Cultivation method for shortening cultivation period of trachinotus ovatus

By segmenting the breeding area and spraying the compound bacterial solution, combining different nutrients and light management, the stress response and disease problems caused by environmental differences in the breeding process of golden pomfret are solved, rapid growth and efficient breeding are achieved, and the breeding cycle is shortened. Improved the quality of fish.

CN119111432AActive Publication Date: 2024-12-13HAINAN CHENHAI AQUATIC CO LTD

Patent Information

Application Number
CN202411587776.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-08
Publication Date
2024-12-13
Estimated Expiration
2044-11-08

AI Technical Summary

Technical Problem

Stress response and disease problems caused by differences in environmental conditions during the breeding of golden pomfrets affect the growth cycle and economic benefits.

Method used

Use the split breeding area and spray the compound bacterial solution, and distribute different nutrients according to the specifications of the fry to control the water temperature and light intensity. Through intermittent irradiation and feed management of different particle sizes, the rapid growth and immunity of golden pomfret are promoted. .

Benefits of technology

The breeding cycle of golden pomfret has been shortened, immunity and meat quality have been improved, disease and mortality have been reduced, and breeding benefits have been improved.

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Abstract

The invention provides a culture method for shortening the culture cycle of trachinotus ovatus, which specifically comprises the following steps: (1) setting and dividing different culture areas, and spraying compound bacteria liquid in each culture area before fry casting; (2) fry putting: healthy trachinotus ovatus fries are screened and put into each culture area; (3) feed feeding: feeding different nutrient materials in different culture areas; (4) cultivation management; (5) daily management: arranging a light source lamp above the breeding area. By means of the breeding method, the breeding period of the trachinotus ovatus is obviously shortened, the situation that the breeding environment is converted in the breeding process of the trachinotus ovatus is avoided, and the situation that the trachinotus ovatus generates stress reaction or is infected with various diseases, and consequently the breeding cost is increased is avoided. The golden pomfret obtained through the breeding method is high in growth speed, the immunity is improved, the morbidity and the mortality rate are reduced in the breeding process, and the flavor quality of fish products is improved.
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Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a breeding method for shortening the breeding cycle of golden pomfret. Background Art

[0002] The scientific name of golden pomfret is ovate pomfret, and its local names are yellow wax pomfret and golden pomfret. Golden pomfret is rich in nutrition, high in protein, and rich in unsaturated fatty acids, which can lower cholesterol. Golden pomfret is a warm-water pelagic fish with a large body, large appetite, fast digestion, and a fierce appetite for food. It grows rapidly. From seedlings, it can reach the market specification of 400-600g in 5-6 months. Farmers can choose to increase the feeding amount in June, July, and August when golden pomfret grows fastest according to the characteristics of its fast growth rate, so as to promote its rapid growth, reach the market specification as soon as possible, compress its growth cycle, and reduce risks. However, if the feeding amount is increased only during the breeding process in order to maximize the growth rate, it often leads to enteritis and death of golden pomfret, affecting the normal growth of golden pomfret, and at the same time affecting immunity and meat quality, thereby affecting the economic benefits of breeding.

[0003] During the breeding process, golden pomfret at different stages needs to be raised in different areas, and the environmental conditions vary greatly. This may cause the golden pomfret to have a stress response or be infected with various diseases, seriously affecting the economic benefits of breeding and increasing breeding costs. Summary of the invention

[0004] In view of this, the present invention proposes a breeding method for shortening the breeding cycle of golden pomfret to solve the above technical problems, so that the breeding cycle of golden pomfret is shortened without affecting its immunity and meat quality.

[0005] The technical solution of the present invention is achieved in this way: A breeding method for shortening the breeding cycle of golden pomfret, the specific steps comprising: (1) Setting up the breeding area: The breeding area is divided into breeding areas A, B, C and D, and seawater is injected. The water temperature is controlled at 22-25°C. Before stocking, the composite bacterial solution is sprayed in each breeding area; (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 100-200 fry / m 3 ; Fry weighing 11-30g are placed in breeding area B at a density of 80-100 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 50-80 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 7:00-8:00 every morning, and feeding again every 2-3 hours. The amount of each feeding is 1%-2% of the weight of the fry; Feed the nutrient feed B in the breeding area B, starting at 7:00-8:00 every morning, and feeding again every 4-5 hours, with each feeding amount being 3%-4% of the weight of the fry; Feed the nutrient C in the breeding area C, starting at 7:00-8:00 every morning, and feeding again every 6-7 hours. The feeding amount each time is 5%-7% of the weight of the fry; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density in breeding area D is 30-50 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 7:00-8:00 every morning, and feeding again every 10-12 hours, with each feeding amount being 8%-10% of the weight of the fry; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 800-1200 Lux for 1-3 minutes and an interval of 25-30 minutes. In breeding area B, the light intensity is 1500-1800 Lux, the light is irradiated for 4-6 minutes, and the interval is 15-20 minutes. In breeding area C, the light intensity is 1900-2100 Lux, the light duration is 8-10 minutes, and the interval is 5-10 minutes. The breeding area D is intermittently irradiated with a light intensity of 2200-2500 Lux, irradiation for 10-12 hours, and intermittent for 10-12 hours.

[0006] Furthermore, in step (1), the composite bacterial liquid is a liquid of Rhodopseudomonas palustris, a liquid of Armillaria pseudomellea, and a liquid of Pediococcus pentosaceus in a mass ratio of 3-5:2-4:1; and the specifications of the culture areas A, B, C, and D are all 5.5 m×5.5 m×5.5 m.

[0007] Furthermore, in step (3), the nutrient material A comprises the following raw materials in parts by weight: 30-40 parts of fish meal, 5-8 parts of corn pollen, 3-5 parts of pomegranate peel polysaccharide, 1-3 parts of isomaltooligosaccharide, 1-2 parts of galacto-oligosaccharide, 2-4 parts of theanine, 0.8-1.2 parts of lactobacillus freeze-dried powder, 3-5 parts of pomegranate peel extract, and 5-7 parts of vegetable blending oil.

[0008] Furthermore, the preparation method of the nutrient A includes: evenly mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:3-5:2-4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

[0009] Furthermore, in step (3), the nutrient B is prepared by adding nutrient b to nutrient A; The nutrient material B comprises, by weight: 0.7-0.9 parts of lactulose, 1-1.5 parts of soybean oligosaccharides, 1-3 parts of bacillus freeze-dried powder, 4-7 parts of bayberry fruit anthocyanin extract, 1-3 parts of Panax notoginseng polysaccharide, and 0.5-1 parts of casein phosphopeptide.

[0010] Furthermore, the preparation method of the nutrient material B includes: uniformly mixing lactulose, soybean oligosaccharides, Bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, drying and screening to obtain particles with a mass ratio of 1:2-4:1-3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

[0011] Furthermore, in step (3), the nutrient C is added to the nutrient B: The nutrient material C comprises, by weight: 0.2-0.5 parts of vitamin C, 0.4-0.8 parts of palatinose, 2-4 parts of Polygonum hydropiper extract, 1-3 parts of Acanthopanax senticosus polysaccharide, and 0.8-1.2 parts of freeze-dried yeast powder.

[0012] Furthermore, the preparation method of the nutrient material C includes: uniformly mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 5-7:1:2-4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

[0013] Furthermore, in step (4), the nutrient D is prepared by adding nutrient d to nutrient C: The nutrient material d comprises, by weight: 5-10 parts of Artemia, 1-3 parts of coupled fructose, 8-10 parts of vegetable powder, 0.6-0.8 parts of bio-peptide fermentation liquid, 0.6-0.8 parts of gentian oligosaccharide, 3-5 parts of sea grape extract, and 1-2 parts of Pleurotus eryngii polysaccharide.

[0014] Furthermore, the preparation method of the nutrient material d includes: evenly mixing Artemia, coupled fructose, vegetable powder, bio-peptide fermentation broth, gentian oligosaccharides, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, drying and screening to obtain particles with a mass ratio of 4-6:1-3:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The breeding method of the present invention is scientific and reasonable. By dividing different breeding areas for management, golden pomfret is put into different breeding areas according to the specifications of golden pomfret, and different nutrients are matched to promote the golden pomfret to absorb and utilize the nutrient feed well. The nutrients can float on the water surface and sink to the bottom of the water by setting different particle sizes, thereby reducing the feeding competition of golden pomfret and achieving reasonable distribution. Before stocking, a composite bacterial liquid composed of a liquid of Rhodopseudomonas palustris, a liquid of Armillaria pseudomellea, and a liquid of Pediococcus pentosaceus is sprayed in the breeding area. The composite bacterial liquid can not only purify the water quality, but also regulate the intestinal flora of golden pomfret, accelerate the absorption and utilization of nutrients, promote digestion, and achieve rapid growth, thereby improving the immunity of golden pomfret and reducing diseases in the breeding process of golden pomfret.

[0016] 2. The breeding method of the present invention significantly shortens the breeding period of golden pomfret from the traditional 5-6 months to 3 months. In addition, the breeding process avoids the stress response or infection of various diseases caused by the conversion of the breeding environment during the breeding process of golden pomfret, thereby increasing the breeding cost. The breeding method of the present invention makes the golden pomfret grow faster, improve immunity, reduce morbidity and mortality during the breeding process, and improve the flavor quality of the fish. DETAILED DESCRIPTION

[0017] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0018] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0019] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0020] The vegetable blended oil of the present invention was purchased from Jiangxi Lvyuan Oil Industry Co., Ltd.

[0021] The biological peptide fermentation liquid of the invention is obtained by mixing and fermenting black soldier fly larvae and papain.

[0022] Example 1 The breeding method of shortening the breeding period of golden pomfret of the present embodiment comprises the following specific steps: (1) Setting up the culture area: The culture area is divided into culture areas A, B, C and D. The size of each area is 5.5m×5.5m×5.5m. Seawater is injected and the water temperature is controlled at 23±1℃. Before stocking, a composite bacterial solution is sprayed in each culture area. The composite bacterial solution is a solution of Rhodopseudomonas palustris, a solution of Armillaria pseudomellea and a solution of Pediococcus pentosaceus in a mass ratio of 3-5:2-4:1. (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 150 fry / m 3 ; Fry weighing 11-30g are placed in breeding area B at a density of 90 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 70 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 7:30 a.m. every day, and feeding again every 2 hours. The amount of each feeding is 1% of the weight of the fry; The nutrient material A comprises the following raw materials in parts by weight: 35 parts of fish meal, 7 parts of corn pollen, 4 parts of pomegranate peel polysaccharide, 2 parts of isomaltooligosaccharide, 1.5 parts of galacto-oligosaccharide, 3 parts of theanine, 1 part of lactobacillus freeze-dried powder, 4 parts of pomegranate peel extract, and 6 parts of vegetable blending oil; The preparation method of nutrient material A comprises: mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil uniformly, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:4:3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feeding nutrient B in breeding area B starts at 7:30 a.m. every day and the next feeding is done every 4 hours. The feeding amount each time is 3% of the weight of the fry. The nutrient B is prepared by adding nutrient b to nutrient A, and the nutrient b comprises, by weight: 0.8 parts of lactulose, 1.3 parts of soybean oligosaccharides, 2 parts of bacillus freeze-dried powder, 5 parts of bayberry fruit anthocyanin extract, 2 parts of Panax japonicus polysaccharide, and 0.8 parts of casein phosphopeptide; The preparation method of nutrient material B comprises: uniformly mixing lactulose, soybean oligosaccharides, bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 1:3:2 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feed the nutrient C in the culture area C, starting at 7:30 a.m. every day, and feeding every 6 hours. The feeding amount each time is 6% of the weight of the fry; The nutrient C is prepared by adding nutrient C to nutrient B, and the nutrient C includes, by weight: 0.3 parts of vitamin C, 0.6 parts of palatinose, 3 parts of Polygonum hydropiper extract, 2 parts of Acanthopanax senticosus polysaccharide, and 1 part of freeze-dried yeast powder; The preparation method of nutrient material C comprises: mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder evenly, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 6:1:3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density of breeding area D is 40 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 7:30 a.m. every day, and feeding again every 11 hours, with the feeding amount each time being 9% of the weight of the fry; The nutrient D is nutrient C plus nutrient D, and the nutrient D includes, by weight: 8 parts of Artemia, 2 parts of coupled fructose, 9 parts of vegetable powder, 0.7 parts of biopeptide fermentation liquid, 0.7 parts of gentian oligosaccharide, 4 parts of sea grape extract, and 1.5 parts of Pleurotus eryngii polysaccharide; The preparation method of nutrient material d comprises: uniformly mixing Artemia, coupled fructose, vegetable powder, biopeptide fermentation liquid, oligosaccharide of gentian, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 5:2:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 1000 Lux for 2 minutes and an interval of 25 minutes. In breeding area B, intermittent irradiation was performed with a light intensity of 1700 Lux for 5 min and a rest period of 15 min. In the breeding area C, the light intensity was 2000 Lux, the irradiation time was 9 min, and the rest time was 5 min. The culture area D is intermittently irradiated with a light intensity of 2400 Lux, irradiation for 11 hours and rest for 11 hours.

[0023] Example 2 The breeding method of shortening the breeding period of golden pomfret of the present embodiment comprises the following specific steps: (1) Culture area setting: The culture area is divided into culture areas A, B, C and D. The size of each area is 5.5m×5.5m×5.5m. Seawater is injected and the water temperature is controlled at 23±1℃. Before stocking, a composite bacterial solution is sprayed in each culture area. The composite bacterial solution is a solution of Rhodopseudomonas palustris, a solution of Armillaria pseudomellea and a solution of Pediococcus pentosaceus in a mass ratio of 3:2:1. (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 100 fry / m 3 ; Fry weighing 11-30g are placed in breeding area B at a density of 80 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 50 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 7:00 a.m. every day, and feeding every 3 hours. The feeding amount each time is 1%-1% of the weight of the fry; The nutrient material A comprises the following raw materials in parts by weight: 30 parts of fish meal, 5 parts of corn pollen, 3 parts of pomegranate peel polysaccharide, 1 part of isomaltooligosaccharide, 1 part of galacto-oligosaccharide, 2 parts of theanine, 0.8 parts of lactobacillus freeze-dried powder, 3 parts of pomegranate peel extract, and 5 parts of vegetable blending oil; The preparation method of nutrient material A comprises: mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil uniformly, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:3:2 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feed the nutrient feed B in the breeding area B, starting at 7:00 a.m. every day, and feeding again every 5 hours. The feeding amount each time is 3% of the weight of the fry; The nutrient B is prepared by adding nutrient b to nutrient A, and the nutrient b comprises, by weight: 0.7 parts of lactulose, 1 part of soybean oligosaccharide, 1 part of bacillus freeze-dried powder, 4 parts of bayberry fruit anthocyanin extract, 1 part of Panax notoginseng polysaccharide, and 0.5 parts of casein phosphopeptide; The preparation method of nutrient material B comprises: uniformly mixing lactulose, soybean oligosaccharides, bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 1:2:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feed the nutrient C in the breeding area C, starting at 7:00 a.m. every day, and feeding again every 7 hours. The feeding amount each time is 5% of the weight of the fry; The nutrient C is prepared by adding nutrient C to nutrient B, and the nutrient C includes, by weight: 0.2 parts of vitamin C, 0.4 parts of palatinose, 2 parts of Polygonum hydropiper extract, 1 part of Acanthopanax senticosus polysaccharide, and 0.8 parts of freeze-dried yeast powder; The preparation method of nutrient material C comprises: mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder evenly, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 5:1:2 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density of breeding area D is 30 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 7:00 a.m. every day, and feeding again every 10 hours, with the feeding amount each time being 8% of the weight of the fry; The nutrient D is nutrient D added to nutrient C, and the nutrient D includes, by weight: 5 parts of Artemia, 1 part of coupled fructose, 8 parts of vegetable powder, 0.6 parts of biopeptide fermentation liquid, 0.6 parts of gentian oligosaccharide, 3 parts of sea grape extract, and 1 part of Pleurotus eryngii polysaccharide; The preparation method of nutrient material d comprises: uniformly mixing Artemia, coupled fructose, vegetable powder, biopeptide fermentation liquid, oligosaccharide of gentian, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 4:1:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 800 Lux for 1 minute and a rest period of 30 minutes. In breeding area B, intermittent irradiation was performed with a light intensity of 1500 Lux for 4 minutes and a rest period of 20 minutes. In breeding area C, intermittent irradiation was performed with a light intensity of 1900 Lux for 8 min and a rest period of 10 min. The culture area D is intermittently irradiated with a light intensity of 2200 Lux for 10 hours and a rest period of 10 hours.

[0024] Example 3 The breeding method of shortening the breeding period of golden pomfret of the present embodiment comprises the following specific steps: (1) Setting up the culture area: The culture area is divided into culture areas A, B, C and D. The size of each area is 5.5m×5.5m×5.5m. Seawater is injected and the water temperature is controlled at 23±1℃. Before stocking, a composite bacterial solution is sprayed in each culture area. The composite bacterial solution is a solution of Rhodopseudomonas palustris, a solution of Armillaria pseudomellea and a solution of Pediococcus pentosaceus in a mass ratio of 5:4:1. (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 200 fry / m 3 ; Fry weighing 11-30g are placed in culture area B at a density of 100 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 80 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 8:00 a.m. every day, and feeding every 3 hours. The amount of each feeding is 2% of the weight of the fry; The nutrient material A comprises the following raw materials in parts by weight: 40 parts of fish meal, 8 parts of corn pollen, 5 parts of pomegranate peel polysaccharide, 3 parts of isomaltooligosaccharide, 2 parts of galacto-oligosaccharide, 4 parts of theanine, 1.2 parts of lactobacillus freeze-dried powder, 5 parts of pomegranate peel extract, and 7 parts of vegetable blending oil; The preparation method of nutrient material A comprises: mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil uniformly, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:5:4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feeding nutrient B in breeding area B starts at 8:00 a.m. every day and the next feeding is done every 5 hours. The feeding amount each time is 4% of the weight of the fry. The nutrient material B is prepared by adding nutrient material B to nutrient material A, and the nutrient material B comprises, by weight: 0.9 parts of lactulose, 1.5 parts of soybean oligosaccharides, 3 parts of bacillus freeze-dried powder, 7 parts of bayberry fruit anthocyanin extract, 3 parts of Panax japonicus polysaccharide, and 1 part of casein phosphopeptide; The preparation method of nutrient material B comprises: uniformly mixing lactulose, soybean oligosaccharides, bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 1:4:3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; Feed the nutrient C in the breeding area C, starting at 8:00 a.m. every day, and feeding again every 7 hours. The feeding amount each time is 7% of the weight of the fry; The nutrient C is prepared by adding nutrient C to nutrient B, and the nutrient C includes, by weight: 0.5 parts of vitamin C, 0.8 parts of palatinose, 4 parts of Polygonum hydropiper extract, 3 parts of Acanthopanax senticosus polysaccharide, and 1.2 parts of freeze-dried yeast powder; The preparation method of nutrient material C comprises: mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder evenly, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 7:1:4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density of breeding area D is 50 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 8:00 a.m. every day, and feeding again every 12 hours, with the feeding amount each time being 10% of the weight of the fry; The nutrient material D is nutrient material C plus nutrient material D, and the nutrient material D includes, by weight: 10 parts of Artemia, 3 parts of coupled fructose, 10 parts of vegetable powder, 0.8 parts of biopeptide fermentation liquid, 0.8 parts of gentian oligosaccharide, 5 parts of sea grape extract, and 2 parts of Pleurotus eryngii polysaccharide; The preparation method of nutrient material d comprises: uniformly mixing Artemia, coupled fructose, vegetable powder, biopeptide fermentation liquid, oligosaccharide of gentian, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 6:3:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 1200 Lux for 3 minutes and a rest period of 30 minutes. In breeding area B, intermittent irradiation was performed with a light intensity of 1800 Lux for 6 min and a rest period of 20 min. In the breeding area C, the light intensity is 2100 Lux, the irradiation time is 10 min, and the rest time is 10 min. The culture area D is intermittently irradiated with a light intensity of 2500 Lux, irradiation for 12 hours and rest for 12 hours.

[0025] Comparative Example 1 The difference from Example 1 is that the breeding area is not divided, and the rest is the same as Example 1.

[0026] The breeding method for shortening the breeding cycle of golden pomfret in this comparative example comprises the following specific steps: (1) Culture area setting: The culture area is 5.5m×5.5m×5.5m in size and injected with seawater. The water temperature is controlled at 23±1℃. Before stocking, the culture area is sprayed with a composite bacterial solution, which is a solution of Rhodopseudomonas palustris, Armillaria pseudomellea and Pediococcus pentosaceus in a mass ratio of 3-5:2-4:1. (2) Fish fry release: Select healthy golden pomfret fry weighing 3-10g and release them into the breeding area at a density of 150 fry / m 3 ; (3) Feeding: Feed nutrient feed A, starting at 7:30 a.m. every day, and feeding every 2 hours. The feeding amount each time is 1% of the weight of the fry. When the weight of the golden pomfret in the culture area reaches more than 500 g, it is immediately captured and salvaged; The nutrient material A comprises the following raw materials in parts by weight: 35 parts of fish meal, 7 parts of corn pollen, 4 parts of pomegranate peel polysaccharide, 2 parts of isomaltooligosaccharide, 1.5 parts of galacto-oligosaccharide, 3 parts of theanine, 1 part of lactobacillus freeze-dried powder, 4 parts of pomegranate peel extract, and 6 parts of vegetable blending oil; The preparation method of nutrient material A comprises: mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil uniformly, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:4:3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm; (4) Daily management: A light source is installed above the breeding area, which irradiates intermittently with a light intensity of 1000 Lux, irradiating for 2 minutes and resting for 25 minutes.

[0027] Comparative Example 2 The difference from Example 1 is that the composite bacterial liquid is not sprayed, and the other steps are the same as Example 1.

[0028] That is, step (1) in the aquaculture method for shortening the aquaculture cycle of the present comparative example is: aquaculture area setting: the aquaculture area is divided into aquaculture areas A, B, C and D, each area has a size of 5.5m×5.5m×5.5m, seawater is injected, and the water temperature is controlled at 23±1°C.

[0029] Comparative Example 3 The difference from Example 1 is that the nutrient material A lacks pomegranate peel extract and pomegranate peel polysaccharide, and the rest is the same as Example 1.

[0030] Comparative Example 4 The difference from Example 1 is that nutrient material b lacks bayberry fruit anthocyanin extract and Panax notoginseng polysaccharide, and the rest is consistent with Example 1.

[0031] Comparative Example 5 The difference from Example 1 is that the nutrient material c lacks palatinose and Acanthopanax senticosus polysaccharide, and the rest is consistent with Example 1.

[0032] Comparative Example 6 The difference from Example 1 is that the nutrient material d lacks bio-peptide fermentation liquid, sea grape extract and Pleurotus eryngii polysaccharide, and the rest is consistent with Example 1.

[0033] Comparative Example 7 The difference from Example 1 is that the particle size of each nutrient particle is not limited, and the rest is the same as Example 1.

[0034] That is, the breeding method for shortening the breeding cycle of golden pomfret of this comparative example comprises the following specific steps: (1) Setting up the culture area: The culture area is divided into culture areas A, B, C and D. The size of each area is 5.5m×5.5m×5.5m. Seawater is injected and the water temperature is controlled at 23±1℃. Before stocking, a composite bacterial solution is sprayed in each culture area. The composite bacterial solution is a solution of Rhodopseudomonas palustris, a solution of Armillaria pseudomellea and a solution of Pediococcus pentosaceus in a mass ratio of 3-5:2-4:1. (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 150 fry / m 3 ; Fry weighing 11-30g are placed in breeding area B at a density of 90 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 70 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 7:30 a.m. every day, and feeding again every 2 hours. The amount of each feeding is 1% of the weight of the fry; The nutrient material A comprises the following raw materials in parts by weight: 35 parts of fish meal, 7 parts of corn pollen, 4 parts of pomegranate peel polysaccharide, 2 parts of isomaltooligosaccharide, 1.5 parts of galacto-oligosaccharide, 3 parts of theanine, 1 part of lactobacillus freeze-dried powder, 4 parts of pomegranate peel extract, and 6 parts of vegetable blending oil; The preparation method of nutrient material A comprises: mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil uniformly, puffing and granulating, and drying; Feeding nutrient B in breeding area B starts at 7:30 a.m. every day and the next feeding is done every 4 hours. The feeding amount each time is 3% of the weight of the fry. The nutrient B is prepared by adding nutrient b to nutrient A, and the nutrient b comprises, by weight: 0.8 parts of lactulose, 1.3 parts of soybean oligosaccharides, 2 parts of bacillus freeze-dried powder, 5 parts of bayberry fruit anthocyanin extract, 2 parts of Panax japonicus polysaccharide, and 0.8 parts of casein phosphopeptide; The preparation method of nutrient material B comprises: uniformly mixing lactulose, soybean oligosaccharide, bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, and drying; Feed the nutrient C in the culture area C, starting at 7:30 a.m. every day, and feeding every 6 hours. The feeding amount each time is 6% of the weight of the fry; The nutrient C is prepared by adding nutrient C to nutrient B, and the nutrient C includes, by weight: 0.3 parts of vitamin C, 0.6 parts of palatinose, 3 parts of Polygonum hydropiper extract, 2 parts of Acanthopanax senticosus polysaccharide, and 1 part of freeze-dried yeast powder; The preparation method of nutrient material C comprises: mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder uniformly, puffing and granulating, and drying; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density of breeding area D is 40 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 7:30 a.m. every day, and feeding again every 11 hours, with the feeding amount each time being 9% of the weight of the fry; The nutrient D is nutrient C plus nutrient D, and the nutrient D includes, by weight: 8 parts of Artemia, 2 parts of coupled fructose, 9 parts of vegetable powder, 0.7 parts of biopeptide fermentation liquid, 0.7 parts of gentian oligosaccharide, 4 parts of sea grape extract, and 1.5 parts of Pleurotus eryngii polysaccharide; The preparation method of the nutrient material d comprises: uniformly mixing Artemia, coupled fructose, vegetable powder, biopeptide fermentation liquid, oligosaccharides of gentian, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, and drying; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 1000 Lux for 2 minutes and an interval of 25 minutes. In breeding area B, intermittent irradiation was performed with a light intensity of 1700 Lux for 5 min and a rest period of 15 min. In the breeding area C, the light intensity was 2000 Lux, the irradiation time was 9 min, and the rest time was 5 min. The culture area D is intermittently irradiated with a light intensity of 2400 Lux, irradiation for 11 hours and rest for 11 hours.

[0035] Test example The golden pomfret was cultured according to the culture methods of Examples 1-3 and Comparative Examples 1-7, and the survival rate, feed coefficient and disease status of the caught golden pomfret were counted, and the crude protein content of the golden pomfret was determined; Survival rate (%) = number of fish caught alive / total number of fish released × 100% Feed coefficient = total weight of feed consumed / total weight gain of fish Enteritis morbidity rate (%) = number of enteritis-affected fish / number of fry released × 100% Ichthyophthirius morbidity rate (%) = number of Ichthyophthirius morbidity / number of fry released × 100% Among them, the number of golden pomfret suffering from enteritis is the number of golden pomfret suffering from enteritis when white feces are found in the autopsy of dead golden pomfret; the symptoms of Ichthyophthirius affliciensis are: increased mucus in the golden pomfret's gills, small needle-shaped white spots scattered on the body surface, and the body color changes from smooth yellow to rough black and white skin.

[0036] Crude protein content: Kjeldahl method in "GB 5009.5-2016 National Food Safety Standard Determination of Protein in Food".

[0037] The results are shown in Table 1.

[0038] Table 1 As can be seen from Table 1, compared with Comparative Examples 1-7, the golden pomfret obtained by the breeding method of Examples 1-3 of the present invention has a high survival rate, can quickly digest feed, has a high utilization rate of feed, improves the immunity of golden pomfret, reduces diseases during the breeding process of golden pomfret, and improves the flavor quality of fish products. In addition, the breeding method of Examples 1-3 significantly shortens the breeding period of golden pomfret.

[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A breeding method for shortening the breeding cycle of golden pomfret, characterized in that: The specific steps include: (1) Setting up the breeding area: The breeding area is divided into breeding areas A, B, C and D, and seawater is injected. The water temperature is controlled at 22-25°C. Before stocking, the composite bacterial solution is sprayed in each breeding area; (2) Fish fry release: Select healthy golden pomfret fry and release them into the breeding area. Fry weighing 3-10g are released into breeding area A at a density of 100-200 fry / m 3 ; Fry weighing 11-30g are placed in breeding area B at a density of 80-100 / m 3 ; Fry weighing 31-50g are placed in culture area C at a density of 50-80 / m 3 ; (3) Feeding: Feed nutrient feed A in breeding area A, starting at 7:00-8:00 every morning, and feeding again every 2-3 hours. The amount of each feeding is 1%-2% of the weight of the fry; Feed the nutrient feed B in the breeding area B, starting at 7:00-8:00 every morning, and feeding again every 4-5 hours, with each feeding amount being 3%-4% of the weight of the fry; Feed the nutrient C in the breeding area C, starting at 7:00-8:00 every morning, and feeding again every 6-7 hours. The feeding amount each time is 5%-7% of the weight of the fry; (4) Cultivation and management: Based on the growth of the fry, when the fry in culture area A weighs more than 10 g, they will be immediately removed and placed in culture area B for further breeding; When the fish in breeding area B are larger than 30g, they are immediately removed and placed in breeding area C for further breeding; When the fish in breeding area C are larger than 50g, they are immediately removed and placed in breeding area D for feeding. The density in breeding area D is 30-50 fish / m 3 Feed the nutrient material D in the breeding area D, starting at 7:00-8:00 every morning, and feeding again every 10-12 hours, with each feeding amount being 8%-10% of the weight of the fry; When the weight of golden pomfret in breeding area D reaches more than 500g, it is immediately captured and salvaged; (5) Daily management: A light source is installed above the breeding area. Breeding area A is irradiated intermittently with a light intensity of 800-1200 Lux for 1-3 minutes and an interval of 25-30 minutes. In breeding area B, the light intensity is 1500-1800 Lux, the light is irradiated for 4-6 minutes, and the interval is 15-20 minutes. In breeding area C, the light intensity is 1900-2100 Lux, the light duration is 8-10 minutes, and the interval is 5-10 minutes. The breeding area D is intermittently irradiated with a light intensity of 2200-2500 Lux, irradiation for 10-12 hours, and intermittent for 10-12 hours.

2. A method for shortening the breeding cycle of golden pomfret as claimed in claim 1, characterized in that: In step (1), the composite bacterial liquid is a liquid of Rhodopseudomonas palustris, a liquid of Armillaria pseudomellea and a liquid of Pediococcus pentosaceus in a mass ratio of 3-5:2-4:1; and the specifications of the culture areas A, B, C and D are all 5.5m×5.5m×5.5m.

3. A method for shortening the breeding cycle of golden pomfret as claimed in claim 1, characterized in that: In step (3), the nutrient material A comprises the following raw materials in parts by weight: 30-40 parts of fish meal, 5-8 parts of corn pollen, 3-5 parts of pomegranate peel polysaccharide, 1-3 parts of isomaltooligosaccharide, 1-2 parts of galacto-oligosaccharide, 2-4 parts of theanine, 0.8-1.2 parts of lactobacillus freeze-dried powder, 3-5 parts of pomegranate peel extract, and 5-7 parts of vegetable blending oil.

4. A method for shortening the breeding cycle of golden pomfret as claimed in claim 3, characterized in that: The preparation method of the nutrient material A comprises: uniformly mixing fish meal, corn pollen, pomegranate peel polysaccharide, isomaltooligosaccharide, galacto-oligosaccharide, theanine, lactobacillus freeze-dried powder, pomegranate peel extract and vegetable blending oil, puffing and granulating, drying and grinding, and screening out particles with a mass ratio of 1:3-5:2-4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

5. A method for shortening the breeding cycle of golden pomfret as claimed in claim 1, characterized in that: In step (3), the nutrient B is prepared by adding nutrient b to nutrient A; The nutrient material B comprises, by weight: 0.7-0.9 parts of lactulose, 1-1.5 parts of soybean oligosaccharides, 1-3 parts of bacillus freeze-dried powder, 4-7 parts of bayberry fruit anthocyanin extract, 1-3 parts of Panax notoginseng polysaccharide, and 0.5-1 parts of casein phosphopeptide.

6. A method for shortening the breeding cycle of golden pomfret as claimed in claim 5, characterized in that: The preparation method of the nutrient material B comprises: uniformly mixing lactulose, soybean oligosaccharides, bacillus freeze-dried powder, bayberry fruit anthocyanin extract, Panax notoginseng polysaccharide and casein phosphopeptide, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 1:2-4:1-3 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

7. A method for shortening the breeding cycle of golden pomfret as claimed in claim 1, characterized in that: In step (3), the nutrient C is added to the nutrient B: The nutrient material C comprises, by weight: 0.2-0.5 parts of vitamin C, 0.4-0.8 parts of palatinose, 2-4 parts of Polygonum hydropiper extract, 1-3 parts of Acanthopanax senticosus polysaccharide, and 0.8-1.2 parts of freeze-dried yeast powder.

8. A method for shortening the breeding cycle of golden pomfret as claimed in claim 7, characterized in that: The preparation method of the nutrient material C comprises: uniformly mixing vitamin C, palatinose, Polygonum hydropiper extract, Acanthopanax senticosus polysaccharide and yeast freeze-dried powder, puffing and granulating, drying and sieving to obtain particles with a mass ratio of 5-7:1:2-4 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

9. A method for shortening the breeding cycle of golden pomfret as claimed in claim 1, characterized in that: In step (4), the nutrient D is prepared by adding nutrient d to nutrient C: The nutrient material d comprises, by weight: 5-10 parts of Artemia, 1-3 parts of coupled fructose, 8-10 parts of vegetable powder, 0.6-0.8 parts of bio-peptide fermentation liquid, 0.6-0.8 parts of gentian oligosaccharide, 3-5 parts of sea grape extract, and 1-2 parts of Pleurotus eryngii polysaccharide.

10. A method for shortening the breeding cycle of golden pomfret according to claim 9, characterized in that: The preparation method of the nutrient material d comprises: uniformly mixing Artemia, coupled fructose, vegetable powder, biopeptide fermentation broth, oligosaccharides of gentiana, sea grape extract and Pleurotus eryngii polysaccharide, puffing and granulating, drying and screening to obtain particles with a mass ratio of 4-6:1-3:1 and a particle size of 0.5-1.0 mm, a particle size of 1-2 mm and a particle size of 2-4 mm.

Citation Information

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