Application of raoultella ornithinolytica product in degradation of animal waste
By using specific concentrations of ornithine-degraded Raulis products in livestock and poultry manure for fermentation, the pollution problems and insufficient resource utilization in livestock and poultry manure treatment are solved, and the rapid decomposition and efficient fermentation of the manure is achieved, and the rapid decomposition and efficient fermentation of the manure is improved. The content of nutrients.
Patent Information
- Application Number
- CN202411240077.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-05
- Publication Date
- 2024-12-13
AI Technical Summary
The prior art has failed to effectively solve the pollution problem in the treatment of livestock and poultry manure and failed to make full use of biomass resources, resulting in the pollution problem in the livestock and poultry breeding industry not being completely solved.
Using specific concentrations of Raoeria ornithine products, fermentation is carried out by regulating the moisture of animal feces and adding Raoeria ornithine to promote the decomposition and compost fermentation process of the feces, shortening the fermentation time and improving nutrition. Content of ingredients.
The decomposition and fermentation speed of feces is accelerated, the moisture content of materials after fermentation is reduced, the content of nutrients such as nitrogen, phosphorus and potassium is increased, and the resource utilization of feces is promoted.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of animal excrement treatment, in particular to the application of Raoultella ornithine-degrading products in degrading animal excrement. Background Art
[0002] The current treatment and utilization of manure is mainly concentrated in the direction of feed, fertilizer and biogas production. It mainly uses aerobic or anaerobic fermentation to ferment and degrade livestock and poultry manure. The fermentation treatment method has the advantages of producing bioenergy, low demand for nitrogen and phosphorus nutrients, and good degradation ability for certain organic matter. Although there are many ways to treat manure, a considerable number of livestock and poultry farms have not yet met the national emission standards for the comprehensive utilization and treatment of manure, which also makes livestock and poultry manure waste an underutilized biomass resource library. Therefore, how to effectively solve the pollution problem of the breeding industry while taking into account economic development has become an urgent problem to be solved in the breeding industry. Summary of the invention
[0003] The purpose of the present invention is to provide an application of an ornithine-degrading Raoultella product in the degradation of animal feces to solve the problems existing in the above-mentioned prior art. The present invention verifies that a specific concentration of ornithine-degrading Raoultella can accelerate the maturity of animal feces, accelerate the composting fermentation speed, and shorten the composting fermentation time.
[0004] To achieve the above object, the present invention provides the following solutions:
[0005] The present invention provides the use of an ornithine-degrading Raoultella product in degrading animal feces. The ornithine-degrading Raoultella product comprises a Raoultella ornithine-degrading bacterial liquid.
[0006] Furthermore, the concentration of the ornithine-degrading Raoultella solution is 2×10 8 cfu / mL-2×10 9 cfu / mL.
[0007] Further, the method comprises the following steps: adjusting the moisture content of the animal feces to 50-55 wt % to obtain a pretreated material;
[0008] Add the Raoultia ornithine-degrading bacterial solution to the pretreated material and ferment for 15-20 days.
[0009] Furthermore, the volume mass ratio of the Raoultella ornithine-degrading bacterial liquid to the animal feces is 1 L: (10-20) kg.
[0010] Furthermore, the volume mass ratio of the Raoultella ornithine-degrading bacterial liquid to the animal feces is 1L:10kg.
[0011] Furthermore, the moisture content of the animal feces is adjusted to 55 wt %; and the fermentation time is 15 days.
[0012] Furthermore, the step of adjusting the moisture content of the animal manure includes adding wheat straw.
[0013] Furthermore, the animal includes a pig or a chicken.
[0014] The present invention discloses the following technical effects:
[0015] The present invention has been experimentally verified to find that a specific concentration of Raoultella ornithine-degrading bacterial liquid can promote the maturity of animal manure, accelerate the composting fermentation speed, shorten the composting fermentation time, and simultaneously reduce the water content of the fermented material, effectively promote the degradation of organic matter in animal manure, and increase the content of nutrients such as nitrogen, phosphorus and potassium, which has important practical significance for the resource utilization of agricultural waste. DETAILED DESCRIPTION
[0016] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0017] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.
[0018] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.
[0019] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.
[0020] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.
[0021] The Raoultella ornithinolytica used in the present invention has a deposit number of GDMCC NO.1.1019, which was purchased from Guangdong Microbiological Culture Collection Center. The public can purchase the strain from the above depository. The specific research is as follows.
[0022] Example 1
[0023] The ornithine-solubilizing Raoultella was inoculated into NA medium and cultured at 37°C for 12 hours to obtain activated ornithine-solubilizing Raoultella; the activated ornithine-solubilizing Raoultella was then inoculated into LB liquid medium at an inoculum amount of 5% (v / v), and cultured in a shaking incubator for 36 hours at a shaking speed of 150 rpm and a culture temperature of 37°C to obtain a Raoultella ornithine-solubilizing bacterial solution with a concentration of 2×10 9 cfu / mL.
[0024] 100 kg of pig manure and 20 kg of wheat straw were placed in a fermentation tank, the water content was adjusted to 55 wt%, 10 L of Raoultella ornithine-degrading bacterial solution was added, the mixture was mixed evenly, and the mixture was fermented for 15 days.
[0025] Example 2
[0026] The same as Example 1, except that the concentration of the ornithine-degrading Raoultella solution was adjusted to 2×10 8 cfu / mL before proceeding with subsequent operations.
[0027] Comparative Example 1
[0028] The same as Example 1, except that the concentration of the ornithine-degrading Raoultella solution was adjusted to 2×10 6 cfu / mL before proceeding with subsequent operations.
[0029] Comparative Example 2
[0030] 100 kg of pig manure and 20 kg of wheat straw were placed in a fermentation tank, the moisture was adjusted to 55 wt%, mixed evenly, and fermented for 15 days.
[0031] Effect verification example 1: Effect on the degradation of pig feces
[0032] On the 1st, 3rd, 5th, 7th, 9th, 12th and 15th days, the pig manure compost samples were collected from each case. Each time, 50g of samples were randomly collected from the east, south, west, north and center of the pile and placed in sterile ziplock bags. The temperature change was monitored by a temperature sensor, and the temperature on the 1st, 3rd, 5th, 7th, 9th, 12th and 15th days was recorded. The C / N ratio change of the pile on the 1st, 3rd, 5th, 7th, 9th, 12th and 15th days was detected by an element analyzer, and the results are as follows.
[0033] 1. Impact on temperature
[0034] Compost temperature is an important indicator for judging the maturity of compost from the surface. Temperature controls the fermentation process by affecting the activity of microorganisms and the degradation rate of organic matter. As shown in Table 1, the temperature rise period of Example 1 and Example 2 was 1-5 days, and both reached the highest temperature on the 5th day, which was 67°C and 65°C respectively. The temperature rise period of Comparative Example 1 was 1-7 days, and reached the highest temperature of 59°C on the 7th day, while the temperature of Comparative Example 2 reached the highest temperature on the 9th day, which was 55°C. The compost of the Example group heated up early and quickly, and the concentration of 2×10 8 cfu / mL or 2×10 9 cfu / mL of Raoultella ornithine-degrading bacteria solution can increase the temperature peak, and the high temperature lasts longer, the decomposition effect is better, the composting fermentation speed is accelerated, and the composting fermentation time is shortened; the comparative example 1 group is added with a concentration of 2×10 6 The composting effect of the Raoultella ornithine-lytica liquid with a concentration of cfu / mL was obviously not as good as that of the Example group, but was better than that of the Comparative Example 2 group to which no bacterial liquid was added.
[0035] Table 1 Effect of temperature
[0036]
[0037]
[0038] 2. Impact on C / N ratio
[0039] The C / N ratio is one of the common methods for evaluating the fermentation maturity of poultry and livestock manure. The results are shown in Table 2. During the entire composting reaction process, the C / N ratio of the four groups continued to decrease, and the C / N decreased faster during the warming period and the high temperature period. The ratio T is the ratio of C / N at the end of composting to C / N at the beginning of composting. The T value is one of the indicators to measure whether the entire pile is mature. If the T value is controlled at 0.5-0.75, it can be considered that the pile has basically completed the composting process. The lower the T value, the higher the maturity of the pile. The T values of the four groups are 0.640, 0.645, 0.690, and 0.717, respectively. According to the T value maturity judgment range, the four groups basically reach maturity, and the degree of maturity is ranked as Example 1 Group> Example 2 Group> Comparative Example 1 Group> Comparative Example 2 Group. The results show that adding a specific concentration of ornithine-decomposing Raoultella liquid can improve the maturity of pig manure in the pile.
[0040] Table 2 Impact on C / N ratio
[0041]
[0042] 3. Impact on nutrients
[0043] After the fermentation was completed, the moisture content, organic matter, total nitrogen, total phosphorus and total potassium content of the pig manure were determined using the agricultural standard NY525-2002. The results are shown in Table 3.
[0044] Table 3 Effects on nutritional components
[0045] Group Water content / % Organic matter / % Total nitrogen / % Total phosphorus / % Total potassium / % Example 1 21 10.3 1.65 1.62 1.51 Example 2 22 10.5 1.58 1.56 1.50 Comparative Example 1 26 14.1 1.06 0.97 1.02 Comparative Example 2 29 15.6 0.92 0.86 0.82
[0046] The results showed that the addition concentration was 2×10 8 cfu / mL or 2×10 9 cfu / mL of Raoultella ornithine degrading bacteria had a better effect on the degradation of organic matter in pig feces. The organic matter content was lower than that of the control group, and the nitrogen, phosphorus and potassium content was higher than that of the control group, indicating that a specific concentration of Raoultella ornithine degrading bacteria could effectively promote the degradation of organic matter in pig feces. 6 Although the effect of the comparative example 1 group containing the Raoultella ornithine-lysing bacterial solution of 1.5 cfu / mL is not as good as that of the example 1 group and the example 2 group, it also has an improvement effect on the changes of various nutritional components of pig manure compared with the comparative example 2 group without adding bacterial solution.
[0047] Example 3
[0048] The same as Example 1, the only difference is that the pig manure is replaced by chicken manure.
[0049] Example 4
[0050] The same as Example 3, except that the concentration of the ornithine-degrading Raoultella solution was adjusted to 2×108 cfu / mL before proceeding with subsequent operations.
[0051] Comparative Example 3
[0052] The same as Example 3, except that the concentration of the ornithine-degrading Raoultella solution was adjusted to 2×10 6 cfu / mL before proceeding with subsequent operations.
[0053] Comparative Example 4
[0054] 100 kg of chicken manure and 20 kg of wheat straw were placed in a fermentation tank, the moisture was adjusted to 55 wt%, mixed evenly, and fermented for 15 days.
[0055] Effect verification example 2: Effect on the degradation of chicken manure
[0056] The operation was the same as that of “Effect Verification Example 1 on the Degradation of Pig Manure”. The effects of different treatments on the temperature, C / N ratio and nutritional components of chicken manure are shown in Tables 4, 5 and 6.
[0057] 1. Impact on temperature
[0058] As shown in Table 4, the temperature rise period of Example 3 and Example 4 was 1-5 days, and both reached the highest temperature on the 5th day, which was 66°C and 65°C respectively. The temperature rise period of Comparative Example 3 was 1-7 days, and reached the highest temperature of 59°C on the 7th day, while the temperature of Comparative Example 4 reached the highest temperature on the 9th day, which was 54°C. The compost of the Example group heated up early and quickly, and the concentration of 2×10 8 cfu / mL or 2×10 9 cfu / mL of Raoultella ornithine-degrading bacterial liquid can increase the temperature peak, and the high temperature lasts for a longer time, the decomposition effect is better, the composting fermentation speed is accelerated, and the composting fermentation time is shortened; the composting effect of comparative example 3 group is obviously not as good as that of the embodiment group, but is worse than that of comparative example 4 group without adding bacterial liquid.
[0059] Table 4 Effect of temperature
[0060]
[0061]
[0062] 2. Impact on C / N ratio
[0063] The results are shown in Table 5. During the entire composting reaction process, the C / N ratio of the four groups continued to decrease, and the C / N decreased faster during the temperature rise period and the high temperature period. The T values of the four groups were 0.688, 0.700, 0.796, and 0.825, respectively. According to the T value maturity determination range, the two groups of Example 3 and Example 4 were basically mature, while the two groups of Comparative Example 3 and Comparative Example 4 were not mature. The results show that adding a specific concentration of Raoultella ornithine-degrading bacteria liquid can improve the maturity of chicken manure in the pile.
[0064] Table 5 Impact on C / N ratio
[0065]
[0066] 3. Impact on nutrients
[0067] Table 6 Effects on nutritional components
[0068] Group Water content / % Organic matter / % Total nitrogen / % Total phosphorus / % Total potassium / % Example 3 19 20.5 2.78 2.74 1.55 Example 4 20 20.4 2.49 2.47 1.54 Comparative Example 3 27 24.4 1.85 1.73 0.99 Comparative Example 4 33 25.6 1.65 1.53 0.85
[0069] The results are shown in Table 6. The Example 3 and Example 4 groups have better degradation effects on organic matter in chicken manure, the organic matter content is lower than that of the comparative example group, and the nitrogen, phosphorus and potassium content is higher than that of the comparative example group, indicating that a specific concentration of Raoultella ornithine-degrading bacteria liquid can effectively promote the degradation of organic matter in pig manure. Although the effect of the comparative example 3 group is not as good as that of the Example 3 and Example 4 groups, it also has an improvement effect on the changes in various nutritional components of chicken manure compared with the comparative example 4 group without adding bacteria liquid.
[0070] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.
Claims
1. The use of a Raoultella ornithine-degrading product in degrading animal feces, characterized in that: The Raoultia ornithine-lytica product comprises Raoultia ornithine-lytica bacterial liquid.
2. The use according to claim 1, characterized in that The concentration of the ornithine-degrading Raoultella solution is 2×10 8 cfu / mL-2×10 9 cfu / mL.
3. The use according to claim 1, characterized in that The following steps are involved: Adjusting the moisture content of the animal manure to 50-55 wt % to obtain a pretreated material; Add the Raoultia ornithine-degrading bacterial solution to the pretreated material and ferment for 15-20 days.
4. The use according to claim 3, characterized in that The volume mass ratio of the Raoultella ornithine-degrading bacterial liquid to the animal feces is 1 L: (10-20) kg.
5. The use according to claim 4, characterized in that The volume mass ratio of the Raoultella ornithine-degrading bacterial liquid to the animal feces is 1 L:10 kg.
6. The use according to claim 3, characterized in that Adjusting the moisture content of the animal feces to 55 wt %; The fermentation time is 15 days.
7. The use according to claim 3, characterized in that The step of adjusting the moisture content of the animal manure includes adding wheat straw.
8. The use according to claim 1, characterized in that The animal includes a pig or a chicken.