome 13, bp 1334684042, and the variant type is C / T; Marker No. 222, which is located on chromosome 13, bp 1345894210, and the variant type is C / T; Marker No. 223 is located on chromosome 13, bp 1353577453, and the variant type is A / G; Marker No. 224 is located on chromosome 13, bp 1353812335, and the variant type is A / G; Marker No. 225 is located on chromosome 13, bp 1360489824, and the variant type is T / G; Marker No. 226, which is located on chromosome 13, bp 1367836217, and the variant type is C / T; Marker No. 227, which is located on chromosome 13, bp 1373891012, and the variant type is T / C; Marker No. 228, which is located on chromosome 13, bp 1376533867, with a variant type of A / G; Marker No. 229, which is located on chromosome 13, bp 1383098051, and the variant type is T / C; Marker No. 230, which is located on chromosome 13, bp 1388748630, and the variant type is G / A; Marker No. 231 is located on chromosome 13, bp 1389057411, and the variant type is C / T; Marker No. 232, which is located on chromosome 13, bp 1390232542, with a variant type of A / G; Marker No. 233, which is located on chromosome 13, bp 1390395203, and the variant type is A / G; Marker No. 234 is located on chromosome 14, bp 1465570441, and the variant type is G / A; Marker No. 235, which is located on chromosome 14, bp 1469425337, has a variant type of A / G; Marker No. 236 is located on chromosome 14, bp 1475905772, and the variant type is G / A; Marker No. 237 is located on chromosome 14, bp 1478843002, and the variant type is T / C; Marker No. 238, which is located on chromosome 14, bp 1481865290, and the variant type is C / T; Marker No. 239 is located on chromosome 14, bp 1481945414, and the variant type is A / T; Marker No. 240 is located on chromosome 14, bp 1484299928, and the variant type is C / T; Marker No. 241, which is located on chromosome 14, bp 1485726361, and the variant type is G / A; Marker No. 242 is located on chromosome 14, bp 1488144162, and the variant type is G / A; Marker No. 243 is located on chromosome 14, bp 1489143409, and the variant type is G / A; Marker No. 244 is located on chromosome 15, bp 153,183,570, and the variant type is C / T; Marker No. 245 is located on chromosome 15, bp 154250994, and the variant type is G / A; Marker No. 246, which is located on chromosome 15, bp 157960137, and the variant type is A / G; Marker 247 is located on chromosome 15, bp 1512731347, and the variant type is A / G; Marker No. 248 is located on chromosome 15, bp 1515374666, and the variant type is C / T; Marker No. 249 is located on chromosome 15, bp 1520140486, and the variant type is T / G; Marker No. 250 is located on chromosome 15, bp 1526989810, and the variant type is A / T; Marker No. 251 is located on chromosome 15, bp 1585734438, and the variant type is C / T; Marker No. 252 is located on chromosome 16, bp 1632401183, and the variant type is G / A; Marker No. 253 is located on chromosome 16, bp 1,649,669,601, and the variant type is T / C; Marker No. 254 is located on chromosome 16, bp 1656467304, and the variant type is A / G; Marker No. 255 is located on chromosome 16, 1662417000bp, and the variant type is G / T; Marker No. 256 is located on chromosome 16, bp 1665186178, and the variant type is A / G; Marker No. 257 is located on chromosome 16, bp 1667185586, and the variant type is A / G; Marker No. 258 is located on chromosome 16, bp 1668159645, and the variant type is T / C; Marker No. 259 is located on chromosome 16, bp 1670703024, and the variant type is G / A; Marker No. 260 is located on chromosome 16, bp 1674142244, and the variant type is T / G; The name of the dwarf coconut reference genome is: cocos_nucifera_dwarf.genome.fa.

2. Application of SNP marker combination in classification and identification of coconut germplasm resources, the SNP marker combination includes 260 SNP markers located on chromosomes Chr01 to Chr16, and the SNP markers include: Marker No. 1, which is located on chromosome 1, bp 11,190,781, and the variant type is T / G; Marker No. 2, which is located on chromosome 1 at bp 12235818, has a variant type of G / A; Marker No. 3, which is located on chromosome 1, bp 17980352, and the variant type is A / G; Marker No. 4, which is located on chromosome 1, bp 110680094, and the variant type is A / G; Marker No. 5, which is located on chromosome 1, bp 144832968, has a variation type of C / G; Marker No. 6, which is located on chromosome 1, bp 146014481, and the variant type is T / A; Marker No. 7, which is located on chromosome 1, bp 146793777, and the variant type is A / G; Marker No. 8, which is located on chromosome 1, bp 154417159, has a variation type of C / T; Marker No. 9, which is located on chromosome 1, bp 157934494, and the variant type is A / G; Marker No. 10 is located on chromosome 1, bp 159488786, and the variant type is A / G; Marker No. 11 is located on chromosome 1 at bp 1177659160, and the variant type is A / G; Marker No. 12, which is located on chromosome 1, bp 1205295810, and the variant type is C / T; Marker No. 13 is located on chromosome 1, bp 1209649951, and the variant type is G / A; Marker No. 14 is located on chromosome 1, bp 1210539345, and the variant type is T / C; Marker No. 15, which is located on chromosome 1, bp 1213678628, has a variation type of T / C; Marker No. 16, which is located on chromosome 2, bp 25511596, and the variant type is A / T; Marker 17 is located on chromosome 2, bp 242777399, and the variant type is T / C; Marker 18, which is located on chromosome 2, bp 2104373536, has a variation type of C / T; Marker No. 19 is located on chromosome 2, bp 2134120172, and the variant type is T / C; Marker No. 20, which is located on chromosome 2, bp 2139741917, has a variation type of T / C; Marker No. 21, which is located on chromosome 2, bp 2140969969, and the variant type is A / G; Marker No. 22, which is located on chromosome 2, bp 2141004076, has a variant type of A / G; Marker No. 23, which is located on chromosome 2, bp 2144312732, has a variation type of C / G; Marker No. 24 is located on chromosome 2, bp 2155951920, and the variant type is C / T; Marker No. 25, which is located on chromosome 2, bp 2168814238, has a variant type of G / A; Marker No. 26, which is located on chromosome 2, bp 2171030095, and the variant type is T / A; Marker No. 27, which is located on chromosome 2, bp 2173296480, and the variant type is A / G; Marker No. 28, which is located on chromosome 2, bp 2175131148, has a variation type of C / G; Marker No. 29, which is located on chromosome 2, bp 2175229826, has a variation type of T / C; Marker No. 30, which is located on chromosome 2, bp 2177772806, has a variant type of A / C; Marker No. 31, which is located on chromosome 2, bp 2184028384, and the variant type is G / A; Marker No. 32, which is located on chromosome 2, bp 2184558347, has a variation type of T / C; Marker No. 33, which is located on chromosome 2, bp 2185183473, and the variant type is T / A; Marker No. 34 is located on chromosome 2, bp 2186374217, and the variant type is C / T; Marker 35, located at bp 37077949 on chromosome 3, has a variant type of C / A; Marker 36, which is located on chromosome 3, bp 39467662, with a variant type of A / G; Marker No. 37, which is located on chromosome 3, bp 323482912, has a variant type of G / A; Marker No. 38, which is located on chromosome 3, bp 333226223, has a variant type of G / A; Marker 39, which is located on chromosome 3, bp 333957544, has a variant type of A / G; Marker No. 40, which is located on chromosome 3, bp 334035592, has a variant type of A / G; Marker No. 41, which is located on chromosome 3, bp 344675912, and the variant type is C / T; Marker No. 42, which is located on chromosome 3, bp 349598945, and the variant type is A / G; Marker No. 43, which is located on chromosome 3, bp 349967597, and the variant type is A / G; Marker No. 44 is located on chromosome 3, bp 350712941, and the variant type is G / T; Marker 45 is located on chromosome 3, bp 355,447,906, and the variant type is A / G; Marker No. 46, which is located on chromosome 3, bp 356509055, and the variant type is C / T; Marker 47, which is located on chromosome 3, bp 357193563, has a variation type of T / C; Marker No. 48, which is located on chromosome 3, bp 363014229, has a variant type of G / A; Marker No. 49, which is located on chromosome 3, bp 364932466, has a variant type of G / A; Marker No. 50, which is located on chromosome 3, bp 368304699, has a variation type of C / T; Marker No. 51, which is located on chromosome 3, bp 3160699507, has a variant type of G / A; Marker No. 52, which is located on chromosome 3, bp 3161671764, has a variant type of G / A; Marker No. 53, which is located on chromosome 3, bp 3168627960, and the variant type is T / C; Marker No. 54 is located on chromosome 3, bp 3170406066, and the variant type is C / T; Marker No. 55, which is located on chromosome 3, bp 3171148457, has a variation type of T / C; Marker No. 56, which is located on chromosome 3, bp 3171572870, and the variant type is T / A; Marker 57, which is located on chromosome 3, bp 3173329295, has a variation type of T / C; Marker 58, which is located on chromosome 3, bp 3181396863, has a variation type of C / T; Marker 59, which is located on chromosome 3, bp 3185748145, has a variation type of T / A; Marker No. 60, which is located on chromosome 4, bp 43488085, and the variant type is A / G; Marker No. 61, which is located on chromosome 4, bp 49185469, has a variation type of T / C; Marker No. 62, which is located on chromosome 4, bp 420370567, has a variation type of T / C; Marker No. 63, which is located on chromosome 4, bp 422158504, and the variant type is G / A; Marker No. 64 is located on chromosome 4, bp 493029245, and the variant type is A / G; Marker No. 65 is located on chromosome 4, bp 4133737637, and the variant type is T / A; Marker No. 66, which is located on chromosome 4, bp 4136800305, and the variant type is T / C; Marker 67, which is located on chromosome 4, bp 4140123972, has a variant type of A / C; Marker No. 68, which is located on chromosome 4, bp 4142148415, and the variant type is T / G; Marker No. 69, which is located on chromosome 4, bp 4150103302, and the variant type is T / C; Marker 70 is located on chromosome 4, bp 4150752420, and the variant type is A / G; Marker No. 71, which is located on chromosome 4, bp 4151876715, and the variant type is G / A; Marker No. 72, which is located on chromosome 4, bp 4153027020, and the variant type is G / A; Marker No. 73, which is located on chromosome 4, bp 4154635908, and the variant type is C / T; Marker No. 74 is located on chromosome 4, bp 4157572576, and the variant type is G / T; Marker 75 is located on chromosome 4 at bp 4162608762, and the variant type is G / C; Marker 76, which is located on chromosome 4, bp 4163952706, has a variation type of T / C; Marker 77, which is located on chromosome 4, bp 4164312912, has a variant type of G / A; Marker 78, which is located on chromosome 4, bp 4167462144, has a variation type of T / C; Marker 79 is located on chromosome 4, bp 4174584167, and the variant type is T / C; Marker No. 80 is located on chromosome 4, bp 4175575716, and the variant type is T / A; Marker No. 81, which is located on chromosome 4, bp 4179391103, and the variant type is A / G; Marker No. 82, which is located on chromosome 5, bp 5101002, has a variant type of C / A; Marker No. 83, which is located on chromosome 5, bp 54050477, and the variant type is T / C; Marker No. 84 is located on chromosome 5, bp 56210415, and the variant type is C / T; Marker 85, which is located on chromosome 5, bp 57353166, has a variation type of T / C; Marker No. 86, which is located on chromosome 5, bp 57907806, and the variant type is T / G; Marker 87, which is located on chromosome 5, bp 58122046, has a variation type of C / T; Marker 88, which is located on chromosome 5, bp 512139653, has a variant type of A / G; Marker No. 89, which is located on chromosome 5, bp 512139824, has a variation type of T / C; Marker No. 90, which is located on chromosome 5, bp 513420754, has a variation type of T / C; Marker No. 91, which is located on chromosome 5, bp 516715793, and the variant type is C / T; Marker No. 92, which is located on chromosome 5, bp 521548705, and the variant type is A / G; Marker No. 93, which is located on chromosome 5, bp 522923012, and the variant type is T / C; Marker No. 94 is located on chromosome 5, bp 524040590, and the variant type is T / C; Marker No. 95, which is located on chromosome 5, bp 527470277, has a variation type of T / C; Marker 96, which is located on chromosome 5, bp 530565269, has a variant type of A / C; Marker 97 is located on chromosome 5, bp 531991099, and the variant type is A / T; Marker No. 98, which is located on chromosome 5, bp 535018979, has a variant type of G / T; Marker No. 99, which is located on chromosome 5, bp 536993723, has a variant type of G / T; Marker No. 100, which is located on chromosome 5, bp 537746327, has a variant type of G / A; Marker No. 101, which is located on chromosome 5, bp 543138430, and the variant type is T / C; Marker No. 102, which is located on chromosome 5, bp 543944445, and the variant type is C / T; Marker No. 103, which is located on chromosome 5, bp 550429114, and the variant type is T / C; Marker No. 104, which is located on chromosome 5, bp 561180240, and the variant type is A / G; Marker No. 105, which is located on chromosome 5, bp 565131503, has a variant type of G / A; Marker No. 106 is located on chromosome 5, bp 570663061, and the variant type is A / T; Marker No. 107, which is located on chromosome 5, bp 5144968891, and the variant type is C / T; Marker No. 108, which is located on chromosome 5, bp 5144969368, and the variant type is T / A; Marker No. 109, which is located on chromosome 5, bp 5155396989, has a variant type of A / C; Marker No. 110 is located on chromosome 5, bp 5161684131, and the variant type is C / A; Marker No. 111 is located on chromosome 5, bp 5161685301, and the variant type is T / C; Marker No. 112, which is located on chromosome 5, bp 5164032398, and the variant type is A / T; Marker No. 113, which is located on chromosome 5, bp 5164856997, with a variant type of A / G; Marker No. 114 is located on chromosome 6, bp 6778804, and the variant type is T / C; Marker No. 115 is located on chromosome 6, bp 62344276, and the variant type is G / A; Marker No. 116, which is located on chromosome 6, bp 63607324, and the variant type is G / A; Marker No. 117, which is located on chromosome 6, bp 69812751, and the variant type is C / G; Marker No. 118, which is located on chromosome 6, bp 615808467, has a variation type of C / T; Marker No. 119 is located on chromosome 6, bp 617634309, and the variant type is G / A; Marker No. 120, which is located on chromosome 6, bp 622461159, has a variant type of A / T; Marker No. 121, which is located on chromosome 6, bp 626460149, has a variant type of G / A; Marker No. 122, which is located on chromosome 6, bp 627819611, and the variant type is T / C; Marker No. 123, which is located on chromosome 6, bp 636016335, and the variant type is G / T; Marker No. 124 is located on chromosome 6, bp 6142497880, and the variant type is C / A; Marker No. 125, which is located on chromosome 6, bp 6142498639, has a variant type of G / T; Marker No. 126, which is located on chromosome 6, bp 6143663591, and the variant type is A / G; Marker No. 127, which is located on chromosome 6, bp 6145585715, and the variant type is A / C; Marker No. 128, which is located on chromosome 6, bp 6149622668, has a variant type of G / A; Marker No. 129, which is located on chromosome 6, bp 6157761880, and the variant type is C / G; Marker No. 130, which is located on chromosome 6, bp 6170724270, has a variation type of C / G; Marker No. 131, which is located on chromosome 6, bp 6170763361, and the variant type is A / G; Marker No. 132, which is located on chromosome 7, bp 71710306, and the variant type is T / C; Marker No. 133, which is located on chromosome 7, bp 73264083, and the variant type is C / G; Marker No. 134, which is located on chromosome 7, bp 77548057, has a variant type of A / C; Marker No. 135, which is located on chromosome 7, bp 77750449, has a variation type of T / A; Marker No. 136, which is located on chromosome 7, bp 712649262, and the variant type is T / A; Marker 137, which is located on chromosome 7, bp 712909310, has a variant type of A / G; Marker No. 138, which is located on chromosome 7, bp 712989315, and the variant type is C / G; Marker No. 139, which is located on chromosome 7, bp 713199095, and the variant type is T / C; Marker No. 140, which is located on chromosome 7, bp 714493374, and the variant type is C / G; Marker No. 141, which is located on chromosome 7, bp 718532969, has a variation type of C / G; Marker No. 142, which is located on chromosome 7, bp 723461275, and the variant type is C / T; Marker No. 143, which is located on chromosome 7, bp 729251772, and the variant type is C / T; Marker No. 144 is located on chromosome 7, bp 737148816, and the variant type is C / T; Marker No. 145, which is located on chromosome 7, bp 747094940, has a variation type of T / C; Marker No. 146, which is located on chromosome 7, bp 751336679, has a variant type of C / A; Marker 147, which is located on chromosome 7, bp 7162732167, has a variant type of A / G; Marker No. 148, which is located on chromosome 8, bp 81371158, with a variant type of A / G; Marker No. 149, which is located on chromosome 8, bp 86789650, and the variant type is G / A; Marker No. 150 is located on chromosome 8, bp 88437771, and the variant type is T / C; Marker No. 151, which is located on chromosome 8, bp 814588094, and the variant type is G / A; Marker No. 152, which is located on chromosome 8, bp 815736361, and the variant type is A / G; Marker No. 153, which is located on chromosome 8, bp 825810325, and the variant type is G / C; Marker No. 154 is located on chromosome 8, bp 826325818, and the variant type is T / G; Marker No. 155 is located on chromosome 8, bp 837475933, and the variant type is T / C; Marker No. 156, which is located on chromosome 8, bp 874738903, and the variant type is A / G; Marker No. 157, which is located on chromosome 8, bp 8138002896, has a variation type of C / T; Marker No. 158, which is located on chromosome 8, bp 8142021696, has a variant type of C / A; Marker No. 159, which is located on chromosome 8, bp 8145208971, and the variant type is C / T; Marker 160 is located on chromosome 8, bp 8146286654, and the variant type is A / G; Marker No. 161, which is located on chromosome 8, bp 8150404881, and the variant type is T / A; Marker No. 162, which is located on chromosome 8, bp 8151594514, and the variant type is T / C; Marker No. 163, which is located on chromosome 9, bp 91438383, and the variant type is C / T; Marker No. 164 is located on chromosome 9, bp 911449710, and the variant type is T / C; Marker No. 165, which is located on chromosome 9, bp 913479358, has a variant type of G / C; Marker No. 166, which is located on chromosome 9, bp 913489579, has a variation type of C / G; Marker No. 167, which is located on chromosome 9, bp 941147490, has a variant type of A / C; Marker No. 168, which is located on chromosome 9, bp 9141925303, and the variant type is T / C; Marker No. 169, which is located on chromosome 9, bp 9142775138, and the variant type is T / C; Marker No. 170, which is located on chromosome 9, 9147110013bp, and the variant type is C / T; Marker No. 171 is located on chromosome 9, bp 9147846861, and the variant type is G / A; Marker No. 172, which is located on chromosome 9, bp 9151353465, and the variant type is C / T; Marker No. 173, which is located on chromosome 9, bp 9153453729, and the variant type is T / C; Marker No. 174 is located on chromosome 9, bp 9153698062, and the variant type is T / A; Marker No. 175, which is located on chromosome 9, bp 9154824375, has a variation type of C / G; Marker No. 176, which is located on chromosome 9, bp 9155548198, and the variant type is A / G; Marker No. 177, which is located on chromosome 9, bp 9155548252, and the variant type is G / A; Marker No. 178, which is located on chromosome 9, bp 9155576669, has a variation type of C / G; Marker 179 is located on chromosome 10, bp 102302094, and the variant type is G / C; Marker 180 is located on chromosome 10, bp 109432633, and the variant type is T / C; Marker 181 is located on chromosome 10, bp 1017416902, and the variant type is A / T; Marker No. 182, which is located on chromosome 10, bp 10121954875, and the variant type is G / A; Marker 183 is located on chromosome 10, bp 10130315170, and the variant type is A / T; Marker 184 is located on chromosome 10, 10132041600bp, and the variant type is G / T; Marker No. 185 is located on chromosome 10, bp 10137888718, and the variant type is C / T; Marker No. 186, which is located on chromosome 10, bp 10142022816, has a variant type of A / G; Marker No. 187, which is located on chromosome 10, bp 10143214786, has a variant type of G / A; Marker No. 188, which is located on chromosome 10, bp 10143714885, and the variant type is A / G; Marker No. 189 is located on chromosome 10, bp 10144007893, and the variant type is C / T; Marker No. 190, which is located on chromosome 11, 1155,339,362 bp, has a variant type of G / A; Marker No. 191, which is located on chromosome 11, 1190715627bp, has a variant type of A / G; Marker No. 192, which is located on chromosome 11, 1194,597,680 bp, has a variation type of T / G; Marker No. 193 is located on chromosome 11, bp 1194839888, and the variant type is A / T; Marker No. 194 is located on chromosome 11, 1195,818,114 bp, and the variant type is T / C; Marker No. 195, which is located on chromosome 11, bp 11105015249, has a variant type of G / A; Marker No. 196, which is located on chromosome 11, bp 11118023633, and the variant type is C / A; Marker No. 197, which is located on chromosome 11, 1128125475bp, has a variant type of A / G; Marker No. 198, which is located on chromosome 11, bp 11133467339, has a variant type of G / A; Marker No. 199 is located on chromosome 11, 1138493485 bp, and the variant type is C / T; Marker No. 200 is located on chromosome 12, bp 123684982, and the variant type is G / A; Marker No. 201 is located on chromosome 12, bp 125,995,960, and the variant type is G / A; Marker No. 202, which is located on chromosome 12, bp 126835408, and the variant type is T / C; Marker No. 203, which is located on chromosome 12, bp 1213550741, and the variant type is T / C; Marker No. 204 is located on chromosome 12, bp 1213899205, and the variant type is A / C; Marker No. 205 is located on chromosome 12, bp 1214447869, and the variant type is G / A; Marker No. 206, which is located on chromosome 12, 12107960617bp, has a variation type of T / G; Marker No. 207, which is located on chromosome 12, bp 12119478235, has a variation type of T / C; Marker No. 208, which is located on chromosome 12, 12120018613bp, and the variant type is C / T; Marker No. 209 is located on chromosome 12, bp 12124086849, and the variant type is G / T; Marker No. 210 is located on chromosome 12, bp 12124453241, and the variant type is G / A; Marker No. 211 is located on chromosome 12, bp 12128376164, and the variant type is T / G; Marker No. 212, which is located on chromosome 12, bp 12128559402, has a variant type of G / A; Marker No. 213, which is located on chromosome 12, bp 12128953055, and the variant type is C / T; Marker No. 214 is located on chromosome 12, bp 12130582077, and the variant type is A / G; Marker 215 is located on chromosome 12, bp 12132475469, and the variant type is A / G; Marker 216, which is located on chromosome 12, bp 12134372645, has a variant type of A / C; Marker No. 217 is located on chromosome 12, bp 12138491493, and the variant type is C / T; Marker No. 218, which is located on chromosome 12, 12140124081bp, has a variation type of C / G; Marker No. 219 is located on chromosome 12, bp 12140239381, and the variant type is G / T; Marker No. 220, which is located on chromosome 13, bp 1327030687, has a variant type of A / G; Marker No. 221 is located on chromosome 13, bp 1334684042, and the variant type is C / T; Marker No. 222, which is located on chromosome 13, bp 1345894210, and the variant type is C / T; Marker No. 223 is located on chromosome 13, bp 1353577453, and the variant type is A / G; Marker No. 224 is located on chromosome 13, bp 1353812335, and the variant type is A / G; Marker No. 225 is located on chromosome 13, bp 1360489824, and the variant type is T / G; Marker No. 226, which is located on chromosome 13, bp 1367836217, and the variant type is C / T; Marker No. 227, which is located on chromosome 13, bp 1373891012, and the variant type is T / C; Marker No. 228, which is located on chromosome 13, bp 1376533867, with a variant type of A / G; Marker No. 229, which is located on chromosome 13, bp 1383098051, and the variant type is T / C; Marker No. 230, which is located on chromosome 13, bp 1388748630, and the variant type is G / A; Marker No. 231 is located on chromosome 13, bp 1389057411, and the variant type is C / T; Marker No. 232, which is located on chromosome 13, bp 1390232542, with a variant type of A / G; Marker No. 233, which is located on chromosome 13, bp 1390395203, and the variant type is A / G; Marker No. 234 is located on chromosome 14, bp 1465570441, and the variant type is G / A; Marker No. 235, which is located on chromosome 14, bp 1469425337, has a variant type of A / G; Marker No. 236 is located on chromosome 14, bp 1475905772, and the variant type is G / A; Marker No. 237 is located on chromosome 14, bp 1478843002, and the variant type is T / C; Marker No. 238, which is located on chromosome 14, bp 1481865290, and the variant type is C / T; Marker No. 239 is located on chromosome 14, bp 1481945414, and the variant type is A / T; Marker No. 240 is located on chromosome 14, bp 1484299928, and the variant type is C / T; Marker No. 241, which is located on chromosome 14, bp 1485726361, and the variant type is G / A; Marker No. 242 is located on chromosome 14, bp 1488144162, and the variant type is G / A; Marker No. 243 is located on chromosome 14, bp 1489143409, and the variant type is G / A; Marker No. 244 is located on chromosome 15, bp 153,183,570, and the variant type is C / T; Marker No. 245 is located on chromosome 15, bp 154250994, and the variant type is G / A; Marker No. 246, which is located on chromosome 15, bp 157960137, and the variant type is A / G; Marker 247 is located on chromosome 15, bp 1512731347, and the variant type is A / G; Marker No. 248 is located on chromosome 15, bp 1515374666, and the variant type is C / T; Marker No. 249 is located on chromosome 15, bp 1520140486, and the variant type is T / G; Marker No. 250 is located on chromosome 15, bp 1526989810, and the variant type is A / T; Marker No. 251 is located on chromosome 15, bp 1585734438, and the variant type is C / T; Marker No. 252 is located on chromosome 16, bp 1632401183, and the variant type is G / A; Marker No. 253 is located on chromosome 16, bp 1,649,669,601, and the variant type is T / C; Marker No. 254 is located on chromosome 16, bp 1656467304, and the variant type is A / G; Marker No. 255 is located on chromosome 16, 1662417000bp, and the variant type is G / T; Marker No. 256 is located on chromosome 16, bp 1665186178, and the variant type is A / G; Marker No. 257 is located on chromosome 16, bp 1667185586, and the variant type is A / G; Marker No. 258 is located on chromosome 16, bp 1668159645, and the variant type is T / C; Marker No. 259 is located on chromosome 16, bp 1670703024, and the variant type is G / A; Marker No. 260 is located on chromosome 16, bp 1674142244, and the variant type is T / G; The name of the dwarf coconut reference genome is: cocos_nucifera_dwarf.genome.fa.

3. A method for identifying coconut germplasm using a combination of SNP markers, characterized in that: The SNP marker combination includes 260 SNP markers located on chromosomes Chr01 to Chr16, and the SNP markers include: Marker No. 1, which is located on chromosome 1, bp 11,190,781, and the variant type is T / G; Marker No. 2, which is located on chromosome 1 at bp 12235818, has a variant type of G / A; Marker No. 3, which is located on chromosome 1, bp 17980352, and the variant type is A / G; Marker No. 4, which is located on chromosome 1, bp 110680094, and the variant type is A / G; Marker No. 5, which is located on chromosome 1, bp 144832968, has a variation type of C / G; Marker No. 6, which is located on chromosome 1, bp 146014481, and the variant type is T / A; Marker No. 7, which is located on chromosome 1, bp 146793777, and the variant type is A / G; Marker No. 8, which is located on chromosome 1, bp 154417159, has a variation type of C / T; Marker No. 9, which is located on chromosome 1, bp 157934494, and the variant type is A / G; Marker No. 10 is located on chromosome 1, bp 159488786, and the variant type is A / G; Marker No. 11 is located on chromosome 1 at bp 1177659160, and the variant type is A / G; Marker No. 12, which is located on chromosome 1, bp 1205295810, and the variant type is C / T; Marker No. 13 is located on chromosome 1, bp 1209649951, and the variant type is G / A; Marker No. 14 is located on chromosome 1, bp 1210539345, and the variant type is T / C; Marker No. 15, which is located on chromosome 1, bp 1213678628, has a variation type of T / C; Marker No. 16, which is located on chromosome 2, bp 25511596, and the variant type is A / T; Marker 17 is located on chromosome 2, bp 242777399, and the variant type is T / C; Marker 18, which is located on chromosome 2, bp 2104373536, has a variation type of C / T; Marker No. 19 is located on chromosome 2, bp 2134120172, and the variant type is T / C; Marker No. 20, which is located on chromosome 2, bp 2139741917, has a variation type of T / C; Marker No. 21, which is located on chromosome 2, bp 2140969969, and the variant type is A / G; Marker No. 22, which is located on chromosome 2, bp 2141004076, has a variant type of A / G; Marker No. 23, which is located on chromosome 2, bp 2144312732, has a variation type of C / G; Marker No. 24 is located on chromosome 2, bp 2155951920, and the variant type is C / T; Marker No. 25, which is located on chromosome 2, bp 2168814238, has a variant type of G / A; Marker No. 26, which is located on chromosome 2, bp 2171030095, and the variant type is T / A; Marker No. 27, which is located on chromosome 2, bp 2173296480, and the variant type is A / G; Marker No. 28, which is located on chromosome 2, bp 2175131148, has a variation type of C / G; Marker No. 29, which is located on chromosome 2, bp 2175229826, has a variation type of T / C; Marker No. 30, which is located on chromosome 2, bp 2177772806, has a variant type of A / C; Marker No. 31, which is located on chromosome 2, bp 2184028384, and the variant type is G / A; Marker No. 32, which is located on chromosome 2, bp 2184558347, has a variation type of T / C; Marker No. 33, which is located on chromosome 2, bp 2185183473, and the variant type is T / A; Marker No. 34 is located on chromosome 2, bp 2186374217, and the variant type is C / T; Marker 35, located at bp 37077949 on chromosome 3, has a variant type of C / A; Marker 36, which is located on chromosome 3, bp 39467662, with a variant type of A / G; Marker No. 37, which is located on chromosome 3, bp 323482912, has a variant type of G / A; Marker No. 38, which is located on chromosome 3, bp 333226223, has a variant type of G / A; Marker 39, which is located on chromosome 3, bp 333957544, has a variant type of A / G; Marker No. 40, which is located on chromosome 3, bp 334035592, has a variant type of A / G; Marker No. 41, which is located on chromosome 3, bp 344675912, and the variant type is C / T; Marker No. 42, which is located on chromosome 3, bp 349598945, and the variant type is A / G; Marker No. 43, which is located on chromosome 3, bp 349967597, and the variant type is A / G; Marker No. 44 is located on chromosome 3, bp 350712941, and the variant type is G / T; Marker 45 is located on chromosome 3, bp 355,447,906, and the variant type is A / G; Marker No. 46, which is located on chromosome 3, bp 356509055, and the variant type is C / T; Marker 47, which is located on chromosome 3, bp 357193563, has a variation type of T / C; Marker No. 48, which is located on chromosome 3, bp 363014229, has a variant type of G / A; Marker No. 49, which is located on chromosome 3, bp 364932466, has a variant type of G / A; Marker No. 50, which is located on chromosome 3, bp 368304699, has a variation type of C / T; Marker No. 51, which is located on chromosome 3, bp 3160699507, has a variant type of G / A; Marker No. 52, which is located on chromosome 3, bp 3161671764, has a variant type of G / A; Marker No. 53, which is located on chromosome 3, bp 3168627960, and the variant type is T / C; Marker No. 54 is located on chromosome 3, bp 3170406066, and the variant type is C / T; Marker No. 55, which is located on chromosome 3, bp 3171148457, has a variation type of T / C; Marker No. 56, which is located on chromosome 3, bp 3171572870, and the variant type is T / A; Marker 57, which is located on chromosome 3, bp 3173329295, has a variation type of T / C; Marker 58, which is located on chromosome 3, bp 3181396863, has a variation type of C / T; Marker 59, which is located on chromosome 3, bp 3185748145, has a variation type of T / A; Marker No. 60, which is located on chromosome 4, bp 43488085, and the variant type is A / G; Marker No. 61, which is located on chromosome 4, bp 49185469, has a variation type of T / C; Marker No. 62, which is located on chromosome 4, bp 420370567, has a variation type of T / C; Marker No. 63, which is located on chromosome 4, bp 422158504, and the variant type is G / A; Marker No. 64 is located on chromosome 4, bp 493029245, and the variant type is A / G; Marker No. 65 is located on chromosome 4, bp 4133737637, and the variant type is T / A; Marker No. 66, which is located on chromosome 4, bp 4136800305, and the variant type is T / C; Marker 67, which is located on chromosome 4, bp 4140123972, has a variant type of A / C; Marker No. 68, which is located on chromosome 4, bp 4142148415, and the variant type is T / G; Marker No. 69, which is located on chromosome 4, bp 4150103302, and the variant type is T / C; Marker 70 is located on chromosome 4, bp 4150752420, and the variant type is A / G; Marker No. 71, which is located on chromosome 4, bp 4151876715, and the variant type is G / A; Marker No. 72, which is located on chromosome 4, bp 4153027020, and the variant type is G / A; Marker No. 73, which is located on chromosome 4, bp 4154635908, and the variant type is C / T; Marker No. 74 is located on chromosome 4, bp 4157572576, and the variant type is G / T; Marker 75 is located on chromosome 4 at bp 4162608762, and the variant type is G / C; Marker 76, which is located on chromosome 4, bp 4163952706, has a variation type of T / C; Marker 77, which is located on chromosome 4, bp 4164312912, has a variant type of G / A; Marker 78, which is located on chromosome 4, bp 4167462144, has a variation type of T / C; Marker 79 is located on chromosome 4, bp 4174584167, and the variant type is T / C; Marker No. 80 is located on chromosome 4, bp 4175575716, and the variant type is T / A; Marker No. 81, which is located on chromosome 4, bp 4179391103, and the variant type is A / G; Marker No. 82, which is located on chromosome 5, bp 5101002, has a variant type of C / A; Marker No. 83, which is located on chromosome 5, bp 54050477, and the variant type is T / C; Marker No. 84 is located on chromosome 5, bp 56210415, and the variant type is C / T; Marker 85, which is located on chromosome 5, bp 57353166, has a variation type of T / C; Marker No. 86, which is located on chromosome 5, bp 57907806, and the variant type is T / G; Marker 87, which is located on chromosome 5, bp 58122046, has a variation type of C / T; Marker 88, which is located on chromosome 5, bp 512139653, has a variant type of A / G; Marker No. 89, which is located on chromosome 5, bp 512139824, has a variation type of T / C; Marker No. 90, which is located on chromosome 5, bp 513420754, has a variation type of T / C; Marker No. 91, which is located on chromosome 5, bp 516715793, and the variant type is C / T; Marker No. 92, which is located on chromosome 5, bp 521548705, and the variant type is A / G; Marker No. 93, which is located on chromosome 5, bp 522923012, and the variant type is T / C; Marker No. 94 is located on chromosome 5, bp 524040590, and the variant type is T / C; Marker No. 95, which is located on chromosome 5, bp 527470277, has a variation type of T / C; Marker 96, which is located on chromosome 5, bp 530565269, has a variant type of A / C; Marker 97 is located on chromosome 5, bp 531991099, and the variant type is A / T; Marker No. 98, which is located on chromosome 5, bp 535018979, has a variant type of G / T; Marker No. 99, which is located on chromosome 5, bp 536993723, has a variant type of G / T; Marker No. 100, which is located on chromosome 5, bp 537746327, has a variant type of G / A; Marker No. 101, which is located on chromosome 5, bp 543138430, and the variant type is T / C; Marker No. 102, which is located on chromosome 5, bp 543944445, and the variant type is C / T; Marker No. 103, which is located on chromosome 5, bp 550429114, and the variant type is T / C; Marker No. 104, which is located on chromosome 5, bp 561180240, and the variant type is A / G; Marker No. 105, which is located on chromosome 5, bp 565131503, has a variant type of G / A; Marker No. 106 is located on chromosome 5, bp 570663061, and the variant type is A / T; Marker No. 107, which is located on chromosome 5, bp 5144968891, and the variant type is C / T; Marker No. 108, which is located on chromosome 5, bp 5144969368, and the variant type is T / A; Marker No. 109, which is located on chromosome 5, bp 5155396989, has a variant type of A / C; Marker No. 110 is located on chromosome 5, bp 5161684131, and the variant type is C / A; Marker No. 111 is located on chromosome 5, bp 5161685301, and the variant type is T / C; Marker No. 112, which is located on chromosome 5, bp 5164032398, and the variant type is A / T; Marker No. 113, which is located on chromosome 5, bp 5164856997, with a variant type of A / G; Marker No. 114 is located on chromosome 6, bp 6778804, and the variant type is T / C; Marker No. 115 is located on chromosome 6, bp 62344276, and the variant type is G / A; Marker No. 116, which is located on chromosome 6, bp 63607324, and the variant type is G / A; Marker No. 117, which is located on chromosome 6, bp 69812751, and the variant type is C / G; Marker No. 118, which is located on chromosome 6, bp 615808467, has a variation type of C / T; Marker No. 119 is located on chromosome 6, bp 617634309, and the variant type is G / A; Marker No. 120, which is located on chromosome 6, bp 622461159, has a variant type of A / T; Marker No. 121, which is located on chromosome 6, bp 626460149, has a variant type of G / A; Marker No. 122, which is located on chromosome 6, bp 627819611, and the variant type is T / C; Marker No. 123, which is located on chromosome 6, bp 636016335, and the variant type is G / T; Marker No. 124 is located on chromosome 6, bp 6142497880, and the variant type is C / A; Marker No. 125, which is located on chromosome 6, bp 6142498639, has a variant type of G / T; Marker No. 126, which is located on chromosome 6, bp 6143663591, and the variant type is A / G; Marker No. 127, which is located on chromosome 6, bp 6145585715, and the variant type is A / C; Marker No. 128, which is located on chromosome 6, bp 6149622668, has a variant type of G / A; Marker No. 129, which is located on chromosome 6, bp 6157761880, and the variant type is C / G; Marker No. 130, which is located on chromosome 6, bp 6170724270, has a variation type of C / G; Marker No. 131, which is located on chromosome 6, bp 6170763361, and the variant type is A / G; Marker No. 132, which is located on chromosome 7, bp 71710306, and the variant type is T / C; Marker No. 133, which is located on chromosome 7, bp 73264083, and the variant type is C / G; Marker No. 134, which is located on chromosome 7, bp 77548057, has a variant type of A / C; Marker No. 135, which is located on chromosome 7, bp 77750449, has a variation type of T / A; Marker No. 136, which is located on chromosome 7, bp 712649262, and the variant type is T / A; Marker 137, which is located on chromosome 7, bp 712909310, has a variant type of A / G; Marker No. 138, which is located on chromosome 7, bp 712989315, and the variant type is C / G; Marker No. 139, which is located on chromosome 7, bp 713199095, and the variant type is T / C; Marker No. 140, which is located on chromosome 7, bp 714493374, and the variant type is C / G; Marker No. 141, which is located on chromosome 7, bp 718532969, has a variation type of C / G; Marker No. 142, which is located on chromosome 7, bp 723461275, and the variant type is C / T; Marker No. 143, which is located on chromosome 7, bp 729251772, and the variant type is C / T; Marker No. 144 is located on chromosome 7, bp 737148816, and the variant type is C / T; Marker No. 145, which is located on chromosome 7, bp 747094940, has a variation type of T / C; Marker No. 146, which is located on chromosome 7, bp 751336679, has a variant type of C / A; Marker 147, which is located on chromosome 7, bp 7162732167, has a variant type of A / G; Marker No. 148, which is located on chromosome 8, bp 81371158, with a variant type of A / G; Marker No. 149, which is located on chromosome 8, bp 86789650, and the variant type is G / A; Marker No. 150 is located on chromosome 8, bp 88437771, and the variant type is T / C; Marker No. 151, which is located on chromosome 8, bp 814588094, and the variant type is G / A; Marker No. 152, which is located on chromosome 8, bp 815736361, and the variant type is A / G; Marker No. 153, which is located on chromosome 8, bp 825810325, and the variant type is G / C; Marker No. 154 is located on chromosome 8, bp 826325818, and the variant type is T / G; Marker No. 155 is located on chromosome 8, bp 837475933, and the variant type is T / C; Marker No. 156, which is located on chromosome 8, bp 874738903, and the variant type is A / G; Marker No. 157, which is located on chromosome 8, bp 8138002896, has a variation type of C / T; Marker No. 158, which is located on chromosome 8, bp 8142021696, has a variant type of C / A; Marker No. 159, which is located on chromosome 8, bp 8145208971, and the variant type is C / T; Marker 160 is located on chromosome 8, bp 8146286654, and the variant type is A / G; Marker No. 161, which is located on chromosome 8, bp 8150404881, and the variant type is T / A; Marker No. 162, which is located on chromosome 8, bp 8151594514, and the variant type is T / C; Marker No. 163, which is located on chromosome 9, bp 91438383, and the variant type is C / T; Marker No. 164 is located on chromosome 9, bp 911449710, and the variant type is T / C; Marker No. 165, which is located on chromosome 9, bp 913479358, has a variant type of G / C; Marker No. 166, which is located on chromosome 9, bp 913489579, has a variation type of C / G; Marker No. 167, which is located on chromosome 9, bp 941147490, has a variant type of A / C; Marker No. 168, which is located on chromosome 9, bp 9141925303, and the variant type is T / C; Marker No. 169, which is located on chromosome 9, bp 9142775138, and the variant type is T / C; Marker No. 170, which is located on chromosome 9, 9147110013bp, and the variant type is C / T; Marker No. 171 is located on chromosome 9, bp 9147846861, and the variant type is G / A; Marker No. 172, which is located on chromosome 9, bp 9151353465, and the variant type is C / T; Marker No. 173, which is located on chromosome 9, bp 9153453729, and the variant type is T / C; Marker No. 174 is located on chromosome 9, bp 9153698062, and the variant type is T / A; Marker No. 175, which is located on chromosome 9, bp 9154824375, has a variation type of C / G; Marker No. 176, which is located on chromosome 9, bp 9155548198, and the variant type is A / G; Marker No. 177, which is located on chromosome 9, bp 9155548252, and the variant type is G / A; Marker No. 178, which is located on chromosome 9, bp 9155576669, has a variation type of C / G; Marker 179 is located on chromosome 10, bp 102302094, and the variant type is G / C; Marker 180 is located on chromosome 10, bp 109432633, and the variant type is T / C; Marker 181 is located on chromosome 10, bp 1017416902, and the variant type is A / T; Marker No. 182, which is located on chromosome 10, bp 10121954875, and the variant type is G / A; Marker 183 is located on chromosome 10, bp 10130315170, and the variant type is A / T; Marker 184 is located on chromosome 10, 10132041600bp, and the variant type is G / T; Marker No. 185 is located on chromosome 10, bp 10137888718, and the variant type is C / T; Marker No. 186, which is located on chromosome 10, bp 10142022816, has a variant type of A / G; Marker No. 187, which is located on chromosome 10, bp 10143214786, has a variant type of G / A; Marker No. 188, which is located on chromosome 10, bp 10143714885, and the variant type is A / G; Marker No. 189 is located on chromosome 10, bp 10144007893, and the variant type is C / T; Marker No. 190, which is located on chromosome 11, 1155,339,362 bp, has a variant type of G / A; Marker No. 191, which is located on chromosome 11, 1190715627bp, has a variant type of A / G; Marker No. 192, which is located on chromosome 11, 1194,597,680 bp, has a variation type of T / G; Marker No. 193 is located on chromosome 11, bp 1194839888, and the variant type is A / T; Marker No. 194 is located on chromosome 11, 1195,818,114 bp, and the variant type is T / C; Marker No. 195, which is located on chromosome 11, bp 11105015249, has a variant type of G / A; Marker No. 196, which is located on chromosome 11, bp 11118023633, and the variant type is C / A; Marker No. 197, which is located on chromosome 11, 1128125475bp, has a variant type of A / G; Marker No. 198, which is located on chromosome 11, bp 11133467339, has a variant type of G / A; Marker No. 199 is located on chromosome 11, 1138493485 bp, and the variant type is C / T; Marker No. 200 is located on chromosome 12, bp 123684982, and the variant type is G / A; Marker No. 201 is located on chromosome 12, bp 125,995,960, and the variant type is G / A; Marker No. 202, which is located on chromosome 12, bp 126835408, and the variant type is T / C; Marker No. 203, which is located on chromosome 12, bp 1213550741, and the variant type is T / C; Marker No. 204 is located on chromosome 12, bp 1213899205, and the variant type is A / C; Marker No. 205 is located on chromosome 12, bp 1214447869, and the variant type is G / A; Marker No. 206, which is located on chromosome 12, 12107960617bp, has a variation type of T / G; Marker No. 207, which is located on chromosome 12, bp 12119478235, has a variation type of T / C; Marker No. 208, which is located on chromosome 12, 12120018613bp, and the variant type is C / T; Marker No. 209 is located on chromosome 12, bp 12124086849, and the variant type is G / T; Marker No. 210 is located on chromosome 12, bp 12124453241, and the variant type is G / A; Marker No. 211 is located on chromosome 12, bp 12128376164, and the variant type is T / G; Marker No. 212, which is located on chromosome 12, bp 12128559402, has a variant type of G / A; Marker No. 213, which is located on chromosome 12, bp 12128953055, and the variant type is C / T; Marker No. 214 is located on chromosome 12, bp 12130582077, and the variant type is A / G; Marker 215 is located on chromosome 12, bp 12132475469, and the variant type is A / G; Marker 216, which is located on chromosome 12, bp 12134372645, has a variant type of A / C; Marker No. 217 is located on chromosome 12, bp 12138491493, and the variant type is C / T; Marker No. 218, which is located on chromosome 12, 12140124081bp, has a variation type of C / G; Marker No. 219 is located on chromosome 12, bp 12140239381, and the variant type is G / T; Marker No. 220, which is located on chromosome 13, bp 1327030687, has a variant type of A / G; Marker No. 221 is located on chromosome 13, bp 1334684042, and the variant type is C / T; Marker No. 222, which is located on chromosome 13, bp 1345894210, and the variant type is C / T; Marker No. 223 is located on chromosome 13, bp 1353577453, and the variant type is A / G; Marker No. 224 is located on chromosome 13, bp 1353812335, and the variant type is A / G; Marker No. 225 is located on chromosome 13, bp 1360489824, and the variant type is T / G; Marker No. 226, which is located on chromosome 13, bp 1367836217, and the variant type is C / T; Marker No. 227, which is located on chromosome 13, bp 1373891012, and the variant type is T / C; Marker No. 228, which is located on chromosome 13, bp 1376533867, with a variant type of A / G; Marker No. 229, which is located on chromosome 13, bp 1383098051, and the variant type is T / C; Marker No. 230, which is located on chromosome 13, bp 1388748630, and the variant type is G / A; Marker No. 231 is located on chromosome 13, bp 1389057411, and the variant type is C / T; Marker No. 232, which is located on chromosome 13, bp 1390232542, with a variant type of A / G; Marker No. 233, which is located on chromosome 13, bp 1390395203, and the variant type is A / G; Marker No. 234 is located on chromosome 14, bp 1465570441, and the variant type is G / A; Marker No. 235, which is located on chromosome 14, bp 1469425337, has a variant type of A / G; Marker No. 236 is located on chromosome 14, bp 1475905772, and the variant type is G / A; Marker No. 237 is located on chromosome 14, bp 1478843002, and the variant type is T / C; Marker No. 238, which is located on chromosome 14, bp 1481865290, and the variant type is C / T; Marker No. 239 is located on chromosome 14, bp 1481945414, and the variant type is A / T; Marker No. 240 is located on chromosome 14, bp 1484299928, and the variant type is C / T; Marker No. 241, which is located on chromosome 14, bp 1485726361, and the variant type is G / A; Marker No. 242 is located on chromosome 14, bp 1488144162, and the variant type is G / A; Marker No. 243 is located on chromosome 14, bp 1489143409, and the variant type is G / A; Marker No. 244 is located on chromosome 15, bp 153,183,570, and the variant type is C / T; Marker No. 245 is located on chromosome 15, bp 154250994, and the variant type is G / A; Marker No. 246, which is located on chromosome 15, bp 157960137, and the variant type is A / G; Marker 247 is located on chromosome 15, bp 1512731347, and the variant type is A / G; Marker No. 248 is located on chromosome 15, bp 1515374666, and the variant type is C / T; Marker No. 249 is located on chromosome 15, bp 1520140486, and the variant type is T / G; Marker No. 250 is located on chromosome 15, bp 1526989810, and the variant type is A / T; Marker No. 251 is located on chromosome 15, bp 1585734438, and the variant type is C / T; Marker No. 252 is located on chromosome 16, bp 1632401183, and the variant type is G / A; Marker No. 253 is located on chromosome 16, bp 1,649,669,601, and the variant type is T / C; Marker No. 254 is located on chromosome 16, bp 1656467304, and the variant type is A / G; Marker No. 255 is located on chromosome 16, 1662417000bp, and the variant type is G / T; Marker No. 256 is located on chromosome 16, bp 1665186178, and the variant type is A / G; Marker No. 257 is located on chromosome 16, bp 1667185586, and the variant type is A / G; Marker No. 258 is located on chromosome 16, bp 1668159645, and the variant type is T / C; Marker No. 259 is located on chromosome 16, bp 1670703024, and the variant type is G / A; Marker No. 260 is located on chromosome 16, bp 1674142244, and the variant type is T / G; The steps include: (1) Extracting DNA from coconut samples; (2) Sequencing the genome of individual species of coconut sample DNA; (3) Genotyping was performed based on the sequencing results compared with the dwarf coconut reference genome to identify the SNP marker information of each variety; (4) Determine the similarity between the sample to be tested and the known fingerprint sample based on the genotype combination of the known fingerprint SNP markers, thereby evaluating the variety and source information of the coconut; The name of the dwarf coconut reference genome is: cocos_nucifera_dwarf.genome.fa.

4. The method according to claim 3, characterized in that The coconut samples are fresh coconut leaves.

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