Blood-imitating fluid and preparation method thereof

Abstract

The invention discloses a simulated blood fluid and a preparation method thereof. The simulated blood fluid comprises the following components in percentage by mass: 20-40 wt.% of polyvinylpyrrolidone and the balance of a solvent. The simulated blood fluid provided by the invention is a non-Newtonian fluid, does not precipitate, and can be applied to photoacoustic / ultrasonic dual-mode imaging.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and more specifically, to a blood-simulating fluid and a preparation method thereof. Background Art

[0002] B-ultrasound products are one of the commonly used imaging diagnostic equipment in clinical practice. B-ultrasound phantoms have similar ultrasonic characteristics to biological tissues and are mainly used for correction, inspection and in vitro learning and training of B-ultrasound products. B-ultrasound phantoms have important practical significance for the evaluation of the quality and performance of B-ultrasound products.

[0003] The cardiovascular B-ultrasound phantom includes a heart phantom with similar ultrasonic characteristics to heart tissue, simulated blood vessels in the phantom, and simulated blood fluid in the simulated blood vessels. The peristaltic pump is used to make the simulated blood fluid flow in the simulated blood vessels to simulate the blood flow in the heart. The cardiovascular B-ultrasound phantom can intuitively reflect the blood flow, blood vessel conditions, blood flow direction, and heart valve conditions in the color ultrasound Doppler mode.

[0004] The blood-mimicking fluid imitates blood, which includes plasma and red blood cells dissolved in the plasma. Plasma is a Newtonian fluid, and since red blood cells can be deformed, blood is a typical non-Newtonian fluid.

[0005] The blood-mimicking fluid in the prior art is usually a suspension fluid composed of water and nanoparticles such as aluminum oxide or graphite powder dispersed in water. Water is used to simulate plasma, and nanoparticles are used to simulate red blood cells. Nanoparticles can reflect ultrasound to form ultrasonic signals, so that the flow of blood can be observed in the color ultrasound Doppler mode. However, the existing blood-mimicking fluid has the following disadvantages: first, it is a Newtonian fluid, and the fluid properties are different from those of non-Newtonian fluids. When it is used to simulate non-Newtonian fluid blood, the simulation results are inaccurate; second, the suspension is prone to precipitation, which affects the test results of blood vessels and blood flow; third, nanoparticles are opaque and cannot be used for photoacoustic detection. Summary of the invention

[0006] The purpose of the present invention is to overcome the above-mentioned defects of the prior art and provide a blood-mimicking fluid and a preparation method thereof, which is not only a non-Newtonian fluid and does not cause precipitation, but also can be applied to both photoacoustic and ultrasonic modes.

[0007] To achieve the above object, the technical solution of the present invention is as follows:

[0008] A blood-mimicking fluid comprising the following components in percentage by mass:

[0009] 20wt.% to 40wt.% of polyvinyl pyrrolidone and the balance is solvent.

[0010] The present invention also discloses a method for preparing the above-mentioned blood-mimicking fluid, comprising the following steps:

[0011] Polyvinyl pyrrolidone is dissolved in a solvent to obtain the blood-mimicking fluid.

[0012] Implementing the embodiments of the present invention will have the following beneficial effects:

[0013] The polyvinyl pyrrolidone solution of the embodiment of the present invention is a non-Newtonian fluid. Polyvinyl pyrrolidone can reflect ultrasound to form an ultrasonic signal, and the polyvinyl pyrrolidone solution will not precipitate. At the same time, polyvinyl pyrrolidone is also light-transmissive and can be used for both photoacoustic and ultrasonic detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] in:

[0016] Figure 1 This is a photograph of the blood-simulating fluid of Example 3 when it is poured.

[0017] Figure 2 This is a photograph of the blood-simulating fluid of Example 3 showing a siphon effect.

[0018] Figure 3 This is a photograph of the blood-simulated fluid ultrasound imaging of Example 3.

[0019] Figure 4 1 is a comparison of the acoustic attenuation coefficient curves of the blood-simulating fluid of Example 3 and human blood. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The present invention discloses a blood-simulating fluid, which comprises the following components in percentage by mass:

[0022] 20wt.% to 40wt.% of polyvinyl pyrrolidone and the balance is solvent.

[0023] In the above embodiment, the polyvinyl pyrrolidone solution is a non-Newtonian fluid. The polyvinyl pyrrolidone can reflect ultrasound to form an ultrasonic signal, and the polyvinyl pyrrolidone solution will not precipitate. At the same time, the polyvinyl pyrrolidone is also light-transmissive and can be used for both photoacoustic and ultrasonic detection.

[0024] In the above technical solution, the solvent is a solvent that can dissolve polyvinyl pyrrolidone, which can be a polar solvent or an organic solvent. By adjusting the content of polyvinyl pyrrolidone and selecting a suitable solvent, the viscosity, density, sound velocity and sound attenuation of the blood-like fluid can be adjusted to be similar to those of blood, so as to obtain the ability to

[0025] In a specific embodiment, the solvent includes one or more of water, glucose, physiological saline, etc. Aqueous solution is preferred, which has the advantages of low cost, non-toxicity, good biocompatibility, etc.

[0026] In a specific embodiment, the average molecular weight of polyvinyl pyrrolidone is 8000-1200000. Specifically, it can include one or more of PVP K15 (average molecular weight 8000-12000), PVP K17 (average molecular weight 10000-16000), PVP K25 (average molecular weight 30000-40000), PVP K30 (average molecular weight 45000-58000), PVP K60 (average molecular weight 270000-400000), and PVP K90 (average molecular weight 1000000-1300000).

[0027] Preferably, the average molecular weight of polyvinyl pyrrolidone is 30,000 to 58,000, and specifically, PVP K25 and / or PVP K30 are preferred.

[0028] In a specific embodiment, the blood-simulating fluid further includes 3 wt.% to 5 wt.% of a thickener, and the thickener is mainly used to adjust the viscosity of the fluid.

[0029] Specifically, in a specific embodiment, the thickener may include one or more of sodium hydroxymethyl cellulose, hydroxyethyl methacrylate, and sodium alginate.

[0030] In a specific embodiment, the blood-simulating fluid also includes 0.1 wt.% to 0.5 wt.% of a defoaming agent, which is mainly used to eliminate tiny bubbles generated by the peristaltic pump driving the liquid circulation, maintain the stability of the fluid system, and avoid affecting the ultrasonic image.

[0031] Specifically, in a specific embodiment, the defoaming agent may include one or more of emulsified silicone oil and dimethylsiloxane polymer.

[0032] In a specific embodiment, the blood-simulating fluid further includes 0.1% to 0.5% of a dye, which can be dyed into a corresponding color as required. The dye is preferably soluble in a solvent to avoid precipitation.

[0033] Preferably, the viscosity of the simulated blood fluid is 3cP~5cP; the speed of sound is 1570m / s~1600m / s; the sound attenuation is less than 0.1dB / cm / MHz; the density is 1g / cc~1.1g / cc, which is close to real blood in the human body and can more accurately simulate blood flow in color ultrasound Doppler mode.

[0034] The present invention also discloses a method for preparing the above-mentioned blood-mimicking fluid, comprising the following steps:

[0035] Polyvinyl pyrrolidone is dissolved in a solvent to obtain a blood-mimicking fluid.

[0036] When the blood-simulating fluid also includes a thickener, a defoamer and a dye, all the components can be added to the solvent together for dissolution, or they can be added step by step for dissolution. Of course, the dissolution process can also be accelerated by methods such as ultrasound, agitators, heating, etc.

[0037] The following are specific embodiments.

[0038] Example 1

[0039] A blood-mimicking fluid comprising the following components in percentage by mass:

[0040] 20wt.% PVP K30 and the balance water solvent.

[0041] Example 2

[0042] A blood-mimicking fluid comprising the following components in percentage by mass:

[0043] 40wt.% PVP K30 and the balance water solvent.

[0044] Example 3

[0045] A blood-mimicking fluid comprising the following components in percentage by mass:

[0046] 20wt.% PVP K30, 3wt.% CMC (sodium hydroxymethylcellulose), 0.3wt.% emulsified silicone oil and the balance water solvent.

[0047] Example 4

[0048] A blood-mimicking fluid comprising the following components in percentage by mass:

[0049] 20wt.% PVP K30, 3wt.% hydroxyethyl methacrylate, 0.3wt.% emulsified silicone oil and the balance water solvent.

[0050] Example 5

[0051] A blood-mimicking fluid comprising the following components in percentage by mass:

[0052] 20wt.% PVP K30, 3wt.% CMC (sodium hydroxymethylcellulose), 0.3wt.% emulsified silicone oil and the balance physiological saline solvent.

[0053] Comparative Example 1

[0054] The Doppler Fluid Model 769DF blood-simulating fluid produced by CIRS Company was used as a comparative example.

[0055] Test Example 1

[0056] The blood-simulating fluid of embodiment 3 is configured as follows: Figure 1 When it is poured outward as shown, it can be observed that the falling fluid is a whole, unseparated liquid column, which means that the fluid has a certain viscosity and is deformable, which are the characteristics of a non-Newtonian fluid.

[0057] Take the syringe and draw the blood-like fluid in the measuring cup. Figure 2 As shown, it can be observed that: when the syringe tip leaves the measuring cup, the fluid in the measuring cup can still be sucked into the syringe, producing a siphon effect, which is a characteristic of non-Newtonian fluid. At the same time, it can also be observed that the diameter of the fluid outside the tip is larger than the diameter of the tip, which is also a characteristic of non-Newtonian fluid.

[0058] Test Example 2

[0059] The imaging of the blood-like fluid in Example 3 under the action of color Doppler ultrasound is as follows: Figure 3 shown.

[0060] Test Example 3

[0061] Figure 4 The acoustic attenuation coefficient curves of the simulated blood fluid of Example 3 and real blood are compared. Figure 4 It can be seen that the acoustic attenuation coefficient of the blood-simulating fluid of the present invention is very close to that of real blood.

[0062] Test Example 4

[0063] The acoustic properties of Examples 1 to 5 and Comparative Example 1 are shown in Table 1.

[0064] Table 1: Acoustic characteristic parameters of blood-simulated fluid

[0065]

[0066] The standard refers to the requirements of IEC61685:2001BMF SPECIFICATION for the characteristics of blood-like fluids

[0067] It can be seen from Table 1 that the blood-simulating fluids of Examples 1 to 5 of the present invention can simulate human blood more realistically than Comparative Example 1.

[0068] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A blood-mimicking fluid, characterized in that: The following components are included in mass percentage: 20wt.% to 40wt.% of polyvinyl pyrrolidone and the balance is solvent.

2. The blood-mimicking fluid according to claim 1, characterized in that: The average molecular weight of the polyvinyl pyrrolidone is 8,000 to 1,300,000.

3. The blood-mimicking fluid according to claim 1, characterized in that: The polyvinyl pyrrolidone includes one or more of PVP K15, PVP K17, PVP K25, PVP K30, PVP K60 and PVP K90.

4. The blood-simulating fluid according to claim 1, characterized in that: The solvent includes one or more of water, glucose and physiological saline.

5. The blood-mimicking fluid according to any one of claims 1 to 4, characterized in that: It also includes 3 wt.% to 5 wt.% of a thickener.

6. The blood-simulating fluid according to claim 5, characterized in that: The thickener includes one or more of sodium hydroxymethyl cellulose, hydroxyethyl methacrylate, and sodium alginate.

7. The blood-simulating fluid according to claim 5, characterized in that: It also includes 0.1 wt.% to 0.5 wt.% of a defoaming agent.

8. The blood-simulating fluid according to claim 7, characterized in that: The defoaming agent includes one or more of emulsified silicone oil and dimethylsiloxane polymer.

9. The blood-simulating fluid according to claim 1, characterized in that: The viscosity of the blood-simulating fluid is 3 cP to 5 cP; The sound velocity of the blood-simulating fluid is 1570m / s to 1600m / s; The acoustic attenuation of the blood-simulating fluid is less than 0.1 dB / cm / MHz; The density of the blood-simulating fluid is 1 g / cc to 1.1 g / cc.

10. A method for preparing a blood-mimicking fluid as claimed in any one of claims 1 to 9, characterized in that: The process includes: Polyvinyl pyrrolidone is dissolved in a solvent to obtain the blood-mimicking fluid.

Images