Propagation method of seven-flower seeds

Abstract

The invention discloses a seven-seed flower seed propagation method, which belongs to the technical field of seed propagation, and comprises the steps of pruning and flower thinning, fertilizing in a flowering period, seed collection, seed screening, low-temperature treatment, substrate selection and sterilization, hormone treatment before germination, seed disinfection, seed germination, seedling management, seedling transplantation and the like. The method is beneficial to improvement of development of seed embryos of the paris polyphylla, increase of seed germination rate, germination potential and germination index, increase of survival rate of seedlings and promotion of growth of the seedlings, and a beneficial method can be provided for large-area popularization and planting of the paris polyphylla.

Description

Technical Field

[0001] The invention belongs to the technical field of plant propagation, and in particular relates to a seed propagation method of an endangered plant Heptaphyllum sibiricum. Background Art

[0002] Seven-flower Heptacodium miconioides Rehd.) belongs to the Caprifoliaceae family, a deciduous small tree, generally up to 7 meters tall; the bark is gray-brown and flaky; the young branches are slightly quadrangular and reddish-brown. It is a monotypic genus of Tertiary relicts unique to my country, the first batch of second-level protected plants in the country, and is listed as a highly endangered species in the key groups of Chinese angiosperms and a priority species in the China Diversity Conservation Action Plan. It has high ornamental and scientific research value, and is also of great value in studying the systematic development of Caprifoliaceae plants, glacial climate change, and the endangered mechanism of relict plants. Seven-son flower is currently only distributed in Xingshan County, Hubei, Tiantai Mountain and Siming Mountain, Zhejiang, Beishan, Yiwu, Dapan Mountain, Jinhua, Tangjiawan, Changhua, and a few areas in Jingxian and Xuancheng, Anhui. The existing resources are very few, and it is a highly endangered plant.

[0003] At present, the research on seven-son flower mainly focuses on the cultivation of plant adults and seedlings, and there are not many studies on what factors affect its seed germination. In the future, the protection of endangered plants should be formulated according to their own genetic characteristics and external factors. Therefore, in order to protect the genetic diversity of endangered plants, the study of seed germination is particularly important. With the continuous changes in the growth environment, plant growth and physiological characteristics are also slowly changing. Although there are many research results on the factors affecting seed germination of other types of plants, there are not many studies on the factors affecting the germination of seven-son flower seeds.

[0004] At present, under natural conditions, due to the influence of factors such as temperature and light, the germination rate of seven-son flower seeds is very low, and the number of seven-son flowers reproduced by natural means is very small. Therefore, the number of seven-son flowers in nature is also very small.

[0005] The applicant has used cuttings to carry out asexual reproduction of seven-son flower. The survival rate of seven-son flower is relatively high, which is conducive to increasing the number of seven-son flower and has a beneficial effect on preventing the extinction of endangered plants. However, since asexual reproduction cannot change its genetic genes, its germplasm cannot be updated.

[0006] During the seed propagation process of Seven-son Flower, the traditional propagation method is used, but the germination effect of the seeds of Seven-son Flower is not good during the seed germination process, and the seed germination rate is still very low. Therefore, it is urgent to solve the problem of what kind of germination method to choose, what kind of seed treatment method to use, and what kind of germination environmental factors to determine in order to improve the germination rate of Seven-son Flower seeds, improve the survival rate of seedlings, facilitate the reproduction of Seven-son Flower, increase the number of Seven-son Flower plants, and provide effective methods for the protection, introduction and cultivation of the endangered plant Seven-son Flower. Summary of the invention

[0007] The purpose of the present invention is to overcome the technical problems in the prior art that the traditional germination methods cannot solve the low seed germination rate and seed reproduction survival rate of seven-son flower, and to provide a seven-son flower seed reproduction method that can effectively improve the seed germination rate of seven-son flower, improve the seed reproduction survival rate, and help increase the number of seven-son flower plants. The endangered plant seven-son flower can be effectively protected, and an effective method can be provided for the introduction and cultivation of seven-son flower.

[0008] In order to solve the above technical problems, the present invention provides a method for propagating seven-son flower seeds, comprising the following steps: (1) Pruning and thinning flowers: In July, in the distribution area of ​​the seven-song flower population, the branches of the seven-song flower are pruned and the tops are removed in the early flowering period to remove some of the excess flowers. The seven-song flower has a long flowering period and blooms more. It consumes too much nutrients during flowering. In all aspects of the reproduction of the seven-song flower, the inability to effectively set fruit is the fundamental factor hindering the development of the seven-song flower population. The seven-song flower has a low fruit setting rate and incomplete embryo development. When the fruit falls off, most seeds have only heart-shaped or horseshoe-shaped embryos, and the embryos are not fully mature, resulting in an extremely low seed germination rate. As a result, the seven-song flower lacks seedlings in natural communities and there are very few saplings. The present invention reduces nutrient consumption by removing some of the excess flowers and improves the seed setting rate by topping.

[0009] (2) Add fertilizer during the flowering period: During the peak flowering period of the seven-song flower, apply more phosphorus fertilizer to promote embryo development and maturation; because phosphorus fertilizer can promote the growth and development of the seven-song flower, it can also promote the differentiation of flower buds, shorten the time of flower bud differentiation, and promote the ripening of the seven-song flower fruit.

[0010] (3) Seed collection: Collect plump seeds with purple or dark red calyx from late October to early November and set aside. Studies have shown that during the seed germination process, selecting Heptaphyllum seeds from Heptaphyllum populations in natural environments has an important influence on the germination rate, germination potential and germination index of seed germination, which is beneficial to optimize germplasm resources.

[0011] (4) Seed selection: Select fresh seeds with a single seed weight of 2-4g, a length of 10-13cm, and a diameter of 2-3cm, soak them in sterilizing solution for 2-3h, and then wash them with sterile water. Seven-spotted flowers bloom a lot, but the fruit setting rate is low. Most seeds only have heart-shaped and horseshoe-shaped embryos and are not mature. Especially early-blooming flowers have a very low fruit setting rate. Seven-spotted flowers have obvious spatial advantages. Resource limitation is the main factor affecting fruit setting. Removing the top can increase the fruit setting rate of seeds. Therefore, the selection of mature seeds plays an important role in improving the germination rate of seeds.

[0012] After selecting the seeds, air-dry them indoors for 30 days to keep the moisture content of the seeds at 7% to 10%. Since seeds have a dormant period, air-drying the seeds will weaken their physiological activities and keep them in a relatively static state. This will allow the seeds to pass the dormant period and is conducive to seed germination.

[0013] (5) Low temperature treatment: The naturally air-dried seven-song flower seeds are placed in a seed box for storage. The seven-song flower seeds are treated alternately at different temperatures. First, the seven-song flower seeds are placed at a temperature of 25°C for 40 days, and then placed at a temperature of 4°C for 20 days. The temperature treatment is performed alternately two to four times. During the germination process of the seven-song flower, the alternating temperature treatment has a significant effect on the germination of the seven-song flower seeds. It can soften the seed coat, allow the seed coat and embryo to absorb water, and help improve the vitality of the seeds. Low temperature treatment is beneficial to breaking the dormancy period of the seven-song flower seeds, increasing the germination rate of the seeds, and making the seeds germinate strong. During the alternating temperature treatment, the temperature cannot be too high. If the temperature is too high, exceeding 30°C, it will inhibit the germination of the seeds.

[0014] (6) Selection and sterilization of substrate: Select a mixture of peat and coconut bran, with the mass ratio of peat and coconut bran set to 1:4~5. Select river sand, with the mass ratio of river sand to mixed soil set to 1:1~2, because when the seeds absorb water, their respiration will be enhanced and their oxygen demand will be large. The river sand laid on the mixed soil can increase the ventilation volume, which is beneficial to increase oxygen for the seeds. The selection of substrate has a certain influence on the germination of different seeds. The use of the above substrate is beneficial to the germination of seven-son flower seeds and can increase the germination rate of seeds. The above substrate for seed germination is sterilized at high temperature, with the sterilization temperature set to 120~130℃ and the sterilization time set to 2~3h. After sterilization, a 10~13cm thick layer of peat and coconut bran mixed soil is laid on the bottom of the germination container, and a 7~9cm layer of river sand is covered on the top of the mixed soil. By sterilizing the substrate, bacteria, fungi, etc. in the substrate can be prevented from causing damage to the seed sprouts.

[0015] (7) Hormone treatment before germination: The seeds of seven-song flower that have been treated with low temperature are placed in a rooting solution and soaked for 36 to 48 hours. The rooting solution consists of GA3 (gibberellic acid), 6-BA (6-benzylaminopurine) and NAA (naphthaleneacetic acid). The concentration of GA3 is 1-50 mg / L, the concentration of 6-BA is 1-4 mg / L, and the concentration of NAA is 0.2-0.4 mg / L. The mass ratio of GA3:6-BA:NAA is 200-250:5-10:1. It is found through experiments that a higher or lower concentration of a single hormone will obviously inhibit the germination of seven-song flower seeds to a certain extent. A higher concentration of mixed hormone cannot improve the germination rate of seven-song flower seeds and also has a certain inhibitory effect. The present invention uses the above-mentioned mixed hormone to promote the germination rate, germination potential and germination index of seven-song flower to a certain extent, which can make the seven-song flower seeds germinate faster and more uniformly, and can effectively improve the vitality of the seeds.

[0016] (8) Seed disinfection: Take out the seeds that have been treated with pre-germination hormones and disinfect them with 70% alcohol. Disinfection can prevent bacteria and fungi on the surface of the seeds from damaging the seed sprouts.

[0017] (9) Seed germination: Place the seeds disinfected with alcohol in a germination container containing a sterilized matrix. The seeds are placed on the surface of a mixture of peat soil and coconut bran. Cover the top of the mixed soil with wet river sand. Place the germination container in an artificial climate box with constant temperature and light for cultivation. The temperature of the artificial climate box is set at 20-25°C, the day and night lighting is 1200 Lux / total darkness, the alternation time is 14h / 10h, and the humidity is 65-85%. Keep the surface of the river sand moist. After 136 days, the seeds take root and germinate, the cotyledons unfold, and then the true leaves grow. Experiments have found that the temperature during seed germination plays a very important role in the germination of seven-song flower seeds. If the temperature is too high or too low during seed germination, it has an important impact on the germination rate of seven-song flower seeds. This may also be an important reason for the extremely low germination rate of seven-song flower seeds under natural conditions.

[0018] (10) Management during the seedling stage: After the seven-song flower grows three true leaves, the temperature of the artificial climate chamber is set to 24-27℃, the light time is set to 12-14 hours / day, and the light intensity is set to 500-1000 Lux, of which the light intensity is set to 1000 Lux from 7:00 to 10:00 in the morning, 500 Lux from 10:00 to 14:00, and 8000 Lux from 14:00 to 19:00. The relative humidity is set to 75-85%, and water is applied every other day. Since the light compensation point of the seven-song flower is high and the light saturation point is low, the ecological range of light adaptation is narrow and the adaptability to light is weak. The seven-song flower seedlings are not shade-tolerant. In nature, due to the weak light in the forest with high shade, the growth of seedlings is often at a disadvantage. The seedlings often die due to insufficient photosynthetic products caused by too weak light. Under natural conditions, the photosynthetic process of seven-son flower seedlings in a day is "double-peaked". Generally, as the temperature rises in the morning, photosynthesis gradually increases. At noon, there will be a photosynthetic "lunch break" phenomenon, and then it will gradually increase in the afternoon. The saturated light intensity of seven-son flower at 29℃ is 1000μmol·m -2 ·s or less. The seven-son flower is not tolerant to strong light. If the light intensity is too high, photoinhibition will be strengthened, causing the photosynthetic capacity to decrease. Therefore, it is necessary to imitate the natural environment in the artificial climate box. According to the growth characteristics of the seven-son flower, different light intensities are applied at different times of the day to promote photosynthesis, reduce photoinhibition, increase the net photosynthetic rate, and promote the rapid growth of the seven-son flower seedlings.

[0019] (11) Seedling transplantation: After the management during the seedling stage, when the seven-song flower grows into a seedling with a height of 20 to 40 cm, it is transplanted from the artificial climate chamber to a suitable natural environment or potted.

[0020] As a further improvement measure of the present invention, in the above-mentioned method for propagating seven-son flower seeds, during the low-temperature treatment in step (5), the temperature alternation treatment is set to be three times.

[0021] As a further improvement measure of the present invention, in the above-mentioned seven-son flower seed propagation method, in the hormone treatment before germination in the step (7), the rooting solution consists of GA3, 6-BA and NAA, the concentration of GA3 is 10-20 mg / L, the concentration of 6-BA is 2-3 mg / L, and the concentration of NAA is 0.2-0.3 mg / L; the mass ratio of GA3:6-BA:NAA is 220~230:7~8:1.

[0022] As a further improvement measure of the present invention, in the above-mentioned method for propagating seeds of Hesperus rotundus, during the germination of the seeds in step (9), the temperature of the artificial climate box is set to 25°C, because under the condition of 25°C, the germination rate, germination potential and germination index of Hesperus rotundus seeds are the highest.

[0023] As a further improvement measure of the present invention, in the above-mentioned method for propagating seven-son lily seeds, during the germination of the seeds in step (9), the humidity is set to 75-80%.

[0024] Compared with the prior art, the beneficial effects of the present invention are: 1. The embryonic development and maturation of Hesperus chinensis can be promoted by pruning and thinning flowers and fertilizing during the flowering period; 2. The germination rate is higher by collecting mature and plump seeds in the distribution area of ​​Hesperus chinensis population; 3. Alternating low-temperature treatment helps to shorten the dormancy period of Hesperus chinensis seeds and improve the germination rate of seeds; 4. Hormone treatment before germination is beneficial to improve the germination rate, germination potential and germination index of seeds; 5. By setting the matrix components in the germination container and controlling the temperature of the industrial climate box, it is beneficial to improve the germination rate of seeds; 6. Through seedling management, the survival rate of seedlings can be improved and the growth of seedlings can be promoted, which can provide a beneficial method for promoting the large-scale planting of Hesperus chinensis. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is the state of the seven-son flower seed seedlings just breaking through the soil in Example 1 of the present invention.

[0026] Figure 2 This is the state in which the cotyledons of the seven-sprout flower in Example 1 of the present invention are about to break through the surface of the sand layer.

[0027] Figure 3 This is the state in which the cotyledons of the seven-sprout plant break through the surface of the sand layer in Example 1 of the present invention.

[0028] Figure 4 This is the state in which the cotyledons of the seven-sprout plant in Example 1 of the present invention are unfolded.

[0029] Figure 5 This is the state in which the cotyledons of the seven-sprouting plant in Example 1 of the present invention turn from blue to green, and the first pair of true leaves are about to grow out.

[0030] Figure 6 This is the state in which the first pair of cotyledons of the seven-sprout flower in Example 1 of the present invention grow.

[0031] Figure 7 This is the state in which the second pair of true leaves of the seven-son flower in Example 1 of the present invention grow out.

[0032] Figure 8 This is the state in which the third pair of true leaves of the seven-son flower in Example 1 of the present invention grow out.

[0033] Fig. 9 This is the state of the seven-son flower seedlings when transplanted in Example 1 of the present invention. Implementation

[0034] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0035] On the contrary, the present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention as defined by the claims. Further, in order to make the public have a better understanding of the present invention, some specific details are described in detail in the detailed description of the present invention below. Those skilled in the art can fully understand the present invention without the description of these details.

[0036] Example 1: Figures 1 to 9As shown, a method for propagating seven-son flower seeds, the specific steps are: in July, in the seven-son flower population plot of the endangered botanical garden of Taizhou College in Linhai, Taizhou City, Zhejiang Province, in the early flowering period of the seven-son flower, the branches are trimmed, the tops are removed, and some excessive flowers are removed; in the peak flowering period of the seven-son flower, phosphorus fertilizer is added to promote embryo development and maturity; in late October, plump seeds with purple-red and dark red calyx are collected for standby use; 40 fresh weight seeds with a single grain weight of 2-4g, a length of 10-13cm, and a diameter of 2-3cm are selected, soaked in sterilizing liquid for 2h, then washed with sterile water, and naturally air-dried indoors for 30 days to keep the moisture content of the seeds at 7%; the naturally air-dried seven-son flower seeds are placed in a seed box for storage, and the seven-son flower seeds are treated alternately at different temperatures, first the seven-son flower seeds are placed in a seed box, and then ... Place the flower seeds at 25℃ for 40 days, and then place the seven-son flower seeds at 4℃ for 20 days, alternating the temperature treatments for three times; select a mixed soil of peat soil and coconut bran, with the mass ratio of peat soil and coconut bran set to 1:4, and select river sand, with the mass ratio of river sand to mixed soil set to 1:1; sterilize the above-mentioned matrix for seed germination at high temperature, with the sterilization temperature set to 120℃ and the sterilization time set to 3h. After sterilization, spread a 10cm thick mixed soil of peat soil and coconut bran on the bottom of the germination container, and cover the top of the mixed soil with 7cm of river sand; soak the seven-son flower seeds after low temperature treatment in rooting solution for 48h, the rooting solution consists of GA3, 6-BA and NAA, and the concentration of GA3 is 40 mg / L, the concentration of 6-BA is 1 mg / L, and the concentration of NAA is 0.2 mg / L; the mass ratio of GA3:6-BA:NAA is 200:5:1; the seeds treated with pre-germination hormones are taken out and disinfected with 70% alcohol; the seeds disinfected with alcohol are placed in a germination container containing a sterilized matrix, the seeds are placed on the surface of a mixed soil of peat soil and coconut bran, wet river sand is covered on the top of the mixed soil, and the germination container is placed in an artificial climate box with constant temperature and lighting for cultivation. The temperature of the artificial climate box is set to 25°C, the day and night alternating lighting is 1200 Lux / full darkness, the alternation time is 14h / 10h, and the humidity is 75%. Through the method of this embodiment 1, after 136 days, the germination rate of the seven-son flower seeds is 65%, the germination potential is 46%, and the germination index is 5.9, and the germination effect is very good.

[0037] After the seven-son flower grows three true leaves, the temperature of the artificial climate box is set to 27℃, the lighting time is set to 14 hours / day, the illumination is set to 1000 Lux from 7 am to 10 am, the illumination is set to 500 Lux from 10 am to 14 pm, and the illumination is set to 800 Lux from 14 pm to 19 pm. The relative humidity is set to 85%, and watered once every other day. When the seven-son flower grows into seedlings with a height of 20~40 cm, it is transplanted from the artificial climate room to a suitable natural environment. The seven-son flower has a high survival rate and grows well.

[0038] Embodiment 2: A method for propagating seven-son flower seeds, comprising the following specific steps: in June, at a seven-son flower population plot in the endangered botanical garden of Taizhou College, Linhai, Taizhou City, Zhejiang Province, in the early flowering period of the seven-son flower, branches were trimmed, tops were removed, and a portion of excessive flowers were removed; in the blooming period of the seven-son flower, phosphorus fertilizer was added to promote embryo maturation; in early November, plump seeds with purple-red and dark red calyx were collected for standby use; 40 fresh weight seeds with a single grain weight of 2 to 4 g, a length of 10 to 13 cm, and a diameter of 2 to 3 cm were selected, soaked in a sterilizing solution for 3 h, then washed with sterile water, and naturally air-dried indoors for 28 days to keep the moisture content of the seeds at 10%; the naturally air-dried seven-son flower seeds were placed in a seed box for storage, and the seven-son flower seeds were treated alternately at different temperatures, firstly the seven-son flower seeds were placed in a seed box, and then ... The seeds of the hibiscus were placed at a temperature of 25°C for 40 days, and then the seeds of the seven-spotted hibiscus were placed at a temperature of 4°C for 20 days, and the temperature treatment was alternated for three times; a mixed soil of peat soil and coconut bran was selected, and the mass ratio of peat soil and coconut bran was set to 1:5, and river sand was selected, and the mass ratio of river sand to mixed soil was set to 1:2; the above-mentioned matrix for seed germination was sterilized at high temperature, the sterilization temperature was set to 120°C, and the sterilization time was set to 2.5h. After sterilization, a 13cm thick mixed soil of peat soil and coconut bran was spread on the bottom of the germination container, and 9cm of river sand was covered on the top of the mixed soil; the seven-spotted hibiscus seeds treated with low temperature were placed in a rooting solution and soaked for 36h. The rooting solution consisted of GA3, 6-BA and NAA, and the concentration of GA3 was 50 mg / L, the concentration of 6-BA is 4 mg / L, and the concentration of NAA is 0.4 mg / L; the mass ratio of GA3:6-BA:NAA is 250:10:1; the seeds treated with pre-germination hormones are taken out and disinfected with 70% alcohol; the seeds disinfected with alcohol are placed in a germination container containing a sterilized matrix, the seeds are placed on the surface of a mixed soil of peat soil and coconut bran, wet river sand is covered on the upper part of the mixed soil, and the germination container is placed in an artificial climate box with constant temperature and lighting for cultivation, the temperature of the artificial climate box is set to 25°C, the day and night alternating lighting is 1200 Lux / full darkness, the alternating time is 14h / 10h, and the humidity is 65%; through the method of this embodiment 2, after 139 days, the germination rate of the seven-son flower seeds is 69%, the germination potential is 47%, and the germination index is 6.1, and the germination effect is very good.

[0039] After the seven-son flower grows three true leaves, the temperature of the artificial climate box is set to 24℃, the lighting time is set to 12 hours / day, the light intensity is set to 1000 Lux from 7 am to 10 am, the light intensity is set to 600 Lux from 10 am to 14 pm, and the light intensity is set to 700 Lux from 14 pm to 19 pm. The relative humidity is set to 85%, and watered once every other day. When the seven-son flower grows into a seedling with a height of 20~40 cm, it is transplanted from the artificial climate room to a suitable pot. The seven-son flower has a high survival rate and grows well.

[0040] Embodiment 3: A method for propagating seven-song flower seeds, comprising the following specific steps: in June, at a seven-song flower population plot in the endangered botanical garden of Taizhou College, Linhai, Taizhou City, Zhejiang Province, in the early flowering period of the seven-song flower, branches were trimmed, tops were removed, and a portion of excessive flowers were removed; in the full flowering period of the seven-song flower, phosphorus fertilizer was added to promote embryo maturation; in late October, plump seeds with purple-red and dark red calyx were collected for standby use; 40 fresh weight seeds with a single grain weight of 2 to 4 g, a length of 10 to 13 cm, and a diameter of 2 to 3 cm were selected, soaked in a sterilizing solution for 2 h, then washed with sterile water, and naturally air-dried indoors for 30 days to keep the moisture content of the seeds at about 9%; the naturally air-dried seven-song flower seeds were placed in a seed box for storage, and the seven-song flower seeds were treated alternately at different temperatures, firstly, the seven-song flower seeds were placed at a temperature of 25°C. Place it at 4°C for 40 days, and then place the seven-flower seeds at 4°C for 20 days, alternate temperature treatments, and alternate treatments twice; selection and sterilization of substrate: select a mixed soil of peat soil and coconut bran, the mass ratio of peat soil and coconut bran is set to 1:5, and select river sand, the mass ratio of river sand to mixed soil is set to 1:2; sterilize the above-mentioned substrate for seed germination using high temperature, the sterilization temperature is set to 130°C, the sterilization time is set to 2h, after sterilization, spread a 10cm thick mixed soil of peat soil and coconut bran on the bottom of the germination container, and cover the top of the mixed soil with 8cm of river sand; place the seven-flower seeds treated with low temperature in rooting solution and soak for 40h, the rooting solution consists of GA3, 6-BA and NAA, the concentration of GA3 is 30mg / L, the concentration of 6-BA is 3 mg / L, the concentration of NAA is 0.3 mg / L; the mass ratio of GA3:6-BA:NAA is 220:8:1; the seeds treated with pre-germination hormones are taken out and disinfected with 70% alcohol; the seeds disinfected with alcohol are placed in a germination container containing a sterilized matrix, the seeds are placed on the surface of a mixed soil of peat soil and coconut bran, wet river sand is covered on the upper part of the mixed soil, and the germination container is placed in an artificial climate box with constant temperature and lighting for cultivation, the temperature of the artificial climate box is set to 20°C, the day and night alternating lighting is 1200 Lux / full darkness, the alternating time is 14h / 10h, and the humidity is 80%; through the method of this embodiment 3, after 135 days, the germination rate of the seven-son flower seeds is 57%, the germination potential is 40%, and the germination index is 4.8, and the germination effect is good.

[0041] After the seven-son flower grows three true leaves, the temperature of the artificial climate box is set to 26℃, the lighting time is set to 13 hours / day, the illumination is set to 800 Lux from 7:00 to 10:00 in the morning, 500 Lux from 10:00 to 14:00, and 700 Lux from 14:00 to 19:00. The relative humidity is set to 75%, and watered once every other day. When the seven-son flower grows into seedlings with a height of 20~40cm, it is transplanted from the artificial climate room to a suitable natural environment. The seven-son flower has a high survival rate and grows well.

[0042] Through research, it was found that during the germination process of seven-single flower seeds, relevant environmental factors such as substrate, temperature, light intensity, hormones, etc. have a certain impact on the germination of seven-single flower seeds.

[0043] 1. Effects of different temperatures on the germination of seven-flowered flowers When other experimental steps are the same as those in Example 1, by adjusting the temperature of the artificial climate box, the germination rate, germination potential and germination index of the seven-flower seeds are compared under different temperature conditions. It can be seen that the germination rate, germination potential and germination index of the seven-flower seeds under different temperature conditions are quite different. A lower temperature (such as 15°C) can shorten the dormancy period of the seven-flower to a certain extent, but the germination rate, germination potential and germination index are significantly lower; while a higher temperature (such as 30°C) is not conducive to the germination of the seven-flower seeds, and even has a great inhibitory effect on the germination of the seven-flower seeds. The seven-flower seeds cultivated in the experiment basically have no signs of germination; the most suitable temperature condition for the germination of the seven-flower seeds should be when the temperature is set to 25°C, and its germination rate, germination potential and germination index are the highest (as shown in Table 1).

[0044] Table 1 Effects of different temperatures on the germination of seven-flowered lilies seeds

[0045] 2. Effects of various types of hormones with different concentration gradients on the germination of seven-flowered flowers During the experiment, the method of Example 1 was used to treat the seven-son flower seeds with different hormones and hormones of different concentration gradients during the hormone treatment before germination. It was found that although a higher concentration of a single hormone can shorten the dormancy period of the seven-son flower seeds to a certain extent, thereby achieving the purpose of promoting the germination of the seven-son flower seeds, for the germination rate, germination potential and germination index of the seven-son flower seeds, a single hormone of different concentration gradients will have a certain degree of inhibitory effect on the germination rate, germination potential and germination index of the seven-son flower seeds.

[0046] (1) Effects of different concentration gradients of GA3 on the germination characteristics of seven-year-old seeds The effects of different concentration gradients of GA3 on the germination characteristics of seven-year-old seeds are shown in Table 2.

[0047] Table 2 Effects of different concentration gradients of GA3 on seed germination of Hesperus rotundus

[0048] (2) Effects of different IAA concentration gradients on the germination characteristics of Hesperus sphenanthera seeds The effects of different concentration gradients of IAA on the germination characteristics of seven-year-old seeds are shown in Table 3.

[0049] Table 3 Effects of different concentration gradients of IAA--auxin on the germination of Heptaphyllum seeds

[0050] The above experiments show that higher or lower concentrations of hormones will obviously have a certain degree of inhibitory effect on the germination of seven-son flower seeds.

[0051] (3) Effects of mixed hormones at different concentration gradients on the germination characteristics of Hesperus rotundus seeds Under the same conditions, there were significant differences in the germination rate, germination potential and germination index of seven-single floribunda seeds soaked in different concentration gradient mixed hormones. High concentration of mixed hormones could not increase the germination rate of seven-single floribunda seeds, and had a certain inhibitory effect. However, lower concentration of mixed hormones had a certain promoting effect on the germination rate, germination potential and germination index of seven-single floribunda, and could make the seven-single floribunda seeds germinate faster and more uniformly, and could effectively improve the vitality of seeds (as shown in Table 4).

[0052] Table 4 Effects of different concentration gradient mixed hormones on the germination characteristics of seven-flowered lily seeds

[0053] The above describes the embodiments of the present invention in detail in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. For ordinary technicians in this field, several modifications and improvements can be made without departing from the present invention, and these should also be regarded as belonging to the protection scope of the present invention.

Claims

1. A method for propagating seven-flowered lily seeds, characterized in that: The breeding method comprises the following steps: (1) Pruning and thinning flowers: In July, in the distribution area of ​​the seven-son flower, prune the branches of the seven-son flower in the early flowering period, remove the tops, and remove some of the excess flowers; (2) Fertilization during flowering period: During the flowering period of Heptaphyllum, apply more phosphorus fertilizer to promote embryo development and maturity; (3) Seed collection: Collect plump seeds with purple or dark red calyx from late October to early November and set aside; (4) Seed selection: Select fresh seeds with a single seed weight of 2 to 4 g, a length of 10 to 13 cm, and a diameter of 2 to 3 cm, soak them in a sterilizing solution for 2 to 3 hours, then wash them with sterile water, and air-dry them indoors for 30 days to keep the moisture content of the seeds at 7% to 10%; (5) Low temperature treatment: The naturally air-dried seven-son flower seeds are placed in a seed box for storage, and the seven-son flower seeds are treated alternately at different temperatures. First, the seven-son flower seeds are placed at a temperature of 25°C for 40 days, and then the seven-son flower seeds are placed at a temperature of 4°C for 20 days. The temperature treatment is alternated two to four times; (6) Selection and sterilization of substrate: Select a mixture of peat and coconut bran, with the mass ratio of peat and coconut bran set to 1:4-5. Select river sand, with the mass ratio of river sand to the mixed soil set to 1:1-2. Sterilize the above substrate for seed germination at high temperature, with the sterilization temperature set to 120-130°C and the sterilization time set to 2-3h. After sterilization, spread a 10-13cm thick mixture of peat and coconut bran on the bottom of the germination container, and cover the top of the mixed soil with 7-9cm of river sand. (7) Hormone treatment before germination: Place the seeds of Hesperus rotundus that have been treated with low temperature in a rooting solution and soak for 36 to 48 hours. The rooting solution consists of GA3, 6-BA and NAA. The concentration of GA3 is 1-50 mg / L, the concentration of 6-BA is 1-4 mg / L, and the concentration of NAA is 0.2-0.4 mg / L. The mass ratio of GA3:6-BA:NAA is 200-250:5-10:

1. (8) Seed disinfection: Take out the seeds that have been treated with pre-germination hormones and disinfect them with 70% alcohol; (9) Seed germination: Place the seeds disinfected with alcohol in a germination container containing a sterilized substrate. The seeds are placed on the surface of a mixture of peat soil and coconut bran. Cover the top of the mixed soil with wet river sand. Place the germination container in an artificial climate box with constant temperature and light for cultivation. The temperature of the artificial climate box is set at 20-25°C, with alternating light of 1200 Lux / total darkness for 14h / 10h during day and night, and humidity of 65-85%. Keep the surface of the river sand moist. After 136 days, the seeds take root and germinate, the cotyledons unfold, and then grow true leaves. (10) Management during the seedling stage: After the seven-son flower grows three true leaves, the temperature of the artificial climate box is set to 24-27°C, the light duration is set to 12-14 hours / day, and the light intensity is set to 500-1000 Lux, of which the light intensity is set to 1000 Lux from 7:00 to 10:00 in the morning, 500 Lux from 10:00 to 14:00, and 8000 Lux from 14:00 to 19:

00. The relative humidity is set to 75-85%, and water is applied every other day. (11) Seedling transplantation: After the management during the seedling stage, when the seven-song flower grows into a seedling with a height of 20 to 40 cm, it is transplanted from the artificial climate chamber to a suitable natural environment or potted.

2. The method for propagating seven-son flower seeds according to claim 1, characterized in that: During the low temperature treatment in step (5), the temperature alternation treatment is set to be three times.

3. The method for propagating seven-son flower seeds according to claim 1, characterized in that: In the hormone treatment before germination in step (7), the rooting solution is composed of GA3, 6-BA and NAA, the concentration of GA3 is 10-20 mg / L, the concentration of 6-BA is 2-3 mg / L, and the concentration of NAA is 0.2-0.3 mg / L; the mass ratio of GA3:6-BA:NAA is 220~230:7~8:

1.

4. The method for propagating seven-son flower seeds according to claim 1, characterized in that: During the germination of the seeds in step (9), the temperature of the artificial climate box is set to 25°C.

5. The method for propagating seven-son flower seeds according to claim 1, characterized in that: During the germination of the seeds in step (9), the humidity is set to 75-80%.

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