Method for breaking cistanche deserticola seed dormancy and improving germination rate

Abstract

The invention relates to the technical field of agricultural planting, in particular to a method for breaking dormancy of cistanche seeds and improving germination rate, which comprises the following steps: pretreating the cistanche seeds for 24 hours by using 10mg / L fluridone, and then culturing the cistanche seeds by using 25mg / L gibberellin solution. According to the method for breaking the dormancy of the cistanche deserticola seeds and improving the germination rate, the cistanche deserticola seeds are pretreated with fluridone of 10 mg / L for 24 hours, then the germination effect of the cistanche deserticola seeds cultured with a gibberellin solution of 25 mg / L is optimal, and the germination rate of the cistanche deserticola seeds is the highest and is 59.43%.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, in particular to a method for breaking the dormancy of Cistanche deserticola seeds and improving the germination rate. Background Art

[0002] The morphological characteristics of Cistanche deserticola are perennial parasitic herbs, 40-160 cm tall, with leaves densely packed at the bottom of the stem, broadly ovate or triangular ovate, inflorescence 15-50 cm long, corolla light yellow, lobes light yellow or lavender, brown when dry, the stem of Cistanche deserticola is fleshy, single or branched, with many leaves, scaly, spirally arranged, light yellowish white, corolla tubular bell-shaped, flowers yellow or lavender, turning brown when dry.

[0003] The existing method for improving the germination rate (publication number CN201410749636.6) has an uneven mixing method, which makes the germination rate unstable, and ultimately causes the problems of low sowing germination rate and uneven germination, making it difficult to promote and apply it. In order to protect the resources of Cistanche deserticola and provide more Cistanche deserticola medicinal materials for the market to meet the needs, there is an urgent need for a method that is simpler to operate, easier to master, and more valuable for promotion and application to improve the germination rate of Cistanche deserticola seeds. Summary of the invention

[0004] The purpose of the present invention is to provide a method for breaking the dormancy of Cistanche deserticola seeds and improving the germination rate, so as to solve the problem that the uneven mixing in the prior art proposed in the above background technology makes the germination rate unstable, which makes it difficult to promote and apply. To achieve the above purpose, the present invention provides the following technical scheme: a method for breaking the dormancy of Cistanche deserticola seeds and improving the germination rate, pre-treating Cistanche deserticola seeds with 10 mg / L fluridone for 24 hours, and then culturing the Cistanche deserticola seeds with 25 mg / L gibberellin solution, comprising the following steps:

[0005] S1: First, rinse the Cistanche deserticola seeds with 75% ethanol solution for 15 seconds, and use sterile filter paper to absorb the moisture on the surface of the Cistanche deserticola seeds, in order to disinfect the pre-treated seeds and kill some bacteria on the surface of the seeds;

[0006] S2: Soak the Cistanche deserticola seeds in distilled water at 25-30°C for four hours;

[0007] S3: The Cistanche deserticola seeds were pretreated with 10 mg / L fluridone for 24 hours, and then cultured with 25 mg / L gibberellin solution.

[0008] Further preferably, the culture dish filter paper method is used for dark culture in a constant temperature incubator at 25° C., the culture solution is replaced every 3 days, the germination status is recorded after 7 days, and the seeds are considered to have germinated if germ tubes are formed and the length of the germ tubes is greater than 1 mm. The culture period is 30 days.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] In the present invention, the Cistanche deserticola seeds were pretreated with 10 mg / L fluridone for 24 hours and then cultured with 25 mg / L gibberellin solution, and the germination effect of the Cistanche deserticola seeds was the best, and the germination rate of the Cistanche deserticola seeds was the highest, which was 59.43%. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a schematic diagram of the process of the present invention. DETAILED DESCRIPTION

[0012] Embodiment 1:

[0013] Rinse the Cistanche deserticola seeds with 75% ethanol solution for 15 seconds, and use sterile filter paper to absorb the moisture on the surface of the Cistanche deserticola seeds;

[0014] At 25-30°C, the seeds were first soaked in distilled water for 4 hours, and then pretreated with 5 fluridone mass concentration gradients (10, 20, 30, 40, 50 mg / L) for 24 hours. The seeds were pretreated with distilled water as a control. The sterilized seeds were inoculated in a culture dish, with 100 seeds in each dish, and repeated three times.

[0015] The culture dish filter paper method was used to culture the seeds in a constant temperature incubator at 25°C in the dark. The culture medium was replaced every 3 days. The germination status was recorded after 7 days. Seeds that formed germ tubes and whose length was greater than 1 mm were considered to have germinated. The culture period was 30 days. There was a significant difference between the pretreatments with fluridone and distilled water (P < 0.01).

[0016] Embodiment 2:

[0017] Rinse the Cistanche deserticola seeds with 75% ethanol solution for 15 seconds, and use sterile filter paper to absorb the moisture on the surface of the Cistanche deserticola seeds;

[0018] At 25-30℃, the seeds were first soaked in distilled water for 4 hours, and then pretreated with 5 aqueous solutions with 5 gibberellin mass concentration gradients (5, 25, 35, 45, 55 mg / L) for 5 hours. The seeds were pretreated with distilled water as a control. The sterilized seeds were inoculated in culture dishes, with 100 seeds in each dish, and repeated three times.

[0019] The culture dish filter paper method was used to culture the seeds in a constant temperature incubator at 25℃ in the dark. The culture medium was replaced every 3 days. The germination status was recorded after 7 days. Seeds were considered to have germinated when they formed germ tubes and the length of the germ tubes was greater than 1mm. The culture period was 30 days. After pretreatment with fluridone, the germination rate of Cistanche seeds cultured in gibberellin solution did not increase significantly, and there was no significant difference among the treatments. The highest germination rate was only 7.34%.

[0020] Embodiment 3:

[0021] Rinse the Cistanche deserticola seeds with 75% ethanol solution for 15 seconds, and use sterile filter paper to absorb the moisture on the surface of the Cistanche deserticola seeds;

[0022] At 25-30 ℃, the seeds were first soaked in distilled water for 4 hours, and 50 mg / L fluridone solution was used as the solvent, and the gibberellin culture solution was prepared. Five treatments were set with a mass concentration gradient (10, 20, 30, 40, 50 mg / L). The 50 mg / L fluridone solution was used as the control. The sterilized seeds were inoculated in a culture dish, and 100 seeds were inoculated in each culture dish. The process was repeated three times. After fluridone pretreatment, the germination rate of Cistanche deserticola seeds cultured in gibberellin solution did not increase significantly, and there was no significant difference among the treatments. The highest germination rate was only 7.34%, and the fluridone solution was used as the control. The difference between the pretreatment with distilled water was extremely significant (P < 0.01), indicating that the seeds of Cistanche deserticola pretreated with fluridone and then treated with gibberellin had a significant promoting effect on the germination of Cistanche deserticola seeds. In the process of germination of Cistanche deserticola seeds synergistically induced by fluridone and gibberellin, fluridone pretreatment can effectively release the dormancy of Cistanche deserticola seeds. Moreover, the germination effect of Cistanche deserticola seeds was best when they were pretreated with 10 mg / L fluridone for 24 hours and then cultured with 25 mg / L gibberellin solution, and the germination rate of Cistanche deserticola seeds was the highest, at 59.43%.

[0023] The above shows and describes the basic principles, main features and advantages of the present invention. Technical personnel in this industry should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for breaking the dormancy of Cistanche deserticola seeds and increasing the germination rate, It is characterized in that The method comprises the following steps: pre-treating Cistanche deserticola seeds with 10 mg / L fluridone for 24 hours and then culturing the Cistanche deserticola seeds with 25 mg / L gibberellin solution: S1: First, rinse the Cistanche deserticola seeds with 75% ethanol solution for 15 seconds, and use sterile filter paper to absorb the moisture on the surface of the Cistanche deserticola seeds, in order to disinfect the pre-treated seeds and kill some bacteria on the surface of the seeds; S2: Soak the Cistanche deserticola seeds in distilled water at 25-30°C for four hours; S3: The Cistanche deserticola seeds were pretreated with 10 mg / L fluridone for 24 hours, and then cultured with 25 mg / L gibberellin solution.

2. A method for breaking the dormancy of Cistanche deserticola seeds and increasing the germination rate according to claim 1, Features: The culture dish filter paper method was used for dark culture in a constant temperature incubator at 25°C. The culture medium was replaced every 3 days. The germination status was recorded after 7 days. Seeds were considered to have germinated when germ tubes formed and the length of the germ tubes was greater than 1 mm. The culture period was 30 days.

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