Hyperbaric oxygen device for treating or improving senile hair abnormality and application and method thereof

Abstract

The invention provides a hyperbaric oxygen device for treating or improving senile hair abnormity and application and a method thereof.The hyperbaric oxygen device comprises a hyperbaric oxygen cabin and a cabin console, the pressure of the cabin console is set to be 1.0-3.0 ATA, the oxygen concentration is larger than or equal to 92%, and the time is 90-180 min; the method comprises the following steps: S1, placing a subject in a hyperbaric oxygen chamber; s2, high-purity oxygen with the oxygen purity larger than or equal to 99.99% is used for continuously ventilating the hyperbaric oxygen chamber till the concentration of replaced oxygen in the hyperbaric oxygen chamber is larger than or equal to 92%; s3, treating for 90-180 minutes under the pressure of 1.0-3.0 ATA, and flushing the interior of the cabin with pure oxygen for 5-10 seconds every 150-300 seconds during the treatment so as to ensure that the oxygen content in the cabin is stable; experiments prove that effective hyperbaric oxygen therapy can improve the problems of senile alopecia, senile poliosis, slow growth of senile hair, abnormal hair follicles and the like, solves the problem of lack of treatment of abnormal hair of old people at present, and can be applied to the fields of beauty treatment and anti-aging.

Description

Technical Field

[0001] The invention relates to a hyperbaric oxygen device for treating or improving abnormal hair in aging and an application and method thereof, belonging to the technical field of aging biology and plastic surgery and cosmetic medicine. Background Art

[0002] As we age, human hair undergoes a series of changes: including decreased hair follicle density (causing thinning hair), hair follicle atrophy (causing thinning hair), graying of hair color (gray hair), a significant slowdown in the start of the hair cycle (slow growth), and more.

[0003] Physiological gray hair (aging gray hair) refers to the decrease in the number of melanocytes after aging, which makes the pigment at the bottom of the hair follicles less and less. The hair loses its original color and becomes lighter and lighter. Eventually, the melanocytes that can secrete pigments disappear completely, and the hair shaft will appear white. At present, there is no effective way to reverse this natural process. Hair dyeing is the most common way to relieve the graying of hair. In addition, the methods of traditional Chinese medicine for the treatment of gray hair to black include: preparing Chinese medicine that nourishes yin and cools blood, nourishes blood and blackens hair, and nourishes the kidney and calms the mind. Such as cooked Shouwu, raw and cooked Rehmannia, American ginseng, wolfberry, Cuscuta, yam, moutan bark, black sesame, Ligustrum lucidum, longan meat, and jujube, boiled in water and taken. Taking Liuwei Dihuang Pills also has a certain auxiliary therapeutic effect. Or, use Chinese medicine angelica, dry pine, gypsum, talc, acid pomegranate peel, mother clove, white sandalwood, no stone, white ginseng and other medicines, grind them into powder, mix them into a paste with rice vinegar, apply them on the hair, wrap them tightly with lotus leaves, and wash off the black the next morning.

[0004] As for the symptoms of hair loss, the mature treatments for hair loss on the market are mainly for androgenic alopecia, and the most mainstream treatment methods are drug therapy (minoxidil, finasteride, etc.) and hair transplantation. However, there are obvious differences in the mechanisms and manifestations of androgenic alopecia and aging-related hair phenotypes. The key factors of the former are genetics and hormones, which usually affect specific parts, while the latter is mainly related to aging and involves general changes in hair color, texture, and density. This makes conventional treatments less applicable to the elderly. Currently, clinical practice focuses more on alleviating hair aging through nutritional supplements and lifestyle adjustments. This treatment status is obviously inconsistent with the current situation of accelerated aging in society and people's high demands for quality of life. Summary of the invention

[0005] The present invention provides a hyperbaric oxygen device for treating or improving abnormal hair growth in the elderly, and its application and method. Experiments have shown that effective hyperbaric oxygen therapy can improve problems such as senile hair loss, senile gray hair, slow senile hair growth and senile hair follicle abnormalities, solves the current lack of treatment for hair abnormalities in the elderly, and can be used in the fields of beauty and anti-aging.

[0006] To achieve this object, the present invention provides the following technical solutions:

[0007] The first aspect of the present invention provides the use of a hyperbaric oxygen device in the preparation of a device for treating or improving aging-related hair abnormalities, characterized in that the aging-related hair abnormalities include aging-related hair loss, aging-related gray hair, aging-related slow hair growth and aging-related hair follicle abnormalities.

[0008] In the present invention, hair follicle abnormalities include decreased hair follicle density, hair follicle atrophy, and the like.

[0009] Preferably, the pressure of the high-pressure oxygen device is set to 1.0-3.0 ATA, and the oxygen concentration is set to ≥92%; further preferably, the pressure of the high-pressure oxygen device is set to 2.0 ATA, and the oxygen concentration is set to ≥92%.

[0010] Preferably, the use time of the hyperbaric oxygen device is set to 90-180 minutes each time; further preferably, the use time of the hyperbaric oxygen device is set to 90 minutes each time.

[0011] Preferably, the high-pressure oxygen device is used at a frequency of not less than 6 times per week, and the interval between two adjacent high-pressure oxygen treatments is not less than 24 hours.

[0012] Preferably, the period of hyperbaric oxygen therapy can be determined according to the specific conditions of the subject. Generally, each treatment lasts no less than 4 weeks.

[0013] The second aspect of the present invention provides a hyperbaric oxygen device for treating or improving abnormal hair in aging, comprising a hyperbaric oxygen chamber and a chamber console, wherein the chamber console is set to a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.

[0014] Further preferably, the cabin console is set to a pressure of 2.0 ATA, an oxygen concentration ≥ 92%, and a time of 90 min.

[0015] Preferably, the senile hair abnormalities include senile hair loss, senile gray hair, senile slow hair growth and senile hair follicle abnormalities.

[0016] The third aspect of the present invention provides a method for improving abnormal hair in aging for non-therapeutic purposes, comprising the following steps:

[0017] S1. Place the subject in a hyperbaric oxygen chamber;

[0018] S2. Use high-purity oxygen with an oxygen purity of ≥99.99% to continuously ventilate the hyperbaric oxygen chamber until the replacement oxygen concentration in the hyperbaric oxygen chamber reaches ≥92%;

[0019] S3. Perform treatment for 90-180 minutes at a pressure of 1.0-3.0ATA. During this period, flush the chamber with pure oxygen for 5-10 seconds every 150-300 seconds to ensure a stable oxygen content in the chamber.

[0020] Preferably, the method further comprises the following steps: repeating steps S1-S3 at least 6 times per week, with the interval between two adjacent steps being at least 24 hours.

[0021] Preferably, the senile hair abnormalities include senile hair loss, senile gray hair, senile slow hair growth and senile hair follicle abnormalities.

[0022] In the present invention, hyperbaric oxygen medicine originated in the 17th century and was initially used to treat hypoxia and ischemic diseases. In the past 60 years, it has experienced a rise and flourishing development in modern medicine, and its indications have been continuously expanded. The application experience of modern medicine in hyperbaric oxygen has fully proved its safety and non-invasiveness. Therefore, using hyperbaric oxygen as a means to improve aging hair abnormalities for non-therapeutic purposes has a good safety basis.

[0023] In the present invention, the improvement of hair abnormalities in aging for non-therapeutic purposes includes, but is not limited to, intervention in the fields of beauty and hair loss for problems such as hair loss and gray hair in the elderly.

[0024] Compared with the prior art, the beneficial effects and significant progress of the technical solution of the present invention are: the hyperbaric oxygen device for treating or improving aging hair abnormalities provided by the present invention can significantly reverse aging hair abnormalities, including hair graying, significantly slower hair growth, hair thinning, hair follicle abnormalities, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solution of the present invention, the drawings used in the embodiments of the present invention are briefly introduced below.

[0026] Figure 1 This is an in situ photograph of a mouse cage on the 12th day after wax depilation in Example 1;

[0027] Figure 2 are the skin and hair phenotypes of the three groups of mice in Example 2;

[0028] Figure 3 is the hair follicle staining image of three groups of mice in Example 3;

[0029] Figure 4 is a graph of bulk RNA sequencing results of Example 4, wherein a is a heat map, b is a Venn diagram, and c is a gene set enrichment map;

[0030] Figure 5 This is a diagram of the Gene Ontology (GO) analysis results of Example 4. DETAILED DESCRIPTION

[0031] To make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the present invention is further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are weight percentages and weight parts. The experimental materials and reagents used in the following examples can be obtained from commercial channels unless otherwise specified.

[0032] Unless otherwise specified, the technical and scientific terms used herein have the same meaning as commonly understood by ordinary technicians in the technical field to which the application belongs. It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments of the present application.

[0033] In order to more fully understand the present invention, the professional terms in the present invention are explained below.

[0034] Aging refers to natural aging, generally referring to people over 60 years old.

[0035] Hair follicle density. There are approximately 80 to 140 hair follicles in one square centimeter of hair follicle density. It is abnormal if there are too many or too few hairs in the follicles.

[0036] Hair follicle atrophy is mainly caused by hair follicle degeneration. During the growth and aging process, hair follicles will also atrophy or age. In particular, excessive life or mental stress, environmental factors, pollution from chemical shampoos, nutritional imbalance, and insufficient blood supply can cause the hair follicle environment to deteriorate severely, resulting in hair follicle blockage, which affects the hair roots' absorption of nutrients and causes hair loss. In the prior art, there is no very effective treatment for hair follicle atrophy. If it causes total baldness, hair follicle transplantation can be performed.

[0037] Bulk RNA sequencing is the process of extracting mixed RNA from tissues, organs or a group of cells for sequencing. The average data of the transcriptome of a group of cells, or representative data, can be obtained.

[0038] Heatmap, indicating the expression level of a gene at a certain position in the map. Cluster heatmap can be used to determine the expression pattern of differential genes under different experimental conditions. Each comparison combination will get a differential gene set, and the FPKM value of the union of the differential gene sets of all comparison combinations in each experimental group / sample is used for hierarchical clustering, K-means clustering and SOM clustering analysis. Heatmap can be implemented using pheatmap in the R language tool.

[0039] Venn diagram, also known as Venn diagram, is a diagram used to display the overlapping areas of element sets, usually represented by circles or ellipses. According to the number of participating sets, the Venn diagram can be divided into two sets, three sets, four sets, five sets, etc. It can be implemented using R language, such as using the Vennerable package in R language. Each ellipse represents a differentially expressed gene in a comparison combination (treatment group vs. control group), and the numbers in the overlapping areas of the ellipses represent the number of common differentially expressed genes between the corresponding multiple comparison combinations, and the non-overlapping areas represent the differentially expressed genes unique to each comparison combination. The table corresponding to the Venn diagram can be used to see the common and unique gene information of the comparison combination.

[0040] Gene Set Enrichment Analysis (GSEA) is an enrichment method proposed by the Broad Institute. It also provides the corresponding analysis software GSEA and a gene set database. GSEA analyzes whether all genes in a gene set are enriched at the top or bottom of the sorted list. If they are enriched at the top, it can be said that, overall, the gene set is up-regulated. Conversely, if they are enriched at the bottom, they are down-regulated.

[0041] Gene Ontology (GO) is an ontology widely used in the field of bioinformatics, which covers three aspects of biology: cellular components, molecular functions, and biological processes.

[0042] Example 1

[0043] Experimental methods

[0044] 1.1. Construction of natural aging model

[0045] Unbred C57BL / 6J male mice were housed in an SPF animal room until they were 20 months old.

[0046] 1.2 Experimental Grouping

[0047] In order to illustrate the effect of hyperbaric oxygen therapy, two groups were set up and given different treatment plans (n=5). The OLD+HBO group was naturally aged mice treated with hyperbaric oxygen, and the OLD group was naturally aged mice treated with sham treatment. They were placed in the same ventilation cage and in the animal experimental chamber for the same time, but without ventilation treatment.

[0048] 1.3. Hyperbaric oxygen treatment

[0049] From the age of 20 months, the naturally aged model mice were treated with hyperbaric oxygen 6 times a week for 4 weeks, with an interval of ≥24 hours between two hyperbaric oxygen treatments. Hyperbaric oxygen treatment was carried out in the animal experimental chamber. The treatment plan included: placing C57BL / 6J mice in a closed ventilation cage and placing them in the chamber; using high-purity oxygen (Shanghai Chloromin Gas Co., Ltd., oxygen purity ≥99.99%) to continuously ventilate the animal experimental chamber until the oxygen concentration in the chamber was replaced to ≥92%, which took 20-30 minutes; setting automatic parameters and starting the program: the pressure increase and decompression time were 10 minutes respectively, and HBO treatment was performed for 90 minutes at a pressure of 2.0ATA, and the chamber was flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure the stability of the oxygen content in the chamber.

[0050] 1.4 Waxing

[0051] After the last hyperbaric oxygen treatment, the aged mice continued to be raised in the animal room for 4 weeks, followed by uniform wax depilation. The mice were first injected with anesthetics intraperitoneally, and after the mice were anesthetized, wax depilation was repeated three times on the back skin of each mouse. The specific operation each time was: turn on the matching heater in advance to melt the Brazilian hard wax (Dexin) into a viscous yellow liquid, use a wooden stick to scrape an appropriate amount of hot wax, wait for 3-5 seconds to cool it slightly to avoid burns, and then apply it evenly on the back skin of the mouse. After 5-10 seconds, the wax solidifies and then the wax block is torn off. After three consecutive wax depilations on the same area, ensure that the hair is removed to achieve basic synchronization of the hair cycle.

[0052] 1.5. Observe and take photos of the mouse back skin.

[0053] Experimental Results

[0054] The results are as follows Figure 1 The following is a picture of mice in situ in their cages on day 12 after wax depilation. Figure 1 It can be clearly observed that the hair of the elderly mice in the OLD+HBO group was denser.

[0055] Example 2

[0056] Experimental grouping: Two groups were set up and given different treatments (n=5). The OLD+HBO group consisted of naturally aged mice treated with hyperbaric oxygen, and the OLD group consisted of naturally aged mice treated with sham treatment. They were placed in the same ventilation cage and in the animal experimental chamber for the same period of time, but without ventilation treatment. The YNG group consisted of 2-month-old young mice treated with sham treatment.

[0057] Experimental methods: The construction of the natural aging model, hyperbaric oxygen treatment and wax depilation were the same as those in Example 1.

[0058] The experimental results are as follows Figure 2As shown in the figure, the skin and hair phenotype of naturally aged mice were observed and analyzed in more detail. Two-month-old young mice were used as controls, and the mice were photographed (14 days after waxing) ( Figure 2 , left) and photographed under a hair microscope (on the day of waxing and 14 days after waxing) ( Figure 2 , right). Microscopic examination showed that waxing completely removed the skin hair. 14 days after waxing, the hair of young mice (YNG) was vigorous and dark in color, while the hair of old mice (OLD) was still short and obviously sparse, while the hair of old mice treated with hyperbaric oxygen (OLD+HBO) was significantly longer, with increased hair density and darker color.

[0059] Example 3

[0060] The experimental grouping and experimental methods of this example are the same as those of Example 2. The naturally aged mice and young control mice of the same batch treated with or without hyperbaric oxygen and young control mice were killed and dissected 1-2 weeks after the end of the hyperbaric oxygen treatment. The dorsal skin (without wax depilation) was taken to make paraffin sections, which were then stained with hematoxylin and eosin (HE).

[0061] The results are as follows Figure 3 As shown, compared with young mice, the skin of old mice showed pathological manifestations such as hair follicle atrophy, decreased hair follicle density, abnormal surface keratinization, and disordered dermal structure. Compared with old mice without hyperbaric oxygen treatment, the skin section morphology of old mice after hyperbaric oxygen treatment was significantly closer to that of young mice, with higher hair follicle density, more normal hair follicle morphology, thinner stratum corneum, and clearer dermal structure.

[0062] Example 4

[0063] The experimental grouping and experimental methods of this example are the same as those of Example 2. The naturally aged mice and young control mice of the same batch treated with or without hyperbaric oxygen and young control mice were killed and dissected 1-2 weeks after the end of the hyperbaric oxygen treatment, and skin tissues were taken for bulk RNA sequencing, and the sequencing results were subjected to bioinformatics analysis.

[0064] The results are as follows Figure 4 As shown, the heat map shows (Figure a) that hyperbaric oxygen treatment rescued the aging changes in mouse skin and hair, including reversing the high expression of skin aging-related proteins and increasing the expression level of Lgr6 (a hair follicle stem cell marker). The Venn diagram showed that the differential genes in the skin of old mice treated with hyperbaric oxygen compared with those in the skin of old mice compared with those in the skin of young mice overlapped significantly (Figure b). Gene set enrichment analysis (GSEA) also suggested (Figure c) that the aging changes in the skin of old mice were significantly reversed by hyperbaric oxygen.

[0065] Gene Ontology (GO) analysis showed that Figure 5As shown in the figure, compared with old mice, the GO items that were significantly upregulated in the skin of old mice treated with hyperbaric oxygen were mostly related to hair, including hair cycle (BP_molting cycle and BP_haircycle), skin development (BP_skin development), melanin production related to hair color (BP_melaninbiosynthetic process), and BMP signaling pathway (BP_BMPsignaling pathway and MF_BMP receptor binding) closely related to the regulation of hair growth cycle, while the items downregulated after hyperbaric oxygen treatment were related to inflammation and keratinization.

[0066] The applicant declares that, in the description of the above specification:

[0067] The descriptions of the terms "this embodiment", "an embodiment of the present invention", "as shown in...", "further", "a further improved technical sub-scheme", etc., mean that the specific features, structures, materials or characteristics described in the embodiment or example are included in at least one embodiment or example of the present invention; in this specification, the schematic expressions of the above terms are not necessarily for the same embodiment or example, and the specific features, structures, materials or characteristics described may be combined or combined in a suitable manner in any one or more embodiments or examples; in addition, a person of ordinary skill in the art may combine or combine the different embodiments or examples and the features of the different embodiments or examples described in this specification without causing any contradiction.

[0068] Finally, it should be noted that:

[0069] The above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them.

[0070] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein, and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. The non-essential improvements, adjustments or replacements made by those skilled in the art based on the contents of this specification are all within the scope of protection required by the present invention.

Claims

1. Use of a hyperbaric oxygen device in the preparation of a device for treating or improving abnormal hair growth in aging, characterized in that: The senile hair abnormalities include senile hair loss, senile gray hair, senile slow hair growth and senile hair follicle abnormalities.

2. The use according to claim 1, characterized in that The pressure of the high-pressure oxygen device is set to 1.0-3.0 ATA, and the oxygen concentration is set to ≥92%.

3. The use according to claim 1, characterized in that The use time of the hyperbaric oxygen device is set to 90-180 minutes each time.

4. The use according to claim 1, characterized in that The frequency of use of the hyperbaric oxygen device is no less than 6 times per week, and the interval between two adjacent hyperbaric oxygen treatments is no less than 24 hours.

5. A hyperbaric oxygen device for treating or improving abnormal hair growth in aging, characterized in that: It comprises a hyperbaric oxygen chamber and a chamber console, wherein the chamber console is set to have a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.

6. The hyperbaric oxygen device for treating or improving abnormal hair growth in aging as claimed in claim 5, characterized in that: The senile hair abnormalities include senile hair loss, senile gray hair, senile slow hair growth and senile hair follicle abnormalities.

7. A method for improving hair abnormalities in aging for non-therapeutic purposes, characterized in that: The following steps are involved: S1. Place the subject in a hyperbaric oxygen chamber; S2. Use high-purity oxygen with an oxygen purity of ≥99.99% to continuously ventilate the hyperbaric oxygen chamber until the replacement oxygen concentration in the hyperbaric oxygen chamber reaches ≥92%; S3. Treatment is carried out for 90-180 minutes at a pressure of 1.0-3.0ATA. During this period, the chamber is flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure that the oxygen content in the chamber is stable.

8. The method for improving hair abnormalities in aging for non-therapeutic purposes according to claim 7, characterized in that: The following steps are also included: Repeat steps S1-S3 at least 6 times a week, with a gap of at least 24 hours between two consecutive times.

9. The method for improving hair abnormalities in aging for non-therapeutic purposes according to claim 7, characterized in that: The senile hair abnormalities include senile hair loss, senile gray hair, senile slow hair growth and senile hair follicle abnormalities.

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